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{
"id": 8,
"issues": [
{
"id": 26,
"title": "Vol8 Issue3",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2025",
"slug": "2025-vol8-issue3",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": []
},
{
"id": 25,
"title": "Vol8 Issue2",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": true,
"special_issue_deadline": null,
"volume": "2025",
"slug": "2025-vol8-issue2",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 334,
"slug": "178-1736575845-effect-of-fungal-microbiota-on-rankl-and-sclerostin-in-patients-with-crohns-disease",
"type": "original_article",
"manuscript_id": "178-1736575845",
"published": "2025-04-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/56/178-1736575845.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 316-327",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Al-Rubaye AH, Alabassi HM, et al. Effect of fungal microbiota on RANKL and sclerostin in patients with Crohn's disease. J Adv Biotechnol Exp Ther. 2025; 8(2): 316-327.",
"title": "Effect of fungal microbiota on RANKL and sclerostin in patients with Crohn's disease",
"abstract": "<p>The etiology of Crohn's disease (CD) is still unknown. However, many factors, including a dysregulated immune system, altered microbiota, inheritance, and environmental factors, have been implicated. This work was conducted to estimate the effect of fungal microbiota on two bone mineral density markers, RANKL and sclerostin, in addition to the correlation between these markers and vitamin B12, D3, and zinc in CD patients, along with their potential effect on fungal microbiota and vice versa. Peripheral blood and carry-Blair Stool samples were collected from 88 participants (60 newly diagnosed with CD without treatment and 28 healthy controls) to detect serum levels of RANKL and sclerostin, and culture media were used to grow, isolate, and identify fungi attendant to CD and its effect on RANKL and sclerostin levels. Sociodemographic data (vitamin B12, D3, and zinc levels) were collected from patients' medical records. The results showed significant differences in RANKL and sclerostin levels in various types of fungal microbiota in CD patients along with a significant increase in RANKL and sclerostin levels in these patients. Moreover, RANKL levels were negatively significantly correlated with Zinc, while sclerostin levels correlated negatively with vit D3. The findings of this study suggest that fungal microbiota may play a role in the inflammatory process and interactions with bone density by affecting levels of RANKL and sclerostin, vitamin D3, and zinc, suggesting that the use of the fungal microbiota in the monitoring and treatment of CD patients.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.26",
"views": 145,
"downloads": 27,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/43/19/178-1736575845-Figure1.jpg",
"caption": "Figure 1. A) Serum levels of RANKL. B) Serum levels of sclerostin. Mean ± standard error (SE). ** High significant increase in RANKL levels in CD patients compared to control at probability level p≤0.001. *A slightly significant increase in patients with CD compared to controls at probability at probability (P≤0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/43/19/178-1736575845-Figure2.jpg",
"caption": "Figure 2. A) Serum levels of RANKL based on blood groups. B) Serum levels of sclerostin based on blood groups. Data are presented as Mean ± standard error (SE). No significant difference between the mean rank of the RANKL based on blood groups. No significant difference between the mean rank of sclerostin, based on blood groups as demonstrated.",
"featured": false
}
],
"authors": [
{
"id": 1682,
"affiliation": [
{
"affiliation": "Department of Biology, College of Education for Pure Sciences/ Ibn Al-Haitham, University of Baghdad, Iraq"
}
],
"first_name": "Rana Hanan",
"family_name": "Al-Rubaye",
"email": "rana.h.k@ihcoedu.uobaghdad.edu.iq",
"author_order": 1,
"ORCID": "https://orcid.org/0009-0002-2822-712X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Rana Hanan Al-Rubaye, Department of Biology, College of Education for Pure Sciences/ Ibn Al-Haitham, University of Baghdad, Iraq. Email: rana.h.k@ihcoedu.uobaghdad.edu.iq",
"article": 334
},
{
"id": 1683,
"affiliation": [
{
"affiliation": "Department of Biology, College of Education for Pure Sciences/ Ibn Al-Haitham, University of Baghdad, Iraq"
}
],
"first_name": "Hazima Mossa",
"family_name": "Alabassi",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-9218-8762",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 334
},
{
"id": 1684,
"affiliation": [
{
"affiliation": "Department of Analytic Pathology, College of Applied Science, University of Fallujah, Al-Fallujah, Iraq"
}
],
"first_name": "Anwar Khalil",
"family_name": "Ismael",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0001-9031-9856",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 334
},
{
"id": 1685,
"affiliation": [
{
"affiliation": "Cancer Research Department Iraqi center for cancer and Genetic Research, Mustansiryah University, Baghdad, Iraq"
}
],
"first_name": "Alaa Saad",
"family_name": "Hasan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 334
}
]
},
{
"id": 333,
"slug": "178-1740468828-intermittent-androgen-therapy-in-prostate-cancer-reveals-the-pro-apoptotic-roles-of-androgenandrogen-receptor-an-overview",
"type": "review_article",
"manuscript_id": "178-1740468828",
"published": "2025-04-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/04/178-1740468828.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 301-315",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Altuwaijri S, Albarrak SM, et al. Intermittent androgen therapy in prostate cancer reveals the pro-apoptotic roles of androgen/androgen receptor: an overview. J Adv Biotechnol Exp Ther. 2025; 8(2): 301-315.",
"title": "Intermittent androgen therapy in prostate cancer reveals the pro-apoptotic roles of androgen/androgen receptor: an overview",
"abstract": "<p>For the treatment of advanced prostate cancer, either castration alone or in conjunction with androgen ablation is a crucial therapeutic strategy. Patients initially respond favorably to the treatment but eventually reach a hormone-resistant stage known as an androgen refractory tumor, which has an aggressive propensity to spread. Changes in the control of apoptotic pathways have been linked to this development towards androgen unresponsiveness. Reduction in apoptosis sensitivity or elevation in resistance to it seems to be a significant indicator of oncogenic transformation that cannot be treated. Resuming androgen levels after intermittent androgen therapy has been proposed to change tumor cells' growth behavior and make them more sensitive to pro-apoptotic drugs. This review offers an overview of the current understanding of the therapeutic benefits of androgen/androgen receptor-induced apoptotic induction. It also sheds light on the implications of activating novel apoptotic pathways in prostate cancer cells with regard to resistance targeting.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.25",
"views": 148,
"downloads": 24,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/53/09/178-1740468828-Figure1.jpg",
"caption": "Figure 1. The direct mechanism of A/AR induces apoptosis. BRCA 1 is a Tumor suppressor coregulator in androgen-induced apoptosis. BRCA1 stimulates AR transactivation activity by binding directly with AR through two intact pockets within the BRCA 1. This binding is ligand-dependent. BRCA1 can lower the number of cells and raise the proportion of dead cells. BRCA1 can form an important link between androgens/AR for the activation of AR target genes (such as p21) involved in cell cycle control and cell death. Upregulation of p21 in an A/AR-dependent manner enhances the apoptotic response in prostate cancer cells. Retinoblastoma protein (RB) suppresses tumor growth by regulating cell cycle progression. BRCA1 binds preferentially to the hypophosphorylated form of RB and the growth-suppressive phenotype of BRCA1 depends on the presence of a functional RB protein. The transactivational activity of AR and the intact pocket domains of RB are critical determinants for the synergistic functional interaction between AR and RB to induce apoptosis in prostate cancer. RB can only suppress tumors when it is in its active, hypophosphorylated form. Androgens are known to activate RB by inhibiting its phosphorylation at serine 780 (Ser780) and serine 795 (Ser795).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/53/09/178-1740468828-Figure2.jpg",
"caption": "Figure 2. The indirect mechanism of A/AR induces apoptosis. Androgens increase and enhance steady p27 levels, also called cyclin-dependent kinase inhibitor 1B (Cip/Kip 1), through specific modulation of its degradation by SKP2 (Androgens in an AR-dependent manner downregulate SKP2 levels. Elevation of p27 expression in positive AR prostate cancer cell lines by androgens led to reduced cell proliferation. Androgens, at concentrations that inhibit cell growth, suppress c-myc activity in some AR-dependent cell lines. Androgenic control of c-myc levels can be considered a probable mechanism in p27 up-regulation. Bcl-2–associated X protein (Bax), Bcl-2 to Bax ratio in prostate cancer can be an important factor in determining the sensitivity to Bax-induced cell death, Androgens have been shown to reverse this ratio in favor of Bax by downregulating the levels of Bcl2. Up-regulation and modulation of protein kinase C delta (PKCδ) by androgens in an AR-dependent manner serves as a crucial factor in determining the apoptotic response in prostate cancer. Protein kinase C alpha (PKC-α) can induce apoptosis in LNCaP cells by inactivating the survival factor, Akt through modulation of its phosphorylation. upregulation of PKCα by androgens can lead to reduced Akt activity with progression towards cell death. The inhibition of the Akt/PI3k pathway and modification of caspase 8 activity downstream of the death-inducing signaling complex (DISC) underlie TRAIL-dependent stimulation of apoptosis. Androgen ablation in the presence of a PI3k/Akt inhibitor made them resistant to TRAIL treatment. Sensitivity of the cells to TRAIL-induced apoptosis was completely restored in the presence of androgens, suggesting that androgens potentiate the capacity of TRAIL to induce DISC formation which in turn upregulates the expression level of TRAIL R1 and TRAIL R2 conferring them to susceptible to apoptosis. TNF-α and TRAIL are also known as death receptors. When supplemented with androgens demonstrated sensitivity to TNF-α induced apoptosis. AR in a ligand-dependent manner was shown to decrease this resistance by up-regulating TNF-α expression and suppressing NF-kB activity to reduce cell sensitivity to TNF-α mediated apoptosis. Androgens downregulate NF-kB levels by down-regulating IκB kinase (IKK) expression and reducing the phosphorylation of the IkBα protein. This in turn reduces NF-kB's constitutive activity in prostate cancer and its transfer to the nucleus and an increase in the JNK activity, and JNK activation contributes to an increase in caspases 3 to induce apoptosis and Bcl2 inactivation. Androgenic suppression of Bcl-2 expression could involve E2F, androgens/AR down-regulate E2F1 protein levels and decrease the binding of E2F1 to the promoter site in bcl2. As a result, decreasing bcl2.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/53/09/178-1740468828-Figure3.jpg",
"caption": "Figure 3. Androgen ablation is a valuable treatment option for advanced prostate cancer. Patients initially respond well to treatment but eventually advance to a hormone-resistant stage known as an androgen refractory tumor, which is highly likely to metastasize. Resuming androgen levels after intermittent androgen therapy has been proposed to change tumor cell development behavior and make them more vulnerable to pro-apoptotic drugs.",
"featured": true
}
],
"authors": [
{
"id": 1680,
"affiliation": [
{
"affiliation": "Department of Pathology and Laboratory Diagnosis, College of Veterinary Medicine, Qassim University, Buraydah City, Saudi Arabia"
}
],
"first_name": "Saleh",
"family_name": "Altuwaijri",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 333
},
{
"id": 1681,
"affiliation": [
{
"affiliation": "Department of Pathology and Laboratory Diagnosis, College of Veterinary Medicine, Qassim University, Buraydah City, Saudi Arabia"
}
],
"first_name": "Saleh M.",
"family_name": "Albarrak",
"email": "Salbarrak@qu.edu.sa",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-4965-6142",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Saleh M. Albarrak; Department of Pathology and Laboratory Diagnosis, Collage of Veterinary Medicine, Qassim University, Buraydah City, Saudi Arabia. Email: Salbarrak@qu.edu.sa",
"article": 333
}
]
},
{
"id": 332,
"slug": "178-1737300602-animal-model-based-adjunctive-herbal-therapy-in-autism-spectrum-disorder-therapeutic-advances-and-prospects",
"type": "review_article",
"manuscript_id": "178-1737300602",
"published": "2025-03-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/47/178-1737300602.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 283-300",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Toumi ML,Merzoug S, et al. Animal model-based adjunctive herbal therapy in autism spectrum disorder: Therapeutic advances and prospects. J Adv Biotechnol Exp Ther. 2025; 8(2): 283-300.",
"title": "Animal model-based adjunctive herbal therapy in autism spectrum disorder: Therapeutic advances and prospects",
"abstract": "<p>Autism spectrum disorder is a complex neurodevelopmental condition that poses major challenges to caregivers in contemporary societies. Since there is no established cure for this disorder so far, autistic patients of all ages are currently taken in charge of psychological and educational therapies that address their primary complaints while enhancing their quality of life. With the growing interest in herbal therapy globally, especially for illnesses with well-known underlying mechanisms, there have been clinical attempts to treat autism symptomatology using herbs or natural plant molecules in parallel with traditional follow-up. Nevertheless, basic research has only recently focused on studying the effects of herbal extracts in animal models of autism. This review emphasizes the animal studies that may provide credence to the adjunction of herbal therapy to conventional care strategies. Therefore, the study deduced a timeline chart combining promising herbal extracts such as <em>Camellia sinensis, Bacopa monniera</em>, and Korean red ginseng. This allows clinicians and caregivers to further evaluate the positive outcomes reported in autistic-like rodents. In conclusion, the study suggests employing herbal therapy as a clinical adjunct in the context of phytosupportive care.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.24",
"views": 280,
"downloads": 41,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/53/08/178-1737300602-Figure1.jpg",
"caption": "Figure 1. Overall signs and symptoms found in patients with ASD. This complex disease shares many signs and symptoms characteristic of other neuropsychiatric conditions, thus making both the diagnosis and subsequent treatment challenging for caregivers.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/53/08/178-1737300602-Figure2.jpg",
"caption": "Figure 2. A timeline chart of adjunctive herbal therapies in patients with ASD. The time points indicated for each herb correspond to a certain human age, approximately equivalent to the animal's developmental stage. Herbal therapies are independent of each other, and their overlap in time does not signify herbal mixtures. * The dashed line indicates that the treatment endpoint was not specified in the corresponding animal study. The treatment with Clitoria ternatea L. begins at 4 to 8 weeks of age in animals, meaning that the minimum human age for administering this herb is approximately 6.8 years.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/53/08/178-1737300602-Figure3.jpg",
"caption": "Figure 3. Current research relies on animal models that describe the behavioral, histopathological, and molecular aspects of ASD. In autistic-like rodents, herbal treatments have led to symptom improvement, thus opening the way for analytical studies that yield predictive models in males and females, accounting for a sufficiently large sample size. The established models would underlie the implementation of cohort studies and help ultimately approve herbal treatments for clinical use.",
"featured": false
}
],
"authors": [
{
"id": 1677,
"affiliation": [
{
"affiliation": "Laboratory of Functional and Evolutionary Ecology, Department of Biology, Faculty of Natural and Life Sciences, Chadli Bendjedid University, El-Tarf, Algeria"
}
],
"first_name": "Mohamed Lamine",
"family_name": "Toumi",
"email": "toumi-mhd-lamine@univ-eltarf.dz",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-0103-8524",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohamed Lamine Toumi, Laboratory of Functional and Evolutionary Ecology, Department of Biology, Faculty of Natural and Life Sciences, Chadli Bendjedid University, El-Tarf, Algeria.\r\nEmail: toumi-mhd-lamine@univ-eltarf.dz",
"article": 332
},
{
"id": 1678,
"affiliation": [
{
"affiliation": "Laboratory of Functional and Evolutionary Ecology, Department of Biology, Faculty of Natural and Life Sciences, Chadli Bendjedid University, El-Tarf, Algeria"
}
],
"first_name": "Sameha",
"family_name": "Merzoug",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-4940-938X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 332
},
{
"id": 1679,
"affiliation": [
{
"affiliation": "Laboratory of Functional and Evolutionary Ecology, Department of Biology, Faculty of Natural and Life Sciences, Chadli Bendjedid University, El-Tarf, Algeria"
}
],
"first_name": "Ghada",
"family_name": "Senane",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 332
}
]
},
{
"id": 331,
"slug": "178-1731771562-thymoquinone-mitigates-phthalate-induced-reproductive-toxicities-in-male-swiss-albino-mice",
"type": "original_article",
"manuscript_id": "178-1731771562",
"published": "2025-03-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/31/178-1731771562.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 272-282",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Anjir M, Miah MA, et al. Thymoquinone mitigates phthalate-induced reproductive toxicities in male Swiss albino mice. J Adv Biotechnol Exp Ther. 2025; 8(2): 272-282.",
"title": "Thymoquinone mitigates phthalate-induced reproductive toxicities in male Swiss albino mice",
"abstract": "<p>Phthalates (PHA) are common environmental pollutants used extensively in the plastics sector. Exposure to PHA negatively impacts both human and animal health. Thymoquinone (TQ), an active ingredient of black cumin seed, exhibits potential pharmacological effects against many illnesses. The study aimed to investigate how TQ affected the reproductive parameters and histo-morphology of the testes in male Swiss albino mice that has been given PHA. Twenty-four male mice, aged 28 to 30 days, were utilized and allocated into three groups, each including eight animals. Group A (the control) received standard mice pellets combined with olive oil; Group B was supplied PHA; and Group C was given both TQ and PHA mixed with mice pellets. All mice were reared at 26–30°C for 60 days. Blood, serum, and organs were obtained and processed using established protocols. PHA induced an elevation in body weight in male mice. The administration of TQ normalized body weight in PHA-treated mice. Administration of PHA to male mice resulted in a significant decrease in blood levels of thyroxine (T4) and testosterone (p <0.01), whereas the administration of TQ led to an increase in these two hormones. PHA resulted in a substantial (p<0.01) decrease in sperm count and motility, accompanied by an increase in abnormalities, whereas TQ mitigated these sperm characteristics. Degenerative and necrotic alterations were observed in the seminiferous tubules of the testis in PHA-treated male mice which was altered by TQ. In conclusion, the integration of TQ may alleviate the adverse effects generated by PHA.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.23",
"views": 505,
"downloads": 36,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/25/08/178-1731771562-Figure1.jpg",
"caption": "Figure 1. Effect of TQ on body weights (grams) (Mean± SEM) in PHA-treated male mice in a time-dependent manner. Swiss albino mice were treated with TQ and PHA daily for 60 days (details in materials and methods). Body weights were measured at every 15-day interval. * p<0.05 (control versus PHA / PHA+TQ at day 45) ** P<0.01 (control versus PHA/PHA+TQ at day 60 of age).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/25/08/178-1731771562-Figure2.jpg",
"caption": "Figure 2. Effects of TQ on (A) sperm count, (B) sperm motility, and (C) sperm abnormalities in PHA-treated male mice. Swiss albino male mice were treated with TQ and PHA daily for 60 days (details in materials and methods). Testes were isolated at day 60 and analyzed for sperm parameters *p<0.05 PHA+TQ versus PHA); #p<0.01 (control versus PHA); ns = not significant (control versus PHA+TQ).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/25/08/178-1731771562-Figure3.jpg",
"caption": "Figure 3. Representative images of normal and abnormal sperm in male albino mice treated with PHA and TQ. Swiss albino male mice were treated with TQ and PHA daily for 60 days (details in materials and methods). Testes were isolated at day 60; sperms were separated and stained with William staining. A, B, and H represent normal sperms, and D-F, I, and J represent abnormal sperms. C, sperm without a hook and a folded or coiled tail; D, small or immature sperm; E, folded sperm; F, sperm without a head; G, decapacitated sperm; I, two heads with a single tail; and J, an abnormal head with a coiled tail.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/25/08/178-1731771562-Figure4.jpg",
"caption": "Figure 4. Effects of TQ on (A)Testosterone and (B) T4 level in PHA-treated male mice. Swiss albino mice were treated with TQ and PHA daily for 60 days (details in materials and methods). Blood samples were collected on day 60; sera were separated and analyzed for testosterone and T4 by radioimmunoassay. *p<0.05 (control versus PHA+TQ for both and PHA versus PHA+TQ only for T4); ** p<0.01 (control versus PHA for both and PHA versus PHA+TQ only for testosterone).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/25/08/178-1731771562-Figure5.jpg",
"caption": "Figure 5. Effect of TQ on testis in PHA-treated male albino mice. Photomicrograph of histopathological sections of testicular tissues of different groups of mice after 60 days of study. Swiss albino male mice were treated with TQ and PHA daily for 60 days (details in materials and methods). Testis was isolated at day 60; formalin-fixed, sectioned, and stained with Hematoxylin and Eosin staining non-treated control (A1, A2); PHA (B1, B2); PHA+TQ (C1, C2) at 100X and 400X magnification. S, spermatozoa; SG, spermatogonium.",
"featured": true
}
],
"authors": [
{
"id": 1671,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
},
{
"affiliation": "Department of Physiology, Pharmacology & Toxicology, Habiganj Agricultural University, Habiganj-3300, Bangladesh"
}
],
"first_name": "Musfika",
"family_name": "Anjir",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 331
},
{
"id": 1672,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mohammad Alam",
"family_name": "Miah",
"email": "mam74@bau.edu.bd",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-2778-9614",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Alam Miah, Department of Physiology Bangladesh Agricultural University Mymensingh-2202, Bangladesh. Email: mam74@bau.edu.bd",
"article": 331
},
{
"id": 1673,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sajibul",
"family_name": "Hasan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 331
},
{
"id": 1674,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Afrina",
"family_name": "Mustari",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 331
},
{
"id": 1675,
"affiliation": [
{
"affiliation": "Department of Pathology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md. Abu Hadi Noor Ali",
"family_name": "Khan",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 331
},
{
"id": 1676,
"affiliation": [
{
"affiliation": "Department of Surgery & Obstetrics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Farida Yeasmin",
"family_name": "Bari",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 331
}
]
},
{
"id": 330,
"slug": "178-1733198863-development-of-gelatin-particles-encapsulating-pdgf-bb-aptamer-for-the-efficient-drug-delivery-system",
"type": "original_article",
"manuscript_id": "178-1733198863",
"published": "2025-03-17",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/11/178-1733198863.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 259-271",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah,\r\nUSA",
"cite_info": "Pikulthong W, Aunkitkancharoen P, et al. Development of gelatin particles encapsulating PDGF-BB aptamer for the efficient drug delivery system. J Adv Biotechnol Exp Ther. 2025; 8(2): 259-271.",
"title": "Development of gelatin particles encapsulating PDGF-BB aptamer for the efficient drug delivery system",
"abstract": "<p>Drug delivery systems (DDSs) improve therapy through controlled release and targeted delivery, utilizing recognition elements, therapeutic agents, and carriers. Among various recognition elements, aptamers have gained attention for their high affinity and specificity. Gelatin, a biocompatible and biodegradable material, shows potential for DDSs due to its favorable properties. This study investigated gelatin-based particles for aptamer delivery, focusing on their preparation, characterization, and evaluation. Gelatin particles loaded with platelet-derived growth factor-BB (PDGF-BB) aptamer were prepared using glutaraldehyde as a crosslinker, with sodium dodecyl sulfonate (SDS) and Tween 20 as stabilizers. The particles were characterized through spectroscopy techniques, dynamic light scattering (DLS), and scanning electron microscopy (SEM). The reaction between aldehyde and amine groups was confirmed, and the effect of surfactants on particle formation was examined. Aptamer release and stability were also studied. The ninhydrin assay and Fourier-transform infrared (FTIR) analysis confirmed successful crosslinking between gelatin and glutaraldehyde, forming stable particles. Zeta potential measurements showed that both SGP and TGP maintained stability within the range of -30 to -60 mV. Particle size analysis via DLS and SEM revealed that increasing surfactant concentration led to larger particles, with sizes ranging from 95 to 120 nm. Weight loss studies demonstrated that SGP followed zero-order kinetics while TGP exhibited first-order degradation, with both retaining over 90% of their weight within 72 hours. Aptamer release studies showed that SGP had a higher release rate than TGP, correlating with their degradation profiles. Finally, electrophoresis confirmed the integrity of released aptamers, emphasizing the protective role of gelatin particles. Overall, gelatin-based particles show promise for aptamer delivery, offering stability and controlled release. These findings contribute to the advancement of effective DDSs for targeted therapeutic delivery.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.22",
"views": 199,
"downloads": 59,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/57/07/178-1733198863-Figure1.jpg",
"caption": "Figure 1. Quantification of amine groups on particles using ninhydrin assay: UG denotes unmodified gelatin, while SGP and TGP represent particles synthesized in the presence of SDS and Tween 20, respectively. The error bars indicate one standard deviation (n=3). *P < 0.05 indicates a significant difference.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/57/07/178-1733198863-Figure2.jpg",
"caption": "Figure 2. FTIR spectra of UG, SGP, and TGP indicate functional groups available on the gelatin particles. UG denotes unmodified gelatin, while SGP and TGP represent particles synthesized in the presence of SDS and Tween 20, respectively. %T refers to percent transmittance.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/57/07/178-1733198863-Figure3.jpg",
"caption": "Figure 3. Effect of surfactant concentration to CMC ratio on particle zeta potential. The gelatin particles were suspended in DI water. CMC denotes critical micelle concentration. The error bars indicate one standard deviation (n=3). *P < 0.05 indicates a significant difference.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/57/07/178-1733198863-Figure4.jpg",
"caption": "Figure 4. Effect of surfactant concentration to CMC ratio on particle diameter. The gelatin particles were suspended in DI water. CMC denotes critical micelle concentration. The error bars indicate one standard deviation (n=3). *P < 0.05 indicates a significant difference.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/57/07/178-1733198863-Figure5.jpg",
"caption": "Figure 5. SEM images of gelatin particles prepared at varying surfactant concentration to CMC ratios. The particles were coated with gold and observed at 40,000x magnification. The upper row depicts SGP at (A) 10, (B) 2, and (C) 0.4 ratios. The lower row illustrates TGP at (D) 10, (E) 2, and (F) 0.4 ratios. The scale bar indicates 100 nm in length.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/57/07/178-1733198863-Figure6.jpg",
"caption": "Figure 6. Weight loss of SGP and TGP. The particles were incubated in PBS at 25°C. At set intervals, suspensions were centrifuged, and the particles were removed, vacuum-dried overnight, and weighed to assess weight loss. The error bars indicate one standard deviation (n=3).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/57/07/178-1733198863-Figure7.jpg",
"caption": "Figure 7. Cumulative release of PDGF-BB aptamer from SGP and TGP. The gelatin particles were incubated in PBS at 25°C. At set intervals, 100 µL of medium was sampled and replaced with fresh PBS. Aptamer concentration was measured via spectrophotometry. The error bars indicate one standard deviation (n=3).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/57/07/178-1733198863-Figure8.jpg",
"caption": "Figure 8. Gel electrophoresis of PDGF-BB aptamer release from (A) SGP and (B) TGP at specified time points. The aptamer solution was collected at each specified time during the release study.",
"featured": true
}
],
"authors": [
{
"id": 1663,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand"
}
],
"first_name": "Wannapha",
"family_name": "Pikulthong",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 330
},
{
"id": 1664,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand"
}
],
"first_name": "Phattharawadee",
"family_name": "Aunkitkancharoen",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 330
},
{
"id": 1665,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand"
}
],
"first_name": "Wilawan",
"family_name": "Chaothum",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 330
},
{
"id": 1666,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand"
}
],
"first_name": "Napassawan",
"family_name": "Jorntrakan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 330
},
{
"id": 1667,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand"
},
{
"affiliation": "National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathumthani, Thailand"
}
],
"first_name": "Kanpitcha",
"family_name": "Jiramitmongkon",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 330
},
{
"id": 1668,
"affiliation": [
{
"affiliation": "Division of Biochemistry, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani, Thailand"
},
{
"affiliation": "Thammasat University Research Unit in Innovation of Molecular Hybrid for Biomedical Application, Pathumthani, Thailand"
}
],
"first_name": "Pichayanoot",
"family_name": "Rotkrua",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0003-2672-5782",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 330
},
{
"id": 1669,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand"
},
{
"affiliation": "Thammasat University Research Unit in Innovation of Molecular Hybrid for Biomedical Application, Pathumthani, Thailand"
}
],
"first_name": "Jiraporn",
"family_name": "Arunpanichlert",
"email": null,
"author_order": 7,
"ORCID": "https://orcid.org/0000-0003-3914-213X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 330
},
{
"id": 1670,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand"
},
{
"affiliation": "Thammasat University Research Unit in Innovation of Molecular Hybrid for Biomedical Application, Pathumthani, Thailand"
}
],
"first_name": "Boonchoy",
"family_name": "Soontornworajit",
"email": "sbooncho@tu.ac.th",
"author_order": 8,
"ORCID": "https://orcid.org/0000-0002-2680-3456",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Boonchoy Soontornworajit Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani ,Thailand. \r\nEmail: sbooncho@tu.ac.th",
"article": 330
}
]
},
{
"id": 329,
"slug": "178-1733537671-bovine-viral-diarrhea-virus-antigen-status-in-milk-and-blood-serum-implications-for-effective-screening-and-risk-factors-analysis-in-dairy-cows",
"type": "short_communication",
"manuscript_id": "178-1733537671",
"published": "2025-03-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/13/178-1733537671.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 251-258",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Ullah MA, Hasan SMN, et al. Bovine viral diarrhea virus antigen status in milk and blood serum: Implications for effective screening and risk factors analysis in dairy cows. J Adv Biotechnol Exp Ther. 2025; 8(2): 251-258.",
"title": "Bovine viral diarrhea virus antigen status in milk and blood serum: Implications for effective screening and risk factors analysis in dairy cows",
"abstract": "<p>Bovine viral diarrhea virus (BVDV) is a significant viral pathogen affecting cow populations worldwide. This study was conducted in the Bagha Bari area of Sirajganj District, Bangladesh, and investigated the prevalence of BVDV antigen in 54 dairy cattle exhibiting reproductive, milk production, and congenital abnormalities. Milk and blood samples were collected from dairy cows without a history of BVDV vaccination. The research work employs indirect enzyme-linked immunosorbent assay (ELISA) to detect viral antigen, and the results reveal a significant discrepancy in BVDV antigen positivity between milk (55.56%) and serum (92.60%) samples from the same animals. Interestingly, animals negative in one sample type tested positive in the other, indicating potential localized viral presence or shedding variations. ELISA titers differed between serum and milk samples, highlighting variations in viral dynamics within bodily fluids. Risk factor analysis shows an association between BVDV positivity and pregnancy. In conclusion, this study advocates a comprehensive testing approach involving both serum and milk samples for effective BVDV screening. Future studies should involve larger, geographically varied populations and incorporate molecular diagnostic tools, such as PCR, to enhance accuracy.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.21",
"views": 203,
"downloads": 39,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/06/22/178-1733537671-Figure1.jpg",
"caption": "Figure 1. The study area map was created by ArcGIS Pro (ESRI, USA) based on the Geographical Information System (GIS).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/06/22/178-1733537671-Figure2.jpg",
"caption": "Figure 2. BVDV antigen status in milk and serum samples of the BVDV suspected cows and their prevalence. A) BVDV Ag ELISA was conducted according to the manufacturer's guidelines, and OD values were recorded. B) The percentages of positive and negative cows based on milk and serum samples.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/13/22/178-1733537671-Figure3.jpg",
"caption": "Figure 3. Risk factors and clinical signs associated with BVDV infected cows include A) age, B) Pregnancy, C) milk production, and D) Congenital effects. Data were collected as mentioned in the questionnaire, and ELISA was conducted according to manufacturer’s guidelines, and samples exhibiting S-N values of 0.277 or lower were categorized as negative for BVDV antigen, while samples with S-N values >0.277 were classified as positive. Data were prepared in MS Office Excel, and a Chi-square test was performed for statistical significance analysis.",
"featured": false
}
],
"authors": [
{
"id": 1656,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md Ashek",
"family_name": "Ullah",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 329
},
{
"id": 1657,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "SM Nazmul",
"family_name": "Hasan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 329
},
{
"id": 1658,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md. Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 329
},
{
"id": 1659,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Nurejunnati",
"family_name": "Jeba",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 329
},
{
"id": 1660,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Sharmin",
"family_name": "Akter",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 329
},
{
"id": 1661,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Sukumar",
"family_name": "Saha",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 329
},
{
"id": 1662,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md. Golzar",
"family_name": "Hossain",
"email": "mghossain@bau.edu.bd",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-1487-5444",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Golzar Hossain, PhD; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.\r\nEmail: mghossain@bau.edu.bd",
"article": 329
}
]
},
{
"id": 328,
"slug": "178-1734789996-prevalence-of-eating-disorders-and-their-association-with-depression-anxiety-and-stress-among-high-school-students-in-suburban-areas-in-vietnam",
"type": "original_article",
"manuscript_id": "178-1734789996",
"published": "2025-03-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/01/178-1734789996.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 242-250",
"academic_editor": "Md. Mahbubur Rahman, PhD; Gachon University Cancer and Diabetes Institute, South Korea",
"cite_info": "Anh TH, Truc LTT, et al. Prevalence of eating disorders and their association with depression, anxiety, and stress among high school students in suburban areas in Vietnam. J Adv Biotechnol Exp Ther. 2025; 8(2): 242-250.",
"title": "Prevalence of eating disorders and their association with depression, anxiety, and stress among high school students in suburban areas in Vietnam",
"abstract": "<p>Eating disorders are a growing concern among adolescents, often associated with mental health issues like depression, anxiety, and stress. Limited evidence exists on their prevalence and contributing factors in suburban areas in Vietnam. This study aimed to assess the prevalence of eating disorders and their relationship with mental health factors among high school students in these areas. A descriptive cross-sectional survey was conducted with 426 high school students from suburban areas of major Vietnamese cities. The EAT-26 (eating attitudes test-26) and BITE (bulimic investigatory test Edinburgh) questionnaires were used to evaluate eating disorders, while the DASS-21 scale measured levels of depression, anxiety, and stress. The findings showed that 57.7% of participants exhibited signs of eating disorders. Logistic regression analysis identified significant associations between eating disorders and gender, eating habits, body image concerns, and mental health indicators (p-value < 0.05). In conclusion, this study highlights the high prevalence of eating disorders among suburban high school students in Vietnam. The strong associations with gender, eating habits, body image, and mental health factors emphasize the urgent need for targeted interventions and mental health support tailored to this vulnerable population.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.20",
"views": 260,
"downloads": 60,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/05/20/178-1734789996-Figure1.jpg",
"caption": "Figure 1. Eating disorder characteristics of participants. n = 426.",
"featured": false
}
],
"authors": [
{
"id": 1649,
"affiliation": [
{
"affiliation": "Pre-Medical Post-Baccalaureate Students, Bryn Mawr College, USA"
}
],
"first_name": "Ton Hien",
"family_name": "Anh",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 328
},
{
"id": 1650,
"affiliation": [
{
"affiliation": "Nutritional Science student, Institute of Preventive Medicine and Public Health, Hanoi Medical University, Vietnam"
}
],
"first_name": "Le Thi Thanh",
"family_name": "Truc",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 328
},
{
"id": 1651,
"affiliation": [
{
"affiliation": "Nutritional Science student, Institute of Preventive Medicine and Public Health, Hanoi Medical University, Vietnam"
}
],
"first_name": "Nguyen Dieu",
"family_name": "Linh",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 328
},
{
"id": 1652,
"affiliation": [
{
"affiliation": "Nutritional Science student, Institute of Preventive Medicine and Public Health, Hanoi Medical University, Vietnam"
}
],
"first_name": "Nguyen Thu",
"family_name": "Phuong",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 328
},
{
"id": 1653,
"affiliation": [
{
"affiliation": "Nutritional Science student, Institute of Preventive Medicine and Public Health, Hanoi Medical University, Vietnam"
}
],
"first_name": "Nguyen My",
"family_name": "Le",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 328
},
{
"id": 1654,
"affiliation": [
{
"affiliation": "Nutritional Science student, Institute of Preventive Medicine and Public Health, Hanoi Medical University, Vietnam"
}
],
"first_name": "Nguyen Mai",
"family_name": "Phuong",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 328
},
{
"id": 1655,
"affiliation": [
{
"affiliation": "Department of Research Methodology and Biostatistics, Institute of Preventive Medicine and Public Health, Hanoi Medical University, Vietnam"
}
],
"first_name": "Le Xuan",
"family_name": "Hung",
"email": "lexuanhung@hmu.edu.vn",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-1957-8529",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Le Xuan Hung, Department of Research Methodology and Biostatistics, Institute of Preventive Medicine and Public Health, Hanoi Medical University, Vietnam.\r\nE‑mail: lexuanhung@hmu.edu.vn",
"article": 328
}
]
},
{
"id": 327,
"slug": "178-1728471196-diversity-and-resistance-profile-of-bacteria-associated-with-washroom-surfaces-in-bangladesh-agricultural-university-residence-halls",
"type": "original_article",
"manuscript_id": "178-1728471196",
"published": "2025-03-04",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/50/178-1728471196.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 232-241",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Islam MS, Rimi SS, et al. Diversity and resistance profile of bacteria associated with washroom surfaces in Bangladesh Agricultural University residence halls. J Adv Biotechnol Exp Ther. 2025; 8(2): 232-241.",
"title": "Diversity and resistance profile of bacteria associated with washroom surfaces in Bangladesh Agricultural University residence halls",
"abstract": "<p>Public washrooms in shared spaces, such as university residence halls, serve as potential reservoirs for pathogenic and multidrug-resistant bacteria, posing significant public health risks. This study aimed to assess bacterial diversity and evaluate antibiotic resistance profiles on commonly touched surfaces in washrooms and toilets of BAU residence halls. In total, 80 swab samples were obtained from the toilet and bathroom surfaces. Bacterial load was determined from each sample by total viable count (TVC), total coliform count (TCC), and total staphylococcal count (TSC). The bacterial isolates were identified using staining, biochemical testing, and subsequent molecular identification. Afterward, thirteen commonly available antibiotics were used to investigate the antibiotic sensitivity of the isolated organisms by disk diffusion methods. In the washroom samples, the greatest mean values were for TVC (log 5.91), TCC (log 5.75), and TSC (log 5.96). On the other hand, the toilet samples had the lowest mean values for TVC (log 5.39), TCC (log 5.13), and TSC (log 5.47). Notably, the floor surface samples had the highest levels of TVC, TCC, and TSC. The overall prevalence of <em>E. coli</em>, <em>Staphylococcus aureus</em>, and <em>Klebsiella</em> spp. was found to be 71%, 93.75%, and 87.5%. All isolated bacteria were found to be sensitive to chloramphenicol and gentamycin and resistant to ampicillin and amoxicillin. The study also found Methicillin-Resistant <em>Staphylococcus aureus</em> (MRSA), which poses a risk to public health. Therefore, the findings of this study can guide better hygiene practices, antibiotic usage, infrastructural improvements, and MRSA control in shared restrooms.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.19",
"views": 1048,
"downloads": 49,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/07/04/178-1728471196-Figure1.jpg",
"caption": "Figure 1. Agarose gel electrophoresis results of PCR amplification targeting the (A) malB [585 bp] and (B) stx-1 [606 bp] gene of E. coli isolates, (C) Kleb_gyrA [441 bp] gene of Klebsiella spp. isolates, (D) nuc [279 bp] gene of Staphylococcus aureus isolates. In all cases, Lane 1: 100 bp DNA ladder; blank lane represents negative control; and lanes with specific band represent positive isolates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/07/04/178-1728471196-Figure2.jpg",
"caption": "Figure 2. Sensitivity pattern of selected (A) E. coli, (B) Klebsiella spp., and (C) Staphylococcus aureus isolates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/07/04/178-1728471196-Figure3.jpg",
"caption": "Figure 3. Agarose gel electrophoresis results of PCR amplification targeting the (A) tetA [831 bp] gene of E. coli isolates and (B) mecA gene of Staphylococcus aureus isolates. In all cases, Lane 1: 100 bp DNA ladder; blank lane represents negative control; and lanes with specific band represent positive isolates.",
"featured": false
}
],
"authors": [
{
"id": 1642,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Shafiqul",
"family_name": "Islam",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-2390-737X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 327
},
{
"id": 1643,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sabrina Sultana",
"family_name": "Rimi",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0009-0003-4388-0212",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 327
},
{
"id": 1644,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Nahid",
"family_name": "Ashraf",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0009-0003-3243-6191",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 327
},
{
"id": 1645,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sanzila Hossain",
"family_name": "Sigma",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0009-0008-4607-5610",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 327
},
{
"id": 1646,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sharika",
"family_name": "Jahan",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0009-0002-3116-8917",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 327
},
{
"id": 1647,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mahbubul Pratik",
"family_name": "Siddique",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0001-7159-0747",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 327
},
{
"id": 1648,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Tanvir",
"family_name": "Rahman",
"email": "tanvirahman@bau.edu.bd",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0001-5432-480X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Tanvir Rahman, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.\r\nEmail: tanvirahman@bau.edu.bd",
"article": 327
}
]
},
{
"id": 326,
"slug": "178-1730828138-selection-of-efficient-broiler-strain-for-productive-performances-and-immunity-under-local-farming-system-in-bangladesh",
"type": "original_article",
"manuscript_id": "178-1730828138",
"published": "2025-03-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/24/178-1730828138.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 218-231",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Rahman M, Hossain H, et al. Selection of efficient broiler strain for productive performances and immunity under local farming system in Bangladesh. J Adv Biotechnol Exp Ther. 2025; 8(2): 218-231.",
"title": "Selection of efficient broiler strain for productive performances and immunity under local farming system in Bangladesh",
"abstract": "<p>The poultry sector has emerged as an important contributor to the national economy of Bangladesh, meeting essential protein needs with quality products and progressing steadily toward a poultry-sufficient nation. This study investigated the performance of various broiler strains Cobb 500, Indian River (IR), Ross 308, and Efficiency Plus (EP) under the local farming system in Bangladesh, with a focus on growth, carcass characteristics, and immune response. A randomized controlled trial (RCT) was performed using 240 chicks for 42-day experimental trials. Data were collected weekly on body weight, feed intake, carcass yield, and humoral immune responses. These immune responses were evaluated using the hemagglutination inhibition (HI) test and the enzyme-linked immunosorbent assay (ELISA). The key findings indicated that Cobb 500 and Ross 308 exhibited superior growth performance, with Cobb 500 achieving the highest final body weight (2820.67 g) and breast weight (708.00 g). Ross 308 demonstrated a superior feed conversion ratio (FCR) and immune response, exhibiting higher antibody titers against Newcastle Disease and H9N2. Notably, while Ross displayed initially higher antibody levels for Infectious Bursal Disease, Cobb 500 ultimately surpassed it by day 42. Both Cobb 500 and Ross 308 were shown to have strong performance traits, with Cobb 500 leading in body weight and carcass characteristics, while Ross 308 excelled in feed efficiency and immune response. These findings underscored the importance of selecting broiler strains based on comprehensive performance metrics to enhance production efficiency in the poultry sector of Bangladesh. <strong> </strong></p>",
"DOI": "10.5455/jabet.2025.18",
"views": 845,
"downloads": 54,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/13/04/178-1730828138-Figure1.jpg",
"caption": "Figure 1. Comparative analysis of different performance parameters among different broiler strain. Broiler Performance Efficiency Factor (BPEF); Broiler Farm Economy Index (BFEI).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/13/04/178-1730828138-Figure2.jpg",
"caption": "Figure 2. Comparative evaluation of immune (Humoral) response against NDV and avian influenza virus (AIV) among the different strains. A) Evaluation of immune titer against ND vaccine, B) Evaluation of immune titer against H5N1, C) Evaluation of immune titer against H9N2; (ANOVA, *p<0.05, **p<0.01, ***p<0.001; ns: non-significant)",
"featured": false
}
],
"authors": [
{
"id": 1630,
"affiliation": [
{
"affiliation": "Department of Dairy & Poultry Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh"
},
{
"affiliation": "Faculty of Veterinary and Animal Sciences, Gono Bishwabidyalay, Dhaka, Bangladesh"
}
],
"first_name": "Mostafizor",
"family_name": "Rahman",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0007-7815-9010",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1631,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Sylhet Agricultural University, Sylhet, Bangladesh"
}
],
"first_name": "Hemayet",
"family_name": "Hossain",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-9785-2549",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1632,
"affiliation": [
{
"affiliation": "Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh"
}
],
"first_name": "Milon",
"family_name": "Mia",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0009-0007-8113-237X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1633,
"affiliation": [
{
"affiliation": "Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh"
}
],
"first_name": "Md Jamilur",
"family_name": "Rahman",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0009-0001-0870-6919",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1634,
"affiliation": [
{
"affiliation": "Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh"
}
],
"first_name": "Abu Al",
"family_name": "Farabi",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0009-0004-9643-7262",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1635,
"affiliation": [
{
"affiliation": "Department of Microbiology and Public Health, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh"
}
],
"first_name": "Zhokhar",
"family_name": "Dudayev",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-8186-5828",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1636,
"affiliation": [
{
"affiliation": "Department of Zoology (GSSC), University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Khadiza Akter",
"family_name": "Brishty",
"email": null,
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-4594-7703",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1637,
"affiliation": [
{
"affiliation": "Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh"
}
],
"first_name": "Nadia",
"family_name": "Afrin",
"email": null,
"author_order": 8,
"ORCID": "https://orcid.org/0009-0003-4888-6154",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1638,
"affiliation": [
{
"affiliation": "Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh"
}
],
"first_name": "Md. Arif Ishtiaq",
"family_name": "Shovon",
"email": null,
"author_order": 9,
"ORCID": "https://orcid.org/0009-0002-0944-6618",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1639,
"affiliation": [
{
"affiliation": "Department of Medicine, Sylhet Agricultural University, Sylhet, Bangladesh"
}
],
"first_name": "Md. Shahidur Rahman",
"family_name": "Chowdhury",
"email": null,
"author_order": 10,
"ORCID": "https://orcid.org/0000-0002-4594-7703",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1640,
"affiliation": [
{
"affiliation": "Department of Pathology, Sylhet Agricultural University, Sylhet, Bangladesh"
}
],
"first_name": "Md. Masudur",
"family_name": "Rahman",
"email": null,
"author_order": 11,
"ORCID": "https://orcid.org/0000-0001-7095-9303",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 326
},
{
"id": 1641,
"affiliation": [
{
"affiliation": "Department of Medicine, Sylhet Agricultural University, Sylhet, Bangladesh"
}
],
"first_name": "Md. Mahfujur",
"family_name": "Rahman",
"email": "mahfuj.vetmed@sau.ac.bd",
"author_order": 12,
"ORCID": "https://orcid.org/0000-0003-2062-8471",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Mahfujur Rahman, PhD; Department of Medicine, Sylhet Agricultural University, Sylhet, Bangladesh.\r\nEmail: mahfuj.vetmed@sau.ac.bd",
"article": 326
}
]
},
{
"id": 325,
"slug": "178-1730094477-a-comprehensive-study-on-ph-sensitive-nanoparticles-for-the-efficient-delivery-of-drugs",
"type": "review_article",
"manuscript_id": "178-1730094477",
"published": "2025-03-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/24/178-1730094477.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(2): 200-217",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Bachta P, Jakhmola V, et al. A comprehensive study on pH-sensitive nanoparticles for the efficient delivery of drugs. J Adv Biotechnol Exp Ther. 2025; 8(2): 200-217.",
"title": "A comprehensive study on pH-sensitive nanoparticles for the efficient delivery of drugs",
"abstract": "<p>pH-sensitive nanoparticles are smart nanoparticles created to respond to changes in the pH of their surroundings. These nanoparticles contain polymers that undergo structure or chemical change in response to acidic and alkaline conditions. Their ability to alter their characteristics, including size, charge, and solubility, depends upon pH variation. This makes them highly useful in various applications, including targeted drug delivery, disease examination, improving therapeutic efficacy, and reducing systemic toxicity. pH-sensitive nanoparticles can release medication in a specific site with abnormal pH levels, such as a tumor or inflamed area. It also improved oral bioavailability and enhanced the residence time in the gastrointestinal tract, mucoadhesion, permeability of the gut, increased solubility, and faster dissolution of weakly soluble medications. This review article aims to explore the latest advancements in drug development and application of drug delivery systems, focusing on the mechanism of pH-sensitive Nanoparticles and their different types, as well as the challenges, limitations, and prospects in improving their efficacy for targeted and controlled drug release.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.17",
"views": 738,
"downloads": 64,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/41/03/178-1730094477-Figure1.jpg",
"caption": "Figure 1. Response modes of pH-sensitive nanoparticles for targeted drug delivery as previously described [15].",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/41/03/178-1730094477-Figure2.jpg",
"caption": "Figure 2. pH-sensitive nanoparticle for targeted drug release at tumor cells.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/41/03/178-1730094477-Figure3.jpg",
"caption": "Figure 3. The summary demonstrates the role of pH-sensitive nanoparticles in drug delivery. At the top, it outlines the response mechanisms of these nanoparticles, including physical dissociation, nanoparticle swelling, chemical bond cleavage, and the release of therapeutic agents triggered by bond breakdown. On the left, the diagram focuses on the various applications of these nanoparticles, such as tumor targeting, gastroretentive drug delivery, immunotherapy, biosensing, and gene carriers. The right side illustrates the different levels at which pH-sensitive nanoparticles function: at the organ level, transversal region, and at the tissue level, where they specifically accumulate in the acidic tumor microenvironment. Finally, the bottom section depicts how the drug delivery process works, emphasizing the selective release of the drug payload in the tumor site, thereby improving therapeutic outcomes while minimizing side effects to healthy tissues.",
"featured": false
}
],
"authors": [
{
"id": 1621,
"affiliation": [
{
"affiliation": "Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, Dehradun, India"
}
],
"first_name": "Pallavi",
"family_name": "Bachta",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 325
},
{
"id": 1622,
"affiliation": [
{
"affiliation": "Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, Dehradun, India"
}
],
"first_name": "Vikas",
"family_name": "Jakhmola",
"email": "Jakhmola.1979@gmail.com",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Vikas Jakhmola, PhD; Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, Dehradun, India.\r\nEmail: Jakhmola.1979@gmail.com",
"article": 325
},
{
"id": 1623,
"affiliation": [
{
"affiliation": "Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, Dehradun, India"
}
],
"first_name": "Nidhi",
"family_name": "Nainwal",
"email": "nidhi.nainwal87@gmail.com",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-0117-1701",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nidhi Nainwal, Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, Dehradun, India.\r\nEmail: nidhi.nainwal87@gmail.com",
"article": 325
},
{
"id": 1624,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, School of Health Sciences & Technology, University of Petroleum and Energy Studies, Bidholi, Premnagar, Dehradun 248007, Uttarakhand, India"
}
],
"first_name": "Poonam",
"family_name": "Joshi",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 325
},
{
"id": 1625,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, School of Health Sciences & Technology, University of Petroleum and Energy Studies, Bidholi, Premnagar, Dehradun 248007, Uttarakhand, India"
}
],
"first_name": "Richa",
"family_name": "Bahuguna",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0001-8408-9724",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 325
},
{
"id": 1626,
"affiliation": [
{
"affiliation": "School of Pharmaceutical Sciences, Shri Guru Ram Rai University, Dehradun, India"
}
],
"first_name": "Meenu",
"family_name": "Chaudhary",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 325
},
{
"id": 1627,
"affiliation": [
{
"affiliation": "Samrat Prithviraj Chauhan College of Pharmacy, Kashipur, Uttarakhand Technical University, Uttarakhand, India"
}
],
"first_name": "Kapil",
"family_name": "Kumar",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 325
},
{
"id": 1628,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, Amrapali University Haldwani, Uttarakhand, India"
}
],
"first_name": "Arun Kumar",
"family_name": "Singh",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 325
},
{
"id": 1629,
"affiliation": [
{
"affiliation": "Himalayan Institute of Pharmacy and Research, Dehradun, India"
}
],
"first_name": "Praveen",
"family_name": "Kumar",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 325
}
]
}
]
},
{
"id": 24,
"title": "Vol8 Issue1",
"cover": "https://jabet.bsmiab.org/media/issue_cover/Jabet_Coverpage_29atpM6.jpg",
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": true,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2025",
"slug": "2025-vol8-issue1",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 324,
"slug": "178-1724280161-a-combination-of-ursodeoxycholic-acid-and-allium-sativum-prevents-liver-cirrhosis-in-cholestatic-rats",
"type": "original_article",
"manuscript_id": "178-1724280161",
"published": "2025-01-28",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/45/178-1724280161.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 192-199",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Adhuri MN, Susilaningsih N, et al. A combination of ursodeoxycholic acid and Allium sativum prevents liver cirrhosis in cholestatic rats. J Adv Biotechnol Exp Ther. 2025; 8(1): 192-199",
"title": "A combination of ursodeoxycholic acid and <span>Allium sativum</span> prevents liver cirrhosis in cholestatic rats",
"abstract": "<p>Cholestasis causes liver cirrhosis due to chronic total obstruction, in which accumulated bile acids induce liver inflammation and fibrosis. Inflammatory conditions are associated with hypoalbuminemia, and changes in albumin levels indicate the patient's clinical condition. <em>Allium sativum</em> has anti-inflammatory, antioxidant, and antifibrotic effects against various diseases. Further, ursodeoxycholic acid (UDCA) is one of the few available therapies for cirrhosis. Thus, this study aimed to assess the combination of <em>A. sativum</em> and UDCA as standard therapy in preventing liver cirrhosis and cholestasis. The current study was carried out with 35 male Sprague Dawley rats, which were randomly assigned into healthy, negative, and positive control groups, and three treatments of UDCA and graded doses of <em>A. sativum</em> extract. Biliary duct ligation was used for inducing cholestasis, and the treatment was given for 21 days. Liver cirrhosis was assessed based on Laennec grade by histopathological examination. Albumin levels are measured by examining blood serum. The combination of UDCA and <em>A. sativum</em> extract significantly reduced the degree of liver fibrosis and increased albumin levels in mice with cholestasis (p<0.05). There was no difference in the degree of liver fibrosis between the graded doses of <em>A. sativum</em>. Albumin levels were significantly correlated with the degree of liver fibrosis (p<0.05). In conclusion, the data suggest that <em>A. sativum</em> administration can be combined with UDCA to increase the hepatoprotective effect against cholestasis in rats.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.16",
"views": 495,
"downloads": 78,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/35/30/178-1724280161-Figure1.jpg",
"caption": "Figure 1. Microscopic evaluation of liver cirrhosis degree with 40x magnification. A) grade 0 (no fibrosis); B) grade 1 (minimal fibrosis); C) grade 2 (mild fibrosis); D) grade 3 (moderate fibrosis); E) grade 4A (cirrhosis, mild definite or probable); F) 4B (moderate cirrhosis); The appearance of septa (arrowhead).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/35/30/178-1724280161-Figure2.jpg",
"caption": "Figure 2. Effect of UDCA and A. sativum extract on albumin level. Results are expressed as median, interquartile range, maximum, and minimum values. Designations: p < 0.05 vs * BDL; # BDL vs others.",
"featured": false
}
],
"authors": [
{
"id": 1616,
"affiliation": [
{
"affiliation": "Biomedical Science Postgraduate Program, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Pratama Nurmalik",
"family_name": "Adhuri",
"email": "pratama.adhuri@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Pratama Nurmalik Adhuri, Biomedical Science Postgraduate Program, Faculty of Medicine, Diponegoro University, Semarang, Indonesia. Email: pratama.adhuri@gmail.com",
"article": 324
},
{
"id": 1617,
"affiliation": [
{
"affiliation": "Department of Histology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Neni",
"family_name": "Susilaningsih",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 324
},
{
"id": 1618,
"affiliation": [
{
"affiliation": "Department of Digestive Surgery, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Sigit Adi",
"family_name": "Prasetyo",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 324
},
{
"id": 1619,
"affiliation": [
{
"affiliation": "Department of Pharmacology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Nani",
"family_name": "Maharani",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 324
},
{
"id": 1620,
"affiliation": [
{
"affiliation": "Department of Pharmacology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Trilaksana",
"family_name": "Nugroho",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 324
}
]
},
{
"id": 323,
"slug": "178-1725288444-role-of-cd117-and-mast-cells-in-periodontal-diseases-histological-and-immunohistochemical-study",
"type": "original_article",
"manuscript_id": "178-1725288444",
"published": "2025-01-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/46/178-1725288444.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 182-191",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Khaphi FL, Hussein HA, et al. Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical study. J Adv Biotechnol Exp Ther. 2025; 8(1): 182-191.",
"title": "Role of CD117 and mast cells in periodontal diseases: Histological and immunohistochemical study",
"abstract": "<p>Mast cells are mobile secretory cells with a variety of biological tasks such as phagocytosis, processing of antigens, cytokine production, and the release of both newly formed and preexisting physiological mediators. This study aimed to investigate and establish the relationship between mast cells and various stages of periodontal diseases. A total of 36 soft tissue samples, 12 each of normal healthy periodontal, dental plaque periodontitis, and chronic periodontitis were selected. Samples were collected from patients with periodontal surgery in chronic periodontitis and plaque periodontitis. Samples were stained with 1% toluidine blue (TB), immunohistochemistry for CD117 expression, and observed under a light microscope for the presence of mast cells. All data were analyzed using SPSS. The mast cells were found to be highest in the chronic periodontitis group, followed by the dental plaque periodontitis group, as compared to the healthy periodontal group. The quantitative study of mast cells using TB staining showed statistically significant differences among the three groups. However, the results showed no statistically significant difference in the expression levels of CD117 in the plaque periodontitis and healthy periodontal group but showed a statistically significant difference between chronic periodontitis and healthy periodontal group. Thus, an increase in mast cells in human periodontal diseases suggests that these cells are crucial for developing chronic periodontitis.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.15",
"views": 391,
"downloads": 61,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/32/30/178-1725288444-Figure1.jpg",
"caption": "Figure 1. Images of clinical cases (A) healthy periodontal group, (B) plaque periodontitis, and (C) chronic periodontitis group.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/32/30/178-1725288444-Figure2.jpg",
"caption": "Figure 2. (A) Comparison of mean mast cell quantification among the study groups; and (B) The level of CD 117 expression among the study groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/32/30/178-1725288444-Figure3.jpg",
"caption": "Figure 3. Histological section of mast cells in: (A) healthy periodontal group, (B) plaque periodontitis group, and (C) chronic periodontitis group. TB stain, X 400.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/32/30/178-1725288444-Figure4.jpg",
"caption": "Figure 4. The mast cells contained in: (A) healthy periodontal group, (B) plaque periodontitis group, and (C) chronic periodontitis group (IHC, X400).",
"featured": true
}
],
"authors": [
{
"id": 1611,
"affiliation": [
{
"affiliation": "University of Basrah, College of Dentistry, Basrah city 61001, Iraq"
}
],
"first_name": "Fatin L.",
"family_name": "Khaphi",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 323
},
{
"id": 1612,
"affiliation": [
{
"affiliation": "University of Basrah, College of Dentistry, Basrah city 61001, Iraq"
}
],
"first_name": "Hanaa Ali",
"family_name": "Hussein",
"email": "hanaazahraa85@gmail.com",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-2848-5540",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Hanaa Ali Hussein, PhD\r\nUniversity of Basrah, College of Dentistry, Basrah city 61001, Iraq. Email: hanaazahraa85@gmail.com",
"article": 323
},
{
"id": 1613,
"affiliation": [
{
"affiliation": "University of Basrah, College of Dentistry, Basrah city 61001, Iraq"
}
],
"first_name": "Oula Fouad",
"family_name": "Hameed",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 323
},
{
"id": 1614,
"affiliation": [
{
"affiliation": "University of Basrah, College of Dentistry, Basrah city 61001, Iraq"
}
],
"first_name": "Huda Jassem",
"family_name": "Jebur",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 323
},
{
"id": 1615,
"affiliation": [
{
"affiliation": "University of Basrah, College of Dentistry, Basrah city 61001, Iraq"
}
],
"first_name": "Zahra Kadhum",
"family_name": "Saeed",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 323
}
]
},
{
"id": 322,
"slug": "178-1729709059-prevalence-and-consequences-of-bovine-subclinical-mastitis-in-hill-tract-areas-of-the-chattogram-division-bangladesh",
"type": "original_article",
"manuscript_id": "178-1729709059",
"published": "2025-01-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/08/178-1729709059.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 163-181",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Mia MP, Hossain H, et al. Prevalence and consequences of bovine subclinical mastitis in hill tract areas of the Chattogram division, Bangladesh. J Adv Biotechnol Exp Ther. 2025; 8(1): 163-181.",
"title": "Prevalence and consequences of bovine subclinical mastitis in hill tract areas of the Chattogram division, Bangladesh",
"abstract": "<p>Mastitis is a common problem in Bangladeshi dairy cattle production. This study investigated the prevalence of subclinical mastitis (SCM), its impact on milk production and reproduction, and the resultant economic losses among dairy farms in the Chattogram division, Bangladesh. A cross-sectional study evaluated 161 farms, collecting 4,048 quarter milk samples from 1,012 cows to conduct a primary screening for SCM using the California mastitis test (CMT). Additional surveys were conducted on SCM-positive farms and animals to explore production and economic impacts. The prevalence of SCM at the farm level was 76.8% (43/56) in Chattogram and 69.5% (73/105) in Cox’s Bazar. At the quarter level, the prevalence of SCM was found to be 32.29% (1307/4048; 95% CI: 30.9-33.8), while at the animal level, it was 41.3% (418/1012; 95% CI: 38.3-44.4). Crossbred cows exhibited a higher susceptibility to SCM at 47.2%, compared to indigenous breeds (31.2%). SCM significantly (<em>p</em><0.05) altered milk's physicochemical properties, reducing fat, protein, and mineral content and negatively affecting milk quality, and market value along with some key reproductive parameters, particularly in Crossbred and Holstein Friesian cows. The economic losses due to SCM are substantial, primarily driven by decreased milk production and increased treatment costs. Crossbred and Holstein Friesian cows suffer the most significant financial losses from their higher milk production and treatment expenses. In conclusion, this study provided valuable insights for policymakers, veterinarians, and farmers, to adopt effective SCM control measures to improve the productivity of the dairy industry in Bangladesh.</p>",
"DOI": "10.5455/jabet.2025.14",
"views": 553,
"downloads": 72,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/36/28/178-1729709059-Figure1.jpg",
"caption": "Figure 1. The study area map shows the selected district and sub-district of the experiment. The map was created using ArcMap 10.8.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/36/28/178-1729709059-Figure2.jpg",
"caption": "Figure 2. A) Frequency of SCM on CMT positive quarters. Ns: Non-significant, *P<0.05, **P<0.01, ***P<0.001. B) SCM positive results on the basis of CMT grading.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/36/28/178-1729709059-Figure3.jpg",
"caption": "Figure 3. Comparative assessment (Normal vs SCM milk) of milk due to the effects of SCM. A) Changes of physical properties of milk due to SCM compared with normal healthy milk. B) Changes of chemical properties of milk due to SCM. C) Minerals and albumin. D) Level of protein fractions. [ns: non-significant, *p<0.05, **p<0.01, ***p<0.001, Independent sample t-test]",
"featured": false
}
],
"authors": [
{
"id": 1597,
"affiliation": [
{
"affiliation": "Department of Dairy Science, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Piplu",
"family_name": "Mia",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-7048-8043",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1598,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Hemayet",
"family_name": "Hossain",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-9785-2549",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1599,
"affiliation": [
{
"affiliation": "Department of Zoology (GSSC), University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Khadiza Akter",
"family_name": "Brishty",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0001-9380-2579",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1600,
"affiliation": [
{
"affiliation": "Department of Medicine, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Shahidur Rahman",
"family_name": "Chowdhury",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0003-0210-6581",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1601,
"affiliation": [
{
"affiliation": "Department of Agriculture and Environmental Sciences; Lincoln University, MO 65101, USA"
}
],
"first_name": "Md.",
"family_name": "Imranuzzaman",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0002-3052-5727",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1602,
"affiliation": [
{
"affiliation": "Department of Dairy & Poultry Science; Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Mostafizor",
"family_name": "Rahman",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1603,
"affiliation": [
{
"affiliation": "Department of Chemistry (EMC); University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Sadia Islam",
"family_name": "Ria",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1604,
"affiliation": [
{
"affiliation": "Faculty of Veterinary Medicine; Chattogram Veterinary and Animal Sciences University, Chattogram-4202, Bangladesh"
}
],
"first_name": "Milon",
"family_name": "Mia",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1605,
"affiliation": [
{
"affiliation": "Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md Saidul",
"family_name": "Haque",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1606,
"affiliation": [
{
"affiliation": "Department of Dairy Science, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Ashik-Uz-",
"family_name": "Zaman",
"email": null,
"author_order": 10,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1607,
"affiliation": [
{
"affiliation": "Department of Genetics and Animal Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Nazmul",
"family_name": "Haque",
"email": null,
"author_order": 11,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1608,
"affiliation": [
{
"affiliation": "Department of Dairy Science, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Anzuman",
"family_name": "Ara",
"email": "anzuman.ds@sau.ac.bd",
"author_order": 12,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Anzuman Ara, Department of Dairy Science, Sylhet Agricultural University, Sylhet-3100, Bangladesh. Email: anzuman.ds@sau.ac.bd",
"article": 322
},
{
"id": 1609,
"affiliation": [
{
"affiliation": "Department of Pathology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Masudur",
"family_name": "Rahman",
"email": null,
"author_order": 13,
"ORCID": "https://orcid.org/0000-0001-7095-9303",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 322
},
{
"id": 1610,
"affiliation": [
{
"affiliation": "Department of Medicine, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Mahfujur",
"family_name": "Rahman",
"email": "mahfuj.vetmed@sau.ac.bd",
"author_order": 14,
"ORCID": "https://orcid.org/0000-0003-2062-8471",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Mahfujur Rahman, Department of Medicine, Sylhet Agricultural University, Sylhet-3100, Bangladesh.\r\nEmail: mahfuj.vetmed@sau.ac.bd",
"article": 322
}
]
},
{
"id": 321,
"slug": "178-1722874475-antiviral-and-anti-inflammatory-activities-of-favipiravir-and-quinine-sulfate-against-dengue-virus-serotype-1-in-vitro",
"type": "original_article",
"manuscript_id": "178-1722874475",
"published": "2025-01-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/36/178-1722874475.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 150-162",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Rosalina RA, Dewi BE, et al. Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro. J Adv Biotechnol Exp Ther. 2025; 8(1): 150-162.",
"title": "Antiviral and anti-inflammatory activities of favipiravir and quinine sulfate against dengue virus serotype 1 in vitro",
"abstract": "<p>Dengue virus (DENV) infection is still a global health problem. The severity of DENV infection is related to the high viral load and cytokine storm caused by excessive inflammation. There is no specific antiviral used for DENV. Meanwhile, the use of anti-inflammatory drugs for DENV is limited to patients with severe clinical symptoms. Interestingly, favipiravir (FVP) and quinine sulfate (QS) have been reported as repurposing drugs that can inhibit DENV replication. However, their anti-inflammatory activity in DENV infection has not been studied yet. Thus, the current study aimed to evaluate both antiviral and inflammatory activities of FVP and QS in Vero and PBMC cells. The FVP and QS antiviral activities were analyzed through half-maximal inhibitory concentration (IC<sub>50</sub>) and half-maximal cytotoxicity concentration (CC<sub>50</sub>) values against DENV serotype-1 on Vero cells. The anti-inflammatory activities of FVP and QS were measured by the relative expression of TNF-α, IL-6, IL-10 cytokines, and the transcription factor NF-κB from DENV-1 infected peripheral blood mononuclear cells (PBMC) in vitro. The results showed that the IC<sub>50</sub>, CC<sub>50</sub>, and selectivity index (SI) for FVP were 2.72 μg/mL, 156.78 μg/mL, and 58, respectively. Meanwhile, the IC<sub>50</sub>, CC<sub>50</sub>, and SI of QS were 14.97 μg/mL, 85.2 μg/mL, and 5.69. Also, FVP and QS reduced the expression of IL-6 and IL-10 but induced the expression of TNF-α, and the transcription factor NF-κB in PBMC with the presence of antibody-dependent enhancement (ADE). Further, FVP has better DENV-1 antiviral activities compared to QS. However, in comparison with QS, FVP showed lower anti-inflammatory activities. Further studies are needed to explore the antiviral and anti-inflammatory mechanism of FVP and QS in the DENV-infected models. <strong> </strong></p>",
"DOI": "10.5455/jabet.2025.13",
"views": 563,
"downloads": 83,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/09/27/178-1722874475-Figure1.jpg",
"caption": "Figure 1. DENV-1 focus assay results in Vero E6 C1008 cells treated with various concentrations of Favipiravir. 1 foci came from one infected DENV-1 that was replicated in Vero cells for 2 days of incubation. A. Negative Control (DMSO 0.1%), B. Favipiravir 10 μg/mL, C. Favipiravir 5 μg/mL, D. Favipiravir 2.5 μg/mL, E. Favipiravir 1.25 μg/mL, F. Favipiravir 0.625 μg/mL, G. Favipiravir 0.3125 μg/mL. Using 20x magnification and Focus Forming Assay (FFA).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/09/27/178-1722874475-Figure2.jpg",
"caption": "Figure 2. Linear regression graph between favipiravir concentration and percent inhibition. The R-value was 0.9448, indicating that experiments were in a dose-dependent manner.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/09/27/178-1722874475-Figure3.jpg",
"caption": "Figure 3. DENV-1 focus assay results in Vero E6 C1008 cells treated with various concentrations of Quinine Sulfate. One foci came from one infected DENV-1 that was replicated in Vero cells for 2 days of incubation. A. Negative Control (DMSO 0,1%), B. Quinine Sulfate 160 μg/mL, C. Quinine Sulfate 80 μg/mL, D. Quinine Sulfate 40 μg/mL, E. Quinine Sulfate 20 μg/mL, F. Quinine Sulfate 10 μg/mL, G. Quinine Sulfate 5 μg/mL. Using 20x magnification.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/09/27/178-1722874475-Figure4.jpg",
"caption": "Figure 4. Logarithmic regression graph between Quinine Sulfate concentration and percent inhibition. The R-value was 0.9313, indicating that experiments were in a dose-dependent manner.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/09/27/178-1722874475-Figure5.jpg",
"caption": "Figure 5. The percentage viability of Vero cells at various concentrations of favipiravir. The R-value was 0.9725, indicating that experiments were in a dose-dependent manner.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/09/27/178-1722874475-Figure6.jpg",
"caption": "Figure 6. The percentage viability of Vero cells at various concentrations of quinine sulfate. The R-value was 0.9775, indicating that experiments were in a dose-dependent manner.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/09/27/178-1722874475-Figure7.jpg",
"caption": "Figure 7. Relative mRNA expression of transcription factor A) NF-κB and cytokines B) TNF-α, C) IL-6, and D) IL10 after treatment with favipiravir and quinine sulfate in PBMC infected with DENV-1 in the presence of antibodies as ADE model. Each relative mRNA expression value was represented from a triplicate sample. The doses of FVP and QS used were 2xIC50, as determined by the antiviral activity test.",
"featured": false
}
],
"authors": [
{
"id": 1594,
"affiliation": [
{
"affiliation": "Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia"
}
],
"first_name": "Regita Aulia",
"family_name": "Rosalina",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0003-0445-6718",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 321
},
{
"id": 1595,
"affiliation": [
{
"affiliation": "Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10320, Indonesia"
},
{
"affiliation": "Infectious Disease and Immunology Research Center Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia"
}
],
"first_name": "Beti Ernawati",
"family_name": "Dewi",
"email": "beti.ernawati@ui.ac.id",
"author_order": 2,
"ORCID": "https://orcid.org/0009-0002-0452-1914",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Beti Ernawati Dewi, Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10320, Indonesia. Email: beti.ernawati@ui.ac.id",
"article": 321
},
{
"id": 1596,
"affiliation": [
{
"affiliation": "Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10320, Indonesia"
}
],
"first_name": "Fithriyah",
"family_name": null,
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 321
}
]
},
{
"id": 320,
"slug": "178-1730138656-trajectory-of-human-migration-insights-from-autosomal-and-non-autosomal-variant-clustering-patterns",
"type": "short_communication",
"manuscript_id": "178-1730138656",
"published": "2025-01-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/31/178-1730138656.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 139-149",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Bangladesh",
"cite_info": "Tuli SS, Sarker J, et al. Trajectory of human migration: Insights from autosomal and non-autosomal variant clustering patterns. J Adv Biotechnol Exp Ther. 2025; 8(1): 139-149.",
"title": "Trajectory of human migration: Insights from autosomal and non-autosomal variant clustering patterns",
"abstract": "<p>Genetic variations present in the Y chromosomal and mitochondrial DNA provide the molecular basis to support the archeological and anthropological evidence that formulates the theories for describing the trajectory of human migration, which started almost 200,000 years ago out of Africa. These genetic variations have long been used as ancestry informative markers (AIMs) in forensics and evolutionary studies, primarily because of their uniparental inheritance and lack of recombination, despite the fact that gender specific gene flow and socio-cultural practices may cause discrepancies. Moreover, the genetic markers on the Y- chromosome constitute only a minor fraction of the entire human genome. Here, we analyzed over 75 million genetic variants (single nucleotide variants (SNVs) and short insertion-deletion (InDels)) within consecutive 2500000 base pair windows in the autosomal as well as non-autosomal chromosomes of 22 populations in four major geographic regions that are cataloged in the 1000 Genomes Project to understand the clustering patterns of the autosomal and non-autosomal variants. While autosomal and X-chromosomal variants cluster the populations of similar geographic regions together, Y-chromosomal variants constantly place the East Asian Japanese, and the European Finnish populations in a single clade in hierarchical clusters. In conclusion, this comprehensive genome-wide analysis essentially introduces new insights into mapping the path of human migration based on the Y-chromosomal and other chromosomal variants.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.12",
"views": 637,
"downloads": 81,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/06/26/178-1730138656-Figure1.jpg",
"caption": "Figure 1. Clustering of genetic variants in four super-populations in PCA plot (A-I). Randomly selected plots are shown. Chromosome number and genetic regions are shown in each plot (e.g., “1: 47500001-50000000” indicates base positions between 47500001 and 50000000 in human chromosome 1). AFR- African, EAS- East Asian, EUR- European, SAS- South Asian.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/06/26/178-1730138656-Figure2.jpg",
"caption": "Figure 2. Hierarchical clustering of autosomal and X chromosomal variants (A-I). Chromosome number and genetic regions are shown in each plot (e.g., “1: 1-2500000” indicates base positions between 1 and 2500000 in human chromosome 1). African super-population (AFR)- ((Yoruba in Ibadan, Nigeria (YRI); Luhya in Webuye, Kenya (LWK); Gambian in Western Divisions in the Gam-bia (GWD); Mende in Sierra Leone (MSL); Esan in Nigeria (ESN); Americans of African Ancestry in SW USA (ASW); African Caribbeans in Barbados (ACB)); East Asian (EAS)- ((Han Chinese in Beijing, China (CHB); Japanese in Tokyo, Japan (JPT); Southern Han Chinese (CHS); Chinese Dai in Xishuangbanna, China (CDX); Kinh in Ho Chi Minh City, Vietnam (KHV)); European (EUR)- ((Utah Residents (CEPH) with Northern and Western European Ancestry (CEU); Toscani in Italia (TSI); Finnish in Finland (FIN); British in England and Scotland (GBR); Iberian Population in Spain (IBS)); South Asian (SAS)- ((Gujarati Indian from Houston, Texas (GIH); Punjabi from Lahore, Pakistan (PJL); Bengali from Bangladesh (BEB); Sri Lankan Tamil from the UK (STU); Indian Telugu from the UK (ITU)).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/06/26/178-1730138656-Figure3.jpg",
"caption": "Figure 3. Clustering of Y chromosomal variants in PCA plot (A-I). Randomly selected plots are shown. Chromosome number and genetic regions are shown in each plot (e.g., “Y: 2500001-5000000” indicates base positions between 2500001 and 5000000 in human chromosome Y). AFR- African, EAS- East Asian, EUR- European, SAS- South Asian.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/06/26/178-1730138656-Figure4.jpg",
"caption": "Figure 4. Hierarchical clustering of Y chromosomal variants (A-I). Chromosome number and genetic regions are shown in each plot (e.g., “Y: 12500001-15000000” indicates base positions between 12500001 and 15000000 in human chromosome Y). African super-population (AFR)- ((Yoruba in Ibadan, Nigeria (YRI); Luhya in Webuye, Kenya (LWK); Gambian in Western Divisions in the Gambia (GWD); Mende in Sierra Leone (MSL); Esan in Nigeria (ESN); Americans of African Ancestry in SW USA (ASW); African Caribbeans in Barbados (ACB)); East Asian (EAS)- ((Han Chinese in Beijing, China (CHB); Japanese in Tokyo, Japan (JPT); Southern Han Chinese (CHS); Chinese Dai in Xishuangbanna, China (CDX); Kinh in Ho Chi Minh City, Vietnam (KHV)); European (EUR)- ((Utah Residents (CEPH) with Northern and Western European Ancestry (CEU); Toscani in Italia (TSI); Finnish in Finland (FIN); British in England and Scotland (GBR); Iberian Population in Spain (IBS)); South Asian (SAS)- ((Gujarati Indian from Houston, Texas (GIH); Punjabi from Lahore, Pakistan (PJL); Bengali from Bangladesh (BEB); Sri Lankan Tamil from the UK (STU); Indian Telugu from the UK (ITU)).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/06/26/178-1730138656-Figure5.jpg",
"caption": "Figure 5. Clustering of variants located near the centromeric regions in PCA plot (A-I). Randomly selected plots are shown. Chromosome number and genetic regions are shown in each plot (e.g., “1: 120000001-122500000” indicates base positions between 120000001 and 122500000 in human chromosome 1). AFR- African, EAS- East Asian, EUR- European, SAS- South Asian.",
"featured": false
}
],
"authors": [
{
"id": 1586,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Samayeta Sarkar",
"family_name": "Tuli",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 320
},
{
"id": 1587,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Joyatry",
"family_name": "Sarker",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 320
},
{
"id": 1588,
"affiliation": [
{
"affiliation": "Department of Computer Science and Engineering, Southeast University, Dhaka, Bangladesh"
},
{
"affiliation": "University of Illinois Urbana-Champaign, Urbana, IL-61820, USA"
}
],
"first_name": "Mrinmoy Saha",
"family_name": "Roddur",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 320
},
{
"id": 1589,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Anik",
"family_name": "Biswas",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 320
},
{
"id": 1590,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Reefa",
"family_name": "Nawar",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 320
},
{
"id": 1591,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Tahmina",
"family_name": "Akter",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 320
},
{
"id": 1592,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Md. Wahid",
"family_name": "Murad",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 320
},
{
"id": 1593,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Abu Ashfaqur",
"family_name": "Sajib",
"email": "abu.sajib@du.ac.bd",
"author_order": 8,
"ORCID": "https://orcid.org/0000-0003-1710-9865",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Abu Ashfaqur Sajib , Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh. Email: abu.sajib@du.ac.bd",
"article": 320
}
]
},
{
"id": 319,
"slug": "178-1734634003-pharmacological-insights-and-therapeutic-advances-of-mangiferin-against-various-pathological-conditions-in-liver-diseases",
"type": "review_article",
"manuscript_id": "178-1734634003",
"published": "2025-01-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2025/28/178-1734634003.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 125-138",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Shoyshob TZ, Akter S, et al. Pharmacological insights and therapeutic advances of mangiferin against various pathological conditions in liver diseases. J Adv Biotechnol Exp Ther. 2025; 8(1): 125-138.",
"title": "Pharmacological insights and therapeutic advances of mangiferin against various pathological conditions in liver diseases",
"abstract": "<p>Currently, liver diseases have become a serious global health issue, and this situation occurs due to the exposure of the liver to different agents, such as chemicals, alcohol, viruses, and autoimmune diseases. Mangiferin is a natural bioactive xanthone C-glycoside compound and a potent antioxidant that is widely found in medicinal plants such as the leaves of <em>Mangifera indica L</em>. (<em>Anacardiaceae</em>). It possesses a wide range of biological properties, such as antidiabetic, hepatoprotective, anti-inflammatory, antioxidant, and anticarcinogenic activities. The purpose of this literature review was to delineate the hepatoprotective effects of mangiferin, a natural bioactive compound without side effects, and explain how it protects the liver via the suppression of pathological conditions involved in liver diseases. Relevant published research articles from peer-reviewed journals were searched in PubMed and Google Scholar to gain insights into the consequences of mangiferin in liver diseases. Several studies have suggested that pretreatment with mangiferin decreases hepatic inflammation, oxidative stress, apoptosis, fibrosis, endoplasmic reticulum (ER) stress, and hepatic dysfunction and concomitantly ameliorates the morphological structures of the liver. Therefore, mangiferin could be considered a multitarget therapeutic and promising drug candidate for the treatment of hepatic diseases, although a detailed mechanistic explanation needs to be provided. This literature review highlights the pathological conditions (inflammation, oxidative stress, apoptosis, ER stress) associated with liver diseases as well as the hepatoprotective and therapeutic effects of mangiferin in the liver.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.11",
"views": 649,
"downloads": 106,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/58/21/178-1734634003-Figure1.jpg",
"caption": "Figure 1. Chemical structure of mangiferin (C19H18O11) [19].",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2025/58/21/178-1734634003-Figure2.jpg",
"caption": "Figure 2. Summary of the protective mechanisms of mangiferin against liver disease. Stress stimuli such as HFD, D-GalN, STZ, LPS, As, and ischemia trigger the generation of ROS, which reduce the levels of several antioxidant enzymes (SOD, CAT, GST, GPx) and hence cause oxidative stress, ultimately resulting in liver diseases. Mangiferin intercepts oxidative stress by increasing the level of antioxidant enzymes. In addition, these stimuli also trigger inflammatory cascades via the activation of the NF-қB pathway. Nevertheless, mangiferin blocks inflammatory cascades via the suppression of ROS and the NF-қB pathway. The production of cytochrome C decreases the expression of Bcl-2, thus decreasing apoptosis. Mangiferin also inhibits ER stress by suppressing CHOP and GRP78. Furthermore, after the activation of TNF-α, it helps to activate caspase compounds, resulting in apoptosis, and mangiferin inhibits this apoptosis in the liver.",
"featured": true
}
],
"authors": [
{
"id": 1578,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "Tanvir Zaman",
"family_name": "Shoyshob",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-8853-9769",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 319
},
{
"id": 1579,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "Sumaya",
"family_name": "Akter",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 319
},
{
"id": 1580,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "Muhammad Ramiz",
"family_name": "Uddin",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 319
},
{
"id": 1581,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "Mithila",
"family_name": "Farjana",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 319
},
{
"id": 1582,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "Akhi",
"family_name": "Moni",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0002-5812-7755",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 319
},
{
"id": 1583,
"affiliation": [
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of Korea"
}
],
"first_name": "Wonhyo",
"family_name": "Seo",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 319
},
{
"id": 1584,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md. Golzar",
"family_name": "Hossain",
"email": null,
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-1487-5444",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 319
},
{
"id": 1585,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of Korea"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": "hasan800920@gmail.com",
"author_order": 8,
"ORCID": "https://orcid.org/0000-0003-2911-3255",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh. Email: hasan800920@gmail.com",
"article": 319
}
]
},
{
"id": 318,
"slug": "178-1719701008-investigation-on-the-effectiveness-of-progranulin-as-a-novel-predictive-biomarker-for-allergic-disorders",
"type": "original_article",
"manuscript_id": "178-1719701008",
"published": "2024-12-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/38/178-1719701008.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 116-124",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Mustafa NW, Elia ZN, et al. Investigation on the effectiveness of progranulin as a novel predictive biomarker for allergic disorders. J Adv Biotechnol Exp Ther. 2025; 8(1): 116-124.",
"title": "Investigation on the effectiveness of progranulin as a novel predictive biomarker for allergic disorders",
"abstract": "<p>Progranulin (PGRN) has been implicated in the emergence of several inflammatory conditions. However, the function of PGRN in allergy-related conditions has not been clearly elucidated. Thus, the current study aimed to investigate the effectiveness of PGRN as a biomarker in allergic disorders. A total of 124 participants (84 with allergic disorders and 40 healthy individuals) were included in this study. Patients were divided into two groups, 38 were classified as having allergies related to the respiratory system and 46 with allergies related to the skin, based on their clinical diagnosis. This categorization was confirmed by serological diagnosis by estimating the levels of immunoglobulin E (IgE). Additionally, C-reactive protein (CRP) levels were measured for the detection of inflammation. Serum PGRN level was estimated by the enzyme-linked immunosorbent assay (ELISA) technique, while interleukin-6 (IL-6) concentration was determined by the fluorescent immunoassay technique. Serum PGRN levels were significantly increased (p>0.05) in all patient groups as compared with controls. Adult patients recorded a significant elevation in PGRN level (p<0.05) compared to the children, while no significant difference (p>0.05) was recorded in terms of disease type (respiratory tract and skin allergies) or gender. Additionally, the results of Spearman’s correlation test revealed that there was no significant correlation between PGRN and each of IgE, CRP, and IL-6. Thus, the current study concluded that PGRN had efficacy as a predictive biomarker for various allergic disorders.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.10",
"views": 599,
"downloads": 96,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/53/24/178-1719701008-Figure1.jpg",
"caption": "Figure 1. Comparison of serum PGRN levels (ng/ml) among patient groups and control. Serum PGRN levels were significantly elevated (****p-value ≤ 0.0001) in patients with allergies compared with controls. (A) total patients. (B) Patients with respiratory allergic conditions. (C) Patients with skin allergic conditions. (D) Patients with normal CRP levels. (E) Patients with elevated CRP levels. PGRN, progranulin; CRP, C-reactive protein.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/53/24/178-1719701008-Figure2.jpg",
"caption": "Figure 2. Correlation between PGRN levels and biomarkers of allergies and inflammations. PGRN levels exhibited no correlations with A) IgE levels (r = 0.09374; p < 0.586), B) CRP levels (r = -0.2281; p < 0.472), and C) IL-6 levels (r = 0.2437; p < 0.300) in patients with allergic disorders. PGRN: Progranulin, IgE: Immunoglobulin E, CRP: C-Reactive protein, IL-6: Interleukin-6.",
"featured": false
}
],
"authors": [
{
"id": 1572,
"affiliation": [
{
"affiliation": "Department of Clinical Pharmacy, College of Pharmacy, University of Basrah, Iraq"
}
],
"first_name": "Nisreen W.",
"family_name": "Mustafa",
"email": "nisrenw@yahoo.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-9477-9371",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nisreen W. Mustafa, Department of Clinical Pharmacy, College of Pharmacy, University of Basrah, Iraq. Email: nisrenw@yahoo.com",
"article": 318
},
{
"id": 1573,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Zaid N.",
"family_name": "Elia",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 318
},
{
"id": 1574,
"affiliation": [
{
"affiliation": "Pharmaceutical Department, Pharmacy College, Ninevah University, Iraq"
}
],
"first_name": "Abeer M. Abdel",
"family_name": "Rasool",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 318
},
{
"id": 1575,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Sanaria F.",
"family_name": "Jarjes",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 318
},
{
"id": 1576,
"affiliation": [
{
"affiliation": "Department of Medicine, College of Medicine, University of Mosul, Iraq"
}
],
"first_name": "Salam F.",
"family_name": "Nawara",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 318
},
{
"id": 1577,
"affiliation": [
{
"affiliation": "Erbil Dermatologic Teaching Center, Iraq"
}
],
"first_name": "Shakhawan H.",
"family_name": "Hamid",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 318
}
]
},
{
"id": 317,
"slug": "178-1728556935-assessment-of-aluminium-level-in-commercial-pasteurized-and-uht-milk-in-bangladesh-and-their-potential-health-risks",
"type": "short_communication",
"manuscript_id": "178-1728556935",
"published": "2024-12-21",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/19/178-1728556935.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 105-115",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Jahan I, Al-Noman KM, et al. Assessment of aluminium level in commercial pasteurized and UHT milk in Bangladesh and their potential health risks. J Adv Biotechnol Exp Ther. 2025; 8(1): 105-115.",
"title": "Assessment of aluminium level in commercial pasteurized and UHT milk in Bangladesh and their potential health risks",
"abstract": "<p>Aluminium (Al) contamination in the dairy industry is an emerging concern due to potential health risks. With the increasing consumption of pasteurized and UHT milk in Bangladesh, monitoring Al levels has become crucial. This study aimed to measure Al concentrations in these processed products using inductively coupled plasma mass spectrometry (ICP-MS) and assess the associated health risks. Milk samples from 10 brands, including three different production lots, were collected from supermarkets. The instrumental precision for Al was 0.0212 mg/kg as a limit of detection (LOD) and 0.0643 mg/kg as a limit of quantification (LOQ), with a relative standard deviation (RSD) of 3.2%. The multi-element standard containing Al exhibited linearity in the concentration ranged from 0-0.5 mg/kg with a correlation coefficient value of 0.9994. Among the 30 samples analyzed, 13, including both pasteurized and UHT milks, were contaminated with Al. The mean Al concentrations in the contaminated samples were 0.46±0.5, 0.71±0.6, and 0.47±0.4 mg/kg in lots 1, 2, and 3, respectively, indicating variation across production lots. The estimated daily Al intake ranged from 2.00E-03 to 3.00E-03 mg/kg body weight, contributing 1.4 to 2.1% of the provisional tolerable weekly intake. The target hazard quotient (THQ) for the contaminated samples ranged from 0.045 to 0.070 (THQ < 1), suggesting that the Al levels pose no significant health risk. Thus, the Al content in pasteurized and UHT milk may be considered safe for consumption, albeit with the limitation of a small sample size in this study.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.09",
"views": 605,
"downloads": 94,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/45/21/178-1728556935-Figure1.jpg",
"caption": "Figure 1. Calibration curve of Al using multi-element standard solution at six various concentrations (0, 1, 20, 100, 300, 500 µg/l).",
"featured": true
}
],
"authors": [
{
"id": 1565,
"affiliation": [
{
"affiliation": "Department of Dairy and Poultry Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
},
{
"affiliation": "Department of Dairy and Poultry Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh"
}
],
"first_name": "Ishrat",
"family_name": "Jahan",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0000-7742-4467",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 317
},
{
"id": 1566,
"affiliation": [
{
"affiliation": "Department of Dairy and Poultry Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
},
{
"affiliation": "Department of Animal Production, Gono Bishwabidyalay, Dhaka-1344, Bangladesh"
}
],
"first_name": "Kazi Md.",
"family_name": "Al-Noman",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-2001-6232",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 317
},
{
"id": 1567,
"affiliation": [
{
"affiliation": "Department of Dairy and Poultry Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Shabbir",
"family_name": "Ahmed",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-2209-3837",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 317
},
{
"id": 1568,
"affiliation": [
{
"affiliation": "Department of Dairy and Poultry Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Mst. Umme",
"family_name": "Habiba",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-7856-1831",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 317
},
{
"id": 1569,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "S. A. Masudul",
"family_name": "Hoque",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0003-3537-5892",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 317
},
{
"id": 1570,
"affiliation": [
{
"affiliation": "Department of Animal Science and Nutrition, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Abu Sadeque Md.",
"family_name": "Selim",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0001-8457-4631",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 317
},
{
"id": 1571,
"affiliation": [
{
"affiliation": "Department of Dairy and Poultry Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
},
{
"affiliation": "Institute of Food Safety and Processing, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Md. Morshedur",
"family_name": "Rahman",
"email": "morshed@bsmrau.edu.bd",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-6474-8902",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Morshedur Rahman, PhD; Department of Dairy and Poultry Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706, Bangladesh. Email: morshed@bsmrau.edu.bd",
"article": 317
}
]
},
{
"id": 316,
"slug": "178-1715929158-cloning-expression-and-purification-of-fusion-antigens-mpt83-and-esat6-from-the-local-strain-of-mycobacterium-tuberculosis-in-escherichia-coli-as-a-seed-vaccine-candidate-against-tuberculosis",
"type": "original_article",
"manuscript_id": "178-1715929158",
"published": "2024-12-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/11/178-1715929158.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 91-104",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Cloning, expression, and purification of fusion antigens MPT83 and ESAT6 from the local strain of Mycobacterium tuberculosis in Escherichia coli as a seed vaccine candidate against tuberculosis. J Adv Biotechnol Exp Ther. 2025; 8(1): 91-104",
"title": "Cloning, expression, and purification of fusion antigens MPT83 and ESAT6 from the local strain of <span>Mycobacterium tuberculosis</span> in <span>Escherichia coli</span> as a seed vaccine candidate against tuberculosis",
"abstract": "<p>Eradicating tuberculosis (TB) globally is increasingly challenging with the growing number of drug-resistant <em>Mycobacterium tuberculosis</em> (<em>M. tuberculosis</em>) strains. The development of more potent TB vaccines is critical to complement overall TB control strategies and overcome the growing challenge of drug resistance. In this study, the recombinant plasmid pGEM-T Easy- Rv2873 + Rv3875 has been generated by inserting the Rv3875 gene, which encodes the ESAT6 protein, into the pGEM-T Easy- Rv2873 vector at the <em>BamH</em>I/<em>Hind</em>III cloning site. Following transformation into <em>E. coli</em> JM109, the plasmid was extracted, PCR amplified, and DNA sequencing. The existence of the appropriate recombinant Rv2873 + Rv3875 fusion genes was confirmed through the observation of a band of 948 base pairs in the colony PCR product containing fusion antigens. A band measuring 3966 base pairs was observed in the recombinant plasmid pGEM-T Easy- Rv2873+Rv3875, supporting the presence of the desired fusion genes target. The fusion genes Rv2873+Rv3875 were cloned into the expression vector pTrcHisA. This resulted in the pTrcHisA Rv2873+Rv3875 recombinant fusion plasmid, which was subsequently introduced into the <em>E. coli</em> BL21 strain through transformation. The fusion protein, comprising the 6XHis tag, exhibited a molecular mass of around 28 kilo Dalton and was synthesized as an insoluble protein inside <em>E. coli </em>BL21. In conclusion, the purified recombinant fusion protein MPT83 and ESAT6 hold promise for TB diagnosis and show potential as vaccine candidates in the future.</p>",
"DOI": "10.5455/jabet.2025.08",
"views": 592,
"downloads": 104,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/19/178-1715929158-Figure1.jpg",
"caption": "Figure 1. A) DNA amplification by polymerase chain reaction visualized on a 1% agarose gel, and B) Purification of PCR-amplified DNA encoding the ESAT6 protein from the Rv3875 gene using a Purification Kit (Qiagen).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/19/178-1715929158-Figure2.jpg",
"caption": "Figure 2. E. coli recombinant white colony transformation and screening (- control), there is no growth in plasmid-free competent cells of the E. coli strain JM109 (A). Blue colony, which harbors the vector pGEM-T Easy alone as control of transformation (B). Many white colonies, which harbor the vector pGEM-T Easy- Rv2873, were seen following the transformation of E. coli JM109 competent cells (C). The recombinant plasmid pGEM-T Easy- Rv2873 +Rv3875 is responsible for the proliferation of white colonies in E. coli cells (D).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/19/178-1715929158-Figure3.jpg",
"caption": "Figure 3. After running the Rv3875 gene via an agarose gel electrophoresis, the gene is ligated into the pGEM-T Easy- Rv2873 vector. Column 1: BamHI and HindIII enzymes can only cut the pGEM-T Easy- Rv2873 vector to produce one DNA band with size 3678 bps. Column 2: Two DNA bands of 3678 and 288 base pairs are produced from the BamHI / HindIII-restricted product of pGEM-T Easy- Rv2873 +Rv3875 vector/plasmid—specifically, 100 bps in Column M Genetic identifier.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/19/178-1715929158-Figure4.jpg",
"caption": "Figure 4. (A) The PCR product of the fusion genes Rv2873 and Rv3875 codes for MPT83 and ESAT6 fusion proteins. Columns 1-2 show the PCR results for the white colonies from two independent clones, indicating that their PCR product had a length of 948 bps, whereas column 3 served as a negative control. Isolated white colonies were inoculated onto a PCR kit mix and subjected to the PCR procedure before being analyzed on an agarose gel electrophoresis device at 1.5 percent. (B) Mini-preparations of recombinant plasmid pGEM-T Easy-Rv2873 +Rv3875 (3966 bps in length) from three separate and independent white bacterial colonies on LB plate in Figure 2D.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/19/178-1715929158-Figure5.jpg",
"caption": "Figure 5. The predicted polynucleotide (cDNA) and amino acid (AA) sequences for the fusion proteins MPT83 and ESAT6 were encoded by the Rv2873 and Rv3875 genes, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/19/178-1715929158-Figure6.jpg",
"caption": "Figure 6. The recombinant plasmid pTrcHisA-Rv2873 +Rv3875 was introduced into the E. coli BL-21 strain. Subsequently, the transformed strain was evaluated on Luria-Bertani agar plates (A) and liquid culture media to express recombinant proteins with and without IPTG (B). Recombinant protein expression and growth of MPT83 plus ESAT6 in the absence and presence of 40 micromolar IPTG on SDS-PAGE analysis (C).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/19/178-1715929158-Figure7.jpg",
"caption": "Figure 7. Expression, purification, and SDS-PAGE (10%) analysis of purified recombinant MPT83 plus ESAT6 fusion proteins. It was possible to separate and purify 6XHis-tagged MPT83 and ESAT6 using affinity chromatography with a His Tag-agarose matrix. Proteins were isolated from cell lysates and various chromatographic fractions by electrophoresis in a 10% SDS-PAGE. The proteins could be seen thanks to CBB staining. Molecular weight proteins marker (M), induced whole cell lysates (Lane 1), un-induced whole cell lysates (Lane 2), wash flowthrough (Lane 3), flowthrough of elution with 0.5 M Tris-HCl buffer with 100 mM imidazole pH 7.3 (Lane 4), 200 mM imidazole pH 5.9 (Lane 5), and 300 mM imidazole pH 4.5 (Lane 6), MPT83 plus ESAT6 fusion proteins bands at 28 kDa are shown.",
"featured": true
}
],
"authors": [
{
"id": 1555,
"affiliation": [
{
"affiliation": "Medical Research Center, Faculty of Medicine, University of Hasanuddin, Makassar 90245, Indonesia"
},
{
"affiliation": "Department of Pharmacology, Faculty of Medicine, University of Hasanuddin, Makassar 90245, Indonesia"
}
],
"first_name": "Rusdina bte.",
"family_name": "Ladju",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-5393-0429",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 316
},
{
"id": 1556,
"affiliation": [
{
"affiliation": "Department of Chemistry, University of Hasanuddin, Makassar 90245, Indonesia"
},
{
"affiliation": "Puslitbang Biopolimer dan Bioproduk, Institute for Research and Community Services, University of Hasanuddin, Makassar 90245, Indonesia"
}
],
"first_name": "Ahyar",
"family_name": "Ahmad",
"email": "ahyarahmad@gmail.com",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-0913-4721",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ahyar Ahmad; Department of Chemistry, University of Hasanuddin, Makassar 90245, Indonesia. Email: ahyarahmad@gmail.com",
"article": 316
},
{
"id": 1557,
"affiliation": [
{
"affiliation": "Department of Chemistry, University of Hasanuddin, Makassar 90245, Indonesia"
}
],
"first_name": "Abdul",
"family_name": "Karim",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-1428-5559",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 316
},
{
"id": 1558,
"affiliation": [
{
"affiliation": "Department of Chemistry, University of Hasanuddin, Makassar 90245, Indonesia"
}
],
"first_name": "Rugaiyah",
"family_name": "Arfah",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0009-0009-4596-3349",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 316
},
{
"id": 1559,
"affiliation": [
{
"affiliation": "Department of Biology, University of Hasanuddin, Makassar 90245, Indonesia"
}
],
"first_name": "Rosana",
"family_name": "Agus",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-9418-4124",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 316
},
{
"id": 1560,
"affiliation": [
{
"affiliation": "Institute for Research and Community Services, University of Hasanuddin, Makassar 90245, Indonesia"
}
],
"first_name": "Najdah",
"family_name": "Hidayah",
"email": null,
"author_order": 6,
"ORCID": "http://orcid.org/0000-0002-9811-2080",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 316
},
{
"id": 1561,
"affiliation": [
{
"affiliation": "Institute for Research and Community Services, University of Hasanuddin, Makassar 90245, Indonesia"
},
{
"affiliation": "Department of Microbiology, University of Hasanuddin, Makassar 90245, Indonesia"
}
],
"first_name": "Muhammad Nasrum",
"family_name": "Massi",
"email": null,
"author_order": 7,
"ORCID": "http://orcid.org/0000-0002-3347-6529",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 316
},
{
"id": 1562,
"affiliation": [
{
"affiliation": "Research Center for Vaccine and Drugs, National Research and Innovation Agency, Banten, 15314, Indonesia"
}
],
"first_name": "Astutiati",
"family_name": "Nurhasanah",
"email": null,
"author_order": 8,
"ORCID": "http://orcid.org/0000-0003-0912-6913",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 316
},
{
"id": 1563,
"affiliation": [
{
"affiliation": "Department of Pharmacy, School of Pharmacy Yamasi, Makassar 90222, Indonesia"
}
],
"first_name": "Harningsih",
"family_name": "Karim",
"email": null,
"author_order": 9,
"ORCID": "http://orcid.org/0000-0002-1428-5559",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 316
},
{
"id": 1564,
"affiliation": [
{
"affiliation": "Clinical Pathology Laboratory, RSWS Hospital, Makassar 90245, Indonesia"
}
],
"first_name": "Irda",
"family_name": "Handayani",
"email": null,
"author_order": 10,
"ORCID": "http://orcid.org/0000-0002-6164-717X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 316
}
]
},
{
"id": 315,
"slug": "178-1715984206-evaluation-of-adipocytokines-and-oxidative-stress-biomarkers-in-sera-of-hemodialysis-patients",
"type": "original_article",
"manuscript_id": "178-1715984206",
"published": "2024-12-12",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/04/178-1715984206.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 79-90",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Evaluation of adipocytokines and oxidative stress biomarkers in sera of hemodialysis patients. J Adv Biotechnol Exp Ther. 2025; 8(1): 79-90",
"title": "Evaluation of adipocytokines and oxidative stress biomarkers in sera of hemodialysis patients",
"abstract": "<p>Chronic renal failure is a condition characterized by the inability of the kidney to effectively eliminate toxins, solutes, and excess water, necessitating the use of hemodialysis. The current study aimed to evaluate adipocytokines and oxidative stress biomarkers in sera of hemodialysis patients. This study involved 60 hemodialysis patients, of whom 64% were female and 36% were male. Additionally, 40 healthy control volunteers participated in the study. Adipocytokines and oxidative stress markers were measured using the ELISA technique, whereas the spectrophotometric method measured lipid profile and renal function. The t-test, correlation, receiver operating characteristic, and logistic regression were used in the statistical analysis. Patients undergoing hemodialysis exhibited significant differences compared to healthy controls in serum concentrations of several parameters. Significantly higher levels of adiponectin (23.46 ng/mL), resistin (5.50 ng/mL), visfatin (47.0 ng/mL), and chemerin (169.0 ng/mL) were measured in patients undergoing hemodialysis as compared to healthy controls. Similarly, significantly higher levels of oxidative stress markers such as superoxide dismutase (3.78 U/mL), glutathione peroxidase (129.79 pg/mL), and malondialdehyde (4.66 mmol/L) were measured in patients undergoing hemodialysis as compared to healthy controls. In conclusion, adipocytokine parameters and oxidative stress markers have been found to be abnormal in hemodialysis patients and have an effect on atherosclerosis and heart failure progression.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.07",
"views": 640,
"downloads": 95,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/19/178-1715984206-Figure1.jpg",
"caption": "Figure 1. Serum oxidative stress parameters concentrations such as (A) levels of superoxide dismutase (SOD) in HD patients and controls, (B) levels of glutathione peroxidase (GPx) in HD patients and controls, and (C) levels of malondialdehyde (MDA) in HD patients and controls. S indicates P < 0.05.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/19/178-1715984206-Figure2.jpg",
"caption": "Figure 2. Serum adipocytokines profile concentrations such as (A) levels of adiponectin in HD patients and controls, (B) levels of resistin in HD patients and controls, (C) levels of visfatin in HD patients and controls, and (D) levels of chemerin in HD patients and controls. S indicates P < 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/19/178-1715984206-Figure3.jpg",
"caption": "Figure 3. ROC curve of adipocytokines profile for the calculation of possible diagnostic cut-off value (A) Adiponectin ROC curve with cut-off value > 16.79, (B) resistin ROC curve with a cut-off value > 2.60, (C) visfatin ROC curve with cut-off value > 32.5, and (D) Chemerin ROC curve with cut-off value > 147.50.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/19/178-1715984206-Figure4.jpg",
"caption": "Figure 4. Logistic regression correlations between adipocytokines profile. A) Correlation between adiponectin and resistin, B) correlation between adiponectin and visfatin, C) correlation between adiponectin and chemerin, D) correlation between resistin and chemerin, E) correlation between resistin and visfatin, and F) correlation between chemerin and visfatin.",
"featured": false
}
],
"authors": [
{
"id": 1552,
"affiliation": [
{
"affiliation": "University of Baghdad, Department of Chemistry, College of Sciences, Baghdad, Iraq"
}
],
"first_name": "Shahad F.",
"family_name": "Obeid",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-7180-4335",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 315
},
{
"id": 1553,
"affiliation": [
{
"affiliation": "Mustansiriyah University, College of Basic Education, Baghdad, Iraq"
}
],
"first_name": "Israa Abass",
"family_name": "Rashed",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0009-0000-3767-5456",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 315
},
{
"id": 1554,
"affiliation": [
{
"affiliation": "Department of Applied Sciences, University of Technology- Iraq, Baghdad, Iraq"
}
],
"first_name": "Mohammed S.",
"family_name": "Al-Hindawi",
"email": "Mohammed.S.AlHindawi@uotechnology.edu.iq",
"author_order": 3,
"ORCID": "http://orcid.org/0000-0003-1487-5943",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammed S. Al-Hindawi, Department of Applied Sciences, University of Technology- Iraq, Baghdad, Iraq\r\nEmail: Mohammed.S.AlHindawi@uotechnology.edu.iq",
"article": 315
}
]
},
{
"id": 314,
"slug": "178-1726215483-genomic-insights-into-multidrug-resistant-acinetobacter-baumannii-strains-isolated-from-dhaka-bangladesh",
"type": "original_article",
"manuscript_id": "178-1726215483",
"published": "2024-12-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/03/178-1726215483.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 64-78",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Ferdous S, Shayer KMSA, et al., Genomic insights into multidrug-resistant Acinetobacter baumannii strains isolated from Dhaka, Bangladesh. J Adv Biotechnol Exp Ther. 2025 Jan; 8(1): 64-78.",
"title": "Genomic insights into multidrug-resistant <span>Acinetobacter baumannii</span> strains isolated from Dhaka, Bangladesh",
"abstract": "<p><em>Acinetobacter baumannii</em> is one of the most common nosocomial infections causing pathogens. Multidrug-resistant (MDR) <em>A. baumannii</em> is a growing concern to the healthcare system in Bangladesh, necessitating the elucidation of the genetic characteristics of this species. Five MDR, biofilm-forming <em>A. baumannii</em> strains isolated from clinical samples in Dhaka, Bangladesh, underwent a comprehensive whole-genome shotgun sequencing. <em>In silico</em> analyses determined strain types, antibiotic resistance, and virulence genes. The phylogenomic and pan-genome comparative analysis included the study samples along with an additional 15 Bangladeshi <em>A. baumannii </em>strains collected from the public database. The antibiotic-resistant profile showed that all the strains were resistant to meropenem. Two strains were resistant to polymyxin B up to 64 µg/ml. The average draft genome size of the strains was 3.93 Mbp, with about 39% GC content. Multilocus sequence typing characterized the strains as ST1, ST2, ST164, and ST623. All strains carried different classes of β-lactamase genes, including the carbapenem hydrolyzing enzyme coding genes <em>bla<sub>oxa</sub></em><em><sub>-23</sub></em><em> </em>and <em>bla<sub>oxa</sub></em><em><sub>-51</sub></em><em>.</em> One of the five strains studied harbored <em>bla</em><em><sub>NDM-9</sub></em> and <em>bla</em><em><sub>NDM-23</sub></em> genes. The phosphotransferase group of aminoglycosides modifying enzymes was the most common. All the strains had relatively similar virulence profiles. Pan-genome analysis revealed 1944 core and 1302 unique genes. Phylogenomic analysis revealed the distribution of these strains in different clusters with other MDR strains isolated worldwide. The study results suggest the implementation of antibiotic stewardship and genomic surveillance of MDR <em>A. baumannii</em> to control the spread of antibiotic resistance in Bangladesh.</p>",
"DOI": "10.5455/jabet.2025.06",
"views": 993,
"downloads": 129,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/17/178-1726215483-Figure1.jpeg",
"caption": "Figure 1. Antibiotic resistance phenotype in studied isolates. Here, blue, beige, and cyan boxes represent resistant, sensitive, and intermediately sensitive to a particular antibiotic. AMK: amikacin, GEN: gentamicin, IPM: imipenem, MEM: meropenem, CRO: ceftriaxone, FEP: cefepime, CIP: ciprofloxacin, LVX: levofloxacin, SXT: trimethoprim-sulfamethoxazole, AMP: ampicillin, TZP: piperacillin-tazobactam, CST: colistin, PMB: polymyxin B, and TGC: tigecycline.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/17/178-1726215483-Figure2.jpg",
"caption": "Figure 2. Distribution of predicted antibiotic resistance gene classes in the studied strains and the reference strain A. baumannii K09-14. Here, the blue box represents the presence, and the beige box represents the absence of a specific gene.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/17/178-1726215483-Figure3.jpg",
"caption": "Figure 3. Distribution of predicted virulence factor genes across the studied strains and the reference strain A. baumannii K09-14. Here, the red box represents the presence, and the beige box represents the absence of a specific gene.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/17/178-1726215483-Figure4.jpg",
"caption": "Figure 4. Phylogenomic tree of 50 A. baumannii strains collected from all over the world. Blue-colored strains are from Bangladesh. The labels in red boxes denote Bangladeshi strains collected in this study.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/17/178-1726215483-Figure5.jpg",
"caption": "Figure 5. Distribution of core and pan-genome in A. baumannii genomes collected from Bangladesh.",
"featured": false
}
],
"authors": [
{
"id": 1540,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh"
}
],
"first_name": "Sadia",
"family_name": "Ferdous",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-4720-4727",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1541,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh"
}
],
"first_name": "K M Shayerul Abedin",
"family_name": "Shayer",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1542,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh"
}
],
"first_name": "Afia",
"family_name": "Anjum",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0009-0005-3260-3556",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1543,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh"
}
],
"first_name": "Sabbir R.",
"family_name": "Shuvo",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0002-5539-5834",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1544,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh"
}
],
"first_name": "Ishrat",
"family_name": "Jabeen",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0001-6081-0337",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1545,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh"
}
],
"first_name": "Kristi Paul",
"family_name": "Hridi",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1546,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh"
}
],
"first_name": "A.K.M. Imrul",
"family_name": "Hassan",
"email": null,
"author_order": 7,
"ORCID": "http://orcid.org/0009-0001-5482-5994",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1548,
"affiliation": [
{
"affiliation": "Department of Genetics, Genomics and Bioinformatics, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, USA"
}
],
"first_name": "Md Imran",
"family_name": "Khan",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1549,
"affiliation": [
{
"affiliation": "Department of Soil, Water and Environment, University of Dhaka, Dhaka, Bangladesh"
},
{
"affiliation": "DNA Solution Ltd, Dhaka, Bangladesh"
}
],
"first_name": "Mohammad Fazle Alam",
"family_name": "Rabbi",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1550,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Mahmud",
"family_name": "Hossain",
"email": null,
"author_order": 10,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1551,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh"
}
],
"first_name": "Kazi Nadim",
"family_name": "Hasan",
"email": null,
"author_order": 11,
"ORCID": "http://orcid.org/0000-0003-4120-9233",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 314
},
{
"id": 1547,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh"
}
],
"first_name": "Sohidul",
"family_name": "Islam",
"email": null,
"author_order": 12,
"ORCID": "http://orcid.org/0000-0003-4003-2544",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sohidul Islam, Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh, Email: sohidul.islam@northsouth.edu",
"article": 314
}
]
},
{
"id": 313,
"slug": "178-1721019806-nanoparticles-of-beetroot-extract-a-potential-antimalarial-adjuvant-in-plasmodium-berghei-infected-mice",
"type": "short_communication",
"manuscript_id": "178-1721019806",
"published": "2024-12-09",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/15/178-1721019806.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 55-63",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Hardimarta FP, Suromo LB, et al. Nanoparticles of beetroot extract: A potential antimalarial adjuvant in Plasmodium berghei-infected mice. J Adv Biotechnol Exp Ther. 2025; 8(1): 55-63.",
"title": "Nanoparticles of beetroot extract: A potential antimalarial adjuvant in <span>Plasmodium berghei</span>-infected mice",
"abstract": "<p>Malaria disease is caused by a variant of mosquitoes that leads to transmission in humans. This study aimed to investigate the effects of beetroot extract (NBE) nanoparticles on parasitemia index and ICAM-1 levels in mice infected with <em>Plasmodium berghei</em>. Thirty male mice were randomly divided into six groups including H= baseline (normal/healthy), NC= <em>P. berghei</em> inoculation, PC= <em>P. berghei</em> inoculation treated with artemisinin, T1= <em>P. berghei</em> inoculation treated with artemisinin and NBE (50 mg/kg), T2= <em>P. berghei</em> inoculation treated with artemisinin and NBE (100 mg/kg), and T3= <em>P. berghei</em> inoculation treated with artemisinin and NBE (200 mg/kg). Parasitemia index in groups T2 (13.1%) and T3 (12.5 %) were significantly lower compared to groups NC (36 %) and PC (16.2 %). Furthermore, ICAM-1 levels in groups T2 (145.72 ng/ml) and T3 (151.24 ng/ml) were significantly lower compared to groups NC (319.17 ng/ml) and PC (241.93 ng/ml). The findings of the study suggest that nanoparticles of beetroot extract could have potential as an anti-inflammatory and antimalarial adjuvant for malaria infection.</p>",
"DOI": "10.5455/jabet.2025.05",
"views": 566,
"downloads": 97,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/30/09/178-1721019806-Figure1.jpg",
"caption": "Figure 1. Effect of nanoparticle of beetroot extract in the parasitemia index on mice inoculated with Plasmodium berghei ANKA and treated with artemisinin. n=5 at five days interval after treatment. Figures indicate the median value, B: Baseline, NC= negative control (inoculated with P. berghei), PC= positive control (inoculated with P. berghei + artemisinin 0.036 mg/g), T1= treatment 1(inoculated with P. berghei + artemisinin 0.036 mg/g+ nanoparticle of beetroot extract 50 mg/kg), T2= treatment 2 (inoculated with P. berghei + artemisinin 0.036 mg/g+ nanoparticle of beetroot extract 100 mg/kg), T3= treatment 3(inoculated with P. berghei + artemisinin 0.036 mg/g+ nanoparticle of beetroot extract 200 mg/kg). Data is presented as median (n=5 for each group), * p<0.05 compared to the baseline, and # p<0.05 compared to the negative control.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/30/09/178-1721019806-Figure2.jpg",
"caption": "Figure 2. Effect of Beetroot Extract Nanoparticle in the ICAM-1 serum level on mice inoculated with Plasmodium berghei ANKA and treated with artemisinin. n=5 at five days interval after treatment. Figures indicate the median value, B=Baseline (healthy control), NC= negative control (inoculated with P. berghei), PC= positive control (inoculated with P. berghei + artemisinin 0.036 mg/g), T1= treatment 1(inoculated with P. berghei + artemisinin 0.036 mg/g+ nanoparticle of beetroot extract 50 mg/kg), T2= treatment 2 (inoculated with P. berghei + artemisinin 0.036 mg/g+ nanoparticle of beetroot extract 100 mg/kg), T3= treatment 3 (inoculated with P. berghei + artemisinin 0.036 mg/g+ nanoparticle of beetroot extract 200 mg/kg). Values with superscripts are statistically significant when * p<0.05 compared to the baseline, and # p<0.05 compared to the negative control.",
"featured": false
}
],
"authors": [
{
"id": 1537,
"affiliation": [
{
"affiliation": "Doctoral Study Program of Medical and Health Science, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Fransisca Pramesshinta",
"family_name": "Hardimarta",
"email": "fransisca@unika.ac.id",
"author_order": 1,
"ORCID": "https://orcid.org/0009-0004-1484-9168",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Fransisca Pramesshinta Hardimarta, Doctoral Study Program of Medical and Health Science, Diponegoro University, Semarang, Central Java- 50192, Indonesia. Email: fransisca@unika.ac.id",
"article": 313
},
{
"id": 1538,
"affiliation": [
{
"affiliation": "Departement of Clinical Pathology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Lisyani Budipradigda",
"family_name": "Suromo",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0009-0004-2107-847X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 313
},
{
"id": 1539,
"affiliation": [
{
"affiliation": "Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Kis",
"family_name": "Djamiatun",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0001-7939-9365",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 313
}
]
},
{
"id": 312,
"slug": "178-1724568467-gonadal-health-benefits-of-black-cumin-nigella-sativa-seed-oil-on-chronic-lead-exposed-male-wistar-rats",
"type": "original_article",
"manuscript_id": "178-1724568467",
"published": "2024-12-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/33/178-1724568467.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 44-54",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Mustari A, Alam M, et al. Gonadal health benefits of black cumin (Nigella sativa) seed oil on chronic lead-exposed male Wistar rats. J Adv Biotechnol Exp Ther. 2025; 8(1): 44-54.",
"title": "Gonadal health benefits of black cumin (<span>Nigella sativa</span>) seed oil on chronic lead-exposed male Wistar rats",
"abstract": "<p>Lead (Pb) is a highly toxic heavy metal that can cause harm even at extremely low concentrations and damage various organs, including those of reproduction. Medical herbs like <em>Nigella sativa</em> include antioxidants that have been shown to enhance spermatogenesis and male fertility. This research aimed to investigate the gonadal health benefits of <em>Nigella sativa</em> seed oil (NSO) as an antidote in male rats exposed to chronic Pb toxicity. Forty rats were divided into four groups: a control group, a group exposed to Pb acetate (75 mg/kg body weight), a group given NSO (1ml/kg feed), and a group exposed to Pb acetate (75 mg/kg body weight) along with NSO (1ml/kg feed). Pb was administered through water, while NSO with fed for 16 weeks. Rats were euthanized, blood was obtained, and serum was prepared to perform hormonal study. Sperm concentration and motility along with testis weight and diameter were measured. The testes were collected for histology. The findings indicated a significant drop in serum concentrations of testosterone and thyroxin (T4) in Pb-exposed rats, whereas NSO demonstrated a remarkable ability to restore these hormone levels. Moreover, Pb exposure led to a notable reduction in sperm motility, sperm concentration, testis weight, and testis diameter, accompanied by seminiferous tubule degeneration. However, the administration of NSO effectively counteracted the adverse effects induced by chronic Pb exposure in rats. Together, these findings revealed the gonadoprotective effects of NSO against lead toxicity, suggesting its preventive as well as therapeutic potential in preserving the testicular function of rats exposed to chronic Pb toxicity.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.04",
"views": 1289,
"downloads": 83,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/08/178-1724568467-Figure1.jpg",
"caption": "Figure 1. Effect of Pb and NSO (NSO) on testosterone and thyroxin (T4) concentration. Rats were kept untreated or treated with Pb and Pb plus NSO and the testosterone and thyroxin (T4) concentrations were tested. Data indicate mean ± SEM (n=10). One-way ANOVA with Bonferroni multiple comparison test. * p≤0.05 and ** p≤0.01",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/08/178-1724568467-Figure2.jpg",
"caption": "Figure 2. Effect of Pb and Nigella sativa oil (NSO) on Sperm motility and concentration. Rats were kept untreated or treated with Pb and Pb plus NSO and the sperm motility and concentration were determined. Data indicate mean ± SEM (n=10). One-way ANOVA with Bonferroni multiple comparison test. * p≤0.05 and ** p≤0.01, *** p≤0.00.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/08/178-1724568467-Figure3.jpg",
"caption": "Figure 3. Effect of Pb and Nigella sativa oil (NSO) on testis weight (3a) (mean weight of both left and right testis) and diameter (3b). Rats were kept untreated or treated with Pb and Pb plus NSO and the testis weight and diameter were calculated. Data indicate mean ± SEM(n=10). One-way ANOVA with Bonferroni multiple comparison test. * p≤0.05 and ** p≤0.01",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/08/178-1724568467-Figure4.jpg",
"caption": "Figure 4. Effects of Pb and Nigella sativa oil (NSO) on testis in male rat. Photomicrograph of testis of control (A1-2), Pb (B1-2), NSO (C1-2), and Pb along with NSO (D1-2) treated rats at 100x and 400x magnification. Bar = 50 µm and Bar = 20 µm indicate magnification (a-d). It shows degeneration of the seminiferous tubule (DST) in rats treated with Pb (b).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/08/178-1724568467-Figure5.jpg",
"caption": "Figure 5. Effect of Pb and NSO on Johnsen score (functionality of seminiferous tubule) in Wistar male rats. Rats were kept untreated or treated with Pb and Pb plus NSO and the Johnsen score was quantified. Data indicate mean ± SEM (n=10). One-way ANOVA with Bonferroni multiple comparison test was performed. * p≤0.05 and *** p≤0.001.",
"featured": false
}
],
"authors": [
{
"id": 1531,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Afrina",
"family_name": "Mustari",
"email": "afrina.mustari@bau.edu.bd",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-7651-5487",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Afrina Mustari, Department of Physiology, Faculty of Veterinary Science, Bangladesh, Agricultural University, Mymensigh-2202, Bangladesh.\r\nEmail: afrina.mustari@bau.edu.bd",
"article": 312
},
{
"id": 1532,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Mahabub",
"family_name": "Alam",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-6871-4286",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 312
},
{
"id": 1533,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Samia",
"family_name": "Rashid",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0009-0002-4610-0675",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 312
},
{
"id": 1534,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "A.G.M.Sofi Uddin",
"family_name": "Mahamud",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-5779-6359",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 312
},
{
"id": 1535,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Mohammad Alam",
"family_name": "Miah",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0003-2778-9614",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 312
},
{
"id": 1536,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Emdadul Haque",
"family_name": "Chowdhury",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-8212-7923",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 312
}
]
},
{
"id": 311,
"slug": "178-1717636384-polymorphisms-in-the-leptin-gene-and-their-role-in-adolescent-obesity-and-metabolic-health",
"type": "original_article",
"manuscript_id": "178-1717636384",
"published": "2024-12-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/29/178-1717636384.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 32-43",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University Bangladesh",
"cite_info": "Tanzila RA, Irfannuddin, et al. Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health. J Adv Biotechnol Exp Ther. 2025; 8(1): 32-43.",
"title": "Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health",
"abstract": "<p>The leptin (LEP) gene polymorphism is one of the most common polymorphisms and is believed to be associated with high circulating LEP levels that can occur in obese individuals. Thus, this study purposed to determine the relationship of LEP1 and LEP2 gene polymorphisms with overweight/obese and non-obese adolescents in Palembang and their correlation with various metabolic and anthropometric parameters. This research was an analytical and observational study. A total of 50 blood samples from adolescents (aged 10-18 years) were analyzed in this study. Standard laboratory protocols for serum lipid measurements, deoxyribonucleic acid (DNA) extraction, allele analysis by polymerase chain reaction (PCR), and LEP and insulin analysis by enzyme-linked immunosorbent assay (ELISA) were employed. The study revealed the allelic frequencies for gene LEP1: non-obese (G = 0.45, A = 0.55) and obese (G = 0.87, A = 0.13), and gene LEP2: non-obese (G = 0.54, A = 0.45) and obese (G = 0.67, A = 0.33). The frequencies of alleles in the LEP1 gene showed a statistically significant association with nutritional status (p = 0.011), while there was no significant association between genotypes in the LEP gene and nutritional status. The level of LEP was 6.39 + 1.12 in non-obese and 11.57 + 1.68 in obese, with p 0.000; and the triglyceride level was 82.56 ± 43.22 in non-obese and 113.72 ± 54.03 in obese, with p 0.029. In conclusion, this study shows that LEP levels and the polymorphism in the LEP1 gene are associated with obese adolescents.</p>",
"DOI": "10.5455/jabet.2025.03",
"views": 545,
"downloads": 80,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/53/07/178-1717636384-Figure1.jpg",
"caption": "Figure 1. PCR products of LEP1 gene on 2% agarose gel; genotypes were G/G: 414 bp, G/A: 414, 362, 242 bp, and A/A: 362, 242 bp. Line M: Ladder, Line 20, 21: Non-digest PCR product, Line 39-50: A/A, Line 35-36: G/A, Line 37-38: G/G. Respondents no. 20, 21, 35 and 36 are classified as non-obese, while respondents no. 37-50 are classified as obese.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/07/178-1717636384-Figure2.jpg",
"caption": "Figure 2. PCR products of LEP2 gene on 2% agarose gel; genotypes were G/G: 229 bp, G/A: 229, 187, 61 bp, and A/A: 187, 61 bp. Line M: Ladder, Line 1, 7: Non-digest PCR product, Line 2-4: A/A, Line 5-6, 8-16: G/A, Line 17-20: G/G. Respondents no. 1, 7, 8, 9, 11, 15-18 are classified as non-obese, while respondents no. 2-6, 10, 12-14, 20 are classified",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/07/178-1717636384-Figure3.jpg",
"caption": "Figure 3. Allelic frequencies for gene LEP1 and LEP2; Hardy–Weinberg Equilibrium. p: dominant allele; q: recessive allele. The population of non-obese individuals has more alleles G than the obese population, which tends to have more alleles A for the variation in the LEP1 and LEP2 genes. (A) Allele G: p=0.54, with a small margin of error. q=0.67, also with a slightly larger error bar than p. This suggests that in the q setting, allele G is more prevalent; allele A: p=0.45, slightly lower compared to q=0.33. Both the p and q frequency error bars are small and similar. In general, Allele G is more prevalent than allele A in both situations. This can point to a pattern in the population under study, requiring more research into the environmental or biological variables affecting these frequencies. (B) Allele G: p=0.54, with a small margin of error. q=0.67, also with a slightly larger error bar than p. Allele G is more frequent in the q context. Allele A: p=0.45, slightly lower compared to q=0.33. The error bars for both p and q frequencies are modest and comparable. Overall, allele G has higher frequencies in both contexts compared to allele A. This could suggest a trend in the population being studied, warranting further investigation into the biological or environmental factors influencing these frequencies.",
"featured": false
}
],
"authors": [
{
"id": 1519,
"affiliation": [
{
"affiliation": "Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Sriwijaya, Palembang, South Sumatra, Indonesia"
},
{
"affiliation": "Department of Physiology, Faculty of Medicine, Universitas Muhammadiyah Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Raden Ayu",
"family_name": "Tanzila",
"email": "tanzila@um-palembang.ac.id",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-1526-5381",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Raden Ayu Tanzila, Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia. \r\nEmail: tanzila@um-palembang.ac.id",
"article": 311
},
{
"id": 1520,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Medicine, Universitas Sriwijaya, Palembang, South Sumatra Indonesia"
}
],
"first_name": "Irfannuddin",
"family_name": null,
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-2217-367X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1521,
"affiliation": [
{
"affiliation": "Department of Pediatric, Faculty of Medicine, Universitas Sriwijaya, Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Yudianita",
"family_name": "Kesuma",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0001-5074-9907",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1522,
"affiliation": [
{
"affiliation": "Department of Public Health, Faculty of Medicine, Universitas Sriwijaya, Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Iche Andriyani",
"family_name": "Liberty",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0001-7697-6593",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1523,
"affiliation": [
{
"affiliation": "Departement of Pharmacology, Faculty of Medicine, Universitas Muhammadiyah Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Miranti Dwi",
"family_name": "Hartanti",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0001-6886-2086",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1524,
"affiliation": [
{
"affiliation": "Department of Cardiology, Faculty of Medicine, Universitas Muhammadiyah Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Ni Made Elva",
"family_name": "Mayasari",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1525,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Medicine, Universitas Muhammadiyah Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Wieke",
"family_name": "Anggraini",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1526,
"affiliation": [
{
"affiliation": "Department of Pediatric, Faculty of Medicine, Universitas Muhammadiyah Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Liza",
"family_name": "Chairani",
"email": null,
"author_order": 8,
"ORCID": "https://orcid.org/0009-0001-7395-6789",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1527,
"affiliation": [
{
"affiliation": "Department of Parasitology, Faculty of Medicine, Universitas Muhammadiyah Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Thia",
"family_name": "Prameswarie",
"email": null,
"author_order": 9,
"ORCID": "https://orcid.org/0000-0002-7019-6817",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1528,
"affiliation": [
{
"affiliation": "Department of Parasitology, Faculty of Medicine, Universitas Muhammadiyah Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Indri",
"family_name": "Ramayanti",
"email": null,
"author_order": 10,
"ORCID": "https://orcid.org/0000-0001-7301-3361",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1529,
"affiliation": [
{
"affiliation": "Medical Study Program, Faculty of Medicine, Universitas Muhammadiyah Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Wulan",
"family_name": "Aristi",
"email": null,
"author_order": 11,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
},
{
"id": 1530,
"affiliation": [
{
"affiliation": "Medical Study Program, Faculty of Medicine, Universitas Muhammadiyah Palembang, South Sumatra, Indonesia"
}
],
"first_name": "Delvi",
"family_name": "Larasati",
"email": null,
"author_order": 12,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 311
}
]
},
{
"id": 310,
"slug": "178-1720484032-occurrence-of-multi-antibiotic-resistant-bacteria-isolated-from-food-handlers-hands-and-utensils-at-different-restaurants-in-dhaka-bangladesh",
"type": "original_article",
"manuscript_id": "178-1720484032",
"published": "2024-11-12",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/13/178-1720484032.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 18-31",
"academic_editor": "Md Nurul Haider Rasel, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Akter S, Akter R, et al. Occurrence of multi-antibiotic resistant bacteria isolated from food handlers’ hands and utensils at different restaurants in Dhaka, Bangladesh. J Adv Biotechnol Exp Ther. 2025; 8(1): 18-31.",
"title": "Occurrence of multi-antibiotic resistant bacteria isolated from food handlers’ hands and utensils at different restaurants in Dhaka, Bangladesh",
"abstract": "<p>Restaurant customers consume food contaminated by germs, increasing the risk of foodborne disease. In this investigation, food handlers’ hands and utensils from many restaurants near Sadarghat launch station in Dhaka, Bangladesh, were tested to determine bacterial contamination incidence. Standard procedures were followed in isolation and identification of bacterial isolates. Of the 60 analyzed swabs, 46.7% showed a positive culture for one or more potential bacterial contaminants. The occurrence of <em>Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa,</em> and <em>Vibrio cholerae</em> in the hands of food handlers was 50%, 33.4%, 33.4%, and 23.4%, respectively, and those in utensils was 33.4%, 16.7%, 16.7%, and 10%, respectively. The majority of tested isolates of <em>P. aeruginosa</em> (100%) showed the highest resistance phenomenon to ampicillin and tetracycline, <em>V. cholerae</em> (100%) to ampicillin, <em>E. coli</em> (80%) to ampicillin and streptomycin, and <em>S. aureus</em> (100%) to ciprofloxacin. All of the examined isolates of <em>P. aeruginosa</em> were found to be most sensitive to piperacillin-tazobactam, <em>E. coli</em> to imipenem and nitrofurantoin, <em>S. aureus</em> to gentamicin, amikacin, tetracycline and chloramphenicol, and <em>V. cholerae</em> to piperacillin-tazobactam, imipenem and amikacin. Remarkably, 100% of <em>V. cholerae</em> isolates were multidrug-resistant (MDR), with the highest multiple antibiotic resistance index (MARI) score of 0.428. Among the examined isolates, 75% of bacterial isolates showed MDR phenotype, while 90% of isolates recorded a MARI value greater than 0.2. Due to the increased risk of food contamination or cross-contamination from food handlers' hands and utensils, the findings of this study indicate that restaurant hygiene standards are inadequate and might have detrimental effects on the general public's health. Consequently, this highlighted the significance of further training to enhance food workers' understanding of proper hand-washing techniques.<strong> </strong></p>",
"DOI": "10.5455/jabet.2025.02",
"views": 736,
"downloads": 104,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/14/178-1720484032-Figure1.jpg",
"caption": "Figure 1. Bacteria colonies on selective media plates. A) Colonies of P. aeruginosa (Green) on Cetrimide agar plate B) S. aureus (Yellow) on Mannitol Salt agar plate, C) E. coli (Pink) on MacConkey agar plate, D) E. coli (Dark centered with a green metallic sheen) on Eosin methylene blue agar plate E) V. cholerae (Yellow) on thiosulfate citrate bile sucrose agar plate F) V. cholerae (turquoise blue) on CHROMagarTM plate.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/14/178-1720484032-Figure2.jpg",
"caption": "Figure 2. Simplex PCR of S. aureus isolated from the swabs of food handlers’ hands and utensils. Marker (M): 100 bp DNA ladder; + ve control: S. aureus ATCC 29213; Lane 1-12: nuc (279 bp) gene amplification visualize band; Lane 1-10: nuc positive isolates recovered from food handlers’ hands and Lane 11-12: nuc positive isolates recovered from utensils.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/14/178-1720484032-Figure3.jpg",
"caption": "Figure 3. Multiplex PCR of V. cholerae isolated from swabs of food handlers’ hands and utensils. Marker (M): 100 bp DNA ladder; + ve control: V. cholerae ATCC BAA-238; Lane 1-10: toxR (779 bp) gene amplification visualize band; Lane 7, 8: ctxA (564 bp) gene amplification visualize band; Lane 1-7: toxR positive isolates recovered from food handlers’ hands and Lane 8-10: toxR positive isolates recovered from utensils.",
"featured": false
}
],
"authors": [
{
"id": 1516,
"affiliation": [
{
"affiliation": "Department of Botany, Jagannath University, Dhaka, Bangladesh"
}
],
"first_name": "Sumi",
"family_name": "Akter",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0009-5909-6821",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 310
},
{
"id": 1517,
"affiliation": [
{
"affiliation": "Department of Marketing, University of Development Alternative, Dhaka, Bangladesh"
}
],
"first_name": "Ratna",
"family_name": "Akter",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 310
},
{
"id": 1518,
"affiliation": [
{
"affiliation": "Directorate of Secondary and Higher Education, Ministry of Education, Dhaka, Bangladesh"
}
],
"first_name": "Md. Abdus",
"family_name": "Salam",
"email": "sami.11081978@gmail.com",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-8623-3862",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Abdus Salam, Directorate of Secondary and Higher Education, Ministry of Education, Dhaka, Bangladesh. Email: sami.11081978@gmail.com",
"article": 310
}
]
},
{
"id": 309,
"slug": "178-1722318500-computational-analysis-of-spanallium-sativumspan-compounds-to-identify-thermolabile-hemolysin-inhibitors-against-spanvibrio-alginolyticusspan-in-shrimp",
"type": "original_article",
"manuscript_id": "178-1722318500",
"published": "2024-10-14",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/23/178-1722318500.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2025; 8(1): 01-17",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Bari SM, Reza NB, et al. Computational analysis of Allium sativum compounds to identify thermolabile hemolysin inhibitors against Vibrio alginolyticus in shrimp. J Adv Biotechnol Exp Ther. 2025; 8(1): 01-17.",
"title": "Computational analysis of <span>Allium sativum</span> compounds to identify thermolabile hemolysin inhibitors against <span>Vibrio alginolyticus</span> in shrimp",
"abstract": "<p><em>Vibrio alginolyticus </em>is one of the major disease-causing bacteria in shrimp aquaculture.<em> </em>The widespread use of antibiotics in shrimp aquaculture to treat bacterial diseases has raised concerns about antibiotic resistance. As a result, alternative treatments, such as plant extract phytochemicals are being explored to mitigate these risks. This study aims to identify promising biologically active compounds from garlic (<em>Allium sativum</em>) that can inhibit the virulent protein thermolabile hemolysin of <em>V. alginolyticus</em>, which causes shrimp vibriosis. Various computational approaches, including molecular docking, pharmacokinetic analysis, and molecular dynamics simulation, were conducted to predict the compounds that can inhibit the phospholipase and hemolysis activities of the thermolabile hemolysin protein. Out of thirty-five compounds from <em>A. sativum</em>, protopine (CID 4970), gibberellin A7 (CID 92782), and gibberellic acid (CID 6466) demonstrated the strongest binding affinities, with scores of -9.4, -8.0, and -7.4 kcal/mol, respectively. Pharmacokinetic and toxicity analyses showed favorable drug-like properties for gibberellin A7 and gibberellic acid with no violations. Molecular dynamics simulations demonstrated that gibberellin A7 and gibberellic acid exhibited the highest stability over 100 nanoseconds. The investigation shows that gibberellin A7 and gibberellic acid from <em>A. sativum</em> have the potential to inhibit the virulent activity of thermolabile hemolysin. However, the study needs further in-vitro and in-vivo analysis to test our predicted results.</p>",
"DOI": "10.5455/jabet.2025.01",
"views": 1074,
"downloads": 172,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/17/178-1722318500-Figure1.jpg",
"caption": "Figure 1. An illustrative summary of the methodologies used to investigate the potential TLH inhibitors against Vibrio alginolyticus in shrimp.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/17/178-1722318500-Figure2.jpg",
"caption": "Figure 2. Protein-ligand interaction of TLH protein and three potential compounds of A. sativum with control resveratrol. A, B, C and D represent protopine, gibberellin A7, gibberellic acid and resveratrol (control), respectively. In the protein-ligand complexes (Left), the forest green surface represents the entire protein, yellow represents the ligands, light blue signifies non-polar bonds, and red indicates hydrogen bonds. In the 3D structure (Middle), the color crimson represents the ligands, and the green represents the receptor-interacting residues. The 2D structure (Right), illustrates various interactions with distinct colors: hydrogen bonds (green), alkyl interactions (pink), salt bridges (orange), and van der Waals interactions (light green).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/17/178-1722318500-Figure3.jpg",
"caption": "Figure 3. Root-mean-square deviation (RMSD) plots of protein-ligand interactions. Resveratrol (control), protopine, gibberellin A7, and gibberellic acid values were visualized in gray, yellow, red, and blue colors, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/17/178-1722318500-Figure4.jpg",
"caption": "Figure 4. Root-mean-square fluctuation (RMSF) plots of protein-ligand interactions. Fluctuations of amino acid residues of resveratrol (control), protopine, gibberellin A7, and gibberellic acid values were visualized in gray, yellow, red, and blue colors, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/17/178-1722318500-Figure5.jpg",
"caption": "Figure 5. Radius of gyration (Rg) plots depict protein-ligand interactions. Resveratrol (control), protopine, gibberellin A7, and gibberellic acid values were visualized in gray, yellow, red, and blue colors respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/17/178-1722318500-Figure6.jpg",
"caption": "Figure 6. Solvent-accessible surface area (SASA) analysis of top three compounds with control (control) one. The X-axis represents a time frame 0-100 ns, while the Y-axis represents SASA (Å). Resveratrol (control), protopine, gibberellin A7, and gibberellic acid values were visualized in gray, yellow, red, and blue colors respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/17/178-1722318500-Figure7.jpg",
"caption": "Figure 7. Calculation of binding interaction free energies for the screened drug complex system using the MM-PBSA (Molecular Mechanics Poisson-Boltzmann Surface Area) method.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/17/178-1722318500-Figure8.jpg",
"caption": "Figure 8. The scatter plots labeled A (protopine), B (gibberelin A7), and C (gibberellic acid), comparing the effects of different groups on PCA (Principal Component Analysis) results compared to control compound resveratrol. Score plots depicted data clusters in two colors (control-red and blue-tested), where each dot represented one-time point.",
"featured": false
}
],
"authors": [
{
"id": 1507,
"affiliation": [
{
"affiliation": "Department of Aquatic Animal Health Management, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh"
}
],
"first_name": "Sayed Mashequl",
"family_name": "Bari",
"email": "bari.aahm@sau.edu.bd",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-0074-9436",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sayed Mashequl Bari; Department of Aquatic Animal Health Management\r\nSher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh\r\nEmail: bari.aahm@sau.edu.bd",
"article": 309
},
{
"id": 1508,
"affiliation": [
{
"affiliation": "Fish Disease Laboratory, Department of Aquatic Animal Health Management, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Nafees Bin",
"family_name": "Reza",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0009-0009-3212-9304",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 309
},
{
"id": 1509,
"affiliation": [
{
"affiliation": "Fish Disease Laboratory, Department of Aquatic Animal Health Management, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Meamaching",
"family_name": "Marma",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0009-0002-5890-7388",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 309
},
{
"id": 1510,
"affiliation": [
{
"affiliation": "Department of Biotechnology & Genetic Engineering, Noakhali Science and Technology University, Noakhali-3814, Bangladesh"
}
],
"first_name": "Sk. Foisal",
"family_name": "Ahmed",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0001-9299-1240",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 309
},
{
"id": 1511,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Md Arif",
"family_name": "Hussain",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0001-5238-5940",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 309
},
{
"id": 1512,
"affiliation": [
{
"affiliation": "Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md Naimuddin",
"family_name": "Jabed",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0009-0009-3213-7267",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 309
},
{
"id": 1513,
"affiliation": [
{
"affiliation": "Department of Medicine, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md Alamgir",
"family_name": "Hossain",
"email": null,
"author_order": 7,
"ORCID": "https://orcid.org/0009-0001-2829-5141",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 309
},
{
"id": 1514,
"affiliation": [
{
"affiliation": "Institute for Plant Nutrition and Soil Science, Kiel University, Germany"
}
],
"first_name": "Maria",
"family_name": "Manzoor",
"email": null,
"author_order": 8,
"ORCID": "https://orcid.org/0000-0002-8939-6528",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 309
},
{
"id": 1515,
"affiliation": [
{
"affiliation": "Department of Chemistry, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Md Saiful",
"family_name": "Alam",
"email": null,
"author_order": 9,
"ORCID": "https://orcid.org/0009-0001-1440-3068",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 309
}
]
}
]
}
],
"title": "2025",
"slug": "2025",
"order": 8
},
{
"id": 7,
"issues": [
{
"id": 23,
"title": "Vol7 Issue3",
"cover": "https://jabet.bsmiab.org/media/issue_cover/Jabet_Coverpage_GTMb7HT.jpg",
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2024",
"slug": "2024-vol7-issue3",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 308,
"slug": "178-1717174234-investigation-of-the-pharmacological-characteristics-of-spansphaeranthus-indicusspan-flowers-using-phytochemical-analysis",
"type": "original_article",
"manuscript_id": "178-1717174234",
"published": "2024-09-29",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/24/178-1717174234.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 642-657",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Khanam A, Roman TS, et al. Investigation of the pharmacological characteristics of Sphaeranthus indicus flowers using phytochemical analysis. J Adv Biotechnol Exp Ther. 2024; 7(3): 642-657.",
"title": "Investigation of the pharmacological characteristics of <span>Sphaeranthus indicus</span> flowers using phytochemical analysis",
"abstract": "<p>Although the conventional usage of <em>Sphaeranthus</em> <em>indicus</em> flowers in medicine exists, there is a lack of research on the precise pharmacological characteristics and comprehensive phytochemical composition of its flowers, resulting in a knowledge gap on their whole therapeutic capabilities. The aim of this work is to identify and describe the phytochemicals found in the flowers of <em>Sphaeranthus</em> <em>indicus</em> and assess specific pharmacological effects, such as antioxidant, antibacterial, anti-inflammatory, thrombolytic, antidepressant, and antidiarrheal characteristics. The investigation revealed that the MESIF (methanol extract of <em>S. indicus </em>flowers) showed substantial antioxidant performance (IC<sub>50</sub> = 26.33) accompanied by a moderate phenolic content (93.33 ± 1.76). At 250 and 500 µg/ml, the extract exhibited modest to moderate antibacterial sensitivity against many tested pathogens and significantly reduced inflammation by protein denaturation (41.63 ± 0.11 and 70.52 ± 0.20). Furthermore, MESIF exhibited remarkable thrombolytic activity with a statistically significant (***P<0.001) lysis of clots. The extract showed substantial reduction in immobility in both the Forced Swim Test (FST) and Tail Suspension Test (TST) at doses of 200 mg/kg and 400 mg/kg (***p<0.001). It also a showed significant potent antidiarrheal activity (**p<0.01 at 200 mg/kg and ***p<0.001 at 400 mg/kg), effectively reducing diarrheal episodes in the test models. These findings suggest that the MESIF holds potential for developing treatments with antioxidant, antibacterial, anti-inflammatory, thrombolytic, antidepressant, and antidiarrheal effects. Further research is recommended to isolate specific bioactive compounds and elucidate the mechanisms underlying these pharmacological actions.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d55",
"views": 1100,
"downloads": 154,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/04/178-1717174234-Figure1.jpg",
"caption": "Figure 1. IC50 value of BHT and MESIF observed with DPPH. BHT = Tert-butyl-1-hydroxytoluene; MESIF = Methanol extract of S. indicus flower and SCV = Scavenging.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/04/178-1717174234-Figure2.jpg",
"caption": "Figure 2. The clot lysis activity of MESIS and SK. Values are represented as mean ± SEM of six consecutive experiments. One-way analysis of variance (ANOVA) followed by Dunnett’s test was employed where p<0.05 were considered statistically significant compared to the control. SK = Streptokinase; *** = Highly significant and MESIF = Methanol extracts of S. indicus flowers.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/04/178-1717174234-Figure3.jpg",
"caption": "Figure 3. Effects of MESIF on the (A) force swimming test and (B) tail suspension test. Each column represents the mean ± SEM (n = 5). One-way analysis of variance (ANOVA) followed by Dunnett’s test was employed where p<0.05 were considered statistically significant compared to the control. *** = Highly significant; (MESIF) = Methanol extracts of S. indicus flower.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/04/178-1717174234-Figure4.jpg",
"caption": "Figure 4. Effect of MESIF and loperamide HCl on castor oil induced diarrhea in mice. Values are expressed as mean ± SEM (n = 5). One-way analysis of variance (ANOVA) followed by Dunnett’s test was employed where **p<0.05 were considered statistically significant compared to the control. ** = Moderate significant; *** = Highly significant; HCl = Hydrochloric acid and (MESIF) = Methanol extracts of S. indicus flower.",
"featured": false
}
],
"authors": [
{
"id": 1499,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chattogram-4318, Bangladesh"
}
],
"first_name": "Afsana",
"family_name": "Khanam",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 308
},
{
"id": 1500,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chattogram-4318, Bangladesh"
}
],
"first_name": "Tahamina Sultana",
"family_name": "Roman",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 308
},
{
"id": 1501,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chattogram-4318, Bangladesh"
}
],
"first_name": "Afsana",
"family_name": "Ferdous",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 308
},
{
"id": 1506,
"affiliation": [
{
"affiliation": "Department of Pharmacy, University of Science and Technology Chittagong (USTC), Zakir Hossain Road, Chattogram-4202, Bangladesh"
}
],
"first_name": "Mohammed Jubair",
"family_name": "Siddique",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 308
},
{
"id": 1502,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chattogram-4318, Bangladesh"
}
],
"first_name": "Nadia Nowrin",
"family_name": "Antu",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 308
},
{
"id": 1503,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chattogram-4318, Bangladesh"
}
],
"first_name": "Mir Ezharul",
"family_name": "Hossain",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 308
},
{
"id": 1504,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chattogram-4318, Bangladesh"
}
],
"first_name": "Mohammad",
"family_name": "Arman",
"email": "mohammadarman778@gmail.com",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Arman; Department of Pharmacy, International Islamic University Chittagong, Kumira, Chattogram-4318, Bangladesh. Email. mohammadarman778@gmail.com",
"article": 308
},
{
"id": 1505,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chattogram-4318, Bangladesh"
}
],
"first_name": "Mohammad Nazmul",
"family_name": "Islam",
"email": "nazmul@iiuc.ac.bd",
"author_order": 8,
"ORCID": "http://orcid.org/0000-0002-4021-106X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Nazmul Islam; Department of Pharmacy, International Islamic University Chittagong, Kumira, Chattogram-4318, Bangladesh. Email: \r\nEmail: nazmul@iiuc.ac.bd",
"article": 308
}
]
},
{
"id": 307,
"slug": "178-1704620428-effect-of-nigella-sativa-extract-on-the-elevation-of-serum-mda-levels-in-aluminum-induced-oxidative-stress-in-rats",
"type": "short_communication",
"manuscript_id": "178-1704620428",
"published": "2024-09-28",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/36/178-1704620428.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 633-641",
"academic_editor": "Md. Atikur Rahman, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Qurnianingsih E, Wungu CDK, et al. Effect of Nigella sativa extract on the elevation of serum MDA levels in aluminum-induced oxidative stress in rats. J Adv Biotechnol Exp Ther. 2024; 7(3): 633-641.",
"title": "Effect of <span>Nigella sativa </span> extract on the elevation of serum MDA levels in aluminum-induced oxidative stress in rats",
"abstract": "<p>Aluminum (Al) constitutes approximately 8% of the Earth's surface. Al is extensively used in various industrial applications, including medicines, food additives, cosmetics and farming practices. However, Al poses a risk to human health when it enters the body, potentially leading to various pathological conditions. <em>Nigella sativa,</em> known for its active compound thymoquinone (TQ), has demonstrated anti-inflammatory and antioxidant properties. TQ is recognized for its potential in the prevention and treatment of numerous diseases. This study aimed to explore the effect of <em>N. sativa </em>extract (NSE) in preventing the elevation of malondialdehyde (MDA) levels in the blood serum of rats subjected to aluminum-induced oxidative stress. Twenty-four male Wistar rats were divided into four groups. Groups K1 and K2 were fed a standard diet, while groups K3 and K4 received NSE at doses 200 mg/kg/day and 400 mg/kg/day, respectively, administrated through oral gavage. After two weeks of NSE pretreatment, groups K2, K3, and K4 were exposed to AlCl3 (34 mg/kg, oral gavage) for an additional three weeks. There were no significant differences (P > 0.05) in body weight or hemoglobin levels among the groups. Interestingly, a significant reduction in MDA level (P < 0.05) was observed among groups. Notably, MDA levels were lower in the NSE-treated and control groups compared to the AlCl3-exposed group. In conclusion, NSE may have a positive effect against oxidative stress induced by Al exposure.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d54",
"views": 854,
"downloads": 220,
"figures": [],
"authors": [
{
"id": 1491,
"affiliation": [
{
"affiliation": "Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Ema",
"family_name": "Qurnianingsih",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 307
},
{
"id": 1492,
"affiliation": [
{
"affiliation": "Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
},
{
"affiliation": "Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Citrawati Dyah Kencono",
"family_name": "Wungu",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 307
},
{
"id": 1493,
"affiliation": [
{
"affiliation": "Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Siti",
"family_name": "Khaerunnisa",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 307
},
{
"id": 1494,
"affiliation": [
{
"affiliation": "Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Lina",
"family_name": "Lukitasari",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 307
},
{
"id": 1495,
"affiliation": [
{
"affiliation": "Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Ira",
"family_name": "Humairah",
"email": "ira-h@fk.unair.ac.id",
"author_order": 5,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ira Humairah; Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia. Email: ira-h@fk.unair.ac.id",
"article": 307
},
{
"id": 1496,
"affiliation": [
{
"affiliation": "Department of Anatomy, Histology and Pharmacology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Rimbun",
"family_name": "Rimbun",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 307
},
{
"id": 1497,
"affiliation": [
{
"affiliation": "Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Gwenny Ichsan",
"family_name": "Prabowo",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 307
},
{
"id": 1498,
"affiliation": [
{
"affiliation": "Postgraduate School, Universitas Airlangga, Surabaya, Indonesia"
},
{
"affiliation": "Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India"
}
],
"first_name": "Arif Nur Muhammad",
"family_name": "Ansori",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 307
}
]
},
{
"id": 304,
"slug": "178-1717239460-antioxidant-and-hepatoprotective-effects-of-solanum-villosum-leaf-extracts-against-acetaminophen-induced-mouse-model-of-hepatotoxicity",
"type": "original_article",
"manuscript_id": "178-1717239460",
"published": "2024-09-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/35/178-1717239460.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 620-632",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Osano KO, Machocho AK, et al. NgugiAntioxidant and hepatoprotective effects of Solanum villosum leaf extracts against acetaminophen-induced mouse model of hepatotoxicity. J Adv Biotechnol Exp Ther. 2024; 7(3): 620-632",
"title": "Antioxidant and hepatoprotective effects of <span>Solanum villosum </span> leaf extracts against acetaminophen-induced mouse model of hepatotoxicity",
"abstract": "<p><em>Solanum villosum</em> is an indigenous vegetable and ethno-medicine source in Africa. Scientific validation of their medicinal potential is required. The current study aimed to assess the antioxidant and hepatoprotective effects of <em>S. villosum</em> leaf methanol extracts (SVE) against acetaminophen-induced mouse model. The SVE antioxidant activities were evaluated by ferric reducing antioxidant capacity and hydroxyl radical scavenging activities <em>in vitro</em>. Hepatoprotective activities were assessed by automated hematological analysis, chemical analysis and specific biochemical assays of liver homogenates. Mice were orally administered with SVE at 30, 100, 300 and 500 mg/kg body weight. Hepatotoxicity was induced by acetaminophen 300 mg/kg bw intraperitoneally while silymarin 25 mg/kg bw orally was a positive control. The SVE exhibited a clear concentration-dependent antioxidant activity. Subsequently, SVE treatment significantly (P≤0.05) lowered granulocyte count, while there was a significant increase in lymphocyte count in acetaminophen-treated mice. Further, SVE significantly lowered the activities of alanine transaminase, aspartate transaminase, and alkaline phosphatase, but significantly increased γ-glutamyl-transferase activity in treated mice compared to the controls. However, SVE led to insignificant change in total proteins. Additionally, SVE treatment led to a significant rise in superoxide dismutase and glutathione peroxidase activities but, an insignificant change in catalase activity. In conclusion, SVE confers liver protection by oxidative stress prevention, antioxidant enzyme activation, immuno-modulation and hepatic tissue preservation. The results also support indigenous use of <em>S. villosum</em> to protect patients against drug-induced liver damage and enhance immunity. Further, dietary supplements of <em>S. villosum</em> could be an adjunct therapy in acetaminophen-induced hepatotoxicity.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d53",
"views": 811,
"downloads": 195,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/30/178-1717239460-Figure1.jpg",
"caption": "Figure 1. Hydroxyl scavenging activity of S. villosum MeOH extracts (SVE). Absorbance is taken in triplicates and expressed as mean ± SEM (n=3). Means marked by stars (*) are significantly different statistically by ANOVA and Turkey’s post hoc test at 95%, P≤0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/30/178-1717239460-Figure2.jpg",
"caption": "Figure 2. Ferric reducing antioxidant capacity of S. villosum MeOH leaves extracts (SVE). Absorbance values were taken in triplicates and expressed as mean ±SEM (n=3). Means marked by different letters are significantly different statistically by ANOVA and Turkey’s post hoc test at 95%, P≤0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/30/178-1717239460-Figure3.jpg",
"caption": "Figure 3. Effects of S. villosum leaf MeOH extract (SVE) on leukocytic parameters in acetaminophen-induced female mice. Groups are Control, ACP (acetaminophen 300 mg/kg), ACP+Sily (25 mg/kg bw), ACP+SVE 30 mg/kg, ACP+SVE100 mg/kg, ACP+SVE300 mg/kg, and ACP+SVE500 mg/kg. Variations in (A) white blood cell count {WBC}, (B) % lymphocytes {% LYM} and (C) % granulocytes {GRAN} were evaluated. Values represent mean ± SEM for five mice (n=5) treated separately. Bars marked with different superscript letters are statistically significant using One-way ANOVA followed by Turkey’s post hoc test, at 95% level (P ≤ 0.05).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/30/178-1717239460-Figure4.jpg",
"caption": "Figure 4. Effects of S. villosum leaf MeOH extracts (SVE) on antioxidant enzymes. Groups are Control, ACP (acetaminophen 300 mg/kg), ACP+Sily (25 mg/kg bw), ACP+SVE 30 mg/kg, ACP+SVE100 mg/kg, ACP+SVE300 mg/kg, and ACP+SVE500 mg/kg. Changes in (A) superoxide dismutase activity {SOD} (B) glutathione peroxidase {GPx} and (C) catalase {CAT} activities were analysed. Values are reported as mean ± SEM for five mice (n=5). Bars marked with different superscript letters are statistically significant using One-way ANOVA and Tukey’s post hoc test at 95%(P≤0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/30/178-1717239460-Figure5.jpg",
"caption": "Figure 5. In vitro antioxidant and hepatoprotective properties of SVE in ACP-induced mice model of hepatotoxicity. ++ = positive activity, ⇈ = statistically significant increase in activity, ⇊= statistically significant decrease in activity.",
"featured": false
}
],
"authors": [
{
"id": 1478,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya"
},
{
"affiliation": "Department of Biochemistry, School of Medicine, Uzima University, Kisumu, Kenya"
}
],
"first_name": "Kenneth Onyango",
"family_name": "Osano",
"email": "osanokenneth032@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-7829-1886",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Kenneth Onyango Osano, PhD; Department of Biochemistry, School of Medicine, Uzima University, Kisumu, Kenya. Email: osanokenneth032@gmail.com",
"article": 304
},
{
"id": 1479,
"affiliation": [
{
"affiliation": "Department of Chemistry, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Alex Kingori",
"family_name": "Machocho",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-1504-480X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 304
},
{
"id": 1480,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Mathew Piero",
"family_name": "Ngugi",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-0226-9092",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 304
}
]
},
{
"id": 306,
"slug": "178-1720029737-effect-of-lipoic-acid-on-the-expression-of-nonalcoholic-fatty-liver-disease-associated-genes-in-the-liver-of-rats-fed-a-hypercaloric-choline-deficient-diet",
"type": "short_communication",
"manuscript_id": "178-1720029737",
"published": "2024-09-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/42/178-1720029737.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 612-619",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Effect of lipoic acid on the expression of nonalcoholic fatty liver disease-associated genes in the liver of rats fed a hypercaloric choline-deficient diet. J Adv Biotechnol Exp Ther. 2024; 7(3): 612-619.",
"title": "Effect of lipoic acid on the expression of nonalcoholic fatty liver disease-associated genes in the liver of rats fed a hypercaloric choline-deficient diet",
"abstract": "<p>Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver pathologies. Molecular mechanisms of NAFLD pathogenesis are complex and still require further clarification. Thus, this study aimed to<em> </em>investigate the impact of lipoic acid (LA), widely used as an antioxidant, on the development of different links of NAFLD, including <em>de novo</em> lipogenesis, antioxidant and xenobiotic-metabolizing enzymes by gene expression analysis. The experiment was carried out on three groups of male Wistar rats received control diet, hypercaloric choline-deficient diet (HCCD) or HCCD with LA (HCCD+LA). In the liver of rats the expression of acetyl-CoA carboxylase 1 (<em>Acaca</em>), fatty acid synthase<em> </em>(<em>Fasn</em>), stearoyl-CoA desaturase (<em>Scd</em>) and transcriptional regulators sterol-regulatory element-binding protein SREBP (<em>Srebf1</em>), carbohydrate response element-binding protein ChREBP (<em>Mlxipl</em>), peroxisome proliferator-activated receptor alpha PPARα (<em>Ppara</em>), heme oxygenase-1 HO-1 (<em>Hmox1</em>) and nuclear erythroid 2-related factor 2 Nrf2 (<em>Nrf2</em>), cytochrome P450 1A1 CYP1A1 (<em>CYP1A1</em>) and aryl hydrocarbon receptor AhR (<em>AhR</em>), cytochrome P450 2E1 CYP2E1 (<em>CYP2E1</em>) genes were evaluated. Supplementation of HCCD with LA led to an even greater than in HCCD group decrease in <em>Scd</em> gene expression by 88% (p<0.05), as well as a marked suppression of <em>Ppara</em> and <em>Mlxipl</em> by 37% (p<0.05) and 27% (p<0.05), to an 80% (p<0.05) elevation of <em>Hmox1</em> gene expression relative to the HCCD group, had no pronounced effect on <em>CYP1A1</em> and <em>AhR</em> gene expression but resulted in a 38% (p<0.05) suppression of <em>CYP2E1</em> expression compared with that slightly elevated by HCCD. In conclusion, the current study showed that LA reduces lipogenesis <em>de novo</em>, restores the expression of <em>Hmox1 </em>gene of the antioxidant enzyme HO-1, diminished by the HCCD diet, and decreases HCCD-induced level of the expression of <em>CYP2E1</em> gene of CYP2E1 enzyme, a potential source of free radicals.</p>",
"DOI": "10.5455/jabet.2024.d52",
"views": 663,
"downloads": 117,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1720029737-Figure1.jpg",
"caption": "Figure 1. Effects of supplementation of hypercaloric choline-deficient diet (HCCD) with lipoic acid (LA) on: Fasn (A), Acaca (B), Scd (C) gene expression. Gene expression in all groups is normalized relative to the gene expression of housekeeping genes Actb and Gapdh. The data are presented in relative units, for each gene the values in the control group are taken as 100%. Results are expressed as median, interquartile range, maximum and minimum values. Designations: p < 0.05 vs. * Control; # HCCD+LA vs. HCCD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1720029737-Figure2.jpg",
"caption": "Figure 2. Effects of supplementation of hypercaloric choline-deficient diet (HCCD) with lipoic acid (LA) on: Ppara (A), Mlxipl (B), Srebf1 (C) gene expression. Gene expression in all groups is normalized relative to the gene expression of housekeeping genes Actb and Gapdh. The data are presented in relative units, for each gene the values in the control group are taken as 100%. Results are expressed as median, interquartile range, maximum and minimum values. Designations: p < 0.05 vs. # HCCD+LA vs. HCCD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1720029737-Figure3.jpg",
"caption": "Figure 3. Effects of supplementation of hypercaloric choline-deficient diet (HCCD) with lipoic acid (LA) on: Hmox1 (A), Nrf2 (B) gene expression. Gene expression in all groups is normalized relative to the gene expression of housekeeping genes Actb and Gapdh. The data are presented in relative units, for each gene the values in the control group are taken as 100%. Results are expressed as median, interquartile range, maximum and minimum values. Designations: p < 0.05 vs. # HCCD+LA vs. HCCD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1720029737-Figure4.jpg",
"caption": "Figure 4. Effects of supplementation of hypercaloric choline-deficient diet (HCCD) with lipoic acid (LA) on: CYP1A1 (A), AhR (B), CYP2E1 (C) gene expression. Gene expression in all groups is normalized relative to the gene expression of housekeeping genes Actb and Gapdh. The data are presented in relative units, for each gene the values in the control group are taken as 100%. Results are expressed as median, interquartile range, maximum and minimum values. Designations: p < 0.05 vs. * Control; # HCCD+LA vs. HCCD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1720029737-Figure5.jpg",
"caption": "Figure 5. Summary of the effect of lipoic acid on the expression genes in the liver of rats fed a hypercaloric choline-deficient diet.",
"featured": true
}
],
"authors": [
{
"id": 1488,
"affiliation": [
{
"affiliation": "Federal Research Centre of Nutrition and Biotechnology, Moscow, 109240, Ustinsky pr. 2/14, Russian Federation, Russia"
}
],
"first_name": "Anastasia",
"family_name": "Balakina",
"email": "balakina.a.s@yandex.ru",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-8559-5538",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Anastasia Balakina, Federal Research Centre of Nutrition and Biotechnology, Moscow, 109240, Ustinsky pr. 2/14, Russian Federation, Russia. Email: balakina.a.s@yandex.ru",
"article": 306
},
{
"id": 1489,
"affiliation": [
{
"affiliation": "Federal Research Centre of Nutrition and Biotechnology, Moscow, 109240, Ustinsky pr. 2/14, Russian Federation, Russia"
}
],
"first_name": "Nikita",
"family_name": "Trusov",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-1919-9297",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 306
},
{
"id": 1490,
"affiliation": [
{
"affiliation": "Federal Research Centre of Nutrition and Biotechnology, Moscow, 109240, Ustinsky pr. 2/14, Russian Federation, Russia"
}
],
"first_name": "Victor",
"family_name": "Tutelyan",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-4164-8992",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 306
}
]
},
{
"id": 305,
"slug": "178-1718872954-effect-of-aqueous-and-ethanol-extracts-of-spancucurbita-maximaspan-seed-on-fasting-blood-glucose-in-alloxan-induced-rats",
"type": "original_article",
"manuscript_id": "178-1718872954",
"published": "2024-09-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/42/178-1718872954.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 597-611",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Njoki MP, Rebecca W, et al. Effect of aqueous and ethanol extracts of Cucurbita maxima seed on fasting blood glucose in alloxan-induced rats. J Adv Biotechnol Exp Ther. 2024; 7(3): 597-611.",
"title": "Effect of aqueous and ethanol extracts of <span>Cucurbita maxima</span> seed on fasting blood glucose in alloxan-induced rats",
"abstract": "<p>Diabetes is a chronic disorder marked by hyperglycemia due to insulin resistance and insufficient insulin action, or both. Synthetic antidiabetic drugs available on the market are associated with severe effects, necessitating the need for alternative antidiabetic drugs. This investigation aimed to determine the antidiabetic potential and phytochemical profiles of aqueous and ethanol seed extracts of <em>Cucurbita maxima</em>. The seeds of <em>C. maxima</em> were airdried, milled, and extracted using distilled water and ethanol. The antidiabetic effect of the extracts was performed using Wistar rats weighing 160-180 g. Thirty-five rats were assigned to 7 groups: non-diabetic (control), diabetic, glibenclamide-treated, and aqueous or ethanol extract-treated (200 and 400 mg/kg body weight). Alloxan monohydrate was used to induce experimental diabetes. The treatments were orally administered for 28 days. Blood glucose levels and histopathological analysis were performed. The phytochemical screening was conducted utilizing liquid chromatography-mass spectrometry (LC-MS). In diabetic rats, the effects of the two extracts revealed a substantial reduction in levels of fasting blood glucose in a dose-dependent manner, suggesting an antidiabetic effect. The histopathological analysis revealed the restoration of pancreatic β-cells by the extracts. The LC-MS analysis identified phytocompounds in the extracts belonging to classes of flavonoids, phenolic acids, and fatty acids that are associated with antidiabetic effects. Interestingly, the ethanol extract had a better antidiabetic effect compared to the aqueous extract. In conclusion, the aqueous and ethanol extracts of <em>C. maxima</em> seed possess phytocompounds that can be used to develop novel antidiabetic agents with less severe effects.</p>",
"DOI": "10.5455/jabet.2024.d51",
"views": 846,
"downloads": 152,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/29/178-1718872954-Figure1.jpg",
"caption": "Figure 1. Hourly comparison of the effects of aqueous and ethanol seed extracts of C. maxima on levels of fasting blood glucose on alloxan-induced diabetes in rats. Aqueous and ethanol extract was treated to diabetic rats. Using an independent t-test, bars with distinct lowercase letters within each time differ significantly (p<0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/29/178-1718872954-Figure2.jpg",
"caption": "Figure 2. Day wise comparison of the effects of aqueous and ethanol seed extracts of C. maxima on levels of fasting blood glucose on alloxan-induced diabetes in rats. Aqueous and ethanol extract was treated to diabetic rats. Using an independent t-test, bars with distinct lowercase letters within each time differ significantly (p<0.05).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/29/178-1718872954-Figure3.jpg",
"caption": "Figure 3. Effects of aqueous and ethanol seed extracts of C. maxima on histopathology of pancreatic tissue on alloxan-induced diabetes in rats. A) Non-diabetic rats (control); normal pancreatic β-cells of the islets of Langerhans; B) Diabetic rats; degenerated pancreatic β-cells; C) glibenclamide was treated to diabetic rats (regenerated pancreatic β-cells); D) Ethanol seed extract at 200 mg/kg bw-treated diabetic rats (partially regenerated pancreatic β-cells), E) Ethanol seed extract at 400 mg/kg bw-treated diabetic rats (regenerated pancreatic β-cells), F) aqueous seed extract at 200 mg/kg bw-treated diabetic rats (partly regenerated pancreatic β-cells), and G) aqueous seed extract at 400 mg/kg bw-treated diabetic rats (regenerated pancreatic β-cells); magnification = x400.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/29/178-1718872954-Figure4.jpg",
"caption": "Figure 4. LC-MS chromatogram of aqueous seed extract of C. maxima (upper chromatogram = positive mode (MS (+), lower chromatogram = negative mode (MS (-)).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/29/178-1718872954-Figure5.jpg",
"caption": "Figure 5. LC-MS chromatogram of ethanol seed extract of C. maxima (upper chromatogram = positive mode (MS (+), lower chromatogram = negative mode (MS (-)).",
"featured": false
}
],
"authors": [
{
"id": 1481,
"affiliation": [
{
"affiliation": "Department of Zoology, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya"
},
{
"affiliation": "Department of Medical Sciences, School of Applied and Health Sciences, Technical University of Mombasa, Mombasa, Kenya"
}
],
"first_name": "Muchirah Peninah",
"family_name": "Njoki",
"email": "muchirahpeninah@tum.ac.ke",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-5223-7796",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Muchirah Peninah Njoki, Department of Medical Sciences, School of Applied and Health Sciences, Technical University of Mombasa, Mombasa, Kenya. Email: muchirahpeninah@tum.ac.ke",
"article": 305
},
{
"id": 1482,
"affiliation": [
{
"affiliation": "Department of Zoology, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya"
}
],
"first_name": "Waihenya",
"family_name": "Rebecca",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-4629-3481",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 305
},
{
"id": 1483,
"affiliation": [
{
"affiliation": "Department of Zoology, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya"
}
],
"first_name": "Muya",
"family_name": "Shadrack",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-8894-3905",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 305
},
{
"id": 1484,
"affiliation": [
{
"affiliation": "Department of Food Science, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya"
}
],
"first_name": "Anselimo",
"family_name": "Makokha",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0003-2168-3050",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 305
},
{
"id": 1485,
"affiliation": [
{
"affiliation": "Department of Pure and Applied Sciences, School of Applied and Health Sciences, Technical University of Mombasa, Mombasa, Kenya"
}
],
"first_name": "Laila",
"family_name": "Abubakar",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0003-1117-9387",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 305
},
{
"id": 1486,
"affiliation": [
{
"affiliation": "Department of Zoology, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya"
}
],
"first_name": "Simeon",
"family_name": "Mogaka",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-7579-824X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 305
},
{
"id": 1487,
"affiliation": [
{
"affiliation": "Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi, Kenya"
}
],
"first_name": "Hastings",
"family_name": "Ozwara",
"email": null,
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-9679-9421",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 305
}
]
},
{
"id": 303,
"slug": "178-1716501352-effect-of-polycyclic-aromatic-hydrocarbons-on-liver-function-in-basra-oil-company-workers",
"type": "original_article",
"manuscript_id": "178-1716501352",
"published": "2024-09-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/48/178-1716501352.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 586-596",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Al-Hussain MA, Abass HA, et al. Effect of polycyclic aromatic hydrocarbons on liver function in Basra oil company workers. J Adv Biotechnol Exp Ther. 2024; 7(3): 586-596.",
"title": "Effect of polycyclic aromatic hydrocarbons on liver function in Basra oil company workers",
"abstract": "<p>Polycyclic aromatic hydrocarbons (PAH) are considered one of the most widespread categories of environmental pollutants. This study was conducted to discover the effect of PAH from the oil fields pollution in Basra on liver function. The relationship between PAH in air compared to blood serum and its effect on liver function parameters were studied. 120 blood samples were collected from male workers aged between 25-40 years from 3 different regions. Group 1 was from the Rumella field, while group 2 was from the West Qurna field, and group 3 was from an uncontaminated area far from the oil fields in the city center (control). Higher levels of PAH were found in group 1 and group 2 in the blood serum as compared to control in both air and serum. In control, significantly higher levels of PAH were found in air as compared to serum levels. As for PAH levels in the air, a higher level was detected in group 1 compared to group 2, while a lower level was detected in control. As for liver function tests, the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and bilirubin were the higher level in group 1 and 2 compared to control. In contrast, the levels of albumin and total protein were lower in the group 1 and 2 compared to the control. In conclusion, it is suggested that environmental pollutants such as PAH may cause liver diseases by reducing the function of liver in human.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d50",
"views": 669,
"downloads": 122,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/27/178-1716501352-Figure1.jpg",
"caption": "Figure 1. Comparison of PAH levels in blood serum and air samples among three different regions (City Center (Control), Rumella, and West Qurna groups). A statistically significant difference was also found when comparing the two groups (Rumella and West Qurna). Asterisk (*) means it reached statistical significance (P < 0.05).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/27/178-1716501352-Figure2.jpg",
"caption": "Figure 2. Correlation between PAH and ALT levels for the city center, Rumella, and West Qurna groups. There was a significant (P> 0.001) positive correlation between the Rumella, and West Qurna groups (r=0.59).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/27/178-1716501352-Figure3.jpg",
"caption": "Figure 3. Correlation between PAH and AST levels for the city center, Rumella, and West Qurna groups. there was a significant (P> 0.001) positive correlation between the Rumella, and West Qurna groups (r=0.52).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/27/178-1716501352-Figure4.jpg",
"caption": "Figure 4. Correlation between PAH and ALP levels for the city center, Rumella, and West Qurna groups. There was a significant (P> 0.001) positive correlation between the Rumella, and West Qurna groups (r = 0.58).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/27/178-1716501352-Figure5.jpg",
"caption": "Figure 5. Correlation between PAH and TP levels for the City center, Rumella, and West Qurna groups. There was a weak correlation between the Rumella, and West Qurna groups (r =0.4).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/27/178-1716501352-Figure6.jpg",
"caption": "Figure 6. Correlation between PAH and Alb levels for the city center, Rumella, and West Qurna groups. There was a weak correlation between the Rumella, and West Qurna groups (r=0.33).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/27/178-1716501352-Figure7.jpg",
"caption": "Figure 7. Correlation between PAH and Bil levels for the city center, Rumella, and West Qurna groups. There was a significant (P> 0.001) with positive correlation between the Rumella, and West Qurna groups (r=0.57).",
"featured": false
}
],
"authors": [
{
"id": 1475,
"affiliation": [
{
"affiliation": "Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Baghdad, Iraq"
}
],
"first_name": "Mohammed A.",
"family_name": "Al-Hussain",
"email": "mohammed.alhussain5@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0009-0008-0748-0875",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammed A. Al-Hussain, College of Medicine Al-nahrain University, Baghdad, Iraq. Email:mohammed.alhussain5@gmail.com",
"article": 303
},
{
"id": 1476,
"affiliation": [
{
"affiliation": "Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Baghdad, Iraq"
}
],
"first_name": "Hend Ahmed",
"family_name": "Abass",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0009-0005-5323-4964",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 303
},
{
"id": 1477,
"affiliation": [
{
"affiliation": "Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Baghdad, Iraq"
}
],
"first_name": "Estabraq AR.",
"family_name": "Al-Wasiti",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-1291-0656",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 303
}
]
},
{
"id": 302,
"slug": "178-1718172529-impact-of-scutellarin-on-cyclophosphamide-induced-testicular-damage-in-sprague-dawley-male-rats",
"type": "original_article",
"manuscript_id": "178-1718172529",
"published": "2024-09-23",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/48/178-1718172529.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 572-585",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Hussein SA, Gatea FK, et al. Impact of scutellarin on cyclophosphamide-induced testicular damage in Sprague Dawley male rats. J Adv Biotechnol Exp Ther. 2024; 7(3): 572-585.",
"title": "Impact of scutellarin on cyclophosphamide-induced testicular damage in Sprague Dawley male rats",
"abstract": "<p>Cyclophosphamide is one of the most invasive chemotherapeutic drugs, which is commonly used despite its adverse effects and toxicities. Scutellarin is an herbal flavonoid with multiple pharmacological activities and clinical applications. The goal of the current study was to investigate whether scutellarin protected the gonads of male rats exposed to a single dose of cyclophosphamide. In the experiment, 21 male Sprague-Dawley male rats were divided into three groups (n=7/group). Control group; received normal saline for 12 days by mouth; Induction group; received normal saline for 12 days by mouth and a single dose of cyclophosphamide (100 mg/kg) intraperitoneally on day 6; and Scutellarin group; received intragastric scutellarin (100mg/kg) for 12 days and a single dose of cyclophosphamide (100mg/kg) intraperitoneally on day 6. At the end of treatment, the samples were collected, the gonadosomatic index (GSI) was calculated, sperm parameters, serum testosterone, testicular malondialdehyde (MDA), and glutathione peroxidase (GPx) levels were measured, and histopathological alternation was evaluated. The results showed that GSI, sperm parameters, testosterone, and GPx levels were notably declined, while the level of MDA was significantly higher in the induction group compared to the control group. Interestingly, these measures markedly improved in scutellarin treated group. Additionally, histopathological analysis revealed a substantial change in the testicular tissue structure and a decrease in Jonson’s score in the induction group, although scutellarin co-treatment considerably attenuated and even reversed these changes. In conclusion, the outcomes demonstrated that scutellarin may act as a protective agent that alleviates rats’ testicular toxicity caused by cyclophosphamide therapy. Further, the proposed process may be attributed to its potent antioxidant properties.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d49",
"views": 769,
"downloads": 155,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/51/25/178-1718172529-figure1.jpg",
"caption": "Figure 1. A schematic illustration of the experimental design for the study of testicular damage-induced by Cyclophosphamide. NS = normal saline, CP = cyclophosphamide, IP = intraperitoneal, PO = by mouth, MDA = malondialdehyde, GPx = glutathione peroxidase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/51/25/178-1718172529-figure2.jpg",
"caption": "Figure 2. Effect on gonadosomatic index (GSI) among studied groups. Data were expressed as Mean ± SD. a: comparison with Control group; b: comparison with induction group; *P significant at level <0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/51/25/178-1718172529-figure3.jpg",
"caption": "Figure 3. Effect of scutellarin on the sperm’s viability. A = control group; B = induction group; and C = scutellarin group. Blue arrow denotes viable sperm; red arrows indicate dead sperm; sperms stained with Eosin & Nigrosine; A, B, & C =100X magnification.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/51/25/178-1718172529-figure4.jpg",
"caption": "Figure 4. Effect on serum testosterone level among studied groups. Data were expressed as Mean ± SD. a: comparison with Control group; b: comparison with Induction group; *P significant at level <0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/51/25/178-1718172529-figure5.jpg",
"caption": "Figure 5. Effect on testicular oxidative stress markers among studied groups. A) Malondialdehyde evaluation and B) Glutathione peroxidase evaluation. Data were expressed as Mean ± SD. a: comparison with Control group; b: comparison with Induction group.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/51/25/178-1718172529-figure6.jpg",
"caption": "Figure 6. The histological section of testicular tissue in rats stained with H&E. A, B=Control group, C, D=Induction group, and E, F= Scutellarin group. Blue arrows denote the tubular lumen, black arrows the presence of sperm, and yellow arrows the epithelial layers of the lumen. A, C, and E= 10X magnification, B, D and F= 40X magnification.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/51/25/178-1718172529-figure7.jpg",
"caption": "Figure 7. Effect on Jonson’s score among studied groups. Data were expressed as Mean ± SD. a: comparison with Control group; b: comparison with Induction group; and *P significant at level <0.05.",
"featured": false
}
],
"authors": [
{
"id": 1473,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutics, College of Medicine, Al-Nahrain University, Kathimiya, Baghdad, Iraq"
}
],
"first_name": "Shaimaa A.",
"family_name": "Hussein",
"email": "shaimaa18113@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Shaimaa A. Hussein, PhD; Department of Pharmacology and Therapeutics, College of Medicine, Al-Nahrain University, Kathimiya, Baghdad, Iraq.\r\nEmail: shaimaa18113@gmail.com",
"article": 302
},
{
"id": 1474,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutics, College of Medicine, Al-Nahrain University, Kathimiya, Baghdad, Iraq"
}
],
"first_name": "Fouad Kadhim",
"family_name": "Gatea",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 302
}
]
},
{
"id": 301,
"slug": "178-1716080109-effect-of-geographical-and-agroclimatic-location-on-phytocompounds-and-antioxidant-activity-of-moringa-oleifera-leaves",
"type": "original_article",
"manuscript_id": "178-1716080109",
"published": "2024-09-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/55/178-1716080109.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 556-571",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Sukmawaty E, Ahmad A, et al. Effect of geographical and agroclimatic location on phytocompounds and antioxidant activity of Moringa oleifera leaves. J Adv Biotechnol Exp Ther. 2024; 7(3): 556-571.",
"title": "Effect of geographical and agroclimatic location on phytocompounds and antioxidant activity of <span>Moringa oleifera</span> leaves",
"abstract": "<p><em>Moringa oleifera </em>L.<em> </em>is renowned for its rich phytochemical content and significant biological activity. The influence of geographic location and agroclimatic conditions on phytochemical content and bioactivity of plants remains unknown. Therefore, this study aimed to analyze the impact of geographical and agroclimatic location on the phytochemical content, antioxidant activity, and antibacterial potential of <em>M. oleifera</em> leaves. Phytochemical content was measured qualitatively and quantitatively, as well as bioactivity analysis was carried out by testing antioxidant and antibacterial activity. Interestingly, three dominant compounds were identified in both samples using the molecular docking tool. Phytochemical analysis revealed the presence of a broad spectrum of secondary metabolites in both samples, which were collected from two different locations. The identified metabolites were alkaloids, steroids, terpenoids, saponins, tannins, phenols, coumarins, flavonoids, quinones, and glycosides. The accumulation of phenols, flavonoid, and antioxidants in sample M1 was higher compared to sample M2. The results further suggest the confirmation of OH, C=O, CO, aliphatic, and aromatic carbons functional groups in samples. The most dominant compound 9,12,15-Octadecatrienoic acid (Z, Z, Z) found in sample M2. Molecular docking analysis suggests that the detected compounds have promising antioxidant and antibacterial activity, but their potential may vary due to divergence of geographical location and agroclimatic conditions. In addition, geographical location and agroclimatic conditions influenced the potential of other phytochemical and bioactivity in <em>M. oleifera</em> leaves. In conclusion, the overall findings can be effective for screening environmental factors-based regulation of potential compounds in <em>M. oleifera</em> and their pharmacological interest.</p>",
"DOI": "10.5455/jabet.2024.d48",
"views": 1058,
"downloads": 183,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/23/178-1716080109-figure1.jpg",
"caption": "Figure 1. Antioxidant capacity of methanol extract of M. oleifera leaves from different geographical and agroclimatic environments (M1: high altitude and low temperature, M2: low altitude and high temperature) compared with Vitamin C as control. The high DPPH activity level (%) represents a high antioxidant efficiency of the sample.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/23/178-1716080109-figure2.jpg",
"caption": "Figure 2. FTIR Spectra results of methanol extract of M. oleifera leaves from different geographical and agroclimatic environments in the 4000-400 cm-1 area and T indicates transmittance.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/23/178-1716080109-figure3.jpg",
"caption": "Figure 3. A) M1 Chromatogram results of methanol extract of M. oleifera leaves from location 1; B) Chromatogram of Levoglucosan; C) Benzeneacetonitrile,4-hydroxy; and D) 1,3,4,5-Tetrahydroxycyclohexanecarboxylic acid. E) M2 Chromatogram results of methanol extract of M. oleifera leaves from location 2; F) Chromatogram Octadecatrienoic acid; G) Quinic acid, and H) n-Hexadecanoic acid, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/23/178-1716080109-figure4.jpg",
"caption": "Figure 4. 2D ligand-Cytochrome c peroxidase. A) Ascorbic acid- Cytochrome c peroxidase, B) Levoglucosan-Cytochrome c peroxidase, C) 1,3,4,5-tetrahydroxycyclohexanecarboxylic-acid-Cytochrome c peroxidase, D) Benzeneacetonitrile,4-hydroxy-acid-Cytochrome c peroxidase, E) Octadecatrienoic acid-Cytochrome c peroxidase, F) Quinic acid- Cytochrome c peroxidase, G) n-Hexadecanoic acid- Cytochrome c peroxidase.",
"featured": true
}
],
"authors": [
{
"id": 1463,
"affiliation": [
{
"affiliation": "Department of Chemistry, Hasanuddin University, Makassar 90245, Indonesia"
},
{
"affiliation": "Department of Biology, UIN Alauddin Makassar, Makassar 92118, Indonesia"
}
],
"first_name": "Eka",
"family_name": "Sukmawaty",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-8847-9698",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 301
},
{
"id": 1464,
"affiliation": [
{
"affiliation": "Department of Chemistry, Hasanuddin University, Makassar 90245, Indonesia"
},
{
"affiliation": "Institute for Research and Community Services, Hasanuddin University, Makassar 90245, Indonesia"
}
],
"first_name": "Ahyar",
"family_name": "Ahmad",
"email": "ahyarahmad@unhas.ac.id",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-0913-4721",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ahyar Ahmad, PhD; Department of Chemistry, Hasanuddin University, Makassar 90245, Indonesia. Email: ahyarahmad@unhas.ac.id",
"article": 301
},
{
"id": 1465,
"affiliation": [
{
"affiliation": "Department of Chemistry, Hasanuddin University, Makassar 90245, Indonesia"
}
],
"first_name": "Abdul",
"family_name": "Karim",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-8852-9990",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 301
},
{
"id": 1466,
"affiliation": [
{
"affiliation": "Department of Biology, Hasanuddin University, Makassar 90245, Indonesia"
}
],
"first_name": "Zaraswati",
"family_name": "Dwyana",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0001-5002-237X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 301
},
{
"id": 1467,
"affiliation": [
{
"affiliation": "Department of Pharmacy, School of Pharmacy YAMASI, Makassar, 90222, Indonesia"
}
],
"first_name": "Harningsih",
"family_name": "Karim",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0002-1428-5559",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 301
},
{
"id": 1468,
"affiliation": [
{
"affiliation": "Research Collaboration Center for KARST Microbes BRIN-LPPM, Hasanuddin University, Makassar 90245, Indonesia"
},
{
"affiliation": "Faculty of Forestry, Hasanuddin University, Makassar 90245, Indonesia"
}
],
"first_name": "Siti Halimah",
"family_name": "Larekeng",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-9749-9500",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 301
},
{
"id": 1469,
"affiliation": [
{
"affiliation": "Department of Biology, UIN Alauddin Makassar, Makassar 92118, Indonesia"
}
],
"first_name": "Masriany",
"family_name": "Masriany",
"email": null,
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-8618-9662",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 301
},
{
"id": 1470,
"affiliation": [
{
"affiliation": "Department of Biology, UIN Alauddin Makassar, Makassar 92118, Indonesia"
}
],
"first_name": "Zulkarnain",
"family_name": "Zulkarnain",
"email": null,
"author_order": 8,
"ORCID": "https://orcid.org/0000-0002-6004-9020",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 301
},
{
"id": 1471,
"affiliation": [
{
"affiliation": "Department of Biology, UIN Alauddin Makassar, Makassar 92118, Indonesia"
}
],
"first_name": "Devi",
"family_name": "Armita",
"email": null,
"author_order": 9,
"ORCID": "https://orcid.org/0000-0003-1298-8178",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 301
},
{
"id": 1472,
"affiliation": [
{
"affiliation": "Department of Biology, UIN Alauddin Makassar, Makassar 92118, Indonesia"
}
],
"first_name": "Rahmat Fajrin",
"family_name": "Alir",
"email": null,
"author_order": 10,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 301
}
]
},
{
"id": 300,
"slug": "178-1716886536-effects-of-clove-and-tulsi-supplementation-on-the-dynamics-and-cellularity-of-adipose-tissue-in-the-visceral-and-subcutaneous-fat-depots-in-broiler",
"type": "original_article",
"manuscript_id": "178-1716886536",
"published": "2024-07-09",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/55/178-1716886536.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 546-555",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Islam R, Sinza SI, et al. Effects of Clove and Tulsi supplementation on the dynamics and cellularity of adipose tissue in the visceral and subcutaneous fat depots in Broiler.J Adv Biotechnol Exp Ther. 2024; 7(3): 546-555.",
"title": "Effects of Clove and Tulsi supplementation on the dynamics and cellularity of adipose tissue in the visceral and subcutaneous fat depots in Broiler",
"abstract": "<p>The substantial genetic enhancement of broiler chickens, coupled with the use of growth-promoting additives in feed, has led to accelerated growth rates, and elevated fat deposition in broiler. Clove and Tulsi, well-known medicinal plants, have been shown to improve both the growth performance and intestinal health of broilers. This study aimed to investigate the impact of Clove and Tulsi supplementation on the composition and cellular makeup in visceral and subcutaneous fat depots of broiler chickens. Sixty Cobb-500 broiler chicks were randomly divided into four groups to receive different supplemental treatments via drinking water. The groups included S0 (control), receiving no additional supplements; S1, receiving 0.5% Clove and 2% Tulsi; S2, receiving 1% Clove and 3% Tulsi; and S3, receiving 1.5% Clove and 4% Tulsi from day 8 to 28. On days 14 and 28, five broilers from each group were euthanized, and their visceral and subcutaneous fat depots were collected, weighed, and subjected to histological analysis to assess adipocyte histomorphology and histomorphometry. The S1 and S2 groups exhibited higher relative percentages of visceral fat depot, larger adipocyte size, and a greater percentage of larger adipocytes compared to the control, while adipocyte density was lower. Conversely, the S3 group showed minimal deviation from the control. Notably, no significant histomorphological differences were observed among the experimental groups. These findings suggest that Clove and Tulsi supplementation may modulate adipose tissue dynamics and cellularity depending on the concentration of supplementation.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d47",
"views": 1174,
"downloads": 202,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/17/178-1716886536-figure1.jpg",
"caption": "Figure 1. The relative weights (%) of the visceral (A) and subcutaneous (B) fat depots in broiler. Here, S0 – Control, S1 - 0.5% Clove and 2% Tulsi, S2 - 1% Clove and 3% Tulsi; and S3 - 1.5% Clove and 4% Tulsi. Data are presented as mean ± standard error of the mean (SEM). Data were analyzed using one-way analysis of variance (ANOVA) with the post hoc Duncan’s Multiple Range Test (DMRT). Values with different alphabetic superscripts are significantly different from each other.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/09/178-1716886536-figure2.jpg",
"caption": "Figure 2. Histoarchitectures of the visceral adipose tissue of different groups of broilers. S0 – Control, S1 - 0.5% Clove and 2% Tulsi, S2 - 1% Clove and 3% Tulsi; and S3 - 1.5% Clove and 4% Tulsi. Hematoxylin-eosin staining. A – Adipocytes. Magnification 400X; Scale bar = 100 μm.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/09/178-1716886536-figure3.jpg",
"caption": "Figure 3. Histoarchitectures of the subcutaneous adipose tissue of different groups of broilers. S0 – Control, S1 - 0.5% Clove and 2% Tulsi, S2 - 1% Clove and 3% Tulsi; and S3 - 1.5% Clove and 4% Tulsi. Hematoxylin-eosin staining. A – Adipocytes. Magnification 400X; Scale bar = 100 μm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/09/178-1716886536-figure4.jpg",
"caption": "Figure 4. The density of adipocytes (number of adipocytes/microscopic field) within the visceral (A) and subcutaneous (B) fat depots. S0 – Control, S1 - 0.5% Clove and 2% Tulsi, S2 - 1% Clove and 3% Tulsi; and S3 - 1.5% Clove and 4% Tulsi. The density of adipocytes numerically decreased on day 28 compared to that of day 14, indicating the increased size of the adipocytes. However, no significant difference was observed among the groups on day 14 or 28. Data are presented as mean ± SEM. Data were analyzed using one-way ANOVA with the post hoc DMRT.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/09/178-1716886536-figure5.jpg",
"caption": "Figure 5. The size of adipocytes (μm2) within the visceral (A) and subcutaneous (B) fat depots. S0 – Control, S1 - 0.5% Clove and 2% Tulsi, S2 - 1% Clove and 3% Tulsi; and S3 - 1.5% Clove and 4% Tulsi. The size of the adipocytes increased on day 28 compared to day 14. However, the groups had no mentionable difference in size on days 14 or 28. Data are presented as mean ± SEM. Data were analyzed using one-way ANOVA with the post hoc DMRT.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/09/178-1716886536-figure6.jpg",
"caption": "Figure 6. The circularity index of adipocytes (randomly selected 20 adipocytes) of the visceral (A) and subcutaneous (B) fat depots. S0 – Control, S1 - 0.5% Clove and 2% Tulsi, S2 - 1% Clove and 3% Tulsi; and S3 - 1.5% Clove and 4% Tulsi.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/09/178-1716886536-figure7.jpg",
"caption": "Figure 7. The percentage (%) of smaller and larger adipocytes in the visceral (A) and subcutaneous (B) fat depots. S0 – Control, S1 - 0.5% Clove and 2% Tulsi, S2 - 1% Clove and 3% Tulsi; and S3 - 1.5% Clove and 4% Tulsi.",
"featured": false
}
],
"authors": [
{
"id": 1460,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-6765-2693",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 300
},
{
"id": 1461,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sadia Islam",
"family_name": "Sinza",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 300
},
{
"id": 1462,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Nasrin",
"family_name": "Sultana",
"email": "nasrin.sultana@bau.edu.bd",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0001-5498-1017",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nasrin Sultana, PhD; Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh, Bangladesh. Email: nasrin.sultana@bau.edu.bd",
"article": 300
}
]
},
{
"id": 299,
"slug": "178-1714120555-tracing-mitochondrial-dysfunction-pathways-in-type-2-diabetes-the-promise-of-mitochondria-targeted-therapeutics",
"type": "review_article",
"manuscript_id": "178-1714120555",
"published": "2024-07-04",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/09/178-1714120555_eHIrdX2.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 530-545",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Amin T, Jerin N, et al. Tracing mitochondrial dysfunction pathways in type 2 diabetes: The promise of mitochondria-targeted therapeutics. J Adv Biotechnol Exp Ther. 2024; 7(3): 530-545.",
"title": "Tracing mitochondrial dysfunction pathways in type 2 diabetes: The promise of mitochondria-targeted therapeutics",
"abstract": "<p>The alarmingly high global prevalence of Type 2 Diabetes Mellitus (T2DM) has made novel treatment strategies imperative. One major contributing factor to the onset of T2DM is mitochondrial dysfunction, which deteriorates cellular health, interferes with energy metabolism, and reduces insulin sensitivity. Targeting mitochondrial pathways can enhance outcomes in T2DM. Promising therapeutic benefits can be derived from strategies that target and improve mitochondrial function, biogenesis, efficient turnover, and oxidative stress reduction. Through increased mitochondrial efficiency and improved insulin action, these treatments seek to address the complex problems associated with T2DM. The complexities of mitochondrial dynamics, such as their role in energy production, appropriate regulation of cell death, and creation of reactive oxygen species, highlight the necessity of comprehensive approaches in the development of medicines that target mitochondria. This strategy represents a step forward in the management of T2DM and highlights a promising area of cutting-edge research in the hunt for potent treatments that might stop or reverse the disease's development, improving patient outcomes and care.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d46",
"views": 1863,
"downloads": 275,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/35/04/178-1714120555-figure1.jpg",
"caption": "Figure 1. Potential mechanism of how skeletal muscle develops insulin resistance resulting from mitochondrial dysfunction. According to the model, intracellular fatty acyl CoA and diacylglycerol levels rise when mitochondrial fatty acid oxidation is inhibited due to dysfunctional mitochondria or decreased mitochondrial composition. These compounds trigger the activation of new protein kinase C, which in turn triggers a cascade of serine kinases that increases the serine phosphorylation (pS) of insulin receptor substrate-1 (IRS-1). The activity of phosphatidyl inositol 3-kinase (PI 3-kinase) is inhibited by increased serine phosphorylation of IRS-1, which prevents IRS-1 tyrosine phosphorylation by the insulin receptor. The suppression of insulin-stimulated glucose transport is a consequence of such inhibition. PIP: phosphatidylinositol 3, 4, 5-trisphosphate; PTB: phosphotyrosine binding domain; PH: pleckstrin homology domain; SH2: SRC homology domain; GLUT 4: Glucose transporter type 4; IRS 1: insulin receptor substrate-1.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/35/04/178-1714120555-figure2.jpg",
"caption": "Figure 2. Potential mechanism of mitochondria-targeted metabolic therapy through metformin-induced reduction of blood glucose level. Metformin inhibits mitochondrial respiratory chain complex I in the liver, causing a decrease in ATP synthesis and an increase in cellular levels of AMP. This decrease in hepatic gluconeogenic flux is ATP-dependent and inhibits enzymes involved in gluconeogenesis. Metformin also increases cellular redox potential and inhibits mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH)-dependent and complex IV inhibition-dependent mechanisms. Metformin directly inhibits mGPDH, resulting in an increased cytosolic redox state and reduced gluconeogenesis activity. It also raises the hepatic redox state through an increase in the glutathione to oxidized glutathione ratio (GSH -GSS), inhibiting genes encoding enzymes involved in gluconeogenesis. LDH: lactate dehydrogenase; OCT1: organic transporter 1; TET3: Tet methylcytosine dioxygenase; AMPK: AMP-activated protein kinase.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/35/04/178-1714120555-figure3.jpg",
"caption": "Figure 3. Mitochondria-targeted antioxidants therapy. Leukocyte-endothelium interactions in T2D patients can be regulated by antioxidants that target the mitochondria, such as Mitoquinone (MitoQ). When diabetic leukocytes were exposed to MitoQ, their generation of ROS from mitochondria was decreased. Treatment with MitoQ decreased both TNFα and NFκB-p65, which had increased in T2D. ROS: Reactive Oxygen species; TNF α: Tumor necrosis factor α; NF-κB: nuclear factor kappa Beta; MitoQ: mitochondria-targeted ubiquinone.",
"featured": false
}
],
"authors": [
{
"id": 1454,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka, Bangladesh"
}
],
"first_name": "Tasbir",
"family_name": "Amin",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-5265-8769",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 299
},
{
"id": 1455,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka, Bangladesh"
}
],
"first_name": "Nusrat",
"family_name": "Jerin",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0009-0002-3996-2226",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 299
},
{
"id": 1456,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka, Bangladesh"
}
],
"first_name": "Amana",
"family_name": "Hossain",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-1034-2173",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 299
},
{
"id": 1457,
"affiliation": [
{
"affiliation": "EskeGen Ltd., 6F, House 66, Road 8, Block C, Niketan, Gulshan 1, Dhaka 1212, Bangladesh"
}
],
"first_name": "Sadaf Saaz",
"family_name": "Siddiqi",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0009-0005-5640-0745",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 299
},
{
"id": 1458,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka, Bangladesh"
}
],
"first_name": "S M Bakhtiar UL",
"family_name": "Islam",
"email": "bakhtiar.islam@northsouth.edu",
"author_order": 5,
"ORCID": "https://orcid.org/0000-0002-3335-3289",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "S M Bakhtiar UL Islam, PhD; Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka, Bangladesh. \r\nEmail: bakhtiar.islam@northsouth.edu",
"article": 299
},
{
"id": 1459,
"affiliation": [
{
"affiliation": "Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University, Dhaka-1000, Bangladesh"
}
],
"first_name": "Shahjada",
"family_name": "Selim",
"email": "selimshahjada@bsmmu.edu.bd",
"author_order": 6,
"ORCID": "https://orcid.org/0000-0001-7749-3542",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Shahjada Selim, MD; Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University, Dhaka-1000, Bangladesh. \r\nEmail: selimshahjada@bsmmu.edu.bd",
"article": 299
}
]
},
{
"id": 298,
"slug": "178-1716628408-prevalence-of-multidrug-resistant-esbl-producing-spanescherichia-colispan-isolated-from-beef-and-sheep-meat-in-sylhet-bangladesh",
"type": "original_article",
"manuscript_id": "178-1716628408",
"published": "2024-07-03",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/09/178-1716628408.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 520-529",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Runa NS, Yesmin S, et al. Prevalence of multidrug-resistant ESBL-producing Escherichia coli isolated from beef and sheep meat in Sylhet, Bangladesh. J Adv Biotechnol Exp Ther. 2024; 7(3): 520-529.",
"title": "Prevalence of multidrug-resistant ESBL-producing <span>Escherichia coli</span> isolated from beef and sheep meat in Sylhet, Bangladesh",
"abstract": "<p>Nowadays, the spread of antibiotic-resistant <em>Escherichia coli (E. coli)</em> from food animals to humans is considered to be a serious public health problem. The aim of the study was to determine the prevalence of multidrug-resistant ESBL-producing <em>E. coli</em> isolated from beef and sheep meat. A total of 400 meat samples (200 beef and 200 sheep) were randomly collected from different slaughterhouses and wet markets in Habiganj, Sylhet, Moulvibazar and Sunamganj districts of the Sylhet division of Bangladesh. Among 400 samples, 136 <em>E. coli</em> were isolated from meat samples (90 beef and 46 sheep). Disc diffusion antimicrobial susceptibility assay was used to test the antimicrobial susceptibility traits of <em>E. coli</em>. The overall prevalence of multidrug resistance was 56.67% in <em>E. coli</em> of beef samples and 43.47% in <em>E. coli</em> of sheep meat. <em>E. coli</em> isolates of the meat samples (beef and sheep meat) were found to be 100% resistant to both Erythromycin and Ampicillin (100%), and 100% sensitive to Cotrimoxazole, Ciprofloxacin, Gentamycin, Levofloxacin, and Colistin. Furthermore, antibiotic sensitivity tests were performed using Cefotaxime, Ceftazidine, Ceftriaxone, and Aztreonam to know the prevalence of ESBL producers in isolated <em>E. coli.</em> ESBL-producing <em>E. coli</em>, which showed resistance to both Cefotaxime and Ceftriaxone, was found at 21.11 % (19/90) and 4.35% (2/46) in beef and sheep meat respectively. Our results showed that the best drugs to treat animals afflicted with ESBL-producing <em>E. coli</em> were Ceftazidine and Aztreonam, highlighting the urgent need to minimize the unnecessary use of antibiotics.</p>",
"DOI": "10.5455/jabet.2024.d45",
"views": 1378,
"downloads": 214,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/04/178-1716628408-figure1.jpg",
"caption": "Figure 1. Overall prevalence of beta-lactam antibiotic-resistant E. coli isolated from beef and sheep meat. Here, the total number of isolated E. coli is N=136 (90 from beef and 46 from sheep meat).Cefo = Cefotaxime, Cefta = Ceftazidime, Ceftri = Ceftriaxone, Az=Aztreonam, Cefo +Ceftri= Cefotaxime +Ceftriaxone.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/04/178-1716628408-figure2.jpg",
"caption": "Figure 2. Samples (136) were found as E. coli-positive from 400 meat samples (200 cattle meat and 200 sheep meat). All of the isolated samples were highly resistant to Ampicillin and Erythromycin but sensitive to Cotrimoxazole, Ciprofloxacin, and Gentamycin. In addition, 71 (out of 136) were found to MDR (Amp+Ery+Str). ESBL-producing E. coli, which showed resistance to both Cefotaxime and Ceftriaxone, was found 21.11%.",
"featured": true
}
],
"authors": [
{
"id": 1439,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary, Animal and Biomedical Sciences, Khulna Agricultural University, Khulna-9100, Bangladesh"
}
],
"first_name": "Nazmin Sultana",
"family_name": "Runa",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-1474-6965",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1440,
"affiliation": [
{
"affiliation": "Upazilla Livestock Office and Veterinary Hospital, Sreemangal Upazilla, Moulivibazar, Sylhet, Bangladesh"
}
],
"first_name": "Sabina",
"family_name": "Yesmin",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1441,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Asmaul",
"family_name": "Husna",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1442,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Nurjahan Yasmin",
"family_name": "Runa",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1443,
"affiliation": [
{
"affiliation": "Department of Poultry Science, Faculty of Veterinary, Animal and Biomedical Sciences, Khulna Agricultural University, Khulna-9100, Bangladesh"
}
],
"first_name": "Md. Sahidul",
"family_name": "Islam",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1444,
"affiliation": [
{
"affiliation": "Department of Animal science, Faculty of Veterinary, Animal and Biomedical Sciences, Khulna Agricultural University, Khulna-9100, Bangladesh"
}
],
"first_name": "Md. Asaduzzaman",
"family_name": "Lovelu",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1445,
"affiliation": [
{
"affiliation": "Department of Animal Nutrition, Faculty of Veterinary, Animal and Biomedical Sciences, Khulna Agricultural University, Khulna-9100, Bangladesh"
}
],
"first_name": "Mst Assrafi",
"family_name": "Siddika",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1446,
"affiliation": [
{
"affiliation": "Department of Medicine, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Tanvir",
"family_name": "Hasan",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1447,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Inzamamul",
"family_name": "Haque",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1448,
"affiliation": [
{
"affiliation": "Department of Microbiology and Parasitology, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh"
}
],
"first_name": "Mirza Synthia",
"family_name": "Sabrin",
"email": null,
"author_order": 10,
"ORCID": "https://orcid.org/0000-0001-5097-2535",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1449,
"affiliation": [
{
"affiliation": "Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Chamali Akter",
"family_name": "Shykat",
"email": null,
"author_order": 11,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1450,
"affiliation": [
{
"affiliation": "Department of Microbiology and Immunology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Moushumi",
"family_name": "Purkayastha",
"email": null,
"author_order": 12,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1451,
"affiliation": [
{
"affiliation": "Department of Microbiology and Public Health, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh"
}
],
"first_name": "Mohammad Ali",
"family_name": "Zinnah",
"email": null,
"author_order": 13,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1452,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Bashudeb",
"family_name": "Paul",
"email": null,
"author_order": 14,
"ORCID": "https://orcid.org/0000-0003-4407-168X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 298
},
{
"id": 1453,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Masudur",
"family_name": "Rahman",
"email": "rahmanmm.dpp@sau.ac.bd",
"author_order": 15,
"ORCID": "https://orcid.org/0000-0001-7095-9303",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Masudur Rahman, PhD;\r\nDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh. Email: rahmanmm.dpp@sau.ac.bd",
"article": 298
}
]
},
{
"id": 297,
"slug": "178-1707747121-dietary-supplementation-of-moringa-leaf-powder-improves-the-productive-and-reproductive-performance-of-pekin-duck",
"type": "original_article",
"manuscript_id": "178-1707747121",
"published": "2024-07-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/14/178-1707747121.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 507-519",
"academic_editor": "Md Jamal Uddin, PhD ; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Islam MM, Hoque SAM, et al. Dietary supplementation of moringa leaf powder improves the productive and reproductive performance of Pekin duck. J Adv Biotechnol Exp Ther. 2024; 7(3): 507-519.",
"title": "Dietary supplementation of moringa leaf powder improves the productive and reproductive performance of Pekin duck",
"abstract": "<p>Duck production is often considered advantageous compared to other poultry species due to its lower management requirements. However, intensive duck farming necessitates balanced nutrition and additional feed supplements to maximize profitability. Moringa leaves, rich in high-quality proteins, vitamins, minerals, and various bioactive compounds, have been proven to enhance the production and reproduction performance of poultry. The present study was conducted to elucidate the beneficial effect of Moringa leaf powder (MLP) on the productive and reproductive parameters of Pekin ducks. The experiment involved supplementing the basal ration of Pekin ducks with different percentages of MLP (0%, 2%, 4%, and 6%). The results indicated that 4% MLP supplementation improved production and reproduction parameters most effectively. This supplementation increased mature body weight and live weight gain by lowering the feed conversion ratio (FCR), enhancing the dressing percentage, and reducing triglycerides and cholesterol levels in blood lipids without adversely affecting other blood parameters. Notably, the ducks fed with 4% MLP-supplemented diet reached sexual maturity 7-10 days earlier than other MLP-treated or control groups. Additionally, MLP supplementation improved the fertility and hatchability of ducks. Remarkably, drakes fed with 4% MLP-supplemented diet showed increased semen volume and better sperm quality parameters, including total motility, progressive motility, viability, and concentration, with reduced sperm abnormalities compared to other MLP-treated and control groups. Therefore, it is suggested that 4% MLP can be supplemented in duck rations to enhance the productive and reproductive profiles.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d44",
"views": 1375,
"downloads": 169,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/02/178-1707747121-Figure1.jpg",
"caption": "Figure 1. The effects of MLP on the growth parameters of ducks. (A) represents the initial and final live weight, (B) represents the Live weight gain and Feed intake, and (C) represents the FCR. Values are Mean±SE; Values with different lowercase superscripts (a, b, or c) in the same parameter differ significantly (p<0.05). T1 (0% MLP) refers to control (only basal feed), T2 (2% MLP) refers to basal feed with 2% MLP, T3 (4% MLP) means basal feed with 4% MLP and T4 (6% MLP) means basal feed with 6% MLP.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/02/178-1707747121-Figure2.jpg",
"caption": "Figure 2. The follicular parameters of ducks treated with or without MLP. (A) represents the weight of the ovary, (B) represents the number of follicles, and (C) represents the length of follicles. SYF: Small Yellow Follicles and LWF: Large White Follicles. Values are Mean±SE; Values with different lowercase superscripts (a, b, or c) in the same parameter differ significantly (p<0.05). T1 (0% MLP) refers to control (only basal feed), T2 (2% MLP) refers to basal feed with 2% MLP, T3 (4% MLP) means basal feed with 4% MLP and T4 (6% MLP) means basal feed with 6% MLP.",
"featured": false
}
],
"authors": [
{
"id": 1434,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Md. Mazharul",
"family_name": "Islam",
"email": "mazhar@bsmrau.edu.bd",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "Md. Mazharul Islam, PhD; Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh. Email: mazhar@bsmrau.edu.bd",
"article": 297
},
{
"id": 1435,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
},
{
"affiliation": "Institute of Climate Change and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "S. A. Masudul",
"family_name": "Hoque",
"email": "mhoqueabg@bsmrau.edu.bd",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-3537-5892",
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "S. A. Masudul Hoque, PhD; Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh. Email: mhoqueabg@bsmrau.edu.bd",
"article": 297
},
{
"id": 1436,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Israt Jahan",
"family_name": "Meem",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 297
},
{
"id": 1437,
"affiliation": [
{
"affiliation": "Institute of Climate Change and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
},
{
"affiliation": "Department of Animal Science and Nutrition, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Abu Sadeque Md.",
"family_name": "Selim",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 297
},
{
"id": 1438,
"affiliation": [
{
"affiliation": "Institute of Food Safety and Processing, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
},
{
"affiliation": "Department of Dairy and Poultry Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Md. Morshedur",
"family_name": "Rahman",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 297
}
]
},
{
"id": 296,
"slug": "178-1698991618-noni-morinda-citrifolia-l-fruit-juice-reduces-paw-edema-and-protects-gastric-mucosal-injury-in-rats",
"type": "original_article",
"manuscript_id": "178-1698991618",
"published": "2024-07-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/14/178-1698991618.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 498-506",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Bangladesh",
"cite_info": "Ali AF, Mufaiduddin M, et al., Noni (Morinda citrifolia L.) fruit juice reduces paw edema and protects gastric mucosal injury in rats. J Adv Biotechnol Exp Ther. 2024; 7(3): 498-506.",
"title": "Noni (<span>Morinda citrifolia</span> L.) fruit juice reduces paw edema and protects gastric mucosal injury in rats",
"abstract": "<p>Edema is a reliable parameter to evaluate the anti-inflammatory process. Noni (<em>Morinda citrifolia</em> L.) fruit has been shown to decrease inflammation. However, studies on the impact of noni juice on gastric mucosal injury are still limited. Thus, the current study aimed to assess the effect of noni fruit juice as an anti-inflammatory agent in rat paw edema and gastric mucosal injury. The rats were divided into five groups such as control, carrageenan only, carrageenan and diclofenac sodium, carrageenan and 90% noni, and carrageenan + 100% noni. Paw edema was measured, and histopathological examination of the gastric tissues was performed. The administration of noni juice significantly reduced paw edema in the carrageenan + 90% noni and carrageenan + 100% noni groups of rats. Compared to the carrageenan + diclofenac sodium group, the carrageenan + 90% noni and carrageenan + 100% noni groups were not significantly different in reducing paw edema. It suggests that the administration of noni juice can reduce paw edema with the same potential as diclofenac sodium. Gastric mucosal injury in the carrageenan + diclofenac sodium group (scores 2 to 3) was higher than in the carrageenan + 90% noni and carrageenan + 100% noni groups (scores 1 to 2), both of them had significant differences (<em>p</em><0.05). In conclusion, noni juice can reduce paw edema and protect against gastric mucosal injury.</p>",
"DOI": "10.5455/jabet.2024.d43",
"views": 1235,
"downloads": 152,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/01/178-1698991618-Figure1.jpg",
"caption": "Figure 1. Paw edema volume decreased from 0 to 60 min. Control, carrageenan only (Carr), carrageenan and diclofenac sodium, carrageenan and 90% noni, and carrageenan + 100% noni. The mean is used to represent the values. *p=0.002; with repeated ANOVA test, #p=0.002; with Friedman test, and **p<0.0001; with repeated ANOVA test.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/01/178-1698991618-Figure2.jpg",
"caption": "Figure 2. Comparison of decreasing paw edema volume among groups. Control, carrageenan only (Carr), carrageenan and diclofenac sodium, carrageenan and 90% noni, and carrageenan + 100% noni. ΔV1, decreasing paw edema volume from 0-15 minutes; ΔV2, decreasing paw edema volume from 15-30 minutes; ΔV3, decreasing paw edema volume from 30-60 minutes; and ΔV4, decreasing paw edema volume from 0-60 minutes. The mean is used to represent the values. The min-max is used to represent the values. (*) p<0.05 compared with control group, (#) p<0.05 compared with carrageenan + diclofenac sodium group.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/01/178-1698991618-Figure3.jpg",
"caption": "Figure 3. Histopathology examination of gastric mucosa epithelial in A) control group; B) carrageenan + diclofenac sodium group; C) carrageenan + 90% noni group; D) carrageenan + 100% noni group. Gastric epithelial ulcer (black arrow); epithelial desquamation (red arrow).",
"featured": true
}
],
"authors": [
{
"id": 1430,
"affiliation": [
{
"affiliation": "Department of Medicine, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Alvira Firdausi",
"family_name": "Ali",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 296
},
{
"id": 1431,
"affiliation": [
{
"affiliation": "Department of Medicine, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Muhammad",
"family_name": "Mufaiduddin",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 296
},
{
"id": 1432,
"affiliation": [
{
"affiliation": "Department of Medicine, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Lingga",
"family_name": "Agustina",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 296
},
{
"id": 1433,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Desy",
"family_name": "Armalina",
"email": "desyarmalina@lecturer.undip.ac.id",
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-4708-7006",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Desy Armalina, Department of Anatomy and Histology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia. Email:desyarmalina@lecturer.undip.ac.id",
"article": 296
}
]
},
{
"id": 295,
"slug": "178-1716056133-metabolomic-signatures-of-ramadan-diurnal-intermittent-fasting-in-type-2-diabetes-mellitus",
"type": "original_article",
"manuscript_id": "178-1716056133",
"published": "2024-06-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/14/178-1716056133.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 482-497",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Akter T, Nath SD, et al. Metabolomic signatures of Ramadan diurnal intermittent fasting in type 2 diabetes mellitus. J Adv Biotechnol Exp Ther. 2024; 7(3): 482-497.",
"title": "Metabolomic signatures of Ramadan diurnal intermittent fasting in type 2 diabetes mellitus",
"abstract": "<p>The global prevalence of type 2 diabetes mellitus (T2DM) is on the rise and projections indicate that an alarming portion of the population will be affected by this disease in the coming decades. In T2DM, chronic hyperglycemia affects the metabolism of the human body. Previous studies on how diabetes impacts metabolism have yielded contradictory results. Therefore, to gain a deeper comprehension of the pathophysiology and progression of diabetes, it is crucial to pinpoint the dysregulated metabolism caused by the disease. In this study, we investigated the alterations in global metabolism in diabetic patients, as well as the effect of Ramadan diurnal intermittent fasting (RDIF) on metabolism. The levels of metabolites that are significantly different in diabetic individuals are components of five pathways: purine metabolism, cysteine and methionine metabolism, arginine and proline metabolism, porphyrin metabolism, and riboflavin metabolism. In diabetic individuals, RDIF significantly altered metabolite levels, affecting three pathways in particular: the pentose phosphate pathway, the tyrosine metabolism, and the D-arginine and D-ornithine metabolism. These pathways share genes previously linked to glycemic control and diabetes. Altogether, this study provides more insight into the beneficial effects of diurnal fasting in controlling diabetes. <strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d42",
"views": 1067,
"downloads": 193,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/30/178-1716056133-Figure1.jpg",
"caption": "Figure 1. Volcano plot of merged features of all diabetic and non-diabetic samples where the direction of comparison was diabetes/non-diabetes. 30 features (m/z ratio) are significantly elevated (red color-coded) and 102 features are significantly reduced (blue color-coded) in diabetic samples. FC: fold change.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/30/178-1716056133-Figure2.jpg",
"caption": "Figure 2. Mummichog pathway activity profile for the enriched pathway. The X-axis represents the enrichment factor, while the Y-axis signifies the negative logarithm of p-values. Hits (all) contain the number of empirical compound hits from the uploaded m/z ratio list. The enrichment factor of the pathways here is the ratio between the number of significant pathways hits from the uploaded m/z ratio list and the expected number of hits within that pathway.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/30/178-1716056133-Figure3.jpg",
"caption": "Figure 3. Integration of gene networks. The marked region encompasses the genes that are shared among arginine and proline metabolism pathways and the cysteine and methionine metabolism pathways.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/30/178-1716056133-Figure4.jpg",
"caption": "Figure 4. Volcano plot of all merged features of non-diabetic samples both before and after intermittent fasting. 41 features (m/z ratio) are significantly elevated (red color-coded) and 44 features are significantly reduced (blue color-coded) in the following RDIF non-diabetic samples.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/30/178-1716056133-Figure5.jpg",
"caption": "Figure 5. Volcano plot of all merged features of diabetic samples both before and after intermittent fasting. RDIF significantly elevated 98 features (red color-coded) and reduced 17 features (blue color-coded) in diabetic samples.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/30/178-1716056133-Figure6.jpg",
"caption": "Figure 6. Mummichog pathway activity profile for significantly upregulated and downregulated metabolites in diabetic samples compared to non-diabetic samples.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/30/178-1716056133-Figure7.jpg",
"caption": "Figure 7. Integration of gene networks. The marked region encompasses the genes that are shared among the pentose phosphate pathway and the tyrosine metabolism pathway.",
"featured": false
}
],
"authors": [
{
"id": 1423,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Tahmina",
"family_name": "Akter",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 295
},
{
"id": 1424,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Sudipta Deb",
"family_name": "Nath",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 295
},
{
"id": 1425,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Anik",
"family_name": "Biswas",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 295
},
{
"id": 1426,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Mahmudul Hasan",
"family_name": "Akash",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 295
},
{
"id": 1427,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Samayeta Sarkar",
"family_name": "Tuli",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 295
},
{
"id": 1428,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Sharif",
"family_name": "Akhteruzzaman",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 295
},
{
"id": 1429,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Abu Ashfaqur",
"family_name": "Sajib",
"email": "abu.sajib@du.ac.bd",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0003-1710-9865",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Abu Ashfaqur Sajib, PhD; Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh. Email: abu.sajib@du.ac.bd",
"article": 295
}
]
},
{
"id": 294,
"slug": "178-1716879233-an-assessment-on-the-impact-of-weaning-stress-on-the-weaning-age-in-black-bengal-goat-kids",
"type": "original_article",
"manuscript_id": "178-1716879233",
"published": "2024-06-29",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/21/178-1716879233.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 472-481",
"academic_editor": "Md Saidur Rahman, PhD; University of Missouri, USA",
"cite_info": "Ahmed S, Hemayet MA, et al. An assessment on the impact of weaning stress on the weaning age in Black Bengal goat kids. J Adv Biotechnol Exp Ther. 2024; 7(3): 472-481.",
"title": "An assessment on the impact of weaning stress on the weaning age in Black Bengal goat kids",
"abstract": "<p>Weaning is stressful for goat kids, often leading to stunted growth, weight loss, and mortality, with varied weaning times reducing overall productivity and profitability. The aim of this research was to determine the optimal age for weaning of Black Bengal goat (BBG) kids and evaluate the impact of weaning stress on their post-weaning performances. A trial was conducted comprising 4 groups (8 kids/group), accompanied by 4 lactating does, weaned at intervals of 45, 60, 75, and 90 days (designated as A, B, C, and D, respectively), and reared up to six months of age. Heart rate measurements and blood samplings were done three days before (-3), during (0), and three days after (+3) individual weaning schedules. Furthermore, dry matter intake, daily weight gain, weaning weight, 06 months weight, disease incidence, and kid mortality were also recorded. The serum concentration of triiodothyronine (T3) hormone declined significantly (p<0.05) at +3 days after weaning than -3 days before weaning in groups A, B, and C while an increase was observed in group D. The average cortisol level was significantly (p< 0.05) lower in group D than in other groups. Post-weaning performances, including weaning weight, 06 months weight, daily weight gain, and kid mortality also differ significantly (p<0.05). Group D demonstrated superior performance compared to groups A, B, and C. The findings revealed that kids weaned at 75 to 90 days experienced less stress, performed better, and had fewer health issues. <strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d41",
"views": 1134,
"downloads": 172,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/50/29/178-1716879233-Figure1.jpg",
"caption": "Figure 1. Pictures of taking blood and BPM from goats. A) Shows blood samples were collected from the jugular vein by using a vacuum blood collection tube and B) shows the number of heartbeats/minutes of the kids measured by using Polar-S810i heart rate monitors.",
"featured": true
}
],
"authors": [
{
"id": 1418,
"affiliation": [
{
"affiliation": "Goat Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh"
}
],
"first_name": "Sadek",
"family_name": "Ahmed",
"email": "sadek.ahmed@blri.gov.bd",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-9680-7372",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sadek Ahmed, PhD; Goat Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh. Email: sadek.ahmed@blri.gov.bd",
"article": 294
},
{
"id": 1419,
"affiliation": [
{
"affiliation": "Sheep Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh"
}
],
"first_name": "Md Abu",
"family_name": "Hemayet",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0009-0005-3661-8894",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 294
},
{
"id": 1420,
"affiliation": [
{
"affiliation": "Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh"
}
],
"first_name": "Md Habibur",
"family_name": "Rahman",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-3221-2142",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 294
},
{
"id": 1421,
"affiliation": [
{
"affiliation": "Sheep Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh"
}
],
"first_name": "Md Mahmudul Hasan",
"family_name": "Pasha",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-2144-3906",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 294
},
{
"id": 1422,
"affiliation": [
{
"affiliation": "Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural University, Sher-e-Bangla nagar, Dhaka, Bangladesh"
}
],
"first_name": "Nusrat",
"family_name": "Jahan",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0001-6163-4320",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 294
}
]
},
{
"id": 293,
"slug": "178-1716310832-impact-of-betel-quid-on-hyperglycemia-among-diabetes-patients-in-bangladesh",
"type": "original_article",
"manuscript_id": "178-1716310832",
"published": "2024-06-28",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/21/178-1716310832.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(3): 460-471",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka Bangladesh",
"cite_info": "Marzan AA, Hasan MS, et al. Impact of betel quid on hyperglycemia among diabetes patients in Bangladesh. J Adv Biotechnol Exp Ther. 2024; 7(3): 460-471.",
"title": "Impact of betel quid on hyperglycemia among diabetes patients in Bangladesh",
"abstract": "<p>Betel quid (BQ), a prevalent social habit in Asia, is often used without awareness of its harmful effects. This study explored the impact of BQ chewing on hyperglycemia among diabetic patients in northeastern Bangladesh. Specifically, this study focused on the role of areca nut, a key addictive component in BQ, in influencing hyperglycemia. Random blood glucose (RBG) tests were conducted on 961 diabetic patients, alongside lifestyle data, from August 2018 to February 2019 at Sylhet Diabetic Hospital, Bangladesh. Statistical analyses, including t-tests, Analysis of the variance (ANOVA), Fisher's exact test, and Multivariate regression, were employed to assess RBG status in BQ chewer vs. non-chewer. BQ chewers had higher RBG levels than non-chewers (263.3 ± 4.768 vs. 251.0 ± 5.915). Notably, raw areca nut users in the BQ group had significantly higher RBG levels than dry nut users (278.0 ± 8.790 vs. 252.1 ± 6.835 mg/dl), with up to 50 times more hyperglycemic effect. BQ chewing was associated with elevated RBG levels across different demographic and lifestyle groups. Lack of awareness was also prevalent among BQ chewers while individuals with desk jobs showed higher RBG levels. In conclusion, diabetic BQ chewers experience elevated hyperglycemia levels, highlighting the. urgent need to discourage BQ use for effective diabetes management.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d40",
"views": 1038,
"downloads": 109,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/30/178-1716310832-Figure1.jpg",
"caption": "Figure 1. Effect of BQ chewing and areca nut on diabetic patients. A) Patients with BQ chewing habit showed higher RBG levels (263.3 ± 4.768 mg/dl) than non-chewers (251.0 ± 5.915 mg/dl), which was non-significant (p value> 0.05). B) Higher RBG (278.0 ± 8.790 mg/dl) was found in raw nut chewer than in dry nut chewer patients (252.1 ± 6.835 mg/dl), which is statistically significant (p < 0.0186) in the student-t test. Glucose (Glc) levels are in mg/dl, represented as mean + SEM.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/30/178-1716310832-Figure2.jpg",
"caption": "Figure 2. The interplay of BQ chewing, nut consumption, and smoking habits with RBG level study participants. A) Influence of BQ chewing on smokers and nonsmokers participants. No significant difference in mean RBG level was found in smokers with or without BQ habit while a significant difference was found between nonsmokers BQ chewers and non-chewers. B) Among patients with a smoking habit, a significantly higher RBG mean was found in raw nut BQ chewer (305.0 ± 17.19 mg/dl) compared to no BQ chewer group (245.8 ± 9.718 mg/dl). C) Among patients with non-smoking habits, significantly higher RBG was found in the raw nut BQ chewer (291.5 ± 19.89 mg/dl) concerning the non-BQ chewer group (232.4 ± 15.48 mg/dl), using the student-t test. Glucose levels are in mg/dl, represented as mean + SEM.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/30/178-1716310832-Figure3.jpg",
"caption": "Figure 3. Association between lifestyle parameters in RBG of diabetic patients. A) BQ chewer diabetes patients with physical activity have no significant impact on lowering RBG levels, compared to inactive BQ chewer patients. B) Family history of diabetes significantly impacts the RBG level of BQ chewers and non-chewers, determined by student-t test. C) Uninformed BQ chewers have a tendency to be diagnosed later than non-chewers, which has a significant influence on diabetes management. Glucose levels are in mg/dl, represented as mean + SEM.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/30/178-1716310832-Figure4.jpg",
"caption": "Figure 4. Evaluation of representative performance model of Dry nuts. A) The ROC curve analysis revealed an AUC of 0.726 with a 95% CI from 0.698 to 0.754, indicating a statistically significant model performance (p < 0.0001). The model effectively distinguishes between \"dry nut\" and \"other\" categories, with substantial discriminatory power. B) The classification table shows an 87.27% correct classification rate for the \"other\" category and a 48.90% rate for \"dry nut,\" resulting in an overall accuracy of 71.39%. The model performs better for the \"other\" category.",
"featured": false
}
],
"authors": [
{
"id": 1409,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Abdullah Al",
"family_name": "Marzan",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-6235-2277",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 293
},
{
"id": 1410,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Md. Soyib",
"family_name": "Hasan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 293
},
{
"id": 1411,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Md. Rafiul",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 293
},
{
"id": 1412,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Miah Mohammad",
"family_name": "Sakib",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 293
},
{
"id": 1413,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Md. Sifatul",
"family_name": "Islam",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 293
},
{
"id": 1415,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Md Sakil",
"family_name": "Arman",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-1810-0056",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 293
},
{
"id": 1417,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Md. Rakibul",
"family_name": "Islam",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 293
},
{
"id": 1414,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Mohammad Abul",
"family_name": "Hasnat",
"email": null,
"author_order": 8,
"ORCID": "http://orcid.org/0000-0002-5644-2671",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 293
},
{
"id": 1416,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Zafrul",
"family_name": "Hasan",
"email": "zafrul-bmb@sust.edu",
"author_order": 9,
"ORCID": "https://orcid.org/0000-0001-5914-8411",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Zafrul Hasan, PhD; Department of Biochemistry and Molecular Biology Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh\r\nEmail: zafrul-bmb@sust.edu",
"article": 293
}
]
}
]
},
{
"id": 22,
"title": "Vol7 Issue2",
"cover": "https://jabet.bsmiab.org/media/issue_cover/Jabet_Coverpage_OEIe4hV.jpg",
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2024",
"slug": "2024-vol7-issue2",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 292,
"slug": "178-1709021572-human-mesenchymal-stem-cell-secretome-increases-hepatocyte-growth-factor-expression-and-promotes-liver-regeneration-in-wistar-rats-with-cholestasis",
"type": "original_article",
"manuscript_id": "178-1709021572",
"published": "2024-05-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/58/178-1709021572.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 451-459",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Devi M, Susilaningsih M, et al. Human mesenchymal stem cell secretome increases hepatocyte growth factor expression and promotes liver regeneration in Wistar rats with cholestasis. J Adv Biotechnol Exp Ther. 2024; 7(2): 451-459.",
"title": "Human mesenchymal stem cell secretome increases hepatocyte growth factor expression and promotes liver regeneration in Wistar rats with cholestasis",
"abstract": "<p>Globally, the annual mortality rate due to liver cirrhosis is 2.2%, which ranks 12<sup>th</sup> as the cause of death in the United States. Human mesenchymal stem cell secretome (Hu-MSC-S) is known to reduce liver injury and inflammation. Thus, the present study aims to determine the effect of Hu-MSC-S on hepatocyte growth factor (HGF) expression and liver regeneration in cholestatic rats after choledochal duct ligation receiving standard ursodeoxycholic acid (UDCA) therapy. A randomized experimental study was conducted on 24 male Wistar rats. All rats were randomly assigned into four groups such as control, UDCA, Hu-MSC-S, and UDCA+Hu-MSC-S. The expression of HGF and mitotic index as markers of liver regeneration were evaluated using histopathologic examination. Both HGF expression and the mitotic index were significantly higher in the UDCA and Hu-MSC-S groups (P < 0.05) compared to the control group. The combined administration of UDCA and Hu-MSC-S resulted in significantly higher HGF expression and mitotic index than the individual intervention (P < 0.05). The additional administration of Hu-MSC-S increased HGF expression and mitotic index in rat model of hepatic cholestasis. In conclusion, HuMSC-S administration could be considered as a therapy in patients who received standard UDCA therapy for liver regeneration. <strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d39",
"views": 1205,
"downloads": 231,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/12/28/178-1709021572-Figure1.jpg",
"caption": "Figure 1. Immunohistochemistry of HGF expression (A) and microscopic examination of the mitotic index with hematoxylin-eosin (HE) staining (B). The arrow show HGF expression (A) and mitotic index (B). Control group (Cholestasis), UDCA group (UDCA+Cholestasis), Hu-MSC-S group (HU-MSC-S+Cholestasis), the combination of UDCA and HuMSC-S group (UDCA + Hu-MSC-S+Cholestasis).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/28/178-1709021572-Figure2.jpg",
"caption": "Figure 2. The boxplot graphic of HGF expression in each group. Control group (Cholestasis), UDCA group (UDCA+Cholestasis), Hu-MSC-S group (HU-MSC-S+Cholestasis), the combination of UDCA and HuMSC-S group (UDCA + Hu-MSC-S+Cholestasis). A statistically significant difference was also found when comparing each group. Asterisk (*) means it reached statistical significance (P < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/28/178-1709021572-Figure3.jpg",
"caption": "Figure 3. The boxplot graphic of the mitotic index in each group. Control group (Cholestasis), UDCA group (UDCA+Cholestasis), Hu-MSC-S group (HU-MSC-S+Cholestasis), the combination of UDCA and HuMSC-S group (UDCA + Hu-MSC-S+Cholestasis). Asterisk (*) means it reached statistical significance (P < 0.05).",
"featured": false
}
],
"authors": [
{
"id": 1403,
"affiliation": [
{
"affiliation": "Department of Surgery, Faculty of Medicine Diponegoro University, Dr.Kariadi General Hospital, Semarang, Indonesia"
}
],
"first_name": "Mariani",
"family_name": "Devi",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 292
},
{
"id": 1404,
"affiliation": [
{
"affiliation": "Department of Anatomy Histology, Faculty of Medicine Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Neni",
"family_name": "Susilaningsih",
"email": "nsusilaningsih@gmail.com",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Neni Susilaningsih, Department of Anatomy Histology, Faculty of Medicine Diponegoro University, Semarang, Indonesia. Email: nsusilaningsih@gmail.com",
"article": 292
},
{
"id": 1405,
"affiliation": [
{
"affiliation": "Department of Surgery, Faculty of Medicine Diponegoro University, Dr Kariadi General Hospital, Semarang, Indonesia"
}
],
"first_name": "Parish",
"family_name": "Budiono",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 292
},
{
"id": 1406,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutic, Faculty of Medicine Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Nani",
"family_name": "Maharani",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 292
},
{
"id": 1407,
"affiliation": [
{
"affiliation": "Department of Surgery, Faculty of Medicine Diponegoro University, Dr Kariadi General Hospital, Semarang, Indonesia"
}
],
"first_name": "Erik",
"family_name": "Prabowo",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 292
},
{
"id": 1408,
"affiliation": [
{
"affiliation": "Department of Surgery, Faculty of Medicine Diponegoro University, Dr Kariadi General Hospital, Semarang, Indonesia"
}
],
"first_name": "Ignatius",
"family_name": "Riwanto",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 292
}
]
},
{
"id": 291,
"slug": "178-1706248076-a-comparative-retrospective-study-on-syphilis-serology-in-hiv-positive-and-negative-individuals-before-and-after-therapy",
"type": "original_article",
"manuscript_id": "178-1706248076",
"published": "2024-05-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/43/178-1706248076.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 442-450",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Rahmatika AS, Hidayati AN, et al. A comparative retrospective study on syphilis serology in HIV-positive and negative individuals before and after therapy. J Adv Biotechnol Exp Ther. 2024; 7(2): 442-450.",
"title": "A comparative retrospective study on syphilis serology in HIV-positive and negative individuals before and after therapy",
"abstract": "<p>Syphilis serological tests are crucial for diagnosing and evaluating syphilis therapy. Human immunodeficiency virus (HIV) infection can alter syphilis serologies, making manifestations more atypical and increasing complications. This study aims to compare treponemal and nontreponemal titers before and after therapy between HIV-positive and negative patients. From January 2018 to December 2021, this study retrospectively evaluated patients diagnosed with early and latent syphilis using quantitative nontreponemal and treponemal serologies (VDRL and TPHA) and categorized them as HIV-positive or negative. Baseline and post-therapy (1 month and last follow-up within 2 years) VDRL and TPHA titers were recorded. Twenty-nine HIV-negative and 28 HIV-positive patients met the inclusion criteria. Baseline VDRL and TPHA titers differed significantly between both groups, particularly in early syphilis, with the median baseline VDRL titer higher in the HIV-positive group. At 1-month and the last follow-up after therapy, VDRL titers continued to differ significantly, underscoring the impact of HIV coinfection on treatment response. Notably, TPHA titers exhibited significant differences between the two groups only at the last follow-up in overall syphilis. The HIV-positive group showed a higher percentage of patients failing to achieve a serological cure (60.7% vs. 58.6%). HIV coinfection in syphilis patients affects both nontreponemal and treponemal titers and elevates the treatment failure risk. This study highlights the importance of periodic syphilis serology tests in coinfected individuals to ensure treatment effectiveness, detect reinfection promptly, and prevent complications.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d38",
"views": 1250,
"downloads": 228,
"figures": [],
"authors": [
{
"id": 1391,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Amira Suryani",
"family_name": "Rahmatika",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0005-0399-1692",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1392,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
},
{
"affiliation": "Universitas Airlangga Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Afif Nurul",
"family_name": "Hidayati",
"email": "afif_nurulhidayati@fk.unair.ac.id",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-2558-2046",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Afif Nurul Hidayati, Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia, Email: afif_nurulhidayati@fk.unair.ac.id",
"article": 291
},
{
"id": 1393,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Cita Rosita Sigit",
"family_name": "Prakoeswa",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-3232-095X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1394,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Muhammad Yulianto",
"family_name": "Listiawan",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0001-7810-887X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1395,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Rahmadewi",
"family_name": null,
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1396,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Damayanti",
"family_name": null,
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1397,
"affiliation": [
{
"affiliation": "Department of Public Health Sciences, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Budi",
"family_name": "Utomo",
"email": null,
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-1147-3263",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1398,
"affiliation": [
{
"affiliation": "Department of Public Health Sciences, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Dwi",
"family_name": "Murtiastutik",
"email": null,
"author_order": 8,
"ORCID": "https://orcid.org/0000-0002-5486-5291",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1399,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Astindari",
"family_name": null,
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1400,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Maylita",
"family_name": "Sari",
"email": null,
"author_order": 10,
"ORCID": "https://orcid.org/0000-0002-0866-6355",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1401,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Septiana",
"family_name": "Widyantari",
"email": null,
"author_order": 11,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
},
{
"id": 1402,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Public Academic Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "Regitta Indira",
"family_name": "Agusni",
"email": null,
"author_order": 12,
"ORCID": "https://orcid.org/0000-0001-8196-1505",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 291
}
]
},
{
"id": 290,
"slug": "178-1709839856-association-of-neutrophils-monocytes-and-lymphocytes-with-ca15-3-as-a-predictor-of-breast-cancer-in-female-patients",
"type": "original_article",
"manuscript_id": "178-1709839856",
"published": "2024-05-15",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/21/178-1709839856.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 433-441",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Madhi R, Jumaah HA, et al. Association of neutrophils, monocytes, and lymphocytes with CA15-3 as a predictor of breast cancer in female patients. J Adv Biotechnol Exp Ther. 2024; 7(2): 433-441",
"title": "Association of neutrophils, monocytes, and lymphocytes with CA15-3 as a predictor of breast cancer in female patients",
"abstract": "<p>Infiltrated leukocytes, such as neutrophils, monocytes, and lymphocytes can be involved in a variety of tumor development. The present study aimed to investigate the linkage of neutrophils, monocytes, and lymphocytes with CA15-3 as a predictor of breast cancer. The study included 116 patients (58 patients before chemotherapy and 58 patients after chemotherapy. CA15-3 antigen levels were measured, and the grading of breast cancer was identified. In addition, neutrophils, monocytes, and lymphocytes percentage and their correlation to CA15-3 antigen were determined. The results observed a significant increase in the CA15-3 antigen levels. Moreover, the study also showed that CA15-3 antigen levels have a great correlation with grades of breast cancer particularly in grade 3 before treatment. The results also found that neutrophils and monocyte percentages were significantly increased in patients before treatment. However, lymphocyte percentage decreased before treatment and greatly increased after treatment. Additionally, the results showed a positive correlation between neutrophil and monocytes percentage with CA15-3 antigen levels before and after treatment. However, the correlation was negative between lymphocyte percentage and CA15-3 antigen levels. The present findings observed that neutrophils and monocytes have a great association with CA15-3 antigen levels, therefore, targeting these cells could protect against breast cancer development. However, targeting these cells can negatively affect the patient's immune response, thus further studies are needed to study the exact mechanism by which neutrophils and monocytes could enhance breast cancer development. Additionally, increased lymphocytes after treatment might be a good strategy to treat breast cancer. <strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d37",
"views": 1340,
"downloads": 132,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/16/178-1709839856-Figure1.jpg",
"caption": "Figure 1. CA15-3 antigen levels. ELISA method was used to measure the levels of CA15-3 antigen in control and patients with breast cancer before and after getting the chemotherapy. Patients with breast cancer are introduced by the black box. Control groups are introduced by the grey box. Data introduced as means ± SEM. #P <0.05 versus control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/16/178-1709839856-Figure2.jpg",
"caption": "Figure 2. Breast cancer grading and CA15-3 antigen correlation. Sectioning of (H&E) of breast tissue. Scale bar = 50 μm. A) Normal breast tissue, B) Grade 1, C) Grade 2, and D) Grade 3. CA15-3 antigen association into breast cancer grading, E) before getting chemotherapy and F) after getting chemotherapy. Data represented as mean of percentage; the LDS method was used to compare between groups. *P-value <0.05 is considered as a significant difference between the groups.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/16/178-1709839856-Figure3.jpg",
"caption": "Figure 3. Estimation of neutrophils, monocytes, and lymphocytes in the blood. Measurement percentage of A) neutrophils, B) monocytes, and C) lymphocytes in both control and patient groups. Control group (gray box). Patients with breast cancer before and after getting the chemotherapy (black box). Data represent means ± SEM. *A p-value of <0.05 is considered a significant difference between the groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/16/178-1709839856-Figure4.jpg",
"caption": "Figure 4. Linkage of neutrophils, monocytes, and lymphocytes with CA15-3 antigen levels. Correlation of CA15-3 antigen levels into neutrophil A) before getting chemotherapy (y = 0.981x + 52.352, R² 0.7204 ) and B) after getting chemotherapy (y = 3.2024x - 22.109, R² = 0.7512), monocytes C) before getting chemotherapy (y = 0.2342x + 2.3657, R² = 0.5628) and D) after getting chemotherapy (y = -0.6086x + 16.817, R² = 0.7486), and lymphocytes E) before getting chemotherapy (y = 0.1195x + 11.458, R² = 0.0502) and F) after getting chemotherapy (y = -0.0776x + 48.993, R² = 0.0011). Linear Regression was used to compare between groups.",
"featured": false
}
],
"authors": [
{
"id": 1388,
"affiliation": [
{
"affiliation": "Department of Biology, Collage of Science, University of Misan, 62001 Maysan, Iraq"
}
],
"first_name": "Raed",
"family_name": "Madhi",
"email": "raedsaddam@uomisan.edu.iq",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-7034-646X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Raed Madhi, PhD; Department of Biology, Collage of Science, University of Misan, Maysan, Iraq. Email: raedsaddam@uomisan.edu.iq",
"article": 290
},
{
"id": 1389,
"affiliation": [
{
"affiliation": "Al-Hussein Teaching Hospital, Thi-Qar Health Directorate, 64001 Thi-Qar, Iraq"
}
],
"first_name": "Hussein Ali",
"family_name": "Jumaah",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 290
},
{
"id": 1390,
"affiliation": [
{
"affiliation": "Al-Hussein Teaching Hospital, Thi-Qar Health Directorate, 64001 Thi-Qar, Iraq"
}
],
"first_name": "Hasan Hanoon",
"family_name": "Hasan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 290
}
]
},
{
"id": 289,
"slug": "178-1709698463-impact-of-seaweed-on-growth-performance-sperm-quality-and-testicular-histomorphology-of-ram",
"type": "original_article",
"manuscript_id": "178-1709698463",
"published": "2024-05-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/52/178-1709698463.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 420-432",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Hoque SAM, Selim ASM, et al. Impact of seaweed on growth performance, sperm quality, and testicular histomorphology of ram. J Adv Biotechnol Exp Ther. 2024; 7(2): 420-432",
"title": "Impact of seaweed on growth performance, sperm quality, and testicular histomorphology of ram",
"abstract": "<p>Sperm quality is critical for male fertility, which is largely dependent on testicular development; however, nutrition is critical for optimal growth, gonadal development, and reproductive functions. This study aimed to investigate the effects of <em>Gracilaria parvispora</em>, a red seaweed on growth performance, testicular physiology, and semen quality in ram. Fifteen indigenous healthy rams, weighing 10-11 kg and aged 12-13 months, were randomly allocated into three groups. One group was considered as control and supplied with a German grass and concentrate mixture (control diet). The other two groups were provided with a control diet plus seaweed mixture at 7 g/d or 14 g/d. The starting body weight, scrotal circumferences, and semen quality of rams were similar for all groups. However, post-treatment, both the seaweed-treated groups showed significantly higher body weight, total weight gain, daily weight gain, and increased scrotal circumference and testicular dimensions, compared to the control group. Moreover, the semen parameters, including sperm concentration, live sperm percentage, mass motility, and progressive motility were observed to be significantly increased, while decreasing abnormal sperm percentage in the seaweed-treated group. Furthermore, DPPH and ABTS scavenging abilities of sperm and <em>in vitro</em> fertilization rate were increased dramatically in seaweed-treated groups. Additionally, the testicular histology of seaweed-treated groups revealed a thinner tunica albuginea with expanded seminiferous tubules containing a higher number of spermatogenic cells inside. These findings suggest that seaweed <em>G. parvispora</em> could be a valuable dietary supplement for enhancing the growth and reproductive performance of ram. <strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d36",
"views": 1849,
"downloads": 202,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/07/13/178-1709698463-Figure1.jpg",
"caption": "Figure 1. The semen volume and sperm quality parameters of ram treated with or without seaweed. Where (A) indicates Semen volume, (B) Sperm concentration, (C) Percentage of abnormal sperm, (D) Percentage of live sperm, (E) Sperm total motility and (F) Sperm progressive motility. Values are Mean ± SD of five replicates. Different lowercase letters superscripts (a or b) in the same week or duration (rectangular boxes) indicates the significant (p<0.05) differences among the treatments. C – Control; SW 7g/d – Seaweed treated group (7g/d/head); SW 14g/d – Seaweed treated group (14g/d/head).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/07/13/178-1709698463-Figure2.jpg",
"caption": "Figure 2. The radical scavenging activity and fertilizing ability of sperm. Where (A) indicates sperm DPPH scavenging activity, (B) ABTS scavenging activity, and (C) Rate of In vitro fertilization. Values are Mean ± SD of five replicates. Different lowercase letters on the bar (a or b) indicate the significant (p<0.05) difference among the treatments. C – Control; SW 7g/d – Seaweed treated group (7g/d/head); SW 14g/d – Seaweed treated group (14g/d/head).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/07/13/178-1709698463-Figure3.jpg",
"caption": "Figure 3. The histological sections and the thickness of tunica albuginea of testis. Where (A) indicates testicular morphology showing the tunica albuginea, (B) the thickness of tunica albuginea. Values are Mean ± SD of five replicates of the average of both right and left testis. Different lowercase letters on the bar (a or b) indicate the significant (p<0.05) difference among the treatments. C – Control; SW 7g/d – Seaweed treated group (7g/d/head); SW 14g/d – Seaweed treated group (14g/d/head). Scale bar is 200 µm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/02/178-1709698463-Figure4.jpg",
"caption": "Figure 4. The histological sections show the seminiferous tubules of testis. Where (A) indicates morphology of seminiferous tubules, (B) the length of seminiferous tubules, (C) the breadth of seminiferous tubules and (D) the number of spermatogenic cells in the seminiferous tubules. Values are Mean ± SD of five replicates of the average of both right and left testis. Different lowercase letters on the bar (a or b) indicate the significant (p<0.05) difference among the treatments. C – Control; SW 7g/d – Seaweed treated group (7g/d/head); SW 14g/d – Seaweed treated group (14g/d/head). The scale bar is 100 µm.",
"featured": true
}
],
"authors": [
{
"id": 1382,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
},
{
"affiliation": "Institute of Climate Change and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "S. A. Masudul",
"family_name": "Hoque",
"email": "mhoqueabg@bsmrau.edu.bd",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-3537-5892",
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "S. A. Masudul Hoque, Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh. Email: mhoqueabg@bsmrau.edu.bd",
"article": 289
},
{
"id": 1383,
"affiliation": [
{
"affiliation": "Department of Animal Science and Nutrition, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
},
{
"affiliation": "Institute of Climate Change and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Abu Sadeque Md.",
"family_name": "Selim",
"email": "asmselim@bsmrau.edu.bd",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-8457-4631",
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "Abu Sadeque Md. Selim, PhD, Department of Animal Science and Nutrition, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh, Email: asmselim@bsmrau.edu.bd",
"article": 289
},
{
"id": 1384,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Md. Mazharul",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 289
},
{
"id": 1385,
"affiliation": [
{
"affiliation": "Department of Animal Science and Nutrition, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Md. Rashidul",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 289
},
{
"id": 1386,
"affiliation": [
{
"affiliation": "Department of Animal Science and Nutrition, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Israt Jahan",
"family_name": "Meem",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 289
},
{
"id": 1387,
"affiliation": [
{
"affiliation": "Department of Dairy and Poultry Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Md. Morshedur",
"family_name": "Rahman",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 289
}
]
},
{
"id": 288,
"slug": "178-1706500032-effects-of-catfish-oil-and-curcuma-longa-l-extract-on-apob-levels-and-atherosclerosis-risk-indicators-in-metabolic-syndrome-rats",
"type": "original_article",
"manuscript_id": "178-1706500032",
"published": "2024-05-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/17/178-1706500032.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 408-419",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Pramayastri V, Mahati E, et al. Effects of catfish oil and Curcuma longa L. extract on ApoB levels and atherosclerosis risk indicators in metabolic syndrome rats. J Adv Biotechnol Exp Ther. 2024; 7(2): 408-419.",
"title": "Effects of catfish oil and <span>Curcuma longa</span> L. extract on ApoB levels and atherosclerosis risk indicators in metabolic syndrome rats",
"abstract": "<p>The increase in ApoB levels, atherogenic index of plasma (AIP), and Castelli risk index I-II (CRI) involves cardiovascular disease in metabolic syndrome. Lipid peroxidation could initiate the process of atherosclerosis and involve in the etiology of cardiovascular disease. Dietary fish oil and extract of <em>Curcuma longa </em>Linn. have been shown to reduce lipid peroxidation and atherosclerosis. The aim of this study was to determine the effect of catfish oil and <em>Curcuma </em>extract on ApoB levels and lipid ratios as protective nutraceutical for metabolic syndrome. Thirty male Wistar<em> </em>rats were randomly divided into 5 groups namely healthy control (HC), high-fat high-fructose diet (HFFD), and intervention group P1 (HFFD + catfish oil 0.08 ml/g/day), P2 (HFFD + <em>Curcuma </em>extract 5.04 mg/g/day), and P3 with HFFD + combination of both. HFFD induced metabolic syndrome in rats. ApoB levels were measured by the immunoturbidimetric method and lipid levels with ELISA. Lipid ratios were calculated by AIP (log TG/HDL), and the CRI I-II (TC/HDL, LDL/HDL). The study was showed significantly reduced in ApoB levels, AIP, and CRI in intervention groups. The correlation test results showed that a significant correlation among ApoB levels, AIP, and CRI. In conclusion, combination of both may decrease levels of ApoB and atherosclerosis risk indicators in metabolic syndrome rats.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d35",
"views": 1084,
"downloads": 159,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/12/178-1706500032-Figure1.jpg",
"caption": "Figure 1. Effects of intervention (pre-and post-treatments) on ApoB levels. According to the results of the one-way ANOVA test, the data variants were homogenous, and the data of ApoB levels were significantly different indicated by # compared to the HC group. Significant differences are indicated by * when compared with the HFFD group. HC (healthy control); HFFD (negative control); and intervention groups (P1, P2, and P3); n=6; *= p<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/12/178-1706500032-Figure2.jpg",
"caption": "Figure 2. Effects of intervention (pre-and post-treatments) on AIP. The result of the Kruskall-Wallis test resulted in p=0.000 (<0.05). This demonstrates that the data was significantly different and indicated by # compared to the HC group. Significant differences are indicated by * when compared with the HFFD group. HC (healthy control), HFFD (negative control), and intervention groups (P1, P2, and P3), n=6; *= p<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/12/178-1706500032-Figure3.jpg",
"caption": "Figure 3. Effects of intervention (pre-and post-treatments) on CRI-I. According to the results of one-way ANOVA test, the data variants were homogenous, and the data of CRI-I had significantly different are indicated by # compared to the HC group. Significant differences are indicated by * when compared with the HFFD group. HC (healthy control), HFFD (negative control), and intervention groups (P1, P2, and P3), n=6; *= p<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/12/178-1706500032-Figure4.jpg",
"caption": "Figure 4. Effects of intervention (pre-and post-treatments) on CRI-II. According to the results of one-way ANOVA test, the data variants were homogenous, and the data of CRI-II had significantly different are indicated by # compared to the HC group. Significant differences are indicated by * when compared with the HFFD group. HC (healthy control), HFFD (negative control), and intervention groups (P1, P2, and P3), n=6; *= p<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/12/178-1706500032-Figure5.jpg",
"caption": "Figure 5. Linear regression of ApoB levels with AIP.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/12/178-1706500032-Figure6.jpg",
"caption": "Figure 6. Linear regression of ApoB levels with CRI-I.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/12/178-1706500032-Figure7.jpg",
"caption": "Figure 7. Linear regression of ApoB levels with CRI-II.",
"featured": false
}
],
"authors": [
{
"id": 1373,
"affiliation": [
{
"affiliation": "Department of Nutrition, Faculty of Medicine, Universitas Diponegoro, Semarang, Central Java, Indonesia"
}
],
"first_name": "Vina",
"family_name": "Pramayastri",
"email": "pramayastri@gmail.com",
"author_order": 1,
"ORCID": "http://orcid.org/0009-0009-4028-8727",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Vina Pramayastri, Department of Nutrition, Faculty of Medicine, Universitas Diponegoro, Semarang, Central Java, Indonesia. Email: pramayastri@gmail.com",
"article": 288
},
{
"id": 1374,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Diponegoro, Semarang, Central Java, Indonesia"
}
],
"first_name": "Endang",
"family_name": "Mahati",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-9101-8753",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 288
},
{
"id": 1375,
"affiliation": [
{
"affiliation": "Department of Nutrition, Faculty of Medicine, Universitas Diponegoro, Semarang, Central Java, Indonesia"
}
],
"first_name": "Mohammad",
"family_name": "Sulchan",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-6961-2780",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 288
},
{
"id": 1376,
"affiliation": [
{
"affiliation": "Department of Nutrition, Faculty of Medicine, Universitas Diponegoro, Semarang, Central Java, Indonesia"
},
{
"affiliation": "Center of Nutrition Research (CENURE), Univeristas Diponegoro, Semarang, Indonesia"
},
{
"affiliation": "SDGs Center, Universitas Diponegoro, Semarang, Indonesia"
}
],
"first_name": "Diana Nur",
"family_name": "Afifah",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0001-8808-1826",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 288
}
]
},
{
"id": 287,
"slug": "178-1708276471-immunomodulatory-effect-of-aqueous-extract-of-termitomyces-striatus-mushroom-on-enterohemorrhagic-escherichia-coli-infected-mice",
"type": "original_article",
"manuscript_id": "178-1708276471",
"published": "2024-05-09",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/49/178-1708276471.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 396-407",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Sitati CNW, Ogila KO, et al. Immunomodulatory effect of aqueous extract of Termitomyces striatus mushroom on enterohemorrhagic Escherichia coli-infected mice. J Adv Biotechnol Exp Ther. 2024; 7(2): 396-407",
"title": "Immunomodulatory effect of aqueous extract of <span>Termitomyces striatus</span> mushroom on enterohemorrhagic <span>Escherichia coli</span>-infected mice",
"abstract": "<p>Enterohemorrhagic <em>Escherichia coli</em> (EHEC) generates Shiga toxins that cause immunosuppression and diarrhea. Synthetic immunomodulatory drugs are associated with numerous severe effects, necessitating the need for substitute therapeutic agents. This study aimed to determine the immunomodulatory activity of aqueous extract of <em>Termitomyces striatus</em> in mice infected with EHEC. Mice in the positive control, negative control, and two extract-treated groups were administered orally with 200µL of 9×10<sup>8</sup> CFU/mL EHEC. From day 5, the positive control, negative control, and extract-treated mice were orally administered with levamisole (50mg/kg body weight (bw)), phosphate-buffered saline (vehicle), and aqueous extract of <em>T. striatus</em> (200 and 400mg/kg body weight), respectively. The control mice received the vehicle only. After the experiment, the mice were euthanized, blood was collected, and white blood cells (WBCs) and differentials were analyzed using a hematological analyzer. Immunoglobulin G (IgG), interleukin-4 (IL-4), and interferon-gamma (INF-γ) levels were also quantified using enzyme-linked immunosorbent assays. The extract significantly reduced elevated levels of WBCs, neutrophils, monocytes, eosinophils, and basophils, as well as significantly enhanced the levels of lymphocytes in EHEC-infected mice (p<0.05). Besides, the extract significantly lowered the levels of INF-γ while significantly increased the levels of IL-4 and IgG in EHEC-infected mice. Flavonoids, alkaloids, saponins, steroids, and phenolics are some of the phytochemicals that were detected in <em>T. striatus</em>. Together, these findings revealed that <em>T. striatus</em> extract modulated immune response in EHEC-challenged mice by lowering leucocyte counts and INF-γ, enhancing lymphocytes, IL-4, and IgG, suggesting that the extract could be developed as an immunomodulatory agent.</p>",
"DOI": "10.5455/jabet.2024.d34",
"views": 1195,
"downloads": 152,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/14/12/178-1708276471-Figure1.jpg",
"caption": "Figure 1. Effects of Termitomyces striatus aqueous extract on total leukocytes and differential counts in EHEC-infected mice. Bars with distinct letters on the same day differ statistically (p<0.05) using one-way ANOVA and Bonferroni multiple comparisons.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/14/12/178-1708276471-Figure2.jpg",
"caption": "Figure 2. Effects of Termitomyces striatus aqueous extract on levels of INF-γ in EHEC-infected mice. Bars with distinct lowercase letters on the same day differ statistically (p<0.05) using one-way ANOVA and Bonferroni multiple comparisons.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/14/12/178-1708276471-Figure3.jpg",
"caption": "Figure 3. Effects of Termitomyces striatus aqueous extract on levels of IL-4 in EHEC-infected mice. Bars with distinct lowercase letters on the same day differ statistically (p<0.05) using one-way ANOVA and Bonferroni multiple comparisons.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/14/12/178-1708276471-Figure4.jpg",
"caption": "Figure 4. Effects of Termitomyces striatus aqueous extract on levels of IgG in EHEC-infected mice. Bars with distinct lowercase letters on the same day differ statistically (p<0.05) using one-way ANOVA and Bonferroni multiple comparisons.",
"featured": false
}
],
"authors": [
{
"id": 1369,
"affiliation": [
{
"affiliation": "Natural Sciences Department, School of Pure and Applied Sciences, Mount Kenya University, Thika, Kenya"
},
{
"affiliation": "Zoology Department, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi Kenya"
}
],
"first_name": "Concepta Nekesa W.",
"family_name": "Sitati",
"email": "csitati@mku.ac.ke",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-7478-7297",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Concepta Nekesa W. Sitati, Department of Natural Sciences, Mount Kenya University, PO BOX 342-01000, Thika, Kenya. Email: csitati@mku.ac.ke",
"article": 287
},
{
"id": 1370,
"affiliation": [
{
"affiliation": "Zoology Department, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi Kenya"
}
],
"first_name": "Kenneth Omondi",
"family_name": "Ogila",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-3381-2473",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 287
},
{
"id": 1371,
"affiliation": [
{
"affiliation": "Zoology Department, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi Kenya"
}
],
"first_name": "Rebecca Wanjiku",
"family_name": "Waihenya",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-4629-3481",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 287
},
{
"id": 1372,
"affiliation": [
{
"affiliation": "Department of Tropical and Infectious Diseases, Institute of Primate Research, Nairobi, Kenya"
}
],
"first_name": "Lucy A.",
"family_name": "Ochola",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0003-0264-5526",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 287
}
]
},
{
"id": 286,
"slug": "178-1704599281-hyperbaric-oxygen-as-an-immunosuppressant-in-mouse-model-of-lupus-nephritis",
"type": "original_article",
"manuscript_id": "178-1704599281",
"published": "2024-05-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/40/178-1704599281.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 385-395",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Harnanik T, Yudadi R, et al. Hyperbaric oxygen as an immunosuppressant in mouse model of lupus nephritis. J Adv Biotechnol Exp Ther. 2024; 7(2): 385-395.",
"title": "Hyperbaric oxygen as an immunosuppressant in mouse model of lupus nephritis",
"abstract": "<p>Hyperbaric oxygen (HBO) therapy has been used for many conditions. The immunosuppressive effect of HBO is also reported. Thus, HBO is thought to be used for the treatment of lupus nephritis (LN). The current research intended to determine the effect of HBO 2.0 ATA by inhaling ±100% oxygen (O<sub>2</sub>) 3 times for 30 min, for 10 consecutive days as an immunosuppressant in an animal model of LN. A total of 21 BALB/c female healthy and active mice (body weight 25-30 g and 8-12 weeks old) were divided into 3 groups including the group of control mice (G1), the group of LN mice without HBO therapy (G2), and the group of LN mice with HBO therapy (G3). The results showed a significant reduction of anti-dsDNA Ab and proteinuria levels in the HBO (G3) compared to the LN group not given HBO (G2). Repair of kidney tissue damage was characterized by a decrease in inflammatory cells, a decrease in tubular constriction and a decrease in immune complex deposits in the group given HBO (G3) compared to the group not given HBO (G2). HBO can reduce anti-dsDNA Ab levels and proteinuria, and repair damaged kidney tissue. In conclusion, the findings suggest the HBO as an immunosuppressive in LN in mice.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d33",
"views": 996,
"downloads": 234,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/12/178-1704599281-Figure1.jpg",
"caption": "Figure 1. A) Group of experimental mice in the cages. B) Mice were injected with Pristane intra-peritoneally.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/12/178-1704599281-Figure2.jpg",
"caption": "Figure 2. A) Hyperbaric animal chamber. B) The process of inserting experimental treatment group animal models into the hyperbaric animal chamber. C) The treatment group animal model received oxygen therapy in a hyperbaric animal chamber.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/12/178-1704599281-Figure3.jpg",
"caption": "Figure 3. Diagram of differences in mean anti-dsDNA Ab levels in groups G1, G2, and G3. The average value of ab anti-dsDNA levels in those who received HBO (G3) decreased significantly compared to those who did not receive HBO (G2). G1= control group; G2 = LN + non-HBO group; and G3 = LN + HBO group. * Indicates a significant difference (p<0.05), *on G2 vs G1, and * on G3 vs G2.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/12/178-1704599281-Figure4.jpg",
"caption": "Figure 4. Diagram of differences in mean proteinuria levels in groups G1, G2, and G3. The average value of proteinuria levels in those who received HBO (G3) decreased significantly compared to those who did not receive HBO (G2). G1= control group; G2 = LN + non-HBO group; and G3 = LN + HBO group. * Indicates a significant difference (p<0.05), *on G2 vs G1, and * on G3 vs G2.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/12/178-1704599281-Figure5.jpg",
"caption": "Figure 5. Expression of changes in kidney tissue in groups G1, G2, and G3 by a histochemical technique using a light microscope, shown at 400x magnification (200µm). A: Inflammatory cells appear swollen with an enlarged cytoplasmic volume. B: constricted tubules. C: deposits of immune complexes. In the group given HBO therapy (G3), there was an improvement in kidney damage which was characterized by a decrease in inflammatory cells, a decrease in tubular constriction, and a decrease in immune complex deposits compared to the group not given HBO therapy (G2). G1= control group; G2 = LN + non-HBO group; and G3 = LN + HBO group.",
"featured": true
}
],
"authors": [
{
"id": 1377,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Medicine, Hang Tuah University, Gadung 1, 60244 Surabaya, East Java, Indonesia"
},
{
"affiliation": "Naval Health Institute Drs. Med. R. Rijadi S, Phys., Surabaya, Indonesia"
}
],
"first_name": "Titut",
"family_name": "Harnanik",
"email": "titut.harnanik1972@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-1282-7614",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Titut Harnanik; Department of Physiology, Faculty of Medicine, Hang Tuah University, Gadung 1, 60244 Surabaya, East Java, Indonesia. Email: titut.harnanik1972@gmail.com",
"article": 286
},
{
"id": 1378,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Medicine, Hang Tuah University, Gadung 1, 60244 Surabaya, East Java, Indonesia"
}
],
"first_name": "Redemptus",
"family_name": "Yudadi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 286
},
{
"id": 1379,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Medicine, Hang Tuah University, Gadung 1, 60244 Surabaya, East Java, Indonesia"
}
],
"first_name": "Hendrata Erry",
"family_name": "Andisari",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 286
},
{
"id": 1381,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Medicine, Hang Tuah University, Gadung 1, 60244 Surabaya, East Java, Indonesia"
}
],
"first_name": "Levy Pillar",
"family_name": "Wibisono",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 286
},
{
"id": 1380,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Medicine, Hang Tuah University, Gadung 1, 60244 Surabaya, East Java, Indonesia"
}
],
"first_name": "Ludwinna Kezhya",
"family_name": "Astaraini",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 286
}
]
},
{
"id": 285,
"slug": "178-1708424772-an-initial-assessment-of-status-of-post-covid-19-symptoms-in-vietnamese-students",
"type": "short_communication",
"manuscript_id": "178-1708424772",
"published": "2024-04-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/29/178-1708424772.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 378-384",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Chu DT, Thi HV, et at. An initial assessment of status of post-COVID-19 symptoms in Vietnamese students. J Adv Biotechnol Exp Ther. 2024; 7(2): 378-384.",
"title": "An initial assessment of status of post-COVID-19 symptoms in Vietnamese students",
"abstract": "<p>A cross-sectional descriptive study through the online survey was conducted within a month to describe the status of post-COVID-19 symptoms, quality of life, and related factors among Vietnamese students. The questionnaire consists of three main parts: Part 1: Personal information; Part 2: The post-COVID-19 symptoms; and Part 3: Quality of life (EQ-5D). A total of 402 Vietnamese students over 18 years old had complete responses. Surprisingly, up to 78% of the total reported experiencing at least one post-COVID-19 symptom. Of these, the most reported symptoms were neurological (66.17%). Digestive symptoms were relatively uncommon with only 14.18%. Regarding the quality of life, some reported problems with pain and discomfort (25.62%), and anxiety (37.56%). Self-rated health was a related factor to both the status of post-COVID-19 symptoms and quality of life. These findings suggested that more studies are needed to explore potential treatments for alleviating symptoms and improving the quality of life in recovered patients in the future.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d32",
"views": 948,
"downloads": 156,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/11/178-1708424772-Figure1.jpg",
"caption": "Figure 1. The quality of life of the study subjects.",
"featured": false
}
],
"authors": [
{
"id": 1361,
"affiliation": [
{
"affiliation": "Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam"
},
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Dinh-Toi",
"family_name": "Chu",
"email": "chudinhtoi.hnue@mail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dinh-Toi Chu, PhD; Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam, Email: chudinhtoi.hnue@mail.com",
"article": 285
},
{
"id": 1362,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
},
{
"affiliation": "Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Hue Vu",
"family_name": "Thi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 285
},
{
"id": 1363,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
},
{
"affiliation": "Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Yen Vy Nguyen",
"family_name": "Thi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 285
},
{
"id": 1364,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Thuy Duong",
"family_name": "Vu",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 285
},
{
"id": 1365,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Linh Thao",
"family_name": "Tran",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 285
},
{
"id": 1366,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Quoc Cuong",
"family_name": "Bui",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 285
},
{
"id": 1367,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
},
{
"affiliation": "Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Chuc Hoang",
"family_name": "Thi",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 285
},
{
"id": 1368,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
},
{
"affiliation": "Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Thai Van",
"family_name": "Than",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 285
}
]
},
{
"id": 284,
"slug": "178-1705980181-hypoglycemic-effects-of-propolis-extract-on-polycystic-ovarian-syndrome-in-wistar-rats",
"type": "original_article",
"manuscript_id": "178-1705980181",
"published": "2024-03-23",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/07/178-1705980181.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 369-377",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Puspitasari VD, Lestari ES, et al. Hypoglycemic effects of propolis extract on polycystic ovarian syndrome in Wistar rats. J Adv Biotechnol Exp Ther. 2024; 7(2): 369-377.",
"title": "Hypoglycemic effects of propolis extract on polycystic ovarian syndrome in Wistar rats",
"abstract": "<p>Polycystic ovarian syndrome (PCOS) is an endocrinopathy that causes various metabolic syndromes, including insulin resistance in 20-40% of patients. The treatment of the condition is often carried out using metformin, but it has been reported to have different gastrointestinal side effects. Consequently, several studies have recommended the use of propolis as an alternative, which has proven effective in improving insulin resistance in diabetic rats and causes fewer side effects. Thus, the current study aimed to compare the effects of propolis and metformin in treating insulin resistance based on homeostasis model assessment of insulin resistance (HOMA-IR) value. This study used 30 Wistar rats in randomized control trial design. The animals were divided into 5 groups: control, PCOS (PCO), PCOS with metformin (PCO+M), PCOS with propolis extract (PCO+P), and PCOS with metformin and propolis extract (PCO+MP). Subsequently, blood sampling, ovarian tissue sampling, and vaginal smearing were performed after 28 days of treatment. PCO+P and PCO+M groups experienced a significant decrease in testosterone concentration and preantral follicle count compared to PCOS group, but the anovulation cycle was similar. Fasting blood glucose concentration in PCO+MP group showed a significant decrease compared to others. However, fasting insulin concentration and HOMA-IR value in treatment groups did not show any reduction compared to PCOS group. Based on the results, propolis extract and metformin could not improve insulin resistance based on HOMA-IR value. However, propolis extract had better hypoglycaemic effects compared to metformin. In conclusion, the combination of both treatments had the better potential to lower fasting glucose concentration.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d31",
"views": 1278,
"downloads": 240,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/57/25/178-1705980181-Figure1.jpg",
"caption": "Figure 1. Folliculogenesis in experimental groups. Red arrow: primary follicles; green arrow: secondary follicles; blue arrow: tertiary follicles; yellow arrow: de Graaf follicles; orange arrow: luteal corpora; black arrow: atretic follicles. A) Control group; B) PCO group; C) PCO+M group; D) PCO+P group; and E) PCO+MP group. Preantral follicles were primary and secondary follicles, follicles count was presented in Table 1.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/57/25/178-1705980181-Figure2.jpg",
"caption": "Figure 2. Comparison of FBG (fasting blood glucose), FINS (fasting insulin) and HOMA-IR (homeostasis model assessment for insulin resistance index) value between research groups. ###Significant difference (p<0.05)",
"featured": false
}
],
"authors": [
{
"id": 1358,
"affiliation": [
{
"affiliation": "Doctoral Study Program of Medical and Health Science, Universitas Diponegoro, Prof. Soedarto Street, Semarang, Indonesia"
}
],
"first_name": "Vannya Dewi",
"family_name": "Puspitasari",
"email": "vannyadp@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Vannya Dewi Puspitasari, Doctoral Study Program of Medical and Health Science, Universitas Diponegoro, Prof. Soedarto Street, Semarang, Indonesia. Email: vannyadp@gmail.com",
"article": 284
},
{
"id": 1359,
"affiliation": [
{
"affiliation": "Department of Clinical Microbiology, Faculty of Medicine, Universitas Diponegoro, Prof. Soedarto Street, Semarang, Indonesia"
}
],
"first_name": "Endang Sri",
"family_name": "Lestari",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 284
},
{
"id": 1360,
"affiliation": [
{
"affiliation": "Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Diponegoro, Prof.Soedarto Street, Semarang, Indonesia"
}
],
"first_name": "Noor",
"family_name": "Pramono",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 284
}
]
},
{
"id": 283,
"slug": "178-1706293178-exposure-to-tobacco-spannicotiana-tabacumspan-extract-and-cigarette-smoke-induces-hematological-and-histopathological-alterations-in-swiss-albino-mice",
"type": "original_article",
"manuscript_id": "178-1706293178",
"published": "2024-03-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/28/178-1706293178_sTFOryG.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 357-368",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Jannat N, Akter S, et al. Exposure to tobacco (Nicotiana tabacum) extract and cigarette smoke induces hematological and histopathological alterations in Swiss albino mice. J Adv Biotechnol Exp Ther. 2024; 7(2): 357-368.",
"title": "Exposure to tobacco (<span>Nicotiana tabacum</span>) extract and cigarette smoke induces hematological and histopathological alterations in Swiss albino mice",
"abstract": "<p>Tobacco smoking is a widely practiced recreational activity and is associated with numerous health risks. Hence, the present study was conducted on Swiss albino mice to assess the impact of an aqueous extract of tobacco and cigarette smoke on behavioral, hematological, and histopathological parameters of the lungs, liver, and kidney. The mice were divided into control, <em>Tobacco nicotiana</em> (TN), and cigarette smoke (CS) groups (n=6). The TN group received aqueous tobacco orally at a dose of 5 ml/kg BW for 21 days, whereas the CS group was exposed to inhalational commercial cigarette smoke for the same duration. On day 22, blood samples and visceral organs were collected to evaluate hematological and histopathological parameters in euthanized mice. The total erythrocyte count was significantly elevated (P<0.05) in both groups. In contrast to the control group, the total leukocyte count was lower in the CS group (P>0.05) than in the TN group, but the hemoglobin showed no significant changes. Lower body weight, aggressive behavior, and weakness were observed in the treated groups. Histopathologically, the lung revealed emphysematous changes, alveolar septal thickening, inflammatory cell infiltration, congestion, and thrombus formation in pulmonary artery. The liver exhibited hepatocyte degeneration with karyomegaly and karyorrhexis, derangement of the hepatic cords and sinusoids, and central vein congestion. Derangement of renal tubules, edema, and glomerular degeneration were observed in the kidney. These observations lead us to postulate that tobacco and cigarette smoke exert detrimental effects on the structure of some vital organs in the body after direct or indirect uptake. <strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d30",
"views": 1340,
"downloads": 192,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/13/178-1706293178-Figure1.jpg",
"caption": "Figure 1. Schematic diagram showing the administration of aqueous tobacco and cigarette smoke exposure in mice for a period of 3 weeks.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/13/178-1706293178-Figure2.jpg",
"caption": "Figure 2. Effects of tobacco and cigarette smoke on hematological parameters in mice. A: TEC (Total erythrocyte count); B: TLC (Total leucocyte count); C: Hb% (Hemoglobin percentage). Cont: Control; TN: Tobacco nicotiana; CS: Cigarette smoke group. Each group contains six mice (n=6). Results represented in terms of Mean ± SEM. Here, *= Statistically significant at 5% level (P≤0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/13/178-1706293178-Figure3.jpg",
"caption": "Figure 3. Effects of tobacco and cigarette smoke on histopathology of lung in mice. Cont: Control; TN: Tobacco nicotiana; CS: Cigarette smoke group. Each group contains six mice (n=6). A: Section of lung of control mice showing normal histology. B, C: TN group showing emphysematous change (red arrow), thickening of alveolar septa, and infiltration of inflammatory cells (black arrowhead). D, E: CS group also showing alveolar septal thickening, distortion of bronchiolar smooth muscle and congestion (green arrows), and thrombus formation in pulmonary artery (black arrow). Scale bar: 50µm. Stain: Hematoxylin and Eosin (H&E).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/13/178-1706293178-Figure4.jpg",
"caption": "Figure 4. Effects of tobacco and cigarette smoke on histopathology of liver in mice. Cont: Control; TN: Tobacco nicotiana; CS: Cigarette smoke group. Each group contains six mice (n=6). A: Section of liver of control mice showing normal histology. B, C: TN group showing karyomegaly and karyorrhexis (highlighted in black box). D, E: CS group showing disruption of hepatic cords and unusual sinusoids (red arrow), central vein dilatation with congestion (black arrow). CV: Central Vein. Scale bar: 50µm. Stain: Hematoxylin and Eosin (H&E).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/13/178-1706293178-Figure5.jpg",
"caption": "Figure 5. Effects of tobacco and cigarette smoke on histopathology of kidney in mice. Cont: Control; TN: Tobacco nicotiana; CS: Cigarette smoke group. Each group contains six mice (n=6). A: Section of kidney of control mice showing normal histology. B: TN group showing edema in the interstitial tissues (black arrow). C, D: CS group showing disorganized renal corpuscles (C) and obliterated Bowman’s space with unusual glomerular architecture (D). G: Glomerulus. Scale bar: A, B, C: 50µm; D: 20µm. Stain: Hematoxylin and Eosin (H&E).",
"featured": false
}
],
"authors": [
{
"id": 1354,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Nure",
"family_name": "Jannat",
"email": "njannat@bsmrau.edu.bd",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-6738-7719",
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "Nure Jannat, Department of Anatomy & Histology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh. Email: njannat@bsmrau.edu.bd",
"article": 283
},
{
"id": 1355,
"affiliation": [
{
"affiliation": "Department of Pathobiology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Saima",
"family_name": "Akter",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 283
},
{
"id": 1356,
"affiliation": [
{
"affiliation": "Department of Pathobiology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Md Tanveer",
"family_name": "Hasan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 283
},
{
"id": 1357,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Md Abdullah Al",
"family_name": "Mahmud",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 283
}
]
},
{
"id": 282,
"slug": "178-1700990889-association-of-monocyte-to-lymphocyte-ratio-neutrophil-to-lymphocyte-ratio-and-tumor-necrosis-factor-a-in-various-stages-of-chronic-kidney-disease",
"type": "original_article",
"manuscript_id": "178-1700990889",
"published": "2024-03-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/54/178-1700990889.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 346-356",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Sari DI, Wardhani P, et al. Association of monocyte-to-lymphocyte ratio, neutrophil-to-lymphocyte ratio, and tumor necrosis factor-α in various stages of chronic kidney disease. J Adv Biotechnol Exp Ther. 2024; 7(2): 346-356.",
"title": "Association of monocyte-to-lymphocyte ratio, neutrophil-to-lymphocyte ratio, and tumor necrosis factor-α in various stages of chronic kidney disease",
"abstract": "<p>Chronic inflammation can have a considerable impact on the progression of glomerular and tubulointerstitial pathologies in chronic kidney disease (CKD). As an alternative to the early detection of inflammatory biomarkers that are most prominent in the progression of CKD, analyzing the relationship of monocyte-to-lymphocyte ratio (MLR), neutrophil-to-lymphocyte ratio (NLR), and tumor necrosis factor-α (TNF-α) in CKD is the goal of this study. This cross-sectional study involved 65 CKD subjects, consisting of 5 stages of CKD in compliance with KDIGO standards. Estimated glomerular filtration rate (eGFR) was calculated utilizing the CKD epidemiology collaboration (CKD-EPI) creatinine formulation. MLR and NLR were calculated from the differential count hematology analyzer. TNF-α was examined using the sandwich ELISA method. Random-spot urine was used for the UACR examination. There were significant differences in MLR and TNF-α at various stages of CKD, whereas NLR exhibited no significant difference at different stages of CKD. There was a significant association in MLR, NLR, and the stages of CKD. However, TNF-α was not associated with the stages of CKD. Also, MLR and NLR were not correlated with TNF-α in CKD. Furthermore, MLR, NLR, and TNF-α had no significant relationship with urine albumin-creatinine ratio (UACR). In conclusion, MLR is better for assessing CKD progression than NLR and TNF-α.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d29",
"views": 1139,
"downloads": 226,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/22/16/178-1700990889-Figure1.jpg",
"caption": "Figure 1. Schematic illustration of the study on the association between monocyte-to-lymphocyte ratio, neutrophil-to-lymphocyte ratio, and tumor necrosis factor-alpha in various stages of chronic kidney disease (CKD).",
"featured": false
}
],
"authors": [
{
"id": 1350,
"affiliation": [
{
"affiliation": "Department of Basic Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya 60132, Indonesia"
}
],
"first_name": "Desty Indah",
"family_name": "Sari",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 282
},
{
"id": 1351,
"affiliation": [
{
"affiliation": "Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya 60132, Indonesia"
}
],
"first_name": "Puspa",
"family_name": "Wardhani",
"email": "puspa-w-2@fk.unair.ac.id",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-2202-8090",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Puspa Wardhani, Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya 60132, Indonesia, Email: puspa-w-2@fk.unair.ac.id",
"article": 282
},
{
"id": 1352,
"affiliation": [
{
"affiliation": "Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya 60132, Indonesia"
}
],
"first_name": "Yessy",
"family_name": "Puspitasari",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 282
},
{
"id": 1353,
"affiliation": [
{
"affiliation": "Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya 60132, Indonesia"
},
{
"affiliation": "Universitas Airlangga Hospital, Surabaya 60115, Indonesia"
}
],
"first_name": "Satriyo Dwi",
"family_name": "Suryantoro",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 282
}
]
},
{
"id": 281,
"slug": "178-1704623969-characterization-of-spantetaspan-gene-of-spanescherichia-colispan-isolated-from-colibacillosis-affected-calves-in-rangpur-bangladesh",
"type": "original_article",
"manuscript_id": "178-1704623969",
"published": "2024-03-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/19/178-1704623969.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 336-345",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Jihad JH, Rahman MM, et al. Characterization of tetA gene of Escherichia coli isolated from colibacillosis affected calves in Rangpur, Bangladesh. J Adv Biotechnol Exp Ther. 2024; 7(2): 336-345.",
"title": "Characterization of <span>tetA</span> gene of <span>Escherichia coli</span> isolated from colibacillosis affected calves in Rangpur, Bangladesh",
"abstract": "<p>Antimicrobial resistance is a matter of threat in global public health nowadays as a great number of antibiotics have lost their potential. The current study aimed to characterize the <em>tet</em>A gene involved in tetracycline resistance of <em>Escherichia coli</em> in colibacillosis affected calves. To perform the study, a total of 69 fecal samples from colibacillosis suspected diarrheic calves were collected randomly from different areas of Rangpur division in Bangladesh. Standard laboratory protocols were followed for isolation and identification of <em>E. coli</em>. Then bacterial sensitivity was tested by culturing the bacteria in Mueller Hinton agar by following the protocol of Kirby-bauer disc diffusion method. Tetracycline resistant gene was specified by using <em>tet</em>A primer by searching <em>tet</em>A gene and the resistant gene was sequenced by sanger sequencing method. A phylogenetic tree of nucleotide sequences of <em>tet</em>A gene of <em>E. coli </em>was formulated using NCBI blast to characterize the gene. From 69 fecal samples, 54 were positive for <em>E. coli</em>. In antibiogram study, 100% isolates of <em>E. coli </em>were resistant to amoxicillin, ampicillin, cefoxitin, erythromycin and imipenem and the nature of the isolates of <em>E.coli</em> against other antibiotics were as follows: doxycycline 64.8%, co-trimethoprim 63%, tetracycline 59.3%, ceftriaxone 50%, gentamicin 16.7%, levofloxacin 14.8% respectively. This study noticed that about 56.25% samples (18 from 32) were resistant to tetracycline due to <em>tet</em>A gene and the gene (<em>tetA</em> from <em>E. coli</em>) was closely related to the corresponding gene of <em>Klebsiella pneumoniae</em>, <em>Shigella sonnei</em>, <em>Proteus mirabilis</em> and <em>Citrobacter braakii</em> strains besides <em>E. coli</em>. This study suggests determining the other genes that are specific for tetracycline resistance in <em>E. coli </em>in Rangpur for better understanding the nature of it.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d28",
"views": 1570,
"downloads": 164,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/27/02/178-1704623969-Figure1.jpg",
"caption": "Figure 1. Cultural characteristics of the isolates of E. coli are as follows: grayish white, circular, opaque colonies were appeared in nutrient agar media (A), rose pink colored colonies in MacConkey agar media (B) and characteristic green metallic sheen-colored colonies were observed in Eosin methylene blue agar media (C).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/27/02/178-1704623969-Figure2.jpg",
"caption": "Figure 2. After observing the cultural characteristics of E. coli, several biochemical tests were performed. The properties of E. coli seen in different biochemical tests are as follows: bubbles were formed by E. coli indicating oxidase positive (A), red colored ring was visualized in methyl red test (B), pink colored ring in Indole test (C), the isolates were motile and urease was negative in MIU media (D), slant and butt were acidic and huge amount of gas was formed in TSI media (E), no changes were observed in VP media (F) and in citrate utilization test, E. coli was also negative (G).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/27/02/178-1704623969-Figure3.jpg",
"caption": "Figure 3. Confirmation of E. coli by observing 232bp band through amplification of 16s rRNA gene by PCR. Lane L: 1kb DNA ladder; Lane 1-6: DNA samples of bacteria isolated from different calves.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/27/02/178-1704623969-Figure4.jpg",
"caption": "Figure 4. Identification of tetracycline resistant gene of E. coli by amplification of tetA gene through PCR indicating 577bp. Lane L: 1kb DNA ladder; Lane 1-6: DNA of bacteria isolated from different calves.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/27/02/178-1704623969-Figure5.jpg",
"caption": "Figure 5. Phylogenetic analysis of tetA gene based on the nucleotide sequences. The Neighbor-Joining method with maximum composite likelihood nucleotide substitution model was followed by using MEGA11 software. The isolates sequence in this study is tetA HSTU which have the same clade as the previous studies.",
"featured": false
}
],
"authors": [
{
"id": 1347,
"affiliation": [
{
"affiliation": "Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dianjpur-5200, Bangladesh"
}
],
"first_name": "Jakiul Hasan",
"family_name": "Jihad",
"email": "jihadhstu5200@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0009-0004-7497-2221",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Jakiul Hasan Jihad, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh. Email: jihadhstu5200@gmail.com",
"article": 281
},
{
"id": 1348,
"affiliation": [
{
"affiliation": "Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dianjpur-5200, Bangladesh"
}
],
"first_name": "Md Mostafizer",
"family_name": "Rahman",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 281
},
{
"id": 1349,
"affiliation": [
{
"affiliation": "Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dianjpur-5200, Bangladesh"
}
],
"first_name": "Nazmi Ara",
"family_name": "Rumi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 281
}
]
},
{
"id": 280,
"slug": "178-1703475929-study-on-assessment-of-reproductive-hormones-in-male-patients-with-type-1-and-2-diabetes-mellitus",
"type": "original_article",
"manuscript_id": "178-1703475929",
"published": "2024-03-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/34/178-1703475929.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 328-335",
"academic_editor": "Md Saidur Rahman, PhD; University of Missouri, USA",
"cite_info": "Hashim NA., Madhi R, et al. Study on assessment of reproductive hormones in male patients with type 1 and 2 diabetes mellitus. J Adv Biotechnol Exp Ther. 2024; 7(2): 328-335.",
"title": "Study on assessment of reproductive hormones in male patients with type 1 and 2 diabetes mellitus",
"abstract": "<p>The process and viability of fertility are affected by diabetes mellitus, which is also associated with hyperglycemia. Males who experience hypogonadism or anxiety may find it difficult to feel sexually exhilarated. The aim of the present study is to assess reproductive hormone levels in type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) male patients. This study included 79 healthy controls and 158 male patients with T1DM and T2DM between the ages of 20 and 49 years. Levels of reproductive hormones such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), and prolactin (PRL) were measured. The results showed notable variations in the levels of these hormones among patients of different ages. PRL levels were significantly elevated in T1DM compared to T2DM in the age groups of 20-29 and 30-39. FSH and LH levels in all age groups were greatly increased in T1DM compared to T2DM, but T hormone levels were dramatically reduced in T1DM patients compared to T2DM patients. Additionally, patients with a disease duration of 11–15 years demonstrated a significant decrease in T hormone compared to those with a disease duration of 1–5 years. Because of age-related variations in hormone levels between T1DM and T2DM as well as a decline in T hormone levels over the course of the disease, high levels of PRL hormone may therefore exacerbate the effects of diabetes, which in turn may influence the reproductive hormones.</p>",
"DOI": "10.5455/jabet.2024.d27",
"views": 1381,
"downloads": 162,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/01/178-1703475929-Figure1.jpg",
"caption": "Figure 1. Levels of FSH, LH, T, and PRL in T1DM male patients and controls according to age. Serum levels of (A) FSH, (B) LT, (C) T, (D) PRL for age group (20-29) year, (E) FSH, (F) LT, (G) T, (H) PRL for age group (30-39) year, and (I) FSH, (J) LT, (K) T, (L) PRL for age group (20-29) year in T1DM male patients. The blue box represents a healthy control group. The green box represents T1DM male patients. Data represent means ± SEM. # P <0.05 versus control.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/01/178-1703475929-Figure2.jpg",
"caption": "Figure 2. Levels of FSH, LH, T, and PRL in T2DM male patients and controls according to age. Serum levels of (A) FSH, (B) LT, (C) T, (D) PRL for age group (20-29) year, (E) FSH, (F) LT, (G) T, (H) PRL for age group (30-39) year, and (I) FSH, (J) LT, (K) T, (L) PRL for age group (20-29) year in T2DM male patients. The blue box represents a healthy control group. The green box represents T2DM male patients. Data represent means ± SEM. # P <0.05 versus control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/01/178-1703475929-Figure3.jpg",
"caption": "Figure 3. Levels of FSH, LH, T, and PRL in T1DM and T2DM male patients. Serum levels of (A) FSH, (B) LT, (C) T, (D) PRL for age group (20-29) year, (E) FSH, (F) LT, (G) T, (H) PRL for age group (30-39) year, and (I) FSH, (J) LT, (K) T, (L) PRL for age group (20-29) year in T1DM and T2DM male patients. The blue box represents T1DM male patients. The green box represents T2DM male patients. Data represent means ± SEM. # P <0.05 versus T1DM.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/01/178-1703475929-Figure4.jpg",
"caption": "Figure 4. FSH, LH, T, PRL levels in T1DM and T2DM male patients, regardless of age and based on the period of disease. Serum levels of (A) FSH, (B) LT, (C) T, and (D) PRL in T1DM and T2DM male patients and controls. The white box represents a healthy control group. The blue box represents T1DM male patients. The green box represents T2DM male patients. Data represent means ± SEM. N.S: no significant between groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/01/178-1703475929-Figure5.jpg",
"caption": "Figure 5. Levels of (A) FSH, (B) LT, (C) T, and (D) PRL in T1DM and T2DM male patients according to duration of disease. The white box represents a period (1-5) year. The blue box represents period (6-10) year. The green box represents a period (11-15) year. Data represent means ± SEM. # P <0.05 versus (1-5) year.",
"featured": false
}
],
"authors": [
{
"id": 1342,
"affiliation": [
{
"affiliation": "Southern Technical University, Amara Technical institute, 62001 Maysan, Iraq"
}
],
"first_name": "Nidhal A.",
"family_name": "Hashim",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 280
},
{
"id": 1343,
"affiliation": [
{
"affiliation": "Department of Biology, College of Science, University of Misan, 62001 Maysan, Iraq"
}
],
"first_name": "Raed",
"family_name": "Madhi",
"email": "raedsaddam@uomisan.edu.iq",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-7034-646X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Raed Madhi, PhD ; Department of Biology, College of Science, University of Misan, Maysan, Iraq . Email: raedsaddam@uomisan.edu.iq",
"article": 280
},
{
"id": 1344,
"affiliation": [
{
"affiliation": "Department of Biology, College of Science, University of Misan, 62001 Maysan, Iraq"
}
],
"first_name": "Zainab A.J. R.",
"family_name": "Al-Ali",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 280
},
{
"id": 1345,
"affiliation": [
{
"affiliation": "Southern Technical University, Amara Technical institute, 62001 Maysan, Iraq"
}
],
"first_name": "Ali A.",
"family_name": "Syhood",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 280
},
{
"id": 1346,
"affiliation": [
{
"affiliation": "Al-Sadr General Hospital, Misan Health Department, 62001 Maysan, Iraq"
}
],
"first_name": "Hayder",
"family_name": "Khion",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 280
}
]
},
{
"id": 279,
"slug": "178-1705074414-coffee-arabica-derived-copper-nanoparticles-a-potent-larvicidal-agent-against-aedes-aegypti-mosquitoes",
"type": "original_article",
"manuscript_id": "178-1705074414",
"published": "2024-02-29",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/14/178-1705074414.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 314-327",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Abdulwahab Y, Ahmad A, et al. Coffee arabica-derived copper nanoparticles: A potent larvicidal agent against Aedes aegypti mosquitoes. J Adv Biotechnol Exp Ther. 2024; 7(2): 314-327.",
"title": "<span>Coffee arabica</span>-derived copper nanoparticles: A potent larvicidal agent against <span>Aedes aegypti</span> mosquitoes",
"abstract": "<p>Mosquito-borne diseases, transmitted by <em>Aedes aegypti </em>mosquitoes, are a major public health concern. This study aimed to examine the larvicidal activity of <em>Coffee arabica</em>-mediated copper nanoparticles (CuNPs) against <em>Aedes aegypti </em>mosquito larvae. CuNPs were synthesized from <em>Coffee arabica</em> extract and characterized using advanced techniques such as UV spectroscopy, FTIR, and X-ray diffraction. The extract contained bioactive compounds with antioxidant properties. Green coffee had higher total phenolic content and total flavonoid capacity than roasted coffee. Green coffee extract exhibited stronger antioxidant activity and lower inhibitory concentration (IC<sub>50</sub>) values compared to roasted coffee extract. The larvicidal effects of green and roasted <em>Coffee arabica</em> extracts were assessed. Green <em>Coffee arabica</em> had lethal concentration (LC<sub>50</sub>) and LC<sub>90</sub> of 124.5 ppm and 456.5 ppm, respectively, while roasted <em>Coffee arabica</em> had LC<sub>50</sub> and LC<sub>90</sub> of 73.5 ppm and 371.5 ppm, respectively. CuNPs at concentrations of 2 to 100 ppm resulted in LC<sub>50</sub> and LC<sub>90</sub> of 5.796 ppm and 36.595 ppm, respectively. The synthesized CuNPs demonstrated concentration-dependent mortality, with lower LC<sub>50</sub> values compared to coffee extracts alone. Histological analysis showed tissue degradation in treated larvae, particularly in the head region. CuNPs had a detrimental effect on <em>Artemia salina</em> larvae, with higher concentrations leading to increased mortality. The LC<sub>50</sub> and LC<sub>90</sub> values for <em>Artemia salina</em> were determined as 344.3 ppm and 1073.2 ppm, respectively. Overall, <em>Coffee arabica</em>-mediated CuNPs displayed potent larvicidal activity against <em>Aedes aegypti </em>mosquitoes, indicating their potential use in eco-friendly mosquito control strategies.</p>",
"DOI": "10.5455/jabet.2024.d26",
"views": 2323,
"downloads": 190,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/01/178-1705074414-Figure1.jpg",
"caption": "Figures 1. A comparison of green and roasted coffee samples. Total Phenol average as GAE in mg/g and total flavonoid average as QE in mg/g, and average chlorogenic acid and caffeine contents in mg/g (data for chlorogenic acid and caffeine content was taken from Sualeh et al., (2020). Bars labelled 'a, b, c, d, e, f, g' are statistically significant with a p-value < 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/01/178-1705074414-Figure2.jpg",
"caption": "Figure 2. The average antioxidant activity (% DPPH) for each green and roasted Coffee arabica, Bars labeled a, b, c, d, e, f are statistically significant with a p-value < 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/01/178-1705074414-Figure3.jpg",
"caption": "Figure 3. Microscope images of Aedes aegypti larvae show distinct body parts (H: head, TH: thorax, AB: abdomen, S: respiratory siphon, AP: anal papilla). Control group (A-C), CuNPs treatment after 24-h exposure (D-F). Red arrows are a sign of localized body contraction, destruction and pigmentation.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/01/178-1705074414-Figure4.jpg",
"caption": "Figure 4. Histological sections of Aedes aegypti. Panels (A) and (B) depict control larvae, while panels (C) and (D) show larvae exposed to Coffee arabica extract-mediated CuNPs. The red arrows indicate areas of damage, including the epithelium layer, muscle, and mid-gut content.",
"featured": false
}
],
"authors": [
{
"id": 1337,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar-90245, Indonesia"
},
{
"affiliation": "Department of Biology, Faculty of applied Sciences, Taiz University, Taiz, Yemen"
}
],
"first_name": "Yousef",
"family_name": "Abdulwahab",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0009-4019-5141",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 279
},
{
"id": 1338,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar-90245, Indonesia"
}
],
"first_name": "Ahyar",
"family_name": "Ahmad",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-0913-4721",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ahyar Ahmad; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar-90245, Indonesia. Email: ahyarahmad@gmail.com",
"article": 279
},
{
"id": 1339,
"affiliation": [
{
"affiliation": "Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makassar-90245, Indonesia"
}
],
"first_name": "Isra",
"family_name": "Wahid",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-6642-1401",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 279
},
{
"id": 1340,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar-90245, Indonesia"
}
],
"first_name": "Paulina",
"family_name": "Taba",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0001-7327-5505",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 279
}
]
},
{
"id": 278,
"slug": "178-1705015163-detection-of-multi-antibiotic-resistant-vibrio-parahaemolyticus-isolated-from-fresh-produce-in-dhaka-bangladesh",
"type": "original_article",
"manuscript_id": "178-1705015163",
"published": "2024-02-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/24/178-1705015163.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 303-313",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Akter S, Zuhura FT, et al. Detection of multi-antibiotic resistant Vibrio parahaemolyticus isolated from fresh produce in Dhaka, Bangladesh. J Adv Biotechnol Exp Ther. 2024; 7(2): 303-313.",
"title": "Detection of multi-antibiotic resistant <span>Vibrio parahaemolyticus</span> isolated from fresh produce in Dhaka, Bangladesh",
"abstract": "<p>The demand for fresh produce is rising daily as it's an excellent way to maintain a healthy lifestyle. Thus, the current study aimed to employ culture-based and molecular techniques for isolating and identifying the pathogenic <em>Vibrio parahaemolyticus</em> from fresh produce. A total of 112 fresh produces of 14 different types were purchased from venders. The colonial appearance on thiosulfate citrate bile salt sucrose agar and CHROMagar culture plates revealed the prevalence of <em>Vibrio </em>spp. and <em>V. parahaemolyticus</em> in all examined samples, which were 45/112 (40.2%) and 40/112 (35.7%), with the highest load of up to 2.0x10<sup>5</sup>cfu/g and 6.4x10<sup>3</sup>cfu/g, respectively. Sixty isolates in all were chosen for further molecular characterization. Multiplex PCR results exhibited that all tested isolates were positive for <em>tlh</em> gene and six for <em>tdh</em> gene but none of isolate for <em>trh</em> gene. According to obtained data on antimicrobial susceptibility, the major portion of tested strains was found to be resistant to ampicillin (90%), tetracycline (80%), and streptomycin (70%). The range of the multiple antibiotic index was 0-0.33. This study finding revealed that the existence of potential pathogenic <em>V. parahaemolyticus</em> in tested fresh produce samples constitutes a risk to public health and consumer safety, thus urging continued surveillance.</p>",
"DOI": "10.5455/jabet.2024.d25",
"views": 1293,
"downloads": 174,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/24/178-1705015163-Figure1.jpg",
"caption": "Figure 1. Expected colonies of V. parahaemolyticus on A) TCBS agar plate appearing green color colonies B) CHROMAgar plate appearing mauve color colonies.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/24/178-1705015163-Figure2.jpg",
"caption": "Figure 2. Multiplex PCR of V. parahaemolyticus acquired from fresh produce samples. Marker (M): 100 bp DNA ladder; + ve control: V. parahaemolyticus ATCC BAA-238; Lane 1-11: tlh (450 bp) gene amplification visualize band; Lane 1, 2, 5, 6, 7, 8: tdh (269 bp) gene amplification visualize band.",
"featured": true
}
],
"authors": [
{
"id": 1331,
"affiliation": [
{
"affiliation": "Department of Botany, Jagannath University, Bangladesh"
}
],
"first_name": "Sumi",
"family_name": "Akter",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0009-5909-6821",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sumi Akter, PhD; Department of Botany, Jagannath University, Dhaka-1100, \r\nBangladesh. Email: sumi.plant@gmail.com",
"article": 278
},
{
"id": 1332,
"affiliation": [
{
"affiliation": "Department of Botany, Jagannath University, Bangladesh"
}
],
"first_name": "Fatema-Tuz",
"family_name": "Zuhura",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 278
},
{
"id": 1333,
"affiliation": [
{
"affiliation": "Department of Zoology, University of Dhaka, Bangladesh"
}
],
"first_name": "Zannatul",
"family_name": "Ferdous",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 278
},
{
"id": 1334,
"affiliation": [
{
"affiliation": "Department of Botany, Jagannath University, Bangladesh"
}
],
"first_name": "Md. Nazmul",
"family_name": "Hossain",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 278
},
{
"id": 1335,
"affiliation": [
{
"affiliation": "Department of Botany, Jagannath University, Bangladesh"
}
],
"first_name": "Md. Tanvir",
"family_name": "Ahsan",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 278
},
{
"id": 1336,
"affiliation": [
{
"affiliation": "Directorate of Secondary and Higher Education Division, Ministry of Education, Dhaka, Bangladesh"
}
],
"first_name": "Md. Abdus",
"family_name": "Salam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 278
}
]
},
{
"id": 277,
"slug": "178-1702904050-evaluation-of-the-effectiveness-of-low-level-laser-therapy-on-proliferation-of-fibroblasts-isolated-from-chronic-wounds-in-human-in-vitro",
"type": "original_article",
"manuscript_id": "178-1702904050",
"published": "2024-02-21",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/18/178-1702904050.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 290-302",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Phan MH, Nguyen-Thi BP, et al. Evaluation of the effectiveness of low-level laser therapy on proliferation of fibroblasts isolated from chronic wounds in human in vitro. J Adv Biotechnol Exp Ther. 2024; 7(2): 290-302.",
"title": "Evaluation of the effectiveness of low-level laser therapy on proliferation of fibroblasts isolated from chronic wounds in human in vitro",
"abstract": "<p>Chronic wounds pose significant challenges in healthcare due to impaired healing mechanisms. Fibroblast cells play a crucial role in wound healing by orchestrating proliferation and migration. This study aimed to assess fibroblast cells derived from chronic wounds and explore the impact of low-level laser therapy (LLLT) on their growth and migration. Dermal samples from chronic pressure ulcers and diabetic ulcers were obtained from 20 patients at three sites. Fibroblasts from wound base, margins, and adjacent healing skin were isolated and characterized. Proliferation and migration capabilities of these cells were evaluated. LLLT was applied at various energy levels (2.5, 3, 3.5, 4, and 5 J/cm<sup>2</sup>) to assess its effect on cell count. Fibroblasts from chronic wounds exhibited slower proliferation and migration rates compared to normal dermal fibroblasts. Notably, LLLT intervention at different energy levels led to a significant increase in cell count, with the most pronounced effect observed at 3 J/cm<sup>2</sup>. LLLT at an energy level of 3 J/cm<sup>2</sup> demonstrated a notable enhancement in fibroblast migration. These findings underscore the potential of LLLT as a therapeutic approach for chronic wounds, offering insights into its efficacy in augmenting fibroblast functions crucial for wound healing.</p>",
"DOI": "10.5455/jabet.2024.d24",
"views": 1651,
"downloads": 222,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/12/22/178-1702904050-Figure1.jpg",
"caption": "Figure 1. Growth time of fibroblast cell generations in vitro. The figure represents the average growth time (in days) of fibroblast cell generations cultured in vitro for each sample group at a different site. A) The initiation phase of cell culture; B) The growth phase of the first generation (G1); C) The growth phase of the second generation (G2); D) The growth phase of the third generation (G3).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/22/178-1702904050-Figure2.jpg",
"caption": "Figure 2. The percentage of fibroblast samples cultured over generations for different patient groups and sites. Figure 2 depicts the success rates of fibroblast samples cultured across three generations (G1, G2, G3) at distinct sites (Site 1, Site 2, and Site 3) for pressure and diabetic ulcer groups. Success rates declined over generations, notably at the wound base site (Site 1), suggesting reduced proliferation or availability of viable cells. The pressure ulcer group maintained higher success rates at Sites 2 and 3. Further research into factors impacting multi-generational fibroblast growth and viability is warranted, emphasizing differences between wound locations.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/22/178-1702904050-Figure3.jpg",
"caption": "Figure 3. Proliferation of fibroblast cells from Pressure Ulcer Wounds from Site 2, Site 3, and control samples on Day 1 (Magnification: 200) and Day 5 (Magnification: 200). These cells were derived from the third generation of fibroblasts cultured from Pressure Ulcer Wound samples.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/30/22/178-1702904050-Figure4.jpg",
"caption": "Figure 4. Average cell count (105) of cell proliferation from Pressure Ulcer Wounds in samples from site 2, site 3, and control derived from pressure ulcer wound after 5 days of cultivation in the 3rd generation. (*) denotes statistical significance compared to the control group (p < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/22/178-1702904050-Figure5.jpg",
"caption": "Figure 5. The Impact of LLLT on fibroblast cell from Pressure Ulcer Wounds growth at different energy levels. (*) denotes statistical significance compared to the control group (p < 0.05).",
"featured": false
}
],
"authors": [
{
"id": 1324,
"affiliation": [
{
"affiliation": "Ho Chi Minh city Hospital of Rehabilitation and Occupational Disease, 313 Au Duong Lan, Ward 2, District 8, Ho Chi Minh 71100, Vietnam"
}
],
"first_name": "Minh-Hoang",
"family_name": "Phan",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0008-9787-9141",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 277
},
{
"id": 1325,
"affiliation": [
{
"affiliation": "Vietnam National Institute of Burns, 263 Phung Hung Street, Ha Dong District, Ha Noi 11000, Vietnam"
}
],
"first_name": "Bich-Phuong",
"family_name": "Nguyen-Thi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 277
},
{
"id": 1326,
"affiliation": [
{
"affiliation": "Vietnam National Institute of Burns, 263 Phung Hung Street, Ha Dong District, Ha Noi 11000, Vietnam"
}
],
"first_name": "Van-Han",
"family_name": "Dinh",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 277
},
{
"id": 1327,
"affiliation": [
{
"affiliation": "Vietnam National Institute of Burns, 263 Phung Hung Street, Ha Dong District, Ha Noi 11000, Vietnam"
}
],
"first_name": "Thi-Huong",
"family_name": "Nguyen",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 277
},
{
"id": 1328,
"affiliation": [
{
"affiliation": "Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, An Khanh Ward, Ninh Kieu District, Can Tho 94000, Vietnam"
}
],
"first_name": "Ngoc-Nga",
"family_name": "Pham-Thi",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0002-9321-132X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 277
},
{
"id": 1329,
"affiliation": [
{
"affiliation": "Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, An Khanh Ward, Ninh Kieu District, Can Tho 94000, Vietnam"
}
],
"first_name": "Hong-Ha",
"family_name": "Nguyen",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0009-0008-4441-8173",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Hong-Ha Nguyen, PhD; Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, An Khanh Ward, Ninh Kieu District, Can Tho 94000, Vietnam. \r\nEmail: nhha82@ctump.edu.vn",
"article": 277
},
{
"id": 1330,
"affiliation": [
{
"affiliation": "Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, An Khanh Ward, Ninh Kieu District, Can Tho 94000, Vietnam"
}
],
"first_name": "Trung-Son",
"family_name": "Le",
"email": null,
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-3743-2902",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 277
}
]
},
{
"id": 276,
"slug": "178-1701606596-genome-wide-identification-and-characterization-of-interleukin-18-gene-family-in-rainbow-trout-oncorhynchus-mykiss",
"type": "original_article",
"manuscript_id": "178-1701606596",
"published": "2024-02-18",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/32/178-1701606596.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 275-289",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Bari SM, Hossen MI, et al. Genome-wide identification and characterization of interleukin-18 gene family in rainbow trout (Oncorhynchus mykiss). J Adv Biotechnol Exp Ther. 2024; 7(2): 275-289.",
"title": "Genome-wide identification and characterization of interleukin-18 gene family in rainbow trout (<span>Oncorhynchus mykiss</span>)",
"abstract": "<p>Rainbow trout (<em>Oncorhynchus mykiss</em>) are cold freshwater fish species belonging to the Salmonidae family, and they hold significant economic importance in the global aquaculture industry. The interleukin-18 (IL-18) gene family plays a crucial role in the immune response of rainbow trout when combating viral infections. A wide range of computational approaches were conducted to investigate the functions, phylogeny, and expressions of IL-18 family genes. The study found that there were nine IL-18 genes present on eight chromosomes of rainbow trout, and their encoded proteins were predicted to be distributed in the cytoplasm and nucleus. Genetic structures, motif and conserved domains analysis revealed that IL-18 genes in the same group have similar exons, motifs, and conserved domains. The phylogenetic study demonstrates the divergence of 38 IL-18 genes from three fish species into three distinct subgroups across time. The synteny analysis revealed the evolutionary mechanisms, such as gene duplications and mutations. In addition, the research investigated the expression of IL-18 genes in the liver tissues of rainbow trout following infection with infectious hematopoietic necrosis virus. These findings provide a comprehensive understanding of the role of IL-18 genes in <em>O. mykiss</em> in response to pathogenic infection.</p>",
"DOI": "10.5455/jabet.2024.d23",
"views": 1333,
"downloads": 130,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/19/178-1701606596-Figure1.jpg",
"caption": "Figure 1. The subcellular localization analysis predicted the primary presence of OmIL18 proteins in the nucleus and cytoplasm, with the majority of proteins exhibiting a red color indicative of their localization in these cellular compartments.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/19/178-1701606596-Figure2.jpeg",
"caption": "Figure 2. Comparative phylogenetic relationships (using MEGA 11 and iTOL) among the IL18 genes of O. mykiss, Carassius auratus, and Salmo salar. IL-18 genes were categorized into three subgroups. Clade 1 represents the crimson color, clade 2 shows a light green color and clade 3 represents aqua color.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/19/178-1701606596-Figure3.png",
"caption": "Figure 3. Motif and domain prediction of OmIL-18 proteins. (A) Distribution of conserved motifs in OmIL-18 proteins, motifs are indicated by 10 different color boxes; the length of each motif is shown proportionally. (B) IL-1 superfamily domain in different OmIL-18 proteins. The scale bar is shown at the bottom.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/19/178-1701606596-Figure4.jpeg",
"caption": "Figure 4. The intron-exon structures were illustrated using green and black shapes, representing exons within the genes.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/19/178-1701606596-Figure5.jpg",
"caption": "Figure 5. The chromosomal distribution of the OmIL18 gene family in rainbow trout is depicted in the figure, where green bars represent individual chromosomes of the fish, and black lines signify functional relationships or co-regulation among OmIL18 genes.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/19/178-1701606596-Figure6.jpg",
"caption": "Figure 6. Visualization of the distribution of OmIL18 genes across chromosomes in rainbow trout, with lines connecting genes on different chromosomes indicating potential gene duplications.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/19/178-1701606596-Figure7.png",
"caption": "Figure 7. Gene ontology (GO) analysis of the biological process (GOBP), cellular component (GOCC), molecular process (GOMP).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/19/178-1701606596-Figure8.jpg",
"caption": "Figure 8. Illustration of the expression pattern of different OmIL18 genes in the liver of rainbow trout in response to infectious hematopoietic necrosis virus (IHNV).",
"featured": false
}
],
"authors": [
{
"id": 1320,
"affiliation": [
{
"affiliation": "Department of Aquatic Animal Health Management, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh"
}
],
"first_name": "Sayed Mashequl",
"family_name": "Bari",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-0074-9436",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sayed Mashequl Bari; Department of Aquatic Animal Health, Management, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh. Email: bari.aahm@sau.edu.bd",
"article": 276
},
{
"id": 1321,
"affiliation": [
{
"affiliation": "State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China"
}
],
"first_name": "Md Isrfil",
"family_name": "Hossen",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0009-0001-2189-1039",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 276
},
{
"id": 1322,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh"
}
],
"first_name": "Md Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0009-0000-9048-5213",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 276
},
{
"id": 1323,
"affiliation": [
{
"affiliation": "State Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Linfen, China"
}
],
"first_name": "Md Mostofa Uddin",
"family_name": "Helal",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-7033-3578",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 276
}
]
},
{
"id": 275,
"slug": "178-1705117510-epidemiological-and-hematological-aspects-of-idiopathic-diarrhea-in-dairy-cattle-at-sirajganj-district-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1705117510",
"published": "2024-02-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/41/178-1705117510.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(2): 266-274",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Hasan SMN, Islam MR, et al. Epidemiological and hematological aspects of idiopathic diarrhea in dairy cattle at Sirajganj district of Bangladesh. J Adv Biotechnol Exp Ther. 2024; 7(2): 266-274.",
"title": "Epidemiological and hematological aspects of idiopathic diarrhea in dairy cattle at Sirajganj district of Bangladesh",
"abstract": "<p>Cattle diarrhea poses a significant risk to the global livestock sector, leading to considerable economic setbacks and impacting the well-being of animals. This research aimed to assess the clinical and hematological changes associated with idiopathic diarrhea in cattle in the Baghabari area of the Sirajganj District of Bangladesh. The study involved surveying dairy cattle to record their case history, followed by the collection and measurement of hematological indices in those animals suffering from idiopathic diarrhea. The survey identified 39 diarrheic crossbred cattle (2 males and 37 females), ranging in age from 1 month to 11 years. The findings revealed a higher prevalence of diarrheal cases in cows (94.87%) compared to oxen (5.13%), particularly among pregnant cows (64.86%). The most common historical observations included repeat breeding (81.08%), abortion (78.38%), decreased milk production (43.24%), fever (15.64%), and congenital defects (17.95%) in diarrheic cattle. Hematological analyses indicated a significant increase in lymphocytes and immunoglobulin levels, while eosinophil, basophil, and red blood cell distribution width were notably lower in diarrheic cattle compared to healthy ones. However, other hematological parameters remained unaffected. In summary, the study suggests that bovine diarrhea may influence hematological profiles and body physiology, with the extent depending on the disease’s etiology and complexity. <strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d22",
"views": 1247,
"downloads": 218,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/08/178-1705117510-Figure1.jpg",
"caption": "Figure 1. Study area map, created by ArcMap 10.7 (ESRI, USA) based on Geographical Information System (GIS).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/08/178-1705117510-Figure2.jpg",
"caption": "Figure 2. Dynamics of total erythrocyte count (TEC), hemoglobin (Hb), total leukocyte count (TLC), and platelet (PLT) in blood collected from healthy and diarrheic cattle. Data were analyzed by paired sample t-test and presented as mean ± SEM.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/08/178-1705117510-Figure3.jpg",
"caption": "Figure 3. Dynamics of neutrophil (NEUT), lymphocyte (LYM), monocyte (MONO), eosinophil (EO), and basophil (BASO) in blood collected from healthy and diarrheic cattle. Data were analyzed by paired sample t-test and presented as mean ± SEM.",
"featured": false
}
],
"authors": [
{
"id": 1310,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "SM Nazmul",
"family_name": "Hasan",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 275
},
{
"id": 1311,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md. Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 275
},
{
"id": 1312,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Anandha",
"family_name": "Mozumder",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 275
},
{
"id": 1313,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md Ashek",
"family_name": "Ullah",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 275
},
{
"id": 1314,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Moslema Jahan",
"family_name": "Mou",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 275
},
{
"id": 1315,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Marjana",
"family_name": "Akter",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 275
},
{
"id": 1316,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Nurejunnati",
"family_name": "Jeba",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 275
},
{
"id": 1317,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md. Eftakhar Jahan",
"family_name": "Bhuiyan",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 275
},
{
"id": 1318,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Sharmin",
"family_name": "Akter",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 275
},
{
"id": 1319,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md. Golzar",
"family_name": "Hossain",
"email": null,
"author_order": 10,
"ORCID": "https://orcid.org/0000-0002-1487-5444",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Golzar Hossain, PhD; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh, \r\nEmail: mghossain@bau.edu.bd",
"article": 275
}
]
}
]
},
{
"id": 21,
"title": "Vol7 Issue1",
"cover": "https://jabet.bsmiab.org/media/issue_cover/Jabet_Coverpage_uTUksJ5.jpg",
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2024",
"slug": "2024-vol7-issue1",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 274,
"slug": "178-1701550511-effect-of-spangynura-procumbensspan-leaf-extract-with-biological-nanoparticles-on-streptozotocin-induced-hyperglycemia-in-a-rat-model",
"type": "original_article",
"manuscript_id": "178-1701550511",
"published": "2024-01-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/24/178-1701550511.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 255-265",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Badsha SA, Rakibul Islam R, et al. Effect of Gynura procumbens leaf extract with biological nanoparticles on streptozotocin-induced hyperglycemia in a rat model. J Adv Biotechnol Exp Ther. 2024; 7(1): 255-265.",
"title": "Effect of <span>Gynura procumbens</span> leaf extract with biological nanoparticles on streptozotocin-induced hyperglycemia in a rat model",
"abstract": "<p>Herbal medicines, a popular choice for treatments, are effective against diabetes when used with nanoparticles. Thus, this study investigates the effects of <em>Gynura procumbens</em> ethanolic extract with chitosan nanoparticles on streptozotocin-induced hyperglycemia in a rat model. Twenty-eight days old 16 male rats were randomly divided into 4 groups such as T0 = control (normal), T1 = streptozotocin-induced diabetes, T2 = streptozotocin-induced diabetes treated with ethanolic extract of <em>Gynura Procumbens</em> (150 mg/kg body weight), and T3= streptozotocin induced diabetes treated with ethanolic extract of <em>Gynura Procumbens</em> (150 mg/kg) and chitosan nanoparticles (25 mg/kg). Blood glucose levels were significantly higher in the diabetic group T1 (26.27±1.76) compared to treatment T2 (18.32±.715) and T3 (15.45±1.33) groups. The body weight of T1 (115.75±0.08c) significantly decreased compared to control T0 (216.25±4.27) on the 21st day. Interestingly, there was significant (p<0.01) weight gain in T2 and T3 than diabetic group T1. Also, insulin level peaked in T3 (0.41 µU/ml) statistically significant at (p<0.01), while it decreased in diabetic group T1 (0.41 µU/ml) compared to control T0 (0.34 µU/ml). Alanine transaminase (ALT) and aspartate aminotransferase (AST) were normal in control T0 (181.50 U/L and 139.00 U/L), while diabetic group T1 showed an increased level (463.75 U/L and 322.00 U/L). Interestingly, treatment of the plant extracts and nanoparticles (T3) effectively reduced ALT and ASL levels within the 21 days of treatment. In conclusion, <em>Gynura procumbens</em> ethanolic extract and chitosan nanoparticles can be used as an effective anti-diabetic treatment.</p>",
"DOI": "10.5455/jabet.2024.d21",
"views": 1498,
"downloads": 204,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/35/24/178-1701550511-Figure1.jpg",
"caption": "Figure 1. Histopathological observation of liver. A) Group T0= control normal rats, B) T1= streptozotocin-induced diabetic rats, C) T2= streptozotocin and Gynura Procumbens-treated rats and D) T3= streptozotocin-induced diabetic rat treated with Gynura Procumbens and chitosan nanoparticles. In the case of Group B, C, and D, increasement of the interlobular space in rat liver was observed by microscopic examination (10x), Scale bar=1 inch. Stained with hematoxylin & eosin: H & E. Arrow indicates the increasement of the interlobular space in rat liver.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/35/24/178-1701550511-Figure2.jpg",
"caption": "Figure 2. Histopathological observation of kidney. A) Group T0= control normal rats, B) T1= streptozotocin-induced diabetic rats, C) T2= streptozotocin and Gynura Procumbens-treated rats and D) T3= streptozotocin-induced diabetic rat treated with Gynura Procumbens and chitosan nanoparticles. Section of kidney showed no hemorrhagic lesions, no inflammatory infiltration and no other distinct changes was observed by microscopic examination at 10x (Scale bar=1 inch) stained with hematoxylin & eosin: H & E.",
"featured": false
}
],
"authors": [
{
"id": 1303,
"affiliation": [
{
"affiliation": "Department of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Shahadat Alam",
"family_name": "Badsha",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 274
},
{
"id": 1304,
"affiliation": [
{
"affiliation": "Department of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Rakibul",
"family_name": "Islam",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-0809-1819",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 274
},
{
"id": 1305,
"affiliation": [
{
"affiliation": "Department of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Mst. Misrat Masuma",
"family_name": "Parvez",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0001-9617-5961",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 274
},
{
"id": 1306,
"affiliation": [
{
"affiliation": "Department of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Bazlar",
"family_name": "Rashid",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 274
},
{
"id": 1307,
"affiliation": [
{
"affiliation": "Department of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Mahmudul",
"family_name": "Hasan",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 274
},
{
"id": 1308,
"affiliation": [
{
"affiliation": "School of Veterinary Medicine, Texas Tech University, 7671 Evans Drive, Amarillo, TX 79106, USA"
}
],
"first_name": "Vishakha",
"family_name": "Kulkarni",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0009-0009-6683-3877",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 274
},
{
"id": 1309,
"affiliation": [
{
"affiliation": "Department of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
},
{
"affiliation": "School of Veterinary Medicine, Texas Tech University, 7671 Evans Drive, Amarillo, TX 79106, USA"
}
],
"first_name": "Sumon",
"family_name": "Sarkar",
"email": "sarkarsumon.setu@gmail.com",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-9008-1208",
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "Sumon Sarkar;\r\nDepartment of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.\r\nEmail: sarkarsumon.setu@gmail.com",
"article": 274
}
]
},
{
"id": 273,
"slug": "178-1699955352-in-vivo-antidiarrheal-effect-and-phytochemical-characterization-of-spanplectranthus-barbatusspan-andrews",
"type": "original_article",
"manuscript_id": "178-1699955352",
"published": "2024-01-21",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/23/178-1699955352.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 239-254",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Ajwang EC, Ngugi MP, et al. In vivo antidiarrheal effect and phytochemical characterization of Plectranthus barbatus Andrews. J Adv Biotechnol Exp Ther. 2024; 7(1): 239-254",
"title": "In vivo antidiarrheal effect and phytochemical characterization of <span>Plectranthus barbatus</span> Andrews",
"abstract": "<p>Diarrheal infections remain a public health problem. The mainstays of pharmacological therapy for infectious diarrhea include probiotics, antibacterials, and intestinal adsorbents. Nevertheless, these clinical treatments are not devoid of shortcomings including prohibitive costs and adverse effects. Medicinal plants including <em>Plectranthus barbatus </em>(<em>P. barbatus</em>)<em> </em>have folkloric remedies against diarrhea. However, there is paucity of knowledge to scientifically validate the efficacy of <em>P. barbatus</em> on diarrheal infections. The study was undertaken to ascertain antidiarrheal efficacy, bioactive composition, and toxicity profiles of <em>P. barbatus</em> extracts. Antidiarrheal activity and acute toxicity were carried out on Swiss albino mice. Quantitative phytocompound analysis was evaluated using liquid chromatography-mass spectrometry (LCMS). Results indicated that <em>P. barbatus</em> extract has antidiarrheal activity whereby it prolonged the start of diarrhea, causing a decline in the occurrence of wet feces and intestinal transit significantly. Additionally, the<em> </em>extract reduced the accumulation of intraluminal fluid resulting in decrease in distension, intestinal overload, and water content in the fecal drops. Also, acute toxicity assay on mice showed that <em>P. barbatus </em>extract was non-toxic at the dosage level of 2000mg/kgbw. In addition, LCMS analysis detected presence of phytocompounds associated with antidiarrheal effects including forskolin, gallic, kaempferol, and quercetin. Findings from the current study offer a scientific validation for the folkloric utilization of <em>P. barbatus </em>in the management of diarrhea.</p>",
"DOI": "10.5455/jabet.2024.d20",
"views": 1603,
"downloads": 174,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/21/178-1699955352-Figure1.jpg",
"caption": "Figure 1. Inhibitory effects of P. barbatus extract on Gastrointestinal Motility. Group II (Water + Castor oil) was untreated, Group III (loperamide + Castor oil) and the Groups IV-VI (P. barbatus leaf extract at different dosages (100-400mg/kgbw) + Castor oil). The inhibition of charcoal meal assessed, and the values of peristaltic index measured. Each group consisted of 6 mice. Bars with different lowercase letter vary significantly (p <0.05) using one-way ANOVA followed by Tukey’s multiple comparison (Graph pad prism 8).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/21/178-1699955352-Figure2.jpg",
"caption": "Figure 2. Enteropooling effect of P. barbatus aqueous extract on castor oil-induced diarrhea in mice. Group II (Water + Castor oil) was untreated, Group III (loperamide + Castor oil) and the Groups IV-VI (P. barbatus leaf extract at different dosages (100-400mg/kgbw) + Castor oil), the weight and volume of the intestinal contents measured. Each group consisted of 6 mice. Bars with different lowercase letter vary significantly (p <0.05) using one-way ANOVA followed by Tukey’s multiple comparison (Graph pad prism 8).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/21/178-1699955352-Figure3.jpg",
"caption": "Figure 3. In vivo anti-diarrheal index of P. barbatus extract. Group II (Water+ Castor oil) was untreated, Group III (loperamide + Castor oil) and the Groups IV-VI (P. barbatus leaf extract at different dosages (100-400mg/kgbw + Castor oil). Each group consisted of 6 mice. The values obtained from the three models were used to measure the antidiarrheal effects of P. barbatus aqueous extract. Bars with different lowercase letters vary significantly (p <0.05) using one-way ANOVA followed by Tukey’s multiple comparison (Graph pad prism 8).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/21/178-1699955352-Figure4.jpg",
"caption": "Figure 4. Acute toxicity effects of P. barbatus extract on the organ weight. Normal control group was untreated, and the treatment group treated with P. barbatus leaf extract at dose of 2000mg/kgbw. The behavioral, neurological and physical aspects assessed, and the organs harvested, observed and weighed. Each group consists of 6 replicates. Bars at the same organ are insignificant using independent t-test (p >0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/21/178-1699955352-Figure5.jpg",
"caption": "Figure 5. LC-MS chromatogram of aqueous extract of P. barbatus. X-axis presents retention time whereas Y-axis presents the percentage abundance. Retention times of the phytoconstituents are shown above peaks.",
"featured": true
}
],
"authors": [
{
"id": 1299,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya"
},
{
"affiliation": "School of Medicine, Uzima University, Kisumu, Kenya"
}
],
"first_name": "Emmah Clarice",
"family_name": "Ajwang",
"email": "clariceadede@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0009-0002-1026-9591",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Emmah Clarice Ajwang; Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya \r\nEmail: clariceadede@gmail.com",
"article": 273
},
{
"id": 1300,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Mathew Piero",
"family_name": "Ngugi",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-0226-9092",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 273
},
{
"id": 1301,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "George Isanda",
"family_name": "Omwenga",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-1877-4464",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 273
},
{
"id": 1302,
"affiliation": [
{
"affiliation": "The Aga Khan University, Medical College, East Africa, Nairobi, Kenya"
}
],
"first_name": "Carolyn Wanjira",
"family_name": "Muruthi",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0001-9952-2789",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 273
}
]
},
{
"id": 272,
"slug": "178-1700321148-antiviral-potential-of-terpenoids-against-major-viral-infections-recent-advances-challenges-and-opportunities",
"type": "review_article",
"manuscript_id": "178-1700321148",
"published": "2024-01-17",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/40/178-1700321148.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 221-238.",
"academic_editor": "Md. Golzar Hossain, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Orosco FL, Quimque MTJ. Antiviral potential of terpenoids against major viral infections: Recent advances, challenges, and opportunities. J Adv Biotechnol Exp Ther. 2024; 7(1): 221-238.",
"title": "Antiviral potential of terpenoids against major viral infections: Recent advances, challenges, and opportunities",
"abstract": "<p>Terpenoids are a diverse class of phytochemicals derived from isoprene units that have shown significant potential as antiviral agents. This review aimed to comprehensively explore the antiviral activities of terpenoids and their implications in the field of virology. Key findings from various studies have demonstrated the efficacy of terpenoids against a wide spectrum of viruses, encompassing both enveloped and non-enveloped viruses. Mechanistic insights into their antiviral action involve interference with viral entry, replication, and modulation of the host immune responses. Their multifaceted mechanisms of action make them attractive targets for combating viral infections and offer opportunities for synergy with existing antiviral drugs. Despite substantial progress in this field, several gaps must be addressed, including elucidation of specific molecular targets and signaling pathways of different terpenoids, investigations of its safety and potential side effects, and optimization of formulations and delivery methods for better bioavailability and pharmacokinetics. In conclusion, this comprehensive review provides a foundation for continued exploration and innovation in terpenoid-based antiviral research with the ultimate aim of improving global health outcomes in the face of viral threats.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d19",
"views": 2132,
"downloads": 183,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/18/178-1700321148-Figure1.jpg",
"caption": "Figure 1. Chemical structure of volatile terpenes. (A) Hemiterpenes, (B) Monoterpenes, and (C) Sesquiterpenes [8].",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/18/178-1700321148-Figure2.jpg",
"caption": "Figure 2. Chemical structure of semi- and non-volatile terpenes (A) Diterpenes, (B) Triterpenes, and (C) Tetraterpenes [8].",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/18/178-1700321148-Figure3.jpg",
"caption": "Figure 3. Virus replication and terpenoid targets. Several terpenoids were identified in this review displaying inhibitory activity against different viruses by inhibiting essential steps in virus infection cycle such as binding, fusion, reverse transcription, transcription, translation, budding, and assembly.",
"featured": true
}
],
"authors": [
{
"id": 1297,
"affiliation": [
{
"affiliation": "Industrial Technology Development Institute, Department of Science and Technology, Bicutan, Taguig City, 1634, Philippines"
},
{
"affiliation": "S&T Fellows Program, Department of Science and Technology, Bicutan, Taguig City, 1634, Philippines"
},
{
"affiliation": "Department of Biology, College of Arts and Sciences, University of the Philippines Manila, Metro Manila, 1000, Philippines"
}
],
"first_name": "Fredmoore L.",
"family_name": "Orosco",
"email": "orosco.fredmoore@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-8861-7923",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Fredmoore L. Orosco, PhD;\r\nIndustrial Technology Development Institute, Department of Science and Technology, Bicutan, Taguig City, 1634, Philippines. Email: orosco.fredmoore@gmail.com",
"article": 272
},
{
"id": 1298,
"affiliation": [
{
"affiliation": "Department of Chemistry, Mindanao State University-Iligan Institute of Technology, Tibanga, Iligan City, 9200, Philippines"
}
],
"first_name": "Mark Tristan J.",
"family_name": "Quimque",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 272
}
]
},
{
"id": 271,
"slug": "178-1703176411-therapeutic-potential-of-selective-medicinal-plants-and-their-phytoconstituents-in-respiratory-diseases-a-review-with-an-emphasis-on-covid-19",
"type": "review_article",
"manuscript_id": "178-1703176411",
"published": "2024-01-16",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/55/178-1703176411.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 205-220",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Jubayer MF, Malek MA, et al. Therapeutic potential of selective medicinal plants and their phytoconstituents in respiratory diseases: A review with an emphasis on COVID-19. J Adv Biotechnol Exp Ther. 2024; 7(1): 205-220.",
"title": "Therapeutic potential of selective medicinal plants and their phytoconstituents in respiratory diseases: A review with an emphasis on COVID-19",
"abstract": "<p>Respiratory diseases, spanning from mild illnesses like the common cold to more serious problems like pneumonia and COVID-19, pose significant challenges to global health and contribute significantly to morbidity and death. The emergence of COVID-19 has increased the need for efficient therapeutic approaches. This review article examines the potential of specific medicinal plants, namely Black Cumin, Licorice, and Echinacea, and their phytoconstituents in the management of respiratory diseases, with a specific emphasis on COVID-19. It synthesizes extant knowledge on the therapeutic efficacy of these plants, which have been used traditionally in Unani, Ayurvedic, and Chinese medicine to alleviate respiratory symptoms and boost immune functions. This review also discusses the immunomodulatory and antiviral properties of these three specific medicinal plants, as well as their function in the treatment of a number of life-threatening diseases. Incorporating insights from ethnomedicine and contemporary scientific research, this review highlights the urgency of exploring herbal medicines as complementary therapies for respiratory diseases and offers perspectives on incorporating these natural remedies into contemporary treatment paradigms, particularly in the perspective of combating COVID-19.</p>",
"DOI": "10.5455/jabet.2024.d18",
"views": 2503,
"downloads": 250,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/18/178-1703176411-Figure1.jpg",
"caption": "Figure 1. Black cumin and its active constituents for COVID-19 and respiratory diseases treatment options.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/18/178-1703176411-Figure2.jpg",
"caption": "Figure 2. Mechanisms involved in pharmacological effects of N. sativa on COVID-19. Exploring the impact of seed nutrients on COVID-19 management. A multiphase therapeutic is clearly seen on oxidative stress reduction, inflammation control, and enhancement of immune function.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/18/178-1703176411-Figure3.jpg",
"caption": "Figure 3. Licorice and its active constituents for COVID-19 and respiratory treatment options.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/18/178-1703176411-Figure4.jpg",
"caption": "Figure 4. Comprehensive pathways of glycyrrhizin's antiviral activity against SARS-COV-2: viral membrane alteration, immune response modulation via macrophage activation and nitric oxide production, inhibition of cellular signaling pathways, and blockage of ACE2 receptor mediated entry.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/18/178-1703176411-Figure5.jpg",
"caption": "Figure 5. Echinacea and its active constituents for COVID-19 and respiratory treatment options.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/18/178-1703176411-Figure6.jpg",
"caption": "Figure 6. An illustration of possible mechanisms and effects of Echinacea on inflammatory cytokine inhibition and CB2/PPAR activation in the context of COVID-19 and respiratory diseases. Cannabinoid receptor 2, CB2R; Nuclear factor erythroid 2-related factor 2, Nrf2; Angiotensin-converting enzyme 2, ACE2; Transmembrane protease, serine 2, TMPRSS; Toll-like receptor 4, TLR4; Nuclear factor kappa B, NF-κB; Peroxisome proliferators–activated receptor γ, PPARγ.",
"featured": true
}
],
"authors": [
{
"id": 1285,
"affiliation": [
{
"affiliation": "Department of Food Engineering & Technology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Fahad",
"family_name": "Jubayer",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-4914-7284",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1287,
"affiliation": [
{
"affiliation": "Directorate General of Health Services, Ministry of Health and Family Welfare, Bangladesh"
}
],
"first_name": "Md Abdul",
"family_name": "Malek",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1288,
"affiliation": [
{
"affiliation": "Department of Physiology, Eastern Medical College, Cumilla, Bangladesh"
}
],
"first_name": "Towhidul",
"family_name": "Iqram",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1289,
"affiliation": [
{
"affiliation": "Mymensingh Medical College and Hospital, Mymensingh, Bangladesh"
}
],
"first_name": "Mahmuda",
"family_name": "Begum",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1286,
"affiliation": [
{
"affiliation": "Department of Food Engineering & Technology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Shahidullah",
"family_name": "Kayshar",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1290,
"affiliation": [
{
"affiliation": "Department of Agricultural Science, Daffodil International University, Dhaka-1216, Bangladesh"
}
],
"first_name": "Md. Shoaib",
"family_name": "Arifin",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0003-0922-6228",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1291,
"affiliation": [
{
"affiliation": "FETP,B Fellow (US-CDC), Institute of Epidemiology, Disease Control and Research (IEDCR), Dhaka, Bangladesh"
}
],
"first_name": "Md. Tariqul Islam",
"family_name": "Limon",
"email": null,
"author_order": 7,
"ORCID": "https://orcid.org/0000-0003-4204-4004",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1292,
"affiliation": [
{
"affiliation": "Department of Chemical and Food Process Engineering, Rajshahi University of Engineering & Technology, Rajshahi, Bangladesh"
}
],
"first_name": "Pabitra Chandra",
"family_name": "Das",
"email": null,
"author_order": 8,
"ORCID": "https://orcid.org/0000-0002-5876-3954",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1293,
"affiliation": [
{
"affiliation": "Department of Food Technology and Rural Industries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Anisur Rahman",
"family_name": "Mazumder",
"email": null,
"author_order": 9,
"ORCID": "https://orcid.org/0000-0002-0964-5521",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1294,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary, Animal, and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Margia",
"family_name": "Akter",
"email": null,
"author_order": 10,
"ORCID": "https://orcid.org/0000-0003-4118-0784",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1295,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": null,
"author_order": 11,
"ORCID": "https://orcid.org/0000-0003-2911-3255",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 271
},
{
"id": 1296,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Department of Pathology, Faculty of Veterinary, Animal, and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Masudur",
"family_name": "Rahman",
"email": "rahmanmm.dpp@sau.ac.bd",
"author_order": 12,
"ORCID": "https://orcid.org/0000-0001-7095-9303",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Masudur Rahman, PhD;\r\nDepartment of Pathology, Faculty of Veterinary, Animal, and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh\r\nEmail: rahmanmm.dpp@sau.ac.bd",
"article": 271
}
]
},
{
"id": 269,
"slug": "178-1697021466-pomegranate-fruit-peel-extract-improves-cardiac-functions-via-suppressing-oxidative-stress-fibrosis-and-myocardial-infarction-in-long-evans-rats",
"type": "original_article",
"manuscript_id": "178-1697021466",
"published": "2024-01-15",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/08/178-1697021466.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 190-204",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Kabir F, Nahar K, et al. Pomegranate fruit peel extract improves cardiac functions via suppressing oxidative stress, fibrosis, and myocardial infarction in Long-Evans rats. J Adv Biotechnol Exp Ther. 2024; 7(1): 190-204.",
"title": "Pomegranate fruit peel extract improves cardiac functions via suppressing oxidative stress, fibrosis, and myocardial infarction in Long-Evans rats",
"abstract": "<p>The pomegranate fruit (locally known as Dalim) is a rich source of various bioactive metabolites. This study aimed to investigate the cardioprotective effect of polyphenol-rich pomegranate peel extract (PPE) in isoproterenol, a synthetic catecholamine-induced cardiac stress in rats. Twenty-four adult rats were taken to evaluate the effects. The selected rats were divided into four groups, such as control (Group I), Isoproterenol (ISO) administered (Group II), PPE with ISO treatment (Group III), and gallic acid (polyphenol) along with ISO administration (Group IV). As expected, ISO administration increased inflammatory cell infiltration, fibrosis, and lipid peroxidation. However, antioxidant enzyme activities were reduced in heart tissues of ISO-treated rats. Interestingly, PPE treatment effectively reduced ISO-induced various detrimental effects in heart tissues in rats. This protection was associated with several positive outcomes, including reduced inflammatory cell infiltration and fibrosis, reduction in free radicals by improving antioxidant enzymes activities, suppression of oxidative stress markers, and reduction in inflammatory marker. Besides, PPE treatment normalized the elevated levels of liver and cardiac injury markers. The cardioprotective effects shown by PPE may be due to the presence of polyphenolic compounds, as determined by HPLC analysis. Overall, these data suggest that polyphenol enriched PPE may protect cardiac tissues in ISO-administered rats by suppressing oxidative stress, fibrosis, and myocardial infarction as well as enhancing antioxidant enzymes.</p>",
"DOI": "10.5455/jabet.2024.d17",
"views": 1520,
"downloads": 146,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/23/15/178-1697021466-Figure1.jpg",
"caption": "Figure 1. HPLC data showed the presence of gallic acid and rutin hydrate in the PPE.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/23/15/178-1697021466-Figure2.jpg",
"caption": "Figure 2. Effects of PPE on (A) AST, (B) ALT and (C) ALP activity in plasma of ISO-administered rats. Data are expressed as mean±SEM, n=6. ALT = alanine aminotransferase; AST = aspartate aminotransferase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/23/15/178-1697021466-Figure3.jpg",
"caption": "Figure 3. Effect of PPE on heart specific parameter CK-MB in plasma of ISO-administered rats. Data are expressed as mean±SEM, n=6. Creatinine kinase-MB, CK-MB.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/23/15/178-1697021466-Figure4.jpg",
"caption": "Figure 4. Effect of PPE on MPO activity in heart (A) and kidney (B) of ISO-administered rats. Data are expressed as mean±SEM, n=6. Myeloperoxidase, MPO.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/23/15/178-1697021466-Figure5.jpg",
"caption": "Figure 5. Effect of PPE on SOD (A-C) and catalase (D-F) activity in plasma, heart, and kidney of ISO-administered rats, respectively. Data are expressed as mean±SEM, n=6. SOD = superoxide dismutase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/23/15/178-1697021466-Figure6.jpg",
"caption": "Figure 6. Effects of PPE on oxidative stress parameters such as NO (A-C), MDA (D-F), and APOP (G-I) in plasma, heart, and kidney of ISO-treated rats, respectively. Data are expressed as mean±SEM, n=6. NO= nitric oxide, MDA= malondialdehyde and APOP= advanced protein oxidation product.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/23/15/178-1697021466-Figure7.jpg",
"caption": "Figure 7. Effect of PPE on the oxidative inflammation and tissue damage (upper panel) as well as collagen deposition and fibrosis (lower panel) in the heart of ISO-administered rats. A, E- control; B, F- ISO; C, G- ISO+PPE and D, H- ISO+GA. ISO = isoproterenol; PPE = pomegranate fruit peel extract; GA = gallic acid.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/23/15/178-1697021466-Figure8.jpg",
"caption": "Figure 8. Schematic proposed cardioprotective mechanism of action of pomegranate peel extract in ISO administered rats. ISO administration increased the autooxidation of catecholamine and increased the superoxide production in the heart. The sources of reactive oxygen species (ROS) may be NADPH oxidase, mitochondria or myeloperoxidase (MPO). ROS mediated oxidative stress may increase the lipid peroxidation and lower the antioxidant enzyme function. Moreover, ROS leading inflammatory response may increase the tissues damage and inflammation. PPE polyphenol gallic acid may directly scavenge the ROS as well as restore the antioxidant enzyme function in the heart and showed a protective effect is ISO administered rats.",
"featured": false
}
],
"authors": [
{
"id": 1264,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Fariha",
"family_name": "Kabir",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 269
},
{
"id": 1265,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Kamrun",
"family_name": "Nahar",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 269
},
{
"id": 1271,
"affiliation": [
{
"affiliation": "School of Pharmacy and Public Health, Independent University, Dhaka, Bangladesh"
}
],
"first_name": "Samia",
"family_name": "Hussain",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 269
},
{
"id": 1266,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Tahmina",
"family_name": "Yasmin",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 269
},
{
"id": 1272,
"affiliation": [
{
"affiliation": "School of Pharmacy and Public Health, Independent University, Dhaka, Bangladesh"
}
],
"first_name": "Nasrin",
"family_name": "Akhter",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 269
},
{
"id": 1267,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "M. Nurul",
"family_name": "Islam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 269
},
{
"id": 1268,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Md Mizanur",
"family_name": "Rahman",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 269
},
{
"id": 1273,
"affiliation": [
{
"affiliation": "Chemical from Indigenous Sources Section, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh"
}
],
"first_name": "Hemayet",
"family_name": "Hossain",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 269
},
{
"id": 1274,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Md. Areeful",
"family_name": "Haque",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 269
},
{
"id": 1269,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Md Ashraful",
"family_name": "Alam",
"email": "sonaliagun@yahoo.com",
"author_order": 10,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Ashraful Alam, Department of Pharmaceutical Sciences, North South University, Bangladesh. Email: sonaliagun@yahoo.com",
"article": 269
},
{
"id": 1270,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Nusrat",
"family_name": "Subhan",
"email": "nusrat.subhan@northsouth.edu",
"author_order": 11,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nusrat Subhan, Department of Pharmaceutical Sciences, North South University, Bangladesh. Email: nusrat.subhan@northsouth.edu",
"article": 269
}
]
},
{
"id": 270,
"slug": "178-1696140481-determination-of-multi-drug-resistance-profile-of-isolated-gram-positive-and-gram-negative-bacteria-from-clinical-pus-samples-in-bangladesh",
"type": "original_article",
"manuscript_id": "178-1696140481",
"published": "2024-01-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/52/178-1696140481.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 178-189.",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Nobel FA, Ahammad H, et al. Determination of multi-drug resistance profile of isolated Gram-positive and Gram-negative bacteria from clinical pus samples in Bangladesh. J Adv Biotechnol Exp Ther. 2024; 7(1): 178-189.",
"title": "Determination of multi-drug resistance profile of isolated Gram-positive and Gram-negative bacteria from clinical pus samples in Bangladesh",
"abstract": "<p>Antimicrobial resistance (AMR) has been a very concerning issue with consistent rise on a global scale. The rapid spread of drug-resistant pathogenic bacteria is a serious public health concern in both developed and developing countries, including Bangladesh. This study aimed to determine the prevalence of antibiotic resistant pathogenic bacteria and their multi-drug resistance (MDR) rate in pus samples. A total of 891 pus positive samples were collected from Tangail, Bangladesh. The resulting bacterial isolates were confirmed by biochemical tests and gram staining to classify bacterial species into two large groups. Antimicrobial susceptibility tests were performed for the identified bacterial isolates using the disk diffusion method. <em>Escherichia coli</em> exhibited the highest resistance level (98.92%, n=92). Also, <em>Pseudomonas</em> spp. displayed a substantial resistance pattern at 92.66% (n = 341), <em>Proteus</em> spp. at 91.58% (n = 87), <em>Klebsiella</em> spp.<em> </em>at 87.5% (n = 56), and <em>Acinetobacter </em>spp. exhibiting complete resistance of 100% (n =6). The cumulative MDR trend for Gram-negative bacteria was significant (92.98%, n = 583). Conversely, Gram-positive bacteria demonstrated a robust resistance pattern as well. <em>Streptococcus </em>spp. displayed resistance in 66.66% (n = 2) of cases, and <em>Enterococcus faecalis</em> exhibited resistance in 92.23% (n = 95) of instances. While <em>Staphylococcus aureus</em> showed a high resistance level with 95.06% (n = 77) of isolates. The overall drug-resistant pattern for Gram-positive bacteria was substantial (87.5%, n = 231). As per the findings of this study, bacteria frequently encountered demonstrate a concerning prevalence of MDR, posing a significant challenge to public health. The outcomes of this research may contribute valuable insights for formulating evidence-based treatment strategies and underscore the critical need for early identification of drug-resistant bacteria. Finally, this imperative step may hold the potential to mitigate the disease burden effectively.</p>",
"DOI": "10.5455/jabet.2024.d16",
"views": 1256,
"downloads": 170,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/03/18/178-1696140481-Figure1.jpg",
"caption": "Figure 1. The frequency and percentage of gram-positive and gram-negative bacteria in the pus samples.",
"featured": true
}
],
"authors": [
{
"id": 1275,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Fahim Alam",
"family_name": "Nobel",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-7975-4555",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 270
},
{
"id": 1276,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Hasib",
"family_name": "Ahammad",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 270
},
{
"id": 1281,
"affiliation": [
{
"affiliation": "Department of Microbiology, Noakhali Science and Technology University, Noakhali, Bangladesh"
}
],
"first_name": "Sumita Rani",
"family_name": "Saha",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 270
},
{
"id": 1282,
"affiliation": [
{
"affiliation": "Department of Microbiology, Noakhali Science and Technology University, Noakhali, Bangladesh"
}
],
"first_name": "Mohammad",
"family_name": "Asaduzzaman",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 270
},
{
"id": 1283,
"affiliation": [
{
"affiliation": "North South University, Dhaka, Bangladesh"
}
],
"first_name": "Aanan",
"family_name": "Nashra",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 270
},
{
"id": 1277,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Ruksana Akter",
"family_name": "Jebin",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 270
},
{
"id": 1278,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Yeasmin",
"family_name": "Zahan",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 270
},
{
"id": 1279,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Mohammad",
"family_name": "Kamruzzaman",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 270
},
{
"id": 1284,
"affiliation": [
{
"affiliation": "Centre for Cancer cell Biology and Drug Discovery, Griffith University, Nathan, Brisbane, Queensland 4111, Australia"
}
],
"first_name": "Golap",
"family_name": "Babu",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 270
},
{
"id": 1280,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Mohammod Johirul",
"family_name": "Islam",
"email": "johir7479@gmail.com",
"author_order": 10,
"ORCID": "http://orcid.org/0000-0002-4808-1685",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammod Johirul Islam, PhD;\r\nMawlana Bhashani Science and Technology University, Santosh, Tangail, Bangladesh. Email: johir7479@gmail.com",
"article": 270
}
]
},
{
"id": 268,
"slug": "178-1694758820-effect-of-xylo-oligosaccharides-and-b-glucan-on-gut-microbiota-and-short-chain-fatty-acid-in-diabetic-rats",
"type": "original_article",
"manuscript_id": "178-1694758820",
"published": "2023-12-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/42/178-1694758820.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 167-177",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Zahara IE, Mahati E, et al. Effect of xylo-oligosaccharides and β-glucan on gut microbiota and short chain fatty acid in diabetic rats. J Adv Biotechnol Exp Ther. 2024; 7(1): 167-177",
"title": "Effect of xylo-oligosaccharides and β-glucan on gut microbiota and short chain fatty acid in diabetic rats",
"abstract": "<p>Obesity and type 2 diabetes mellitus (T2DM) have an impact on an imbalance in the number of microbiota (dysbiosis) which can cause disruption of glucose metabolism by inhibiting short chain fatty acid (SCFA). SCFA is produced by intestinal microbial fermentation of indigestible dietary fibre and it has beneficial role in T2DM. Xylo-oligosaccharides (XOS) and β-glucans can improve the gut the composition of microbiota with prebiotic capabilities. The objective of the study was to investigate the effect of XOS and β-glucan on the <em>Lactobacillus, Roseburia,</em> and SCFA in diabetic rats. A total of 28 Wistar rats were divided into 4 groups including control (C), diabetic (D), diabetic+XOS (DX), and diabetic+β-glucan (DBG). D, DX, and DBG groups were provided with a high-fat diet (HFD) for 14 days before receiving an injection of streptozotocin (STZ) (45 mg/kg BW) and nicotinamide (110 mg/kg BW). DX received XOS (450 mg/200 g BW), and DBG received β-glucan (450 mg/200 g BW) for 28 days. The blood glucose levels at DX and DBG showed a significant difference compared with the D gro diabetic rats. XOS did not change the number, while β-glucan increased the number of <em>Lactobacillus</em> <em>and Roseburia</em>. The XOS and β-glucan supplementation significantly increased SCFA levels compared to diabetic rats. These results suggest that XOS can improve blood glucose although it does not change the number of <em>Lactobacillus</em> and <em>Roseburia.</em> Meanwhile, β-glucan increases blood glucose, <em>Lactobacillus, Roseburia,</em> and total SCFA in diabetic rats.<strong> </strong></p>",
"DOI": "10.5455/jabet.2024.d15",
"views": 1413,
"downloads": 161,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/14/178-1694758820-Figure1.jpg",
"caption": "Figure 1. Effect of XOS and β-Glucan on blood glucose of HFD-STZ-induced diabetic rats. Initial glucose level was the level when selecting experimental animals for inclusion criteria; Pre-treatment glucose levels were glucose levels in rats after being induced by STZ and NA; Post-treatment glucose levels were glucose levels after being induced by STZ and NA and after being supplemented with XOS and β-glucan for 28 days. C = control, healthy group; D = control negative (diabetic) HFD + STZ + NA; DX = Diabetic + XOS 450 mg/ 200 g BW; DBG= Giving HFD + STZ + NA + β-glucan 450 mg/200g. The chart shows the results of the post-hoc LSD test, which * indicated significant differences blood glucose between C groups (p < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/14/178-1694758820-Figure2.jpg",
"caption": "Figure 2. Effect of XOS and β-glucan on the number of Lactobacillus and Roseburia after intervention in rats. According to the results of a one-way ANOVA test, there was no significant difference in Lactobacillus between groups. In additions, the number of Roseburia according to the result of Kruskall Wallis show there is a significant difference, the chart shows the results of post hoc using Mann Whitney U test. C = control, healthy group; D = control negative (diabetic) HFD + STZ + NA; DX = Diabetic + XOS 450 mg/ 200 g BW; DBG= Giving HFD + STZ + NA + β-glucan 450 mg/200g. * = significant differences in the number of Roseburia between C groups and # (p<0.05) significant differences between DX groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/14/178-1694758820-Figure3.jpg",
"caption": "Figure 3. Effect of XOS and β-glucan on SCFA compositions after intervention in rats. The chart shows the results of the post-hoc LSD test, which indicated significant differences in SCFA compositions between groups (p < 0.05). C = control, healthy group; D = control negative (diabetic) HFD + STZ + NA; DX = Diabetic + XOS 450 mg/ 200 g BW; DBG= Giving HFD + STZ + NA + β-glucan 450 mg/200g BW; * = indicates significant differences between C groups.",
"featured": true
}
],
"authors": [
{
"id": 1259,
"affiliation": [
{
"affiliation": "Department of Nutrition, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Iftita Elok",
"family_name": "Zahara",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 268
},
{
"id": 1260,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutic, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Endang",
"family_name": "Mahati",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 268
},
{
"id": 1261,
"affiliation": [
{
"affiliation": "Department of Nutrition, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Mohammad",
"family_name": "Sulchan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 268
},
{
"id": 1262,
"affiliation": [
{
"affiliation": "Department of Nutrition, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Adriyan",
"family_name": "Pramono",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 268
},
{
"id": 1263,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Achmad Zulfa",
"family_name": "Juniarto",
"email": "zulfa_juniarto@fk.undip.ac.id",
"author_order": 5,
"ORCID": "https://orcid.org/0000-0002-5466-7690",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Achmad Zulfa Juniarto \r\nDepartment of Biology, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia; Email: zulfa_juniarto@fk.undip.ac.id",
"article": 268
}
]
},
{
"id": 267,
"slug": "178-1692693006-biochemical-and-histopathological-alterations-of-liver-and-kidney-of-japanese-quails-due-to-lead-toxicity-and-effects-of-vitamin-c",
"type": "original_article",
"manuscript_id": "178-1692693006",
"published": "2023-12-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/17/178-1692693006.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 157-166",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Hassan MA, Rahaman MT, et al. Biochemical and histopathological alterations of liver and kidney of Japanese quails due to lead toxicity and effects of vitamin C. J Adv Biotechnol Exp Ther. 2024; 7(1): 157-166.",
"title": "Biochemical and histopathological alterations of liver and kidney of Japanese quails due to lead toxicity and effects of vitamin C",
"abstract": "<p>Lead (Pb) is the most dangerous xenobiotic causing variety of negative impacts on human and animal health. Thus, the current study intended to investigate the effect of different doses of Pb on biochemical and histological abnormalities and the beneficial effects of vitamin C in the kidney and liver of quail. A total of<strong> </strong>72 male Japanese quails, aged 12 days were allotted in four groups with four replications. Control group (T<sub>0</sub>) received normal diet, group T<sub>1</sub> and T<sub>2</sub> received Pb at a dose of 70 mg and 700 mg/kg body weight (bw), respectively for 21 days, while group T<sub>3</sub> received 700 mg Pb /kg bw for 11 days followed by only vitamin C supplementation at a dose of 70 mg/kg bw without any Pb for next 10 days. After end of treatment, blood serum was collected, and the kidney and liver were taken for a histoarchitecture study. As expected, Pb-induced significant elevations of biochemical markers of liver and kidney injury were observed in different periods of treatment (group T<sub>3 </sub>at day 21) and decreased (except creatinine) following vitamin C supplementation in group T<sub>3 </sub>at day 21. Also, Pb treatment induced congestion, necrotic foci in different organs, reactive cells in central vein, degeneration of hepatocytes, necrosis in the portal area as well as renal tubular degeneration, all of which were slightly improved by vitamin C in group T<sub>3</sub>. Therefore, the findings suggest that the use of vitamin C could be a potential preventive agent against Pb-induced toxicity.</p>",
"DOI": "10.5455/jabet.2024.d14",
"views": 1353,
"downloads": 195,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/08/178-1692693006-Figure1.jpg",
"caption": "Figure 1. Some gross changes due to the treatment effect of Pb on different internal organs showing Mucosal erosion in Gizzard (A), accumulation of gas (B), enlargement of caeca (C), Pinpoint haemorrage in caecal tonsil (D), enlargement of liver (E) and lastly congestion in kidney and lung (F).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/08/178-1692693006-Figure2.jpg",
"caption": "Figure 2. Histological observation of liver in quail using H&E staining. A) Section of liver of control quail (Group T0) is showing normal histotexture of liver (40X). B) Section of liver of Pb exposed quail (Group T1) showing lymphocytic infiltration (black arrow), slightly congestion in central vein (blue arrow) with increased number of Kupffer cells (green arrow), and hepatic cell hypertrophy (white arrow) was found in the liver of Group T1 of quail (40X). C) Sections of the liver of quail fed with Pb (Group T2) showing huge Lymphocytic cell infiltration (black arrow) and necrosis in the portal area (PA) (black box), Cytolysis of hepatocyte (blue arrow), dilatation of bile canaliculi (red arrow) (40X). D) Sections of liver of quail of (Group T3) showing Less amount of reactive cells infiltrations (blue arrow) and slightly necrosis formation (black arrow), less hepatic cells disarrangement (green arrow), and preservation of normal histotexture of liver cell (40X). Scale bar: Scale bar: 1 μm(40X).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/08/178-1692693006-Figure3.jpg",
"caption": "Figure 3. Histological observation in the kidney of quails using H&E staining. Normal appearance of kidney was found in the control group, T0 (A, 40X), Whereas increase reactive cell infiltration (black arrow) and necrosis on renal tubule (blue arrow) was found in T1 group (B, 40X). On the other hand, screening huge reactive cell infiltration (black arrow) and necrosis on renal tubule (blue arrow), shrinkage of glomerulus (yellow arrow) was found in T2 group (C, 40X ) whereas less reactive cell infiltration (black arrow) and less necrosis on renal tubule (blue arrow) was observed in T3 group (D, 40X). Scale bar: 1 μm (40X).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/08/178-1692693006-Figure4.jpg",
"caption": "Figure 4. Effect of different doses of Pb and antioxidative effects of vitamin C supplementation on liver and kidney function biomarkers in quail. Quail kept untreated with Pb (Group T0 ), Group T1 (70mg Pb /kg bw), GroupT2 (700mg Pb /kg bw), Group T3 (700 mg Pb /kg bw ) for 10 days followed by only vitamin C supplementation at a dose of 70 mg/kg bw without any Pb for next 10 days and the values of (A) ALT, (B) AST, and (C) Serum creatinine of four treatment groups and three different period ( d0,d10 and d21) were measured. Data indicate mean ±SEM of 4 replicates. Single factor analysis of variance was done to analyze the statistical differences between treatment groups and t-test was done for differences between the period of treatment, here, *statistically significant at 5% level (P≤0.05). Each group contains 18 (eighteen) quails.",
"featured": false
}
],
"authors": [
{
"id": 1254,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Abu",
"family_name": "Hassan",
"email": "dr.abuhassan2015dvm@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-3247-4943",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Abu Hassan; Dept. of Anatomy and Histology, \r\nHajee Mohammad Danesh Science and Technology University,\r\nDinajpur-5200, Bangladesh. Email: dr.abuhassan2015dvm@gmail.com",
"article": 267
},
{
"id": 1255,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Toheder",
"family_name": "Rahaman",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 267
},
{
"id": 1256,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Najmul Hassan",
"family_name": "Parvez",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 267
},
{
"id": 1257,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Khadija Al",
"family_name": "Ferdous",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 267
},
{
"id": 1258,
"affiliation": [
{
"affiliation": "Department of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
},
{
"affiliation": "School of Veterinary Medicine, Texas Tech University, 7671 Evans Dr., Amarillo, TX 79106, USA"
}
],
"first_name": "Sumon",
"family_name": "Sarkar",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 267
}
]
},
{
"id": 266,
"slug": "178-1693146466-exploring-the-role-of-autoantibodies-in-iraqi-females-with-polycystic-ovary-syndrome",
"type": "original_article",
"manuscript_id": "178-1693146466",
"published": "2023-12-04",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/22/178-1693146466.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 147-156",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Al-Karawi AS, Kadhim AS. Exploring the role of autoantibodies in Iraqi females with polycystic ovary syndrome. J Adv Biotechnol Exp Ther. 2024; 7(1): 147-156.",
"title": "Exploring the role of autoantibodies in Iraqi females with polycystic ovary syndrome",
"abstract": "<p>Polycystic ovary syndrome (PCOS) is a prevalent endocrinopathy that affects 5-10% of women during their reproductive age. Recent research suggests a potential link between autoantibodies and PCOS development. The current study aimed to investigate the relationship between autoantibodies and PCOS. One hundred and fifty female patients suffered from PCOS aged between 20 and 35 years participated in this study as matched with healthy control. The result showed a significantly higher concentrations of anti-thyroid peroxidase (TPO) and anti-thyroglobulin (TG) autoantibodies in the patients compared to the healthy control group. Moreover, the concentrations of IgM and IgG for anti-cardiolipin (ACL) were significantly higher in the patients than in the control group. These increments in ACL antibodies in patients suggested a risk factor for PCOS. Conversely, no significant correlation was found in case of β-2 glycoprotein I (β2GPI) antibodies and lupus in patients. Serum amyloid A (SAA) and high-sensitivity C-reactive protein (hs-CRP) were significantly higher in patients. Procalcitonin (PCT) was also highly significant in patients compared to the controls. Lastly, the concentration of total IgG antibodies was significantly higher in patients than in controls. In conclusion, high concentrations of autoantibodies in patients suggest a risk factor and positive associations with PCOS in females.</p>",
"DOI": "10.5455/jabet.2024.d13",
"views": 1272,
"downloads": 194,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/03/178-1693146466-Figure1.jpg",
"caption": "Figure 1. Comparison of thyroid antibodies levels (TPO and TG, AU/ml) between PCOS patients and controls. Here, 150 women aged 20 to 35 with PCOS and control group of 150 healthy individuals matched in age to PCOS group was included. Luminescence immunoassay was used to measure the thyroid antibodies. Data are presented as mean±SE; *p<0.05 vs. control. Anti-TG: Anti-thyroglobulin antibodies, and Anti-TPO: Anti-thyroperoxidase antibodies.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/03/178-1693146466-Figure2.jpg",
"caption": "Figure 2. Comparison of inflammatory biomarker levels in patients. Here, 150 women aged 20 to 35 with PCOS and control group of 150 healthy individuals matched in age to PCOS group was included. The nephelometry method was used to measure the inflammatory biomarker levels. Data are presented as mean±SE, *p<0.05 vs. control. hs-CRP: High-Sensitivity C-Reactive Protein, SAA: Serum amyloid A, and PCT: Procalcitonin.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/03/178-1693146466-Figure3.jpg",
"caption": "Figure 3. The levels of total antibodies such as IgM, IgA, and IgG (AU/ml) in study patients. Here, 150 women aged 20 to 35 with PCOS and control group of 150 healthy individuals matched in age to PCOS group was included. The nephelometry method was used to measure the total antibodies (IgM, IgA, IgG) levels. Data are presented as mean±SE, *p<0.05 vs. control. Ig(M, A, G): Immunoglobulin (M, A, G).",
"featured": false
}
],
"authors": [
{
"id": 1252,
"affiliation": [
{
"affiliation": "Department of Microbiology, College of Science, Mustansiriyah University, Baghdad, Iraq"
}
],
"first_name": "Abdullah Salim",
"family_name": "Al-Karawi",
"email": "Abdullah.S.Shaker@uomustansiriyah.edu.iq",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-5532-7508",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Abdullah Salim Al-Karawi\r\nDepartment of Microbiology, College of Science, Mustansiriyah University, Baghdad, Iraq. Email: Abdullah.S.Shaker@uomustansiriyah.edu.iq",
"article": 266
},
{
"id": 1253,
"affiliation": [
{
"affiliation": "Department of Science, College of Basic Education, Wasit University, Wasit, Iraq"
}
],
"first_name": "Ali Saad",
"family_name": "Kadhim",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-7456-2178",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 266
}
]
},
{
"id": 265,
"slug": "178-1696521612-high-intensity-interval-training-increases-ampk-and-glut4-expressions-via-fgf21-in-skeletal-muscles-of-diabetic-rats",
"type": "original_article",
"manuscript_id": "178-1696521612",
"published": "2023-11-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/28/178-1696521612.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 136-146",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Kartinah NT, Rusli H, et al. High-intensity interval training increases AMPK and GLUT4 expressions via FGF21 in skeletal muscles of diabetic rats. J Adv Biotechnol Exp Ther. 2024; 7(1): 136-146",
"title": "High-intensity interval training increases AMPK and GLUT4 expressions via FGF21 in skeletal muscles of diabetic rats",
"abstract": "<p>In type 2 diabetes, a decrease in expression of glucose transporter type 4 (GLUT4) results in decreased glucose uptake while fibroblast growth factor 21 (FGF21) promotes glucose uptake. The liver and other organs, such as the muscles, produce FGF21. Physical activity increases FGF21 secretion from skeletal muscles. FGF21 is thought to promote glucose uptake during exercise by activating AMP-activated protein kinase (AMPK). Thus, the goal of this study is to determine how high-intensity interval training (HIIT) affects glucose uptake via FGF21 signaling in diabetic rats. Twenty-four eight-week-old male Wistar rats were randomly divided into four categories including Control (Con), DM intervention (ConDM), DM group undergoing continuous training (DM-CT), and DM group undergoing high-intensity interval training (DM-HIIT). The trainings in the exercise groups (DM-CT and DM-HIIT) were performed six days a week for six weeks. We analyzed FGF21 levels in serum and muscles by ELISA. Measurement of phosphorylated AMPK in muscles used ELISA, and that of mRNA GLUT4 expression used RT-PCR. Following HIIT interventions, the glucose level in plasma was closer to normal level. An increase in FGF21 levels aided in the reduction in blood glucose. The HIIT group had significantly higher FGF21 levels in serum and muscles. An increase in FGF21 was followed by an increase in AMPK phosphorylation and GLUT4 expression in the HIIT group. In diabetic rats, HIIT promotes glucose uptake via FGF21, which activates AMPK and increases GLUT4 expression.</p>",
"DOI": "10.5455/jabet.2024.d12",
"views": 1332,
"downloads": 169,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/02/178-1696521612-Figure1.jpg",
"caption": "Figure 1. Effect of exercise on blood sugar levels in STZ rats. A) Average blood sugar levels of rats after STZ injection, before exercise. B) Average blood sugar levels of rats after six weeks of physical exercise. The graph is presented in the form of mean ± SEM. There are significant distinctions. **p < 0.01, ****p < 0.0001. Con, without intervention; ConDM, DM intervention; DM-CT, DM rats undergoing continuous training; DM-HIIT, DM rats undergoing high-intensity interval training.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/02/178-1696521612-Figure2.jpg",
"caption": "Figure 2. Average skeletal muscle GLUT4 expression after six weeks of CT and HIIT exercise. The graph is presented in the form of mean ± SEM. There are significant distinctions. ***p < 0.001, ****p < 0.0001. Con, without intervention; ConDM, DM intervention; DM-CT, DM rats undergoing continuous training; DM-HIIT, DM rats undergoing high-intensity interval training.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/02/178-1696521612-Figure3.jpg",
"caption": "Figure 3. Phosphorylation-AMPK levels of skeletal muscles after six weeks of CT and HIIT exercise. The graph is presented in the form of mean ± SEM. There are significant distinctions. *p < 0.05, ***p < 0.001, ****p < 0.0001. Con, without intervention; ConDM, DM intervention; DM-CT, DM rats undergoing continuous training; DM-HIIT, DM rats undergoing high-intensity interval training.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/02/178-1696521612-Figure4.jpg",
"caption": "Figure 4. FGF21 levels of skeletal muscles after six weeks of CT and HIIT exercise. The graph is presented in the form of mean ± SEM. There are significant distinctions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001. Con, without intervention; ConDM, DM intervention; DM-CT, DM rats undergoing continuous training; DM-HIIT, DM rats undergoing high-intensity interval training.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/02/178-1696521612-Figure5.jpg",
"caption": "Figure 5. Serum FGF21 levels after six weeks of CT and HIIT exercise. The graph is presented in the form of mean ± SEM. There are significant distinctions. ***p < 0.001, ****p < 0.0001. Con, without intervention; ConDM, DM intervention; DM-CT, DM rats undergoing continuous training; DM-HIIT, DM rats undergoing high-intensity interval training.",
"featured": false
}
],
"authors": [
{
"id": 1244,
"affiliation": [
{
"affiliation": "Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia"
}
],
"first_name": "Neng Tine",
"family_name": "Kartinah",
"email": "tine.kartinah@ui.ac.id",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-0028-749X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Neng Tine Kartinah; Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia Email: tine.kartinah@ui.ac.id",
"article": 265
},
{
"id": 1245,
"affiliation": [
{
"affiliation": "Department of Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia"
}
],
"first_name": "Hardiyanti",
"family_name": "Rusli",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-8603-904X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 265
},
{
"id": 1246,
"affiliation": [
{
"affiliation": "Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia"
}
],
"first_name": "Ermita I.Ibrahim",
"family_name": "Ilyas",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 265
},
{
"id": 1247,
"affiliation": [
{
"affiliation": "Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia"
}
],
"first_name": "Trinovita",
"family_name": "Andraini",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Trinovita Andraini; Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia.",
"article": 265
},
{
"id": 1248,
"affiliation": [
{
"affiliation": "Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia"
}
],
"first_name": "Nurul",
"family_name": "Paramita",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 265
},
{
"id": 1249,
"affiliation": [
{
"affiliation": "Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia"
}
],
"first_name": "Dewi Irawati S.",
"family_name": "Santoso",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 265
},
{
"id": 1250,
"affiliation": [
{
"affiliation": "Department of Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia"
}
],
"first_name": "Brilliant Cahya",
"family_name": "Puspasari",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 265
},
{
"id": 1251,
"affiliation": [
{
"affiliation": "Department of Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jalan Salemba Raya No. 6 Jakarta Pusat 10403, Indonesia"
}
],
"first_name": "Julfiana",
"family_name": "Mardhatillah",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 265
}
]
},
{
"id": 264,
"slug": "178-1691244361-hemolytic-antioxidant-and-phospholipase-a2-neutralization-efficacy-of-asparagus-racemosus-withania-somnifera-syzygium-cumini-psidium-guajava-basella-alba-morus-indica-morus-laevigata-and-morus-latifolia",
"type": "original_article",
"manuscript_id": "178-1691244361",
"published": "2023-11-16",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/01/178-1691244361.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 118-135",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Joty FA, Hasan MM, et al. Hemolytic, antioxidant, and phospholipase A2 neutralization efficacy of Asparagus racemosus, Withania somnifera, Syzygium cumini, Psidium guajava, Basella alba, Morus indica, Morus laevigata, and Morus latifolia. J Adv Biotechnol Exp Ther. 2024; 7(1): 118-135",
"title": "Hemolytic, antioxidant, and phospholipase A2 neutralization efficacy of <span>Asparagus racemosus, Withania somnifera, Syzygium cumini, Psidium guajava, Basella alba, Morus indica, Morus laevigata, </span>and <span>Morus latifolia</span>",
"abstract": "<p>Bangladesh is abundant in medicinal plants. The bioactive compounds in medicinal plants provide novel therapeutic opportunity of improving human health. But the benefit-risk balance is usually less considered during formulation of traditional medicine. However, the plants included in this study were not studied before regarding their hemolytic and anti-venom properties. Thus, the current study aimed to evaluate and compare the hemolytic activity, antioxidant activity, and <em>phospholipase A<sub>2</sub></em> (PLA<sub>2</sub>)<sub> </sub>enzyme neutralization efficacy of aqueous and methanolic extracts of eight known medicinal plants including <em>Asparagus racemosus </em>(root), <em>Withania somnifera </em>(root), <em>Syzygium cumini </em>(leaf), <em>Psidium guajava </em>(leaf), <em>Basella alba </em>(leaf and seed), <em>Morus indica </em>(leaf), <em>M. laevigata </em>(leaf), and <em>M. latifolia </em>(leaf). The results of the hemolytic activity test suggested that the methanolic extracts of <em>S. cumini</em>, <em>P. guajava</em>, <em>B. alba </em>(leaf and seed), <em>M. indica</em>, <em>M. laevigata</em>, and <em>M. latifolia</em> showed significantly higher hemolytic activity compared to their aqueous extracts. The methanolic extracts of <em>A. racemosus</em>, <em>W. somnifera</em>, <em>P. guajava</em>, <em>M. laevigata</em>, and <em>M. latifolia</em> showed remarkable antioxidant activity. Moreover, the results of the PLA<sub>2 </sub>enzyme neutralization activity test revealed that both aqueous and methanolic extracts of <em>P. guajava</em>, <em>S. cumini</em>, and <em>B. alba </em>(leaf) have significant PLA<sub>2</sub> neutralization capacity. Altogether, the aqueous extracts of all plants, except <em>A. racemosus</em> root, have ignorable hemolytic effect. Both of the aqueous and methanolic extracts of <em>M. latifolia</em> showed the maximum antioxidant activity. Additionally, <em>P. guajava</em> and <em>S. cumini</em> leaf aqueous extracts have promising PLA<sub>2 </sub>inhibitory effect which may become the potential source of bioactive compounds for anti-venom drug development. </p>",
"DOI": "10.5455/jabet.2024.d11",
"views": 1170,
"downloads": 101,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/16/178-1691244361-Figure1.jpg",
"caption": "Figure 1. Hemolytic activity of aqueous extracts of experimental samples. (A) A. racemosus (root); (B) W. somnifera (root); (C) S. cumini (leaf); (D) P. guajava (leaf); (E) B. alba (leaf); (F) B. alba (seed); (G) M. indica (leaf); (H) M. laevigata (leaf); (I) M. latifolia (leaf). SDS = sodium dodecyl sulfate. Data are presented as mean±SD, n = 3. ns = non-significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/16/178-1691244361-Figure2.jpg",
"caption": "Figure 2. Hemolytic activity of methanolic extracts of experimental samples. (A) A. racemosus (root); (B) W. somnifera (root); (C) S. cumini (leaf); (D) P. guajava (leaf); (E) B. alba (leaf); (F) B. alba (seed); (G) M. indica (leaf); (H) M. laevigata (leaf); (I) M. latifolia (leaf). SDS = sodium dodecyl sulfate. Data are presented as mean±SD, n = 3. ns = non-significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/16/178-1691244361-Figure3.jpg",
"caption": "Figure 3. Antioxidant activity of aqueous extracts of experimental samples. (A) A. racemosus (root); (B) W. somnifera (root); (C) S. cumini (leaf); (D) P. guajava (leaf); (E) B. alba (leaf); (F) B. alba (seed); (G) M. indica (leaf); (H) M. laevigata (leaf); (I) M. latifolia (leaf); (J) Ascorbic acid (standard). Data are presented as mean±SD, n = 3.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/16/178-1691244361-Figure4.jpg",
"caption": "Figure 4. Antioxidant activity of methanolic extracts of experimental samples. (A) A. racemosus (root); (B) W. somnifera (root); (C) S. cumini (leaf); (D) P. guajava (leaf); (E) B. alba (leaf); (F) B. alba (seed); (G) M. indica (leaf); (H) M. laevigata (leaf); (I) M. latifolia (leaf); (J) Ascorbic acid (standard). Data are presented as mean±SD, n = 3.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/16/178-1691244361-Figure5.jpg",
"caption": "Figure 5. PLA2 enzyme neutralization efficacy of aqueous extracts of experimental samples. (A) A. racemosus (root); (B) W. somnifera (root); (C) S. cumini (leaf); (D) P. guajava (leaf); (E) B. alba (leaf); (F) B. alba (seed); (G) M. indica (leaf); (H) M. laevigata (leaf); (I) M. latifolia (leaf). Data are presented as mean±SD, n = 3. ns = non-significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/16/178-1691244361-Figure6.jpg",
"caption": "Figure 6. PLA2 enzyme neutralization efficacy of methanolic extracts of experimental samples. (A) A. racemosus (root); (B) W. somnifera (root); (C) S. cumini (leaf); (D) P. guajava (leaf); (E) B. alba (leaf); (F) B. alba (seed); (G) M. indica (leaf); (H) M. laevigata (leaf); (I) M. latifolia (leaf). Data are presented as mean±SD, n = 3. ns = non-significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.",
"featured": false
}
],
"authors": [
{
"id": 1238,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Farhana Afroz",
"family_name": "Joty",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0002-7719-3181",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 264
},
{
"id": 1239,
"affiliation": [
{
"affiliation": "Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan"
},
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Bangladesh"
}
],
"first_name": "Md. Mahmudul",
"family_name": "Hasan",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-2489-6772",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 264
},
{
"id": 1240,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Bangladesh"
}
],
"first_name": "Md. Sohanur Rahman",
"family_name": "Sohan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 264
},
{
"id": 1241,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Bangladesh"
}
],
"first_name": "Md. Jahangir",
"family_name": "Alam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 264
},
{
"id": 1242,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Bangladesh"
}
],
"first_name": "Md. Abu",
"family_name": "Reza",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0002-3146-2772",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 264
},
{
"id": 1243,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Bangladesh"
}
],
"first_name": "Zennat",
"family_name": "Ferdousi",
"email": "ferdousi_gen@ru.ac.bd",
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-1451-2934",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Zennat Ferdousi, PhD;\r\nMolecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Bangladesh. Email: ferdousi_gen@ru.ac.bd",
"article": 264
}
]
},
{
"id": 263,
"slug": "178-1695566469-recent-advances-in-peptide-based-nanovaccines-for-re-emerging-and-emerging-infectious-diseases",
"type": "review_article",
"manuscript_id": "178-1695566469",
"published": "2023-11-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/29/178-1695566469.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1):106-117",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Orosco FL. Recent advances in peptide-based nanovaccines for re-emerging and emerging infectious diseases. J Adv Biotechnol Exp Ther. 2024; 7(1):106-117.",
"title": "Recent advances in peptide-based nanovaccines for re-emerging and emerging infectious diseases",
"abstract": "<p>Peptide-based nanovaccines have emerged as promising strategies for combating re-emerging and emerging infectious diseases. They exhibit excellent immunogenicity and therapeutic potential. They have shown the ability to elicit robust immune responses, such as activation of antigen-presenting cells, induction of antibodies and T-cell responses, and generation of memory immune cells. This comprehensive review article aims to provide a thorough overview of recent advances in the field, including immunological mechanisms, structural design approaches, and utilization of various nanomaterials. Overall, peptide-based nanovaccines hold great promise in combating infectious diseases. Precise design and assembly of targeted and tailored immune responses will enable effective prevention, treatment, and long-term protection. Further research is needed to optimize their efficacy, safety, and clinical translation. The knowledge gained from these studies will pave the way for a future with more effective immunotherapeutic interventions against infectious diseases.</p>",
"DOI": "10.5455/jabet.2024.d10",
"views": 1997,
"downloads": 212,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/32/10/178-1695566469-Figure1.jpg",
"caption": "Figure 1. Activation of cellular and humoral immune responses by nanovaccines. The cellular and humoral immune responses to nanovaccines are initiated when the nanovaccine enters the epithelium to activate dendritic cells. Dendritic cells migrate to lymph nodes to prime T and B cell which will attack viruses and virus-infected cells.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/32/10/178-1695566469-Figure2.jpg",
"caption": "Figure 2. Nanovaccines can be formulated by physical adsorption, chemical conjugation, encapsulation, or physical mixing of antigens with nanoparticles.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/32/10/178-1695566469-Figure3.jpg",
"caption": "Figure 3. Types of nanovaccines. The different types of nannovaccines used for viral infections include vaccines conjugated to polymeric, liposome, and inorganic nanoparticles.",
"featured": false
}
],
"authors": [
{
"id": 1237,
"affiliation": [
{
"affiliation": "Virology and Vaccine Institute of the Philippines Program, Industrial Technology Development Institute, Department of Science and Technology, Bicutan, Taguig City, Philippines"
},
{
"affiliation": "S&T Fellows Program, Department of Science and Technology, Bicutan, Taguig City, Philippines"
}
],
"first_name": "Fredmoore L.",
"family_name": "Orosco",
"email": "orosco.fredmoore@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-8861-7923",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Fredmoore Orosco, PhD; Virology and Vaccine Institute of the Philippines Program, Industrial Technology Development Institute, Department of Science and Technology, Bicutan, Taguig City, Philippines.\r\nEmail: orosco.fredmoore@gmail.com",
"article": 263
}
]
},
{
"id": 262,
"slug": "178-1694096859-identification-of-polymorphisms-in-melanocortin-1-receptor-gene-and-their-association-with-coat-color-variants-in-black-bengal-goat",
"type": "original_article",
"manuscript_id": "178-1694096859",
"published": "2023-11-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/13/178-1694096859.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 95-105",
"academic_editor": "Mohammad Nazrul Islam, PhD; Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh",
"cite_info": "Azam S, Mou MA, et al. Identification of polymorphisms in melanocortin 1 receptor gene and their association with coat color variants in Black Bengal goat. J Adv Biotechnol Exp Ther. 2024; 7(1): 95-105.",
"title": "Identification of polymorphisms in melanocortin 1 receptor gene and their association with coat color variants in Black Bengal goat",
"abstract": "<p>In domestic goats, coat color is genetically determined as an important character and has an economic value. The aim of this study was to identify polymorphisms in the entire coding regions of the melanocortin 1 receptor (MC1R) gene and their possible associations with coat color variants of the Black Bengal goat (BBG) of Bangladesh. Forty blood samples representing five distinct color variants of BBG (Solid Black, Toggenburg, Dutch Belt, White and Brown) were collected from Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh. Two PCR amplicons that harbored entire coding region were sequenced both forward and reverse directions. Multiple sequence alignment identified four single nucleotide polymorphisms (SNPs) based on <em>Capra hircus</em> reference sequence (MT186778.1). Among them, the c.183C>T (p.61A) polymorphism was identified as synonymous type and the remaining three were non-synonymous in nature (c.676A>G, c.748G>T, and c.801C>G), causing amino acid substitutions as p.K226E, p.V250F, and p.C267W, respectively. Twelve haplotypes were defined by four substitutions where four haplotypes (Hap6, Hap7, Hap8, and Hap9) were found white-color goat-specific involving c.801C>G mutation that might be associated with white coat color phenotype of BBG. The UPGMA phylogenetic tree revealed two separate clusters, one of which included all color variants of BBG and major domestic goat populations (<em>Capra hircus</em>) from various countries. The haplotype sharing features suggest that the coat color phenotypes of BBG were not solely attributable to the MC1R gene. However, more detailed investigation with large samples from diverse populations across the country is required to draw a precise conclusion.</p>",
"DOI": "10.5455/jabet.2024.d09",
"views": 1434,
"downloads": 116,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/08/178-1694096859-Figure1.jpg",
"caption": "Figure 1. Photos of investigated BBG with different coat colors; (A) Solid Black, (B) Toggenburg, (C) Brown, (D) Dutch belt and (E) White.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/08/178-1694096859-Figure2.jpg",
"caption": "Figure 2. Identified polymorphisms of MC1R gene (sequence electropherograms for the four SNPs are reported both for the two homozygous and the heterozygous genotypes except c.801C>G).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/08/178-1694096859-Figure3.jpg",
"caption": "Figure 3. Alignment of protein coding sequence of MC1R gene of BBG of Bangladesh and a Capra hircus reference sample (MT186778.1). The exact amino acid position is highlighted with red color in the Capra hircus protein sequence. The abbreviations REF, BB, WH, TG, DB and BR represent reference protein sequence along with Solid Black, White, Toggenburg, Dutch Belt, Brown color variants of BBG of Bangladesh.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/08/178-1694096859-Figure4.jpg",
"caption": "Figure 4. UPGMA phylogenetic tree based on maximum composite likelihood method applying 10000 bootstrap replications. The entire coding sequences of MC1R gene from five coat color variants of Black Bengal goat as Solid black (BL), Toggenburg (TG), Dutch belt (DB), Brown (BR) and White (WH) along with goat breeds of different countries (Capra hircus) are included as reference samples. Coding sequences of MC1R gene of sheep, pig, horse, zebu cattle and buffalo samples are included as outgroups.",
"featured": false
}
],
"authors": [
{
"id": 1230,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Shahala",
"family_name": "Azam",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0005-9617-5802",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 262
},
{
"id": 1231,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Monira Akter",
"family_name": "Mou",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0009-0005-3456-4140",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 262
},
{
"id": 1232,
"affiliation": [
{
"affiliation": "Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
}
],
"first_name": "Nure Hasni",
"family_name": "Desha",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-3221-249X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 262
},
{
"id": 1233,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Forhad Ahmed",
"family_name": "Hridoy",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0009-0009-3994-3130",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 262
},
{
"id": 1234,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Munir",
"family_name": "Hossain",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 262
},
{
"id": 1236,
"affiliation": [
{
"affiliation": "Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
}
],
"first_name": "Sadek",
"family_name": "Ahmed",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-9680-7372",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 262
},
{
"id": 1235,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mohammad Shamsul Alam",
"family_name": "Bhuiyan",
"email": "msabhuiyan.abg@bau.edu.bd",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0003-1525-2182",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Shamsul Alam Bhuiyan, PhD; \r\nDepartment of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh\r\nEmail: msabhuiyan.abg@bau.edu.bd",
"article": 262
}
]
},
{
"id": 261,
"slug": "178-1690020027-assessing-the-efficacy-of-different-semen-extenders-on-post-thaw-quality-of-bangladeshi-buffalo-sperm-under-a-manual-cryopreservation-protocol",
"type": "original_article",
"manuscript_id": "178-1690020027",
"published": "2023-11-04",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/06/178-1690020027.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 83-94",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Kabir MA, Hossain SMJ, et al. Assessing the efficacy of different semen extenders on post-thaw quality of Bangladeshi buffalo sperm under a manual cryopreservation protocol. J Adv Biotechnol Exp Ther. 2024; 7(1): 83-94",
"title": "Assessing the efficacy of different semen extenders on post-thaw quality of Bangladeshi buffalo sperm under a manual cryopreservation protocol",
"abstract": "<p>Cryopreservation has been used extensively for cattle in Bangladesh, however no study was conducted on the cryopreservation of buffalo sperm in Bangladesh. Thus, the current study aimed to evaluate the suitability of different semen extenders for improving the post-thaw quality of Bangladeshi buffalo sperm under a manual cryopreservation protocol. The manual cryopreservation protocol was compared and optimized with a commercial biofreezer protocol. Then, the efficacy of different diluters was evaluated using the optimized manual cryopreservation protocol. Meanwhile, the post-thaw sperm quality in terms of motility and morphology was evaluated by computer assisted sperm analyzer during the optimization process. During manual cryopreservation, the first cooling from 37 °C to 5 °C was done in an equilibration chamber and the second cooling from 5 °C to -120 °C in a Styrofoam box using liquid nitrogen vapor from different distances (0.5, 1.5, 1.6, 2 and 3 inches). Simultaneously, another batch of sperm was cryopreserved using a programmable freezer. The highest number of motile sperms (62.67±1.12; P<0.01) and progressive motility (38.97±1.10; P< 0.001) was observed at 1.6 inches above liquid nitrogen, which were similar to the results obtained from automated biofreezer protocol (65.94±4.65 and 45.54 ± 3.64, respectively). To evaluate the semen extenders’ efficacy, one locally developed Tris-fructose-egg yolk-based diluter and two commercial diluters (Andromed, and Triladyl) were used in the freezing of buffalo sperm. The highest recovery and conception rates were observed in sperm diluted with tris-fructose-egg yolk-based (TFE) (82.4% and 80%, respectively). Therefore, it is suggested that this manual cryopreservation protocol and the TFE diluter could be a suitable and inexpensive alternative for Bangladeshi buffalo sperm considering post-thaw sperm quality and fertility.</p>",
"DOI": "10.5455/jabet.2024.d08",
"views": 1250,
"downloads": 114,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/24/07/178-1690020027-Figure_1.jpg",
"caption": "Figure 1. Comparison of motility and morphology of cryopreserved sperm analyzed by CASA (200×magnification). A) Pre-frozen semen, B) Nitrogen vapor technique (4cm above nitrogen), and C) Commercial freezing technique. The green lines indicated the motility of sperm. Sperms with light blue tracks are progressively motile. The red dots indicated static sperms while the yellow color indicated sperms that entered or left too early within the analysis frame.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/24/07/178-1690020027-Figure_2.jpg",
"caption": "Figure 2. Effect of different diluters on sperm motility and morphology analyzed by CASA (200×magnification). A) Pre-forzen, B) TFE, C) Triladyl, and D) Andromed. The green lines indicated the motility of sperm. Sperms with light blue tracks are progressively motile. The red dots indicated static sperms, while the yellow color indicated sperms that entered or were left too early within the analysis frame.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/24/07/178-1690020027-figure3.jpg",
"caption": "Figure 3. The recovery rate of cryopreserved semen with different diluters. Different letters (a, b) indicate differences among freezing groups (p<0.05).",
"featured": false
}
],
"authors": [
{
"id": 1224,
"affiliation": [
{
"affiliation": "Biotechnology Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
},
{
"affiliation": "College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China"
}
],
"first_name": "Md. Ahsanul",
"family_name": "Kabir",
"email": "rupom353@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-3171-8659",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Ahsanul Kabir\r\nBiotechnology Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh. Email: rupom353@gmail.com",
"article": 261
},
{
"id": 1225,
"affiliation": [
{
"affiliation": "Biotechnology Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
}
],
"first_name": "S. M. Jahangir",
"family_name": "Hossain",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 261
},
{
"id": 1226,
"affiliation": [
{
"affiliation": "Buffalo Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
}
],
"first_name": "Gautam Kumar",
"family_name": "Deb",
"email": "debgk2003@blri.gov.bd",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Gautam Kumar Deb, PhD;\r\nBuffalo Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh. Email: debgk2003@blri.gov.bd",
"article": 261
},
{
"id": 1227,
"affiliation": [
{
"affiliation": "Biotechnology Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
}
],
"first_name": "Shahanaj Ferdousi",
"family_name": "Shejuty",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 261
},
{
"id": 1228,
"affiliation": [
{
"affiliation": "Biotechnology Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
}
],
"first_name": "Dipa",
"family_name": "Das",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 261
},
{
"id": 1229,
"affiliation": [
{
"affiliation": "Biotechnology Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
},
{
"affiliation": "Department of Genetic Engineering & Biotechnology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh"
}
],
"first_name": "Abdullah Al",
"family_name": "Noman",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0003-0615-744X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 261
}
]
},
{
"id": 260,
"slug": "178-1694091881-analysis-of-b-casein-gene-for-a1-and-a2-allelic-variants-in-holstein-local-crossbred-cattle-of-bangladesh-using-allele-specific-pcr",
"type": "original_article",
"manuscript_id": "178-1694091881",
"published": "2023-10-29",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/41/178-1694091881.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 74-82",
"academic_editor": "Mohammad Nazrul Islam, PhD; Sher-e-Bangla Agricultural University, Bangladesh",
"cite_info": "Safa MA, Mou MA, et al. Analysis of β-casein gene for A1 and A2 allelic variants in Holstein-Local crossbred cattle of Bangladesh using allele-specific PCR. J Adv Biotechnol Exp Ther. 2024; 7(1): 74-82.",
"title": "Analysis of β-casein gene for A1 and A2 allelic variants in Holstein-Local crossbred cattle of Bangladesh using allele-specific PCR",
"abstract": "<p>β-casein (CSN2) is one of the important bovine milk proteins synthesized by β-casein gene. The aim of this study was to investigate the A1 and A2 allelic variants of CSN2 gene in Holstein-Friesian and Local (HF×L) crossbred cattle of Bangladesh. Allele specific polymerase chain reaction (AS-PCR) was carried out for genotyping of allelic variants. A total of 390 blood samples of HF×L crossbred cattle was included from government, military, and private dairy farms. Six different crossbred genotypes were identified in the studied population such as 50%HF×50%L, 62.5%HF×37.5%L, 68.75%HF×31.25%L, 75%HF×25%L, 81.25%HF×18.75%L, and 87.5%HF×12.5%L. The mean frequencies of the A1A1, A1A2, and A2A2 genotypes were 0.11, 0.48, and 0.41, respectively, while the corresponding frequencies of the A1 and A2 alleles were 0.34 and 0.66, respectively. The frequency of desirable A2A2 genotype reduced from 0.60 (50%HF×50%L) to 0.38 (87.5%HF×12.5%L) with the advancement of exotic HF inheritance from 50 to 87.5%. In contrast, the frequency of A1A1 and A1A2 genotypes increased proportionately with the progression of HF inheritance level. The undesirable A1A1 genotype frequency was found highest in the HF crossbreds of private dairy farms (0.13) compared to government (0.08) and military dairy farm (0.07) that signifies the availability of upper graded cows in the private dairy farms. Altogether, this study provides current scenario of A1 and A2 allelic variants of HF×L crossbreds of Bangladesh that could be utilized to design suitable breeding plans in crossbreeding programs for production of premium quality milk.</p>",
"DOI": "10.5455/jabet.2024.d07",
"views": 1333,
"downloads": 152,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/28/178-1694091881-Figure1.jpg",
"caption": "Figure 1. Allele specific genotyping of A1 and A2 allelic variants of β-casein gene. M = 100 bp size marker, line arrow indicates the expected fragment size. The samples representing 277 and 209 bp bands for the A1A1 genotype, 277 and 108 bp bands for the A2A2 genotype, and heterozygous individuals contain 277, 209, and 108 bp bands.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/28/178-1694091881-Figure2.jpg",
"caption": "Figure 2. Frequency distribution of CSN2 genotypes in different HF derived crossbred cattle populations. ᴬJawani et al. (2018), ᴮHaq et al. (2012), ᴻMumtaz et al. (2019), ᴲPatel et al. (2019), ᴷMalarmathi et al. (2014) and ᵂThis study.",
"featured": false
}
],
"authors": [
{
"id": 1218,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mahfuza Ahmed",
"family_name": "Safa",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0009-0002-7661-9718",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 260
},
{
"id": 1219,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Monira Akter",
"family_name": "Mou",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0009-0005-3456-4140",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 260
},
{
"id": 1220,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Forhad Ahmed",
"family_name": "Hridoy",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0009-0009-3994-3130",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 260
},
{
"id": 1221,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sadia Afrin",
"family_name": "Siddiqua",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0009-0006-7126-9762",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 260
},
{
"id": 1222,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Abul Kashem Fazlul Haque",
"family_name": "Bhuiyan",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-1917-6386",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 260
},
{
"id": 1223,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mohammad Shamsul Alam",
"family_name": "Bhuiyan",
"email": "msabhuiyan.abg@bau.edu.bd",
"author_order": 6,
"ORCID": "http://orcid.org/0000-0003-1525-2182",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Shamsul Alam Bhuiyan, PhD;\r\nDepartment of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Email: msabhuiyan.abg@bau.edu.bd",
"article": 260
}
]
},
{
"id": 259,
"slug": "178-1690727910-role-of-rs2762934-and-rs4809957-polymorphisms-of-cyp24a1-on-outcomes-of-vitamin-d-treatment-in-postmenopausal-osteoporotic-women-in-iraq",
"type": "original_article",
"manuscript_id": "178-1690727910",
"published": "2023-10-28",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/59/178-1690727910.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 65-73",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Abdulrahman NM, Ali SH. Role of rs2762934 and rs4809957 polymorphisms of CYP24A1 on outcomes of vitamin D treatment in postmenopausal osteoporotic women in Iraq. J Adv Biotechnol Exp Ther. 2024; 7(1): 65-73.",
"title": "Role of rs2762934 and rs4809957 polymorphisms of CYP24A1 on outcomes of vitamin D treatment in postmenopausal osteoporotic women in Iraq",
"abstract": "<p>Osteoporosis is widely distributed condition characterized by bone weakness making it more fragile and easier to be fractured. Various elements enter in bone structure making it rigid to tolerate body weight and any accident. Many factors had a role in progression of osteoporosis such as age, gender, medications, and genetic variations in enzymes responsible for vitamin D metabolism. The aim of the current study is to evaluate role of polymorphism of metabolic enzyme CYP24A1 on responding to vitamin D in postmenopausal osteoporotic women. Forty postmenopausal women were followed up for 2 months and took 50000IU/week of vitamin D during study period, and 30 postmenopausal women without osteoporosis as a control group. Blood samples were taken pre- and post-treatment to determine responder to vitamin D therapy. Regarding vitamin D level, there was a significant difference between responder and non-responder at pre-treatment levels. GG genotype of rs2762934 SNP and AA genotype of rs4809957 SNP are the predominant in non-responder group, and both genotypes showed negative correlation to being responsiveness to vitamin D therapy. The homozygote wild GG genotype of rs2762934 predict vitamin D non-responsiveness in sample of osteoporotic postmenopausal women. The AA genotype of the rs2762934 substantially increases the responsiveness while AA of rs4809957 is associated with non-responsiveness. In conclusion, this study will implicate in correct choosing the desired dose of vitamin D for desired osteoporotic women to get better response and avoid any harmful effect of vitamin D overdose.</p>",
"DOI": "10.5455/jabet.2024.d06",
"views": 914,
"downloads": 106,
"figures": [],
"authors": [
{
"id": 1216,
"affiliation": [
{
"affiliation": "Department of Clinical Pharmacy, College of Pharmacy, University of Baghdad, Baghdad, Iraq"
}
],
"first_name": "Noor Mohammed",
"family_name": "Abdulrahman",
"email": "nour.mohammed1100e@copharm.uobaghdad.edu.iq",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-0208-9227",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Noor Mohammed Abdulrahman, Department of Clinical Pharmacy, College of Pharmacy, University of Baghdad, Baghdad, Iraq. Email:nour.mohammed1100e@copharm.uobaghdad.edu.iq",
"article": 259
},
{
"id": 1217,
"affiliation": [
{
"affiliation": "Department of Clinical Laboratory Sciences, College of Pharmacy, University of Baghdad, Baghdad, Iraq"
}
],
"first_name": "Shatha Hussein",
"family_name": "Ali",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 259
}
]
},
{
"id": 258,
"slug": "178-1696060914-beneficial-effects-of-the-multi-strain-probiotic-preparation-bacimix-on-antibiotic-associated-diarrhea-in-rats",
"type": "original_article",
"manuscript_id": "178-1696060914",
"published": "2023-10-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/57/178-1696060914.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 53-64",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Nguyen HD, Nguyen ST, et al. Beneficial effects of the multi-strain probiotic preparation BaciMix on antibiotic-associated diarrhea in rats. J Adv Biotechnol Exp Ther. 2024; 7(1): 53-64.",
"title": "Beneficial effects of the multi-strain probiotic preparation BaciMix on antibiotic-associated diarrhea in rats",
"abstract": "<p>Dysbiosis is a common status of intestinal microbiota in modern society and is associated with many diseases. Diarrhea is a kind of dysbiosis and is frequently caused by imbalanced gut microbiota due to misuse of antibiotics. A lot of evidence has shown that probiotics can exhibit potential to alleviate the effects of antibiotic-associated diarrhea (AAD). In this study, Lincomycin was utilized to induce AAD in rats and then the effects of the multi-strain probiotic preparation BaciMix on this model were evaluated. The rat groups, including healthy control rats, AAD-induced rats, AAD rats with no treatment (self-healing rats), and AAD rats treated by BaciMix preparation, were analyzed regarding general health status, some immune indices, and intestinal microbiota changes. The results disclosed that the BaciMix preparation remarkably reduced the effects of the antibiotic regarding the diarrhea score and cecum thickness in the rats treated by BaciMix preparation. Additionally, the BaciMix preparation reduced pre-inflammatory cytokines, including TNF-a, and IL-6, while increasing the IgA in sera and intestinal mucosae. Moreover, the BaciMix preparation amended the compositions and differential abundance of intestinal bacteria of the rats to increase some beneficial bacteria like <em>Lactobacillus</em>, <em>Bacillus</em>, <em>Hungatella</em>, and <em>Romboutsia</em>, and to decrease some potentially harmful genera such as <em>Bacteroides</em>, <em>Escherichia-Shigella</em>, and <em>Proteus</em>. Generally, BaciMix preparation displayed helpful effects on the AAD rat model.</p>",
"DOI": "10.5455/jabet.2024.d05",
"views": 1358,
"downloads": 140,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/45/28/178-1696060914-Figure_1.jpg",
"caption": "Figure 1. Diarrhea score of the rats. TCG, control group; AAD, antibiotic-associated diarrhea group; THG, self-healing group; BAG, BaciMix group. The Mean SD is used to represent the values.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/45/28/178-1696060914-Figure_2.jpg",
"caption": "Figure 2. Microscopic image of the rat ceca. TCG group (A); AAD group (B); THG group (C); BAG group (D).",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/45/28/178-1696060914-Figure_3.jpg",
"caption": "Figure 3. Levels of IL-6 (A); TNF-α (B), IgA in serum (C), and IgA in intestinal mucosae (D) of rats. TCG, control group; AAD, antibiotic-associated diarrhea group; THG, self-healing group; BAG, BaciMix group. The Min - Max is used to represent the values, (*) p<0.05 compared with TCG, (#) p<0.05 compared with BAG.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/45/28/178-1696060914-Figure_4.jpg",
"caption": "Figure 4. The Simpson (A), Shannon (B), Evenness indices (C), and Chao 1 (D) of the intestinal bacteria in the 4 rat groups. TCG, control group; AAD, antibiotic-associated diarrhea group; THG, self-healing group; BAG, BaciMix group. The Mean ± SD is used to represent the values, (*) p<0.05 compared with TCG, (#) p<0.05 compared with AAD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/45/28/178-1696060914-Figure_5.jpg",
"caption": "Figure 5. The PCoA graph of the intestinal bacteria in the 4 rat groups. TCG, control group; AAD, antibiotic-associated diarrhea group; THG, self-healing group; BAG, BaciMix group.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/45/28/178-1696060914-Figure_6.jpg",
"caption": "Figure 6. The intestinal microbiota composition at phylum level of Bacteroidota (A), Firmicutes (B), Proteobacteria (C) of the 4 rat groups. TCG, control group; AAD, antibiotic-associated diarrhea group; THG, self-healing group; BAG, BaciMix group. The Min - Max is used to represent values. (*) p<0,05 compared with TCG, (#) p<0.05 compared with AAD, ($) p<0.05 compared with THG.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/45/28/178-1696060914-Figure_7.jpg",
"caption": "Figure 7. The genus compositions of Bacteroides (A), Proteus (B) Escherichia Shigella (C) of the four rat groups. TCG, control group; AAD, antibiotic-associated diarrhea group; THG, self-healing group; BAG, BaciMix group. The Min - Max is used to represent values. (*) p<0,05 compared with TCG, (#) p<0.05 compared with AAD, ($) p<0.05 compared with THG.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/45/28/178-1696060914-Figure_8.jpg",
"caption": "Figure 8. The genus compositions of Lactobacillus (A), Bacillus (B) Hungatella (C), Romboutsia (D) of the 4 rat groups. TCG, control group; AAD, antibiotic-associated diarrhea group; THG, self-healing group; BAG, BaciMix group. The Min - Max is used to represent values. (*) p<0,05 compared with TCG, (#) p<0.05 compared with AAD, ($) p<0.05 compared with THG.",
"featured": false
}
],
"authors": [
{
"id": 1210,
"affiliation": [
{
"affiliation": "Vietnam Military Medical University, Hanoi, Vietnam"
}
],
"first_name": "Duy Ha",
"family_name": "Nguyen",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 258
},
{
"id": 1211,
"affiliation": [
{
"affiliation": "Vietnam Military Medical University, Hanoi, Vietnam"
}
],
"first_name": "Thai Son",
"family_name": "Nguyen",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 258
},
{
"id": 1212,
"affiliation": [
{
"affiliation": "Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Dinh Toi",
"family_name": "Chu",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-4596-2022",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 258
},
{
"id": 1213,
"affiliation": [
{
"affiliation": "Vietnam Military Medical University, Hanoi, Vietnam"
}
],
"first_name": "Thi Hong Hanh",
"family_name": "Le",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 258
},
{
"id": 1214,
"affiliation": [
{
"affiliation": "VNU, Institute of Microbiology and Biotechnology, Hanoi, Vietnam"
}
],
"first_name": "Quynh Uyen",
"family_name": "Nguyen",
"email": "uyennq@vnu.edu.vn",
"author_order": 5,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Quynh Uyen Nguyen, VNU, Institute of Microbiology and Biotechnology, Hanoi, Vietnam, Email: uyennq@vnu.edu.vn",
"article": 258
},
{
"id": 1215,
"affiliation": [
{
"affiliation": "VNU, Institute of Microbiology and Biotechnology, Hanoi, Vietnam"
}
],
"first_name": "Hoang Van",
"family_name": "Vinh",
"email": "vinhhv@vnu.edu.vn",
"author_order": 6,
"ORCID": "https://orcid.org/0000-0001-7061-0515",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Hoang Van Vinh, VNU, Institute of Microbiology and Biotechnology, Hanoi, Vietnam, Email: vinhhv@vnu.edu.vn",
"article": 258
}
]
},
{
"id": 257,
"slug": "178-1691034882-human-mesenchymal-stem-cell-secretome-lowers-caspase-3-levels-and-apoptosis-in-hepatocytes-of-cholestatic-rats",
"type": "original_article",
"manuscript_id": "178-1691034882",
"published": "2023-10-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/28/178-1691034882.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 44-52",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Purwanto NF, Susilaningsih N. et al. Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic rats. J Adv Biotechnol Exp Ther. 2024; 7(1): 44-52.",
"title": "Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic rats",
"abstract": "<p>Liver cirrhosis is a major cause of morbidity and mortality in the world. Definitive therapy is liver transplantation, but it is constrained by difficulties in finding a transplant donor. Human mesenchymal stem cell-based therapy can help liver regeneration directly, through hepatogenic differentiation, or indirectly through the paracrine secretome. Thus, this study aims to determine the effect of Human mesenchymal stem cell secretome (HuMSC-S) administration on caspase 3 levels and apoptotic hepatic cells in a rat model with cholestasis after choledochal duct ligation receiving urso deoxy cholic acid (UDCA). Twenty-four male Wistar rats were subjected to a choledochal duct ligation and were included in this randomized experimental study. After surgical intervention, all rats were randomly assigned into 4 group: control, UDCA, HuMSC-S, and a combination of UDCA and HuMSC-S for 4 weeks. Caspase-3 levels were assessed from the blood sample, while the apoptosis of hepatocytes was evaluated using histopathologic examination of the liver. Interestingly, caspase-3 level was significantly lower in the UDCA and HuMSC-S-treated groups compared to the UDCA or HuMSC-S alone. Similarly, the apoptosis of hepatocyte was significantly lower in the combination group compared to the UDCA or HuMSC-S alone. In conclusion, addition of HuMSC-S to UDCA lowered caspase-3 levels and apoptotic cell count in rats with hepatic cholestasis after choledochal duct ligation.</p>",
"DOI": "10.5455/jabet.2024.d04",
"views": 780,
"downloads": 162,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/52/25/178-1691034882-Figure_1.jpg",
"caption": "Figure 1. The boxplot graphic of caspase 3 levels in each group. There was a statistically significant difference (One Way ANOVA, p<0.05), and there was also a significant difference when compared in each group (Post Hoc test); n=6, *significant. Control (K1), UDCA therapy (K2), HuMSC-S therapy (K3), and combination of UDCA and HuMSC-S (K4).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/25/178-1691034882-Figure_2.jpg",
"caption": "Figure 2. Histopathology of apoptotic hepatocyte. Control (K1), UDCA therapy (K2), HuMSC-S therapy (K3), and combination of UDCA and HuMSC-S (K4). There were 6 samples per group. Apoptosis of hepatocyte marked with black arrow, while normal hepatocyte marked with blue arrow. Scale bar: 800x448 pixels.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/25/178-1691034882-Figure_3.jpg",
"caption": "Figure 3. The boxplot graphic of apoptosis of hepatocyte in each group. There was a statistically significant difference (One Way ANOVA p<0.05), and there was also a significant difference when compared in each group (Post Hoc test); n=6, *significant. Control (K1), UDCA therapy (K2), HuMSC-S therapy (K3), and combination of UDCA and HuMSC-S (K4).",
"featured": false
}
],
"authors": [
{
"id": 1205,
"affiliation": [
{
"affiliation": "Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Naufal Faruq",
"family_name": "Purwanto",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0000-3035-2291",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 257
},
{
"id": 1206,
"affiliation": [
{
"affiliation": "Department of Anatomy Histology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Neni",
"family_name": "Susilaningsih",
"email": "nsusilaningsih@gmail.com",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-1960-507X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Neni Susilaningsih, Department of Anatomy Histology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia. Email: nsusilaningsih@gmail.com",
"article": 257
},
{
"id": 1207,
"affiliation": [
{
"affiliation": "Department of Digestive Surgery, Dr Kariadi General Hospital / Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Bernardus Parish",
"family_name": "Budiono",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-4704-3481",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 257
},
{
"id": 1208,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutic, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Nani",
"family_name": "Maharani",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0001-5984-6439",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 257
},
{
"id": 1209,
"affiliation": [
{
"affiliation": "Department of Digestive Surgery, Dr Kariadi General Hospital / Faculty of Medicine, Diponegoro University, Semarang, Indonesia"
}
],
"first_name": "Ignatius",
"family_name": "Riwanto",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0003-3441-3738",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 257
}
]
},
{
"id": 255,
"slug": "178-1691326414-effectiveness-of-a-gluten-free-diet-on-the-autoantibody-frequency-of-endocrine-and-neuron-disorders-in-patients-with-celiac-disease",
"type": "original_article",
"manuscript_id": "178-1691326414",
"published": "2023-10-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/40/178-1691326414.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 34-43",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Elia ZN, Berwary NJA. Effectiveness of a gluten-free diet on the autoantibody frequency of endocrine and neuron disorders in patients with celiac disease. J Adv Biotechnol Exp Ther. 2024; 7(1): 34-43",
"title": "Effectiveness of a gluten-free diet on the autoantibody frequency of endocrine and neuron disorders in patients with celiac disease",
"abstract": "<p>Several possible associations between different types of autoimmune diseases have been reported, therefore this study aimed to investigate the relationship between celiac disease (CD) and some related autoimmune diseases by determining the effect of gluten on severity of these diseases. Two hundred blood samples were collected, 50 samples were withdrawn from healthy donors as a control group, 75 were collected from newly diagnosed (ND) CD patients, while the remaining blood samples (75) were obtained from the same patients after 4 months of gluten free diet (GFD) regime. Sera were separated to measure the level of anti-tissue transglutaminase IgA (tTg-IgA), anti-pituitary IgG, anti- thyroid peroxidase IgG (TPO- IgG), anti-islet IgG and anti-ganglioside IgG (IU/ml) by enzyme linked immunosorbent assay (ELISA). The results recorded high significant elevation (p>0.0001) in the concentrations of all autoantibodies of both ND and GFD groups compared with their concentrations in the control group. Furthermore, after 4 months of GFD, concentrations of all autoantibodies revealed highly significant decreases (p>0.0001) as compared with ND. Regarding the correlation of all studied autoantibodies with anti tTG-IgA, only anti-ganglioside IgG levels were weakly correlated in pre-GFD treatment patients, meanwhile anti-ganglioside IgG and anti-pituitary IgG were also recorded weak correlations in post- GFD of CD patients. This study concluded that GFD helps to reduce the severity of endocrine and neuron autoimmune disorders in addition to CD, which have been proved by decreasing in the level of their diagnostic autoantibodies.</p>",
"DOI": "10.5455/jabet.2024.d03",
"views": 1222,
"downloads": 130,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/16/19/178-1691326414-Figure1.jpg",
"caption": "Figure 1. Multiple correlation (r value) of association between different auto-immune antibodies among ND (Newly Diagnosed) group.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/16/19/178-1691326414-Figure2.jpg",
"caption": "Figure 2. Multiple correlation (r value) of association between different auto-immune antibodies among GFD (Gluten Free Diet) group.",
"featured": false
}
],
"authors": [
{
"id": 1201,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Zaid Nabeel",
"family_name": "Elia",
"email": "m.zaid@epu.edu.iq",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-4993-1159",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Zaid Nabeel Elia; Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq. Email: m.zaid@epu.edu.iq",
"article": 255
},
{
"id": 1202,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Najat Jabbar Ahmed",
"family_name": "Berwary",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 255
}
]
},
{
"id": 254,
"slug": "178-1693333296-impact-of-single-nucleotide-polymorphisms-of-immune-checkpoint-ctla-4-snp-rs231775-and-rs5742909-in-susceptibility-to-hashimotos-thyroiditis-patients",
"type": "original_article",
"manuscript_id": "178-1693333296",
"published": "2023-10-17",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/47/178-1693333296.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 7(1): 23-33",
"academic_editor": "Mohammad Nazrul Islam, PhD; Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh",
"cite_info": "Mahmood AM, Allami RH, et al. Impact of single nucleotide polymorphisms of immune checkpoint CTLA-4 (SNP rs231775 and rs5742909) in susceptibility to Hashimoto's thyroiditis patients. J Adv Biotechnol Exp Ther. 2023; 7(1): 23-33",
"title": "Impact of single nucleotide polymorphisms of immune checkpoint CTLA-4 (SNP rs231775 and rs5742909) in susceptibility to Hashimoto's thyroiditis patients",
"abstract": "<p>Hashimoto thyroiditis (HT) is an autoimmune disorder causing thyroid cell destruction. This research aimed to evaluate CTLA-4 expression levels in patients with HT. As well as understand the risk and protective effects of two CTLA-4 SNPs polymorphisms (rs231775 and rs5742909) in Iraqi patients. A case control study was conducted on 50 HT patients (5 men and 45 females) which were assembled from Baghdad teaching hospital, Baghdad, Iraq. The relative gene expression method was used to measure the gene expression of CTLA-4 using qRT-PCR. The single nucleotide polymorphisms of (318C/T (rs231775) and +49A/G (rs5742909) were sequenced for data analysis. There was a significant increase of serum CTLA-4 levels and, there was a significant increase in fold change of CTLA-4 in HT patients compared to controls. The allele and genotype frequencies according to Hardy Weinberg equilibrium showed significant differences in HT patients in the study, the 318C/T (rs231775) variant showed differences in the AA genotype when compared to controls. However, there were no significant differences observed in the AG and GG genotypes. Similarly, for the CTLA-4 SNP +49A/G (rs5742909), no significant differences were found in the CC, CT, and TT genotypes. Despite these observations, both studied SNPs showed an increased expression of the soluble form and fold change of the immune checkpoint CTLA-4 in HT patients. The study reveals that CTLA-4 gene SNPs rs231775 and rs5742909 significantly influence the expression of CTLA-4 levels in HT patients, which potentially may cause HT incidence and development.</p>",
"DOI": "10.5455/jabet.2024.d02",
"views": 1488,
"downloads": 96,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/31/17/Figure_1.jpg",
"caption": "Figure 1. Serum CTLA-4 levels. Mean ± SE according to rs231775 genotype frequency in patients.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/31/17/Figure_2.jpg",
"caption": "Figure 2. Gene expression of CTLA-4 levels. Mean ± SE according to rs231775 genotype frequency in patients.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/31/17/Figure_3.jpg",
"caption": "Figure 3. Serum CTLA-4 levels. Mean ± SE according to rs5742909 SNP in patients.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/31/17/Figure_4.jpg",
"caption": "Figure 4. Gene expression of CTLA-4 levels. Mean ± SE according to rs5742909SNP in patients.",
"featured": false
}
],
"authors": [
{
"id": 1198,
"affiliation": [
{
"affiliation": "College of Biotechnology, Al-Nahrain University, Baghdad, Iraq"
},
{
"affiliation": "Chest and Respiratory Diseases Unit, Kadhimiya, Baghdad, Iraq"
}
],
"first_name": "Asalh M.",
"family_name": "Mahmood",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0009-0009-0911-8383",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 254
},
{
"id": 1199,
"affiliation": [
{
"affiliation": "College of Biotechnology, Al-Nahrain University, Baghdad, Iraq"
}
],
"first_name": "Risala H.",
"family_name": "Allami",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-3000-4614",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 254
},
{
"id": 1200,
"affiliation": [
{
"affiliation": "Department of Anesthesia Techniques, Madenat Alelem University College, Baghdad, Iraq"
}
],
"first_name": "Yasir W.",
"family_name": "Issa",
"email": "yasirw.issa@mauc.edu.iq",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0001-5427-0602",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Yasir W. Issa \r\nDepartment of Anesthesia Techniques, Madenat Alelem University College, Baghdad, Iraq. Email: yasirw.issa@mauc.edu.iq",
"article": 254
}
]
},
{
"id": 253,
"slug": "178-1691853907-unveiling-the-immunomodulatory-mechanisms-of-pineapple-metabolites-a-multi-modal-computational-analysis-using-network-pharmacology-molecular-docking-and-molecular-dynamics-simulation",
"type": "original_article",
"manuscript_id": "178-1691853907",
"published": "2023-10-04",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/06/178-1691853907.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2024; 7(1): 01-22",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Tallei TE, Fatimawali F, et al. Unveiling the immunomodulatory mechanisms of pineapple metabolites: A multi-modal computational analysis using network pharmacology, molecular docking, and molecular dynamics simulation. J Adv Biotechnol Exp Ther. 2024; 7(1): 01-22.",
"title": "Unveiling the immunomodulatory mechanisms of pineapple metabolites: A multi-modal computational analysis using network pharmacology, molecular docking, and molecular dynamics simulation",
"abstract": "<p>The exploration of the immunomodulatory potential of pineapple metabolites holds promise for substantial implications across several fields, encompassing medicine, pharmacology, nutrition, and public health. This study explores potential immune-regulating properties of secondary pineapple metabolites beyond bromelain, using computational techniques. Pineapple juice's secondary metabolites were identified via LC-MS-based metabolomics, selected using KNApSAcK Kanaya and Dr. Duke's databases. A heatmap was generated with Orange v.3.27.0. Bioactivity predictions utilized the PASS Online webserver, while ADMET properties were forecasted. Network pharmacology explored metabolite interactions with protein targets. Molecular docking focused on compounds against receptors, choosing robust interactors for dynamic simulations. Our findings revealed that certain metabolites from pineapple juice/fermented juice exhibited interactions with proteins associated with pro-inflammatory cytokines. Specifically, the molecular docking results indicated that the carbohydrate moiety of bromelain (CMB) interacted strongly with TLR2, while 9,10-Dihydroxystearic acid showed strong interactions with NLRP3 (inflammasome). The flexibility and stability of these complexes were further assessed through molecular dynamics simulations conducted over a 50-ns time period. The MM-PBSA calculations also indicated low binding free energies between these complexes, suggesting strong molecular interactions. These findings suggest that CMB may interact with TLR2 and 9,10-Dihydroxystearic acid may interact with NLRP3, highlighting their potential as immunomodulatory agents. However, further experimental studies are warranted to confirm the therapeutic efficacy of these molecules and investigate their mechanisms of action in vivo.</p>",
"DOI": "10.5455/jabet.2024.d01",
"views": 1595,
"downloads": 179,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/04/178-1691853907-figure1.jpg",
"caption": "Figure 1. An illustrative summary of the methodologies used to investigate the immunomodulatory effects of metabolites in both raw and fermented pineapple juice.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/04/178-1691853907-figure2.jpg",
"caption": "Figure 2. Heatmap of 12 compounds in pineapple juice (JN) and fermented pineapple juice (F- JN) from the second stage of selection.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/04/178-1691853907-figure3.jpg",
"caption": "Figure 3. Heatmap created from compounds selected in the second stage of the selection process based on their relative abundance values.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/04/178-1691853907-figure4.jpg",
"caption": "Figure 4. Potential of bioactive compounds as immunomodulators based on the Pa values obtained through PASS Online webserver.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/04/178-1691853907-figure5.jpg",
"caption": "Figure 5. Results of PPI (Protein-Protein Interaction) analysis, along with associated activities in biological processes, visualized using Cytoscape.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/04/178-1691853907-figure6.jpg",
"caption": "Figure 6. Glide XP molecular docking results of 89377 with NACHT, LRR and PYD domains-containing protein 3 (NLRP3) and 44263865 with toll-like receptor 2 (TLR2). The binding modes of (A) 89377-NLRP3 and (B) 44263865-TLR2. The schematic 2D interactions of (C) 89377-NLRP3 and (D) 44263865-TLR2.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/04/178-1691853907-figure7.jpg",
"caption": "Figure 7. Trajectory analysis of molecular dynamics simulation of toll-like receptor 2 (TLR2) and the carbohydrate moiety of bromelain complex. (A) RMSD, (B) RMS fluctuation, (C) Rg, and (D) SAS area values of TLR2-44263865 complex and apoprotein during the period of 50 ns simulation.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/04/178-1691853907-figure8.jpg",
"caption": "Figure 8. Molecular dynamics simulation of 89377 with NACHT, LRR and PYD domains-containing protein 3 (NLRP3). (A) RMSD, (B) RMS fluctuation, (C) Rg, and (D) SAS area values of NLRP3-89377 complex and apoprotein during the period of 50 ns simulation.",
"featured": false
}
],
"authors": [
{
"id": 1190,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Trina Ekawati",
"family_name": "Tallei",
"email": "trina_tallei@unsrat.ac.id",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-7963-7527",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Trina Ekawati Tallei, PhD; Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia. Email: trina_tallei@unsrat.ac.id",
"article": 253
},
{
"id": 1191,
"affiliation": [
{
"affiliation": "Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Fatima",
"family_name": "wali",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 253
},
{
"id": 1192,
"affiliation": [
{
"affiliation": "Department of Animal Production, Faculty of Animal Husbandry, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Afriza",
"family_name": "Yelnetty",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 253
},
{
"id": 1193,
"affiliation": [
{
"affiliation": "Department of Dermatology and Venereology, Faculty of Medicine, University of Sam Ratulangi, Manado, 95163, North Sulawesi, Indonesia"
}
],
"first_name": "Nurdjannah Jane",
"family_name": "Niode",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 253
},
{
"id": 1194,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences Education, Universitas Pendidikan Indonesia, Bandung 40154, Indonesia"
}
],
"first_name": "Diah",
"family_name": "Kusumawaty",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 253
},
{
"id": 1195,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia"
}
],
"first_name": "Rinaldi",
"family_name": "Idroes",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 253
},
{
"id": 1196,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Erciyes University, Kayseri 38039, Turkey"
}
],
"first_name": "Ismail",
"family_name": "Celik",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 253
},
{
"id": 1197,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh"
},
{
"affiliation": "Department of Pharmacy, Faculty of Allied Health Sciences, Daffodil International University, Dhaka 1207, Bangladesh"
}
],
"first_name": "Talha Bin",
"family_name": "Emran",
"email": "talhabmb@bgctub.ac.bd",
"author_order": 8,
"ORCID": "https://orcid.org/0000-0003-3188-2272",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran, PhD; Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh. \r\nEmail: talhabmb@bgctub.ac.bd",
"article": 253
}
]
}
]
}
],
"title": "2024",
"slug": "2024",
"order": 7
},
{
"id": 6,
"issues": [
{
"id": 20,
"title": "Vol6 Issue3",
"cover": "https://jabet.bsmiab.org/media/issue_cover/Jabet_Coverpage_W5Y84Hg.jpg",
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2023",
"slug": "2023-vol6-issue3",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 256,
"slug": "178-1682259243-spanocimum-basilicumspan-extract-modulates-tau-aggregation-and-improves-memory-function-in-a-neurodegenerative-rat-model",
"type": "original_article",
"manuscript_id": "178-1682259243",
"published": "2023-09-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/16/178-1682259243.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 728-738",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, \r\nDhaka-1230, Bangladesh",
"cite_info": "Shalan MA, Alhasan L. <span>Ocimum basilicum</span> extract modulates Tau aggregation and improves memory function in a neurodegenerative rat model. J Adv Biotechnol Exp Ther. 2023; 6(3): 728-738",
"title": "<span>Ocimum basilicum</span> extract modulates Tau aggregation and improves memory function in a neurodegenerative rat model",
"abstract": "<p>Alzheimer disease (AD) is a neurological condition that worsens with time. <em>Ocimum basilicum</em> is a well-known plant that has been used for centuries in many different cultures throughout the world as a treatment for lung disorders, anti-diarrhea medication, painkiller for the abdomen, and anti-inflammatory and antioxidant. The main objective of this study was evaluation of the neuroprotective potential effect of <em>Ocimum basilicum</em> on oxidative stress status in rat induced AD. Fifty rats were divided into five groups (10 rats/group) such as i) Control, ii) AlCl3-induced AD, iii) AlCl3 rats treated with rivastigmine (3 mg/kg/day), iv) AlCl3 rats treated with <em>Ocimum basilicum</em> (250 mg/kg/day), and v) AlCl3 rats treated with <em>Ocimum basilicum</em> (500 mg/kg/day). The results showed that the <em>Ocimum basilicum</em> plant leaves extract increased serum superoxide dismutase with a significant decrease of a serum MDA while the aggregation of tau protein expression was decreased. Histological changes were observed in brain tissues of AD rats. However, the high dosing of the plant leaves extract (500 mg/kg) was more powerful than the low treatment with low dose (250 mg/kg) by decreasing tau protein expression. The results suggest that <em>Ocimum basilicum</em> can relieve symptoms and prevent the progression of AD severity by improving memory function. It can be concluded that <em>Ocimum basilicum</em> leaves alleviated the memory impairment and learning abilities due to antioxidants activity of flavonoids, tannins and terpenoids.</p>",
"DOI": "10.5455/jabet.2023.d162",
"views": 1466,
"downloads": 161,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/52/19/178-1682259243-Figure_1.jpg",
"caption": "Figure 1. Natural view of Ocimum basilicum leaves.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/52/19/178-1682259243-Figure_2.jpg",
"caption": "Figure 2. Effect Ocimum basilicum extract on antioxidant activity in AlCl3-induced AD rats (n=10 for each group). Antioxidant activity was screened SOD assay kit. The mean ± SD of SOD in U/ml in all studied groups of rats. * Symbols signify the levels of significance at p < 0.001 across groups using one way ANOVA.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/52/19/178-1682259243-Figure_3.jpg",
"caption": "Figure 3. Effect Ocimum basilicum extract on oxidative stress status in AlCl3-induced AD rats (n=10 each group). Oxidative stress status was screened MDA assay kit. The mean ± SD of MDA in nmol/ml in all studied groups of rats. * Symbols signify the levels of significance at p < 0.001 across groups using one way ANOVA.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/52/19/178-1682259243-Figure_4.jpg",
"caption": "Figure 4. Expression of tau protein in brain section. Photomicrograph of hippocampus of control, AD, rivastigmine, Ocimum basilicum 250 mg/kg and 500mg/kg-treated group. (A) Showing normal histological structure of hippocamus. (B) Photomicrograph of brain section of AD-induced rats displaying tau protein expression was observed irregularly (black arrow) inside concavity of Cornu Ammonis layer of hippocampus. (C) Photomicrograph showing normal structure of hippocampus with neurofibrillary tangles (NFTs) (black arrow) morphology was observed in one neuron of molecular layer. (D) Photomicrograph displaying, positive expression of tau protein was observed in Dentate gyrus (black arrow) layer of hippocampus. (E) Photomicrograph of healthy hippocampus shows positive expression of Tau protein (black arrow) in Cornu Ammonis, where the expression of tau protein was observed surrounded nucleus of neurons. DAB and Hematoxylin 400x.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/52/19/178-1682259243-Figure_5.jpg",
"caption": "Figure 5. Effect Ocimum basilicum extract on tau protein expression in in AlCl3-induced AD rats (n=10 each group). The mean ± SD of tau protein expression in all studied groups of rats. *Symbols signify the levels of significance at p < 0.001 across groups using one way ANOVA.",
"featured": false
}
],
"authors": [
{
"id": 1203,
"affiliation": [
{
"affiliation": "Department of Biology, College of Education for Pure Science, University of Thi-Qar, Nasiriyah, Iraq"
}
],
"first_name": "Marwa Amer Shalan",
"family_name": null,
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 256
},
{
"id": 1204,
"affiliation": [
{
"affiliation": "Department of Biology, College of Education for Pure Science, University of Thi-Qar, Nasiriyah, Iraq"
}
],
"first_name": "Layla Alhasan",
"family_name": null,
"email": "layla.alhassan14@gmail.com",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-9106-4426",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Layla Alhasan; Biology Department, Education College for Pure Sciences, University of Thi-Qar, Nasiriyah, Iraq. Email: layla.alhassan14@gmail.com",
"article": 256
}
]
},
{
"id": 252,
"slug": "178-1693302737-artificial-intelligence-a-powerful-tool-to-combat-antimicrobial-resistance-an-update",
"type": "review_article",
"manuscript_id": "178-1693302737",
"published": "2023-09-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/37/178-1693302737.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 711-727",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Masud RI, Fahim NAI, et al. Artificial intelligence, a powerful tool to combat antimicrobial resistance: An update. J Adv Biotechnol Exp Ther. 2023; 6(3): 711-727",
"title": "Artificial intelligence, a powerful tool to combat antimicrobial resistance: An update",
"abstract": "<p>Antimicrobial resistance (AMR), also known as antibiotic resistance, is a global health crisis. Many people die from diseases caused by multi-drug resistant (MDR) pathogens. Artificial intelligence (AI) involves creating computer systems that can perform tasks typically requiring human intelligence, such as visual perception, speech recognition, decision-making, and language translation. AI has been successfully used in various fields, from single-cell study to space science. There is huge potential for using AI in medical science, including fighting against MDR to combat AMR. In this review, we have highlighted some of AI’s successful uses and potentiality to combat AMR for the betterment of the human race, including the basic principles of AI, available AI resources, use and scope, advantages and limitations. In addition, in this review, we also have highlighted new research areas such as AMR detection, new drug development etc. to control of AMR.</p>",
"DOI": "10.5455/jabet.2023.d161",
"views": 4150,
"downloads": 333,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/29/178-1693302737-Figure1.jpg",
"caption": "Figure 1. The usage of AI in various health sectors to speed up proper and accurate diagnosis and treatment procedures.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/29/178-1693302737-Figure2.jpg",
"caption": "Table 1. Current accessible image collection, utilized in deep learning experiments to identify various microorganisms, aids in precise disease diagnosis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/29/178-1693302737-Figure3.jpg",
"caption": "Figure 2. Effect of AI-discovered beta-β-lactamase inhibitors combined with antibiotics on β-lactamase-containing bacteria.",
"featured": false
}
],
"authors": [
{
"id": 1185,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Rony Ibne",
"family_name": "Masud",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 252
},
{
"id": 1186,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Naeem Ahammed Ibrahim",
"family_name": "Fahim",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 252
},
{
"id": 1187,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Liton",
"family_name": "Rana",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 252
},
{
"id": 1188,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
},
{
"affiliation": "Department of Animal Sciences, University of California—Davis, Davis, CA 95616, USA"
}
],
"first_name": "Md. Saiful",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 252
},
{
"id": 1189,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Tanvir",
"family_name": "Rahman",
"email": "tanvirahman@bau.edu.bd",
"author_order": 5,
"ORCID": "https://orcid.org/0000-0001-5432-480X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Tanvir Rahman, PhD; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Email: tanvirahman@bau.edu.bd",
"article": 252
}
]
},
{
"id": 251,
"slug": "178-1690784589-next-generation-sequencing-for-the-formalin-fixed-paraffin-embedded-samples-of-ovarian-cancer-in-vietnam",
"type": "original_article",
"manuscript_id": "178-1690784589",
"published": "2023-09-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/07/178-1690784589.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 701-710",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Chu DT, Nguyen NQ, et al. Next generation sequencing for the formalin-fixed paraffin-embedded samples of ovarian cancer in Vietnam. J Adv Biotechnol Exp Ther. 2023; 6(3): 701-710",
"title": "Next generation sequencing for the formalin-fixed paraffin-embedded samples of ovarian cancer in Vietnam",
"abstract": "<p>Ovarian cancer is one of the most common causes of mortality among women, and the prevalence of ovarian cancer increases. Early diagnosis of this disease via genetic variant testing is one potential strategy for enhancing treatment and disease outcome. Our aim was to establish a standard procedure of next generation sequencing (NGS) for the formalin-fixed paraffin-embedded (FFPE) forms of ovarian tumor tissue to detect genetic mutation in our laboratory. Here, we used the FFPE samples of ovarian tumor tissues from Vietnamese patients to detect pathogenic variants in BRCA1/BRCA2 via the NGS. DNA was extracted using the QIAamp DNA FFPE Tissue Kit, and then its quality was assessed by the BioDrop and Qubit. The BRCAaccuTest<sup>TM</sup> PLUS kit and Illumina MiSeqDx instrument were used for both library preparation and sequencing. All samples had passed the A260/280 ratio cut-off for DNA purity and the requirement of DNA concentration. Excepted for the 1<sup>st</sup> time, the percentage of ≥ Q30 was more than 80%, while the density was approximately 1,200 K/mm2, while the phasing and prephasing (%) metrics were satisfied to be less than 0.1%. Five pathogenic variants in BRCA1/BRCA2, including both single nucleotide polymorphisms and indels were successfully detected using NgeneAnalySys<sup>TM</sup> software. In conclusion, DNA extraction from the FFPE sample was qualified for sequencing and the sequencing results met all the required metrics for variant analysis.</p>",
"DOI": "10.5455/jabet.2023.d160",
"views": 1316,
"downloads": 224,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/06/178-1690784589-Figure1.jpg",
"caption": "Figure 1. DNA quantification of sample OC46. A. By BioDrop UV-Visible spectrophotometer; B. By Invitrogen Qubit 4 Fluorometer. The quality of DNA extraction was controlled by both DNA concentration and A260/A280 ratio. These parameters were measured by both BioDrop UV-Visible spectrophotometer and Invitrogen Qubit 4 Flourometer. Results from this equipment were visualized in Figure 1. For example, DNA extracted from the OC46 patient had an A260/280 ratio of 1.919, while its concentration was 35.49 µL/mL (= 35.49 ng/µL) and 20.5 ng/µL according to the two equipment.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/06/178-1690784589-Figure2.jpg",
"caption": "Figure 2. The comparison of DNA qualification by BioDrop and Qubit. The DNA concentration of 33 samples measured by Qubit was statistically lower than DNA concentration measured by BioDrop with a p-value of 0.0001.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/06/178-1690784589-Figure3.jpg",
"caption": "Figure 3. Sample results of quality control of sequencing running using SAV (Read 3 of Run 3). A. Q score distribution; B. Data by Cycle; C. Q Score Heatmap. The Q Score distribution shows a quick overview of running quality. The QC30 for the whole run was 91.3%, and the estimated yield was 0.4 G. The Data by Cycle shows the intensity of different bases by color, including A, T, G C, for each cycle of the running. The Q score heatmap shows the Q score of each cycle. The Q score was lower at the first and middle cycles.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/06/178-1690784589-Figure4.jpg",
"caption": "Figure 4. Mapping statistics for samples. The workflow of raw read processing included the percentage of reads after each step. Of 432,151 paired-end raw reads, only 74.00% were primer reads. The percentage of reads that remained after trimming and quality control was 61.00%. After mapping the reference genome, the target and merged reads that were used for variant calling accounted for 60.00%.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/06/178-1690784589-Figure5.jpg",
"caption": "Figure 5. Example of c.928C>T variant in BRCA1 of OC48 sample. The sequence data of BRCA1 of both reference genome and OC48 patient at the position from 908 to 947, where involved the c.928C>T variant.",
"featured": false
}
],
"authors": [
{
"id": 1181,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
},
{
"affiliation": "Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Dinh-Toi",
"family_name": "Chu",
"email": "chudinhtoi.hnue@mail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-4596-2022",
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "Dinh-Toi Chu, PhD; Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam.\r\nE-mail: chudinhtoi.hnue@mail.com",
"article": 251
},
{
"id": 1182,
"affiliation": [
{
"affiliation": "Vietnam National Cancer Hospital, Hanoi, Vietnam"
}
],
"first_name": "Ngoc Quang",
"family_name": "Nguyen",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 251
},
{
"id": 1183,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
},
{
"affiliation": "Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Nhat-Le",
"family_name": "Bui",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 251
},
{
"id": 1184,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Thuy-Duong",
"family_name": "Vu",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 251
}
]
},
{
"id": 250,
"slug": "178-1689595300-phenotypic-screening-of-extended-spectrum-beta-lactamase-producing-salmonella-in-retail-shrimp",
"type": "original_article",
"manuscript_id": "178-1689595300",
"published": "2023-09-04",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/39/178-1689595300.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 686-700",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Afrin D, Ahmed M, et al. Phenotypic screening of extended-spectrum beta-lactamase producing Salmonella in retail shrimp. J Adv Biotechnol Exp Ther. 2023; 6(3): 686-700.",
"title": "Phenotypic screening of extended-spectrum beta-lactamase producing Salmonella in retail shrimp",
"abstract": "<p>The occurrence of extended-spectrum beta-lactamase (ESBL) producing bacteria is concerning the scientific community since it confers multiple drug resistance (MDR). The study investigated the prevalence of MDR-ESBL-producing <em>Salmonella</em> strains from shrimp in, Bangladesh.<strong> </strong>A total of 165 shrimp samples were processed from 55 shrimp specimens from different retail shops. The presence of <em>Salmonella</em> was confirmed by standard methods followed by antibiotic susceptibility testing. Isolates exhibiting resistance to third-generation cephalosporin were considered as <em>Salmonella</em> positive isolates which was later proven by the double disc synergy test. Out of the total of 39 isolates tested, 18 were found to be <em>Salmonella</em> positive and originated from 7 departmental stores. The remaining 21 positive isolates were obtained from the local market. The body had the highest rate of positive samples (30.91%), followed by the head (23.63%), and the tail (16.36%). Additionally, isolated <em>Salmonella</em> were resistant to rifampicin and cefixime but 100% susceptible to co-trimoxazole, ofloxacin, streptomycin, nalidixic acid, and chloramphenicol, while ciprofloxacin showed intermediate resistance. Among the drugs tested, vancomycin, cephalexin, ampicillin, and colistin exhibited extreme resistance. Finally, while 6 of the tested isolates demonstrated resistance against the recommended cephalosporin, three of them (7.69%) were <em>Salmonella</em> ESBL positive in the double disc synergy test. In conclusion, the rising incidence of MDR and the developing prevalence of ESBL-positive<em> Salmonella </em>may put a burden on the healthcare system by limiting access to effective antibacterial drugs. </p>",
"DOI": "10.5455/jabet.2023.d159",
"views": 1438,
"downloads": 229,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/06/178-1689595300-Figure1.jpg",
"caption": "Figure 1. Identification of Salmonella observing colony morphology. A) Glistering and opalescent colonies, grayish white in color on nutrient agar, B) Black centered colonies on SS agar, C) Colorless on MC agar, and D) Gram negative properties of Salmonella under microscope.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/06/178-1689595300-Figure2.jpg",
"caption": "Figure 2. Identification of Salmonella based on biochemical test whereas; A) Methyl Red, B) Voges-Proskauer, C) Citrate Utilization, D) Urease E) Catalase, F) Oxidase G) Motility, and H) Indole test.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/06/178-1689595300-Figure3.jpg",
"caption": "Figure 3. Distribution of Salmonella in three body parts (head, body, and tail) among the 39 positive samples.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/06/178-1689595300-Figure4.jpg",
"caption": "Figure 4. Some representative pictures (A, B, C, D, E and F) of the interpretation of the zone of inhibition for antimicrobial susceptibility status using the disc diffusion method.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/06/178-1689595300-Figure5.jpg",
"caption": "Figure 5. Percentage of frequency of each antibiotic sensitivity tested against isolated Salmonella. (Antibiotics that showed intermediate resistance were not considered as resistant).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/06/178-1689595300-Figure6.jpg",
"caption": "Figure 6. Prevalence of MDR Salmonella spp. according to drug groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/06/178-1689595300-Figure7.jpg",
"caption": "Figure 7. Some representative pictures (A, B, C, D, E and F) of initial ESBL screening of Salmonella by disc diffusion test employing five cephalosporin (cefixime, cephalexin, cefotaxime, ceftriaxone, and ceftazidime).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/18/06/178-1689595300-Figure8.jpg",
"caption": "Figure 8. Double disc synergy test for phenotypic confirmation of ESBL producing Salmonella using the AMC. a) Distortion in the indicator cephalosporin, and b) a clear enhancement in the zone of inhibition.",
"featured": false
}
],
"authors": [
{
"id": 1171,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Animal and Fish Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Dilruba",
"family_name": "Afrin",
"email": "afrin.afb@sau.ac.bd",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-7880-2092",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dilruba Afrin; Department of Animal and Fish Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh. E-mail: afrin.afb@sau.ac.bd",
"article": 250
},
{
"id": 1172,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Animal and Fish Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Milad",
"family_name": "Ahmed",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 250
},
{
"id": 1173,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Plant and Environmental Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Anik",
"family_name": "Banik",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 250
},
{
"id": 1174,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Animal and Fish Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Nazmul Islam",
"family_name": "Bappy",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 250
},
{
"id": 1175,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Plant and Environmental Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Md. Salman",
"family_name": "Sayem",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 250
},
{
"id": 1176,
"affiliation": [
{
"affiliation": "Department of Pathology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Masudur",
"family_name": "Rahman",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0001-7095-9303",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 250
},
{
"id": 1177,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Plant and Environmental Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Shamsunnahar",
"family_name": "Mukta",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 250
},
{
"id": 1178,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Animal and Fish Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Kazi Md. Ali",
"family_name": "Zinnah",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 250
},
{
"id": 1179,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Mohammad Mehedi Hasan",
"family_name": "Khan",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 250
},
{
"id": 1180,
"affiliation": [
{
"affiliation": "Department of Dairy Science, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Sudeb",
"family_name": "Saha",
"email": null,
"author_order": 10,
"ORCID": "https://orcid.org/0000-0002-9163-3801",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 250
}
]
},
{
"id": 249,
"slug": "178-1686643151-kidney-injury-molecule-1-and-cystatin-c-as-early-biomarkers-for-renal-dysfunction-in-iraqi-type-2-diabetes-mellitus-patients",
"type": "original_article",
"manuscript_id": "178-1686643151",
"published": "2023-08-31",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/03/178-1686643151.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 673-685",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Bangladesh",
"cite_info": "Yahya AA, Kadhim DJ, et al. Kidney injury molecule-1 and cystatin C as early biomarkers for renal dysfunction in Iraqi type 2 diabetes mellitus patients. J Adv Biotechnol Exp Ther. 2023; 6(3): 673-685.",
"title": "Kidney injury molecule-1 and cystatin C as early biomarkers for renal dysfunction in Iraqi type 2 diabetes mellitus patients",
"abstract": "<p>Diabetic kidney disease (DKD) is caused by a variety of processes. As a result, one biomarker is insufficient to represent the complete process. This study evaluated the diagnostic value of serum kidney injury molecule-1(KIM-1) and cystatin C (CysC) as early biochemical markers of DKD and predicted their sensitivities and specificities as biomarkers of nephropathy in Iraqi type 2 diabetic (T2DM) patients. This cross-sectional study includes 161 T2DM patients. Patients were divided according to urinary albumin creatinine ratio (ACR) such as group1: ACR≤ 30mg/g and group2: ACR> 30mg/g. Random spot urine and fasting blood samples were taken from each patient. Urinary ACR, blood glycated hemoglobin (HbA1c), and serum glucose, creatinine (SCr), lipid profile, CysC, and KIM-1 were assayed, and the estimated glomerular filtration rat (eGFR) was calculated. When compared to the normoalbuminuric group, the DKD group had significantly greater prevalence of retinopathy, and significantly elevated HbA1c and total cholesterol values. Also, DKD group had significantly greater serum levels of KIM-1 and CysC, and there was a significant positive correlation between them. In contrast, GFR was significantly higher in normoalbuminuric group and was significantly negatively correlated with both CysC and KIM-1. Multiple linear regression analysis found that there were a significant positive association among CysC and ACR. Receiver operating characteristic (ROC) analysis revealed that eGFR had the highest area under the curve (AUC=0.717), while SCr had the lowest AUC (0.556). In conclusion, serum KIM-1 and CysC levels could be considered as early biomarker for DKD along with eGFR. Additionally, there was a strong correlation between serum CysC and KIM-1 as well as other renal measures that indicate deteriorating kidney function.</p>",
"DOI": "10.5455/jabet.2023.d158",
"views": 1649,
"downloads": 267,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/02/178-1686643151-Figure1.jpg",
"caption": "Figure 1. The difference in the means of patient urine albumin and urine creatinine according to existence of DKD, in which DKD patients had significantly higher urine albumin and lower urine creatinine.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/02/178-1686643151-Figure2.jpg",
"caption": "Figure 2. The difference in the means of patient eGFR and ACR according to existence of DKD show significant higher ACR in DKD while significant higher eGFR in normalbuminuric patients. eGFR: estimated glomerular filtration rate, and ACR: albumin creatinine ratio.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/02/178-1686643151-Figure3.jpg",
"caption": "Figure 3. The difference in the means of patient KIM-1 and Cystatin C according to existence of DKD revealed significantly higher levels of both KIM-1 and CystatinC in DKD patients. KIM-1: kidney injury molecule-1.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/02/178-1686643151-Figure4.jpg",
"caption": "Figure 4. Receiver operating characteristic (ROC) curve to assess the diagnostic efficiency of four renal parameters: CysC, KIM-1, SCr, and ACR by distinguishing patient based on ACR (either normalbuminuria or DKD) in T2DM. The AUC for serum KIM-1 and CysC was comparable and better than that of SCr. CysC: cystatin C, KIM-1: kidney injury molecule-1, SCr: serum creatinine, and ACR: albumin creatinine ratio.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/02/178-1686643151-Figure5.jpg",
"caption": "Figure 5. Receiver operating characteristic (ROC) curve to assess the diagnostic efficiency of estimated glomerular filtration rate (eGFR). For eGFR, AUC was 0.717 and the cutoff value for identification of ACR≥30mg\\g was found to be 95.5 mL/min/1.73 m2 with a sensitivity of 72% and specificity of 63%.",
"featured": false
}
],
"authors": [
{
"id": 1168,
"affiliation": [
{
"affiliation": "Clinical Pharmacy, Babylon Health Directorate, Babylon, Iraq"
}
],
"first_name": "Ansam Abdulameer",
"family_name": "Yahya",
"email": "ansam.abdulameer1100e@copharm.uobaghdad.edu.iq",
"author_order": 1,
"ORCID": "https://orcid.org/0009-0001-2823-7785",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ansam Abdulameer Yahya, Clinical Pharmacy, Babylon Health Directorate, Babylon, Iraq. \r\nEmail: ansam.abdulameer1100e@copharm.uobaghdad.edu.iq",
"article": 249
},
{
"id": 1169,
"affiliation": [
{
"affiliation": "Department of Clinical Pharmacy, College of Pharmacy, University of Baghdad, Baghdad, Iraq"
}
],
"first_name": "Dheyaa Jabbar",
"family_name": "Kadhim",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-2654-1441",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 249
},
{
"id": 1170,
"affiliation": [
{
"affiliation": "Endocrinologist and Internist, Merjan Medical city, Babylon Health Directorate, Babylon, Iraq"
}
],
"first_name": "Nassar Abdalaema",
"family_name": "Abdalhadi",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0009-0004-7617-0716",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 249
}
]
},
{
"id": 248,
"slug": "178-1690735908-effects-of-glycyrrhizin-stevioside-and-sucralose-on-the-growth-and-metabolism-of-the-omnipresent-gut-commensal-escherichia-coli",
"type": "original_article",
"manuscript_id": "178-1690735908",
"published": "2023-08-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/08/178-1690735908.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 659-672",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Tuli SS, Shehreen S, et al. Effects of glycyrrhizin, stevioside, and sucralose on the growth and metabolism of the omnipresent gut commensal Escherichia coli. J Adv Biotechnol Exp Ther. 2023; 6(3): 659-672",
"title": "Effects of glycyrrhizin, stevioside, and sucralose on the growth and metabolism of the omnipresent gut commensal <span>Escherichia coli</span>",
"abstract": "<p>The rise in popularity of non-caloric sweeteners (NCS) among obese and diabetic patients is due to their ability to provide a sweet taste without any caloric input. However, NCS consumption may be linked to metabolic disorders. The mechanisms behind these adverse effects are still unclear, which suggests that NCS may alter the metabolic activity of the gut microbiome, leading to enteric environmental perturbation and resulting in physiological anomalies in the host. This study investigates how <em>Escherichia coli</em>, a common gut microbe, reacts to three NCS, two of which come from nature (glycyrrhizin and stevioside) and one that is made artificially (sucralose). To assess the impact of these NCS, this study investigated the growth, gene expression, and metabolic pathways of <em>E. coli </em>under different sweetener loads. Compared to the untreated control, low amounts of glycyrrhizin made <em>E. coli </em>grow faster, while similar amounts of sucralose and stevioside had no or a less noticeable effect. At higher concentrations, these sweeteners slowed down or inhibited the growth of the bacteria. Thus, NCS may have dose-dependent impacts on gut microbes. After treatment with amounts that corresponded to the acceptable daily intake, the expression of key metabolic genes showed altered expression, which may potentially affect their metabolism. At different concentrations of the NCS, there were clear differences in the global metabolomic profile between the control and the different NCS treatments. Under <em>in vitro</em> conditions, glycyrrhizin and sucralose appeared to have a less divergent impact on <em>E. coli</em> growth, gene expression, and metabolism, which may suggest that these are relatively safer sugar substitutes for humans.</p>",
"DOI": "10.5455/jabet.2023.d157",
"views": 2311,
"downloads": 250,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/31/178-1690735908-Figure1.jpg",
"caption": "Figure 1. Growth of E. coli in LB media supplemented with a narrower concentration range of glycyrrhizin, stevioside, and sucralose. A. E. coli was cultured under different glycyrrhizin loads (0.5 mg/ml, 1.0 mg/l, 1.8 mg/ml, 2.5 mg/ml, and 5 mg/ml). The bacterial growth curves under 0.5 mg/ml and 1 mg/ml glycyrrhizin load surpassed that of the control. At 1.8 mg/ml and higher concentrations of glycyrrhizin, bacterial growth was inhibited gradually in a dose dependent manner. B. E. coli was cultured at different concentrations of stevioside (1 mg/ml, 2.5 mg/ml, and 5 mg/ml). 1 mg/ml and 2.5 mg/ml stevioside showed growth slightly greater than the control (0 mg/ml of stevioside). C. E. coli was cultured at different was generated under different loads of sucralose (0.5 mg/mL, 1 mg/mL, 2.5 mg/mL, and 5 mg/ml). E. coli grown under 0.5 mg/ml and 1 mg/ml sucralose load showed almost similar growth as the control (0 mg/ml of sucralose). Dunnett's multiple comparisons test was applied to compare the growth of NCS-treated cells at different time points relative to the untreated control cells. Data on statistical analyses are shown in supplementary tables 1, 2, and 3.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/31/178-1690735908-Figure2.jpg",
"caption": "Figure 2. Relative gene expression in E. coli following treatment with glycyrrhizin, stevioside, and sucralose. E. coli cells were treated with 1.0 mg/ml and 1.8 mg/ml glycyrrhizin, 1.0 mg/ml and 3.5 mg/ml stevioside, and 1.0 mg/ml and 3.78 mg/ml sucralose. Expression levels were compared using Holm Sidak multiple comparison test (* denote p <0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/31/178-1690735908-Figure3.jpg",
"caption": "Figure 3. Global metabolomic profiling. A. Unsupervised multivariate analysis with metabolomic data using PCA. Metabolites from bacterial samples grown under 1.0 and 1.8 mg/ml glycyrrhizin and 3.78 mg/ml cluster closest to the metabolites from untreated bacterial samples. B. Heatmap with the top 50 metabolites based on ANOVA. Metabolites from bacterial samples grown under 1.0 mg/ml and 1.8 mg/ml glycyrrhizin, and 3.78 mg/ml show similar patterns of expression.",
"featured": false
}
],
"authors": [
{
"id": 1163,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Tuli",
"family_name": "Samayeta Sarkar",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 248
},
{
"id": 1164,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Shehreen",
"family_name": "Saadlee",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 248
},
{
"id": 1165,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Khan",
"family_name": "Abira",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 248
},
{
"id": 1166,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Akhteruzzaman",
"family_name": "Sharif",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 248
},
{
"id": 1167,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Sajib",
"family_name": "Abu Ashfaqur",
"email": "abu.sajib@du.ac.bd",
"author_order": 5,
"ORCID": "https://orcid.org/0000-0003-1710-9865",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Abu Ashfaqur Sajib, PhD; Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh. Email: abu.sajib@du.ac.bd",
"article": 248
}
]
},
{
"id": 247,
"slug": "178-1689424828-genomic-identification-and-characterization-of-prophages-associated-with-citrobacter-freundii-strains",
"type": "original_article",
"manuscript_id": "178-1689424828",
"published": "2023-08-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/35/178-1689424828.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023 Sep; 6(3): 648-658",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Jabeen I, Mahamud SMI, et al. Genomic identification and characterization of prophages associated with Citrobacter freundii strains. J Adv Biotechnol Exp Ther. 2023 Sep; 6(3): 648-658",
"title": "Genomic identification and characterization of prophages associated with <span>Citrobacter freundii</span> strains",
"abstract": "<p><em>Citrobacter freundii</em> is a Gram-negative opportunistic bacterium that can cause infections, sepsis, and meningitis, predominantly in infants and immunocompromised adults. This study is aimed at investigating the distribution of prophages in <em>C. freundii</em> strains and their potential effects on the host strains using genomic characterization. <em>In-silico</em> analysis of 144 complete chromosomal sequences of <em>C. freundii</em> strains was performed, and 574 intact prophages were identified from 1178 prophages in the host. The genome sizes of the intact prophages were 6.74-115.15 kb; on average, the host chromosomes were predicted to have around 3.98% of intact prophage genomes. At least three intact prophages were most frequently predicted, while only three host chromosomes were found to have the highest number of nine intact prophages. The GC content of prophages is 50.75%, slightly lower than the average GC content (51.85%) of <em>C. freundii. </em>Most of the prophages in <em>C. freundii</em> strains were classified into four families, Myoviridae (48.16%), Siphoviridae (42%), Podoviridae (4.67%), Inoviridae (0.17%), and 5% of the intact prophages could not be assigned into any family. Phylogenomic analysis of intact prophages divided the genomes into three distinct clades. Virulence gene analysis revealed the variable distribution of 7 virulence genes (<em>hcp</em>, <em>higB</em>, <em>hipA</em>, <em>msgA</em>, <em>rtx</em>, <em>yeeV</em>, and <em>ykfI</em>) among the intact prophages. Overall, this study provides insights into the diversity and characteristics of prophages associated with <em>C. freundii</em> strains, which will help in understanding the genetic evolution and pathogenesis of the bacteria.</p>",
"DOI": "10.5455/jabet.2023.d156",
"views": 2101,
"downloads": 348,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/25/178-1689424828-Figure1.jpg",
"caption": "Figure 1. Prevalence of intact prophages in 144 C. freundii strains. A) Average number of prophages per chromosome. Error bars indicate standard deviations. B) Distribution of 574 intact prophages according to sequence length (kb), C) Frequency of intact prophage present in C. freundii strains.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/25/178-1689424828-Figure2.jpg",
"caption": "Figure 2. Distribution of average genome size (kb) and GC % of prophages in several viral families.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/25/178-1689424828-Figure3.jpg",
"caption": "Figure 3. Spatial distribution of prophages in C. freundii genomes. All intact prophages found within C. freundii chromosomes (n = 574) were plotted on a density graph. The x-axis displayed base pair positions along the C. freundii genome, and the y-axis showed the likelihood that each base pair unit had a prophage. Thus, the area under the curve provided the prophage density for a given period along the x-axis. The resulting map revealed two peaks along the bacterial chromosome at 4900000 to 510000 bp, indicating elevated prophage density.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/25/178-1689424828-Figure4.jpg",
"caption": "Figure 4. Heatmaps displaying the toxin protein profiles in prophages associated with C. freundii genomes. The absence and presence of virulence genes are indicated by gray and white color, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/25/178-1689424828-Figure5.jpg",
"caption": "Figure 5. The phylogenomic tree of C. freundii associated 574 intact prophages using Neighbouring Joining methods with 1000 bootstrap replications.",
"featured": false
}
],
"authors": [
{
"id": 1156,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Ishrat",
"family_name": "Jabeen",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-6081-0337",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 247
},
{
"id": 1157,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "S. M. Iqbal",
"family_name": "Mahamud",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0009-0009-4969-1168",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 247
},
{
"id": 1158,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Sohidul",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-4003-2544",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 247
},
{
"id": 1159,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Anika Bushra",
"family_name": "Lamisa",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-5183-5839",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 247
},
{
"id": 1160,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Afia",
"family_name": "Anjum",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0009-0005-3260-3556",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 247
},
{
"id": 1161,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Sumaiya Hossain",
"family_name": "Oishy",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 247
},
{
"id": 1162,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Sabbir R.",
"family_name": "Shuvo",
"email": "sabbir.shuvo@northsouth.edu",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-5539-5834",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sabbir R. Shuvo, PhD; Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh. Email: sabbir.shuvo@northsouth.edu",
"article": 247
}
]
},
{
"id": 246,
"slug": "178-1685297439-no-evidence-of-relationship-between-colorectal-cancer-susceptibility-and-ercc2-gene-polymorphisms",
"type": "original_article",
"manuscript_id": "178-1685297439",
"published": "2023-07-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/55/178-1685297439.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 638-647",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Farhad R, Saleh ES, et al., No evidence of relationship between colorectal cancer susceptibility and ERCC2 gene polymorphisms. J Adv Biotechnol Exp Ther. 2023; 6(3): 638-647",
"title": "No evidence of relationship between colorectal cancer susceptibility and ERCC2 gene polymorphisms",
"abstract": "<p>Excision repair cross-complementing group 2 gene (<em>ERCC2</em>) polymorphisms have been linked as being a risk factor for colorectal cancer (CRC) emergence. However, data from several studies are contradictory. To validate genetic biomarkers of the CRC, the impact of the following <em>ERCC2</em> polymorphism (rs1799793 and rs238406) was examined on CRC susceptibility among sample of Iraqi population. A total of 126 subjects were enrolled in this case control study; 78 CRC patients and 48 apparently healthy individuals who are matched for age, gender, smoking status, and BMI. Polymerase chain reaction (PCR) was used for genotyping, followed by sequencing then the association between genetic polymorphisms and CRC risk was investigated. No associations were detected between ERCC2 genotypes or haplotypes and CRC susceptibility. Even though there was strong linkage disequilibrium (D′=0.82). After stratification according to demographics of the participants, no effects were observed for age, gender, smoking status, and body mass index (BMI). Taken together, the results suggest that <em>ERCC2</em> polymorphisms do not influence CRC development.</p>",
"DOI": "10.5455/jabet.2023.d155",
"views": 1617,
"downloads": 302,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/13/178-1685297439-Figure1.jpg",
"caption": "Figure 1. Linkage disequilibrium of ERCC2 SNPs.",
"featured": false
}
],
"authors": [
{
"id": 1153,
"affiliation": [
{
"affiliation": "Department of Clinical Pharmacy, College of Pharmacy, University of Baghdad, Baghdad, Iraq"
}
],
"first_name": "Rand",
"family_name": "Farhad",
"email": "rand.mothana1100e@copharm.uobaghdad.edu.iq",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-2517-5045",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Rand Farhad, Department of Clinical Pharmacy, College of Pharmacy, University of Baghdad, Baghdad, Iraq. Email: rand.mothana1100e@copharm.uobaghdad.edu.iq",
"article": 246
},
{
"id": 1154,
"affiliation": [
{
"affiliation": "Department of Clinical Laboratory Sciences, College of Pharmacy, University of Baghdad, Baghdad, Iraq"
}
],
"first_name": "Eman Saadi",
"family_name": "Saleh",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 246
},
{
"id": 1155,
"affiliation": [
{
"affiliation": "Oncology Teaching Hospital-Medical city, Baghdad, Iraq"
}
],
"first_name": "Ahmed Zuhair",
"family_name": "Alsammarraie",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-7419-961X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 246
}
]
},
{
"id": 245,
"slug": "178-1684487829-proniosomes-a-provesicular-system-in-ocular-drug-delivery",
"type": "review_article",
"manuscript_id": "178-1684487829",
"published": "2023-07-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/22/178-1684487829.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 622-637",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Kandpal N, Ale Y, et al. Proniosomes: A provesicular system in ocular drug delivery. J Adv Biotechnol Exp Ther. 2023; 6(3): 622-637.",
"title": "Proniosomes: A provesicular system in ocular drug delivery",
"abstract": "<p>The eyes are the only sense organ required for vision. Diseases like glaucoma, cataract, diabetic retinopathy etc. affect the proper functioning of the eyes and sometimes lead to blindness. The treatment of eye disorders is very challenging because of the unique structure of this organ. The traditional treatment approaches are not effective in providing good ocular bioavailability. The provesicular systems are new-generation delivery systems that can improve drug bioavailability and provide therapeutic responses in a controlled manner for desired time. Among all, liposomes are the first such delivery vehicle but due to the lack of stability and the high cost, niosomes were formulated. Niosomes are nanosized vesicles composed of non-ionic surfactants that can encapsulate both lipophilic and hydrophilic drugs. The drawbacks associated with niosomes, like fusion, aggregation, sedimentation, difficulty in sterilization, leaking, etc., gave birth to proniosomes. Proniosomes are more stable and bioavailable than niosomes and liposomes. Proniosomes are dry formulations of hydrophilic carrier particles coated with a water-soluble non-ionic surfactant that, when hydrated, instantly transforms into niosomes. Proniosomes can be used as stable, non-toxic carrier carriers to improve the ocular residence and bioavailability of many drugs. This paper reviewed proniosomes, their biomedical applications and their toxicity in ocular drug delivery.</p>",
"DOI": "10.5455/jabet.2023.d154",
"views": 2341,
"downloads": 351,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/13/178-1684487829-Figure_1.jpg",
"caption": "Figure 1. Conventional versus novel approaches for ocular drug delivery.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/13/178-1684487829-Figure_2.jpg",
"caption": "Figure 2. Factors affecting the formulation of proniosomes.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/13/178-1684487829-Figure_3.jpg",
"caption": "Figure 3. Proniosomes represent a promising drug delivery option for ocular drug delivery. The schema summarizes the potential application of proniosomes to overcome the problems associated with conventional dosage forms and their application in the treatment of various ocular conditions like conjunctivitis, glaucoma, keratitis, uveitis, etc.",
"featured": false
}
],
"authors": [
{
"id": 1146,
"affiliation": [
{
"affiliation": "Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, 248007, India"
}
],
"first_name": "Neha",
"family_name": "Kandpal",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 245
},
{
"id": 1147,
"affiliation": [
{
"affiliation": "Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, 248007, India"
}
],
"first_name": "Yogita",
"family_name": "Ale",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 245
},
{
"id": 1148,
"affiliation": [
{
"affiliation": "GRD(PG)IMT, Rajpur Road, Dehradun 248009, Uttarakhand, India"
}
],
"first_name": "Yamini Chandola",
"family_name": "Semwal",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 245
},
{
"id": 1149,
"affiliation": [
{
"affiliation": "Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, 248007, India"
}
],
"first_name": "Neha",
"family_name": "Padiyar",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 245
},
{
"id": 1150,
"affiliation": [
{
"affiliation": "Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, 248007, India"
}
],
"first_name": "Vikash",
"family_name": "Jakhmola",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 245
},
{
"id": 1151,
"affiliation": [
{
"affiliation": "Himalayan Institute of Pharmaceutical Sciences, Swami Ram Himalayan University, Jolly Grant, Dehradun, 248016, India"
}
],
"first_name": "Arvind Singh",
"family_name": "Farswan",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 245
},
{
"id": 1152,
"affiliation": [
{
"affiliation": "Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Premnagar, 248007, India"
}
],
"first_name": "Nidhi",
"family_name": "Nainwal",
"email": "nidhi.nainwal87@gmail.com",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0003-0117-1701",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nidhi Nainwal, PhD; Uttaranchal Institute of Pharmaceutical Sciences, \r\nUttaranchal University, Premnagar, 248007, India. Email: nidhi.nainwal87@gmail.com",
"article": 245
}
]
},
{
"id": 244,
"slug": "178-1681206450-cloning-and-expression-of-recombinant-purine-nucleoside-phosphorylase-in-the-methylotrophic-yeast-pichia-pastoris",
"type": "original_article",
"manuscript_id": "178-1681206450",
"published": "2023-07-18",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/57/178-1681206450.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 610-621",
"academic_editor": "Mohammad Nazrul Islam, PhD; Sher-e-Bangla Agricultural University, Bangladesh",
"cite_info": "Abdurakhmanov J, Sasmakov S, et al. Cloning and expression of recombinant purine nucleoside phosphorylase in the methylotrophic yeast Pichia pastoris . J Adv Biotechnol Exp Ther. 2023; 6(3): 610-621.",
"title": "Cloning and expression of recombinant purine nucleoside phosphorylase in the methylotrophic yeast <span>Pichia pastoris</span>",
"abstract": "<p>Purine nucleoside phosphorylase (PNP) is an enzyme involved in biosynthetic pathway of purine nucleosides. Purine nucleoside phosphorylase catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides to form the purine base and deoxyribose- or ribose-1-phosphate. The reversible reaction catalyzed by recombinant PNP of <em>E. coli</em> (<em>Ec</em>PNP) has been successfully used for the synthesis of nucleoside analogues. The aim of this study was to clone and expression of recombinant PNP in <em>Pichia pastoris.</em> For expression of recombinant <em>Ec</em>PNP in <em>Pichia pastoris,</em> a new recombinant plasmid DNA pPICZαA-PNP (4256 bp) containing the deoD gene (720 bp) amplified from <em>E. coli</em> strain RKMUz-221 has been cloned. The substrate specificity of the recombinant <em>Ec</em>PNP expressed in yeast cells was studied by hydrolysis of inosine (9-beta-D-ribofuranosylhypoxanthine) to hypoxanthine and 2-deoxy-β-D-ribofuranosyl. The fraction containing the obtained recombinant EcPNP (≈28 kDa, as determined by SDS-PAGE) demonstrates significant hydrolytic activity, resulting in up to a 51% hydrolysis of inosine within a 5 h timeframe. These findings suggest that the recombinant enzyme holds a promise for applications in enzymatic transglycosylation of purine-type nucleosides.</p>",
"DOI": "10.5455/jabet.2023.d153",
"views": 1556,
"downloads": 190,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/14/178-1681206450-Figure1.jpg",
"caption": "Figure 1. Physical map of the recombinant plasmid pPICZαA-PNP.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/14/178-1681206450-Figure2.jpg",
"caption": "Figure 2. PCR analysis of the recombinant E. coli Top10 clones: (A) Clones; (B) К- (pPICZαA); (C) DNA marker.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/14/178-1681206450-Figure3.jpg",
"caption": "Figure 3. Restriction analysis of the recombinant plasmid pPICZαA-PNP and original plasmid by identical restriction site: (A) pPICZαA-TP/SacI (4256 bp); (B) pPICZαA/SacI (3593 bp); (C) DNA Marker.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/14/178-1681206450-Figure4.jpg",
"caption": "Figure 4. The alignment of the cloned EcPNP sequence with the sequences of representative E. coli (BM28) from GenBank.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/14/178-1681206450-Figure5.jpg",
"caption": "Figure 5. SDS-PAGE of fraction containing rEcPNP: (A) Tri-color restrained protein marker I (10-180kD), Bioland Scientific; (B) fraction after gradient precipitation with 25%-65% ammonium sulphate (3 and 5 μg protein per well).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/14/178-1681206450-Figure6.jpg",
"caption": "Figure 6. Enzymatic hydrolysis of Inosine (9-beta-D-ribofuranosylhypoxanthine) by purine nucleoside phosphorylase (PNP).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/14/178-1681206450-Figure7.jpg",
"caption": "Figure 7. Time-course of enzymatic hydrolysis of Inosine catalyzed by recombinant EcPNP expressed in P. pastoris. The graph shows the percentage of inosine hydrolyzed over a time period of 0 to 5 h.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/14/178-1681206450-Figure8.jpg",
"caption": "Figure 8. HPLC analysis of enzymatic hydrolysis of Inosine catalyzed by recombinant PNP. i-ii) The quantity of hydrolyzed Inosine in reaction mixture over time from 0 min to 5h.",
"featured": false
}
],
"authors": [
{
"id": 1133,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Jaloliddin",
"family_name": "Abdurakhmanov",
"email": "jaloliddin0919@mail.ru",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-3954-3251",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Jaloliddin Abdurakhmanov, PhD; S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan, Email: jaloliddin0919@mail.ru",
"article": 244
},
{
"id": 1134,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Sobirdjan",
"family_name": "Sasmakov",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-1863-7117",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1135,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Shukhrat",
"family_name": "Khasanov",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-4773-7117",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1136,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Oybek",
"family_name": "Ashirov",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-0855-6105",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1137,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Farkhod",
"family_name": "Eshboev",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0003-3054-6111",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1138,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Khayotjon",
"family_name": "Dolimov",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1139,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Saodat",
"family_name": "Gaynazarova",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1140,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Tulkin",
"family_name": "Sadullaev",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1141,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Oygul",
"family_name": "Yarilkaganova",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1142,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Khusan",
"family_name": "Nasriddinov",
"email": null,
"author_order": 10,
"ORCID": "https://orcid.org/0000-0002-9074-0533",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1143,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Asadali",
"family_name": "Baymirzaev",
"email": null,
"author_order": 11,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1144,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Artyom",
"family_name": "Makhnyov",
"email": null,
"author_order": 12,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
},
{
"id": 1145,
"affiliation": [
{
"affiliation": "S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Mirzo Ulugbek Str. 77, 100170, Tashkent, Uzbekistan"
}
],
"first_name": "Shakhnoz",
"family_name": "Azimov",
"email": null,
"author_order": 13,
"ORCID": "https://orcid.org/0000-0003-1803-7728",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 244
}
]
},
{
"id": 243,
"slug": "178-1683613081-molecular-characterization-of-multidrug-resistant-bacteria-isolated-from-the-external-and-internal-parts-of-the-housefly",
"type": "original_article",
"manuscript_id": "178-1683613081",
"published": "2023-07-16",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/05/178-1683613081.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 597-609",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University, Japan",
"cite_info": "Zuhora FT, Hosen MA, et al., Molecular characterization of multidrug-resistant bacteria isolated from the external and internal parts of the housefly. J Adv Biotechnol Exp Ther. 2023; 6(3): 597-609.",
"title": "Molecular characterization of multidrug-resistant bacteria isolated from the external and internal parts of the housefly",
"abstract": "<p>House flies are mechanical vectors of food borne enteric pathogenic bacteria which may transfer isolates to human and produce diseases. In Bangladesh, there is very limited data on molecular characterization of drug-resistant bacteria from isolated house flies. The research was conducted to determine the pathogenic bacteria isolated from houseflies and their antibiogram. A total of 140 houseflies were randomly collected for microbiological analysis. A group of cultural tests, biochemical tests were used to isolation and identification of isolates and further confirmed through molecular characterization by the presence of 16S r<em>RNA</em> gene E1, E2 and <em>invA</em>. Additionally, 14 commercially available antibiotics were used by karby-bauer disk diffusion technique for antibiogram study. Results showed that the most isolated bacteria from houseflies' external surfaces were <em>Escherichia coli</em> 19.04%, <em>Salmonella typhimurium </em>15.87%, and <em>Pseudomonas </em>spp. 7.93% from 63 isolates, while 42 isolates found 35.71%, 28.57%, and 14.28% respectively. PCR amplification bands of <em>Escherichia coli</em>, <em>Salmonella typhimurium</em>, and <em>Pseudomonas</em> spp. were 584bp, 284bp, and 1497bp, respectively. Almost all of the isolates were highly resistant to erythromycin, gentamycin, bacitracin (100%), followed by kanamycin, methicillin (80%) whereas highly sensitive to ciprofloxacin, chloramphenicol, azithromycin (100%), followed by tetracycline, amoxicillin (85.71%). These pathogenic microorganisms at distinct sampling sites indicate that house flies may transmit vector-borne pathogens to humans. Based on these findings, we recommend vector-borne disease-fighting medications and a sustainable house fly-control approach. We also suggest promoting hygiene and food safety protocol to distance food ingredient from flies in local markets.</p>",
"DOI": "10.5455/jabet.2023.d152",
"views": 1421,
"downloads": 389,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/14/178-1683613081-Figure1.jpg",
"caption": "Figure 1. Cultural characteristics of Staphylococcus spp. on MSA (A), Klebsiella spp. on MacConkey agar (B), Salmonella spp. on SS agar (C) and E. coli on EMB agar (D), Biochemical test result of E. coli positive in TSI (E), Salmonella spp. positive in TSI (F), Salmonella spp. positive in Citrate test (G), and E. coli negative in Citrate test (H).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/14/178-1683613081-Figure2.jpg",
"caption": "Figure 2. Amplification of E. coli by E1 and E2 Primer, Ladder 1: test sample, Lane M: Marker- 1000 bp E. coli showed 584 bp band.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/14/178-1683613081-Figure3.jpg",
"caption": "Figure 3. The phylogenetic tree made from 16S ribosomal RNA of E. coli from housefly which differ from others.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/14/178-1683613081-Figure4.jpg",
"caption": "Figure 4. Amplification of Salmonella typhimurium by s139 and s141 primer. Test sample of Salmonella typhimurium showed band at 284 bp and Lane M: Marker (50 bp).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/14/178-1683613081-Figure5.jpg",
"caption": "Figure 5. The phylogenic tree made from 16S ribosomal RNA of Salmonella typhimurium from housefly which also differ from others.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/14/178-1683613081-Figure6.jpg",
"caption": "Figure 6. Amplification of Pseudomonas spp. by ker P (F) and ker P (R). Test sample of Pseudomonas spp. showed band at 1497bp and Lane M: Marker (1kb).",
"featured": false
}
],
"authors": [
{
"id": 1127,
"affiliation": [
{
"affiliation": "Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Fatema Tuz",
"family_name": "Zuhora",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-6954-1380",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 243
},
{
"id": 1128,
"affiliation": [
{
"affiliation": "Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Aoulad",
"family_name": "Hosen",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-0147-5169",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 243
},
{
"id": 1129,
"affiliation": [
{
"affiliation": "Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Nazmi Ara",
"family_name": "Rumi",
"email": "rumi_dvm@yahoo.com",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0001-7989-8850",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nazmi Ara Rumi, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur- 5200, Bangladesh. Email: rumi_dvm@yahoo.com",
"article": 243
},
{
"id": 1130,
"affiliation": [
{
"affiliation": "Department of Medicine, Surgery and Obstetrics, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Shajedur",
"family_name": "Rahman",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0003-4820-8639",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 243
},
{
"id": 1131,
"affiliation": [
{
"affiliation": "Animal Biotechnology Division, National Institute of Biotechnology, Ashulia, Dhaka-1349, Bangladesh"
}
],
"first_name": "Jahangir",
"family_name": "Alam",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0001-5439-1412",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 243
},
{
"id": 1132,
"affiliation": [
{
"affiliation": "Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Hasibul",
"family_name": "Hasan",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-1259-3355",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 243
}
]
},
{
"id": 242,
"slug": "178-1684085734-association-of-gut-microbiota-in-the-pathogenesis-of-acute-respiratory-distress-syndrome-and-possible-pharmacotherapy-by-sphingosine-1-phosphate",
"type": "review_article",
"manuscript_id": "178-1684085734",
"published": "2023-07-15",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1684085734.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 584-596",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Amin T, Zahed FB. Association of gut microbiota in the pathogenesis of acute respiratory distress syndrome and possible pharmacotherapy by sphingosine-1-phosphate. J Adv Biotechnol Exp Ther. 2023; 6(3): 584-596.",
"title": "Association of gut microbiota in the pathogenesis of acute respiratory distress syndrome and possible pharmacotherapy by sphingosine-1-phosphate",
"abstract": "<p>The acute respiratory distress syndrome (ARDS) is one of the major heterogeneous clinical manifestations of severe respiratory failure developing in response to pathogenetic mechanisms and several inflammatory insults. The translocation of gut microbiota has a crucial impact on the pathogenesis of ARDS. Hence, a deeper understanding of the interplay of the gut microbiota would allow shedding valuable insights into both the pathogenesis of ARDS and the development of effective therapeutic interventions. Moreover, the modulation of gut-lung axis could also play a pivotal role in minimizing the lung dysbiosis with gut microbiota. There is little question that greater study of the gut-lung axis in critically ill patients is required to establish causal links between the shifted microbiota, infections, inflammation, and acute lung damage. It is worth mentioning that the lack of effective preventative measures is one of the main reasons for the increased mortality rate of 30-40% in ARDS patients. Some antibiotics and nanosized drugs showed positive results in ARDS management to some extent in pre-clinical or even first stages of clinical trials but large-scale results had been controversial. However, sphingosine-1-phosphate (S1P) showed hopeful results in ARDS patients by facilitating both systemic and endothelial integrity. Numerous investigations have shown the immunological connection between the gastrointestinal tract and the respiratory system. As the SphKs /S1P /S1PL metabolic pathway is associated with a wide variety of human illnesses (including respiratory diseases), it should come as no surprise that influencing intracellular S1P levels would have therapeutic promise in reducing the severity of lung diseases like ARDS.</p>",
"DOI": "10.5455/jabet.2023.d151",
"views": 1781,
"downloads": 441,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/32/14/178-1684085734-Figure1.jpg",
"caption": "Figure 1. Role of pulmonary microbiota in both health and critical illness.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/32/14/178-1684085734-Figure2.jpg",
"caption": "Figure 2. S1P signaling. In the cytoplasm and nucleus, both SphK1 and SphK2 are activated by different ligands which leads to the production of S1P. The dephosphorylation of S1P is carried out by phosphatases generates sphingosine or it can be cleaved irreversibly into ethanolamine and hexadecenal phosphate by S1P lyase as well. S1P interacts to one of the five G-protein-coupled receptors (S1PR1-5) on cell membranes before being taken out of the cell by ABC transporters or Spns2. It activates a wide range of downstream signaling pathways.",
"featured": false
}
],
"authors": [
{
"id": 1125,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Tasbir",
"family_name": "Amin",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-5265-8769",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 242
},
{
"id": 1126,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Fahd Bin",
"family_name": "Zahed",
"email": "fahd.zahed@northsouth.edu",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-2687-190X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Fahd Bin Zahed; Department of Biochemistry and Microbiology, North South University, Bashundhara, Dhaka-1229, Bangladesh. Email: fahd.zahed@northsouth.edu",
"article": 242
}
]
},
{
"id": 241,
"slug": "178-1685574680-association-of-serum-level-of-interleukin-33-and-insulin-resistance-in-overt-and-subclinical-hypothyroidism-patients",
"type": "short_communication",
"manuscript_id": "178-1685574680",
"published": "2023-07-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/12/178-1685574680.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 575-583",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Jabbar ZAH, Ali HA, et al. Association of serum level of interleukin-33 and insulin resistance in overt and subclinical hypothyroidism patients. J Adv Biotechnol Exp Ther. 2023; 6(3): 575-583.",
"title": "Association of serum level of interleukin-33 and insulin resistance in overt and subclinical hypothyroidism patients",
"abstract": "<p>Hypothyroidism is a condition that occurs when the thyroid gland fails to produce an adequate amount of the hormone thyroid, as a result of various factors such as hypothalamic or pituitary gland disease, common tissue resistance to thyroid hormones, and thyroid gland diseases. Hypothyroidism is the most prevalent thyroid disorder. Interleukin (IL)-33 is a nuclear cytokine of the IL-1 family members that is abundantly expressed during homeostasis and inflammation in endothelial, epithelial, and fibroblast-like cells. It is worth noting that higher IL-33 levels have been associated with insulin resistance. The purpose of the study was to examine the correlation between insulin resistance and IL-33 in patients with hypothyroidism. In this case-control study, 180 people were recruited and split into three categories: those with overt hypothyroidism (60), those with subclinical hypothyroidism (60), and those who were otherwise healthy control (60). There were of a similar age range to the patient, with 55.5% female participants and 44.5% male participants. Standard ELISA kits assess insulin, IL-33, thyroid stimulating hormone (TSH), thyroxine (T4), and triiodothyronine (T3). The healthy control group showed lower serum IL-33 levels than individuals with both overt and subclinical hypothyroidism. Compared to the healthy control group, all hypothyroidism groups had higher BMI, serum TSH, fasting glycemic glucose (FSG), insulin, triglyceride (TG), cholesterol levels (Chol), very low-density lipoprotein (VLDL), bad cholesterol (LDL), IL-33, and homeostatic model assessment of insulin resistance (HOMA-IR). In hypothyroid patients, IL-33 levels were negatively correlated with HOMA-IR and HDL-C and positively correlated with body mass index, TSH, FSG, insulin, HOMA-IR, total cholesterol, LDL, and total cholesterol. Increased levels of circulating IL-33 in the serum of patients with hypothyroidism are linked to alterations in lipid profiles and HOMA-IR. Hypothyroid patients, especially those with obvious symptoms showed elevated levels of IL-33, suggesting a possible role for this cytokine in detecting the metabolic shifts that precede these complications.</p>",
"DOI": "10.5455/jabet.2023.d150",
"views": 1770,
"downloads": 332,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/00/14/178-1685574680-Figure1.jpg",
"caption": "Figure 1. Comparison between levels of IL-33 and overt or subclinical hypothyroidism patients, as well as in healthy males and females.",
"featured": false
}
],
"authors": [
{
"id": 1121,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science, University of Kufa, Najaf 54001, Iraq"
}
],
"first_name": "Zainab Abdul Hussein",
"family_name": "Jabbar",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 241
},
{
"id": 1122,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science, University of Kufa, Najaf 54001, Iraq"
}
],
"first_name": "Hanaa Addai",
"family_name": "Ali",
"email": "muthanahana74@gmail.com",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Hanaa Addai Ali, Department of Chemistry, Faculty of Science, University of Kufa, Najaf 54001, Iraq, e-mail: muthanahana74@gmail.com",
"article": 241
},
{
"id": 1123,
"affiliation": [
{
"affiliation": "Microbiology Department, College of Veterinary Medicine, Al-Qasim Green University, Babylon 51001, Iraq"
}
],
"first_name": "Rawaa Adday",
"family_name": "Ali",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 241
},
{
"id": 1124,
"affiliation": [
{
"affiliation": "Department of Chemistry, Faculty of Science, University of Kufa, Najaf 54001, Iraq"
}
],
"first_name": "Mohauman Mohammed Majeed",
"family_name": "AlRufaie",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 241
}
]
},
{
"id": 240,
"slug": "178-1676921940-assessing-newborn-screening-practices-in-bangladesh-perspectives-of-healthcare-professionals-and-implications-for-improved-infant-health",
"type": "original_article",
"manuscript_id": "178-1676921940",
"published": "2023-06-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/09/178-1676921940.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 564-574",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Sumaia SN, Reshad RAI, et al. Assessing newborn screening practices in Bangladesh: Perspectives of healthcare professionals and implications for improved infant health. J Adv Biotechnol Exp Ther. 2023; 6(3): 564-574.",
"title": "Assessing newborn screening practices in Bangladesh: Perspectives of healthcare professionals and implications for improved infant health",
"abstract": "<p>Newborn screening (NBS) plays a crucial role in identifying and managing congenital disorders, ultimately leading to improved infant health outcomes. This study aims to assess the current practices of newborn screening in Bangladesh, focusing on the perspectives of healthcare professionals, and identifying potential implications for enhancing infant health. A quantitative research design was employed, utilizing in-depth interviews and focus group discussions with healthcare professionals involved in newborn care across various healthcare settings. Findings indicate that newborn screening practices in Bangladesh face several challenges, including limited awareness among healthcare professionals, inadequate infrastructure and resources, and the absence of a comprehensive national screening program. Furthermore, financial constraints, cultural beliefs, and social barriers contribute to the low utilization of screening services by families. The perspectives of healthcare professionals shed light on potential strategies to address these challenges and improve newborn screening practices in Bangladesh. These strategies include enhancing healthcare professionals' knowledge and skills through training programs, increasing public awareness about the importance of newborn screening, strengthening healthcare infrastructure and accessibility, and implementing a comprehensive national screening program. The implications of improving newborn screening practices are significant. Early detection of congenital disorders through NBS enables timely interventions, leading to reduced morbidity, mortality, and long-term disabilities in infants. Enhanced screening practices can also contribute to reducing the burden on healthcare systems by preventing complications associated with undiagnosed conditions and enabling more cost-effective management. This study highlights the need for comprehensive efforts to address the challenges faced in newborn screening practices in Bangladesh. By incorporating the perspectives of healthcare professionals, policymakers can develop targeted interventions to enhance newborn screening programs, thereby improving infant health outcomes and ensuring a healthier future for Bangladesh's newborn population.</p>",
"DOI": "10.5455/jabet.2023.d149",
"views": 1429,
"downloads": 305,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/14/178-1676921940-Figure_1.jpg",
"caption": "Figure 1. Distribution of participants by gender and workplace type. This figure illustrates the distribution of participants in the study based on their gender and workplace type.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/14/178-1676921940-Figure_2.jpg",
"caption": "Figure 2. Knowledge level of health professionals on newborn screening (NBS). This figure presents the distribution of knowledge levels among health professionals surveyed regarding NBS.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/14/178-1676921940-Figure_3.jpg",
"caption": "Figure 3. Attitudes towards cost-effectiveness of newborn screening by present occupation. The figure represents the distribution of attitudes towards the cost-effectiveness of Newborn screening among participants based on their present occupation. The data indicates that medical students exhibited a higher belief in the cost-effectiveness of Newborn screening compared to participants with other occupations. The significance of the relationship between occupation and the perception of cost-effectiveness was determined to be statistically significant (p<0.05).",
"featured": false
}
],
"authors": [
{
"id": 1113,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology. School of Life Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Sayedatun Nesa",
"family_name": "Sumaia",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 240
},
{
"id": 1114,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology. School of Life Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Riyan Al Islam",
"family_name": "Reshad",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 240
},
{
"id": 1115,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology. School of Life Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Fawzia",
"family_name": "Tabassum",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 240
},
{
"id": 1116,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Daffodil International University, Dhaka, Bangladesh"
}
],
"first_name": "Sabrina Khan",
"family_name": "Mim",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 240
},
{
"id": 1117,
"affiliation": [
{
"affiliation": "Department of Internal Medicine, Jalalabad Ragib Rabeya Medical College and Hospital, Sylhet, Bangladesh"
}
],
"first_name": "Mohammad Golam Rob",
"family_name": "Mahmud",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 240
},
{
"id": 1118,
"affiliation": [
{
"affiliation": "Department of Cardiology, MAG Osmani Medical College and Hospital, Sylhet, Bangladesh"
}
],
"first_name": "Chowdhury Muhammad Omar",
"family_name": "Faruque",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 240
},
{
"id": 1119,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology. School of Life Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Gokul Chandra",
"family_name": "Biswas",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 240
},
{
"id": 1120,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology. School of Life Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Md. Faruque",
"family_name": "Miah",
"email": "faruque-btc@sust.edu",
"author_order": 8,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Faruque Miah, PhD; Department of Genetic Engineering and Biotechnology Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh, e-mail: faruque-btc@sust.edu",
"article": 240
}
]
},
{
"id": 239,
"slug": "178-1682573716-effect-of-white-tea-camellia-sinensis-leaf-extract-on-cigarette-smoke-and-high-fat-diet-induced-atherosclerosis-in-wistar-rats",
"type": "original_article",
"manuscript_id": "178-1682573716",
"published": "2023-06-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/40/178-1682573716.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 552-563.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka 1230, Bangladesh",
"cite_info": "Faustin J, Bahrudin M, et al. Effect of white tea (Camellia sinensis) leaf extract on cigarette smoke and high-fat diet-induced atherosclerosis in Wistar rats. J Adv Biotechnol Exp Ther. 2023; 6(3): 552-563.",
"title": "Effect of white tea (Camellia sinensis) leaf extract on cigarette smoke and high-fat diet-induced atherosclerosis in Wistar rats",
"abstract": "<p>Atherosclerosis is a complex pathological process begins with endothelial dysfunction, one of which is triggered by an inflammatory process due to increased levels of low-density lipoprotein (LDL) cholesterol and exposure to cigarette smoke. White tea leaf (<em>Camellia sinensis</em>) shows an anti-inflammatory effect which has not been known for its effect on the atherosclerotic process. The aim of this study is to determine the effect of <em>Camellia sinensis</em> leaf extract on IL-6 levels, foam cell count, and the ratio of intima-media thickness of cigarette smoke and high-fat diet-induced atherosclerosis in Wistar rats. Thirty male Wistar rats were randomly divided into 5 groups namely HC (healthy control), NC (negative control), WT100, WT200, and WT400 (treated with white tea leaf extract 100, 200, and 400 mg/kgBW/day, respectively). IL-6 levels were measured by the ELISA method. At 400x microscope magnification, foam cell count and intima-media thickness ratio were seen in aortic tissue. Administration of graded doses of white tea leaf extract in groups WT100, WT200, WT400 significantly reduced IL-6 levels, foam cell count, and intima-media thickness ratio of abdominal aorta compared to the NC group. These results suggest that white tea leaf extract may decrease levels of IL-6, foam cell count, and intima-media thickness ratio in atherosclerosis-induced Wistar rats.</p>",
"DOI": "10.5455/jabet.2023.d148",
"views": 1602,
"downloads": 257,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/14/178-1682573716-Figure_1.jpg",
"caption": "Figure 1. Effect of white tea leaf extract on body weight in rats. According to the results of a one-way ANOVA test, there was no significant difference in body weight between groups. HC (healthy control), NC (negative control), WT100, WT200, and WT400 (white tea leaf extract 100/200/400 mg/kgBW/day), n=6.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/14/178-1682573716-Figure_2.jpg",
"caption": "Figure 2. Effect of white tea leaf extract on blood IL-6 levels in rats. The chart shows the results of the post-hoc test, which indicated significant differences in IL-6 levels between groups. HC (healthy control), NC (negative control), WT100, WT200, and WT400 (white tea leaf extract 100/200/400 mg/kgBW/day), n=6. # = significant (p < 0,05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/14/178-1682573716-Figure_3.jpg",
"caption": "Figure 3. The effect of white tea leaf extract on foam cell count in rats. The chart shows the results of the post-hoc test, which indicated significant differences in foam cell count between groups. HC (healthy control), NC (negative control), WT100, WT200, and WT400 (white tea leaf extract 100/200/400 mg/kgBW/day), n=6. # = significant (p < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/14/178-1682573716-Figure_4.jpg",
"caption": "Figure 4. Effect of white tea leaf extract on abdominal aorta (400x magnification). Abdominal aorta cross slice displaying tunica intima foam cells (black arrow). HC (healthy control), NC (negative control), WT100, WT200, and WT400 (white tea leaf extract 100, 200, 400 mg/kgBW/day), n=6.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/14/178-1682573716-Figure_5.jpg",
"caption": "Figure 5. Effect of white tea leaf extract on the intima-media thickness ratio in rats. The chart shows the results of the post-hoc test, which indicated significant differences in intima-media thickness ratio between groups. HC (healthy control), NC (negative control), WT100, WT200, and WT400 (white tea leaf extract 100/200/400 mg/kgBW/day), n=6. # = significant (p < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/14/178-1682573716-Figure_6.jpg",
"caption": "Figure 6. Effect of white tea leaf extract on the thickness of tunica intima in rats. The chart shows the results of the post-hoc test, which indicated significant differences in the thickness of tunica intima between groups. HC (healthy control), NC (negative control), WT100, WT200, and WT400 (white tea leaf extract 100/200/400 mg/kgBW/day), and n=6. # = significant (p < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/14/178-1682573716-Figure_7.jpg",
"caption": "Figure 7. Charts show the correlation between variables (A) IL-6 levels and foam cell count, (B) IL-6 levels and intima-media thickness ratio, (C) foam cells count and intima-media thickness ratio. *=significant.",
"featured": false
}
],
"authors": [
{
"id": 1107,
"affiliation": [
{
"affiliation": "Graduate School of Biomedical Science, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Jennifer",
"family_name": "Faustin",
"email": "dean@fk.undip.ac.id",
"author_order": 1,
"ORCID": "http://orcid.org/0009-0007-9806-4473",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Jennifer Faustin, PhD; Graduate School of Biomedical Science, Faculty of Medicine Diponegoro University, Semarang, Central Java, Indonesia, \r\ne-mail: dean@fk.undip.ac.id",
"article": 239
},
{
"id": 1108,
"affiliation": [
{
"affiliation": "Department of Histology, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Md.",
"family_name": "Bahrudin",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 239
},
{
"id": 1109,
"affiliation": [
{
"affiliation": "Department of Cardiology, Faculty of Medicine, Diponegoro University, Central Java, Indonesia"
}
],
"first_name": "Neni",
"family_name": "Susilaningsih",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 239
},
{
"id": 1110,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutics, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Muflihatul",
"family_name": "Muniroh",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 239
},
{
"id": 1111,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutics, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Endang",
"family_name": "Mahati",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 239
},
{
"id": 1112,
"affiliation": [
{
"affiliation": "Department of Pathological Anatomy, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Hermawan",
"family_name": "Istiadi",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 239
}
]
},
{
"id": 238,
"slug": "178-1677590380-restoration-of-hepatorenal-dysfunction-and-injury-by-zinc-and-folic-acid-combination-in-bisphenol-a-intoxicated-mice",
"type": "original_article",
"manuscript_id": "178-1677590380",
"published": "2023-06-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/31/178-1677590380.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(3): 541-551.",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Mustari A, Alam M, et al. Restoration of hepatorenal dysfunction and injury by zinc and folic acid combination in bisphenol A-intoxicated mice . J Adv Biotechnol Exp Ther. 2023; 6(3): 541-551.",
"title": "Restoration of hepatorenal dysfunction and injury by zinc and folic acid combination in bisphenol A-intoxicated mice",
"abstract": "<p>Bisphenol A (BPA) is a potentially hazardous substance and is extensively used in manufacturing industries as a plasticizing agent. The current research intended to determine the revival actions of zinc (Zn) and folic acid (FA) on hematological parameters and hepatorenal function in BPA-exposed male albino mice. A total of 75 adult male mice, aged 25 to 28 days, were split into 5 groups. Group A (control group) received a normal diet, while a daily dosage of 50 mg BPA/kg body weight (BW) in the diet was provided to groups B–E. Groups C, D, and E received daily supplementation of 10 mg Zn/kg BW, 3 mg FA/kg BW, or both in the feed. After 12 weeks of treatment, serum was prepared by collecting blood, and the kidney and liver were taken for a histotexture study. The results demonstrated that hematological values dropped significantly (<em>p</em>< 0.05) in the BPA-treated group and increased following Zn and FA supplementations, while no significant alterations in erythrocyte indices were observed among control and treated groups. The BPA group used to have significantly (<em>p</em>< 0.05) higher liver and kidney biomarkers levels, which were restored by Zn and FA. The effects of BPA administration included severe hepatocyte degeneration and necrosis, as well as renal tubule epithelium loss, all of which were improved by Zn and FA. Hence, the findings suggest that combination of Zn and FA could be potential preventative agents against BPA-induced toxicity.</p>",
"DOI": "10.5455/jabet.2023.d147",
"views": 2296,
"downloads": 299,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/09/14/178-1677590380-Figure_1.jpg",
"caption": "Figure 1. Effects of Zn and FA supplementation on hematological parameters in BPA-exposed male mice. Mice were kept untreated (Group A) or treated with BPA (Group B), Zn (Group C), FA (Group D) or both Zn and FA (Group E) and the values of (a) TEC, (b) Hb, (c) PCV, (d) MCV, (e) MCH and (f) MCHC were measured. Data indicate mean ± SEM of 3 replicates. Each replicate contains pooled blood of five mice (n=5). One-way ANOVA with Bonferroni multiple comparison tests was performed. Here, ***=Statistically significant at 0.1% level (p≤ 0.001), **=Statistically significant at 1% level (p≤ 0.01), *= Statistically significant at 5% level (p ≤0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/09/14/178-1677590380-Figure_2.jpg",
"caption": "Figure 2. Effects of Zn and FA supplementation on liver function biomarkers of BPA-exposed mice. Mice were kept untreated (Group A) or treated with BPA (Group B), Zn (Group C), FA (Group D) or both Zn and FA (Group E) and the values of (a) AST (b) ALT and (c) ALP were measured. Data indicate mean ± SEM of 3 replicates. Each replicate contains pooled sera of five mice (n=5). One-way ANOVA with Bonferroni multiple comparison tests was performed. Here, ***=Statistically significant at 0.1% level (p≤ 0.001), **=Statistically significant at 1% level (p≤ 0.01), *= Statistically significant at 5% level (p≤ 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/09/14/178-1677590380-Figure_3.jpg",
"caption": "Figure 3. Effects of Zn and FA supplementation on histoarchitecture of liver of BPA-exposed mice. (a) Section of liver of control mice (Group A) showing normal architecture. (b) Section of liver of BPA-exposed mice (Group B) showing severe degenerative and necrotic changes (DNC) of hepatocytes. Sections of the liver of mice fed with (c) Zn (Group C), (d) FA (Group D) and (e) both Zn and FA (Group E) show preservation of normal architecture of the hepatocytes in the liver. Haematoxylin and eosin stain, bar = 50 µm indicate magnification.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/09/14/178-1677590380-Figure_4.jpg",
"caption": "Figure 4. Effects of Zn and FA supplementation on kidney function biomarkers of BPA-exposed mice. Mice were kept untreated (Group A) or treated with BPA (Group B), Zn (Group C), FA (Group D) or both Zn and FA (Group E) and the values of (a) serum creatinine and (b) Total protein (TP) were measured. Data indicate mean ± SEM of 3 replicates. Each replicate contains pooled sera of five mice (n=5). One-way ANOVA with Bonferroni multiple-comparison tests was performed. Here, ***=Statistically significant at 0.1% level (p≤ 0.001), **=Statistically significant at 1% level (p≤ 0.01), *= Statistically significant at 5% level (p≤ 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/09/14/178-1677590380-Figure_5.jpg",
"caption": "Figure 5. Effects of Zn and FA supplementation on histoarchitecture of kidney of BPA-exposed mice. (a) Section of kidney of control mice (Group A) showing normal architecture. (b) Section of kidney of BPA-exposed mice (Group B) showing severe loss of lining epithelium (LLE) of the renal tubule. Sections of the kidney of mice fed with (c) Zn (Group C), (d) FA (Group D), and (e) both Zn and FA (Group E), show the preservation of the normal architecture of the kidney. Haematoxylin and eosin stain, bar = 50 µm indicate magnification.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/09/14/178-1677590380-Figure_6.jpg",
"caption": "Figure 6. Schematic diagram is showing the effects of Zn and FA supplementation on hematological parameters and hepatorenal functions in BPA-exposed mice. Mice exposed to BPA were kept untreated (control) or treated with Zn, FA or both Zn and FA.",
"featured": false
}
],
"authors": [
{
"id": 1101,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Afrina",
"family_name": "Mustari",
"email": "afrina.mustari@bau.edu.bd",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-7651-5487",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Afrina Mustari, Department of Physiology, Faculty of Veterinary science, \r\nBangladesh Agricultural University, Mymensigh-2202, Bangladesh.\r\nEmail: afrina.mustari@bau.edu.bd",
"article": 238
},
{
"id": 1102,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Mahabub",
"family_name": "Alam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 238
},
{
"id": 1103,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Mohammad Alam",
"family_name": "Miah",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0003-2778-9614",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 238
},
{
"id": 1104,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Khaled Mahmud",
"family_name": "Sujan",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0003-3330-1662",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 238
},
{
"id": 1105,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "A.G.M.Sofi Uddin",
"family_name": "Mahamud",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-5779-6359",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 238
},
{
"id": 1106,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh"
}
],
"first_name": "Emdadul Haque",
"family_name": "Chowdhury",
"email": null,
"author_order": 6,
"ORCID": "http://orcid.org/0000-0002-8212-7923",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 238
}
]
}
]
},
{
"id": 19,
"title": "Vol6 Issue2",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2023",
"slug": "2023-vol6-issue2",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 227,
"slug": "178-1679777735-cytotoxic-effect-of-zinc-oxide-nanoparticle-and-diode-laser-combination-in-colorectal-cancer-in-vitro-via-induction-of-cell-cycle-arrest-and-apoptosis",
"type": "original_article",
"manuscript_id": "178-1679777735",
"published": "2023-05-29",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/47/178-1679777735.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 527-540.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Rahman CS, Mustafa TA. Cytotoxic effect of zinc oxide nanoparticle and diode laser combination in colorectal cancer in vitro via induction of cell cycle arrest and apoptosis. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 527-540.",
"title": "Cytotoxic effect of zinc oxide nanoparticle and diode laser combination in colorectal cancer in vitro via induction of cell cycle arrest and apoptosis",
"abstract": "<p>The fourth deadliest reason for cancer-related deaths worldwide was found to be colorectal cancer. Zinc oxide nanoparticles (ZnO NPs) have been researched for biomedical applications due to their demonstrated anticancer, antidiabetic, antibacterial, and anti-inflammatory activities. The cytotoxic reactive oxygen species (ROS) that are produced when photosensitizers (PSs) are activated by light, which in turn cause cancer cell death, and photodynamic treatment (PDT) causes selective cytotoxicity to malignant cells. The cytotoxic effect of different concentrations of nanoparticles and laser wavelengths was studied on colorectal cancer cell line (CaCo2), while the use of combination therapy had clear effects on early apoptosis. The half-maximal inhibitory concentration (IC50) of nanoparticles for the cells was evaluated. The proper IC50 for CaCo2 cells was 20 ug/ml Zinc oxide nanoparticle and Diode laser power is 5 j/cm2. The percentage of cells that decreased in the G2 phase of the cell cycle relative to the S and G1 phases was significantly different when the combination of laser and zinc nanoparticles was used. Combined use of a diode laser and ZnO<sub>2</sub> significantly inhibited the spread of cancer by boosting the expression of the p53 gene. Bax, a central cell death regulator, was expressed significantly in combined treatments. However, combined use of the laser and ZnO<sub>2</sub> did not reveal any substantial differences in the Bcl2 gene. In conclusion, this study suggests that combination therapy was cytotoxic to CaCo2 cells in vitro and there is a possibility of developing it as an effective therapeutic agent.</p>",
"DOI": "10.5455/jabet.2023.d146",
"views": 1819,
"downloads": 293,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/16/178-1679777735-Figure1.jpg",
"caption": "Figure 1. Effect of Zinc nanoparticles, laser, and nano-laser combinations on apoptosis in in CaCo2 cells. The dose of nanoparticle was 20 ug/ml, and laser was 5 j/cm2 (0.5W and 10 s). Cytotoxicity was measured using MTT assay. All groups compare with control group.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/16/178-1679777735-Figure2.jpg",
"caption": "Figure 2. Effect of Zinc nanoparticles, laser, and nano-laser combinations on apoptosis and necrosis in in CaCo2 cells. Cells were treated for 24h, 48h, and 72h). *Q1: Necrosis, Q2: Late Apoptosis, Q3: Early Apoptosis, Q4: Live Cells. The dose of nanoparticle was 20 ug/ml, and laser was 5 j/cm2 (0.5W and 10 s). These were measured using flow cytometry.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/16/178-1679777735-Figure3.jpg",
"caption": "Figure 3. The graph illustrates the influence of nano, laser, and nano-laser combinations on the proportion of CaCo2 cells in different cell cycle phases. The dose of nanoparticle was 20 ug/ml, and laser was 5 j/cm2 (0.5W and 10 s). These were measured using flow cytometry. All groups compare with control group.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/16/178-1679777735-Figure4.jpg",
"caption": "Figure 4. Effect of Zinc nanoparticles, laser, and nano-laser combinations on remaining the amount of DNA contents in CaCo2 cells with different cell cycle phases. The dose of nanoparticle was 20 ug/ml, and laser was 5 j/cm2 (0.5W and 10 s). These were measured using flow cytometry.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/16/178-1679777735-Figure5.jpg",
"caption": "Figure 5. Effect of Zinc nanoparticles, laser, and nano-laser combinations on reactive oxygen species (ROS) using DCFH+ and DCFH- in CaCo2 cells. The dose of nanoparticle was 20 ug/ml, and laser was 5 j/cm2 (0.5W and 10 s). ROS were measured using flow cytometry. All groups compare with control group.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/16/178-1679777735-Figure6.jpg",
"caption": "Figure 6. Effect of zinc nanoparticles, laser in alone and zinc nanoparticles with laser on the percent of CaCo2 cells in both DCFH+ and DCFH-. The dose of nanoparticle was 20 ug/ml, and laser was 5 j/cm2 (0.5W and 10 s). ROS were measured using flow cytometry. All groups compare with control group.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/16/178-1679777735-Figure7.jpg",
"caption": "Figure 7. Graph revealed the effects of nano, laser, and nano-laser combination in the expression of apoptotic genes in in CaCo2 cells. The dose of nanoparticle was 20 ug/ml, and laser was 5 j/cm2 (0.5W and 10 s). Gene expression was measured using Real-time PCR method. All groups compare with control group.",
"featured": false
}
],
"authors": [
{
"id": 1046,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory, Erbil Health and Medical Technical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Chrakhan Salh",
"family_name": "Rahman",
"email": "chraxan.gardy@gmail.com",
"author_order": 1,
"ORCID": "http://orcid.org/0009-0008-6684-6080",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Chrakhan Salh Rahman, Department of Medical Laboratory, Erbil Health and Medical Technical College, Erbil Polytechnic University, Erbil, Iraq, e-mail: chraxan.gardy@gmail.com",
"article": 227
},
{
"id": 1047,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory, Erbil Health and Medical Technical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Twana Ahmed",
"family_name": "Mustafa",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0001-6067-7695",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 227
}
]
},
{
"id": 226,
"slug": "178-1678179076-serological-and-oncoinformatic-analysis-of-hba1c-as-a-prognostic-biomarker-in-screening-the-risks-of-different-cancers-among-the-male-t2d-patients-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1678179076",
"published": "2023-05-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/14/178-1678179076.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 510-526.",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University Bangladesh",
"cite_info": "Hasibuzzaman MA, Alam HMI, et al. Serological and oncoinformatic analysis of HbA1c as a prognostic biomarker in screening the risks of different cancers among the male T2D patients of Bangladesh.J Adv Biotechnol Exp Ther. 2023 May; 6(2): 510-526.",
"title": "Serological and oncoinformatic analysis of HbA1c as a prognostic biomarker in screening the risks of different cancers among the male T2D patients of Bangladesh",
"abstract": "<p>Hemoglobin A1C (HBA1c) represents the average serological sugar status of the T2D patients of the past three months, considered a clinically standard method of studying sugar metabolism. Overexpressing HbA1 can metabolically forecast the risk of different cancers among the T2D patients. Based on which, the study aimed to analyze the impact of sugar metabolism in cancer development considering the overexpression of HbA1 as the prognostic biomarker of screening the risks of eight different cancers among the chronic male T2D patients of Bangladesh. Serological analysis of the concentrations of FBS, THABF, creatinine, SC, STGs, HDLC, and LDLC of the T2D patients were conducted in response to their individual HbA1c concentration. Afterward, HbA1 overexpression and promotor-methylation responsible for BLCA, BRCA, CHOL, COAD, LUAD, LUSC, PAAD, and PRAD cancers in the male T2D patients were profiled as the oncoinformatic screening, where the sample types used, individual cancer stages, racial-footprints, gender, age, nodal metastasis, p53-methylations, pancreatitis, diabetes status, smoking behaviors, and survivability status were studied. Finally, the genetic involvement of a group of genes responsible for genetic co-expression of HbA1, endophytic vesicle regulation, antioxidant regulation, and reactive oxygen species based-metabolic regulation in T2D males were identified and comprehensively discussed. The research revealed, significant correlation between BMI and FBS in both the patient and the control groups (p<0.0001). Besides, FBS, THABF, and creatinine were found significantly regulated with their respective HbA1c concentrations (p<0.0001) for each group. The SC, STGs, HDLC, and LDLC regulated ardently and equally for both groups (p<0.0001), while HbA1c ranged from 3.8-5.8% and 5.11-15.8%, for the controls and patients respectively. HbA1 was found interactive with diversified cancer-causing genes, while HbA1 was mostly downregulating with the progressing metastasis. To receive maximum benefits from using HbA1c in clinically profiling of cancer-risks among the chronic-male T2D patients in minimal time and expense further studies can be needed with larger sample size.</p>",
"DOI": "10.5455/jabet.2023.d145",
"views": 1558,
"downloads": 217,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/16/178-1678179076-Figure1.jpg",
"caption": "Figure 1. The correlation between BMI (Kg/m2) and FBG (mmol/L) among the patients (A) and control group (B). In all the cases, a strong correlation was found for both groups (p < 0.0001) on the scale of significance of α= 0.05. The linear regression line validates the ranges of BMI and FBS for each group precisely. In these figures, p indicates patients and C indicates the control group. The value of standard error for T2-DM Patients was calculated at 0.07467 and for the control group at 0.08381. The 95% Confident Interval for T2-DM patients were between 3.160-3.454 and that of the control group was between 3.389-3.724.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/16/178-1678179076-Figure2.jpg",
"caption": "Figure 2. The correlations of HbA1c (%) with the patients’ FBS (mmol/L) (A); THABF (mmol/L) (B) and creatinine (mg/dL) (C) in comparison to their corresponding control groups on the same parameters (D-F). In all the individual parameters of the patients and controls, significant correlations were observed (p < 0.0001). THABF indicated Two Hours After Breakfast. For the patient group, the Standard Error (SE) of HbA1c Vs FBS was calculated at 0.02380, and the 95% Confidence Interval (CI) value was between 0.7601 and 0.8538. SE of HbA1c vs THABF was 0.03560 whereas the 95% CI was between 1.109 and 0.1339. Lastly, the SE of HbA1c vs Creatinine was 0.004939 and the value of 95% CI was between 0.1145 and 0.1339. On the other hand, in the case of the control group, the SE value for HbA1c VS FBS was 0.03408 and the 95% CI value was between 1.021 and 1.158. SE value for HbA1c VS THABF in the control group was 0.03333 and the 95% CI value was between 1.056 and 1.189. Finally, the SE for HbA1c Vs Creatinine in the control group was 0.007136 and 95% CI was between 0.1788 and 0.2074. In all these cases, the scale of significance was α = 0.05, and the p-value was < .0001 on this scale.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/16/178-1678179076-Figure3.jpg",
"caption": "Figure 3. Comparative analysis of different serological parameters in response to the individuals’ HbA1c concentration. The correlations of individual SC (mg/dl), STGs (mg/dl), HDLC (mg/dl), and LDLC (mg/dl) in response to the HbA1c (%) for the T2D patients (A-C) and the control individuals (D-F) have been illustrated, where the R square values were determined in addition to the SE and 95% CI values for all. The scale of significance was considered α = 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/16/178-1678179076-Figure4.jpg",
"caption": "Figure 4. Profiling of the HbA1 gene expression among cancer patients in comparison to the normal individuals. Five different cancer types were considered, where among the breast cancers- bladder urothelial carcinoma (BLCA), breast invasive carcinoma (BRCA); colon cancers- cholangiocarcinoma tumor (CHOL), colon adenocarcinoma (COAD); lung cancers- lung adenocarcinoma (LUAD), lung squamous cell carcinoma (LUSC); pancreatic cancer- pancreatic adenocarcinoma (PAAD); and prostate cancer- prostate adenocarcinoma (PRAD) were considered.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/16/178-1678179076-Figure5.jpg",
"caption": "Figure 5. Multifactorial influence in profiling the expression of HbA1 among the individuals. In that case, factors like sample types (A), individual cancer stages of patients (B), regional expression (C), gender specificity (D), age factor (E), nodal metastasis (F), level of the p53 mutation (G), pancreatitis status of the diabetes patients (H), and the diabetes status (T2D) (I) were considered.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/16/178-1678179076-Figure6.jpg",
"caption": "Figure 6. The profiling of the HbA1 –promotor methylation (HbA1P*) in risk-profiling of different cancers among the T2D patients studied. To analyze comprehensively some parameters such as cell types (A), cancer stages (B), regional expression (C), gender specificity (D), age factor (E), tumor grades (F), p53 expression (G), nodal metastasis (H), and smoking habits (I) were preferred. The Cancer Genome Atlas (TCGA) referred that the HbA1 gene expression level (beta-value) remains static within the range of 0.081 to 0.121 for normal individuals whereas the level fluctuates dynamically as compared to normal persons depending on the aforementioned parameters.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/16/178-1678179076-Figure7.jpg",
"caption": "Figure 7. Analysis of the HBA1-mediated gene-gene interaction (GGI) inside the complicated cellular systems in human. Parameters like overall GGI regulated by the HbA1 gene (A); HbA1 genetic co-expression with diabetes and cancer gene (B); gene involved in vesicle formation (C); Antioxidant regulation (D); oxygen species metabolic regulation (E); and gene-mediated response to the reactive oxygen molecules (F) were considered for a comprehensive study. Different protein-coding genes are represented including Alpha-hemoglobin-stabilizing protein (AHSP); Apolipoprotein A1 (APOA1), Aquaporin-1 (AQP1), Carbonic anhydrase 1 (CA1), Carbonic anhydrase 2 (CA2), Calumenin (CALU), Scavenger receptor cysteine-rich type 1 protein M130 (CD163), Cytochrome b5 reductase 3 (CYB5R3), Hemoglobin subunit beta (HBB), Hemoglobin subunit delta (HBD), Hemoglobin subunit mu (HBM), Hemoglobin subunit zeta (HBZ), Hemoglobin subunit alpha (HBA1), Hemoglobin subunit alpha 2 (HBA2), Hemoglobin Subunit Gamma 1 (HBG1), Hemoglobin subunit gamma-2 (HBG2), haptoglobin (HP), Haptoglobin-related protein (HPR), Hemopexin (HPX), and Rh-associated glycoprotein (RHAG) coding genes.",
"featured": false
}
],
"authors": [
{
"id": 1029,
"affiliation": [
{
"affiliation": "School of Medicine, Ningbo University, Ningbo City-315211, PR China"
},
{
"affiliation": "Public Health and Medicine Research Unit, RPG Interface Lab, Jashore-7400, Bangladesh"
}
],
"first_name": "Md. Al",
"family_name": "Hasibuzzaman",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0003-0548-4836",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1030,
"affiliation": [
{
"affiliation": "Division of Validation, BEXIMCO Pharma Ltd., Dhaka-1205, Bangladesh"
}
],
"first_name": "H.M. Iftekhar",
"family_name": "Alam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1031,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan-25200, Malaysia"
},
{
"affiliation": "Public Health and Medicine Research Unit, RPG Interface Lab, Jashore-7400, Bangladesh"
}
],
"first_name": "Md. Abdur Rashid",
"family_name": "Mia",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-0954-4501",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1032,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
},
{
"affiliation": "Public Health and Medicine Research Unit, RPG Interface Lab, Jashore-7400, Bangladesh"
}
],
"first_name": "Samarth",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0002-7959-4598",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1033,
"affiliation": [
{
"affiliation": "Department of Biological Science, Illinoise state university, Illinois-61761, USA"
}
],
"first_name": "Sabiha",
"family_name": "Sultana",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0001-9577-0371",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1034,
"affiliation": [
{
"affiliation": "Department of Pharmacology, Jashore Medical College, Jashore, 7400, Bangladesh"
},
{
"affiliation": "Public Health and Medicine Research Unit, RPG Interface Lab, Jashore-7400, Bangladesh"
}
],
"first_name": "Sharmin",
"family_name": "Ahmed",
"email": "dr.sharmin@rpgilab.org",
"author_order": 6,
"ORCID": "http://orcid.org/0000-0003-1890-457X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sharmin Ahmed, Department of Pharmacology, Jashore Medical College, Jashore-7400, Bangladesh, e-mail: dr.sharmin@rpgilab.org",
"article": 226
},
{
"id": 1035,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Afsana",
"family_name": "Masud",
"email": null,
"author_order": 7,
"ORCID": "http://orcid.org/0000-0003-4763-4687",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1036,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Samiur",
"family_name": "Rahman",
"email": null,
"author_order": 8,
"ORCID": "http://orcid.org/0000-0001-6482-5085",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1037,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Arysha Alif",
"family_name": "Khan",
"email": null,
"author_order": 9,
"ORCID": "http://orcid.org/0000-0002-6003-657X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1038,
"affiliation": [
{
"affiliation": "Department of Medicine and Surgery, Chittagong Medical College, Chittagong-4203, Bangladesh"
}
],
"first_name": "Fahmida Hoque",
"family_name": "Rimti",
"email": null,
"author_order": 10,
"ORCID": "http://orcid.org/0000-0002-7441-6733",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1039,
"affiliation": [
{
"affiliation": "Department of Medicine and Surgery, Sir Salimullah Medical College and Mitford Hospital, University of Dhaka, Dhaka-1206, Bangladesh"
}
],
"first_name": "Ashrak Shad",
"family_name": "Pyash",
"email": null,
"author_order": 11,
"ORCID": "http://orcid.org/0000-0003-2025-8589",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1040,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore-7408, Bangladesh"
},
{
"affiliation": "Immunoinformatics and Vaccinomics Research Unit, RPG Interface Lab, Jashore-7400, Bangladesh"
}
],
"first_name": "Partha",
"family_name": "Biswas",
"email": null,
"author_order": 12,
"ORCID": "http://orcid.org/0000-0002-9766-756X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1041,
"affiliation": [
{
"affiliation": "Department of Medicine and Surgery, Sir Salimullah Medical College and Mitford Hospital, University of Dhaka, Dhaka-1206, Bangladesh"
}
],
"first_name": "Homayra Rahman",
"family_name": "Shoshi",
"email": null,
"author_order": 13,
"ORCID": "http://orcid.org/0000-0003-2232-9678",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1042,
"affiliation": [
{
"affiliation": "State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi-214122, Jiangsu, PR China"
}
],
"first_name": "Mahbuba",
"family_name": "Siddiquy",
"email": null,
"author_order": 14,
"ORCID": "http://orcid.org/0000-0001-9239-4710",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1043,
"affiliation": [
{
"affiliation": "Department of Medicine and Surgery, Chittagong Medical College, Chittagong-4203, Bangladesh"
}
],
"first_name": "Fariha Hoque",
"family_name": "Rimu",
"email": null,
"author_order": 15,
"ORCID": "http://orcid.org/0000-0001-6033-4406",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1044,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka-1229, Bangladesh"
}
],
"first_name": "Ramiza Nausheen",
"family_name": "Zaman",
"email": null,
"author_order": 16,
"ORCID": "http://orcid.org/0000-0003-2025-8589",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
},
{
"id": 1045,
"affiliation": [
{
"affiliation": "Data Science Research Unit, RPG Interface Lab, Jashore-7400, Bangladesh"
}
],
"first_name": "Mst. Umme",
"family_name": "Habiba",
"email": null,
"author_order": 17,
"ORCID": "http://orcid.org/0000-0002-1639-6703",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 226
}
]
},
{
"id": 225,
"slug": "178-1678043423-effect-of-caffeine-loaded-silver-nanoparticles-on-minerals-concentration-and-antibacterial-activity-in-rats",
"type": "original_article",
"manuscript_id": "178-1678043423",
"published": "2023-05-21",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/57/178-1678043423.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 495-509.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Rasheed M, Saleem MMNM, et al. Effect of caffeine-loaded silver nanoparticles on minerals concentration and antibacterial activity in rats. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 495-509.",
"title": "Effect of caffeine-loaded silver nanoparticles on minerals concentration and antibacterial activity in rats",
"abstract": "<p>Caffeine boosts metabolism and the neurological system. When extreme weakness or sleepiness occurs, it is used recreationally and medicinally to reduce physical and mental fatigue. Caffeine initially stimulates the central nervous system, increasing intellect, speed, accuracy, focus, and coordination. The aim of the study was to evaluate how caffeine nanoparticles affect potassium, calcium, zinc, and magnesium levels, in addition, the anti-bacterial activity of the samples has been measured. Eighteen male albino rats were divided into 3 separate groups. The first group (G1) was made up of 6 animals that served as a control group. The second group (G2) was made up of 6 animals that were given caffeine, and the third group (G3) was made up of 6 animals that were given silver nanoparticles from a caffeine solution. The particle size and structural morphology of caffeine and silver nanoparticles were analyzed using Brookhaven Instruments Corp., XRD and SEM, respectively. The structural results showed that after addition, caffeine was tube-shaped, and silver was spherical granular. Caffeine has more silver nanoparticles than caffeine solution. Caffeine solution affects potassium, calcium, zinc, and magnesium levels. Additionally, the solution has antibacterial activity against the following bacteria: <em>Staphylococcus aureus</em> (Gram +ve), <em>E. coli</em> (Gram –ve), and <em>Pseudomonas aeruginosa</em> (Gram-ve), but it has no effect against yeast (<em>Candida albicans</em>). It is concluded that caffeine-synthesized Ag NPs has significant biological effects on zinc, magnesium, calcium, and potassium levels in male albino mice and this also is antibacterial against <em>Staphylococcus</em>. <strong> </strong></p>",
"DOI": "10.5455/jabet.2023.d144",
"views": 1544,
"downloads": 258,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/16/178-1678043423-Figure1.jpg",
"caption": "Figure 1. 2D to 3D molecular structure of caffeine (A, B) and adenosine (C, D), respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/16/178-1678043423-Figure2.jpg",
"caption": "Figure 2. Distribution of sample particle sizes: silver NPs (black color), caffeine NPs (red color) and (silver+caffeine) NPs (blue color) using standard laser beam=35 mW red diode laser, nominal 640 nm wavelength.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/16/178-1678043423-Figure3.jpg",
"caption": "Figure 3. X-ray spectrum for caffeine NPs.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/16/178-1678043423-Figure4.jpg",
"caption": "Figure 4. XRD analysis of silver NPs, α=β=90o, γ=120o.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/16/178-1678043423-Figure5.jpg",
"caption": "Figure 5. SEM images of caffeine: (A) 5, (B) 10, and (C) 50 µm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/16/178-1678043423-Figure6.jpg",
"caption": "Figure 6. SEM images for silver NPs: (A) 5, (B) 10, (C) 100 µm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/16/178-1678043423-Figure7.jpg",
"caption": "Figure 7. Antibacterial activity of (Caffeine with Ag) NPs show the diameter of the zone inhibition created by (caffeine +Ag NPs) with a higher dose 10-1 10-5 mg at 37 oC and after 24 h with against different microbial isolates. (A) S. aureus, (B) E. coli, (C) Pseudomonas aeruginosa, (D) Candida albicans.",
"featured": false
}
],
"authors": [
{
"id": 1020,
"affiliation": [
{
"affiliation": "Department of Applied Sciences, University of Technology- Iraq, Baghdad, Iraq"
},
{
"affiliation": "MOLTECH Anjou, Universite d’Angers/UMR CNRS 6200, 2, Bd Lavoisier, 49045 Angers, France"
}
],
"first_name": "Mohammed",
"family_name": "Rasheed",
"email": "rasheed.mohammed40@yahoo.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-0768-2142",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammed Rasheed, Department of Applied Sciences, University of Technology- Iraq, Baghdad, Iraq, e-mail: ccc",
"article": 225
},
{
"id": 1021,
"affiliation": [
{
"affiliation": "Department of Applied Sciences, University of Technology- Iraq, Baghdad, Iraq"
}
],
"first_name": "Maysoon MNM",
"family_name": "Saleem",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 225
},
{
"id": 1022,
"affiliation": [
{
"affiliation": "Department of Applied Sciences, University of Technology- Iraq, Baghdad, Iraq"
}
],
"first_name": "Thorria R.",
"family_name": "Marzoog",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 225
},
{
"id": 1023,
"affiliation": [
{
"affiliation": "Nanotechnology and Advanced Research Center, University of Technology-Iraq, Baghdad, Iraq"
}
],
"first_name": "Malaa M.",
"family_name": "Taki",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 225
},
{
"id": 1024,
"affiliation": [
{
"affiliation": "Laboratory of Active Components and Materials, Larbi Ben M'Hidi University, Oum El Bouaghi 04000, Algeria"
}
],
"first_name": "Dikra",
"family_name": "Bouras",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 225
},
{
"id": 1025,
"affiliation": [
{
"affiliation": "Nanotechnology and Advanced Research Center, University of Technology-Iraq, Baghdad, Iraq"
}
],
"first_name": "Israa A.",
"family_name": "Hashim",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 225
},
{
"id": 1026,
"affiliation": [
{
"affiliation": "Department of Biology, Science College, University of Al-Anbar, Iraq"
}
],
"first_name": "Mustafa Nuhad Al",
"family_name": "Darraji",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 225
},
{
"id": 1027,
"affiliation": [
{
"affiliation": "Al-Shirqat General Hospital, Salah Al-Deen Directorate of Health Government, Ministry of Health, Iraq"
}
],
"first_name": "Raad",
"family_name": "Rasheed",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 225
},
{
"id": 1028,
"affiliation": [
{
"affiliation": "Department of Mathematics Science, College of Science, Mustansiriyah University, Baghdad, Iraq"
}
],
"first_name": "Mohammed Abdulhadi",
"family_name": "Sarhan",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 225
}
]
},
{
"id": 224,
"slug": "178-1677941099-dmrt1-rbmy-and-azfb-genes-polymorphism-and-expression-role-in-azoospermia-susceptibility",
"type": "original_article",
"manuscript_id": "178-1677941099",
"published": "2023-05-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/28/178-1677941099.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 483-494.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Saleh AH, Dastmalchi N, et al. DMRT1, RBMY, and AZFb genes polymorphism and expression role in azoospermia susceptibility. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 483-494.",
"title": "DMRT1, RBMY, and AZFb genes polymorphism and expression role in azoospermia susceptibility",
"abstract": "<p>Male infertility can occur due to spermatogenesis defects. The most common reasons for male infertility are azoospermia and oligospermia, which have several underlying factors, one of which is genetic. This study was aimed to investigate the association of azoospermia with the <em>DMRT1</em> and <em>RBMY1A1</em> genes polymorphisms and <em>AZFb</em> region microdeletions in Iranian men. Expression of these genes was assessed by RT-PCR. A total of 100 Iranian men with azoospermia, oligozoospermia, or severe oligozoospermia and 100 fertile controls were included in this case-control study. Subjects were genotyped for <em>DMRT1</em> rs755383 and <em>RBMY1A1</em> rs1481942953 polymorphisms using Tetra-ARMS PCR. The existence of two sequence-tagged sites (STSs) on the <em>AZFb</em> region was also investigated by multiplex PCR. RT-PCR was used to analyze the expression in the testis of azoospermia cases. With a p-value of 0.038, rs755383 in the <em>DMRT1</em> gene was related to an elevated risk of azoospermia. However, no significant difference was found in genotype distribution in the <em>RBMY1A1</em> (rs1481942953) gene polymorphism. Four cases demonstrated Y chromosome microdeletions with sY127 and sY134 markers. Infertile males’ cDNA analysis revealed low expression levels for DMRT1 and <em>PRY</em> (one of the main genes in the <em>AZFb</em> region) with a p-value<0.0001. In contrast, RBMY1A1 expression level did not differ between patients and control groups with a p-value of 0.112. ROC curve analysis was done to detect genes with biomarker potential. With AUCs of 83% and 77%, DMRT1 and PRY had diagnostic marker potential in azoospermia detection.</p>",
"DOI": "10.5455/jabet.2023.d143",
"views": 942,
"downloads": 162,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/15/178-1677941099-Figure1.jpg",
"caption": "Figure 1. Genotype frequency of DMRT1 and PBMY1A1 in 100 infertile males.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/15/178-1677941099-Figure2.jpg",
"caption": "Figure 2. Decreased gene expression levels of A) the DMRT1 gene (P-value<0.0001, 95% confidence interval (95% CI) 0.777 to 0.889) and B) the PRY gene (P-value<0.0001, 95% CI 0.706 to 0.835) are statistically considerable. However, C) RBMY1A1 gene expression level did not show significant alteration in infertile males and healthy controls (P-value=0.112, 95% CI 0.484 to 0.646).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/15/178-1677941099-Figure3.jpg",
"caption": "Figure 3. By analyzing A) DMRT1 and B) PRY ROC curves, with respectively AUC= 83%, sensitivity=81%, specificity =73%, and AUC=77%, sensitivity =71%, and specificity =75%; these genes may have a potential biomarker role in infertility diagnosis. In contrast, C) RBMY1A1 did not compose any diagnostic potential (AUC=56%, sensitivity =61%, and specificity =60%).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/15/178-1677941099-Figure4.jpg",
"caption": "Figure 4. Subjects exhibited significant (p<0.001) variations in ratios of LH, FSH, and testosterone serum levels compared with the proven fathers’ group.",
"featured": false
}
],
"authors": [
{
"id": 1016,
"affiliation": [
{
"affiliation": "Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran"
}
],
"first_name": "Azeez Hasan",
"family_name": "Saleh",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 224
},
{
"id": 1017,
"affiliation": [
{
"affiliation": "Department of Biology, University College of Nabi Akram, Tabriz, Iran"
}
],
"first_name": "Narges",
"family_name": "Dastmalchi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 224
},
{
"id": 1018,
"affiliation": [
{
"affiliation": "Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran"
}
],
"first_name": "Parisa",
"family_name": "Banamolaei",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 224
},
{
"id": 1019,
"affiliation": [
{
"affiliation": "Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran"
}
],
"first_name": "Reza",
"family_name": "Safaralizadeh",
"email": "safaralizadeh@tabrizu.ac.ir",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Reza Safaralizadeh, Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, 29th Bahman Blvd., Tabriz, Iran, e-mail: safaralizadeh@tabrizu.ac.ir",
"article": 224
}
]
},
{
"id": 223,
"slug": "178-1677952781-antimicrobial-resistance-understanding-the-mechanism-and-strategies-for-prevention-and-control",
"type": "review_article",
"manuscript_id": "178-1677952781",
"published": "2023-05-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/13/178-1677952781.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 468-482.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University Bangladesh",
"cite_info": "Ali MH, Reshad RAI, et al. Antimicrobial resistance: Understanding the mechanism and strategies for prevention and control. J Adv Biotechnol Exp Ther. 2023; 6(2): 468-482.",
"title": "Antimicrobial resistance: Understanding the mechanism and strategies for prevention and control",
"abstract": "<p>Antimicrobial resistance (AMR) is a growing public health concern globally, with the threat of a post-antibiotic era, where common infections can become fatal, a very plausible reality. Despite ongoing efforts to control AMR, both mortality and expenses have increased. To combat this threat, a thorough understanding of the mechanisms and the driver behind this issue needs to be known. The key mechanisms of resistance are modification or destruction of antimicrobials, reduction of access to the target, and alteration of the target. These mechanisms may be present in the microorganisms naturally or may have been acquired from other microorganisms. As AMR jeopardizes the successful prevention and treatment of many infectious diseases, this article looks at the causes of AMR, along with the possible mechanisms of resistance development, and suggests control strategies to deal with the problem conclusively.</p>",
"DOI": "10.5455/jabet.2023.d142",
"views": 4960,
"downloads": 576,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/15/178-1677952781-Figure1.jpg",
"caption": "Figure 1. Flowchart showing the plausible transmission routes of ARGs and ARBs through the environment and reaching humans involving the agricultural context. This flowchart is a simplified representation of the complex transmission pathways of ARGs and ARBs in the agricultural context. The actual transmission routes may vary depending on several factors, including the type of antibiotic used, the mode of administration, the type of animal or crop, and the environmental conditions.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/15/178-1677952781-Figure2.jpg",
"caption": "Figure 2. Antimicrobial resistance (AMR) can arise through different pathways. This figure provides an overview of the common pathways involved in the origin of AMR. These include: (1) Enzymatic modification or degradation of antimicrobial agent, (2) Decreased uptake of antimicrobial agent, (3) Changed antimicrobial target, (4) Efflux of antimicrobial agents, and (5) A combination of all above. Addressing this problem requires a coordinated effort across different sectors, including human and veterinary medicine, agriculture, and environmental health.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/15/178-1677952781-Figure3.jpg",
"caption": "Figure 3. Schematic representation of the Main mechanisms of antimicrobial resistance. Antimicrobial resistance can occur through various mechanisms, including enzymatic inactivation of the antimicrobial agent, Modification of the antimicrobial target, Reduced permeability of the bacterial cell membrane, Efflux pumps that pump the antimicrobial agent out of the bacterial cell. This figure summarizes the major mechanisms of antimicrobial resistance and can be used as a reference for understanding how resistance can occur and potentially be addressed.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/15/178-1677952781-Figure4.jpg",
"caption": "Figure 4. Policy framework of antimicrobial resistance.",
"featured": false
}
],
"authors": [
{
"id": 1012,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet,Bangladesh"
}
],
"first_name": "Md. Hazrat",
"family_name": "Ali",
"email": "hazratali-geb@sust.edu",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Hazrat Ali, Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet,\r\nBangladesh, e mail : hazratali-geb@sust.edu",
"article": 223
},
{
"id": 1013,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet,\r\nBangladesh"
}
],
"first_name": "Riyan Al Islam",
"family_name": "Reshad",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 223
},
{
"id": 1014,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet,Bangladesh"
}
],
"first_name": "Md Muhibur",
"family_name": "Rahman",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 223
},
{
"id": 1015,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet,Bangladesh"
}
],
"first_name": "Md. Faruque",
"family_name": "Miah",
"email": "faruque-btc@sust.edu",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Faruque Miah, Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh, e-mail: faruque-btc@sust.edu",
"article": 223
}
]
},
{
"id": 221,
"slug": "178-1679245421-inorganic-arsenic-exposure-during-pregnancy-affects-post-natal-growth-blood-parameters-and-organ-development-of-mice-offspring",
"type": "original_article",
"manuscript_id": "178-1679245421",
"published": "2023-05-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/50/178-1679245421.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 445-457.",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Basher MK, Sarkar S, et al. Inorganic arsenic exposure during pregnancy affects post-natal growth, blood parameters, and organ development of mice offspring. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 445-457.",
"title": "Inorganic arsenic exposure during pregnancy affects post-natal growth, blood parameters, and organ development of mice offspring",
"abstract": "<p>Arsenic is a potentially toxic agent for human health due to its widespread presence in the environment. Arsenic poisoning from drinking contaminated groundwater has become one of Bangladesh’s most difficult healthcare problems. However, there is a lack of understanding of the detrimental impact of arsenic toxicity on children of arsenic-exposed parents. This study evaluates the effect of arsenic toxicity on body growth, blood parameters, and organ development of F1 mice. In this study, adult female mice were exposed to sodium arsenite from gestation day 12.5 until parturition, and then the postnatal growth, blood indices, and organ development were assessed. The result showed that from birth to weaning offspring of the sodium arsenite-treated group exhibited significant and weaning to sexual maturity of female offspring significantly slower increase in final body weight, total body weight gain, and rate of body weight gain than that of the offspring of the control group. Hematological tests revealed a significant reduction in RBC and WBC count while significant elevation in platelet count, MCV, MCH, and MCHC in offspring of sodium arsenite exposed female mice than the offspring obtained from control female mice. RBS and triglycerides levels were significantly higher, while alkaline phosphatase level was significantly lower in the offspring of sodium arsenite-exposed female mice than in the offspring obtained from control female mice. In terms of organ-to-body weight ratio, the female F1 mice from the exposed group demonstrated a significantly lighter kidney and heart as compared to that of the female F1 mice from the control group. Visual inspection of the organ morphology showed a slightly affected liver, lungs, and testes. Overall, the study suggests that inorganic arsenic exposure of the parent mice exerts harmful effects on the body growth, hematological, and biochemical parameters, and organ morphology of the offspring.</p>",
"DOI": "10.5455/jabet.2023.d140",
"views": 1634,
"downloads": 240,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/15/178-1679245421-Figure1.jpg",
"caption": "Figure 1. Impact of sodium arsenite (NaAsO2) on body growth (from birth to weaning) of offspring. In control and NaAsO2 exposed mice, body growth after birth to weaning (4 weeks) of offspring from control and treatment group mice was assessed and compared according to final body weight (A), body weight gain (B), and rate of body weight gain (C). Data are presented as mean ± SEM (n=3). *p<0.002, **p<0.0001 indicates statistical significance from the control mice.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/15/178-1679245421-Figure2.jpg",
"caption": "Figure 2. Implications of gender differences in the rate of body weight gain from birth to weaning. In both control and NaAsO2 exposed mice, the rate of body weight gain from birth through weaning was assessed and compared in male and female pups. Values are presented as mean ± SEM (n=3). *p<0.005, **p<0.02 indicates statistical significance from the male offspring.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/15/178-1679245421-Figure3.jpg",
"caption": "Figure 3. Effects of Na-arsenite (NaAsO2) on different organs of F1 mice. Offspring (control and NaAsO2 exposed) after reaching sexual maturity were euthanized and organs namely the Liver (A, H), Lung (B, I), Kidney (C, J), Spleen (D, K), Heart (E, L), Uterus (F, M), and Testis (G, N) were collected and photographed. Each organ of the control and NaAsO2 exposed F1 mice were assessed based on the morphology of the organ.",
"featured": false
}
],
"authors": [
{
"id": 1005,
"affiliation": [
{
"affiliation": "Department of Genetics and Animal Breeding, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Khayrul",
"family_name": "Basher",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0009-0002-2965-5396",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 221
},
{
"id": 1006,
"affiliation": [
{
"affiliation": "Department of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
},
{
"affiliation": "School of Veterinary Medicine, Texas Tech University, 7671 Evans Drive, Amarillo, TX 79106, USA"
}
],
"first_name": "Sumon",
"family_name": "Sarkar",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-9008-1208",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 221
},
{
"id": 1007,
"affiliation": [
{
"affiliation": "Department of Genetics and Animal Breeding, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Hosne",
"family_name": "Mobarak",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0009-0002-3284-661X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 221
},
{
"id": 1008,
"affiliation": [
{
"affiliation": "Department of Genetics and Animal Breeding, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Rashedul",
"family_name": "Islam",
"email": "mrislam.biotech@hstu.ac.bd",
"author_order": 4,
"ORCID": "http://orcid.org/0000-0001-5265-2949",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Rashedul Islam, PhD; Department of Genetics and Animal Breeding, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh, e-mail: mrislam.biotech@hstu.ac.bd",
"article": 221
}
]
},
{
"id": 222,
"slug": "178-1678082901-analysis-of-staphylococcus-epidermidis-biofilm-formation-in-several-types-of-intravenous-fluids-based-on-time",
"type": "original_article",
"manuscript_id": "178-1678082901",
"published": "2023-05-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/05/178-1678082901.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 458-467.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Jannah SF, Widodo ADW, et al. Analysis of Staphylococcus epidermidis biofilm formation in\r\nseveral types of intravenous fluids based on time. J Adv Biotechnol Exp Ther. 2023; 6(2): 458-467.",
"title": "Analysis of Staphylococcus epidermidis biofilm formation in several types of intravenous fluids based on time",
"abstract": "<p>An intravenous catheter is a medical device used to inject intravenous fluid into the body. This procedure can cause coagulase-negative staphylococci (CoNS) bacteria such as <em>Staphylococcus epidermidis</em> penetrates the skin and forms biofilm on the catheter. Biofilms bring serious problems such as antibiotic resistance, the long-term effects that increase the length of staying in the hospital, cost, morbidity, and mortality. This research aimed to analyze the biofilm formation of <em>S. epidermidis </em>in several types of intravenous fluids based on time. This was a laboratory experimental research using the microtiter plate assay method and crystal violet coloring. Three 96-well microplates were inoculated with <em>S. epidermidis </em>in ringer lactate, 10% dextrose, 5% dextrose, normal saline, and gelafusal, where each plate was incubated at 3 different times of 24, 48, and 72 h, respectively. The results show that the optical density values of all intravenous fluids with bacteria within 24 and 48 h of incubation time did not show any significant differences compared to negative controls, while the 72 h treatments of 10% dextrose, 5% dextrose, and normal saline showed significant differences. This indicates that biofilms of <em>S. epidermidis </em>were not formed in intravenous fluids within 24 and 48 h of incubation time, however this bacterium started forming biofilm in 10% dextrose, 5% dextrose, and normal saline within 72 h of incubation time. In conclusion, the length of incubation time may influence biofilm formation.</p>",
"DOI": "10.5455/jabet.2023.d141",
"views": 1636,
"downloads": 409,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/15/178-1678082901-Figure1.jpg",
"caption": "Figure 1. Total biomass (OD) of S. epidermidis biofilm formation within 24 h of incubation time. According to Mann-Whitney test, all the OD values of inoculated intravenous fluids were not statistically significant compared to negative controls, but the OD value of positive control was statistically significant compared to other treatments (P < 0.05). RL, Ringer lactate; NS, normal saline.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/15/178-1678082901-Figure2.jpg",
"caption": "Figure 2. Total biomass (OD) of S. epidermidis biofilm formation within 48 h of incubation time. According to Mann-Whitney test, all the OD values of inoculated intravenous fluids were not statistically significant compared to negative controls, but the OD value of positive control was statistically significant compared to other treatments (P < 0.05). RL, Ringer lactate; NS, normal saline.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/15/178-1678082901-Figure3.jpg",
"caption": "Figure 3. Total biomass (OD) of S. epidermidis biofilm formation within 72 h of incubation time. According to Mann-Whitney test, the OD values of inoculated 10% dextrose, 5% dextrose, and NS were statistically significant compared to negative controls (P < 0.05), while the inoculated RL and gelafusal were statistically insignificant compared to negative controls. The OD value of positive control was statistically significant compared to other treatments (P < 0.05). RL, Ringer lactate; NS, normal saline.",
"featured": false
}
],
"authors": [
{
"id": 1009,
"affiliation": [
{
"affiliation": "Department of Basic Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Siti Fatkhul",
"family_name": "Jannah",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0009-0006-9602-2491",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 222
},
{
"id": 1010,
"affiliation": [
{
"affiliation": "Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Agung Dwi Wahyu",
"family_name": "Widodo",
"email": "agungimunologi@gmail.com",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-3449-768X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Agung Dwi Wahyu Widodo, Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia, e-mail: agungimunologi@gmail.com",
"article": 222
},
{
"id": 1011,
"affiliation": [
{
"affiliation": "Department of Medical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Rebekah Juniati",
"family_name": "Setiabudi",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0003-2171-8743",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 222
}
]
},
{
"id": 219,
"slug": "178-1679043419-association-of-the-changes-in-hepatic-enzymes-bilirubin-and-plasma-proteins-with-beta-thalassemia-in-iron-over-loaded-patients",
"type": "original_article",
"manuscript_id": "178-1679043419",
"published": "2023-04-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/12/178-1679043419.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 429-435.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Hussain GM, Abdullah MA, et al. Association of the changes in hepatic enzymes, bilirubin, and plasma proteins with beta-thalassemia in iron over loadedpatients. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 429-435.",
"title": "Association of the changes in hepatic enzymes, bilirubin, and plasma proteins with beta-thalassemia in iron over loaded-patients",
"abstract": "<p>Changes in liver enzymes and bilirubin in patients with thalassemia depend on genetic changes and the association of other genetic determinants. Iron overload is associated with increased morbidity in both transfusion-dependent and non-transfusion-dependent thalassemia patients. The main objective was to evaluate hepatic enzymes, bilirubin, and plasma proteins in beta-thalassemia major (βTM) patients and to study the correlations of these parameters with serum hemoglobin and ferritin concentrations in βTM patients. To achieve these, the study had two groups, the first is case group includes 39 patients with βTM and the second control group includes 34 subjects. Serum ALT, AST, total bilirubin, albumin, and total protein concentrations were measured by UV-Vis spectrophotometer, while the concentration of serum ferritin was measured by ELISA Kit. Our results showed that there were highly significant differences between βTM and ferritin, furthermore, there were highly significant differences between βTM and liver enzymes such as AST, ALT, total protein, albumin, and total bilirubin. In addition, our findings showed that there was correlation between serum ferritin concentrations and liver function parameters in βTM patients, where there are highly significant differences between βTM and ALT, while the correlation between serum hemoglobin concentration and liver function parameters showed highly significant differences between βTM and AST. The study concluded that the increase in serum liver enzymes (ALT, AST) and total bilirubin concentrations in patients with βTM are indicator to liver dysfunction that is correlated to iron overload.</p>",
"DOI": "10.5455/jabet.2023.d138",
"views": 1571,
"downloads": 216,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/42/15/178-1679043419-Figure1.jpg",
"caption": "Figure 1. Correlations of serum ferritin concentrations with liver function parameters in βTM patients.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/42/15/178-1679043419-Figure2.jpg",
"caption": "Figure 2. Correlation of serum hemoglobin concentration with liver function parameters in βTM patients.",
"featured": false
}
],
"authors": [
{
"id": 999,
"affiliation": [
{
"affiliation": "College of Pharmacy, AL-Zahraa University for Women, Karbala, Iraq"
}
],
"first_name": "Ghufran Mohammed",
"family_name": "Hussain",
"email": "ghufran.mohammed@alzahraa.edu.iq",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-8571-5421",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ghufran Mohammed Hussein, College of Pharmacy, AL-Zahraa University for Women, Karbala state, 56001, Iraq, e-mail: ghufran.mohammed@alzahraa.edu.iq",
"article": 219
},
{
"id": 1000,
"affiliation": [
{
"affiliation": "College of Pharmacy, Al-Mustaqbal University College, Babylon, Iraq"
}
],
"first_name": "Maytham Ahmed",
"family_name": "Abdullah",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 219
},
{
"id": 1001,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Al-Manara College for Medical Sciences, Amarah, Iraq"
}
],
"first_name": "Nibras Yahya",
"family_name": "Hussein",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 219
}
]
},
{
"id": 220,
"slug": "178-1679286998-phytochemicals-and-anti-hemorrhoidal-activities-of-tapak-liman-elephantopus-scaber-leaves",
"type": "original_article",
"manuscript_id": "178-1679286998",
"published": "2023-04-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/42/178-1679286998.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 436-444.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Sulistiarini R, Puranti A, et al. Phytochemicals and anti-hemorrhoidal activities of Tapak Liman (Elephantopus Scaber) leaves. J Adv Biotechnol Exp Ther. 2023; 6(2): 436-444.",
"title": "Phytochemicals and anti-hemorrhoidal activities of Tapak Liman (<span>Elephantopus Scaber</span>) leaves",
"abstract": "<p>Hemorrhoids occur due to inflammation of the rectoanal venous plexus, which causes inflammation, pain, and can cause lumps. This inflammation may cause difficulty in defecation and may even cause heavy bleeding. Empirically, Tapak Liman (<em>Elephantopus scaber </em>L.) leaves have been used to treat hemorrhoids and have been known to contain flavonoids and terpenoid lactones which are active as anti-inflammatory agents. To determine the anti-hemorrhoidal activity of <em>E. scaber</em> leaves in reducing inflammation in hemorrhoids in Wistar rats, the research begins with the extraction of <em>E. scaber</em> leaves with 70% ethanol as solvent. Extracts were analyzed for total flavonoid content with quercetin standard. Anti-hemorrhoidal activity was measured based on the degree of edema formed based on the weight of the rectum through the surgical process. Hemorrhoid was induced with croton oil for 3 days on the rectoanal area in rats. The extracts were treated at concentration of 50, 100, and 200 mg/kgW for 7 days. The drug control group used oral diclofenac sodium for 7 days. <em>E. scaber</em> leaf ethanol extract contains alkaloids, flavonoids, saponins, and polyphenols. The flavonoid level was 3.433 mgQE/g extract. This study showed that <em>E. scaber</em> leaf extract had the best anti-hemorrhoidal activity at a dose of 100 mg/kgW of extract with a significance value of p<0.05. Also, the extract of <em>E. scaber</em> leaves containing flavonoids provides a fairly good anti-hemorrhoid activity compared to the control drug diclofenac sodium. It is suggested that this plant extract might be used as a new herbal anti-hemorrhoid drug.</p>",
"DOI": "10.5455/jabet.2023.d139",
"views": 1599,
"downloads": 430,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/12/15/178-1679286998-Figure1.jpg",
"caption": "Figure 1. Standard curve for quercetin.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/12/15/178-1679286998-Figure2.jpg",
"caption": "Figure 2. The rectum has hemorrhoids after induction with croton oil on day 1 to day 3.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/12/15/178-1679286998-Figure3.jpg",
"caption": "Figure 3. Rectum with hemorrhoids: (a) External hemorrhoidal tissue; (b) Anal columns of morgagni; and (c) Internal hemorrhoidal tissue.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/12/15/178-1679286998-Figure4.jpg",
"caption": "Figure 4. Rectal weight after treating the E. scaber leaf extracts in rats for reducing edema.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/12/15/178-1679286998-Figure5.jpg",
"caption": "Figure 5. Rectoanal coefficient (RAC) in rats. * Indicates significance between control Croton oil (CO) and normal control; and # indicates that 100 mg/kgW E. scaber leaf extract has significantly anti-hemorrhoidal potential compared to 50, 100 mg/kgW extract and 5mg/KgW diclofenac sodium control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/12/15/178-1679286998-Figure6.jpg",
"caption": "Figure 6. Anti-hemorrhoidal activities of Tapak liman leaves. This study showed that E. scaber leaf extract had the best anti-hemorrhoidal activity at a dose of 100 mg/kgW.",
"featured": false
}
],
"authors": [
{
"id": 1002,
"affiliation": [
{
"affiliation": "Department of Pharmacology, Faculty of Pharmacy, Mulawarman Mulawarman University, Samarinda City, East Kalimantan, Indonesia"
}
],
"first_name": "Riski",
"family_name": "Sulistiarini",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0003-4806-9763",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 220
},
{
"id": 1003,
"affiliation": [
{
"affiliation": "Laboratory of Research and Development, Faculty of Pharmacy, Mulawarman University, Samarinda, East Kalimantan, Indonesia"
}
],
"first_name": "Anggie",
"family_name": "Puranti",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 220
},
{
"id": 1004,
"affiliation": [
{
"affiliation": "Department of Biology and Pharmacy, Faculty of Pharmacy, Mulawarman Mulawarman University, Samarinda City, East Kalimantan, Indonesia"
}
],
"first_name": "Wisnu Cahyo",
"family_name": "Prabowo",
"email": "wisnu@farmasi.unmul.ac.id",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Wisnu Cahyo Prabowo, Department of Biology and Pharmacy, Faculty of Pharmacy, Mulawarman Mulawarman\r\nUniversity, Samarinda City, East Kalimantan, Indonesia, e-mail: wisnu@farmasi.unmul.ac.id",
"article": 220
}
]
},
{
"id": 218,
"slug": "178-1675918359-antibiotic-resistance-pattern-in-the-bacterial-strains-of-urinary-tract-infection-in-tangail-city-bangladesh",
"type": "original_article",
"manuscript_id": "178-1675918359",
"published": "2023-04-21",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/01/178-1675918359.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 419-428.",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University Japan.",
"cite_info": "Akther N, Hira FA, et al. Antibiotic resistance pattern in the bacterial strains of urinary tract infection in Tangail city, Bangladesh. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 419-428.",
"title": "Antibiotic resistance pattern in the bacterial strains of urinary tract infection in Tangail city, Bangladesh",
"abstract": "<p>Antimicrobial resistance has become an alarming issue all over the world. Moreover, in the developing countries as well as in Bangladesh facing this badly due to lack of study. Antibiotic resistance is prevalent among the bacterial pathogens causing urinary tract infections. Urinary tract infections affect >30% of people around the world. Worldwide, about 150 million people experience urinary tract infections each year. Antimicrobial treatment is often prescribed ‘empirically’ without antibiotic susceptibility testing to treat urinary tract infections (UTIs). The prevalence of antibiotic resistance is worsening, probably because of the increasing number of multidrug-resistant bacterial strains and the indiscriminate use of broad-spectrum antibiotics and empirical treatment. This study aims to determine the frequency and antibiotic resistance pattern of bacteria from suspected UTIs in Bangladeshi patients. In our study, about 200 samples of positive UTIs patients were analyzed with several parameters to see the antibiotic resistance rate. After examining the patient’s sample of UTIs antibiotic resistance rate is alarming and the most causative bacteria is <em>Escherichia coli </em>which is about 61.8%. Among the 200-urine sample suspected UTI patients 66% and 30% are female and male, respectively. Empirical therapy should be guided to control the antibiotic resistance. Especially, developing country should accept the challenge with proper infrastructure to get the surveillance of resistance, as resistance varies from region to region. Appropriate antibiotics should be focused for the rational use of antibiotics to prevent antibiotics resistance through common infections.</p>",
"DOI": "10.5455/jabet.2023.d137",
"views": 2427,
"downloads": 272,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/14/178-1675918359-Figure1.jpg",
"caption": "Figure 1. Results of urine cultures with the percentage of organisms isolated.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/14/178-1675918359-Figure2.jpg",
"caption": "Figure 2. (a) Frequency and (b) percentage of patients according to gender.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/14/178-1675918359-Figure3.jpg",
"caption": "Figure 3. Graph shows the percentage of susceptibility of each antibiotic.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/14/178-1675918359-Figure4.jpg",
"caption": "Figure 4. Graph shows the percentage of resistance of each antibiotic.",
"featured": false
}
],
"authors": [
{
"id": 990,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Bangladesh"
}
],
"first_name": "Nishat",
"family_name": "Akther",
"email": "nishat31@mbstu.ac.bd",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nishat Akther, Department of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University,\r\nBangladesh, e-mail: nishat31@mbstu.ac.bd",
"article": 218
},
{
"id": 991,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Bangladesh"
}
],
"first_name": "Farjana A.",
"family_name": "Hira",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 218
},
{
"id": 992,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Bangladesh"
}
],
"first_name": "Ayesha",
"family_name": "Khatun",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 218
},
{
"id": 993,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Bangladesh"
}
],
"first_name": "Md.",
"family_name": "Shawan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 218
},
{
"id": 994,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Bangladesh"
}
],
"first_name": "Shaike M.",
"family_name": "Abdullah",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 218
},
{
"id": 995,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Bangladesh"
}
],
"first_name": "Esrat J.",
"family_name": "Shorna",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 218
},
{
"id": 996,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Bangladesh"
}
],
"first_name": "Ashekul",
"family_name": "Islam",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 218
},
{
"id": 997,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Bangladesh"
}
],
"first_name": "Khairul",
"family_name": "Islam",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 218
},
{
"id": 998,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Biological Science, University of Chittagong, Bangladesh"
}
],
"first_name": "Nazmul M.",
"family_name": "Hossain",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 218
}
]
},
{
"id": 217,
"slug": "178-1677822942-oncogenic-driver-mutations-in-vietnamese-patients-with-lung-adenocarcinoma",
"type": "short_communication",
"manuscript_id": "178-1677822942",
"published": "2023-04-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/35/178-1677822942.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 412-418.",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA",
"cite_info": "Vuong LD, Nguyen QN. Oncogenic driver mutations in Vietnamese patients with lung adenocarcinoma. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 412-418.",
"title": "Oncogenic driver mutations in Vietnamese patients with lung adenocarcinoma",
"abstract": "<p>Mutation profiles of oncogenes play essential roles in cancer therapy, but data on the prevalence of lung cancer oncogenic driver mutations in Vietnamese patients are limited. This study aims to evaluate the mutation status of <em>EGFR, ALK, ROS1, RET,</em> and <em>MET</em> genes and analyze the association of gene mutations with clinicopathological characteristics of lung adenocarcinoma. A total of 179 tumors were collected from lung adenocarcinoma patients. The mutation frequencies of <em>EGFR, ALK, ROS1, RET</em> and <em>MET</em> genes were 44.6, 7.9, 3.0, 3.0 and 2.0%, respectively. <em>EGFR, ALK, ROS1</em> and <em>RET</em> alterations tended to be higher in females. Moreover, rearrangements of <em>ALK</em>, <em>ROS</em> and <em>RET</em> were more prevalent in younger lung adenocarcinoma but skipping at exon 14 MET was more frequent in male and older patients. Stages III and IV seem to accumulate more <em>ALK</em>, <em>RET</em> and <em>MET</em> abnormalities. These findings identified the variation of frequencies of Vietnamese lung adenocarcinoma with different clinicopathological characteristics and established the pioneer data for oncogenic diver mutation of lung cancer in Vietnam.</p>",
"DOI": "10.5455/jabet.2023.d136",
"views": 1269,
"downloads": 184,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/14/178-1677822942-Figure1.jpg",
"caption": "Figure 1. Oncogenic driver mutations in Vietnamese patients with lung adenocarcinoma. Frequency of oncogenic alterations for (A) the overall, (B) each cohort subdivided by patient characteristics such as gender, smoking status, tumor size and stages of tumors.",
"featured": false
}
],
"authors": [
{
"id": 988,
"affiliation": [
{
"affiliation": "Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam"
}
],
"first_name": "Linh Dieu",
"family_name": "Vuong",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0003-0715-0039",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 217
},
{
"id": 989,
"affiliation": [
{
"affiliation": "Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam"
}
],
"first_name": "Quang Ngoc",
"family_name": "Nguyen",
"email": "quangk8s@gmail.com",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0003-3497-1262",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Quang Ngoc Nguyen, PhD; Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam, e-mail: quangk8s@gmail.com",
"article": 217
}
]
},
{
"id": 216,
"slug": "178-1675258635-role-of-plasma-calcium-phosphorus-alkaline-phosphatase-and-parathyroid-hormone-in-the-prediction-of-vitamin-d-deficiency",
"type": "original_article",
"manuscript_id": "178-1675258635",
"published": "2023-04-18",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/14/178-1675258635.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 403-411.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Hashim RD, Nathir I, et al. Role of plasma calcium, phosphorus, alkaline phosphatase, and parathyroid hormone in the prediction of vitamin D deficiency. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 403-411.",
"title": "Role of plasma calcium, phosphorus, alkaline phosphatase, and parathyroid hormone in the prediction of vitamin D deficiency",
"abstract": "<p>Vitamin D deficiency is a highly prevalent medical condition associated with various clinical and biochemical outcomes although these outcomes might be absent in a significant percentage of patients. Altered serum calcium, phosphorus (PO4), parathyroid hormone (PTH) and alkaline phosphatase (ALP) activity are expected but not proven by various research. The current study aims to investigate the possibility of these biomarkers to be used as indicators of vitamin D deficiency or insufficiency. The study enrolled 150 randomly selected participants who have no acute or chronic medical condition with various levels of serum vitamin D ranging from normal to severely deficient. They were investigated for serum total calcium, PO4, PTH, and ALP activity. The results were statistically compared among the studied groups and correlated with serum vitamin D levels. There was no significant difference in mean serum calcium, PO4, and ALP activity among the groups with poor correlation with vitamin D. Mean serum PTH has shown a significant difference among the studied groups with a strong negative correlation with vitamin D. This difference was apparent even in patients with vitamin D insufficiency. Serum calcium, PO4 and ALP activity seem to have a poor correlation with vitamin D concentration. In addition, serum PTH might be considered a sensitive marker of both vitamin D deficiency and insufficiency.</p>",
"DOI": "10.5455/jabet.2023.d135",
"views": 1112,
"downloads": 137,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/08/178-1675258635-Figure1.jpg",
"caption": "Figure 1. The correlation between vitamin D and PTH levels.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/08/178-1675258635-Figure2.jpg",
"caption": "Figure 2. The correlation between vitamin D and serum calcium levels.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/08/178-1675258635-Figure3.jpg",
"caption": "Figure 3. The correlation between vitamin D and serum phosphorus levels.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/08/178-1675258635-Figure4.jpg",
"caption": "Figure 4. The correlation between vitamin D and serum ALP activity levels.",
"featured": false
}
],
"authors": [
{
"id": 982,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Al-Rasheed University College, Baghdad, Iraq"
}
],
"first_name": "Raid D.",
"family_name": "Hashim",
"email": "vsdasd9@gmail.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-2946-1316",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Raid D. Hashim, Department of Pharmacy, Al-Rasheed University College,\r\nBaghdad, Iraq, e-mail: vsdasd9@gmail.com",
"article": 216
},
{
"id": 983,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Al-Rasheed University College, Baghdad, Iraq"
}
],
"first_name": "Israa",
"family_name": "Nathir",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-1905-4661",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 216
},
{
"id": 984,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Al-Rasheed University College, Baghdad, Iraq"
}
],
"first_name": "Esraa",
"family_name": "Ghazy",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-7715-4484",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 216
},
{
"id": 985,
"affiliation": [
{
"affiliation": "College of Pharmacy, National University for Science and Technology, Dhi-Qar, Iraq"
}
],
"first_name": "Ihsan Al",
"family_name": "Timimi",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0001-9748-5080",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 216
},
{
"id": 986,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Al-Rasheed University College, Baghdad, Iraq"
}
],
"first_name": "Tiba M.",
"family_name": "Hameed",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-2037-9789",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 216
},
{
"id": 987,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Al-Rasheed University College, Baghdad, Iraq"
}
],
"first_name": "Mayssam Hussein Mohammed",
"family_name": "Ali",
"email": null,
"author_order": 6,
"ORCID": "http://orcid.org/0000-0003-2432-3683",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 216
}
]
},
{
"id": 213,
"slug": "178-1673069678-path-of-organotin-complexes-synthetic-factors-mechanisms-and-broad-spectrum-biological-influences",
"type": "review_article",
"manuscript_id": "178-1673069678",
"published": "2023-04-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/26/178-1673069678.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 386-402.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka 1230-Bangladesh",
"cite_info": "Kumar M, Abbas Z, et al. Path of organotin complexes: synthetic factors, mechanisms, and broad-spectrum biological influences. J Adv Biotechnol Exp Ther. 2023; 6(2): 386-402.",
"title": "Path of organotin complexes: synthetic factors, mechanisms, and broad-spectrum biological influences",
"abstract": "<p>The review of the literature demonstrated that the diverse properties of the organotin (IV) attributed to the various moieties contained inside the molecule account for the functions and utility of the organotin (IV) complexes. Furthermore, the capacity of organometallic compounds to stabilize complexes with unique stereochemistry is well documented. Due to their robust coordination chemistry, consistency, and varied molecular structures, these complexes exhibit a wide spectrum of biological activity. This article provides an overview of complexes’ arrangement and geometry, spectroscopic research, and physical, chemical, and biological properties. This review also focuses on recent developments in conventional chemistry, practical synthesis methods, and the diverse functions of organotin (IV) complexes.</p>",
"DOI": "10.5455/jabet.2023.d134",
"views": 1965,
"downloads": 205,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/11/178-1673069678-Figure1.jpg",
"caption": "Figure 1. Schematic representations of apoptotic cell death mediated by organotin complexes by upregulating and downregulating proapoptotic and antiapoptotic protein, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/11/178-1673069678-Figure2.jpg",
"caption": "Figure 2. Anti-inflammatory action of organotin complexes via inhibition of NF-κB signaling pathways.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/11/178-1673069678-Figure3.jpg",
"caption": "Figure 3. Organotin complexes as potent therapeutic agents.",
"featured": false
}
],
"authors": [
{
"id": 960,
"affiliation": [
{
"affiliation": "Department of Chemistry, Maharishi Markandeshwar University, Sadopur, Ambala-134007, India"
}
],
"first_name": "Manoj",
"family_name": "Kumar",
"email": "manojraju27@gmail.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-2062-9602",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Manoj Kumar, PhD; Department of Chemistry, Maharishi Markandeshwar University, Sadopur, Ambala 134007, India, email: manojraju27@gmail.com",
"article": 213
},
{
"id": 961,
"affiliation": [
{
"affiliation": "Department of Chemistry, Maharishi Markandeshwar University, Sadopur, Ambala-134007, India"
}
],
"first_name": "Zahoor",
"family_name": "Abbas",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
},
{
"id": 962,
"affiliation": [
{
"affiliation": "Department of Chemistry, Maharishi Markandeshwar University, Sadopur, Ambala-134007, India"
}
],
"first_name": "Priyanka",
"family_name": "Siwach",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
},
{
"id": 963,
"affiliation": [
{
"affiliation": "Department of Chemistry, Maharishi Markandeshwar University, Sadopur, Ambala-134007, India"
}
],
"first_name": "Jyotsna",
"family_name": "Sharma",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
},
{
"id": 964,
"affiliation": [
{
"affiliation": "Department of Chemistry, IEC University, Baddi, H.P., India"
}
],
"first_name": "Anita",
"family_name": "Rani",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
},
{
"id": 965,
"affiliation": [
{
"affiliation": "Department of Chemistry, Dyal Singh College, Karnal, India"
}
],
"first_name": "Shashi",
"family_name": "Sharma",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
},
{
"id": 966,
"affiliation": [
{
"affiliation": "Department of Chemistry, Pt.CLS Govt. College, Karnal, Haryana, India"
}
],
"first_name": "Pallvi",
"family_name": "Aggarwal",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
},
{
"id": 967,
"affiliation": [
{
"affiliation": "Department of Chemical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, United Arab Emirates"
},
{
"affiliation": "Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou 325035, China"
},
{
"affiliation": "Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia"
},
{
"affiliation": "Department of Sustainable Engineering, Saveetha School of Engineering, SIMATS, Chennai 602105, India"
}
],
"first_name": "Pau-Loke",
"family_name": "Show",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
},
{
"id": 968,
"affiliation": [
{
"affiliation": "Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Saudi Arabia"
},
{
"affiliation": "0Gilbert and Rose-Marie Chagoury School of Medicine, Lebanese American University, Beirut, Lebanon"
},
{
"affiliation": "Centre of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates"
}
],
"first_name": "Shafiul",
"family_name": "Haque",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
},
{
"id": 977,
"affiliation": [
{
"affiliation": "Department of Medical Lab Technology, University Institute of Applied Health Sciences, Chandigarh University, Chandigarh-140413, India"
}
],
"first_name": "Vivek Kumar",
"family_name": "Garg",
"email": null,
"author_order": 10,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
},
{
"id": 978,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Maharishi Markandeshwar Engineering College, Mullana, Ambala-133207, India"
}
],
"first_name": "Hardeep Singh",
"family_name": "Tuli",
"email": null,
"author_order": 11,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 213
}
]
},
{
"id": 207,
"slug": "178-1677649197-microrna-221-3p-promotes-cell-proliferation-migration-and-invasion-in-gastric-cancer-by-modulating-pik3r1",
"type": "original_article",
"manuscript_id": "178-1677649197",
"published": "2023-03-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1677649197.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 375-385.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Hasan M, Safaralizadeh R, et al. MicroRNA-221-3p promotes cell proliferation, migration, and invasion in gastric cancer by modulating PIK3R1. J Adv Biotechnol Exp Ther. 2023; 6(2): 375-385.",
"title": "MicroRNA-221-3p promotes cell proliferation, migration, and invasion in gastric cancer by modulating PIK3R1",
"abstract": "<p>Gastric cancer (GC), which is the fourth most prevalent cancer in the world is significantly threatened the health of people, particularly those in developing nations. Nearly all significant pathological and physiological mechanisms, including apoptosis, proliferation, cell cycle, differentiation, as well as DNA damage, are regulated by miRNAs. This study investigated the miR-221-3p expression and identified its target genes in GC tissue samples and cell lines, for an understanding of the miR-221-3p influence and basic processes in the progression of GC. GC tissues and matched marginal tissues were taken from 50 patients undergo gastric surgery. MiR-221-3p mimics, inhibitors, and negative controls (NC) were transfected into MKN-45 cells, using Lipofectamine RNAiMAX reagent. The proliferation was assessed by the MTT assay. Cell migration and invasion was assessed by Transwell assay. By combining Western blotting and qRT-PCR, the impact of miR-221-3p in the PIK3R1 expression in gastric cancer cells was examined. Overexpression of miR-221-3p significantly enhanced the migration, invasion, and proliferation of gastric cancer cells, conversely, transfection of miR-221-3p inhibitor led to opposite effect caused by overexpression of this miRNA on phenotypic characteristics of gastric cancer cell line. Additional investigation revealed that PIK3R1 was downregulated significantly by overexpression of miR-221-3p. Whereas, when the MKN-45 cells transfected with miR-221-3p inhibitor, PIK3R1 was noticeably overexpressed. Our current data indicate that miR-221-3p possibly work as a tumor promoter in the development of gastric cancer by negatively regulating PIK3R1 expression, hence miR-221-3p/ PIK3R1 highlighted as promising therapeutic targets or prognostic and diagnostic biomarkers for GC patients.</p>",
"DOI": "10.5455/jabet.2023.d133",
"views": 1327,
"downloads": 128,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/51/11/178-1677649197-Figure1.jpg",
"caption": "Figure 1. Expression of MicroRNA-221-3p (miRNA-221-3p) in human GC tissues and cell line. (A) qRT-PCR analysis of the miR-221-3p expression level showed significantly upregulated in GC tissues compared to matched marginal tissues. (B) Expressional level of miR-221-3p in MKN-45 cells post transfection, represent a significant increase in the expression of miR-221-3p in mimic group while inhibitor group showed a significant decreasing in the expression of miR-221-3p. All values are presented as mean with S.D. indicated by error bars. *p< 0.05 and **p< 0.01; compared to control group. *: significant differences; ns: no significant differences.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/51/11/178-1677649197-Figure2.jpg",
"caption": "Figure 2. miR-221-3p regulates MKN-45 cell proliferation, migration, and invasion. (A) The effect of miR-221-3p expression on the proliferation of MKN-45 cells was tested by MTT assay, cell proliferation was increased in the miRNA-221-3p upregulated MKN-45 cells, while inhibition in the cell proliferation was seen in the miRNA-221-3p downregulated MKN-45 cells. (B-C) MKN-45 cells migration and invasion were detected by Transwell assay (100×), overexpression of miRNA-221-3p enhances the migration and invasion of MKN-45 cells, whereas migration and invasion were inhibited in the miRNA-221-3p downregulated MKN-45 cells. The data are presented as the mean with. S.D. *p< 0.05 and **p< 0.01. *: significant differences; ns: no significant differences.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/51/11/178-1677649197-Figure3.jpg",
"caption": "Figure 3. PIK3R1 negatively modulated and was a target gene of miRNA-221-3p. (A) The binding sites of miRNA-221-3p to 3'-UTR of PIK3R1 mRNA; The putative hybridization between miR-221-3p and PIK3R1 3′UTR binding site (miRNA-target interactions predicted by online databases TargetScan, miRecords and miRDB). (B) PIK3R1 expression in mRNA level in the MKN-45 cells following miRNA-221-3p mimic or inhibitor transfection, MKN-45 cells transfected with miR-221-3p upregulated mimic showed a decreased in the expression of PIK3R1, while increasing in the level of PIK3R1the was noticed in MKN-45 cells transfected with miRNA-221-3p downregulated inhibitor. (C) Protein analysis for PIK3R1 measured by Western blot in the MKN-45 cells after miRNA-221-3p mimic or inhibitor transfection, PIK3R1 protein level increased in the MKN-45 cells after transfection with miRNA-221-3p downregulated inhibitor and decreased in the cells transfected with miRNA-221-3p upregulated mimic (GAPDH was used as an internal control). *p< 0.05 and **p< 0.01. *: significant differences.",
"featured": false
}
],
"authors": [
{
"id": 920,
"affiliation": [
{
"affiliation": "Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran"
}
],
"first_name": "Mohanad",
"family_name": "Hasan",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 207
},
{
"id": 921,
"affiliation": [
{
"affiliation": "Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran"
}
],
"first_name": "Reza",
"family_name": "Safaralizadeh",
"email": "safaralizadeh@tabrizu.ac.ir",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-6970-6998",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Reza Safaralizadeh, PhD; Department of Animal Biology, Faculty of Natural Sciences University of Tabriz, Tabriz, Iran, e-mail: safaralizadeh@tabrizu.ac.ir",
"article": 207
},
{
"id": 922,
"affiliation": [
{
"affiliation": "Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran"
}
],
"first_name": "Mohammad Khalaj",
"family_name": "Kondori",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 207
},
{
"id": 923,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran"
}
],
"first_name": "Saeid Latifi",
"family_name": "Navid",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 207
}
]
},
{
"id": 206,
"slug": "178-1675416867-interaction-of-epitopic-missense-variants-of-vegfa-with-therapeutic-monoclonal-antibodies",
"type": "original_article",
"manuscript_id": "178-1675416867",
"published": "2023-03-16",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/29/178-1675416867.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 359-374.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Tanim MTH, Shoily SS, et al. Interaction of epitopic missense variants of VEGFA with therapeutic monoclonal antibodies. J Adv Biotechnol Exp Ther. 2023; 6(2): 359-374.",
"title": "Interaction of epitopic missense variants of VEGFA with therapeutic monoclonal antibodies",
"abstract": "<p>Vascular Endothelial Growth Factor A (VEGFA) is a glycoprotein that mediates various biological processes, including angiogenesis, vascular permeability, and cellular migration. Aberrant VEGFA signaling is also one of the hallmarks of many types of cancer and has been implicated in various ophthalmological conditions such as diabetic macular edema and age-related macular degeneration. Consequently, a number of therapeutic monoclonal antibodies (mAb) targeting VEGFA have been developed and are widely used to treat these conditions. Bevacizumab (BVZ) and Ranibizumab (RBZ) are two such antibodies that are commercially available and used to treat various cancers and ophthalmological conditions. Nevertheless, a very high rate of non-responsiveness to these mAb treatments has been reported. Therefore, it is important to predict the response to these therapeutic mAb treatments in patients in a personalized approach. This study was aimed at analyzing the impacts of missense variants in the respective VEGFA epitopes for these two therapeutic anti-VEGFA mAbs (BVZ and RBZ) on their interaction with VEGFA through the use of multiple <em>in silico</em> tools. Three missense variants (VEGFA<sup>R82W</sup>, VEGFA<sup>R82Q</sup>, and VEGFA<sup>G92R</sup>) in VEGFA epitopes appear to significantly destabilize VEGFA-BVZ interaction, while only two variants (VEGFA<sup>R82W</sup> and VEGFA<sup>R82Q</sup>) affect the interaction of VEGFA with RBZ. The VEGFA<sup>R82W</sup> variant may be pathogenic as well. These missense variants may play roles in the observed heterogeneous response to anti-VEGFA mAb treatments in patients and, therefore, may be used as pharmacogenetic markers for the prediction of responses before administration, and thus for the improvement of therapeutic outcomes.</p>",
"DOI": "10.5455/jabet.2023.d132",
"views": 1641,
"downloads": 147,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/10/178-1675416867-Figure1.jpg",
"caption": "Figure 1. Interacting residues between VEGFA variants and bevacizumab. 2D plots of interactions between the heavy chain of bevacizumab and VEGFAWT (A), VEGFAR82W (B), VEGFAR82Q (C), VEGFAI83L (D), VEGFAG88S (E) and VEGFAG92R (F). In the 2D interaction plots, VEGFA residues are shown along the vertical axis and bevacizumab heavy chain residues are depicted along the horizontal axis. Grey- contacts/interactions (within 4 Å).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/10/178-1675416867-Figure2.jpg",
"caption": "Figure 2. Interacting residues between VEGFA variants and ranibizumab. 2D plots of interactions between the heavy chain of and VEGFAWT(A), VEGFAR82W (B), VEGFAR82Q (C), VEGFAI83L (D), VEGFAQ87R (E) and VEGFAG88S (F). In the 2D interaction plots, VEGFA residues are shown along the vertical axis and ranibizumab heavy chain residues are depicted along the horizontal axis. Grey- contacts/interactions (within 4 Å).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/10/178-1675416867-Figure3.jpg",
"caption": "Figure 3. Types of interactions between residues of VEGFA and bevacizumab heavy chain. 2D plots of interactions between VEGFA and the heavy chain of bevacizumab in VEGFAWT(A), VEGFAR82W (B), VEGFAR82Q (C), VEGFAI83L (D), VEGFAG88S (E) and VEGFAG92R (F) variants. In the 2D interaction plots, VEGFA residues are shown along the vertical axis and bevacizumab heavy chain residues are depicted along the horizontal axis. Green-hydrogen bonds; Cyan-salt bridges/ionic interactions; and Red-cation-pi interactions.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/10/178-1675416867-Figure4.jpg",
"caption": "Figure 4. Types of interactions between VEGFA and ranibizumab. 2D plots of interactions between VEGFA and the heavy chain of ranibizumab in VEGFAWT (A), VEGFAR82W (B), VEGFAR82Q (C), VEGFAI83L (D), VEGFAQ87R (E) and VEGFAG88S (F) variants. In the 2D interaction plots, VEGFA residues are shown along the vertical axis and ranibizumab heavy chain residues are depicted along the horizontal axis. Green- hydrogen bonds; and Red- cation-pi interactions.",
"featured": false
}
],
"authors": [
{
"id": 914,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Md Tamzid Hossain",
"family_name": "Tanim",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 206
},
{
"id": 915,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Sabrina Samad",
"family_name": "Shoily",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 206
},
{
"id": 916,
"affiliation": [
{
"affiliation": "Molecular Biotechnology Division, National Institute of Biotechnology, Savar, Dhaka, 1349, Bangladesh"
}
],
"first_name": "Tamim",
"family_name": "Ahsan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 206
},
{
"id": 917,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
},
{
"affiliation": "Department of Mathematics and Natural Sciences, Brac University, Dhaka 1212, Bangladesh"
}
],
"first_name": "Kaniz",
"family_name": "Fatema",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 206
},
{
"id": 918,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
},
{
"affiliation": "Department of Mathematics and Natural Sciences, Brac University, Dhaka 1212, Bangladesh"
}
],
"first_name": "Sarah Umaymah",
"family_name": "Mahdiyah",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 206
},
{
"id": 919,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh"
}
],
"first_name": "Abu Ashfaqur",
"family_name": "Sajib",
"email": "abu.sajib@du.ac.bd",
"author_order": 6,
"ORCID": "http://orcid.org/0000-0003-1710-9865",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Abu Ashfaqur Sajib, PhD; Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh, e-mail: abu.sajib@du.ac.bd",
"article": 206
}
]
},
{
"id": 198,
"slug": "178-1666227939-inhibitory-effects-of-synbiotics-on-biofilm-of-uropathogenic-escherichia-coli-during-urinary-tract-infection",
"type": "original_article",
"manuscript_id": "178-1666227939",
"published": "2023-02-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/10/178-1666227939.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 350-358.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University Bangladesh",
"cite_info": "Pertiwi MIC, Wahyunitisari MR, et al. Inhibitory effects of synbiotics on biofilm of uropathogenic Escherichia coli during urinary tract infection. J Adv Biotechnol Exp Ther. 2023; 6(2): 350-358.",
"title": "Inhibitory effects of synbiotics on biofilm of uropathogenic Escherichia coli during urinary tract infection",
"abstract": "<p>Uropathogenic <em>Escherichia coli</em> (UPEC) is a fundamental cause of nosocomial urinary tract infection (UTI). UPEC can form biofilms on the mucosa of the urinary bladder and the surface of urethral catheters, thus causing clinical problems. Therefore, the current study aimed at the potential of synbiotics to manage biofilm-associated UTIs by analyzing biofilm inhibition UPEC with cell-free supernatant (CFS) of synbiotics. Biofilm inhibition was accomplished by inoculating each microbial suspension into 96-well microplates on tryptic soy broth medium at 37°C for 48 h and a microtiter plate reader was used at 595 nm to read the OD value. The outcome (%) was calculated from the OD value of CFS-treated with UPEC. The result of this research was that each CFS of synbiotic treatment displayed significantly different (P<0.05) results and was able to inhibit UPEC biofilm. The highest percentage of biofilm inhibition of UPEC was shown in CFS 8 treatment with a value of 41.51 ± 0.687, where CFS of synbiotic from <em>Lactobacillus rhamnosus</em> bacteria with 2% inulin. The lowest percentage of biofilm inhibition UPEC was shown in CFS 1 treatment with a value of 36.56 ± 1.987, where CFS from <em>Lactobacillus plantarum</em> bacteria with 0% inulin. It could be concluded that the higher the concentration of inulin in the CFS of synbiotics, the higher the percentage value of biofilm inhibition on UPEC, which indicates the potentials to manage or prevent UPEC-induced biofilm infection.</p>",
"DOI": "10.5455/jabet.2023.d131",
"views": 1458,
"downloads": 153,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/10/178-1666227939-Figure1.jpg",
"caption": "Figure 1. Results of total biomass (OD) data for microbial treatment, control group, and CFS treatment. The OD of control group vs E. coli ATCC 25922 vs UPEC were statistically significant (*P<0.05), where the OD of UPEC isolates and the OD of ATCC 25922 E. coli are high biofilm-forming (4xODc). The OD of UPEC isolates vs CFS treatment were statistically significant (#P<0.05). The type of each treatment species is indicated on the X-axis and the treatment value OD absorbance of 595 nm is indicated on the Y-axis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/10/178-1666227939-Figure2.jpg",
"caption": "Figure 2. Percentage of biofilm inhibition. The data were compared within the treatments and considered statistically significant (P<0.05). The type of concentration inulin on the X-axis, and the treatment value (%) of biofilm inhibition on the Y-axis are indicated.",
"featured": false
}
],
"authors": [
{
"id": 870,
"affiliation": [
{
"affiliation": "Department of Basic Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Mulia I.C.",
"family_name": "Pertiwi",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-1986-4277",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 198
},
{
"id": 871,
"affiliation": [
{
"affiliation": "Department of Medical Microbiology, Clinical Microbiology Specialist Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Manik R.",
"family_name": "Wahyunitisari",
"email": "manik-r-w@fk.unair.ac.id",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Manik R. Wahyunitisari, PhD; Department of Medical Microbiology, Faculty of Medicine Universitas Airlangga, Surabaya, Indonesia, e-mail: \r\nmanik-r-w@fk.unair.ac.id",
"article": 198
},
{
"id": 872,
"affiliation": [
{
"affiliation": "Department of Medical Microbiology, Clinical Microbiology Specialist Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Rebekah J.",
"family_name": "Setiabudi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 198
},
{
"id": 873,
"affiliation": [
{
"affiliation": "Department of Medical Microbiology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia"
}
],
"first_name": "Agung D.W.",
"family_name": "Widodo",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 198
},
{
"id": 874,
"affiliation": [
{
"affiliation": "Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo Academic Hospital, Surabaya, Indonesia"
}
],
"first_name": "Nurul",
"family_name": "Wiqoyah",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 198
},
{
"id": 875,
"affiliation": [
{
"affiliation": "Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Teaching Hospital, Surabaya, Indonesia"
}
],
"first_name": "M. Vitanata",
"family_name": "Arifijanto",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 198
}
]
},
{
"id": 197,
"slug": "178-1666096922-antibacterial-and-phytochemical-effects-of-ethanol-extracts-of-syzygium-guineense-willd-dc-barks-and-mangifera-indica-l-seeds",
"type": "original_article",
"manuscript_id": "178-1666096922",
"published": "2023-01-29",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/45/178-1666096922.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 337-349.",
"academic_editor": "Akhi Moni, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Mavanza SA, Omwenga GI, et al. Antibacterial and phytochemical effects of ethanol extracts of Syzygium guineense (Willd.) DC barks and Mangifera indica L seeds. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 337-349.",
"title": "Antibacterial and phytochemical effects of ethanol extracts of Syzygium guineense (Willd.) DC barks and Mangifera indica L seeds",
"abstract": "<p>Bacterial infectious diseases account for thousands of deaths worldwide. Despite their side effects, synthetic antibiotics are currently utilized to treat bacterial infections. There has been an effort to identify alternative medicines of plant origin. Thus, the current study determined <em>in vitro</em> antibacterial activities of <em>Syzygium guineense </em>barks and seeds of <em>Mangifera indica</em> ethanol extracts, as well as their phytochemical profile<em>. </em>The tested bacteria included<em> Salmonella typhi</em>, <em>Staphylococcus aureus, Bacillus subtilis, </em>and <em>Escherichia coli. </em>Plant samples were collected from Morogoro region, Tanzania and transferred to Kenyatta University for preparation and extraction. <em>In vitro</em> antibacte<strong>r</strong>ial activities were determined by disk diffusion, MIC, and MBC methods. Selected phytochemicals of ethanol extracts were determined qualitatively. The highest antibacterial effects were observed in <em>M. indica</em> extract against <em>B. subtilis, S. aureus, E. coli </em>and<em> S. typhi</em> with zones of inhibition of 20.00 mm, 18.00 mm, 17.67 mm, and 15.67 mm, respectively. Antibacterial effects observed in <em>S. guineense</em> extract against <em>B. subtilis, S. aureus, S. typhi </em>and<em> E. coli</em> produced zones of inhibition of 15.00 mm, 14.33 mm, 10.67 mm, and 9.33 mm, respectively. The extracts showed better antibacterial effects against Gram positive bacteria than Gram negative bacteria. Qualitative phytochemical analysis of the ethanol extracts revealed alkaloids, quinones, flavonoids, phenolics, saponins, steroids, terpenoids, tannins, and glycosides. This study indicates that the ethanol extracts of the plants could be used to develop alternative remedies for treating bacterial infections. The study also suggests that the plant extracts should be subjected to <em>in vivo</em> studies.</p>",
"DOI": "10.5455/jabet.2023.d130",
"views": 1823,
"downloads": 168,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/10/178-1666096922-Figure1.jpg",
"caption": "Figure 1. Antibacterial activities of Mangifera indica against S. aureus.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/10/178-1666096922-Figure2.jpg",
"caption": "Figure 2. Comparison of in vitro antibacterial effects of tested extracts against B. subtilis. Bar graphs with a different letter in each concentration are statistically significant by one-factor ANOVA and Tukey’s HSD test (p≤0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/10/178-1666096922-Figure3.jpg",
"caption": "Figure 3. Comparison of in vitro antibacterial effects of tested extracts on S. aureus. Bar graphs with a different letter in each concentration are statistically significant by one-factor ANOVA and Tukey’s HSD test (p≤0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/10/178-1666096922-Figure4.jpg",
"caption": "Figure 4. Comparison of in vitro antibacterial effects of the tested extracts against S. typhi. Bar graphs with a different letter in each concentration are statistically significant by one-factor ANOVA and Tukey’s HSD test (p≤0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/10/178-1666096922-Figure5.jpg",
"caption": "Figure 5. Comparison of in vitro antibacterial effects of the tested extracts against E. coli. Bar graphs with a different letter in each concentration are statistically significant by one-factor ANOVA and Tukey’s HSD test (p≤0.05).",
"featured": false
}
],
"authors": [
{
"id": 861,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Kenya"
}
],
"first_name": "Spora Aloyce",
"family_name": "Mavanza",
"email": "mavanzaaloyce@students.ku.ac.ke",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-2115-862X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Spora Aloyce Mavanza, Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University,\r\nKenya, e-mail: mavanzaaloyce@students.ku.ac.ke",
"article": 197
},
{
"id": 862,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Kenya"
}
],
"first_name": "George Isanda",
"family_name": "Omwenga",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-1877-4464",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 197
},
{
"id": 863,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Kenya"
}
],
"first_name": "Mathew Piero",
"family_name": "Ngugi",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-0226-9092",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 197
}
]
},
{
"id": 195,
"slug": "178-1672850331-influence-of-the-high-mobility-group-a1-genetic-polymorphism-on-indices-of-metabolic-syndrome-and-insulin-resistance-in-the-iraqi-population-case-control-study",
"type": "original_article",
"manuscript_id": "178-1672850331",
"published": "2023-01-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/24/178-1672850331.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 326-336.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Faiq MK, Saleh ES, et al. Influence of the high mobility group A1 genetic polymorphism on indices of metabolic syndrome and insulin resistance in the Iraqi population: Case-control study. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 326-336.",
"title": "Influence of the high mobility group A1 genetic polymorphism on indices of metabolic syndrome and insulin resistance in the Iraqi population: Case-control study",
"abstract": "<p>The high mobility group A1 gene (HMGA1) rs139876191 variant has been related to metabolic syndrome and type 2 diabetes, but data are lacking in Middle Eastern populations. The study aimed to assess whether the HMGA1 rs139876191 variant is associated with metabolic syndrome risk and whether this variant predicts the risk of insulin resistance. This case-control study was carried out at single center in Kirkuk city/ Iraq from February to August 2022. Polymorphisms in HMGA1 and genotyping were identified by Sanger sequencing of genomic DNA obtained from 91 Iraqi participants (61 patients with metabolic syndrome and 30 control). Lipid profile, serum (glucose and insulin), glycated hemoglobin, blood pressure, body mass index, and waist circumference were also measured. The high prevalence of the del/del genotype of rs139876191 was found. Minor allele frequency of rs139876191 was 0.16 in both metabolic syndrome and the control group. A non-significant difference in genotyping was identified between total metabolic syndrome and the control group. The del/ins variant was associated with significantly higher waist circumference, triglycerides (TG), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), and glycated hemoglobin (HbA1c) (P=0.03, 0.041, 0.007, 0.034, and 0.001, respectively), and significantly lower high-density lipoprotein (HDL) (p=0.000). Linear regression analysis showed no significant effect of the variant (del/ins) on developing insulin resistance. Thus, rs139876191 polymorphism with del/ins genotype in the HMGA1 gene was not associated with metabolic syndrome risk but it was associated with indices of metabolic syndrome including waist circumference, TG, HDL, LDL, VLDL, and HbA1c. Besides, this variant did not predict the risk of insulin resistance.</p>",
"DOI": "10.5455/jabet.2023.d129",
"views": 1375,
"downloads": 152,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/10/178-1672850331-Figure1.jpg",
"caption": "Figure 1. Analysis of rs139876191 SNP of HMGA1 gene.",
"featured": false
}
],
"authors": [
{
"id": 858,
"affiliation": [
{
"affiliation": "Department of Clinical Pharmacy, College of Pharmacy, University of Tikrit, Salah-din, Iraq"
}
],
"first_name": "Mirna Kifah",
"family_name": "Faiq",
"email": "mirna.k.faiq@tu.edu.iq",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-6558-9309",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mirna Kifah Faiq, PhD; Department of Clinical Pharmacy, College of Pharmacy, University of Tikrit, Salah-din, Iraq, e-mail: mirna.k.faiq@tu.edu.iq",
"article": 195
},
{
"id": 859,
"affiliation": [
{
"affiliation": "Department of Clinical Laboratory Sciences, College of Pharmacy, University of Baghdad, Baghdad, Iraq"
}
],
"first_name": "Eman Saadi",
"family_name": "Saleh",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0003-1764-0058",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 195
},
{
"id": 860,
"affiliation": [
{
"affiliation": "Ministry of Health and Environment, Kirkuk Health Department, Kirkuk, Iraq"
}
],
"first_name": "Omar Basher",
"family_name": "Fathalla",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-8272-5229",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 195
}
]
},
{
"id": 192,
"slug": "178-1668119016-evaluation-of-the-inflammatory-predictive-efficiency-of-progranulin-as-compared-with-common-pro-inflammatory-regulators",
"type": "original_article",
"manuscript_id": "178-1668119016",
"published": "2023-01-12",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/28/178-1668119016.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(2): 316-325.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Mustafa NW, Naser OAS, et al. Evaluation of the inflammatory predictive efficiency of progranulin as compared with common pro-inflammatory regulators. J Adv Biotechnol Exp Ther. 2023 May; 6(2): 316-325",
"title": "Evaluation of the inflammatory predictive efficiency of progranulin as compared with common pro-inflammatory regulators",
"abstract": "<p>Progranulin (PGRN), is a multifunctional protein with profound expression in epithelial and immune cells in which plays a crucial role in controlling host-defense signaling pathways during infection and inflammation. The current study was carried out to evaluate the efficiency of PGRN as a predictive inflammatory marker for a group of diseases with different etiologies that cause acute and chronic inflammations. A total of 120 participants with various diseases including rheumatoid arthritis (RA), <em>Helicobacter pylori</em> infection, burn wound, hepatitis B, and prostate cancer, and 20 healthy people were enrolled in this study. The levels of serum PGRN and interleukin-6 (IL-6) were detected. Elevated serum PGRN levels have been reported in all patient groups when compared with those in healthy controls (<em>P</em> > 0.05). Likewise, increased serum hsCRP and IL-6 levels were seen in all patient groups. However, for some patient groups, the differences in hsCRP and IL-6 levels did not reach statistical significance (<em>P > </em>0.05) in comparison with control group. Furthermore, serum PGRN levels exhibited positive correlation with hsCRP in <em>H. pylori</em> and RA patient groups. As well as, with IL-6 only in RA patient group, whereas no significant correlations were found with the rest of studied diseases. This study concluded that PGRN is an effective nonspecific inflammatory indicator of acute and chronic inflammations. It had also a higher predictive efficiency than hsCRP and IL-6 which are commonly used as inflammatory predictive markers.</p>",
"DOI": "10.5455/jabet.2023.d128",
"views": 927,
"downloads": 126,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/07/178-1668119016-Figure1.jpg",
"caption": "Figure 1. Distribution of different patient groups and the control group according to gender. H. pylori infected patients =20; patients with hepatitis B =30; patients with RA= 20; prostate cancer patients =30; patients with burn wounds= 20 and control= 20 healthy people.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/07/178-1668119016-Figure2.jpg",
"caption": "Figure 2. Differences in mean serum progranulin levels (ng/ml) between each patient group and the control group. P-value ≤ 0.0001 significant ****.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/07/178-1668119016-Figure3.jpg",
"caption": "Figure 3. Differences in mean serum hsCRP levels (mg/dl) between each patient group and the control group. P-value ˃ 0.05 not significant (ns); P-value ≤ 0.05 significant *; P-value ≤ 0.01 significant ** and P-value ≤ 0.0001 significant ****.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/07/178-1668119016-Figure4.jpg",
"caption": "Figure 4. Differences in mean serum IL-6 levels (pg/ml) between each patient group and the control group. P-value ˃ 0.05 not significant (ns); P-value ≤ 0.05 significant * and P-value ≤ 0.001 significant ***.",
"featured": false
}
],
"authors": [
{
"id": 846,
"affiliation": [
{
"affiliation": "Electron Microscope Unit, College of Pharmacy, University of Basrah, Iraq"
}
],
"first_name": "Nisreen Waleed",
"family_name": "Mustafa",
"email": "nisrenw@yahoo.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-9477-9371",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nisreen Waleed Mustafa, PhD; Electron Microscope Unit, College of Pharmacy,University of Basrah, Iraq, e-mail: nisrenw@yahoo.com",
"article": 192
},
{
"id": 847,
"affiliation": [
{
"affiliation": "Pathological Analysis Department, College of science, University of Basrah, Iraq"
}
],
"first_name": "Ola Abdul Shaheed",
"family_name": "Naser",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 192
},
{
"id": 848,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Zaid Nabeel",
"family_name": "Elia",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 192
},
{
"id": 849,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Sanaria Fawzi",
"family_name": "Jarjes",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 192
}
]
}
]
},
{
"id": 4,
"title": "Vol6 Issue1",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2023",
"slug": "2023-vol6-issue1",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 189,
"slug": "178-1665538996-biochemical-and-in-silico-study-of-leaf-extract-from-rumex-dentatus-against-staphylococcus-aureus",
"type": "original_article",
"manuscript_id": "178-1665538996",
"published": "2022-12-21",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/17/178-1665538996.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 286-300.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Moniruzzaman M, Mostari MM, et al. Biochemical and in silico study of leaf extract from Rumex dentatus against Staphylococcus aureus. J Adv Biotechnol Exp Ther. 2023; 6(1): 286-300.",
"title": "Biochemical and in silico study of leaf extract from Rumex dentatus against Staphylococcus aureus",
"abstract": "<p><em>Rumex dentatus</em> is a medicinal plant with a variety of bioactive components that can be used to treat infections and multidrug-resistant (MDR) microbes. Infections caused by MDR microbes may pose a real threat and the available antibiotics can no longer be used to control or kill those bacteria. Thus, the study aimed to ascertain the antibacterial activity of <em>Rumex dentatus</em> leaf extract and identify a potent compound that can be used as a drug developmental agent may against <em>Staphylococcus aureus</em>. Disc diffusion method, mortality assay of brine shrimp, and DPPH free radical scavenging assay were carried out for in vitro analysis. Standard computational tools and servers such as Pymol, PyRx, and Discovery Studio were used for carrying out the in-silico studies. <em>Staphylococcus aureus</em> was isolated from the infected area of eczema sufferers and identified through morphological, biochemical, and 16SrRNA sequence analysis. <em>Rumex dentatus</em> leaf extract was diluted with methanol and it showed the maximum zone of inhibition (14.33 ± 0.68 mm) after applying it against <em>Staphylococcus aureus</em> at the dose of 150µg/disc. Furthermore, the extract revealed remarkable antioxidant activity and the death rate of brine shrimp and leaf extract concentration were positively connected. Finding pharmacological targets with a high affinity for the FosB (4nb2) protein, which controls antibiotic resistance in <em>Staphylococcus aureus</em>, was done via molecular docking. 1-alpha-18O-1,25-dihydroxycholecalciferol (CID-5280453), androstan-3-ol, 9-methyl-(3 beta,5 alpha) (CID-22215820), phenylacetaldehyde (CID-998), and benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy- methyl ester (CID-62603) substances had binding energies of -6.8 Kcal/mol, -6.6 Kcal/mol, -5.6 kcal/mol, and -5.5 kcal/mol, respectively. As an organic source of medicines, <em>Rumex dentatus</em> leaf extract could be used to combat the antimicrobial-resistant pathogen.</p>",
"DOI": "10.5455/jabet.2023.d126",
"views": 1488,
"downloads": 187,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/17/178-1665538996-Figure1.jpg",
"caption": "Figure 1. Molecular identification of the isolate. Here, A) describes the profile of 27F and 1492R generated 16SrRNA from the isolate, M: denotes 1kb DNA ladder (marker). B) indicates the phylogenetic position of Staphylococcus aureus. Staphylococcus aureus produced 99% identity with the 16S rRNA gene sequencing of the isolated bacterium.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/17/178-1665538996-Figure2.jpg",
"caption": "Figure 2. A) Antibacterial activity of Rumex dentatus leaf extract showed 12.52 ± 0.48 mm, 13.12 ± 0.53 mm, 14.33 ± 0.68 mm, 14.18 ± 0.54 mm inhibition zones against Staphylococcus aureus at 50, 100, 150 and 200µg/disc, respectively. B) Different letters indicate significant differences between mean ± SD of replications (n = 3) at a p < 0.05 significance level.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/17/178-1665538996-Figure3.jpg",
"caption": "Figure 3. Cytotoxic activity of Rumex dentatus plant leaves extract against brine shrimp. These graphs demonstrate a positive connection between leaf extract concentration and brine shrimp mortality, with a high mortality percentage at 200 µg/mL, especially in comparison to other recommended doses, and a low survival percentage at 100 µg/mL. Different letters indicate significant differences between mean ± SD of replications (n = 3) at a p < 0.05 significance level.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/17/178-1665538996-Figure4.jpg",
"caption": "Figure 4. Antioxidant activity of Rumex dentatus plant leaves extract. A) DPPH scavenging activity leaf extract from Rumex dentatus, and B) IC50 value of leaf extract from Rumex dentatus. Different letters indicate significant differences between mean ± SD of replications (n = 3) at a p < 0.05 significance level.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/17/178-1665538996-Figure5.jpg",
"caption": "Figure 5. Antibiotic sensitivity test of the isolate, where A) indicates that the isolate was susceptible to gentamycin and ciprofloxacin, and resistant to amoxicillin, cefuroxime and fosfomycin, and B) indicates that the inhibitory activity of five antibiotics against isolate. Different letters indicate significant differences between mean ± SD of replications (n = 3) at a p < 0.05 significance level.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/17/178-1665538996-Figure6.jpg",
"caption": "Figure 6. Molecular docking of the protein FosB (4nb2) and plant chemicals. Here, A, B, and C represent the Cartoon view, 2D view, and surface view of the CID-5280453 and FosB (4nb2) protein complex, respectively, and D, E, and F represent the Cartoon view, 2D view, and surface view of the CID-22215820 and FosB (4nb2) protein complex.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/17/178-1665538996-Figure7.jpg",
"caption": "Figure 7. Molecular docking of the protein FosB (4nb2) and plant chemicals. A, B, and C represent the Cartoon view, 2D view, and surface view of the CID- 998 and FosB (4nb2) protein complex, respectively, and D, E, and F represent the Cartoon view, 2D view, and surface view of the CID- 62603 and FosB (4nb2) protein complex.",
"featured": false
}
],
"authors": [
{
"id": 822,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Md.",
"family_name": "Moniruzzaman",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 189
},
{
"id": 823,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Mst. Merina",
"family_name": "Mostari",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 189
},
{
"id": 824,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Shirmin",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 189
},
{
"id": 825,
"affiliation": [
{
"affiliation": "Department of Mathematics and Natural Sciences, BRAC University, Dhaka-1212, Bangladesh"
}
],
"first_name": "Mst. Maskera",
"family_name": "Jinnah",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 189
},
{
"id": 826,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Jui",
"family_name": "Biswas",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 189
},
{
"id": 827,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Suvro",
"family_name": "Biswas",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 189
},
{
"id": 828,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Md. Ariful",
"family_name": "Islam",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 189
},
{
"id": 829,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Shahriar",
"family_name": "Zaman",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 189
},
{
"id": 830,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Md. Abu",
"family_name": "Saleh",
"email": "saleh@ru.ac.bd",
"author_order": 9,
"ORCID": "http://orcid.org/0000-0001-5610-4763",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Abu Saleh, Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh,\r\nemail: saleh@ru.ac.bd",
"article": 189
},
{
"id": 831,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Md. Salah",
"family_name": "Uddin",
"email": "salim.geb@ru.ac.bd",
"author_order": 10,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Salah Uddin, Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh, email: salim.geb@ru.ac.bd",
"article": 189
}
]
},
{
"id": 191,
"slug": "178-1666268056-cardiocurative-effects-of-aqueous-leaf-extracts-of-ximenia-americana-linn-and-pappea-capensis-eckl-and-zeyh-against-myocardial-infarction-in-rats",
"type": "original_article",
"manuscript_id": "178-1666268056",
"published": "2022-12-21",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/04/178-1666268056.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 301-315.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Gaichu DM, Mathabe P, et al. Cardiocurative effects of aqueous leaf extracts of Ximenia americana (linn.) and Pappea capensis (eckl. and zeyh.) against myocardial infarction in rats. J Adv Biotechnol Exp Ther. 2023; 6(1): 301-315.",
"title": "Cardiocurative effects of aqueous leaf extracts of Ximenia americana (linn.) and Pappea capensis (eckl. and zeyh.) against myocardial infarction in rats",
"abstract": "<p>Different plants are traditionally applied in the treatment of different diseases, including chest pains, hypertension, and inflammation, among others. This study scientifically evaluated the curative treatment effects of <em>X. americana</em> and <em>P. capensis</em> leaf aqueous extracts in rats induced with myocardial infarction. The rats were randomly distributed as follows: Normal control group, consisting of rats treated with oral normal saline; Negative control group, comprising rats induced with MI and treated with normal saline; Positive control group, consisting of rats induced with MI and orally treated with propranolol (10 mg/Kg bw); and three experimental groups consisting of rats induced with MI and treated with plant extracts at dose levels 150, 200 and 250 mg/Kg bw. This study’s findings demonstrated that there was a significant reduction in the amounts of cTnT, LDH-1, CK-MB, T-cholesterol, triglyceride, LDL-c, and malondialdehyde, as well as a significant increase in levels of HDL-c, catalase, SOD, and glutathione peroxidase among rats treated with propranolol and plant extracts as compared to the negative control rats. Additionally, LC-MS analysis revealed the presence of flavonoids, tannins, and phenolics, which could explain the observed cardiocurative effects. This study concluded that aqueous leaf extracts of <em>X. americana </em>and <em>P. capensis </em>possess cardiocurative activities in rats with myocardial infarction. However, further studies on isolated fractions of the phytochemicals present are recommended. This study provides preliminary data useful in the development of pure and safe cardioactive compounds.</p>",
"DOI": "10.5455/jabet.2023.d127",
"views": 1172,
"downloads": 113,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/17/178-1666268056-Figure1.jpg",
"caption": "Figure 1. Comparison of effects between X. americana and P. capensis extracts on relative heart weight in rats induced with myocardial infarction. Bars with unique alphabet at each treatment are significantly distinct (p ≤ 0.05). effects of the two plant extracts were comparable at each extract dose level.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/17/178-1666268056-Figure2.jpg",
"caption": "Figure 2. Comparison of effects between X. americana and P. capensis extracts on serum cTnT, CK-MB and LDH levels in rats induced with myocardial infarction. Pappea capensis extracts showed significantly greater reduction on levels of (A) cTnT, (B) CK-MB and (C) LDH as compared with X. americana extracts. Bars with unique alphabet at each treatment are significantly distinct (p ≤ 0.05). cTnT = Cardiac troponin T; CK-MB = Creatine kinase MB; LDH.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/17/178-1666268056-Figure3.jpg",
"caption": "Figure 3. Comparison of effects between X. americana and P. capensis extracts on T-Chol, TG, LDL and HDL levels in rats Induced with myocardial infarction. A) P. capensis extract dose level 150 mg/Kg bw had significantly greater effects on T-Chol levels as compared with corresponding X. americana extract dose level. B) Effects of the two extracts on TG were similar. C) Effects of the two extracts on LDL were similar. D) P. capensis caused greater increase in levels of HDL at dose levels 150 and 250 mg/Kg bw as compared to corresponding X. americana extract dose levels. Bars with unique alphabet at each treatment are significantly distinct (p ≤ 0.05). T-Chol = Total cholesterol; TG = Triglycerides; LDL = Low-density lipoproteins; HDL = High-density lipoproteins; mg/dL = Milligram per deciliter.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/17/178-1666268056-Figure4.jpg",
"caption": "Figure 4. Comparison of effects between X. americana and P. capensis extracts on SOD, CAT, GPx and MDA levels in rats Induced with myocardial infarction. A) At dose level of 250 mg/Kg bw, P. capensis extract caused higher increase in SOD levels as compared to corresponding X. americana extract. B) Effects of the two extracts on CAT levels were similar. C) P. capensis extract caused significantly higher increase in GPx levels at the three extract dose levels. D) At dose level 150 mg/Kg bw, P. capensis extract caused greater reduction in MDA levels as compared with X. americana extract. Bars with unique alphabet at each treatment are significantly distinct (p ≤ 0.05). SOD = Superoxide dismutase; CAT = Catalase; GPx = Glutathione peroxidase; MDA = Malondialdehyde; U/g = Units per gram",
"featured": false
}
],
"authors": [
{
"id": 843,
"affiliation": [
{
"affiliation": "Department of Physical Sciences, Chuka University, 109-60400, Chuka, Kenya"
},
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, 43844-00100, Nairobi, Kenya"
}
],
"first_name": "Daniel Muthee",
"family_name": "Gaichu",
"email": "dgaichu@chuka.ac.ke",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Daniel Muthee Gaichu, Department of Physical Sciences Chuka University, Chuka, Kenya, email: dgaichu@chuka.ac.ke",
"article": 191
},
{
"id": 844,
"affiliation": [
{
"affiliation": "School of Agriculture, Food and Environment, Royal Agricultural University, GL7 6SJ, Cirincester, United Kingdom"
}
],
"first_name": "Patricia",
"family_name": "Mathabe",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 191
},
{
"id": 845,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, 43844-00100, Nairobi, Kenya"
}
],
"first_name": "Mathew Piero",
"family_name": "Ngugi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 191
}
]
},
{
"id": 188,
"slug": "178-1664789774-accessing-the-prevalence-of-cancer-biomarkers-in-suspected-patients-from-northeastern-part-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1664789774",
"published": "2022-12-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1664789774.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 276-285.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Hasnat MA, Mia MW, et al. Accessing the prevalence of cancer biomarkers in suspected patients from northeastern part of Bangladesh. J Adv Biotechnol Exp Ther. 2023; 6(1): 276-285.",
"title": "Accessing the prevalence of cancer biomarkers in suspected patients from northeastern part of Bangladesh",
"abstract": "<p>Early detection and/or characterization of cancer-associated biomarkers have revolutionized the personalized treatment approach of cancer to date. Therefore, this study was designed to access the prevalence of suspected cancer biomarkers from northeastern part of Bangladesh. A total of 892 patients’ data were analyzed in a cross-sectional manner and the collection period was 2017 to 2019. An ELISA-based chemiluminescent microparticle immune assay was used to detect serum cancer biomarkers. An unpaired t-test was used to compare the independent variables. Data were clustered into four categories: group1 (10-17 yr, adolescent), group2 (18 to 35 yr, young adult), group3 (36 to 55 yr, middle), and group4 (>55 yr, older adult). Serum prostate-specific antigen (PSA) level was higher in group4 than group3 (8.422 ± 1.423 vs 3.884 ±1.15 ng/ml). Cancer antigen (CA)-125 is significantly higher in females with age > 35 yr (older and middle) than at age <35 yr (young adult and adolescent). No significant difference in AFP was found among the four age groups while sexual disparity has shown a statistically significant difference. Both males and females from group4 were showing a higher expression level of carcinoembryonic antigen (CEA) (96.94 ± 54.02 and 48.23 ± 25.45 ng/ml, respectively) though gender-wise test frequency was same. CA19-9 was high in group4 whereas no correlation was found between the gender variation. Thus, the study has shown that level of PSA, CA-125, CEA and CA19-9 were upregulated in older peoples, while AFP was higher in male than female.</p>",
"DOI": "10.5455/jabet.2023.d125",
"views": 1231,
"downloads": 191,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/17/178-1664789774-Figure1.jpg",
"caption": "Figure 1. A) Frequency of studied six biomarkers i.e., PSA, CA125, CA15-3, AFP, CEA, and CA19-9. B) percentage of male and female patients. C) distribution percentage of the respective cancer biomarkers within the gender. Data represent mean±sem.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/17/178-1664789774-Figure2.jpg",
"caption": "Figure 2. The expression level of serum PSA is higher in adult older group4 than in middle age group3, though it is not statistically significant (p< 0.05). ns, not significant, using a two-tailed unpaired student’s t-test. Data represent mean±sem.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/17/178-1664789774-Figure3.jpg",
"caption": "Figure 3. CA-125 level among the four groups of suspected female patients. Significantly high in middle and older age female groups 3 and 4. *P<0.05, ****P<0.0001, ns, not significant, using two-tailed unpaired student’s t-test. Data represent mean±sem.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/17/178-1664789774-Figure4.jpg",
"caption": "Figure 4. CA15-3 expression level between group2 and 4 of suspected female patients. ns, not significant, using a two-tailed unpaired student’s t-test. Data represent mean±sem.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/17/178-1664789774-Figure5.jpg",
"caption": "Figure 5. A) AFP expression level among the group2, 3, and 4 of suspected patients having both genders. B) irrespective of any group, male patients expressed higher AFP than female patients, which was statistically significant. **P<0.01, ns, not significant, using a two-tailed unpaired student’s t-test. Data represent mean±sem.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/17/178-1664789774-Figure6.jpg",
"caption": "Figure 6. The expression level of CEA from suspected patients of male and female of groups 2, 3, and 4. ns, is not significant, using a two-tailed unpaired student’s t-test. Data represent mean±sem.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/20/17/178-1664789774-Figure7.jpg",
"caption": "Figure 7. A) CA19-9 expression level among the group2, 3, and 4 of suspected patients. B) The same level of CA19-9 was expressed between male and female patients. C) No significant difference was found in gender variation in any age group of patients. ns, not significant, using a two-tailed unpaired student’s t-test. Data represent mean±sem.",
"featured": false
}
],
"authors": [
{
"id": 816,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Mohammad Abul",
"family_name": "Hasnat",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-8412-5649",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 188
},
{
"id": 817,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Md. Waseque",
"family_name": "Mia",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 188
},
{
"id": 818,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Saifuddin",
"family_name": "Sarker",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 188
},
{
"id": 819,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Md. Belal",
"family_name": "Chowdhury",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 188
},
{
"id": 820,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Musaddik Ahmed",
"family_name": "Chowdhury",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 188
},
{
"id": 821,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Zafrul",
"family_name": "Hasan",
"email": "zafrul-bmb@sust.edu",
"author_order": 6,
"ORCID": "http://orcid.org/0000-0001-5914-8411",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Zafrul Hasan, PhD; Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114,\r\nBangladesh, e-mail: zafrul-bmb@sust.edu",
"article": 188
}
]
},
{
"id": 183,
"slug": "178-1666931377-effects-of-urea-molasses-straw-on-growth-performance-and-hemato-biochemical-parameters-in-sahiwal-steers",
"type": "original_article",
"manuscript_id": "178-1666931377",
"published": "2022-12-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/12/178-1666931377.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 265-275.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Islam S, Bhuiyan MEJ, et al. Effects of urea molasses straw on growth performance and hemato-biochemical parameters in Sahiwal steers. J Adv Biotechnol Exp Ther. 2023; 6(1): 265-275.",
"title": "Effects of urea molasses straw on growth performance and hemato-biochemical parameters in Sahiwal steers",
"abstract": "<p>At relatively low concentrations in the diet, molasses is extensively used as a vital energy supplement to improve growth, meat quality, and general health condition. However, there is little information on feeding molasses to steers: that’s why the study sought to determine the effects of urea molasses supplementation on growth performance and hemato-biochemical parameters. Twenty Sahiwal steers between 1.5 and 2 years old were divided into two groups (n=10); steers of group A received untreated straw (Control), while steers of group B received urea molasses straw (UMS). The body weight of the steers was measured at every 15 days intervals, and after 90 days of the experimental period blood samples were collected and serum was prepared for hemato-biochemical analysis. The result revealed that body weight of the UMS-treated steers increased significantly from day 15 to day 90 of the experiment compared to the untreated control. UMS induced a significant increase in hematological parameters such as TEC (<em>p</em><0.01), Hb (<em>p</em><0.01), and PCV (<em>p</em><0.05), whereas there were no alterations in TLC and DLC values. Further, UMS treated group showed no significant changes in biochemical parameters such as AST, ALT, and lipid profile (TC, TG, HDL, LDL) while urea and creatinine levels increased significantly (<em>p</em><0.05). Therefore, it could be suggested that UMS had beneficial effects on growth performance and hemato-biochemical parameters in Sahiwal steers.</p>",
"DOI": "10.5455/jabet.2023.d124",
"views": 1345,
"downloads": 197,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/17/178-1666931377-Figure1.jpg",
"caption": "Figure 1. Effect of UMS feeding on body weight. The mean body weights at day 1 to day 90 are presented in the Figure. Group A- Control (Normal straw), and Group B- UMS treated group. All data were expressed as mean ± SD of 10 animals in each group. Unpaired t-test was done to compare the results between the two groups. Here, ***=Statistically significant at 0.1% level (p<0.001), **=Statistically significant at 1% level (p<0.01), *= Statistically significant at 5% level (p<0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/17/178-1666931377-Figure2.jpg",
"caption": "Figure 2. Effect of UMS feeding on A) TEC, B) Hb, and C) PCV. The mean values of TEC, Hb, and PCV are presented in the Figure. Group A- Control (Normal straw), and Group B- UMS treated group. All data were expressed as mean ± SD of 10 animals in each group. Unpaired t-test was done to compare the results between the two groups. Here, **=Statistically significant at 1% level (p<0.01), *= Statistically significant at 5% level (p<0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/17/178-1666931377-Figure3.jpg",
"caption": "Figure 3. Effect of UMS feeding on TLC. The mean values of TLC are presented in the Figure. Group A- Control (Normal straw), and Group B- UMS treated group. All data were expressed as mean ± SD of 10 animals in each group. Unpaired t-test was done to compare the results between the two groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/17/178-1666931377-Figure4.jpg",
"caption": "Figure 4. Effect of UMS feeding on DLC. The mean values of A) neutrophil, B) eosinophil, C) basophil, D) lymphocyte, and E) monocyte are presented. Group A- Control (Normal straw), and Group B- UMS treated group. All data were expressed as mean ± SD of 10 animals in each group. Unpaired t-test was done to compare the results between the two groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/17/178-1666931377-Figure5.jpg",
"caption": "Figure 5. Effect of UMS feeding on liver function. The mean values of A) AST and B) ALT are presented in the graph. Group A- Control (Normal straw), and Group B- UMS treated group. All data were expressed as mean ± SD of 10 animals in each Figure. Unpaired t-test was done to compare the results between the two groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/17/178-1666931377-Figure6.jpg",
"caption": "Figure 6. Effect of UMS feeding on kidney function. The mean values of A) urea and B) creatinine are presented in the Figure. Group A- Control (Normal straw), and Group B- UMS treated group. All data were expressed as mean ± SD of 10 animals in each group. Unpaired t-test was done to compare the results between the two groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/17/178-1666931377-Figure7.jpg",
"caption": "Figure 7. Effect of UMS feeding on lipid profile. The mean values of A) TC, B) TG, C) HDL, and D) LDL are presented in the Figure. Group A- Control (Normal straw), and Group B- UMS treated group. All data were expressed as mean ± SD of 10 animals in each group. Unpaired t-test was done to compare the results between the two groups.",
"featured": false
}
],
"authors": [
{
"id": 779,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sumaiya",
"family_name": "Islam",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-7907-1068",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 183
},
{
"id": 780,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Eftakhar Jahan",
"family_name": "Bhuiyan",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0003-2567-3685",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 183
},
{
"id": 781,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Golzar",
"family_name": "Hossain",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-1487-5444",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 183
},
{
"id": 782,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Eliusur Rahman",
"family_name": "Bhuiyan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 183
},
{
"id": 783,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sharmin",
"family_name": "Akter",
"email": "sharmin.akter@bau.edu.bd",
"author_order": 5,
"ORCID": "http://orcid.org/0000-0001-6901-3377",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sharmin Akter, PhD; Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, e-mail: sharmin.akter@bau.edu.bd",
"article": 183
}
]
},
{
"id": 181,
"slug": "178-1665026899-effect-of-piper-crocatum-leaves-extract-on-atherosclerosis-in-diabetic-rats",
"type": "original_article",
"manuscript_id": "178-1665026899",
"published": "2022-12-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/54/178-1665026899.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 254-264.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Adiansyah MW, Mahati E, et al. Effect of Piper crocatum leaves extract on atherosclerosis in diabetic rats. J Adv Biotechnol Exp Ther. 2023; 6(1): 254-264.",
"title": "Effect of Piper crocatum leaves extract on atherosclerosis in diabetic rats",
"abstract": "<p>Atherosclerosis is caused by an inflammatory process in the endothelium due to an imbalance between oxidant and antioxidant agents that often occurs in Diabetes mellitus (DM) patients. Antioxidants that function to neutralize superoxide can be used to prevent atherosclerosis in DM. <em>Piper crocatum</em> leaves shows an unknown antioxidant effect on the atherosclerosis process. The aim of this study was to determine the effect <em>Piper crocatum </em>leaves extract on the severity of atherosclerosis in diabetic rats. Thirty Sprague Dawley rats were randomly distributed into 6 groups such as HC (healthy control), NC (diabetic-no therapy), PC (metformin 45 mg/kg), PC200, PC300, and PC400 (<em>Piper crocatum</em> leaves extract 200, 300, and 400 mg/kg/day). Blood sampling and histopathological analysis were performed after 14 days of treatment. MDA was measured using the TBARS method. The severity of atherosclerosis of the rat carotid artery was observed from tissue stained with haematoxylin eosin (HE) staining. Dose dependent treatment of <em>Piper crocatum</em> leaves extract decreased the blood glucose and MDA levels in the PC200, PC300, and PC400 groups compared to group NC while the body weight was increased. The study showed a decrease in the severity of atherosclerosis in groups PC200, PC300, PC400 compared to groups NC. In conclusion, <em>Piper crocatum</em> leaves extract may influence in decreasing blood glucose, MDA, and severity of atherosclerosis in diabetic rats.</p>",
"DOI": "10.5455/jabet.2023.d123",
"views": 1357,
"downloads": 239,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/17/178-1665026899-Figure1.jpg",
"caption": "Figure 1. Effect of Piper crocatum leaves extract on body weight in rats. Results from post-hoc LSD test are indicated on the graph in case significant body weight differences were found. HC (nondiabetic), NC (diabetic-no therapy), PC (metformin 45 mg/kg), PC200, PC300, and PC400 (Piper crocatum leaves extract 200/300/400 mg/kg). * Significant (p < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/17/178-1665026899-Figure2.jpg",
"caption": "Figure 2. Effect of Piper crocatum leaves extract on blood glucose levels in rats. Results from post-hoc Mann Whitney test are indicated on the graph in case significant blood glucose differences were found. HC (nondiabetic), NC (diabetic-no therapy), PC (metformin 45 mg/kg), PC200, PC300, and PC400 (Piper crocatum leaves extract 200/300/400 mg/kg). * Significant (p < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/17/178-1665026899-Figure3.jpg",
"caption": "Figure 3. Effect of Piper crocatum leaves extract on blood MDA levels in rats. Results from post-hoc Games Howell test are indicated on the graph in case significant blood MDA differences were found. HC (nondiabetic), NC (diabetic-no therapy), PC (metformin 45 mg/kg), PC200, PC300, and PC400 (Piper crocatum leaves extract 200/300/400 mg/kg). * Significant (p < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/17/178-1665026899-Figure4.jpg",
"caption": "Figure 4. Effect of Piper crocatum leaves extract on carotid artery (400x magnification). Cross section of carotid artery showing lipid deposits in tunica media (red arrows), foam cell (black arrow), thrombosis (blue arrow). HC (nondiabetic), NC (diabetic-no therapy), PC (metformin 45 mg/kg), PC200, PC300, and PC400 (Piper crocatum leaves extract 200/300/400 mg/kg).",
"featured": false
}
],
"authors": [
{
"id": 767,
"affiliation": [
{
"affiliation": "Department of Biomedical Science, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Muhammad Winantyo",
"family_name": "Adiansyah",
"email": "muhammadwinantyo@gmail.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-3200-2401",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Muhammad Winantyo Adiansyah, Department of Biomedical Science, Faculty of Medicine Diponegoro University, Semarang, Central Java, Indonesia, e-mail: muhammadwinantyo@gmail.com",
"article": 181
},
{
"id": 768,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutics, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Endang",
"family_name": "Mahati",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 181
},
{
"id": 769,
"affiliation": [
{
"affiliation": "Department of Histology, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Farmaditya Eka Putra",
"family_name": "Mundhofir",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 181
},
{
"id": 770,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutics, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Nani",
"family_name": "Maharani",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 181
},
{
"id": 771,
"affiliation": [
{
"affiliation": "Department of Pharmacology and Therapeutics, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Yora",
"family_name": "Nindita",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 181
},
{
"id": 772,
"affiliation": [
{
"affiliation": "Department of Anatomical Pathology, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia"
}
],
"first_name": "Hermawan",
"family_name": "Istiadi",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 181
}
]
},
{
"id": 175,
"slug": "178-1663846091-cognitive-enhancing-properties-of-aqueous-leaf-extract-of-vigna-unguiculata-in-ketamine-induced-memory-damage-in-mice",
"type": "original_article",
"manuscript_id": "178-1663846091",
"published": "2022-12-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/59/178-1663846091.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 231-242.",
"academic_editor": "Md Nabiul Islam, PhD; Yamaguchi University , Japan",
"cite_info": "Kipkemoi DJ, Ireri AM, et al. Cognitive enhancing properties of aqueous leaf extract of Vigna unguiculata in ketamine-induced memory damage in mice. J Adv Biotechnol Exp Ther. 2023; 6(1): 231-242.",
"title": "Cognitive enhancing properties of aqueous leaf extract of Vigna unguiculata in ketamine-induced memory damage in mice",
"abstract": "<p>Conventional remedies for managing Alzheimer’s disease (AD) and related cognitive deficits are not curative but relieve the symptoms and cause adverse side effects. Alternatively, use of herbal therapies to manage cognitive illnesses has increased substantially. <em>Vigna unguiculata </em>is commonly utilized for nutritional benefits and management of neurological disorders in herbal medicine. The present study evaluated cognitive enhancing potential of <em>V. unguiculata </em>leaf aqueous extract in mice with ketamine-induced AD-like cognitive deficits. Cognitive performance indicated by step-through latency was assessed using passive avoidance test. Anti-acetylcholinesterase (anti-AChE) and antioxidant potential of the extract were determined using brains of the test animals. Further, phytochemical constituents of the extracts were determined using LC-MS. Aqueous extract of <em>V. unguiculata </em>leaf demonstrated significant prowess in combating cognitive deficits in the test animals. This was evidenced by significantly higher (p<0.001) step-through latencies in extract-treated mice than the untreated cognitively damaged mice. Moreover, cognitively impaired mice given the studied extract exhibited significantly less (p<0.001) malondialdehyde levels and AChE activity than the negative control mice. This result confirmed antioxidant and anti-AChE properties of <em>V. unguiculata, </em>indicating its potential to attenuate oxidative stress in the brain and augment cholinergic transmission. Notably, some conventional therapies for cognitive disorders, especially AD, are AChE inhibitors. The studied extract contained phytocompounds such as flavonoids and phenolics with confirmed antioxidant and anti-AChE activities, thus, its cognitive enhancing efficacy could be attributed to these phytoconstituents. Collectively, this study upholds <em>V. ungiculata</em> usefulness in management of cognitive illnesses.</p>",
"DOI": "10.5455/jabet.2023.d121",
"views": 1165,
"downloads": 169,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/29/18/178-1663846091-Figure1.jpg",
"caption": "Figure 1. Percentage inhibition of acetylcholinesterase activity by Vigna unguiculata aqueous leaf extract in the brain of mice following ketamine-induced cognitive impairment. Bars represent means±standard error of mean. Alphabets denotes significant difference between concentrations of the extract; Bars with a different superscript alphabet are significantly distinct (one-factor ANOVA followed by Fisher's LSD; p < 0.001). Asterisk (*) denotes significant difference between the negative control (ketamine treated group) and the control groups (normal and positive control); Bar with asterisks shows significant difference between the negative control (ketamine treated group) and the control groups (normal and positive control) (Un-paired t test; p < 0.001). Hash (#) denotes significant difference between the negative control (ketamine treated group) and extract treated; Bar with hash shows significant different between the negative control (ketamine treated group) and extract treated (Un-paired t test; p < 0.001).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/29/18/178-1663846091-Figure2.jpg",
"caption": "Figure 2. Effects of Vigna unguiculata aqueous leaf extract on profile of malondialdehyde (MDA) in the brain of Mice following ketamine-mediated cognitive impairment. Bars represent means±standard error of mean. Alphabets denotes significant difference between concentrations of the extract; Bars with a different superscript alphabet are significantly distinct (one-factor ANOVA followed by Fisher's LSD; p < 0.001). Asterisk (*) denotes significant difference between the negative control (ketamine treated group) and the control groups (normal and positive control); Bar with asterisks shows significant difference between the negative control (ketamine treated group) and the control groups (normal and positive control) (Un-paired t test; p < 0.001). Hash (#) denotes significant difference between the negative control (ketamine treated group) and extract treated; Bar with hash shows significant different between the negative control (ketamine treated group) and extract treated (Un-paired t test; p < 0.001).",
"featured": false
}
],
"authors": [
{
"id": 746,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Daisy Jepkosgei",
"family_name": "Kipkemoi",
"email": "jepkosgeidaisy99@gmail.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0003-3625-2755",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Daisy Jepkosgei Kipkemoi, Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied\r\nSciences, Kenyatta University, Nairobi, Kenya, e-mail: jepkosgeidaisy99@gmail.com",
"article": 175
},
{
"id": 747,
"affiliation": [
{
"affiliation": "Department of Educational Psychology, School of Education, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Anthony Murithi",
"family_name": "Ireri",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-8999-9273",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 175
},
{
"id": 748,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Mathew Piero",
"family_name": "Ngugi",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-0226-9092",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 175
}
]
},
{
"id": 14,
"slug": "178-1665555461-investigation-of-pdgf-bb-aptamer-binding-using-growth-factor-coated-particles-and-flow-cytometry",
"type": "original_article",
"manuscript_id": "178-1665555461",
"published": "2022-12-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/31/178-1665555461.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 243-253.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka 1230, Bangladesh",
"cite_info": "Sukcharoen S, Watcharapo P , et al. Investigation of PDGF-BB aptamer binding using growth factor-coated particles and flow cytometry. J Adv Biotechnol Exp Ther. 2023; 6(1): 243-253.",
"title": "Investigation of PDGF-BB aptamer binding using growth factor-coated particles and flow cytometry",
"abstract": "<p>Platelet-derived growth factor-BB (PDGF-BB), a multifunctional growth factor secreted by platelets, endothelial and cancer cells, functions as an efficient and accurate biomarker. PDGF- BB has been employed in various therapeutic applications. Tuning its bioactivity via a specific binding ligand could enhance the PDGF-BB functionality. Aptamer, a single-stranded oligonucleotide that exhibits a selective binding to PDGF-BB, is the molecule of our interest. This research aims to evaluate the PDGF-BB aptamer binding by growth factor-coated particles. PDGF-BB-coated particles were confirmed by enzyme-linked immunosorbent assay (ELISA), and aptamer binding evaluation was investigated by flow cytometry. To optimize the condition for binding evaluation, the washing cycle and concentration of PDGF-BB, polystyrene particle (PS) and aptamer were studied using flow cytometer. Based on the flow cytometry results, the concentration of PDGF-BB showed an optimal level on the number of fluorescent particles. The percentage of fluorescent particles increased when the washing cycle was reduced, and the particle concentration was decreased. The percentage of fluorescent particles also increased proportionally with the aptamer concentration. Using a flow cytometer to detect fluorescent signal from growth factor-coated particle is a promising strategy to study biomolecular binding.</p>",
"DOI": "10.5455/jabet.2023.d122",
"views": 801,
"downloads": 98,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/18/178-1665555461-Figure1.jpg",
"caption": "Figure 1. Concept for evaluation of aptamer binding. Polystrene particles (PS) are incubated with growth factor (PDGF-BB) to yield PDGF-BB-coated particles. The particles are further treated with fluorescence-labeled aptamer (PDGF-BB aptamer). Then fluorescent signals from the treated particles are measured by a flow cytometer.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/18/178-1665555461-Figure2.jpg",
"caption": "Figure 2. Unmodified PS investigated by flow cytometry A) 2D scatter plot demonstrates FSC and SSC signals from PS and B) A histogram demonstrates fluorescence signal acquired from PS. The concentrations of PS are 1) 0.25, 2) 0.125, 3) 0.0625, 4) 0.03125, 5) 0.0156, and 6) 0.0078 mg/mL.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/18/178-1665555461-Figure3.jpg",
"caption": "Figure 3. Quantified amount of PDGF-BB in freshly prepared solution (PDGF-BB) and the post incubated solution (supernatant) after the separation of PDGF-BB-coated particles.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/18/178-1665555461-Figure4.jpg",
"caption": "Figure 4. Effect of washing step on detected fluorescence intensity. A) flow cytometry histogram. A legend indicates number of washing cycle and aptamer sequences applied in each tested particle. B) Percentage of fluorescent particle. The values are presented as mean ± SD, n = 3, *P < 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/18/178-1665555461-Figure5.jpg",
"caption": "Figure 5. Effect of PDGF-BB concentration on detected fluorescence intensity. A) flow cytometry histogram. A legend indicates PDGF-BB concentration and aptamer sequences that have been used to treat the particles. B) Percentage of fluorescent particle. The values are presented as mean ± SD, n = 3, *P < 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/18/178-1665555461-Figure6.jpg",
"caption": "Figure 6. Effect of PS concentration on detected fluorescence intensity. A) flow cytometry histogram. A legend indicates the particle and aptamer sequences applied in each tested sample. B) Percentage of fluorescent particle. The values are presented as mean ± SD, n = 3, *P < 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/18/178-1665555461-Figure7.jpg",
"caption": "Figure 7. Effect of aptamer concentration on detected fluorescence intensity. A) flow cytometry histogram. A legend indicates aptamer concentration and aptamer sequences that have been used to treat the particles. B) Percentage of fluorescent particle. The values are presented as mean ± SD, n = 3, *P < 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/18/178-1665555461-Figure8.jpg",
"caption": "Figure 8. Percentage of fluorescent particles determined from PS that has been treated with designated aptamer sequences; T32, T34, T36 and T38. The values are presented as mean ± SD, n = 3, *, **P < 0.05.",
"featured": false
}
],
"authors": [
{
"id": 17,
"affiliation": [
{
"affiliation": "Head, Genetics, Advanced Research Center, Narayana Medical College, Nellore, Andhrapradesh, India"
}
],
"first_name": "Supavinee",
"family_name": "Sukcharoen",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 14
},
{
"id": 18,
"affiliation": [
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea"
}
],
"first_name": "Paphada",
"family_name": "Watcharapo",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 14
},
{
"id": 19,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science & Technology, Bangladesh"
}
],
"first_name": "Kanokkorn",
"family_name": "Vichitsakul",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 14
},
{
"id": 20,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science & Technology, Bangladesh"
}
],
"first_name": "Jiraporn",
"family_name": "Arunpanichlert",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0003-3914-213X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 14
},
{
"id": 21,
"affiliation": [
{
"affiliation": "Head, Genetics, Advanced Research Center, Narayana Medical College, Nellore, Andhrapradesh, India"
},
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Pichayanoot",
"family_name": "Rotkrua",
"email": null,
"author_order": 5,
"ORCID": "https://orcid.org/0000-0003-2672-5782",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 14
},
{
"id": 22,
"affiliation": [
{
"affiliation": "Head, Genetics, Advanced Research Center, Narayana Medical College, Nellore, Andhrapradesh, India"
},
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Boonchoy",
"family_name": "Soontornworajit",
"email": "sbooncho@tu.ac.th",
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-2680-3456",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Boonchoy Soontornworajit, Department of Chemistry, Faculty of Science and Technology,Thammasat University, Pathumthani-12120, Thailand, e-mail: sbooncho@tu.ac.th",
"article": 14
}
]
},
{
"id": 170,
"slug": "178-1661374797-knowledge-and-attitude-towards-genetically-modified-foods-a-quantitative-cross-sectional-study-among-the-educated-subjects-in-the-four-largest-divisions-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1661374797",
"published": "2022-11-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/18/178-1661374797.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 198-209.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University Bangladesh",
"cite_info": "Das A, Sumit AF. Knowledge and attitude towards genetically modified foods: A quantitative cross-sectional study among the educated subjects in the four largest divisions of Bangladesh. J Adv Biotechnol Exp Ther. 2023; 6(1): 198-209.",
"title": "Knowledge and attitude towards genetically modified foods: A quantitative cross-sectional study among the educated subjects in the four largest divisions of Bangladesh",
"abstract": "<p>The food consumers and stakeholders of Bangladesh have limited knowledge and perception over genetically modified (GM) foods. As no studies have been done regarding this among the Bangladeshi educated people on a large scale, hence this study aims to determine the level of knowledge and attitude regarding GM foods; and to explore the related factors as well. A cross-sectional survey was done with a close-ended questionnaire in the four largest divisions of Bangladesh where respondents were above 18 years of age and completed at least higher secondary level education. It was observed that among the total 614 respondents, 24.8% had no acquaintance with GM food. Of the remaining 462 respondents, 41.8% had better knowledge and 30.7% had positive attitude. The level of knowledge significantly varied with respondents’ gender, occupation, monthly income, educational status, and discipline studied where male, pharmaceutical workers, respondents with monthly income of 50001-100000 BDT, science studied respondents had significantly better knowledge than their counterparts. Similarly, respondents’ attitude on GMOs significantly differed with gender, occupation, monthly income, and education in which positive attitude were shown by respondents with better knowledge. Finally, logistic regression analysis showed that female and business studied respondents had significantly less likely to have better knowledge and positive attitude than reference group, while respondents with monthly income between 50001-100000 BDT had more likely to have better knowledge and positive attitude, and NGO workers had more likely to have positive attitude than references. Thus, this study will help the policymakers of Bangladesh to perceive the current scenario of public demands on GM foods.</p>",
"DOI": "10.5455/jabet.2023.d118",
"views": 1825,
"downloads": 190,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/18/178-1661374797-Figure1.jpg",
"caption": "Figure 1. Schematic diagram of the study methodology.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/18/178-1661374797-Figure2.jpg",
"caption": "Figure 2. Responses to questions related to knowledge and attitudes on GM food. The percentage of each response to knowledge related questions: A) and attitude related questions, and B) were shown in the bar plot. The accurate answers were marked with red color.",
"featured": false
}
],
"authors": [
{
"id": 720,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Anindya",
"family_name": "Das",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 170
},
{
"id": 721,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Ahmed Faisal",
"family_name": "Sumit",
"email": "ahmedfaisalsumit@du.ac.bd",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "Ahmed Faisal Sumit, Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh, e-mail: ahmedfaisalsumit@du.ac.bd",
"article": 170
}
]
},
{
"id": 173,
"slug": "178-1664266143-effect-of-moderate-intensity-treadmill-exercise-under-different-ambient-temperatures-on-peripheral-circulatory-responses-in-young-healthy-adults",
"type": "original_article",
"manuscript_id": "178-1664266143",
"published": "2022-11-28",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/37/178-1664266143.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 222-230.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Wada S, Mahbub MH, et al. Effect of moderate-intensity treadmill exercise under different ambient temperatures on peripheral circulatory responses in young healthy adults. J Adv Biotechnol Exp Ther. 2023; 6(1): 222-230.",
"title": "Effect of moderate-intensity treadmill exercise under different ambient temperatures on peripheral circulatory responses in young healthy adults",
"abstract": "<p>Exercise training has the potential for inducing enhancements in peripheral circulation, which can play important preventive and therapeutic roles in peripheral circulatory diseases. However, the relevant published studies show conflicting and inconclusive results. Furthermore, useful or optimum ambient temperature for this purpose has not yet been established. Therefore, we investigated the acute responses in peripheral circulation from exposure of healthy subjects to treadmill exercise under different ambient temperatures; A total of 12 young adult volunteers (males 6, females 6) randomly underwent three sessions of treadmill exercise for 30 min under three different ambient temperatures (10°C, 20°C, and 30°C), at a predetermined exercise intensity. Before and after the intervention, leg skin blood flow (SBF) was measured by laser speckle flowgraphy and hand skin temperature (ST), by digital thermometry; After the cessation of treadmill exercise, compared to the corresponding baseline values, a significant increase in SBF was observed under all ambient temperature conditions (P<0.005). During intervention, ST showed a significant decrease at 10th min of intervention under all ambient temperatures (P<0.005) with a subsequent increasing trend in it. After intervention, a significant increase in ST was observed under 20°C condition only (P<0.05). Also, after intervention, the observed increase in systolic blood pressure was less significant under 20°C condition; Treadmill exercise appears to be a useful intervention modality in inducing improvements in peripheral circulation. However, exposure to treadmill exercise at or near 20°C ambient temperature might be recommended for the purpose.</p>",
"DOI": "10.5455/jabet.2023.d120",
"views": 1508,
"downloads": 116,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/19/178-1664266143-Figure1.jpg",
"caption": "Figure 1. Boxplots displaying median with 25th and 75th percentiles for measured skin blood flow/SBF (MBR) under three different ambient temperature conditions (10C, 20C and 30C). Data were obtained before and after intervention. Levels of significant differences: ***P<0.005 versus the corresponding before/baseline values.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/19/178-1664266143-Figure2.jpg",
"caption": "Figure 2. Skin temperature/ST (°C) on the dorsal surface of the middle of left hand before, during intervention (at 10th min and 25th min), and after intervention under three ambient temperature conditions (10C, 20C and 30C). Values are shown as mean and SE (shown as error bars). Levels of significant differences: *P<0.05 and ***P<0.005 versus the corresponding before/baseline values.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/19/178-1664266143-Figure3.jpg",
"caption": "Figure 3. Boxplots displaying median with 25th and 75th percentiles for measured systolic blood pressure/SBP (mmHg) under three different ambient temperature conditions (10C, 20C and 30C). Data were obtained before and after intervention. Levels of significant differences: **P<0.01 and ***P<0.005 versus the corresponding before/baseline values.",
"featured": false
}
],
"authors": [
{
"id": 724,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Sunao",
"family_name": "Wada",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 173
},
{
"id": 725,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "MH",
"family_name": "Mahbub",
"email": "hossain@yamaguchi-u.ac.jp",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "MH Mahbub, PhD; Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-\r\n8505, Japan, e-mail: hossain@yamaguchi-u.ac.jp",
"article": 173
},
{
"id": 726,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Natsu",
"family_name": "Yamaguchi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 173
},
{
"id": 727,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Ryosuke",
"family_name": "Hase",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 173
},
{
"id": 728,
"affiliation": [
{
"affiliation": "Department of Economics, Shimonoseki City University Graduate School of Economics, Shimonoseki, Yamaguchi 751-8510, Japan"
}
],
"first_name": "Yuki",
"family_name": "Nakagami",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 173
},
{
"id": 729,
"affiliation": [
{
"affiliation": "Department of Public Health, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, Japan"
}
],
"first_name": "Hidekazu",
"family_name": "Takahashi",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 173
},
{
"id": 730,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Hiroyuki",
"family_name": "Saito",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 173
},
{
"id": 731,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Junki",
"family_name": "Shimokawa",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 173
},
{
"id": 732,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Rie",
"family_name": "Watanabe",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 173
},
{
"id": 733,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Tsuyoshi",
"family_name": "Tanabe",
"email": null,
"author_order": 10,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 173
}
]
},
{
"id": 171,
"slug": "178-1665332167-hla-b0702-class-i-allele-anti-fsh-anti-lh-and-vitamin-d3-potential-links-with-polycystic-ovary-syndrome-in-women-of-erbil-city-iraq",
"type": "original_article",
"manuscript_id": "178-1665332167",
"published": "2022-11-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/08/178-1665332167.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 210-221.",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University Bangladesh",
"cite_info": "Berwary NJA, Aziz RM. HLA-B*0702 class-I allele, anti-FSH, anti-LH, and vitamin D3: Potential links with polycystic ovary syndrome in women of Erbil city, Iraq. J Adv Biotechnol Exp Ther. 2023; 6(1): 210-221.",
"title": "HLA-B*0702 class-I allele, anti-FSH, anti-LH, and vitamin D3: Potential links with polycystic ovary syndrome in women of Erbil city, Iraq",
"abstract": "<p>Polycystic ovary syndrome (PCOS) is a widespread endocrine-reproductive-metabolic condition with severe implications for females’ health. The role of four essential parameters on PCOS, including human leukocyte antigens (HLA) represented by the HLA-B*0702 allele, anti-follicle stimulating hormone (anti-FSH) antibodies, anti-luteinizing hormone (anti-LH) antibodies, and vitamin D3 was investigated. A total of 100 samples were collected from Kurdish women attended the maternity teaching hospital and some private hospitals/laboratories in Erbil City from October 2021 to January 2022. The samples were genotyped using a PCR-based technique with specific sequence primers. The levels of anti-FSH and anti-LH antibodies were determined using an enzyme-linked immunosorbent assay (ELISA), while vitamin D3 levels were measured by an electrochemiluminescence (ECL) test on Cobas e411 immunoassay system. The odds ratio (OR) of 2.167 at a 95% confidence interval (CI) of 0.8167 to 6.330, indicated an essential link between the HLA-B*0702 allele and a risk of PCOS. Anti-FSH and anti-LH antibodies were significantly greater in PCOS patients, notably infertile women, than in healthy controls. A significant positive linear correlation was observed between antibodies against FSH and LH in patients. Most patients had hypovitaminosis D3 with a significant difference compared to controls. The results indicate that the HLA-B*0702 allele is associated with PCOS susceptibility and could be used as an immunogenetic marker. Besides, it supports the idea that anti-FSH, and anti-LH antibodies are naturally presence antibodies in PCOS patients instead of signs for autoimmunity. It also suggests that women suffer from PCOS are more prone to develop vitamin D3 deficiency.</p>",
"DOI": "10.5455/jabet.2023.d119",
"views": 1397,
"downloads": 141,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/19/178-1665332167-Figure1.jpg",
"caption": "Figure 1. An agarose gel electrophoresis (2.0%) demonstrating specific sequence primers- based typing of the HLA-B*07:02 allele using DNA samples isolated from (A) PCOS patients and (B) Healthy controls. Lane L: 100bp DNA ladder.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/19/178-1665332167-Figure2.jpg",
"caption": "Figure 2. Differences in mean serum levels between controls and PCOS patients. (A) anti-FSH antibodies using ANOVA test, (B) anti-LH antibodies using independent t-test. Mean with 95% CI bars. Controls= 28 fertile women; primary infertile PCOS patients=28; secondary infertile patients=20 women; (Note: 12 PCOS single ladies were excluded). P-value ≥ 0.05 not significant (ns); P-value 0.01 significant **, and P-value < 0.0001 significant ****.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/19/178-1665332167-Figure3.jpg",
"caption": "Figure 3. Scatter diagrams with varied Pearson’s correlations among studied parameters in PCOS patients. A) anti-FSH and anti- LH antibodies; B) anti-FSH and FSH; C) anti-LH and LH; D) anti-FSH and vitamin D3; E) anti-LH and vitamin D3.",
"featured": false
}
],
"authors": [
{
"id": 722,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Najat Jabbar Ahmed",
"family_name": "Berwary",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0003-4971-5667",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 171
},
{
"id": 723,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq"
}
],
"first_name": "Rand Maurice",
"family_name": "Aziz",
"email": "rand.aziz@epu.edu.iq",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-1104-758X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Rand Maurice Aziz, Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, Iraq, e-mail: rand.aziz@epu.edu.iq",
"article": 171
}
]
},
{
"id": 169,
"slug": "178-1662996251-niranjan-v-malini-a-antimicrobial-resistance-pattern-in-escherichia-coli-causing-urinary-tract-infection-among-inpatients-indian-j-med-res-20141396945",
"type": "original_article",
"manuscript_id": "178-1662996251",
"published": "2022-11-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/55/178-1662996251.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 184-197.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Saha SK, Haider MN, et al. Assessment of quality and shelf-life of salt-smoked cured products from Pangasius catfish at various temperatures. J Adv Biotechnol Exp Ther. 2023; 6(1): 184-197.",
"title": "Assessment of quality and shelf-life of salt-smoked cured products from Pangasius catfish at various temperatures",
"abstract": "<p>Pangasius catfish (<em>Pangasianodon hypophthalmus</em>) is popularly cultured in Bangladesh, but the farmers usually suffer financial losses due to their low market price. Development of new value-added products will enhance its popularity and acceptability to the consumers. This study was conducted to develop smoked products from pangasius catfish, to evaluate their quality and shelf-life at ambient (30-35°C) and refrigeration temperatures (4°C). Two treatments, dipped for 15 min in 10% (T1) and 15% (T2) salt solutions, and one untreated control were maintained. The smoking was conducted at 70-75°C in an improved traditional smoking kiln. The products were then sealed in polybags and stored separately at ambient and refrigeration temperatures. The sensory quality, proximate composition, salt content, total volatile base nitrogen (TVB-N) values, and viable bacterial counts were determined. The products stored at ambient temperature were rejected on day 5, 7 and 9; and those kept at refrigeration temperature, on day 28, 35, and 42, respectively for control, T1, and T2. Significant differences in proximate compositions were observed between products of different treatments, storage conditions, and storage periods. The TVB-N values were increased rapidly in control followed by the T1 samples, compared to T2 samples. Viable bacterial counts of the smoked products were gradually increased with the increasing storage periods. Considering all the assessed parameters, the T2 products were better in quality with longer shelf-life than those of control and T1. As consumers’ preference towards new fishery products has been changing, smoked products from pangasius catfish will be preferred for their taste, less bones, and higher shelf-life.</p>",
"DOI": "10.5455/jabet.2023.d117",
"views": 394,
"downloads": 131,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/19/178-1662996251-Figure1.jpg",
"caption": "Figure 1. Flow diagram showing the methodologies for preparation, quality evaluation, and shelf-life assessment of the smoked products produced from the pangasius catfish (Pangasianodon hypophthalmus).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/19/178-1662996251-Figure2.jpg",
"caption": "Figure 2. Different types of smoked products produced from Pangasius catfish; A, T1 = 10% salt treated, B, T2 = 15% salt treated, and C, control = untreated.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/19/178-1662996251-Figure3.jpg",
"caption": "Figure 3. Comparison in salt contents of the salt treated samples (T1 = 10% salt treated and T2 = 15% salt treated) smoked-cured products from pangasius catfish on different days of storage at ambient temperature (30-35°C); the error bars indicating standard deviations and an asterisk indicating significant differences (p<0.05) between the treatments.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/19/178-1662996251-Figure4.jpg",
"caption": "Figure 4. Comparison in salt contents of the salt treated samples (T1 = 10% salt treated, and T2 = 15% salt treated) smoked-cured products from pangasius catfish on different days of storage at refrigerated temperature (4°C); the error bars indicating standard deviations and an asterisk indicating significant differences (p<0.05) between the treatments.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/19/178-1662996251-Figure5.jpg",
"caption": "Figure 5. Changing pattern of TVB-N values (mg/100g) of different smoked-cured products from pangasius catfish on different days of storage at ambient temperature (30-35°C); the error bars indicating standard deviations and an asterisk indicating significant differences (p<0.05) between the control and T2.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/27/19/178-1662996251-Figure6.jpg",
"caption": "Figure 6. Changing pattern of TVB-N values (mg/100g) of different smoked-cured products from pangasius catfish on different days of storage at refrigerated temperature (4°C); the error bars indicating standard deviations, a hash mark and an asterisk indicating significant differences (p<0.05) between the control and T1, and between the control and T2, respectively.",
"featured": false
}
],
"authors": [
{
"id": 705,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
},
{
"affiliation": "Bangladesh Fisheries Research Institute, Freshwater Sub-Station, Saidpur, Nilphamari-5310, Bangladesh"
}
],
"first_name": "Srebash Kumar",
"family_name": "Saha",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 169
},
{
"id": 706,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Nurul",
"family_name": "Haider",
"email": "raselmnh@bau.edu.bd",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0001-7656-7802",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Nurul Haider, PhD; Department of Fisheries Technology Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, e-mail: raselmnh@bau.edu.bd",
"article": 169
},
{
"id": 707,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Subhash Chandra",
"family_name": "Chakraborty",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 169
}
]
},
{
"id": 167,
"slug": "178-1664048668-molecular-characterization-of-multidrug-resistant-escherichia-coli-isolated-from-human-urine-infections-with-their-antibiogram-profile",
"type": "original_article",
"manuscript_id": "178-1664048668",
"published": "2022-11-04",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/32/178-1664048668.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 172-183.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Haque MH, Islam MZ, et al. Molecular characterization of multidrug-resistant Escherichia coli isolated from human urine infections with their antibiogram profile. J Adv Biotechnol Exp Ther. 2023; 6(1): 172-183.",
"title": "Molecular characterization of multidrug-resistant Escherichia coli isolated from human urine infections with their antibiogram profile",
"abstract": "<p>Urinary tract infection (UTI) is the leading cause of hospitalization due to bacterial infection, and the frequency of multidrug-resistant <em>Escherichia coli</em> isolates from these infections is increasing worldwide. The current study aims to isolate and characterize antibiotic-resistant <em>Escherichia coli</em> and their antibiogram typing from urine samples of humans. From April to December 2019, a total of 60 human urine samples were collected aseptically and treated to primary isolation by propagation in nutrient broth followed by culture on various agar media. Gram’s staining, string techniques, biochemical characterization, PCR, and Sanger sequencing were performed to confirm <em>E. coli.</em> The Kirby-Bauer disk diffusion technique was used to test the susceptibility of all bacterial strains to thirteen typically prescribed antibiotics. The overall prevalence of <em>E. coli </em>in UTI was 66.67%. Three variations were noted in <em>E. coli</em>, all of which were single substitutions (A>T, C>T, and T>A). Phylogenetic analysis of the 16S rRNA revealed that the <em>E. coli</em> discovered in this study belonged to the genus <em>Escherichia</em> but was distinct from those identified in other countries. The antibiograms revealed that all the isolates (100%) were resistant to penicillin, ampicillin, and amoxicillin; 94.87% to doxycycline; 79.16% to gentamycin; 75.48% to ciprofloxacin; 73.07% to erythromycin; 71.66% to levofloxacin; 47.36% to ceftriaxone; and 46.66% to tetracycline. In contrast, all <em>E. coli</em> strains were sensitive to amikacin (95%), vancomycin (92.50%), and azithromycin (92.50%). People with a UTI often have multidrug-resistant <em>E. coli</em> in their urine samples, which calls for a one-health strategy to deal with this rapidly changing condition.</p>",
"DOI": "10.5455/jabet.2023.d116",
"views": 1545,
"downloads": 278,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/19/178-1664048668-Figure1.jpg",
"caption": "Figure 1. Cultural characteristics of Escherichia coli in different media and representative amplified PCR products of the 16S rRNA gene in a 1.5% Agarose gel. A) metallic sheen colonies on EMB agar; B) Red, circular, low convex, smooth, translucent, Lactose fermenters colonies on MacConkey agar; C) Alpha haemolysis on blood agar; D) Except for the water and negative control samples, the 16S rRNA gene was found in all the samples (Lane 2-5). 1 kb DNA ladder was used for comparison.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/19/178-1664048668-Figure2.jpg",
"caption": "Figure 2. Representative Sanger sequencing results of the 16S rRNA gene in E. coli. The alignment of the 16S rRNA sequence of the isolate with the sequences of representative E. coli from GenBank in the database shows different mutations.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/19/178-1664048668-Figure3.jpg",
"caption": "Figure 3. The phylogenetic tree made from the 16S ribosomal RNA gene of E. coli from different countries of the same species.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/19/178-1664048668-Figure4.jpg",
"caption": "Figure 4. Antibiotic sensitivity pattern of E. coli against thirteen different antibiotics. Thirteen different antibiotics used for disk diffusion test. All the E. coli isolates showed resistant to penicillin (100%), ampicillin (100%), and amoxicillin (100%), Doxycycline (94.87%), gentamycin (79.16%), ciprofloxacin (75.48%), erythromycin (73.07%), levofloxacin (71.66%), ceftriaxone (47.36%), and tetracycline (46.67%) whereas amikacin (95%), vancomycin (92.50%), and azithromycin (92.50%) were found to be sensitive. Statistical significance was determined by chi-square (χ2) test. *, p =0.00.",
"featured": false
}
],
"authors": [
{
"id": 700,
"affiliation": [
{
"affiliation": "Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi 6205, Bangladesh"
}
],
"first_name": "Md. Hakimul",
"family_name": "Haque",
"email": "hakim.ahvs@ru.ac.bd",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-3856-6478",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Hakimul Haque, PhD; Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi-6205, Bangladesh, e-mail: hakim.ahvs@ru.ac.bd",
"article": 167
},
{
"id": 701,
"affiliation": [
{
"affiliation": "Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi 6205, Bangladesh"
}
],
"first_name": "Md. Zohurul",
"family_name": "Islam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 167
},
{
"id": 702,
"affiliation": [
{
"affiliation": "Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi 6205, Bangladesh"
}
],
"first_name": "Md. Lovelu",
"family_name": "Miah",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 167
},
{
"id": 703,
"affiliation": [
{
"affiliation": "Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi 6205, Bangladesh"
}
],
"first_name": "Sunny Kumar",
"family_name": "Das",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 167
},
{
"id": 704,
"affiliation": [
{
"affiliation": "Department of Microbiology, Anatomy, Physiology and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe University, Bundoora, VIC 3086, Australia"
}
],
"first_name": "Subir",
"family_name": "Sarker",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-2685-8377",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 167
}
]
},
{
"id": 166,
"slug": "178-1662066505-increased-cd73-expression-is-associated-with-poorly-differentiated-gleason-score-and-tumor-size-in-prostate-cancer",
"type": "original_article",
"manuscript_id": "178-1662066505",
"published": "2022-10-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/09/178-1662066505.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 161-171.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Alghezi DA, Aljawher RQ, et al. Increased CD73 expression is associated with poorly differentiated Gleason score and tumor size in prostate cancer. J Adv Biotechnol Exp Ther. 2023; 6(1): 161-171.",
"title": "Increased CD73 expression is associated with poorly differentiated Gleason score and tumor size in prostate cancer",
"abstract": "<p>There are few prostate cancer prognostic biomarkers. However, clinical difficulties in distinguishing between aggressive and non-aggressive tumors have been observed. CD73 is a 70-kDa glycosylphosphatidylinositol-linked ecto-enzyme that reduces antitumor immunity in mouse models of tumor, particularly prostate cancer. It’s believed to be a promising biomarker for predicting the clinical development and prognosis of certain tumor types. Its function in prostate cancer, however, is unknown. This study aims to investigate the hypothesis that CD73 may be used as a biomarker in prostate cancer diagnosis and/or prognosis. Nuclear and cytoplasmic CD73 staining has been evaluated by immunohistochemistry using benign and malignant prostate tissues. The immunohistochemical study showed nuclear and cytoplasmic CD73 staining in cancerous and non-cancerous prostate tissues. Increased CD73 staining was shown in prostate cancer tissues compared to benign prostate tissues. A negative association between CD73 expression and Gleason scores has been observed. However, increased cytoplasmic CD73 staining was significantly associated with increasing tumor size. This finding suggests that CD73 may have a role in cancer development or aggressiveness, indicating that more research is needed to better understand its function and determine whether it might be used as a diagnostic biomarker for prostate cancer.</p>",
"DOI": "10.5455/jabet.2023.d115",
"views": 1067,
"downloads": 125,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/22/178-1662066505-Figure1.jpg",
"caption": "Figure 1. CD73 staining in prostate tissues. A) moderate nuclear and cytoplasmic CD73 staining (arrow) was observed in BP. B) Strong membrano-cytoplasmic staining of CD73 (arrow) was shown in PCa. C) Moderate cytoplasmic staining of CD73 (arrow) was observed in PCa. D) Weak cytoplasmic staining of CD73 (arrow) was detected in PCa. E) there was no staining (arrow) for CD73 shown in Pca. F) Weak cytoplasmic staining of CD73 (arrow) was detected in tonsil. G) Negative control shows no background staining in PCa (arrow). Pca: Prostate cancer; BP: Benign prostate; NC: Negative control. Scale bars=100 μm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/22/178-1662066505-Figure2.jpg",
"caption": "Figure 2. Two different CD73 antibodies show the expected patterns of CD37 staining prostate tissue samples. (A&C) Weak cytoplasmic CD73 (mouse monoclonal) staining (arrows) in prostate tissue. (B&D) Weak cytoplasmic CD73 (rabbit polyclonal) staining (arrows) in prostate tissue. (E and F) Negative control tissue showed negative staining (in PCa arrows). Both CD73 antibodies revealed very similar prostate tissue staining patterns. PCa: Prostate cancer; NC: Negative control. Scale bars—100 μm with inserts at 2x zoom.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/22/178-1662066505-Figure3.jpg",
"caption": "Figure 3. Cytoplasmic CD73 staining in benign and malignant prostate tissues quantified. The percentage and intensity scores for cytoplasmic IHC staining were used to quantify CD73 staining. A) Increased cytoplasmic CD73 staining significantly in PCa compared to BP tissues (p=0.0344). B) Cytoplasmic CD73 staining showed a significant difference among different Gleason scores (p=0.0072) and multiple comparison tests (Tukey) confirmed a significant reduction with increasing Gleason score. When comparing PCa patients with a high Gleason score to those with a low (p=0.0081) or intermediate (p=0.0469) Gleason score, the decrease was statistically significant. C) Cytoplasmic CD73 staining was shown to be positively associated with primary tumor volume (p=0.0144). Prostate cancer PCa (n=75) and Benign prostate BP (n=21), low Gleason score 3 (n=16), moderate Gleason score (n=29) and high Gleason score (n=22), Tumor size T1-2 (n= 44) and T3-4 (n= 30). X axis: Prostate samples: Benign prostate or Prostate cancer tissue samples. Gleason score: Low, moderate and high. T: Tumor size (T1-2 vs T3-4). Y axis: Final score (proportion and intensity) of cytoplasmic CD73 in each case.",
"featured": false
}
],
"authors": [
{
"id": 691,
"affiliation": [
{
"affiliation": "Cancer Research Unit, College of Medicine, University of Thi-Qar, Thi- Qar, Iraq"
},
{
"affiliation": "Medical Microbiology and Immunology Department, College of Medicine, University of Thi-Qar, Thi- Qar, Iraq"
}
],
"first_name": "Dhafer A.",
"family_name": "Alghezi",
"email": "dr.daf79@utq.edu.iq",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dhafer A. Alghezi, Medical Microbiology and Immunology Department,\r\nCollege of Medicine, University of Thi-Qar, Thi- Qar, Iraq, e-mail: dr.daf79@utq.edu.iq",
"article": 166
},
{
"id": 693,
"affiliation": [
{
"affiliation": "Histopathology and Forensic Medicine Department, College of Medicine, University of Thi-Qar, Thi-Qar, Iraq"
}
],
"first_name": "Rasha",
"family_name": "Aljawher",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 166
},
{
"id": 694,
"affiliation": [
{
"affiliation": "College of Dentistry, University of Thi-Qar, Thi- Qar, Iraq"
},
{
"affiliation": "College of Health and Medical technology, National University of Science and Technology, Thi- Qar, Iraq"
}
],
"first_name": "Sada Al",
"family_name": "Musawi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 166
}
]
},
{
"id": 164,
"slug": "178-1660251743-evaluation-of-oxidative-stress-activity-and-the-levels-of-homocysteine-vitamin-b12-and-dna-methylation-among-women-with-breast-cancer",
"type": "original_article",
"manuscript_id": "178-1660251743",
"published": "2022-10-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/49/178-1660251743.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 149-160.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Rubaye RHKA; Jumaily RMKA. Evaluation of oxidative stress activity and the levels of homocysteine, vitamin B12, and DNA methylation among women with breast cancer. J Adv Biotechnol Exp Ther. 2023; 6(1): 149-160.",
"title": "Evaluation of oxidative stress activity and the levels of homocysteine, vitamin B12, and DNA methylation among women with breast cancer",
"abstract": "<p>Breast cancer (BC) is the most common malignant tumor in women and the leading cause of cancer deaths worldwide. This work was conducted to estimate the roles of oxidative stress, vitamin B12, homocysteine (HCY), and DNA methylation in BC disease progression. Sixty BC patients (age range 33–80 years) and 30 healthy controls were recruited for this study. Patients with BC were split to group 1 consisted of stage II BC women (low level), and group 2 consisted of patients in stages III and IV (high level). Malondialdehyde (MDA), glutathione peroxidase 3 (GPX3), HCY, and vitamin B12 levels in the study groups were measured. Also, the 5-methylcytosine (5mC) global DNA methylation levels were evaluated. The results showed a significant increase in HCY, and MDA in BC patients compared to healthy controls, with evident increases observed in those with advanced-stage BC (stages III and IV). They were accompanied by significantly reduced levels of 5mC, with a positive correlation between 5mC and the different stages of BC. Also, patients in advanced stages and those with a poor prognosis were exposed to low levels of vitamin B12 and GPX3 (except for the patients in stage IV, which showed increased GPX3 levels). The findings of this study suggest that the differences in global DNA methylation levels at the various phases may be used as a risk factor for developing BC, which indicates the involvement of GPX3 and HCY in BC progression.</p>",
"DOI": "10.5455/jabet.2023.d114",
"views": 1497,
"downloads": 142,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/22/178-1660251743-Figure1.jpg",
"caption": "Figure 1. Standard curve for determining methylation of DNA as determined by the immunoassay. Diagrammatic representation of the linear connection between the quantity of 5-methylcytosine and its absorbance.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/22/178-1660251743-Figure2.jpg",
"caption": "Figure 2. Levels of malondialdehyde (MDA) in the blood of breast cancer patients and control subjects; A) MDA level in breast cancer patients compared to the control, B) MDA level in the early (low-level) and advanced (high-level) individuals with breast cancer in comparison to those who served as controls, ** Refers to significance at p≤0.001, Values represent the mean ± SEM. Low level consisted of patients in stage II, High level consisted of patients in stages III and IV.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/22/178-1660251743-Figure3.jpg",
"caption": "Figure 3. Levels of glutathione peroxidase (GPX3) in the blood of breast cancer patients and healthy control subjects; A) GPX3 level in breast cancer patients compared to the control, B) GPX3 level in the early (low-level) and advanced (high-level) in breast cancer patients compared to the control. * Refers to significance at p≤0.05, Values represent the mean ± SEM. Low level consisted of patients in stage II, High level consisted of patients in stages III and IV.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/22/178-1660251743-Figure4.jpg",
"caption": "Figure 4. Levels of homocysteine (HCY) in the blood of breast cancer patients and healthy control subjects; A) HCY level in breast cancer patients compared to the control, B) HCY level in the early (low-level) and advanced (high-level) in breast cancer patients compared to the control. * Refers to significance at p≤0.05, ** Refers to significance at p≤0.001, Values represent the mean ± SEM. Low level consisted of patients in stage II, High level consisted of patients in stages III and IV.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/22/178-1660251743-Figure5.jpg",
"caption": "Figure 5. Levels of vitamin B12 in the blood of breast cancer patients and healthy control subjects; A) Vitamin B12 level in breast cancer patients compared to the control, B) Vitamin B12 level in the early (low-level) and advanced (high-level) in breast cancer patients compared to the control. ** Refers to significance at p≤0.001, Values represent the mean ± SEM. Low level consisted of patients in stage II, High level consisted of patients in stages III and IV.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/22/178-1660251743-Figure6.jpg",
"caption": "Figure 6. Changes in the global contents of 5-methylcytosine (5mC) in breast cancer patients, including: A) the level of 5mC in breast cancer patients compared to the control group, and B) the level of 5mC in early (low-level) and advanced (high-level) breast cancer patients compared to the control group. * Refers to significance at p≤0.05, ** Refers to significance at p≤0.001, Values represent the mean ± SEM. Low level consisted of patients in stage II, High level consisted of patients in stages III and IV.",
"featured": false
}
],
"authors": [
{
"id": 689,
"affiliation": [
{
"affiliation": "Department of Biology, College of Education for Pure Sciences, Ibn Al-Haitham, University of Baghdad, Iraq"
}
],
"first_name": "Rana H. K.",
"family_name": "Al-Rubaye",
"email": "ranabio189@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Rana H. K. Al-Rubaye, Department of Biology, College of Education for Pure Sciences, Ibn Al-Haitham, University of Baghdad, Iraq, e-mail: ranabio189@gmail.com",
"article": 164
},
{
"id": 690,
"affiliation": [
{
"affiliation": "Department of Biology, College of science, University of Baghdad, Baghdad, Iraq."
}
],
"first_name": "Rakad M. Kh",
"family_name": "Al-Jumaily",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 164
}
]
},
{
"id": 162,
"slug": "178-1656491348-evaluation-of-phytochemical-profile-and-antimicrobial-activity-of-tragia-brevipes-extracts-against-pathogenic-bacteria",
"type": "original_article",
"manuscript_id": "178-1656491348",
"published": "2022-10-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/03/178-1656491348.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 140-148.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Migabo H, Munyeshyaka E, et al. Evaluation of phytochemical profile and antimicrobial activity of Tragia brevipes extracts against pathogenic bacteria. J Adv Biotechnol Exp Ther. 2023; 6(1): 140-148.",
"title": "Evaluation of phytochemical profile and antimicrobial activity of Tragia brevipes extracts against pathogenic bacteria",
"abstract": "<p>Infectious diseases remain a global health challenge as result of antimicrobial resistance. The use of natural products has revealed a potential source of alternative antimicrobial agents. The aim of this research was to evaluate the antibacterial activities and the phytoconstituents of <em>Tragia brevipes</em> against pathogenic bacteria. The crude extracts of leaves, roots and stems were obtained using polar and non-polar solvents and the antibacterial assay was performed using agar well diffusion and disc diffusion methods. The results showed that in the leaves and roots, flavonoids, saponins, glycosides, phenols, tannins, and resins were present whereas in the stem, flavonoids, glycosides, and resins were present. Only <em>S. aureus</em> ATCC43300, <em>E. coli</em> ATCC25922 did not show inhibition zone to all plant parts. <em>P. aeruginosa</em> ATTCC27853 showed the highest inhibition zone compared to other bacteria. It was observed that methanolic extract has a great potential antimicrobial activity, followed by distilled water extracts. The combination of plant extracts showed a marked synergic effect. The two-way ANOVA showed a statistical difference in the mean zone of inhibition obtained among bacteria and leaves (f = 2.3478, p < 0.05), roots (f = 2.3478, p < 0.05) whereas stem extracts difference was between solvents and tested bacteria (f = 3.4923, p < 0.05). <em>T. brevipes</em> could be a potential candidate in the treatment of bacterial infection as a source of novel antimicrobial agents. Further studies are recommended to isolate the active ingredients and investigate pharmacological properties of <em>T. brevipes.</em></p>",
"DOI": "10.5455/jabet.2023.d113",
"views": 1395,
"downloads": 204,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/22/178-1656491348-Figure1.jpg",
"caption": "Figure 1. Antimicrobial activity of T. brevipes crude extracts. A) Zone of inhibition produced using agar well diffusion method. B) Antibacterial activity screened using only solvents as negative control. C) Zone of inhibition produced using disc diffusion method.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/48/22/178-1656491348-Figure2.jpg",
"caption": "Figure 2. Antimicrobial activity of crude extracts of T. brevipes vs combined crude extracts. A) Zone of inhibition produced by different parts of the plant from different solvents. B) Zone of inhibition produced when different parts from the same solvent are combined.",
"featured": false
}
],
"authors": [
{
"id": 675,
"affiliation": [
{
"affiliation": "Department of Biomedical Laboratory Sciences, INES-Ruhengeri, Musanze, Rwanda"
}
],
"first_name": "Hiberte",
"family_name": "Migabo",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 162
},
{
"id": 676,
"affiliation": [
{
"affiliation": "Department of Molecular Microbiology, Kagawa University Graduate School of Medicine, Japan"
}
],
"first_name": "Emmanuel",
"family_name": "Munyeshyaka",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 162
},
{
"id": 677,
"affiliation": [
{
"affiliation": "Department of Biomedical Laboratory Sciences, INES-Ruhengeri, Musanze, Rwanda"
}
],
"first_name": "Cedrick",
"family_name": "Izere",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 162
},
{
"id": 678,
"affiliation": [
{
"affiliation": "Department of Public Health, Kampala University, Uganda"
}
],
"first_name": "Callixte",
"family_name": "Yadufashije",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 162
},
{
"id": 679,
"affiliation": [
{
"affiliation": "Department of Math, Science and PE, CE, University of Rwanda, Rwanda"
}
],
"first_name": "François N.",
"family_name": "Niyonzima",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 162
},
{
"id": 680,
"affiliation": [
{
"affiliation": "Department of Biomedical Laboratory Sciences, INES-Ruhengeri, Musanze, Rwanda"
}
],
"first_name": "Thierry",
"family_name": "Habyarimana",
"email": "h.thierry@ines.ac.rw",
"author_order": 6,
"ORCID": "http://orcid.org/0000-0001-5488-5578",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Thierry Habyarimana, Department of Biomedical Laboratory Sciences, INESRuhengeri, Musanze, Rwanda, e-mail: h.thierry@ines.ac.rw",
"article": 162
}
]
},
{
"id": 159,
"slug": "178-1660366351-epidemiological-burden-risk-factors-and-recent-therapeutic-advances-in-chronic-obstructive-pulmonary-disease",
"type": "review_article",
"manuscript_id": "178-1660366351",
"published": "2022-10-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/46/178-1660366351.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 109-122.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Loganathan AB, Abdul NR. Epidemiological burden, risk factors, and recent therapeutic advances in chronic obstructive pulmonary disease. J Adv Biotechnol Exp Ther. 2023; 6(1): 109-122.",
"title": "Epidemiological burden, risk factors, and recent therapeutic advances in chronic obstructive pulmonary disease",
"abstract": "<p>Chronic obstructive pulmonary disease (COPD) is a progressive disease that is certainly preventable and treatable and is caused due to the continuous exposure to noxious substances and toxic gases and is characterized by airflow limitation and chronic inflammation in the lungs coupled with persistent symptoms in the respiratory tract leading to obstructive bronchiolitis and parenchymal emphysema. The incidence and progression of COPD is a complicated pathological phenomenon, and the overall severity is due to its exacerbations and comorbidities in individuals. Further, COPD is a major contributor to the global years of life lost and by 2030 it would be the third leading causes of mortality in the world. Also, knowledge on COPD, its associated conditions, and the clinical understanding of the disease date back to the 16th century. The prevalence, morbidity and mortality may vary across the globe based on their exposure to smoking, tobacco, occupational pollutants, indoor pollution, outdoor air pollution, gender, age, and genetic inheritance. Considering the continuous exposure to toxic substances and aging of the general population, the burden and prevalence of COPD are estimated to increase substantially in the coming years. Furthermore, COVID-19 patients with pre-existing COPD conditions suffer from severe disease progression and delay in recovery.</p>",
"DOI": "10.5455/jabet.2023.d110",
"views": 2066,
"downloads": 271,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/08/22/178-1660366351-Figure1.jpg",
"caption": "Figure 1. Burden of COPD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/08/22/178-1660366351-Figure2.jpg",
"caption": "Figure 2. Risk factors of COPD.",
"featured": false
}
],
"authors": [
{
"id": 665,
"affiliation": [
{
"affiliation": "Biopharmaceuticals Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu, India"
}
],
"first_name": "Bakthavatchalam Loganathan",
"family_name": "Ayilya",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 159
},
{
"id": 666,
"affiliation": [
{
"affiliation": "Biopharmaceuticals Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu, India"
}
],
"first_name": "Rasool Abdul",
"family_name": "Nazeer",
"email": "nazeerr@srmist.edu.in",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-0587-3770",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Rasool Abdul Nazeer, PhD; Biopharmaceuticals Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and\r\nTechnology, Kattankulathur Tamilnadu, India, e-mail: nazeerr@srmist.edu.in",
"article": 159
}
]
},
{
"id": 161,
"slug": "178-1660395372-the-potentials-of-pangi-leaf-extract-for-aedes-spp-mosquito-control",
"type": "original_article",
"manuscript_id": "178-1660395372",
"published": "2022-10-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/56/178-1660395372.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 133-139.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Kaitana Y, Kurnia N, et al. The potentials of pangi leaf extract for Aedes spp. mosquito control. J Adv Biotechnol Exp Ther. 2023; 6(1): 133-139.",
"title": "The potentials of pangi leaf extract for Aedes spp. mosquito control",
"abstract": "<p><em>Aedes</em> spp. are the primary vectors of arboviruses such as dengue, Zika, and chikungunya. To date, neither an approved vaccine nor a drug that can definitively prevent or treat these diseases. Vector control continues to be an essential method of disease prevention. Plant-based insecticides are an alternative to chemical insecticides because they are less toxic to non-target insects and degrade more quickly. The Papuan people have used Pangi leaves for generations as a potent natural chemical against head lice and mosquito larvae. This study aimed to evaluate the potential of pangi (<em>Pangium edule</em>) leaf extract as a biolarvicide and mosquito repellent. Fresh pangi leaves were extracted with water by using a blender to pulverize the leaves. One-way ANOVA was used to examine the variance in mortality rates, and the LC<sub>50 </sub>value was calculated for probit analysis. Dead larvae were identified and counted. In addition to testing the effectiveness of the extracts as biolarvicide, the extract was also tested as potential insect repellents. The findings demonstrated that pangi leaf extract can kill mosquito larvae. The concentration of the extract affected larval mortality. Furthermore, the extract demonstrated its effectiveness as an insect repellent. Pangi leaf extract contains alkaloids, flavonoids, tannins, saponins, phenolic compounds, and cyanide compounds, with alkaloids being the most abundant. The study found that there was a direct correlation between the concentration of the insecticide and the number of dead <em>Aedes</em> spp. larvae. The LC<sub>50</sub> probit test revealed that pangi leaf extract belongs to the category of toxic substances. Several compounds, including alkaloids, flavonoids, tannins, saponins, and cyanide, may be responsible for the toxicity of pangi leaf extract. Thus, pangi leaf extract might be established as a means of overcoming numerous health issues caused by mosquito vectors.</p>",
"DOI": "10.5455/jabet.2023.d112",
"views": 1046,
"downloads": 122,
"figures": [],
"authors": [
{
"id": 669,
"affiliation": [
{
"affiliation": "Entomology Study Program, Sam Ratulangi University, Manado 95115, Indonesia"
}
],
"first_name": "Yance",
"family_name": "Kaitana",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 161
},
{
"id": 670,
"affiliation": [
{
"affiliation": "Entomology Study Program, Sam Ratulangi University, Manado 95115, Indonesia"
}
],
"first_name": "Nia",
"family_name": "Kurnia",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 161
},
{
"id": 671,
"affiliation": [
{
"affiliation": "Entomology Study Program, Sam Ratulangi University, Manado 95115, Indonesia"
}
],
"first_name": "Max",
"family_name": "Tulung",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 161
},
{
"id": 672,
"affiliation": [
{
"affiliation": "Department of Parasitology, Faculty of Medicine, Sam Ratulangi University, Manado 95115, Indonesi"
}
],
"first_name": "Josef Sem Berth",
"family_name": "Tuda",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 161
},
{
"id": 673,
"affiliation": [
{
"affiliation": "Entomology Study Program, Sam Ratulangi University, Manado 95115, Indonesia"
}
],
"first_name": "Juliet Merry Eva",
"family_name": "Mamahit",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 161
},
{
"id": 674,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, Indonesia"
}
],
"first_name": "Trina Ekawati",
"family_name": "Tallei",
"email": "trina_tallei@unsrat.ac.id",
"author_order": 6,
"ORCID": "http://orcid.org/0000-0002-7963-7527",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Trina Ekawati Tallei, PhD; Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado-95115, Indonesia, e-mail: trina_tallei@unsrat.ac.id",
"article": 161
}
]
},
{
"id": 160,
"slug": "178-1659609890-assessment-of-antiproliferative-and-toxic-effects-of-a-peptide-from-momordica-dioica-using-in-vitro-and-in-vivo-studies",
"type": "original_article",
"manuscript_id": "178-1659609890",
"published": "2022-10-23",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/39/178-1659609890.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 123-132.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Rupachandra S, Jagadeeshwari S. Assessment of antiproliferative and toxic effects of a peptide from Momordica dioica using in vitro and in vivo studies. J Adv Biotechnol Exp Ther. 2023; 6(1): 123-132.",
"title": "Assessment of antiproliferative and toxic effects of a peptide from Momordica dioica using in vitro and in vivo studies",
"abstract": "<p>Inflammation occurs during a cascade reaction to cause damage to the tissues. Increased oxidant and cytokine expression were observed in the damaged tissues. Inflammatory bowel disease (IBD) may be a chronic and lethal disease of inflammation in gastro enteric tissue characterized by intestinal inflammation. The objective of the study was to investigate the effects of the peptide from <em>Momordica dioica</em> in treating ulcerative colitis. The isolated protein was digested with trypsin enzyme and the hydrolyzed peptide were analyzed using LCMS where the peak obtained at 678.67Da at 17mins and MALDI-TOF analysis which showed similar peaks at 3388.7Da. The results from the MTT assay showed the IC<sub>50</sub> value of the peptide at 100µg/mL. Similarly, the acridine orange staining showed decreased green-fluorescent nuclei cells than red fluorescent acidic vesicular organelles indicating autophagy-dependent cell death. The peptide displayed a cell viability effect on Colo-205 cells at 100 μg/mL when compared to the control. In the acute toxicity test, the mice were orally receiving peptide at a dose of 50-250 mg/kg BW for 14 days. The significant adverse effects were evident at a dose of 250mg/kg BW indicating that the LD<sub>50</sub> value is lesser than 250 mg/kg. Based on the results obtained from this study, the peptide with a molecular mass of 3388.7Da exhibited an increased rate of cytotoxicity and autophagy induction in cancer cells at the concentration of 100 μg/mL. Therefore, the peptide can be further used for the formulation of anticancer drug for treating ulcerative colitis conditions.</p>",
"DOI": "10.5455/jabet.2023.d111",
"views": 905,
"downloads": 173,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/25/178-1659609890-Figure1.jpg",
"caption": "Figure 1. A) HPLC analysis of peptide from the seeds of Momordica dioica showing peaks at a later elution time after 15 min, indicates hydrophobic peptide. B) LC-MS analysis of the peptide from the seeds of Momordica dioica at 17 min depicts the molecular mass of 678.67Da.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/25/178-1659609890-Figure2.jpg",
"caption": "Figure 2. MALDI-TOF analysis of peptide from seeds of Momordica dioica shows the molecular weight of the peptide at 3388.7Da.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/25/178-1659609890-Figure3.jpg",
"caption": "Figure 3. Antiproliferative effect of peptide on the viability of colon cancer cells. Results are represented as mean ± SE for significant differences (P< 0.001) among treatment and cell control (untreated).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/25/178-1659609890-Figure4.jpg",
"caption": "Figure 4. Fluorescence 40X microscopic images of Colo205 cells stained with acridine orange. Control, LPS 1 μg/mL (Positive control), LPS+P25µg/mL, LPS+P50µg/mL, LPS+P75µg/mL, LPS+P100µg/mL, LPS+P125µg/mL. Magnification: 10X, P: Peptide, LPS: Lipopolysaccharide, arrow indicates nuclei; star indicates acidic vesicular organelles.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/25/178-1659609890-Figure5.jpg",
"caption": "Figure 5. Photomicrograph of H&E staining 40X of peptide treated colon tissue (control, peptide-50mg/kg, peptide-100mg/kg, peptide-150mg/kg, peptide-200mg/kg, peptide-250mg/kg) with normal histological appearance.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/25/178-1659609890-Figure6.jpg",
"caption": "Figure 6. Photomicrograph of H&E staining (40X) of peptide treated kidney tissue (control, peptide-50 mg/kg, peptide-100 mg/kg, peptide-150 mg/kg, peptide-200 mg/kg, peptide-250 mg/kg) with conserved tubular and glomerular structure.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/25/178-1659609890-Figure7.jpg",
"caption": "Figure 7. Photomicrograph of H&E staining 40X of peptide treated liver (control, peptide-50 mg/kg, peptide-100 mg/kg, peptide-150 mg/kg, peptide-200 mg/kg, peptide-250 mg/kg) with normal microscopic appearance and black arrow indicates central and portal vein congestion and mild periportal inflammation.",
"featured": false
}
],
"authors": [
{
"id": 667,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, India"
}
],
"first_name": "S.",
"family_name": "Rupachandra",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "S. Rupachandra, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, India, e-mail: rupachas@srmist.edu.in",
"article": 160
},
{
"id": 668,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, India"
}
],
"first_name": "S.",
"family_name": "Jagadeeshwari",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 160
}
]
},
{
"id": 158,
"slug": "178-1660926827-evaluation-of-diagnostic-accuracy-of-ns1-antigen-and-oxidative-stress-for-dengue-virus-infection-in-bangladeshi-population",
"type": "original_article",
"manuscript_id": "178-1660926827",
"published": "2022-09-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/23/178-1660926827.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 94-108.",
"academic_editor": "Md. Masudur Rahman, PhD; Obihiro University of Agriculture & Veterinary Medicine, Japan",
"cite_info": "Rahman T, Kundu SK, et al. Evaluation of diagnostic accuracy of NS1 antigen and oxidative stress for Dengue virus infection in Bangladeshi population. J Adv Biotechnol Exp Ther. 2023; 6(1): 94-108.",
"title": "Evaluation of diagnostic accuracy of NS1 antigen and oxidative stress for Dengue virus infection in Bangladeshi population",
"abstract": "<p>The dramatic rise of global incidence of dengue infection has generated an urgent yet an unmet need for an accurate and validated diagnostic test for DENV infection. A total of 81 serum samples were enlisted to dengue NS1 ICT test and dengue NS1 ELISA test and oxidative stress markers including NO, MDA and SOD activity were measured. Out of 81 samples, 41 were found positive by ELISA (DENV group; n=41) whereas 28 were found positive by ICT test. Out of 81 samples; 40 were found negative (control group; n=40) by ELISA whereas 53 were found negative by ICT test. There were 28 samples which were found positive by both ICT and ELISA test. There were 40 samples which were found negative by both ICT and ELISA. The sensitivity and specificity of ICT test were 68.29% and 100% when compared with ELISA. The positive cases of dengue were confirmed by platelet count having significantly higher level of platelet counts (P<0.0001) in dengue patients compared to that of control. Dengue patients had significantly higher levels of NO (P=0.0007), MDA (P<0.0001) and SOD activity (P<0.0001) compared to those of control. Among dengue patients, a positive correlation was found between MDA level and hematocrit percentage (R2 = 0.8107; P<0.001) and between MDA level and NO level (R2 = 0.4954; P<0.001). The study has unveiled that though the rapid ICT test is cost-effective and with satisfactory specificity, the sensitivity is not up to the mark and possibility of misdiagnosis by this rapid test kit is significant.</p>",
"DOI": "10.5455/jabet.2023.d109",
"views": 1765,
"downloads": 155,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/25/25/178-1660926827-Figure1.jpg",
"caption": "Figure 1. Comparison of the platelet count between the control and the DENV patient group. The control subject had a mean platelet count of 282800 cells/μL while the patient group had a mean platelet of 173500 cells/μL. The patient group had a significantly lower amount of platelet (P< 0.0001).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/25/25/178-1660926827-Figure2.jpg",
"caption": "Figure 2. Venn diagram showing positive, negative and total cases in DENV NS1 rapid ICT test assay and DENV NS1 ELISA assay.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/25/25/178-1660926827-Figure3.jpg",
"caption": "Figure 3. Comparison of serum nitric oxide level (A), MDA level (B), and serum SOD activity (C) in two group of study subjects with their standard deviations within each group. The DENV patient group had significantly higher nitric oxide level (p=0.0007) (A) and higher MDA level (p<0.0001) (B), and higher level of SOD activity (p<0.0001) in serum than the control group. Correlation between malondialdehyde and hematocrit percentage (D), nitric oxide and MDA levels (E), and nitric oxide and SOD activity (F) among dengue patients with a coefficient of determination of 0.8107 (P<0.001) (D), 0.4958 (P< 0.001) (E) and 0.2982 (P=0.0002) (F), respectively.",
"featured": false
}
],
"authors": [
{
"id": 661,
"affiliation": [
{
"affiliation": "Infection and Immunology laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Tania",
"family_name": "Rahman",
"email": "tania.rahman@du.ac.bd",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-6398-3214",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Tania Rahman, PhD; Associate Professor, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh, e-mail: tania.rahman@du.ac.bd",
"article": 158
},
{
"id": 662,
"affiliation": [
{
"affiliation": "Department of Environmental Science and Management, North South University, Dhaka, Bangladesh"
}
],
"first_name": "Sourav Kumar",
"family_name": "Kundu",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 158
},
{
"id": 663,
"affiliation": [
{
"affiliation": "Department of Environmental Science and Management, North South University, Dhaka, Bangladesh"
}
],
"first_name": "Md Ferdous",
"family_name": "Seraj",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 158
},
{
"id": 664,
"affiliation": [
{
"affiliation": "Infection and Immunology laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Md",
"family_name": "Mohasin",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 158
}
]
},
{
"id": 1,
"slug": "178-1655275227-biofilm-formation-by-the-interaction-of-fungi-candida-tropicalis-with-various-bacteria",
"type": "original_article",
"manuscript_id": "178-1655275227",
"published": "2022-09-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/14/178-1655275227.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 84-93.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka 1230, Bangladesh",
"cite_info": "Asih DW, Widodo ADW, et al. Biofilm formation by the interaction of fungi (Candida tropicalis) with various bacteria. J Adv Biotechnol Exp Ther. 2023; 6(1): 84-93.",
"title": "Biofilm formation by the interaction of fungi (<span>Candida tropicalis</span>) with various bacteria",
"abstract": "<p>Biofilms are composed of more than one species and are often called polymicrobial biofilms, so research is needed on the formation of polymicrobial biofilms, especially between fungi and bacteria. This study aimed to analyze the total biomass and metabolic activity of biofilms formed from the interaction of fungi (<em>Candida tropicalis</em>) with various bacteria, including <em>Staphylococcus aureus</em>, methicillin-resistant <em>Staphylococcus aureus</em>, <em>Pseudomonas aeruginosa</em>, and <em>Acinetobacter baumannii</em>. Bacterial and fungal cultures were suspended in tryptic soy broth (TSB) medium, and biofilm was cultivated in two 96-well microplates for 48 hours at 37°C. The crystal violet assay was used to detect the total biomass biofilm, and the tetrazolium salt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, or MTT) assay was used to obtain the metabolic activity of biofilm with the optical density (OD) value using an ELISA reader. The results of this study obtained significant data from both parameters. The highest average value was found in the <em>C. tropicalis</em> treatment group (2,412 ± 0.825 on the biomass test results; 1,525 ± 0.473 on the metabolic activity test results), while the lowest was seen in the <em>S. aureus</em> treatment group (0.512 ± 0.224 in the biomass test results; 0.978 ± 0.349 in the metabolic activity test results). In conclusion, it was confirmed that biofilm biomass could be generated strongly in each treatment in the total biomass parameter, while metabolic activity data suggested that each treatment could carry out cell proliferation.</p>\r\n<p> </p>",
"DOI": "10.5455/jabet.2023.d108",
"views": 2195,
"downloads": 333,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/46/25/178-1655275227-Figure1.jpg",
"caption": "Figure 1. Total biomass by CV staining for single-species and mixed-species biofilms where OD isolate > 4xODc (0.072) = high biofilm forming. Data were obtained based on *P-value < 0.05 (compared with control) which was considered statistically significant.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/46/25/178-1655275227-Figure2.jpg",
"caption": "Figure 2. Metabolic activity by MTT assay for single-species and mixed-species biofilms where OD < 0.079=low cell proliferation, 0.079 < OD < 1.25= Normal cell proliferation, OD > 1.25= Increased cell proliferation. Data was obtained based on *P-value < 0.05 (compared with control) which was stated to be statistically significant.",
"featured": false
}
],
"authors": [
{
"id": 1,
"affiliation": [
{
"affiliation": "Department of Basic Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Dwi Wahyuning",
"family_name": "Asih",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-3989-9266",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 1
},
{
"id": 2,
"affiliation": [
{
"affiliation": "Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia"
}
],
"first_name": "Agung Dwi Wahyu",
"family_name": "Widodo",
"email": "agungimunologi@gmail.com",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Agung Dwi Wahyu Widodo, PhD; Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia, e-mail: agungimunologi@gmail.com",
"article": 1
},
{
"id": 3,
"affiliation": [
{
"affiliation": "Department of Medical Microbiology, Clinical Microbiology Specialist Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Rebekah Juniati",
"family_name": "Setiabudi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 1
},
{
"id": 576,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Sulistiawati",
"family_name": "M",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 1
},
{
"id": 577,
"affiliation": [
{
"affiliation": "Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Wiwiek",
"family_name": "Tyasningsih",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 1
},
{
"id": 578,
"affiliation": [
{
"affiliation": "Department of Medical Microbiology, Clinical Microbiology Specialist Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia"
}
],
"first_name": "Manik Retno",
"family_name": "Wahyunitisari",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 1
}
]
},
{
"id": 157,
"slug": "178-1657172458-anti-inflammatory-anti-oxidative-and-anti-microbial-activities-of-the-phytochemicals-isolated-from-various-parts-of-broccoli-wastes",
"type": "original_article",
"manuscript_id": "178-1657172458",
"published": "2022-09-04",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/03/178-1657172458.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 67-83.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Ramesh A, Nandi A, et al. Anti-inflammatory, anti-oxidative, and anti-microbial activities of the phytochemicals isolated from various parts of broccoli wastes. J Adv Biotechnol Exp Ther. 2023; 6(1): 67-83.",
"title": "Anti-inflammatory, anti-oxidative, and anti-microbial activities of the phytochemicals isolated from various parts of broccoli wastes",
"abstract": "<p>Broccoli is an excellent source of vitamins, minerals, phytochemicals, and nutraceuticals. Phytochemicals, also known as phytonutrients, are chemical compounds that are present in fruits, vegetables, and other plants and can be broadly classified into carbohydrates, terpenoids, phenolics, lipids, alkaloids, and other nitrogen-containing compounds. Waste generation is a global problem and vegetable wastes that contain the same or higher amount of phytonutrients as the vegetable itself are discarded on daily basis leading to additional waste biomass. The unused portion of broccoli is considered to be waste which includes the stalk and the leaves, rotten and scraped portions of the vegetable. Only the fragile stems closest to the florets are eaten, while the lignified bottom stem of the vegetable is discarded. Approximately 30% of vegetable loss is generated at retail and consumer levels including post- harvest and processing. These wastes possess various nutrients and multiple bioactive compounds such as phytochemicals (phenolics, glycosylates, carotenoids, and flavonoids). Broccoli waste has an enormous amount of these nutrients and nutraceuticals which have a wider range of applications in food supplements, pharmaceuticals, and cosmetic industries. The phytochemicals from the leaf and stalk were extracted using the maceration process, and the stalk extract had stronger antioxidant and anti-inflammatory activity at a concentration of 500 g/ml. It also showed good anti-microbial activity at 300 μg/ml proving it to be a potential source of important bioactive compounds and implying the presence of anti-aging and anti-acne activity.</p>",
"DOI": "10.5455/jabet.2023.d107",
"views": 2104,
"downloads": 233,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1657172458-Figure1.jpg",
"caption": "Figure 1. Comparison of total carotenoids, chlorophyll b, and chlorophyll a content (mg/g) in the (A) leaf sample and (B) stalk sample.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1657172458-Figure2.jpg",
"caption": "Figure 2. GCMS results for leaf sample.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1657172458-Figure3.jpg",
"caption": "Figure 3. GCMS results for stalk sample.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1657172458-Figure4.jpg",
"caption": "Figure 4. Comparison of anti-inflammatory activity of broccoli leaf and stalk extract against standard drug using (A) inhibition of albumin denaturation activity, (B) inhibition of proteinase activity, and (C) membrane stabilization activity. P <0.05 vs control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1657172458-Figure5.jpg",
"caption": "Figure 5. Comparison of antioxidative properties of broccoli leaf and stalk extract against standard drug using (A) DPPH assay and (B) FRAP assay. P <0.05 vs control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1657172458-Figure6.jpg",
"caption": "Figure 6. Antibacterial activity of test samples against S. aureus: a: 0 mg/mL; b: 0.1 mg/mL; c: 0.2 mg/mL; d: 0.3 mg/mL; e: 0.4 mg/mL; g: Azithromycin (0.03 mg/mL).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1657172458-Figure7.jpg",
"caption": "Figure 7. A) Activity of test samples against S. aureus at Minimum Inhibitory Concentrations; Arrow indicating the No visible growth- Column 1 -10 (2 mg/mL to 0.004 m g/mL); Column 11: Extract free Control; Column 12: Sterility control; Rows A & B: Stalk Extract; Rows F & G: Leaf Extract. B) minimum bactericidal concentrations activity of test sample Stalk extract against S. aureus.",
"featured": false
}
],
"authors": [
{
"id": 656,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Aishwarya",
"family_name": "Ramesh",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 157
},
{
"id": 657,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Asmita",
"family_name": "Nandi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 157
},
{
"id": 658,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Srirangasayee",
"family_name": "Devanathan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 157
},
{
"id": 659,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Lavanya",
"family_name": "Jayaraman",
"email": "lavanyaj@srmist.edu.in",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Lavanya Jayaraman, PhD; Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India, e-mail: lavanyaj@srmist.edu.in",
"article": 157
},
{
"id": 660,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Subhashini",
"family_name": "Sivaji",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 157
}
]
},
{
"id": 156,
"slug": "178-1656165066-detection-and-biological-control-measures-of-anthracnose-causing-fungus-isolated-from-citrus-limon-l",
"type": "original_article",
"manuscript_id": "178-1656165066",
"published": "2022-09-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/46/178-1656165066.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 58-66.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Hasan MF, Ferdous J, et al. Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.).J Adv Biotechnol Exp Ther. 2023; 6(1): 58-66.",
"title": "Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.)",
"abstract": "<p>Anthracnose is one of the most devastating fungi causing twig dieback and postharvest fruit decay. Present study was aimed to identification of anthracnose fungus. Infected leaves of <em>Citrus limon</em> were collected and cultured on potato dextrose agar (PDA) media for pathogenic fungus isolation. The isolated fungal pure culture was characterized by physiological and morphological characterization methods. Biological control measures of the fungus were evaluated by disc diffusion methods. The highest growth and development of isolated fungus was detected in PDA media at pH 7 in fructose as the best carbon source and 0.05gm NaCl concentrations at 37°C. Pathogenicity potency of isolate was performed on lemon, orange and malta, and showed typical anthracnose symptoms after incubation at 25°C for 5 days. For antifungal activity, 200µgm/disc methanolic extract of <em>Psidium guajava </em>showed 14.33±0.66 mm inhibition zone against the isolated fungus. From the present investigations, identified anthracnose causing fungus and it’s controlling techniques may help for further research for the isolation of drugs related compound for controlling this disease.</p>",
"DOI": "10.5455/jabet.2023.d106",
"views": 1363,
"downloads": 128,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1656165066-Figure1.jpg",
"caption": "Figure 1. Schematic represents the isolated fungus: (a) inside the culture plate 7 days after inoculation; and (b) pycnidia.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1656165066-Figure2.jpg",
"caption": "Figure 2. Effect of different growth factors on fungus culture, growth, and development; (A) media, (B) Tm, (C) pH levels, (D) carbohydrates, (E) NaCl and (F) lactic acid. The mycelium weight was measured after 7 days of inoculation.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1656165066-Figure3.jpg",
"caption": "Figure 3. The isolated fungus showed the pathogenicity against the fruits: (A) Controls; (B) Lemon; (C) Orange, and (D) Malta.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1656165066-Figure4.jpg",
"caption": "Figure 4. (A) Antifungal activities of plant extracts (a) Coccinia grandis, (b) Azadirachta indica, (c) Hibiscus rosa-sinensis, (d) Psidium guajava, (e) Cassia alata and (f) Spondias mombin. (B) Different plants extract showing zone of inhibitions (in mm) against the isolated fungus.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/01/28/178-1656165066-Figure5.jpg",
"caption": "Figure 5. Antagonistic test of soil borne bacteria against the isolated fungus: (A) Rhizobium leguminosarum; and (B) Rhizobium phaseoli.",
"featured": false
}
],
"authors": [
{
"id": 650,
"affiliation": [
{
"affiliation": "Department of Microbiology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md Faruk",
"family_name": "Hasan",
"email": "faruk_geb@ru.ac.bd",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0003-2746-5994",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 156
},
{
"id": 651,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Jannatul",
"family_name": "Ferdous",
"email": "jannatulfkhushi@gmail.com",
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 156
},
{
"id": 652,
"affiliation": [
{
"affiliation": "Department of Microbiology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Amit Kumar",
"family_name": "Dutta",
"email": "amitdutta_81@yahoo.com",
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 156
},
{
"id": 653,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Fahmida Begum",
"family_name": "Mina",
"email": "fahmidamina10@gmail.com",
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 156
},
{
"id": 654,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Sumon",
"family_name": "Karmakar",
"email": "sumonkr6@gmail.com",
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 156
},
{
"id": 655,
"affiliation": [
{
"affiliation": "Department of Microbiology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Biswanath",
"family_name": "Sikdar",
"email": "bisw69@ru.ac.bd",
"author_order": 6,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Biswanath Sikdar, PhD; Department of Microbiology, University of Rajshahi, Rajshahi 6205, Bangladesh, e-mail: bisw69@ru.ac.bd",
"article": 156
}
]
},
{
"id": 154,
"slug": "178-1648149750-developing-a-multiepitope-vaccine-against-dengue-virus-in-bangladesh-using-immunoinformatics-approach",
"type": "original_article",
"manuscript_id": "178-1648149750",
"published": "2022-08-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/11/178-1648149750.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 44-57.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Akash SR, Hossain MI, et al. Developing a multiepitope vaccine against dengue virus in Bangladesh using immunoinformatics approach. J Adv Biotechnol Exp Ther. 2023; 6(1): 44-57.",
"title": "Developing a multiepitope vaccine against dengue virus in Bangladesh using immunoinformatics approach",
"abstract": "<p>Dengue fever is a devastating mosquito-borne illness that has claimed the lives of countless people. The virus responsible for this disease is a member of the Flaviviridae family, which produces positive-stranded RNA. Dengue fever is an exquisite viral fever caused by the bite of an Aedes mosquito carrying one of four serotypes of dengue virus. This virus is transmitted via a vertical route utilizing a comprehensively unique system. Unfortunately, no effective vaccine has yet been developed to eradicate this disease. This study employed computational methods to design and propose a multi-epitope vaccine against dengue virus in Asia. This study utilized various immunoinformatics databases to predict potent epitopes on the envelope protein of the dengue virus using in silico methods. We identified a total of 14 epitopes from the target envelope protein by assessing their ability to induce both innate and acquired immunity through T- and B-lymphocyte-mediated responses. Because dengue virus is an RNA virus, epitope conservation was considered, and all selected epitopes were 100 percent conserved. The antigenicity of the final component of the multi-epitope vaccine was 0.7055. To improve the stability of the vaccine protein, disulfide engineering was performed in a region with high mobility. Additionally, codon adaptation and in silico cloning ensure that the proposed subunit vaccine will be expressed at a higher level in <em>E. coli</em>. In order to evaluate the binding free energy and stability of the combination, the vaccine protein and TLR-4 receptor were subjected to a molecular docking simulation. In order to establish active immunity against the dengue virus, the proposed in silico vaccine must be tested for safety and immunogenicity.</p>",
"DOI": "10.5455/jabet.2023.d105",
"views": 1356,
"downloads": 222,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure1.jpg",
"caption": "Figure 1. Schematic depiction of our workflow procedure.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure2.jpg",
"caption": "Figure 2. Graphical outline of the expressed multi-epitope vaccine assembles where it linked with adjuvant, CTL epitopes, HTL epitopes and LBL epitopes (Left to right). Here the adjuvant and CTL were linked by EAAAK linker (pink), CTL epitopes were linked by AAK linker (Blue), HTL epitopes were linked by GPPG (Yellow) and finally LBL epitopes were linked by KK linker (Green).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure3.jpg",
"caption": "Figure 3. Solubility value by protein sol server.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure4.jpg",
"caption": "Figure 4. A) Windowed charge score per amino acid. B) Windowed fold propensity score per amino acid. C) Secondary structure prediction report.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure5.jpg",
"caption": "Figure 5. A) Tertiary structure of designated vaccine. Different secondary structure of amino acids is identified as different color, like a-helix as red color, b-strand as yellow color and random coil as green color. B) Refined Tertiary structure of designated vaccine.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure6.jpg",
"caption": "Figure 6. Validation of the tertiary structure of the vaccine.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure7.jpg",
"caption": "Figure 7. Constructed vaccine after molecular docking.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure8.jpg",
"caption": "Figure 8. Molecular dynamics simulation of the vaccine. Here, different MD simulation plots show (A) Molecular deformability on molecular dynamic simulation; (B) B-factor/mobility on molecular dynamic simulation; (C) Eigenvalues on molecular dynamic simulation; (D) Variance on molecular dynamic simulation; (E) Covariance map on molecular dynamic simulation; (F) Elastic network on molecular dynamic simulation.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure9.jpg",
"caption": "Figure 9. Immune response stirred up by the designed vaccine where the graph shows (A) Antigen and immunoglobulins. Antibodies are divided per isotype; (B) B lymphocytes: total measure using selected epitopes; (C) Plasma B lymphocytes measure using the isotype (IgM, IgG1 and IgG2); (D) CD4 T-helper lymphocytes count. The plot shows total and memory counts; (E) CD4 T-regulatory lymphocytes count. Both total, memory and per entity-state counts are plotted here; (F) CD8 T-cytotoxic lymphocytes count. Total and memory shown; and (G) Cytokines Concentration of cytokines and interleukins. D in the inset plot is danger signal.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/28/178-1648149750-Figure10.jpg",
"caption": "Figure 10. Constructed vaccine after cloning. The red section indicates the codon-optimized multi-epitope vaccine that has been introduced into the pET-28a (+) expression vector.",
"featured": false
}
],
"authors": [
{
"id": 642,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Bangladesh University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Sajidur Rahman",
"family_name": "Akash",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 154
},
{
"id": 643,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj8100, Bangladesh"
}
],
"first_name": "Md Imran",
"family_name": "Hossain",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 154
},
{
"id": 644,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj8100, Bangladesh"
}
],
"first_name": "Md Sarafat",
"family_name": "Ali",
"email": "sarafatbiotech@bsmrstu.edu.bd",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Sarafat Ali, PhD; Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University,\r\nGopalganj-8100, Bangladesh, e-mail: sarafatbiotech@bsmrstu.edu.bd",
"article": 154
}
]
},
{
"id": 148,
"slug": "178-1653070433-assessment-and-comparison-of-cardiovascular-disease-risk-factors-and-biochemical-parameters-among-men-and-women-a-cross-sectional-study",
"type": "original_article",
"manuscript_id": "178-1653070433",
"published": "2022-08-12",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/50/178-1653070433.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 25-34.",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Islam S, Nobel FA, et al. Assessment and comparison of cardiovascular disease risk factors and biochemical parameters among men and women: A cross-sectional study. J Adv Biotechnol Exp Ther. 2023; 6(1): 25-34.",
"title": "Assessment and comparison of cardiovascular disease risk factors and biochemical parameters among men and women: A cross-sectional study",
"abstract": "<p>Cardiovascular disease (CVD) is one of the most common causes of death among men and women worldwide. It is predicted that by 2030 around 23.3 million people will die as a consequence of CVD. There are numerous risk factors for CVD. The goal of this study is to examine the cardiovascular clinical and biochemical parameters of hospitalized CVD patients, as well as to assess the most common risk factors. Seventy-two known healthy individuals were randomly allocated to the control group (male 47 and female 25). We collected data through a questionnaire from 154 CVD patients as a study group (male 108 and female 46). Cardiovascular status was assessed using clinical parameters like hypertension, chest pain, shortness of breathing, pain in the arm, and biochemical parameters like lipid profile, RBS, creatinine, and an electrolyte panel. Inter- and intra-group comparison was performed. A total of 154 hospitalized CVD patients were analyzed (male 108 female 46) The most significant age group for males was observed as 56-65 years (mean 60) and for females was 38-48 (mean 43) years. A highly statistically significant increase was observed in total cholesterol and LDL in males than in females, but a decrease in TG than in females. Changes were also observed in other cardiovascular biochemical and clinical parameters. There was a significant difference in smoking status, physical activity, lipid panel, and other biochemical parameters among males and females. It is concluded that studies investigating the observed sex differences in traditional risk factor effects would not only help us better understand the etiology of CVD but also help us understand how to prevent it.</p>",
"DOI": "10.5455/jabet.2023.d103",
"views": 1280,
"downloads": 210,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/29/178-1653070433-Figure1.jpg",
"caption": "Figure 1. Represents the frequency of CVD patients based on age. The age group 56-65 accounts for the majority of CVD patients, accounting for 27% of all patients. The second major age group associated with CVD is 46-55.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/29/178-1653070433-Figure2.jpg",
"caption": "Figure 2. The percentage of patients who are connected to smoking is depicted in the pie chart. Nearly half of all patients (47%) were current smokers.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/29/178-1653070433-Figure3.jpg",
"caption": "Figure 3. Comparison of physical activity between male and female patients. The number of males who frequently perform physical exercise is greater than females, besides more females never did exercise than males.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/29/178-1653070433-Figure4.jpg",
"caption": "Figure 4. Comparison of medical history between male and female patients. More males experienced chest pain and fatigue & weakness than females, shortness of breathing was higher in females.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/29/178-1653070433-Figure5.jpg",
"caption": "Figure 5. Comparison of Lipid panel in control vs diseased male and diseased female. Cholesterol and HDL were higher in females than in males, but TG and LDL were lower.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/29/178-1653070433-Figure6.jpg",
"caption": "Figure 6. Comparison of electrolytes among control group, diseased male, and female. Na, K, and Cl levels were higher in diseased males and females.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/22/29/178-1653070433-Figure7.jpg",
"caption": "Figure 7. Comparison of RBS and creatinine level between control and diseased group. Creatinine level was higher in females than males, but RBS level was higher in males.",
"featured": false
}
],
"authors": [
{
"id": 624,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Saiful",
"family_name": "Islam",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-8490-8619",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 148
},
{
"id": 625,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
},
{
"affiliation": "Infectious Diseases Division, International Centre for Diarrheal Disease Research, Bangladesh, Dhaka-1212"
}
],
"first_name": "Fahim Alam",
"family_name": "Nobel",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-7975-4555",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 148
},
{
"id": 626,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Saima",
"family_name": "Sabrina",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 148
},
{
"id": 627,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Ashekul",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0002-4808-1685",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 148
},
{
"id": 628,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Mohammod Johirul",
"family_name": "Islam",
"email": "johir7479@gmail.com",
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-4808-1685",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammod Johirul Islam, PhD; Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Bangladesh, \r\ne-mail: johir7479@gmail.com",
"article": 148
}
]
},
{
"id": 139,
"slug": "178-1654751274-influence-of-fruits-and-vegetables-waste-pre-treatment-on-black-soldier-fly-larval-growth",
"type": "original_article",
"manuscript_id": "178-1654751274",
"published": "2022-08-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/23/178-1654751274.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 01-08.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Humpy B, Kumar SS, et al. Influence of fruits and vegetables waste pre-treatment on black soldier fly larval growth. J Adv Biotechnol Exp Ther. 2023; 6(1): 01-08.",
"title": "Influence of fruits and vegetables waste pre-treatment on black soldier fly larval growth",
"abstract": "<p><em>Hermetia illuciens</em>, commonly known as black soldier fly (BSF) can convert organic biomass into manure and insect biomass into protein and fat. Currently, in the waste management industry, BSF larvae are predominantly used to convert valorized organic substrates into fertilizer and to produce biogas. They are also alternatively used as viable protein substitutes for animal feed as well as human consumer products. The treatment of organic wastes before BSFL consumption indicated a positive stimulation in the biomass conversion rate, larval growth rate and overall larval performance parameters. The current study was designed to analyze the effect of the bio-stimulation-based pre-treatment strategy of the feed on performance parameters, such as larval weight, larval survival rate, substrate reduction by pre-treatment of biowaste for better growth, efficient performance, and good biomass composition in BSFL. In our study, we noticed significant up- and downregulation of several larval parameters by assessing substrate composition, larval growth parameters and substrate reduction rate. The bio-stimulated waste showed larval weight gain when compared to larvae grown in control feed. The heat pretreated waste was not suitable for larval growth as the parameters assessed were observed to be declined. Further investigations are needed to comprehend how BSFL reared on pretreated substrates enhances the nutritional composition of larvae.</p>",
"DOI": "10.5455/jabet.2023.d100",
"views": 1432,
"downloads": 175,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/29/178-1654751274-Figure1.jpg",
"caption": "Figure 1. Mean survival rate of BSFL in control and treatment groups after 24, 48, and 72h of exposure along with heat pretreated samples. Statistical significance and the results are represented as mean ± SEM. The statistical significance was set at p< 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/29/178-1654751274-Figure2.jpg",
"caption": "Figure 2. Mean larval weight of BSFL observed in control and treatment groups after 24, 48, and 72h of exposure along with heat pretreated samples. Statistical significance and the results are represented as mean ± SEM. The statistical significance was set at p< 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/29/178-1654751274-Figure3.jpg",
"caption": "Figure 3. Substrate reduction of waste analyzed in control and treatment groups after 24h, 48h, 72h of exposure along with heat pretreated samples. Statistical significance and the results are represented as mean ± SEM. The statistical significance was set at p< 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/29/178-1654751274-Figure4.jpg",
"caption": "Figure 4. Schematic representation of BSFL grown on heat Pre-treated waste and bioactivated waste samples.",
"featured": false
}
],
"authors": [
{
"id": 579,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Bulla",
"family_name": "Humpy",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 139
},
{
"id": 580,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Swetha Senthil",
"family_name": "Kumar",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-5816-6674",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 139
},
{
"id": 581,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Sahabudeen Sheik",
"family_name": "Mohideen",
"email": "sahabuds@srmist.edu.in",
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-9921-8039",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sahabudeen Sheik Mohideen, PhD; Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603203, Chengalpattu, Tamil Nadu, India, e-mail: sahabuds@srmist.edu.in",
"article": 139
}
]
},
{
"id": 140,
"slug": "178-1653256308-histological-and-physiological-determinants-of-hypothyroidism-in-patients-and-its-relationship-with-lipid-profile",
"type": "original_article",
"manuscript_id": "178-1653256308",
"published": "2022-08-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/41/178-1653256308.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 09-16.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Hashim AM, Harbi SJA, et al. Histological and physiological determinants of hypothyroidism in patients and its relationship with lipid profile. J Adv Biotechnol Exp Ther. 2023; 6(1): 09-16.",
"title": "Histological and physiological determinants of hypothyroidism in patients and its relationship with lipid profile",
"abstract": "<p>Hypothyroidism is a disorder in which the thyroid gland fails to produce enough thyroid hormone to maintain normal physiological function. Low thyroid hormone levels make it difficult for cells in the body to get enough thyroid hormone, resulting in a slowed metabolism. There are various reasons for the prevalence of autoimmune disease and radiation therapy. Excess or deficiency of iodine causes thyroiditis. Because lipids are hydrophobic, they cannot transport fats like cholesterol, phospholipids, and triglycerides through the bloodstream. Instead, they bind to proteins to produce lipoproteins, which carry fats such as cholesterol, triglycerides, and phospholipids throughout the body. This study evaluated the levels of triiodothyronine (T3), thyroxin (T4), and thyroid-stimulating hormone (TSH) in 25 patients and 25 healthy individuals using the EIA technique. The study found that hypothyroidism patients have higher TSH levels than healthy controls, while their T3 and T4 levels are lower than healthy controls. When hypothyroidism patients were compared to healthy controls, there was a significant increase in the mean levels of all lipids except for high-density lipoprotein. We concluded that the TSH levels in hypothyroidism patients were significantly higher than in the control group. In addition, these patients have an abnormal lipid profile, with significantly higher levels of TC, TG, LDL, and VLDL.</p>",
"DOI": "10.5455/jabet.2023.d101",
"views": 1650,
"downloads": 166,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/07/29/178-1653256308-Figure1.jpg",
"caption": "Figure 1. Distribution of thyroid hormone levels in study groups. * Significant at p ≤ 0.01.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/07/29/178-1653256308-Figure2.jpg",
"caption": "Figure 2. Distribution of lipid parameter levels in the study group. * Significant at p ≤ 0.01.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/07/29/178-1653256308-Figure3.jpg",
"caption": "Figure 3. The thyroid gland tissue section showed the immunohistochemistry staining protein overexpression using biotinylated T3 protein antibody; stained by dab-chromogen (blue) and counterstained by nuclear fast red stain (red). a-normal tissue, b-t3 hypothyroidism dysfunction.",
"featured": false
}
],
"authors": [
{
"id": 582,
"affiliation": [
{
"affiliation": "DNA Research Center, University of Babylon, Hillah, Babylon state, Iraq"
}
],
"first_name": "Adil Mohammed",
"family_name": "Hashim",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 140
},
{
"id": 583,
"affiliation": [
{
"affiliation": "Department of Human Anatomy, College of Medicine, University of Babylon, Hillah, Babylon state, 51001, Iraq"
}
],
"first_name": "Sawsam Jaseim Al",
"family_name": "Harbi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 140
},
{
"id": 584,
"affiliation": [
{
"affiliation": "Department of Human Anatomy, College of Medicine, University of Babylon, Hillah, Babylon state, 51001, Iraq"
}
],
"first_name": "Mariam Mohammed",
"family_name": "Burhan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 140
},
{
"id": 585,
"affiliation": [
{
"affiliation": "DNA Research Center, University of Babylon, Hillah, Babylon state, Iraq"
}
],
"first_name": "Yasir Haider Al",
"family_name": "Mawlah",
"email": "yasser.almawla@uobabylon.edu.iq",
"author_order": 4,
"ORCID": "http://orcid.org/0000-0002-2453-9567",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Yasir Haider Al-Mawlah, PhD; DNA Research Center, University of Babylon, Hillah, Babylon state, Iraq, e-mail: yasser.almawla@uobabylon.edu.iq",
"article": 140
},
{
"id": 586,
"affiliation": [
{
"affiliation": "Department of Human Anatomy, College of Medicine, University of Babylon, Hillah, Babylon state, 51001, Iraq"
}
],
"first_name": "Ameer Mezhir",
"family_name": "Hadi",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 140
}
]
},
{
"id": 147,
"slug": "178-1655358317-oxidant-and-antioxidant-status-of-erythrocytes-and-plasma-samples-in-polycystic-ovary-syndrome-patients",
"type": "original_article",
"manuscript_id": "178-1655358317",
"published": "2022-08-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/27/178-1655358317.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 17-24.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Nawar AS, Obaid ZH, et al. Oxidant and antioxidant status of erythrocytes and plasma samples in polycystic ovary syndrome patients. J Adv Biotechnol Exp Ther. 2023; 6(1): 17-24.",
"title": "Oxidant and antioxidant status of erythrocytes and plasma samples in polycystic ovary syndrome patients",
"abstract": "<p>Polycystic ovary syndrome (PCOS) is one of the most common types of endocrine syndromes in women, where the disease is described as chronic anovulation and hyperandrogenism, which was characterized by the formation of small cysts (fluid-filled sacs) in the ovaries. This study was carried out to introduce knowledge when plasma and erythrocytes portions were chosen to compare the antioxidant and oxidant status of PCOS patients treated or without treatment with metformin. Samples included 75 women diagnosed with PCOS according to Rotterdam criteria. The control group included healthy volunteers with regular menstrual cycles and with no symptoms of hyperandrogenism. Total antioxidants capacity (TAC), total oxidant status (TOS), and Cu\\Zn SOD enzyme activity were examined in both plasma and erythrocytes. The activity of the Cu\\Zn SOD enzyme was determined based on the ability of the enzyme to inhibit the autoxidation of pyrogallol. Body mass index (BMI), hormonal tests (LH and FSH) showed significant differences in PCOS patients as compared to control. In both plasma and erythrocyte, TAC levels were significantly decreased in PCOS patients. Though the activity of superoxide dismutase was significantly higher in plasma and erythrocytes in patients. On the other hand, PCOS patients showed significantly higher levels of TOS in plasma, while erythrocytes did not show any significant differences. In addition, metformin treatment significantly decreased levels of TAC, TOS and SOD1 activity in PCOS patients. Considering all, it is concluded that both TOS and SOD1 activity introduce the same pattern in blood samples, TAC displays a different line of responses which can be due to the crucial role of SOD1 as an antioxidant factor.</p>",
"DOI": "10.5455/jabet.2023.d102",
"views": 1102,
"downloads": 172,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/29/178-1655358317-Figure1.jpg",
"caption": "Figure 1. The total antioxidants capacity (TAC) in both PCOS patients and control group according to the plasma and erythrocytes. **** Significant difference (p ≤0.01).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/29/178-1655358317-Figure2.jpg",
"caption": "Figure 2. The total oxidant status (TOS) in both PCOS patients and control group according to the plasma and erythrocytes. **** Significant difference (p≤0.01).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/29/178-1655358317-Figure3.jpg",
"caption": "Figure 3. The Cu\\Zn Superoxide dismutase enzyme activity in both PCOS patients and control group according to the plasma and erythrocytes. **** Significant difference (p ≤0.01).",
"featured": false
}
],
"authors": [
{
"id": 621,
"affiliation": [
{
"affiliation": "College of Science, University of Babylon, Hilla, Babylon state, 51001, Iraq"
}
],
"first_name": "Aghras S.",
"family_name": "Nawar",
"email": "aghras.s@biotech.uoqasim.edu.iq",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Aghras S. Nawar, College of Science, University of Babylon, Hilla, Babylon state, Iraq, e-mail: aghras.s@biotech.uoqasim.edu.iq",
"article": 147
},
{
"id": 622,
"affiliation": [
{
"affiliation": "College of Science, University of Babylon, Hilla, Babylon state, 51001, Iraq"
}
],
"first_name": "Zeena H.",
"family_name": "Obaid",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 147
},
{
"id": 623,
"affiliation": [
{
"affiliation": "College of Molecular Biology and Biotechnology, University of Sheffield, Sheffield city, United Kingdom"
}
],
"first_name": "Qaiser I.",
"family_name": "Sheikh",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 147
}
]
},
{
"id": 150,
"slug": "178-1654636722-investigation-of-the-relationship-between-matrix-metalloproteinase-9-and-tissue-inhibitor-of-metalloproteinase-1-with-sars-cov-2-infections",
"type": "original_article",
"manuscript_id": "178-1654636722",
"published": "2022-08-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/32/178-1654636722.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2023; 6(1): 35-43.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Ismael MK, Rasuol LM, et al. Investigation of the relationship between matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 with SARS CoV-2 infections. J Adv Biotechnol Exp Ther. 2023; 6(1): 35-43.",
"title": "Investigation of the relationship between matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 with SARS CoV-2 infections",
"abstract": "<p>SARS-CoV-2 stands for severe acute respiratory syndrome coronavirus 2. Matrix metalloproteinases- 9 (MMP-9) performs a crucial physiological role. In addition to its role in the molecular basis of lung fibrosis, this enzyme may also play a part in the “cytokine storm,” which may represent one of the potential scenarios during coronavirus infection. Tissue inhibitors of metalloproteinase (TIMPs) are well-known for their ability to regulate MMP activity during remodeling of the extracellular matrix. As cytokines, they are also thought of as signaling molecules that impact on a wide range of biological processes. This study aimed to investigate the link between each of MMP-9 and TIMP-1, and COVID19 disease. A total of 58 COVID-19 patients and 30 apparently healthy adults enrolled in this study. The ORF1ab, E and N genes of SARS-CoV-2 were detected using multiplex real-time PCR, while the ELISA technique was used to estimate the level of serum MMP-9, TIMP-1, and C-reactive protein (CRP). The study results demonstrated higher concentrations of MMP-9 in COVID-19 patients (2810 ± 1160 pg/ml) compared to controls (2110 ± 850 pg/ml), with non-significant differences (p=0.002). Unlike, TIMP-1, showed considerably higher levels in the patient’s group (541.53 ± 201.42 pg/ml) than in controls (276.33 ± 67.26 pg/ml) with high significant differences (p ≤ 0.001). Considering this study, TIMP-1 in COVID patients most likely play an important role in inflammatory response. Its clinical utility as a biomarker may be insufficient, but it provides a useful data in the diagnosis of COVID‐19.</p>",
"DOI": "10.5455/jabet.2023.d104",
"views": 1386,
"downloads": 162,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/32/29/178-1654636722-Figure1.jpg",
"caption": "Figure 1. The mean level of MMP-9 (pg\\ml) in COVID-19 patients (2810±1160 pg/ml) and the control group (2110±850 pg/ml) with non-significant differences between the two groups (p=0.002).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/32/29/178-1654636722-Figure2.jpg",
"caption": "Figure 2. The mean level of TIMP-1 (pg\\ml) in COVID-19 patients (541.53±201.42 pg/ml) and control group (276.33±67.26 pg/ml) with highly significant variations between the two groups (p≤0.001).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/32/29/178-1654636722-Figure3.jpg",
"caption": "Figure 3. Pearson correlation analysis of serum MMP-9 versus serum TIMP-1 (r = 0.424, p = 0.001).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/32/29/178-1654636722-Figure4.jpg",
"caption": "Figure 4. The mean level of CRP (pg/ml) in COVID-19 patients (45.12 ± 10.11 pg/ml) and control group (2.5 ± 0.59 pg/ml) with highly significant variations between the two groups (p≤0.001).",
"featured": false
}
],
"authors": [
{
"id": 635,
"affiliation": [
{
"affiliation": "Department of Biology, College of science, University of Baghdad, Baghdad, Iraq."
}
],
"first_name": "May K.",
"family_name": "Ismael",
"email": "majidalbayati2@gmail.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-7662-8038",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "May K. Ismael, Department of Biology, College of science, University of Baghdad, Baghdad, Iraq, e-mail: majidalbayati2@gmail.com",
"article": 150
},
{
"id": 636,
"affiliation": [
{
"affiliation": "Department of Biology, College of science, University of Baghdad, Baghdad, Iraq."
}
],
"first_name": "Lubna M.",
"family_name": "Rasuol",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0001-9836-1151",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 150
},
{
"id": 637,
"affiliation": [
{
"affiliation": "Department of Biology, College of science, University of Baghdad, Baghdad, Iraq."
}
],
"first_name": "Yasir B.",
"family_name": "Qaddoori",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-7380-0565",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 150
}
]
}
]
}
],
"title": "2023",
"slug": "2023",
"order": 6
},
{
"id": 4,
"issues": [
{
"id": 18,
"title": "Vol5 Issue3",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2022",
"slug": "2022-vol5-issue3",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 137,
"slug": "178-1655650827-validation-study-of-c746ag-snp-of-bmpr1b-gene-associated-with-litter-size-in-sheep-genetic-resources-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1655650827",
"published": "2022-08-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/17/178-1655650827.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 712-720.",
"academic_editor": "Hasan-Al-Faruque, PhD; University of Utah, USA.",
"cite_info": "Mony SI, Maruf TM, et al. Validation study of c.746A>G SNP of BMPR1B gene associated with litter size in sheep genetic resources of Bangladesh. J Adv Biotechnol Exp Ther. 2022; 5(3): 712-720.",
"title": "Validation study of c.746A>G SNP of BMPR1B gene associated with litter size in sheep genetic resources of Bangladesh",
"abstract": "<p>The bone morphogenetic protein receptor type 1B (<em>BMPR1B</em>) gene is one of the major fecundity genes that have been investigated in different sheep populations worldwide for its association with prolificacy traits. The present study was performed to validate the association of c.746A>G SNP of <em>BMPR1B</em> gene with litter size trait in different sheep populations of Bangladesh. A total of 192 blood samples were collected from ewes of both farmers’ and institutional flocks comprising sheep populations of Jamuna River Basin (JRB), Barendra (BAT), Coastal (COR), Garole (GAR) and Muzaffarnagari (MUZ). Genotyping of the individuals was performed using PCR-RFLP method and single marker association analysis was carried out to evaluate the relationships between resultant genotypes and litter size trait. The prolificacy attributed homozygous FecB<sup>BB</sup> genotype frequencies were 66, 50 and 55%, respectively in JRB, BAT and GAR populations whereas it was only 0-2% in MUZ and COR populations. Association analysis revealed highly significant <em>(P<0.001)</em> association of litter size trait with genotypes and populations. The mean litter sizes of JRB, BAT, GAR, COR and MUZ were 2.17±0.15, 1.88±0.07 1.90±0.10, 1.12±0.03, and 1.02±0.02, respectively. The prolific JRB, BAT, and GAR ewes produced 0.67 to 0.83 more lambs per lambing than their wild type FecB<sup>++</sup> counterparts. Thus, this study validated the potential contribution of the investigated c.746A>G SNP that could be applied in marker assisted selection (MAS) program for identifying high prolific ewes in order to improve litter size trait at the population level.</p>",
"DOI": "10.5455/jabet.2022.d149",
"views": 1012,
"downloads": 151,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/08/178-1655650827-Figure1.jpg",
"caption": "Figure 1. Image of PCR product of the FecB fragment of BMPR1B gene digested with AvaII restriction enzyme. M = 100bp DNA marker; the wild-type genotype (AA) = 190 bp and homozygous mutant genotype (GG) = 160, 30 bp and heterozygous carrier of FecB mutation (AG) = 190, 160, 30 bp.",
"featured": false
}
],
"authors": [
{
"id": 567,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sabrina Islam",
"family_name": "Mony",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-5363-8687",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 137
},
{
"id": 568,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "T. M.",
"family_name": "Maruf",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-8143-3398",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 137
},
{
"id": 569,
"affiliation": [
{
"affiliation": "Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
}
],
"first_name": "Nure Hasni",
"family_name": "Desha",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0003-3221-249X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 137
},
{
"id": 570,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mohammad Hasanuzzaman",
"family_name": "Pabitra",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0003-1103-5973",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 137
},
{
"id": 571,
"affiliation": [
{
"affiliation": "Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh"
}
],
"first_name": "Sadek",
"family_name": "Ahmed",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-9680-7372",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 137
},
{
"id": 572,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Munir",
"family_name": "Hossain",
"email": null,
"author_order": 6,
"ORCID": "http://orcid.org/0000-0002-8595-7578",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 137
},
{
"id": 573,
"affiliation": [
{
"affiliation": "Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mohammad Shamsul Alam",
"family_name": "Bhuiyan",
"email": "msabhuiyan.abg@bau.edu.bd",
"author_order": 7,
"ORCID": "http://orcid.org/0000-0003-1525-2182",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Shamsul Alam Bhuiyan, Department of Animal Breeding and\r\nGenetics, Bangladesh Agricultural University, Mymensingh, Bangladesh\r\ne-mail: msabhuiyan.abg@bau.edu.bd",
"article": 137
}
]
},
{
"id": 138,
"slug": "178-1646574432-plasma-sex-hormone-binding-globulin-shbg-level-a-comparative-study-between-patients-with-gestational-diabetes-mellitus-and-healthy-pregnant-women",
"type": "short_communication",
"manuscript_id": "178-1646574432",
"published": "2022-08-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/47/178-1646574432.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 721-726.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Mokhtari A, Hasanizadeh R. Plasma sex hormone-binding globulin (SHBG) level: A comparative study between patients with gestational diabetes mellitus and healthy pregnant women. J Adv Biotechnol Exp Ther. 2022; 5(3): 721-726.",
"title": "Plasma sex hormone-binding globulin (SHBG) level: A comparative study between patients with gestational diabetes mellitus and healthy pregnant women",
"abstract": "<p>Gestational diabetes mellitus is a key pregnancy problem worldwide. The aim of this study was to compare the level of globulin binding hormone sex (SHBG) between diabetic pregnant women and healthy pregnant women in Kerman. This descriptive cross-sectional study was performed on diabetic pregnant women where diabetes was confirmed by a 2 h blood glucose tolerance test using 50 g and 100 g glucose. After completion of demographic information and midwifery records blood samples were collected from the patients and the blood glucose levels were measured. Also, SHBG was measured and finally all collected data were analyzed using SPSS software. The serum levels of SHBG were significantly lower in diabetic pregnant women as compared to the control group. However, this level reduction of SHBG in diabetic pregnant women was not significantly associated with other underlying factors and midwifery factors. Therefore, it is suggested that the serum SHBG levels can be used as an early factor in diagnosing the gestational diabetes.</p>",
"DOI": "10.5455/jabet.2022.d150",
"views": 864,
"downloads": 145,
"figures": [],
"authors": [
{
"id": 574,
"affiliation": [
{
"affiliation": "Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Science, & Physiology Research Center, Kerman University of Medical Sciences, Kerman, I.R. Iran"
}
],
"first_name": "Abnoos",
"family_name": "Mokhtari",
"email": "abnoos.mokhtari@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Abnoos Mokhtari, PhD; Kerman Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran, e-mail: abnoos.mokhtari@gmail.com",
"article": 138
},
{
"id": 575,
"affiliation": [
{
"affiliation": "Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Science, & Physiology Research Center, Kerman University of Medical Sciences, Kerman, I.R. Iran"
}
],
"first_name": "Roza",
"family_name": "Hasanizadeh",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 138
}
]
},
{
"id": 133,
"slug": "178-1654441210-association-of-candidate-genes-polymorphisms-in-iraqi-patients-with-chronic-kidney-disease",
"type": "original_article",
"manuscript_id": "178-1654441210",
"published": "2022-08-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/38/178-1654441210.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 687-701",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Darraji MNA, Saqban LH, et al. Association of candidate genes polymorphisms in Iraqi patients with chronic kidney disease. J Adv Biotechnol Exp Ther. 2022; 5(3): 687-701.",
"title": "Association of candidate genes polymorphisms in Iraqi patients with chronic kidney disease",
"abstract": "<p>In many areas of the globe, chronic kidney disease (CKD) is a widespread health concern defined by the progressive loss of kidney function. The genetic contribution to the development of kidney disease is essential in forecasting risk variables and resolving genetic enigmas. Genetic variations are among the factors that might be linked with renal disease development. This research aims to examine the relationship between five single nucleotide polymorphisms (SNPs): rs1126616, rs35068180, rs1800247, rs4236, and rs2248359 with the risk of developing CKD among Iraqi patients. A study involved 80 subjects, divided into fifty CKD patients and thirty healthy subjects. Genotyping was identified by applying the polymerase chain reaction followed by the restriction fragment length polymorphism (PCR-RFLP) method. Compared to the control group, the recurrences of the genotyping TT (p = 0.01) and their allelic T (p = 0.02) in the rs1126616 were considerably higher in the CKD group. Regarding of rs1800247 CT and TT genotypes and (T) allele exhibited a substantial excess in the CKD patients (p = 0.01, 0.0229, < 0.03, respectively). For rs4236, the TT genotype (p = 0.01) and T (p = 0.02) allele were significantly increased in the CKD patients. The recurrences of the genotyping TT (p = 0.02) and their allelic T (p = 0.01) in the rs2248359 were considerably greater in the CKD group. Furthermore, the genotypes and alleles of rs35068180 showed no significant variations among CKD patients and healthy subjects. The results demonstrated that four genetic polymorphisms are probably biomarkers or effective factors linked to CKD patients. Also, specific genetic polymorphisms might lower or raise a patient’s renal disease risk. Differences in genetic and allelic patterns can significantly impact how a disease is treated and what medicines are used.</p>",
"DOI": "10.5455/jabet.2022.d147",
"views": 1094,
"downloads": 192,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/08/178-1654441210-Figure1.jpg",
"caption": "Figure 1. Association between OPN gene rs1126616 and CKD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/08/178-1654441210-Figure2.jpg",
"caption": "Figure 2. Association between OCN gene rs1800247and CKD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/08/178-1654441210-Figure3.jpg",
"caption": "Figure 3. Association between MGP gene rs4236 and CKD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/08/178-1654441210-Figure4.jpg",
"caption": "Figure 4. Association between CYP24A1 gene rs2248359 and CKD.",
"featured": false
}
],
"authors": [
{
"id": 542,
"affiliation": [
{
"affiliation": "Department of Biology, Science College, University of Al-Anbar, Iraq"
}
],
"first_name": "Mustafa Nuhad Al",
"family_name": "Darraji",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-2008-8922",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 133
},
{
"id": 543,
"affiliation": [
{
"affiliation": "Department of Biology, College of Education For Pure Sciences, University of Karbala, Iraq"
}
],
"first_name": "Liqaa Hasson",
"family_name": "Saqban",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 133
},
{
"id": 544,
"affiliation": [
{
"affiliation": "Applied Sciences Department, University of Technology- Iraq, Baghdad, Iraq"
},
{
"affiliation": "MOLTECH Anjou, Universite d’Angers/UMR CNRS 6200, 2, Bd Lavoisier, 49045 Angers, France"
}
],
"first_name": "Mohammed",
"family_name": "Rasheed",
"email": "mohammed.s.rasheed@uotechnology.edu.iq",
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-0768-2142",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammed Rasheed, Applied Sciences Department, University of Technology-Iraq, Baghdad, Iraq, e-mail: mohammed.s.rasheed@uotechnology.edu.iq",
"article": 133
},
{
"id": 545,
"affiliation": [
{
"affiliation": "Department of Biology, College of Education For Pure Sciences, University of Al-Anbar, Iraq"
}
],
"first_name": "Aasim Jasim",
"family_name": "Hussein",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 133
},
{
"id": 546,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Techniques, Al-maarif University College, Al-Anbar, Iraq"
}
],
"first_name": "Thulfiqar Fawwaz",
"family_name": "Mutar",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 133
}
]
},
{
"id": 134,
"slug": "178-1654665867-antioxidant-anticholinesterase-and-neurotrophic-potentials-of-indigenous-medicinal-herbs-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1654665867",
"published": "2022-08-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/00/178-1654665867.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 702-711.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Afrin T, Haque MN, et al. Antioxidant, anticholinesterase, and neurotrophic potentials of indigenous medicinal herbs of Bangladesh. J Adv Biotechnol Exp Ther. 2022; 5(3): 702-711.",
"title": "Antioxidant, anticholinesterase, and neurotrophic potentials of indigenous medicinal herbs of Bangladesh",
"abstract": "<p>In Ayurvedic system of medicine, a variety of medicinal herbs are being prescribed for brain disorders, including mental dysfunction, indifference, and memory impairment. Neuropharmacological mechanisms of these herbs are poorly understood. A total of nineteen indigenous medicinal herbs of Bangladesh were investigated for their neuropharmacological potentials, including antioxidant, anticholinesterase, and neurotrophic activities. The antioxidant activity of plant ethanolic extracts was determined based on their DPPH free radical scavenging capacity. Acetylcholinesterase (AChE) inhibitory activity was determined by the colorimetric assay based on Ellman’s method. The neurotrophic activity of plant extracts was measured based on their capacity to promote neurite outgrowth in a primary culture of hippocampal neurons. Of the herbs, <em>Camellia sinensis</em><em>, Terminalia chebula</em>, <em>Cinnamomum tamala</em><em>, Terminalia bellirica, Phyllanthus emblica, </em>and <em>Curcuma longa</em> exhibited remarkable antioxidant activity with IC<sub>50</sub> values of <100 µg/mL. The highest anticholinesterase activity was shown by <em>C. longa </em>(IC<sub>50 </sub>9.37 µg/mL) followed by <em>C. sinensis</em> (IC<sub>50 </sub>86.01 µg/mL) and <em>C. tamala </em>(IC<sub>50 </sub>86.37 µg/mL). Notably, <em>C. tamala</em> showed the highest neurotrophic activity (an increase in the length of primary neurites by 82% compared to control), whereas <em>C. sinensis</em> and <em>C. longa </em>showed moderate activities. The neurotrophic activity of <em>C. tamala </em>was reported for the first time. The present findings indicate that these indigenous herbs, particularly <em>C. tamala</em>, <em>C. sinensis</em>, and <em>C. longa </em>possess a remarkable neuropharmacological potential and suggest that these neuroactive herbs could be used in disease-modifying therapies for brain disorders.</p>",
"DOI": "10.5455/jabet.2022.d148",
"views": 1408,
"downloads": 165,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/45/25/178-1654665867-Figure1.jpg",
"caption": "Figure 1. Neurotrophic activity of selected medicinal herb extracts. Embryonic rat hippocampal neurons (E19) were cultured on poly-DL-lysine-coated coverslips. Cultures were treated with ethanolic extracts from three medicinal herbs at a defined concentration or vehicle (DMSO) for two days. A) Representative phase-contrast photomicrographs from a culture of the most active concentration of each treatment. Scale bar, 80 μm, applies to all images. B) Morphometric analysis for the number of primary processes (a), the total length of primary process (b), and the length of the longest process (c) demonstrates that extracts promote process/neurite development in a dose-dependent manner. Bars represent the mean ± SEM (n =20 individual neurons). Statistical significance compared to vehicle: *p < 0.05, **p < 0.01 and ***p < 0.001.",
"featured": false
}
],
"authors": [
{
"id": 547,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
},
{
"affiliation": "Interdisciplinary Institute for Food Security, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Tania",
"family_name": "Afrin",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 134
},
{
"id": 548,
"affiliation": [
{
"affiliation": "Dept. of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Patuakhali-8602, Bangladesh"
},
{
"affiliation": "Department of Anatomy, Graduate School of Dongguk University College of Medicine, Gyeongju 38066 Korea"
}
],
"first_name": "Md. Nazmul",
"family_name": "Haque",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 134
},
{
"id": 549,
"affiliation": [
{
"affiliation": "Department of Fishing and Post Harvest Technology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh"
},
{
"affiliation": "Seafood Research Center, Silla University, #605, Advanced Seafood Processing Complex, Wonyang-ro, Amnam-dong, Seo-gu, Busan 49277, Korea"
},
{
"affiliation": "Department of Food Biotechnology, Division of Bioindustry, College of Medical and Life Sciences, Silla University, Busan 46958, Korea"
}
],
"first_name": "Md.",
"family_name": "Mohibbullah",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 134
},
{
"id": 554,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Afzal",
"family_name": "Hossain",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 134
},
{
"id": 555,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Golam",
"family_name": "Mortuza",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 134
},
{
"id": 556,
"affiliation": [
{
"affiliation": "Department of Agronomy, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "A K M Mominul",
"family_name": "Islam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 134
},
{
"id": 557,
"affiliation": [
{
"affiliation": "Department of Anatomy, Graduate School of Dongguk University College of Medicine, Gyeongju 38066 Korea"
}
],
"first_name": "Raju",
"family_name": "Dash",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 134
},
{
"id": 558,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": null,
"author_order": 8,
"ORCID": "http://orcid.org/0000-0003-2911-3255",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 134
},
{
"id": 559,
"affiliation": [
{
"affiliation": "Department of Anatomy, Graduate School of Dongguk University College of Medicine, Gyeongju 38066 Korea"
}
],
"first_name": "Il Soo",
"family_name": "Moon",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 134
},
{
"id": 563,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
},
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "Md. Abdul",
"family_name": "Hannan",
"email": "hannanbmb@bau.edu.bd",
"author_order": 10,
"ORCID": "http://orcid.org/0000-0001-6640-4495",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Abdul Hannan, PhD; Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, e-mail: hannanbmb@bau.edu.bd",
"article": 134
}
]
},
{
"id": 132,
"slug": "178-1655373756-isolation-and-identification-of-salmonella-spp-and-escherichia-coli-from-water-used-during-live-transportation-of-pangasius-catfish-pangasianodon-hypophthalmus",
"type": "original_article",
"manuscript_id": "178-1655373756",
"published": "2022-07-28",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/17/178-1655373756.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 676-686.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Bhuiyan ANMRK, Hossain MM, et al. Isolation and identification of Salmonella spp. and Escherichia coli from water used during live transportation of Pangasius catfish, Pangasianodon hypophthalmus. J Adv Biotechnol Exp Ther. 2022; 5(3): 676-686.",
"title": "Isolation and identification of Salmonella spp. and Escherichia coli from water used during live transportation of Pangasius catfish, Pangasianodon hypophthalmus",
"abstract": "<p>Pangasius catfish (<em>Pangasianodon</em> <em>hypophthalmus</em>) is popular among fish farmers of Bangladesh due to its hardy characteristics, fast growth, and its ability to survive in high densities. Many consumers love to buy this fish, especially as live condition, due to its low market price and delicious fleshy meat. Bacterial outgrowth in transport water is frequent consequence including some enteric groups like <em>Salmonella </em>spp. and <em>E</em>. <em>coli</em>. The study attempted to know the occurrence of <em>Salmonella</em> spp. and <em>E</em>. <em>coli</em> in water used during live transportation of Pangasius catfish in Bangladesh. Water samples were collected from three different Pangasius catfish transportation channels at 2 h interval on-board transportation vehicle. The collected waters were then plated onto SS and EMB agar plates and 15, 20, and 17 suspected isolates were obtained from channel 1, 2 and 3, respectively. The isolates were confirmed through PCR techniques; <em>Salmonella</em> spp. was found only in channel 1 while <em>E</em>. <em>coli</em> were found in all 3 sampling channels under investigation. Among the suspected isolates, 13 isolates were positive for <em>E</em>. <em>coli</em> in channel 1, while 16 in both channel 2 and 3. Among the suspected isolates, 86.54% was <em>E. coli</em> positive, 1.92% was <em>Salmonella</em> positive, and 11.54% isolates were unidentified. The results indicated that the fishes were contaminated with <em>Salmonella </em>spp. and <em>E</em>. <em>coli</em> species either in the culture systems or during handling and live transportation.</p>",
"DOI": "10.5455/jabet.2022.d146",
"views": 1511,
"downloads": 164,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/25/178-1655373756-Figure1.jpg",
"caption": "Figure 1. Map showing the location of sample supply channels of live Pangasius catfish (Pangasianodon hypophthalmus) transportation in Bangladesh. Channel-1: Trishal, Mymensingh to Kawran Bazar, Dhaka; Channel-2: Trishal, Mymensingh to Faridpur; and Channel-3: Trishal, Mymensingh to Poschim Kazir Bazar, Sylhet. The map is extracted from DIVA-GIS using Geographical Information System (GIS) and visualized by ArcMap version 10.7.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/25/178-1655373756-Figure2.jpg",
"caption": "Figure 2. Percentage of Salmonella spp. and E. coli positive. The isolates were obtained from water used during live transportation of Pangasius catfish (Pangasianodon hypophthalmus) in Bangladesh. The arrow showing the amplicon size (211 bp), lane L = 100 bp DNA ladder, lane P = positive control, lane N = negative control, and Lane 1 = positive results (only one isolate was found positive among 52).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/25/178-1655373756-Figure3.jpg",
"caption": "Figure 3. Results of agarose gel electrophoresis of 16S rRNA gene for E. coli confirmation. The isolates were obtained from water used during live transportation of Pangasius catfish (Pangasianodon hypophthalmus) in Bangladesh. The arrow showing the amplicon size (585 bp), lane L = 100 bp DNA ladder, lane P = positive control, and Lane 1-52 = isolates tested for E. coli (45 isolates were found positive among 52).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/25/178-1655373756-Figure4.jpg",
"caption": "Figure 4. Number of Salmonella spp., and E. coli positive isolates considering the total number of isolates obtained from water used during live transportation of Pangasius catfish (Pangasianodon hypophthalmus) in Bangladesh. The water samples were collected from 3 different supply channels.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/10/25/178-1655373756-Figure5.jpg",
"caption": "Figure 5. Percentage of Salmonella spp. and E. coli positive isolates obtained from water used during live transportation of Pangasius catfish (Pangasianodon hypophthalmus) in Bangladesh. The water samples were collected from 3 different supply channels.",
"featured": false
}
],
"authors": [
{
"id": 538,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "A N M Rezvi Kaysar",
"family_name": "Bhuiyan",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-7911-6871",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 132
},
{
"id": 539,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Mubarack",
"family_name": "Hossain",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0001-7275-2429",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 132
},
{
"id": 540,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Naim",
"family_name": "Uddin",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 132
},
{
"id": 541,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Nurul",
"family_name": "Haider",
"email": "raselmnh@bau.edu.bd",
"author_order": 4,
"ORCID": "http://orcid.org/0000-0001-7656-7802",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Nurul Haider, PhD; Department of Fisheries Technology, Bangladesh\r\nAgricultural University, Mymensingh-2202, Bangladesh e-mail: raselmnh@bau.edu.bd",
"article": 132
}
]
},
{
"id": 131,
"slug": "178-1654949612-effect-of-gentamicin-and-doxycycline-on-expression-of-relb-and-rele-genes-in-klebsiella-pneumonia",
"type": "original_article",
"manuscript_id": "178-1654949612",
"published": "2022-07-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/52/178-1654949612.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 667-675.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Sweedan EG , Shehab ZH, et al. Effect of gentamicin and doxycycline on expression of relB and relE genes in Klebsiella pneumonia. J Adv Biotechnol Exp Ther. 2022; 5(3): 667-675.",
"title": "Effect of gentamicin and doxycycline on expression of relB and relE genes in Klebsiella pneumonia",
"abstract": "<p><em>Klebsiella pneumoniae</em> is responsible for a variety of disease in hospitalized patients. The goal of this study was to determine that <em>K. pneumoniae</em> isolates possessed toxin-antitoxin II genes such as <em>relE</em> and <em>relB</em>. Other than that, if there was a correlation between the expression of these two genes and antibiotic resistance in<em> K. pneumoniae. </em>Fifty-seven urine samples were collected from Baghdads’ hospitals; diagnosed and identified by phenotype and biochemical tests and confirmed with VITEK 2 compact system. Only fifteen isolates which were identified as <em>Klebsiella pneumoniae</em>. Antibiotic sensitivity was identified by using twelve antibiotics discs.<em> K. pneumoniae</em> showed 100% resistance to ceftriaxone, amoxicillin, ticarcillin, ticarcillin with clavulanic acid, ceftazidime, tetracycline, while other antibiotics showed less percent of resistant. Minimum inhibitory concentrations (MICs) of antibiotics detected by using macro tube dilution method to identify the antimicrobial activity for <em>K. pneumoniae</em>. The MIC of gentamicin and doxycycline antibiotics was 1024 Mg/ml, 512 Mg/ml, respectively. The <em>relB (</em>115 bp)<em>, </em>and <em>relE (</em>136 pb) genes were detected by polymerase chain reaction. Then gene expression of <em>relB and relE</em> was conducted by using (RT-qPCR) technique treated with sub-MIC concentration of (gentamicin and doxycycline) antibiotics. This study found only ten isolates harbored the two genes. The <em>relB </em>gene expression was increased, but at the same time <em>relE</em> gene expression was decreased compared to control <em>infB1</em> gene expression. This means the bacterial cell tolerance antibiotics sub-MIC concentrations by maintaining the number of bacteria under stress of antibiotics. Finally, these findings suggest the potential of <em>relB</em> to make<em> K. pneumoniae</em> resistant to antibiotics in their infections under antibiotic stress by the toxin-antitoxin II system.</p>",
"DOI": "10.5455/jabet.2022.d145",
"views": 1080,
"downloads": 175,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/40/25/178-1654949612-Figure1.jpg",
"caption": "Figure 1. Results of antibiotic sensitivity test percent for K. pneumoniae isolates 1: Amoxicillin, 2: Cefitrixone, 3: Ticarcillin, 4: Ticarcillin/Clavulanic acid, 5: Ceftazidim, 6: Tetracycline, 7: Ciprofloxcin, 8: Nitrofurantoin, 9: Gentamicin, 10: Doxycycline ,11: Imipenem, 12: Amikacin.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/40/25/178-1654949612-Figure2.jpg",
"caption": "Figure 2. Results of the amplification of relE (136 bp.) on 1.5% agarose gel electrophoresis stained with Ethidium Bromide at 100 volts/Amp for 75 min, M: 100bp Marker.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/40/25/178-1654949612-Figure3.jpg",
"caption": "Figure 3. Results of the amplification of relB (115 bp.) on 1.5% agarose gel electrophoresis stained with Ethidium Bromide at 100 volts/Amp for 75 min, M: 100bp Marker.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/40/25/178-1654949612-Figure4.jpg",
"caption": "Figure 4. Ct value of infB1 in K. pneumoniae for treated and without treated with sub-MIC of antibiotics (doxycycline and gentamicin) as control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/40/25/178-1654949612-Figure5.jpg",
"caption": "Figure 5. Ct value of relB in K. pneumoniae for treated and without treated with sub-MIC of antibiotics (doxycycline and gentamicin).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/40/25/178-1654949612-Figure6.jpg",
"caption": "Figure 6. Ct value of relE in K. pneumoniae for treated and without treated with sub-MIC of antibiotics (doxycycline and gentamicin).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/40/25/178-1654949612-Figure7.jpg",
"caption": "Figure 7. Fold changes in expression of relB and relE in K. pneumoniae isolate which treated with sub-MIC (doxycycline and gentamicin) antibiotics.",
"featured": false
}
],
"authors": [
{
"id": 535,
"affiliation": [
{
"affiliation": "Biology Department, College of Science, University of Baghdad, Baghdad, Iraq"
}
],
"first_name": "Enass Ghassan",
"family_name": "Sweedan",
"email": "enassghassan1@gmail.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-7330-9939",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Enass Ghassan Sweedan, PhD; Biology Department, College of Science, University of Baghdad, Baghdad, Iraq, e-mail: enassghassan1@gmail.com",
"article": 131
},
{
"id": 536,
"affiliation": [
{
"affiliation": "Biology Department, College of Science for Women, University of Baghdad, Baghdad, Iraq"
}
],
"first_name": "Zina Hashem",
"family_name": "Shehab",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 131
},
{
"id": 537,
"affiliation": [
{
"affiliation": "Biology Department, College of Science, University of Baghdad, Baghdad, Iraq"
}
],
"first_name": "May Talib",
"family_name": "Flayyih",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 131
}
]
},
{
"id": 126,
"slug": "178-1654118747-evaluation-of-the-thrombolytic-activity-of-the-methanolic-extract-of-melia-azedarach-fruits-and-leaves-in-vitro",
"type": "original_article",
"manuscript_id": "178-1654118747",
"published": "2022-07-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/28/178-1654118747.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 634-641.",
"academic_editor": "Akhi Moni, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Hussain HG, Agha MH. Evaluation of the thrombolytic activity of the methanolic extract of Melia azedarach fruits and leaves in vitro. J Adv Biotechnol Exp Ther. 2022; 5(3): 634-641.",
"title": "Evaluation of the thrombolytic activity of the methanolic extract of Melia azedarach fruits and leaves in vitro",
"abstract": "<p><em>Melia azedarach</em> is a plant of the Meliaceae family, used worldwide in various medical fields. Because of the widespread incidence of thrombosis worldwide, especially during the coronavirus epidemic, this study was conducted to evaluate the in vitro thrombolytic effect of methanolic extracts of <em>Melia azedarach</em> leaves and fruits. Series of dilutions starting from 2 mg/ml to 20mg/ml were prepared from the methanolic extracts. The results showed that thrombolysis rates were between (18.7%-29.3%) for fruit extract in distilled water, (18.3%-30.1%) in phosphate buffer, (19.3%-35%) for leaves extract in distilled water and (20%-32.3%) in phosphate buffer for dilutions from 2 mg/ml to 20mg/ml. The streptokinase (positive control) had a thrombolytic effect of 47.54 % in distilled water and 44.36 % in phosphate buffer, compared to negative controls of 5.94 % and 6.34 %, respectively. Phytochemical screening found flavonoids and coumarins in leaves extract and only flavonoids in fruit extract, suggesting that the increase in thrombolytic effect may be attributed to these flavonoids and coumarins. The total phenolic content was 15.78 (mg GAE\\1g) in leaves extract and 3.64 (mg GAE\\1g) in fruit extract, while the total flavonoid content was 0.813 (mg QE\\1g) in leaves extract and 0.17 (mg QE\\1g) in fruit extract. In conclusion, these results showed that <em>Melia azedarach</em> has a thrombolytic effect.</p>",
"DOI": "doi.org/10.5455/jabet.2022.d142",
"views": 876,
"downloads": 206,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/29/26/178-1654118747-Figure1.jpg",
"caption": "Figure 1. The thrombolytic activity (%) according to concentration of fruit extract (a) and leaves extract (b) in distilled water (DW). The thrombolytic activity % according to concentration of fruit extract (c) and leaves extract (d) in phosphate buffer (PW). *** indicates P values<0.001 compared between the thrombolytic activity of SK and 20 mg/ml of each extract.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/29/26/178-1654118747-Figure2.jpg",
"caption": "Figure 2 Comparison between the highest thrombolytic effect for all extracts with positive and negative controls.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/29/26/178-1654118747-Figure3.jpg",
"caption": "Figure 3. Standard curve of gallic acid from which the concentration of phenols was calculated.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/29/26/178-1654118747-Figure4.jpg",
"caption": "Figure 4. Standard curve of quercetin from which the concentration of flavonoids was calculated.",
"featured": false
}
],
"authors": [
{
"id": 507,
"affiliation": [
{
"affiliation": "Department of pharmacognosy and medicinal plant, Faculty of pharmacy, Damascus University, Damascus, Syria."
}
],
"first_name": "Hiba Ghanem",
"family_name": "Hussain",
"email": null,
"author_order": 1,
"ORCID": "http://hiba56304@gmail.com",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Hiba Ghanem Hussain, Department of pharmacognosy and medicinal plant, Faculty of pharmacy, Damascus University, Damascus, Syria, e-mail: hiba56304@gmail.com",
"article": 126
},
{
"id": 508,
"affiliation": [
{
"affiliation": "Department of pharmacognosy and medicinal plant, Faculty of pharmacy, Damascus University, Damascus, Syria."
}
],
"first_name": "Mohammed Isam Hasan",
"family_name": "Agha",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 126
}
]
},
{
"id": 125,
"slug": "178-1655357396-effect-of-growth-factors-present-in-serum-on-insulin-resistance-and-endothelial-dysfunction-in-eahy926-cells",
"type": "original_article",
"manuscript_id": "178-1655357396",
"published": "2022-07-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/12/178-1655357396.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 623-633.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Maganti H, Thandapani P, et al. Effect of growth factors present in serum on insulin resistance and endothelial dysfunction in EA.hy926 cells. J Adv Biotechnol Exp Ther. 2022; 5(3): 623-633.",
"title": "Effect of growth factors present in serum on insulin resistance and endothelial dysfunction in EA.hy926 cells",
"abstract": "<p>Insulin resistance is a pathophysiological function of Type II diabetes mellitus which can be comprehended by quantifying the parameters critical to the insulin signaling pathway. Serum has a profound role in evaluating cellular growth and metabolism <em>in vitro</em>. We hypothesize that the growth factors present in serum such as IGF, EGF, and FGF have an effect on the regulatory components of the insulin signaling pathway leading to insulin resistance. This study focuses on the metabolic effect of Fetal Bovine Serum (FBS) in endothelial (EA.hy926) cells. A dose and time-dependent treatment of FBS on the chosen cells was followed by assessing cell viability and glucose uptake capacity using MTT and 2-NBDG assays respectively. Spectrophotometric analysis of nitric oxide (NO) and lactate dehydrogenase (LDH) determined vascular homeostasis and no cytotoxic effects of the serum, respectively, in endothelial cells. These findings indicate that FBS at higher levels could possibly lead to loss of NO activity which in turn could impair endothelium-mediated dilation. The inhibition of the enzymatic activity of eNOS, which in response to the stress may activate the release of LDH in endothelial cells displaying cytotoxicity. In conclusion, our findings indicate that a specific concentration of serum exposure enhances insulin signaling and endothelium cell regulation by modulating glucose uptake and NO production.</p>",
"DOI": "doi.org/10.5455/jabet.2022.d141",
"views": 900,
"downloads": 183,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/39/25/178-1655357396-Figure1.jpg",
"caption": "Figure 1. Assessment of cell viability by MTT in endothelial cells after treatment with fetal bovine serum in a time (5 min, 10min, 15min) and dose (5%, 10%, 15%) dependent manner. Triton X-100 (0.01%) is a positive control and DMSO serves as solvent control. Data represents the mean ± SEM of triplicates (N=3). #p < 0.001 as compared to control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/39/25/178-1655357396-Figure2.jpg",
"caption": "Figure 2. Nitric Oxide estimation in endothelial cells after treatment with fetal bovine serum in a time (5 min, 10min, 15min) and dose (5%, 10%, 15%) dependent manner, by using NED and sulphanilamide reagents. L-NAME is a positive control. Data represents the mean ± SEM of triplicates (N=3). *p < 0.05 and #p < 0.001 as compared to control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/39/25/178-1655357396-Figure3.jpg",
"caption": "Figure 3. LDH estimation in endothelial cells to observe cell cytotoxicity after treatment with fetal bovine serum in a time (5 min, 10min, 15min) and dose (5%, 10%, 15%) dependent manner. Triton X-100 (0.01%) is the positive control. Data represents the mean ± SEM of triplicates (N=3). #p < 0.001 as compared to control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/39/25/178-1655357396-Figure4.jpg",
"caption": "Figure 4. Estimation of percentage of glucose uptake by 2-NBDG which is a fluorescent analogue of glucose, in endothelial cells after treatment with fetal bovine serum in a time (5 min, 10min, 15min) and dose (5%, 10%, 15%) dependent manner. Insulin (100nM) is a positive control and DMSO (0.01%) serves as solvent control. Data represents the mean ± SEM of triplicates (N=3). *p < 0.05 as compared to control.",
"featured": false
}
],
"authors": [
{
"id": 500,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Harika",
"family_name": "Maganti",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 125
},
{
"id": 501,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Prabu",
"family_name": "Thandapani",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 125
},
{
"id": 502,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Ragulprasath",
"family_name": "Kailasam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 125
},
{
"id": 503,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Adithi",
"family_name": "Pisapati",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 125
},
{
"id": 504,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Akshaya",
"family_name": "Bala",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 125
},
{
"id": 505,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Angel",
"family_name": "Mendonca",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 125
},
{
"id": 506,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603 203, Tamil Nadu, India"
}
],
"first_name": "Sujatha",
"family_name": "Sundaresan",
"email": "sujathas1@srmist.edu.in",
"author_order": 7,
"ORCID": "http://orcid.org/0000-0001-7676-2130",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sujatha Sundaresan, PhD; Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India, e-mail: sujathas1@srmist.edu.in",
"article": 125
}
]
},
{
"id": 127,
"slug": "178-1652146042-efficacy-of-soil-borne-burkholderia-cepacia-like-mb-01-strain-for-shobicron-and-vertimec-degradation",
"type": "original_article",
"manuscript_id": "178-1652146042",
"published": "2022-07-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/57/178-1652146042.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 642-653.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Moniruzzaman M, Jinnah MM, et al. Efficacy of soil-borne Burkholderia Cepacia-like MB-01 strain for shobicron and vertimec degradation. J Adv Biotechnol Exp Ther. 2022; 5(3): 642-653.",
"title": "Efficacy of soil-borne Burkholderia Cepacia-like MB-01 strain for shobicron and vertimec degradation",
"abstract": "<p>Pesticides are necessary for agriculture, yet their highly toxic ingredients harm the ecosystem. Due to their toxicity, uncontrolled releases of large quantities of pesticides pollute the environment and provide a larger health risk to plants, animals, and humans. Bacteria are capable of degrading such pollutants and saving our ecosystem. In this study, a bacterial strain was isolated from Shobicron and Vertimec-treated lady’s finger soil using enrichment culture. The strain was identified as <em>Burkholderia Cepacia</em>-Like MB-01 based on morphological, physiological, and biochemical traits, as well as phylogenetic analysis of the 16S rRNA sequence. The bacterium grew best at 35 °C with a pH of 7. Furthermore, it was susceptible (S) to Cefepime and Penicillin but intermediate (I) resistant to Carbapenem and Tetracycline and resistant (R) to Ciprofloxacin, Kanamycin, and Gentamycin in an antibiotic sensitivity test. The rate of shobicron and vertimec degradation was measured over a five-day period using Mineral Salt (MS) medium. In its optimum growth condition, shobicron and vertimec degradation rates were around 76 % and 80 %, respectively. The isolated bacterial strain was capable of detoxifying shobicron and vertimec in the experiment. As a result, the bacterial strain could exploit as a possible shobicron and vertimec degrader for pesticide bioremediation.</p>",
"DOI": "10.5455/jabet.2022.d143",
"views": 1093,
"downloads": 104,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/26/178-1652146042-Figure3.jpg",
"caption": "Figure 3. Optimization of pH and temperature. (a) Effect of pH on growth of the strain MB-01, where the maximum growth was obtained at pH 7; (b) Effect of temperature on bacterial growth, where maximum growth was observed at 35 °C. Data were recorded after 12 hours of incubation.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/26/178-1652146042-Figure4.jpg",
"caption": "Figure 4. Antibiotic sensitivity test of the strain MB-01. Isolate was resistant to most of the tested antibiotics, and susceptible to Cefepime, and Penicillin. Note: Resistant=<10 mm; Intermediate resistance=10-15 mm; Susceptible=>15 mm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/26/178-1652146042-Figure5.jpg",
"caption": "Figure 5. Showing of degradation of shobicron (a) and vertimec (b) by the strain MB-01 in its optimum conditions. Data were recoded up to 5 days of incubation.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/26/178-1652146042-Figure6.jpg",
"caption": "Figure 6. Effect of pH (a) and temperature (b) on shobicron and vertimec degradation by the strain MB-01. Data were recorded after 5 days of incubation.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/26/178-1652146042-Figure1.jpg",
"caption": "Figure 1. Isolated shobicrn and vertimec degrading bacterial strain. (a) Bacterial growth on MS agar plate supplemented with 4 µg/ml of shobicron. (b) Gram staining isolated strain indicating gram-negative and rod-shaped structure.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/26/178-1652146042-Figure2.jpg",
"caption": "Figure 2. Phylogenetic tree analysis with similar bacterial strains. The tree was constructed by the neighbor-joining method based on 16S rRNA gene sequences of strain MB-01 and related Burkholderia type strain.",
"featured": false
}
],
"authors": [
{
"id": 511,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Md.",
"family_name": "Moniruzzaman",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 127
},
{
"id": 512,
"affiliation": [
{
"affiliation": "Department of Mathematics and Natural Sciences, Brac University, Dhaka- 1212, Bangladesh"
}
],
"first_name": "Mst. Maskera",
"family_name": "Jinnah",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 127
},
{
"id": 513,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Most. Merina",
"family_name": "Mostari",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 127
},
{
"id": 514,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Shirmin",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 127
},
{
"id": 515,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Md. Joy",
"family_name": "Pramanik",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 127
},
{
"id": 516,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Jui",
"family_name": "Biswas",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 127
},
{
"id": 517,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Al",
"family_name": "Imran",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 127
},
{
"id": 518,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Md. Salah",
"family_name": "Uddin",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 127
},
{
"id": 519,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Md. Abu",
"family_name": "Saleh",
"email": "saleh@ru.ac.bd",
"author_order": 9,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Abu Saleh, PhD; Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh, e-mail: saleh@ru.ac.bd",
"article": 127
},
{
"id": 520,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh"
}
],
"first_name": "Shahriar",
"family_name": "Zaman",
"email": "szaman@ru.ac.bd",
"author_order": 10,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Shahriar Zaman, Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi- 6205, Bangladesh,\r\ne-mail: szaman@ru.ac.bd",
"article": 127
}
]
},
{
"id": 129,
"slug": "178-1650449385-molecular-characterization-and-antibacterial-activities-of-mangrove-endophytic-fungi-from-coastal-kenya",
"type": "original_article",
"manuscript_id": "178-1650449385",
"published": "2022-07-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/33/178-1650449385.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 654-666.",
"academic_editor": "Akhi Moni, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Kiti HM, Kibiti CM, et al. Molecular characterization and antibacterial activities of mangrove endophytic fungi from coastal Kenya. J Adv Biotechnol Exp Ther. 2022; 5(3): 654-666.",
"title": "Molecular characterization and antibacterial activities of mangrove endophytic fungi from coastal Kenya",
"abstract": "<p>The advent of resistant pathogenic bacteria and fungi across the globe is threatening the efficacy of antibiotic drugs. Thus, microbial infections are becoming a threat to life. Endophytic fungi remain a viable source of secondary metabolites with unique spectra of biological activities. This study isolated and characterized endophytic fungi from selected mangrove species of coastal Kenya and further ascertained their activities. A total of 18 fungal endophytes selected from mangrove species were investigated for antimicrobial activity against gram-positive <em>Staphylococcus aureus </em>and gram-negative <em>Escherichia coli</em>. Potato dextrose agar and potato dextrose broth were used for isolation, purification, and fermentation at 28<sup>o</sup>C for 7–15 days. Extraction of fungal metabolites was achieved using ethyl acetate (1:1 v/v) and ethyl acetate in 10% methanol (9:1 v/v). Solvents were recovered in a fume hood and extracts were dissolved in 1 ml of dimethyl sulfoxide. Molecular characterization completely identified 9 species, namely: <em>Aspergillus</em> <em>flavus, Aspergillus niger, Aspergillus tubingensis, Aspergillus oryzae, Rhizophora nomius, Aspergillus awamori, Aspergillus aculeatus, Aspergillus bravionivious, and Aspergillus welwitchiae. </em>The minimum inhibitory concentration of ethyl acetate crude extracts of the most active fungal isolate, <em>A. flavus </em>(MT447532.1), was 0.91 ± 0.05 mg/ml and 0.82 ± 0.052 mg/ml against <em>S. aureus </em>and <em>E. coli</em>, respectively. Results showed that some crude extracts of mangrove fungal endophytes from coastal Kenya are effective against bacteria, hence a promising source of novel organic natural metabolites with a possible wide range of biological activities.</p>",
"DOI": "doi.org/10.5455/jabet.2022.d144",
"views": 1178,
"downloads": 150,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/03/25/178-1650449385-Figure1.jpg",
"caption": "Figure 1. A map of the Kenya coast showing mangrove forests of Gazi Bay, Tudor and Mida creeks from where mangrove twig samples were collected [14].",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/03/25/178-1650449385-Figure2.jpg",
"caption": "Figure 2. PCR Amplicons (approximately 500 base pairs) of ITS region of endophytic fungi isolated from selected mangrove species of coastal Kenya.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/03/25/178-1650449385-Figure3.jpg",
"caption": "Figure 3. Phylogenetic tree of 9 unique fungal isolates rooted from Fusarium oxysporum from coastal Kenya.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/03/25/178-1650449385-Figure4.jpg",
"caption": "Figure 4. Mean inhibition diameters of ethyl acetate crude extracts against Staphylococcus aureus, and Escherichia coli.",
"featured": false
}
],
"authors": [
{
"id": 527,
"affiliation": [
{
"affiliation": "School of Applied and Health Sciences, Technical University of Mombasa, P.O. Box 90420 – 80100, Mombasa, Kenya"
}
],
"first_name": "Helen M.",
"family_name": "Kiti",
"email": "kitihelenmwaka@tum.ac.ke",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-5287-3482",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Helen M. Kiti, PhD; School of Applied and Health Sciences, Technical University of Mombasa, Mombasa, Kenya e-mail: kitihelenmwaka@tum.ac.ke",
"article": 129
},
{
"id": 528,
"affiliation": [
{
"affiliation": "School of Applied and Health Sciences, Technical University of Mombasa, P.O. Box 90420 – 80100, Mombasa, Kenya"
}
],
"first_name": "Cromwell M.",
"family_name": "Kibiti",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-3611-4427",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 129
},
{
"id": 529,
"affiliation": [
{
"affiliation": "School of Applied and Health Sciences, Technical University of Mombasa, P.O. Box 90420 – 80100, Mombasa, Kenya"
}
],
"first_name": "Cosmas N.",
"family_name": "Munga",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 129
},
{
"id": 530,
"affiliation": [
{
"affiliation": "School of Applied and Health Sciences, Technical University of Mombasa, P.O. Box 90420 – 80100, Mombasa, Kenya"
}
],
"first_name": "Josiah O.",
"family_name": "Odalo",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0002-4032-4312",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 129
},
{
"id": 531,
"affiliation": [
{
"affiliation": "Department of Chemistry, Pwani University, P.O. Box 195-80108, Kilifi, Kenya"
}
],
"first_name": "Paul M.",
"family_name": "Guyo",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-3444-7973",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 129
},
{
"id": 532,
"affiliation": [
{
"affiliation": "Kenya Marine and Fisheries Research Institute, P.O Box 81651-80100, Mombasa, Kenya"
}
],
"first_name": "Samuel M.",
"family_name": "Mwamburi",
"email": null,
"author_order": 6,
"ORCID": "http://orcid.org/0000-0003-4253-3967",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 129
}
]
},
{
"id": 122,
"slug": "178-1652412212-spatial-distribution-of-phytoplankton-in-lasolo-bay-of-southeast-sulawesi-province-indonesia",
"type": "original_article",
"manuscript_id": "178-1652412212",
"published": "2022-07-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/35/178-1652412212.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 615-622.",
"academic_editor": "Akhi Moni, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Irawati M, Sara L, et al. Spatial distribution of phytoplankton in Lasolo Bay of Southeast Sulawesi province, Indonesia. J Adv Biotechnol Exp Ther. 2022; 5(3): 615-622.",
"title": "Spatial distribution of phytoplankton in Lasolo Bay of Southeast Sulawesi province, Indonesia",
"abstract": "<p>This study aimed to analyze the distribution of phytoplankton abundance and its relationship with water quality in Lasolo Bay waters. The study was conducted during the West season 2020. The sample consisted of 9 stations located near the mouth of the Lasolo River (station A), coastal waters (station B) and the center part of Lasolo Bay (station C). Phytoplankton sampling was using plankton nets with 25 µm mesh size. Identification of phytoplankton used microscope and the abundance calculation by using Sedgwick Rafter Counting Cell. The results showed that there are 23 phytoplankton genera from 5 classes found, namely 12 genera of <em>Bacillariophycea</em> class, 5 genera of <em>Dinophyceae</em> class, 3 genera of <em>Chlorophyceae</em> class, 2 genera of <em>Cyanophyceae</em> class and 1 genera of <em>Coscinodiscophyceae</em> class. By abundance, phytoplankton compositions of the class <em>Bacillariophyceae</em> predominate with a percentage of 57%. The type of phytoplankton that has the highest abundance is <em>Nitzschia</em> sp. of 2,407 cells/l and the lowest abundance of <em>Amphidium </em>sp. of 1,368 cells/l. Phytoplankton abundance at station A is 41,093 cells/l, station B is 14,234 cells/l and station C is 14,735 cells/l. The distribution of phytoplankton abundance is also influenced by physical and chemical factors such as turbidity, TSS, Nitrate, and Phosphate.</p>",
"DOI": "doi.org/10.5455/jabet.2022.d140",
"views": 525,
"downloads": 112,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/29/26/178-1652412212-Figure1.jpg",
"caption": "Figure 1. Lasolo Bay location map.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/30/26/178-1652412212-Figure2.jpg",
"caption": "Figure 2. (a) Phytoplankton class composition; (b) Abundance of phytoplankton.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/30/26/178-1652412212-Figure3.jpg",
"caption": "Figure 3. Graph of phytoplankton abundance at stations A, B, and C.",
"featured": false
}
],
"authors": [
{
"id": 492,
"affiliation": [
{
"affiliation": "Doctoral Student of Agricultural Science, Postgraduate Program, Halu Oleo University, Kendari, Southeast Sulawesi, Indonesia"
}
],
"first_name": "Irawati",
"family_name": "M",
"email": "irawatijumaidin@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Irawati, Agricultural Science, Postgraduate Program, Halu Oleo University,\r\nKendari, Southeast Sulawesi, Indonesia, e-mail: irawatijumaidin@gmail.com",
"article": 122
},
{
"id": 493,
"affiliation": [
{
"affiliation": "Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Halu Oleo University, Kendari, Southeast Sulawesi,Indonesia"
}
],
"first_name": "La",
"family_name": "Sara",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 122
},
{
"id": 494,
"affiliation": [
{
"affiliation": "Department of Geological Engineering, Halu Oleo University, Kendari, Southeast Sulawesi, Indonesia"
}
],
"first_name": "Muliddin",
"family_name": "M",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 122
},
{
"id": 495,
"affiliation": [
{
"affiliation": "Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Halu Oleo University, Kendari, Southeast Sulawesi, Indonesia"
}
],
"first_name": "Asriyana",
"family_name": "M",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 122
}
]
},
{
"id": 119,
"slug": "178-1652598688-codon-usage-bias-and-purifying-selection-identified-in-cirrhinus-reba-mitogenome",
"type": "original_article",
"manuscript_id": "178-1652598688",
"published": "2022-07-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/12/178-1652598688.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 605-614.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Islam MN, Sultana S. Codon usage bias and purifying selection identified in Cirrhinus reba mitogenome. J Adv Biotechnol Exp Ther. 2022; 5(3): 605-614.",
"title": "Codon usage bias and purifying selection identified in Cirrhinus reba mitogenome",
"abstract": "<p>In our previous study, we described sequence assembly and organization of the complete mitochondrial genome of a threatened labeonine fish, <em>Cirrhinus reba</em> (GenBank accession no.: MN862482). In this study, our attempts were to find out any mutation or selection pressures and codon usage patterns existing in the mitogenome of the same fish. We applied bioinformatics tools to measure important gene parameters including AT/GC skewness, codon adaptation index (CAI), the effective number of codons (ENc) and GC percentages of each protein coding gene. We found an overrepresentation of A and C resulting a lower number of T and G bases, respectively, where AT-skew was slightly positive, and GC-skew was slightly negative. Except for ND6, all protein coding genes (PCGs) had negative GC-skew, which indicated the higher occurrence of Cs. Based on comparative selective strength analysis with the PCGs of two related mitogenomes, we revealed that most of the PCGs (except for ND1, COX2 and ND5 genes) retained the dN/dS or Ka/Ks values less than 0.1 implying that they evolved under strong purifying selection. We further analyzed the codon frequency and relative synonymous codon usage (RSCU) and observed a total of 3802 codons which were used for coding 20 amino acids by a standard set of 64 codons. The amino acids Leucine and Serine were encoded each by six different codons, whereas rest of the amino acids was encoded by either two or four codons. We identified a total of 25 RSCU values (> 1) and revealed 12 codons as “overpresented” that implied for codon usage bias to engage in highly expressed genes for efficient protein synthesis via translational selection. The existence of codon usage biasness rolling in translational selection and the signs of purifying selection identified in PCGs suggest obvious conservation of this threatened fish species.</p>",
"DOI": "10.5455/jabet.2022.d139",
"views": 1413,
"downloads": 241,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/26/178-1652598688-Figure1.jpg",
"caption": "Figure 1. Graphical presentation of AT- and GC skew of 13 PCGs of Cirrhinus reba.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/26/178-1652598688-Figure2.jpg",
"caption": "Figure 2. Percentages of GC content at the 1st, 2nd and 3rd position of codons of 13 PCGs of Cirrhinus reba.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/26/178-1652598688-Figure3.jpg",
"caption": "Figure 3. Codon usage frequency (A) and relative synonymous codon usage (RSCU) (B) of the mitochondrial genome of Cirrhinus reba (Reverse complementary sequence was used for ND6 gene which was encoded on the L-strand and incomplete stop codons were not included).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/53/26/178-1652598688-Figure4.jpg",
"caption": "Figure 4. Selective strength of protein coding genes of Cirrhinus reba (CR) mitogenome comparing to that of Labeo bata (LB) (GenBank accession no.: AP011198.1) and Labeo calbasu (LC) (GenBank accession no: AP012143.1). The ratio indicates the rate of non-synonymous substitutions to the rate of synonymous substitutions (Ka/Ks) for each protein coding genes.",
"featured": false
}
],
"authors": [
{
"id": 478,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh"
}
],
"first_name": "Mohammad Nazrul",
"family_name": "Islam",
"email": "nazrul.sau.bd@gmail.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-5692-3566",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Nazrul Islam, PhD; Department of Biotechnology, Shere Bangla Agricultural University, Sher-e-Bangla Nagar, Bangladesh, e-mail: nazrul.sau.bd@gmail.com",
"article": 119
},
{
"id": 479,
"affiliation": [
{
"affiliation": "Fisheries Biotechnology Division, National Institute of Biotechnology, Dhaka-1349, Bangladesh"
}
],
"first_name": "Shirin",
"family_name": "Sultana",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 119
}
]
},
{
"id": 118,
"slug": "178-1653458316-protective-effect-of-l-carnitine-nanoparticles-vs-carnitine-on-lead-acetate-induced-toxicity-in-male-rats",
"type": "original_article",
"manuscript_id": "178-1653458316",
"published": "2022-07-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/22/178-1653458316.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 590-604.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Mahdi HT, Ghadhban RF, Protective effect of L-carnitine nanoparticles Vs carnitine on lead acetate-induced toxicity in male rats. J Adv Biotechnol Exp Ther. 2022; 5(3): 590-604.",
"title": "Protective effect of L-carnitine nanoparticles Vs carnitine on lead acetate-induced toxicity in male rats",
"abstract": "<p>Chitosan nanoparticles are important materials that are widely used in many biological, engineering and food industries and are also used as plant growth stimulants as well as use as vectors for drug delivery to target cells. Whereas L-carnitine (LC) is a water-soluble compound that contributes to the transport of long-chain fatty acids across the mitochondrial membranes and the oxidation of β-lipids. 60 male rats (Rattus Rattus) were divided into six equal groups. The first group (control group) received orally distilled water. The second group received 1ml lead acetate orally at a dose of 30 mg/kg of body weight daily for 30 days. Third group received 1ml lead acetate (30mg/kg B.W) + L-carnitine (100mg/ kg B.W. /daily). The fourth group received 1ml lead acetate (30mg/kg B.W.) + Nano L-carnitine (100mg/ kg B.W./ daily). The fifth group received 1ml of L-Carnitine orally at a dose of 100mg/ kg B.W /daily. The sixth group received 1ml of L-Carnitine-NPs orally at 100mg/ kg B.W /daily. Our findings demonstrated that exposure to lead acetate caused a significant increase in liver enzymes aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) and renal function (creatinine and urea) in the lead acetate group. Whereas lead treatment increased oxidative stress and reactive oxygen species (ROS). Histopathological study showed significant changes in the brain (cerebellum) that disrupted the normal arrangement of the three layers, with large distances between the Purkinje cell layer and the molecular or granular layer. According to the study, we can conclude that the Nano L-Carnitine had a greater role in protecting against the effect of lead at the hematological parameters and a clear role in the protection against histopathology change of lead poisoning. L-Carnitine and Nano L-Carnitine had an active role in protecting against lead acetate toxicity.</p>",
"DOI": "10.5455/jabet.2022.d138",
"views": 1691,
"downloads": 205,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/26/178-1653458316-Figure1.jpg",
"caption": "Figure 1. Synthesis of chitosan nanoparticles.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/26/178-1653458316-Figure2.jpg",
"caption": "Figure 2. Scanning electron microscopy (SEM) shows the morphology and size of biogenic nanoparticles (LC-NPs).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/26/178-1653458316-Figure3.jpg",
"caption": "Figure 3. (A) The Cerebellum section of rats in the control group (hematoxylin and Eosin .100x) showed no histopathological changes, which is composed of the outer molecular layer, inner granular layer cells, and in between, the single layer of Purkinje cells. (B) Cerebellum section of lead acetate treated rats. Shows disrupt the normal arrangement of three layers; large spaces between Purkinje's cell layer and a molecular layer or granular layer were seen. The treated rats were administrated 30mg/kg body weight of lead acetate (hematoxylin and Eosin .400x). (C) Cerebellum Sections of Third Group (lead acetate and L-Carnitine). show normal Purkinje cells and normal arrangement of cerebellum layers. (D) Cerebellum Sections of the fourth Group (lead acetate and Nano L-Carnitine). Shows normal Purkinje cells and normal arrangement of cerebellum layers. (E) Cerebellum Sections of the fifth Group (L-Carnitine at a dose of 100mg/ kg B.W /daily for two months). Shows normal architecture, normal layers, and presence in high density central to Purkinje cells. (F) Cerebellum Sections of the sixth Group (Nano L-Carnitine at a dose of 100mg/ kg B.W /daily for two months). showed normal architecture, and normal layers of the cerebellum.",
"featured": false
}
],
"authors": [
{
"id": 476,
"affiliation": [
{
"affiliation": "Department of Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Basrah, Basrah, Iraq"
}
],
"first_name": "Hayder Talib",
"family_name": "Mahdi",
"email": "hayder@alameed.edu.iq",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Hayder Talib Mahdi, PhD; Department of Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Basrah, Basrah, Iraq, e-mail: hayder@alameed.edu.iq",
"article": 118
},
{
"id": 477,
"affiliation": [
{
"affiliation": "Department of Physiology, college of medicine, University of Al-Ameed, Karbala, Iraq"
}
],
"first_name": "Rashad Fadhil",
"family_name": "Ghadhban",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 118
}
]
},
{
"id": 116,
"slug": "178-1651419347-granzyme-b-gene-polymorphisms-and-risk-of-hepatocellular-carcinoma-in-patients-with-chronic-hepatitis",
"type": "original_article",
"manuscript_id": "178-1651419347",
"published": "2022-07-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/07/178-1651419347.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 577-589.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Alshamary HS, Mayah QSA, et al. Granzyme B gene polymorphisms and risk of hepatocellular carcinoma in patients with chronic hepatitis. J Adv Biotechnol Exp Ther. 2022; 5(3): 577-589.",
"title": "Granzyme B gene polymorphisms and risk of hepatocellular carcinoma in patients with chronic hepatitis",
"abstract": "<p>Infection with the hepatitis B virus (HBV) continues to be a hazard for public health across the globe. Chronic hepatitis, cirrhosis, and hepatocellular carcinoma (HCC) are all possible outcomes. It is obvious that certain patients with chronic hepatitis B (CHB) viral infection developed HCC, while other under almost similar circumstances do not. The present study aimed to investigate the possible a link between three single nucleotide gene polymorphisms (SNPs) in GzmB genes with the development of HCC. A total of 85 patients diagnosed with CHB participated in this research (40 patients with HCC and 45 patients without HCC). Three SNPs in GzmB gene (rs7144366, rs8192917 and rs2236338) were genotyped using restriction fragment length polymorphism (RFLP). The haplotype blocks derived from the three SNPs were assembled, and the linkage disequilibrium (LD) between the SNPs was determined using the SHEsis software. The homozygous mutant genotype (CC) was shown to be significantly more common in patients with HCC (27.5 %) than in those without HCC (11.11 %) (OR= 3.93, 95% CI=1.13-13.62, p=0.031). At allelic level, the mutant allele (C) was more frequent in patients with HCC than those without HCC (46.25% vs. 26.67%) with a significant deviation (OR=2.36, 95%CI= 1.25- 4.49, p= 0.008). The haplotype block CCG was more common among patients with HCC (26.25%) than those without HCC (12.22%) with a significant difference (OR= 2.56, 95%= 1.14-5.71, p= 0.022). The study indicated that individuals carrying the mutant homozygous (CC) of the SNP rs8192917 and allele C of this SNP may have a higher chance of developing HCC compared with those carrying other genotypes and T allele of the SNP. The haplotype block CCG (corresponding for C allele of rs7144366, C allele of rs8192917 and G allele of rs2236338) might be regarded as a risk factor for the emergence of HCC in patients with CHB.</p>",
"DOI": "doi.org/10.5455/jabet.2022.d137",
"views": 743,
"downloads": 130,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure1.jpg",
"caption": "Figure 1. Gel electrophoresis (1% agarose) of rs7144366 gene polymorphism amplified with specific pair of primers using conventional PCR run for 45 min at 129 V. The PCR product was stained with ethidium bromide. The fragment length was 97bp.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure2.jpg",
"caption": "Figure 2. Genotyping of rs7144366 gene polymorphism after digestion with SfcI and stained with ethidium bromide. Lanes 1,3,6,7,8, and 11: TT; lanes 2,5, and 10: CT, lanes 4 and 9: CC genotype; M: DNA 50 bp DNA marker.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure3.jpg",
"caption": "Figure 3. Sequence analysis of the rs7144366, the reverse strand. The R in the upper frame represents the heterozygous genotype (TC), the G in the middle frame represents the mutant genotype (CC), and the A in the bottom frame represents the homozygous wild type of genotype (TT).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure4.jpg",
"caption": "Figure 4. Gel electrophoresis (1% agarose) of rs 8192917 gene polymorphism amplified with specific pair of primers using conventional PCR run for 45 min at 129 V. The PCR product was stained with ethidium bromide. The fragment length was 420 bp.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure5.jpg",
"caption": "Figure 5. Genotyping of rs7144366 gene polymorphism after digestion with BsmA I and stained with ethidium bromide. Lanes 1,2,4,7,8,10,11, 13 and 14: TT; lanes 5,6 and 12: CT, lanes 4 and 9: CC genotype; M: 100 bp DNA maker.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure6.jpg",
"caption": "Figure 6. Sequence analysis of the rs8192917, the reverse strand. The homozygous wild type is represented by the letter A in the top frame (TT), the G in the second line of the frame represents the mutant genotype (CC), while the R in the lower line represents the heterozygous genotype (TC).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure7.jpg",
"caption": "Figure 7. Gel electrophoresis of rs2236338 gene polymorphism amplified with specific pair of primers using conventional PCR. The PCR product was stained with ethidium bromide. The fragment length was 400 bp.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure8.jpg",
"caption": "Figure 8. Genotyping of rs2236338 gene polymorphism after digestion with BsmF I and stained with ethidium bromide. Lanes 1 and 4: GG genotype lanes 2,5,6,8,10, and 11: AA, lanes 3,7,9, and 12: AG genotype; M: 100 bp DNA marker.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure9.jpg",
"caption": "Figure 9. Sequence analysis of the rs2236338, the forward strand. The A in the upper frame represents homozygous wild type (AA), the G in the second line of the frame represent the mutant genotype (GG), while the R in lower line represents the heterozygous genotype (AG).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/58/26/178-1651419347-Figure10.jpg",
"caption": "Figure 10. Linkage disequilibrium pattern between different SNPs in GzmB gene. The red region shows strong linkage disequilibrium. D’ has a maximum value of 1.",
"featured": false
}
],
"authors": [
{
"id": 469,
"affiliation": [
{
"affiliation": "Department of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Iraq"
}
],
"first_name": "Hussein S.",
"family_name": "Alshamary",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 116
},
{
"id": 470,
"affiliation": [
{
"affiliation": "Medical Research Unit, College of Medicine, Al-Nahrain University, Iraq"
}
],
"first_name": "Qasim S. Al",
"family_name": "Mayah",
"email": "qasimsharhan@gmail.com",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Qasim S. Al-Mayah, PhD; Medical Research Unit, College of Medicine, Al-Nahrain University, Iraq, e-mail: qasimsharhan@gmail.com",
"article": 116
},
{
"id": 471,
"affiliation": [
{
"affiliation": "Department of Internal Medicine, College of Medicine, Al-Nahrain University, Iraq"
}
],
"first_name": "Fadhil Abdulla Abdul",
"family_name": "Ridha",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 116
}
]
},
{
"id": 112,
"slug": "178-1653687235-a-study-of-arginase-1-activity-and-lipid-profile-in-patients-with-myocardial-infarction",
"type": "original_article",
"manuscript_id": "178-1653687235",
"published": "2022-06-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/38/178-1653687235.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 553-561.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Anbari AAA, Alta’ee AH, et al. A study of arginase-1 activity and lipid profile in patients with myocardial infarction. J Adv Biotechnol Exp Ther. 2022; 5(3): 553-561.",
"title": "A study of arginase-1 activity and lipid profile in patients with myocardial infarction",
"abstract": "<p>Myocardial necrosis caused by ischemia is called a myocardial infarction (MI). which interrupts coronary blood supply. When the oxygen supply to the heart is insufficient to meet metabolic demands, myocardial ischemia occurs. Atherosclerosis, which obstructs the coronary arteries, is the most common underlying cause of myocardial ischemia. The role of arginase-1 (ARG-1) and serum lipids in the pathogenesis of myocardial infarction is becoming clearer. This study aims to see if there is a link between ARG-1 activity and MI in the Iraqi population. Between the first of November 2021 and the first of February 2022, 90 people were separated into two groups: 45 patients with MI and 45 healthy controls. Human ARG-1 was measured in serum blood using the ELISA method. The serum lipid was measured using the spectrophotometry technique. The current investigation discovered a substantial (p=0.01) rise in ARG-1 concentration compared to control groups, as well as a significant difference in blood lipid content between patients and control groups (p<0.05). Finally, ARG-1 may have a role to play role in the pathogenesis of MI.</p>",
"DOI": "10.5455/jabet.2022.d135",
"views": 794,
"downloads": 134,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/27/178-1653687235-Figure1.jpg",
"caption": "Figure 1. Correlation between MI patients and ARG1 activity. * Refer to groups that show significant differences when compared to control groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/27/178-1653687235-Figure2.jpg",
"caption": "Figure 2. Correlation between MI patients and lipid profile. * Refer to groups which show significant differences when compared to control groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/27/178-1653687235-Figure3.jpg",
"caption": "Figure 3. Correlation between arginase1 activity patients and lipid profile. * Refer to groups that show significant differences when compared to control groups.",
"featured": false
}
],
"authors": [
{
"id": 447,
"affiliation": [
{
"affiliation": "College of Medicine, University of Babylon, Hilla, Babylon state, 51001, Iraq"
}
],
"first_name": "Ali A. Al",
"family_name": "Anbari",
"email": "alichemicalmgcl@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ali A. Al-Anbari, PhD; College of Medicine, University of Babylon, Hilla, Babylon state, Iraq, e-mail: alichemicalmgcl@gmail.com",
"article": 112
},
{
"id": 448,
"affiliation": [
{
"affiliation": "College of Medicine, University of Babylon, Hilla, Babylon state, 51001, Iraq"
}
],
"first_name": "Abdulsamie H.",
"family_name": "Alta'ee",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 112
},
{
"id": 449,
"affiliation": [
{
"affiliation": "College of Medicine, University of Babylon, Hilla, Babylon state, 51001, Iraq"
}
],
"first_name": "Shokry F. Al",
"family_name": "Saad",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 112
}
]
},
{
"id": 115,
"slug": "178-1653047882-effect-of-kiss1-gene-variants-rs372790354-ga-and-rs4889-ga-on-kisspeptin-in-patients-with-polycystic-ovary-syndrome-in-iraq",
"type": "original_article",
"manuscript_id": "178-1653047882-",
"published": "2022-06-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/34/178-1653047882.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 562-576.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Musawi NJA, Qaysi SAA , et al. Effect of KISS1 gene variants (rs372790354 G>A and rs4889 G>A) on kisspeptin in patients with polycystic ovary syndrome in Iraq. J Adv Biotechnol Exp Ther. 2022; 5(3): 562-576.",
"title": "Effect of KISS1 gene variants (rs372790354 G>A and rs4889 G>A) on kisspeptin in patients with polycystic ovary syndrome in Iraq",
"abstract": "<p>Polycystic ovary syndrome (PCOS) is a heterogeneous genetic disorder categorized by hyperandrogenism that affects early reproductive age in females. KISS1 has played role in regulating the hypothalamic-pituitary-gonad axis. It also plays a key role in human reproductive function. Imbalance-of-function mutations is often found KISS1 gene of patients with polycystic ovary syndrome. Blood samples were collected from 120 patients (60 control are divided into 30 normal weight and 30 obese) and (60 PCOS) females are divided into 30 normal weight and 30 obese. DNA was extracted and genotyped for KISS1 variants by HRM-PCR and measured the level of kisspeptin by ELIAS, while LH, FSH, DHEA and free testosterone by CLIA. The value of LH, Testosterone, DHEA-S and kisspeptin is elevated in the patient group, while the decline of FSH in serum level patients value, rs372790354 G > A and rs4889 G>A was associated with PCOS in dominant, recessive, co-dominant (P-value< 0.05), rs37279054 AA was not found the effect of obese group and linked with normal weight PCOS put present study no effect on the parameters, rs4889 GG/GA was the effect on all subgroups except the genotype GA not effected on obese female, the highly significant ( P-value<0.05) of rs4889 GA influenced on measured of WHR, LH/FSH ratio and DHEA-S in the patient compared to control, rs4889 GG/AA was influenced on normal-weight patient compare to an obese patient, the WHR was higher in an obese patient in both genotype. While the level of kisspeptin in normal weight with genotype AA was higher level compared to obese and (P-value<0.05). We concluded that the KISS1 levels were higher in PCOS females compared to controls and decreased with increasing BMI, KISS1 polymorphism rs372790354 G>A and rs4889 G>A may be associated with the pathophysiology of PCOS and lead to increased serum level of LH that due to hyperandrogenism.</p>",
"DOI": "doi.org/10.5455/jabet.2022.d136",
"views": 1067,
"downloads": 135,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/26/178-1653047882-Figure1.jpg",
"caption": "Figure 1. Amplification and genotype of rs372790354 G>A for control and samples. A. Amplification Step, B. Genotype Step, C. Control Genotype. Yellow, GG; blue, GA; red, AA.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/57/26/178-1653047882-Figure2.jpg",
"caption": "Figure 2. Amplification and genotype of rs4889 G>A for control and samples. A. Amplification Step, B. Genotype Step, C. Control Genotype. Green, GG; blue, GA; purple, AA.",
"featured": false
}
],
"authors": [
{
"id": 459,
"affiliation": [
{
"affiliation": "DNA research center / University of Babylon, Iraq, Hillah, Babylon state, 51001, Iraq"
}
],
"first_name": "Noor J. T. Al",
"family_name": "Musawi",
"email": "noorchemist30@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Noor J. T. Al-Musawi, PhD; DNA research center / University of Babylon, Iraq, Hillah, Babylon state, 51001, Iraq, e-mail: noorchemist30@gmail.com",
"article": 115
},
{
"id": 460,
"affiliation": [
{
"affiliation": "Departments of Medical Sciences and Biochemistry, College of Medicine, University of Babylon, Babylon, Iraq"
}
],
"first_name": "Suhayr Aesa Al",
"family_name": "Qaysi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 115
},
{
"id": 461,
"affiliation": [
{
"affiliation": "Departments of obstetrics and gynecology, College of Medicine, University of Babylon, Babylon, Iraq"
}
],
"first_name": "Suha J",
"family_name": "Witwit",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 115
}
]
},
{
"id": 108,
"slug": "178-1649745934-a-hybrid-pretreatment-strategy-for-delignification-of-aloe-vera-processing-waste-and-its-effectiveness-towards-saccharification",
"type": "original_article",
"manuscript_id": "178-1649745934",
"published": "2022-06-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/05/178-1649745934.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 537-552.",
"academic_editor": "Akhi Moni, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Gunasekaran R, Jacob S. A hybrid pretreatment strategy for delignification of Aloe vera processing waste and its effectiveness towards saccharification. J Adv Biotechnol Exp Ther. 2022; 5(3): 537-552.",
"title": "A hybrid pretreatment strategy for delignification of Aloe vera processing waste and its effectiveness towards saccharification",
"abstract": "<p>A copious amount of rigid <em>Aloe vera</em> leaf rind (AVLR) has been produced from the aloe gel processing industries are majorly disposed as wastes since it has no commercial value. The cell wall compositional analysis revealed that significant quantity of cellulose (46% ± 0.76, w/w) and hemicellulose (18.5% ± 0.24, w/w) which justifies as potent source for bioethanol production. However, high lignin content (13.95% ± 0.45, w/w) hinders depolymerization of polysaccharides into fermentable sugars and subsequent fermentation for ethanol production. In the present study, microwave-assisted alkali (MAA) pretreatment of AVLR was performed by varying the power level (160 W, 320 W and 480 W) which showed a maximum delignification (66.38%) at 320 W. Scanning Electron Microscope (SEM), Fourier-transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRD) based characterization were performed to study the extent of delignification in AVLR biomass. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed for the liquid hydrolysate obtained after MAA pretreatment at 320 W indicated that the hydrolysate contained more of oxidized phenolic hydrocarbons that can be potentially utilized for other value-added product synthesis. A comparison of saccharification efficiency was performed using two different cellulase producers namely <em>Aspergillus niger</em> and <em>Aspergillus</em> sp. A 2.8-fold increase in sugar yield was achieved by <em>Aspergillus</em> sp., with a maximum saccharification of 68.5% ± 0.34 on comparing with untreated AVLR biomass. This indicates the feasibility of MAA pretreatment for AVLR biomass in order to improve the accessibility of fermentable sugars available for ethanol production.</p>",
"DOI": "10.5455/jabet.2022.d134",
"views": 1141,
"downloads": 207,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/27/178-1649745934-Figure1.jpg",
"caption": "Figure 1. SEM image representing the surface morphology of (a) Untreated AVLR, and MAA pretreatment AVLR (b) 160 W (c) 320 W (d) 480 W. (Magnification- 2,500 X under high vacuum and 20 µm).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/27/178-1649745934-Figure2.jpg",
"caption": "Figure 2. FTIR spectra of functional groups in the dried AVLR biomass obtained by KBr pelleting technique with a spectrum range of 400 cm−1 to 4000 cm−1 (a) Untreated and MAA pretreated (b) 160 W (c) 320 W (d) 480 W and X-ray diffraction patterns of (e) Untreated AVLR and MAA pretreatment AVLR (f) 160W (g) 320 W (h) 480 W.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/27/178-1649745934-Figure3.jpg",
"caption": "Figure 3. Chromatogram profile of aromatic and aliphatic hydrocarbons obtained in liquid hydrolysate after MAA pretreatment of AVLR using different polar and non-polar solvents (a) acetonitrile (b) methanol (c) hexane (d) chloroform and (e) heatmap representation of relative area proportion of intermediary degradation products.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/27/178-1649745934-Figure4.jpg",
"caption": "Figure 4. Comparison of reducing sugar yield and saccharification percentage between MAA treated and untreated AVLR after 6 h of enzymatic hydrolysis. ****p < 0.0001. The values are average of triplicate experiments and the standard deviation is represented as (±) calculated from mean and three independent trials.",
"featured": false
}
],
"authors": [
{
"id": 424,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM\r\nInstitute of Science and Technology, Kattankulathur - 603203, Chengalpattu District, Tamil Nadu, India"
}
],
"first_name": "Rajeswari",
"family_name": "Gunasekaran",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0003-1224-9762",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 108
},
{
"id": 425,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM\r\nInstitute of Science and Technology, Kattankulathur - 603203, Chengalpattu District, Tamil Nadu, India"
}
],
"first_name": "Samuel",
"family_name": "Jacob",
"email": "samueljb@srmist.edu.in",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0001-9615-153X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Samuel Jacob, PhD; Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM\r\nInstitute of Science and Technology, Kattankulathur - 603203, Chengalpattu Dist, Tamil Nadu, India, e-mail: samueljb@srmist.edu.in",
"article": 108
}
]
},
{
"id": 106,
"slug": "178-1653153639-determining-the-disease-outcome-by-cytokine-storm-during-infectious-diseases-and-targeting-cytokines-during-sepsis-possible-therapeutic-options",
"type": "review_article",
"manuscript_id": "178-1653153639",
"published": "2022-06-09",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/12/178-1653153639.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 523-536.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Amin T, Hossain A, et al. Determining the disease outcome by cytokine storm during infectious diseases and targeting cytokines during sepsis: Possible therapeutic options. J Adv Biotechnol Exp Ther. 2022; 5(3): 523-536.",
"title": "Determining the disease outcome by cytokine storm during infectious diseases and targeting cytokines during sepsis: Possible therapeutic options",
"abstract": "<p>The term ‘sepsis’ can be referred to as the dysfunction of organ(s) because of dysregulated and uncontrolled response of the host to that particular infection. As per statistics, more than 15 million sepsis cases have been recorded every year with around a 20% mortality rate. So, it is needless to mention the major threat this cytokine storm-induced syndrome is posing to public health throughout the world as both infectious and noninfectious diseases are involved with cytokine storm. A lot of evidence can be found on the major pathophysiological impact of cytokines during an infection, but no specific or effective treatment is available to target any inflammation effectively in sepsis. Numerous research has pointed out that it is possible to reduce the rate of mortality in severe sepsis by administering a low dosage of corticosteroids but unfortunately, no clinical benefits have been recorded during a large-scale clinical trial. But it is proven in a meta-analysis that anti-TNF treatment had been able to demonstrate a major reduction in the mortality rate of sepsis. The review would highlight some of the therapeutic interventions currently available to treat sepsis to get an overview. We would also focus on the association of cytokine storm in inducing sepsis.</p>",
"DOI": "10.5455/jabet.2022.d133",
"views": 1528,
"downloads": 136,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/28/27/178-1653153639-Figure1.jpg",
"caption": "Figure 1. Cytokine cascade in sepsis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/28/27/178-1653153639-Figure2.jpg",
"caption": "Figure 2. Mechanisms concerning SARS-CoV-2 induced cytokine storm.",
"featured": false
}
],
"authors": [
{
"id": 412,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Bashundhara, Dhaka, Bangladesh"
}
],
"first_name": "Tasbir",
"family_name": "Amin",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-5265-8769",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 106
},
{
"id": 413,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Bashundhara, Dhaka, Bangladesh"
}
],
"first_name": "Amana",
"family_name": "Hossain",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-1034-2173",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 106
},
{
"id": 414,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Bashundhara, Dhaka, Bangladesh"
}
],
"first_name": "Fahd Bin",
"family_name": "Zahed",
"email": "fahd.zahed@northsouth.edu",
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-2687-190X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Fahd Bin Zahed, Department of Biochemistry and Microbiology, School of Health & Life Sciences, North South University, Dhaka, Bangladesh, e-mail: fahd.zahed@northsouth.edu",
"article": 106
}
]
},
{
"id": 101,
"slug": "178-1651035415-validation-and-standardization-of-designed-n-gene-primer-based-rt-pcr-protocol-for-detecting-peste-des-petits-ruminants-virus-in-goats",
"type": "original_article",
"manuscript_id": "178-1651035415",
"published": "2022-06-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1651035415.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 497-509.",
"academic_editor": "Hasan-Al-Faruque, PhD; Daegu Gyeongbuk Institute of Sci. and Tech., South Korea",
"cite_info": "Sultana S, Pervin M, et al. Validation and standardization of designed N gene primer-based RT-PCR protocol for detecting Peste des Petits Ruminants virus in goats. J Adv Biotechnol Exp Ther. 2022; 5(3): 497-509.",
"title": "Validation and standardization of designed N gene primer-based RT-PCR protocol for detecting Peste des Petits Ruminants virus in goats",
"abstract": "<p>The reverse transcriptase-polymerase chain reaction (RT-PCR) test is one of the most popular and specific diagnostic tests to easily recognize the Peste des Petits Ruminants virus (PPRV) genome in clinical samples. The sensitivity of the RT-PCR test depends on gene-targeted primer sets. The literature appears to be lacking in designing primers used in RT-PCR to detect PPRV genome in Bangladesh. This study aimed to develop an N gene based PPRV primer set, a standardized RT-PCR protocol, and its validity test by comparison with other available primers. A total of 70 clinical samples and 10 PPRV positive isolates were used in real-time RT-PCR and conventional RT-PCR using one pair designed primer set NF/NR and three pairs of published gene F1/F2, F1b/F2b and N1/N2. N gene based PPRV primer sets (NF/NR) were designed from a published sequence of PPRV (Accession number GQ122187.1). Statistical analysis was carried out. The designed N gene-based primer positive PPRV samples were sequenced and analyzed. The N gene-based primer sets were more sensitive to PPRV detection than F gene-based primer (P =.002) in the RT-PCR test. PPRV detects the highest (86%) of clinical samples in the RT-PCR test using a designed N gene-based primer. Sequence analysis showed that designed N gene-based the 402bp sequence of PPRV isolates is clustered with other Bangladeshi PPRV isolates and belongs to Lineage IV. New primers sets were designed from the conserved region of the N gene of PPRV. Designed primer sets successfully worked in real-time RT-PCR. The standardized RT-PCR protocol with the designed primer sets (NF/NR) can be used for the specific detection of PPRV from clinical samples.</p>",
"DOI": "10.5455/jabet.2022.d131",
"views": 883,
"downloads": 151,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/27/178-1651035415-Figure1.jpg",
"caption": "Figure 1. A study layout of validation and standardization of designed N gene primer-based RT-PCR protocol for the detection of PPRV in goat.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/27/178-1651035415-Figure2.jpg",
"caption": "Figure 2. PPR virus N gene-specific designed primer NF/NR based RT-PCR amplified PPRV positive isolates as 402bp amplicons (arrow). Lanes indicate L: 100bp DNA ladder, NC: negative control, PPRV positive isolates: 1-10.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/27/178-1651035415-Figure3.jpg",
"caption": "Figure 3. Scattered dot plot for the PPRV real-time PCR assay. PPRV positive samples showed Ct value ranging from 16-25. Low viral PPRV RNA was successfully amplified in real-time PCR.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/27/178-1651035415-Figure4.jpg",
"caption": "Figure 4. PPR virus N gene-specific designed primer NF/NR based RT-PCR amplified 402bp amplicons (arrow, a). Lanes indicate L: 100bp DNA ladder, PC: positive control, NC: negative control, field samples:1-16. Other published N gene-specific primer N1/N2 based RT-PCR amplified 463bp amplicons (arrow, b). Lanes indicate L: 100bp DNA ladder, PC: positive control, NC: negative control, field samples: 1-13.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/27/178-1651035415-Figure5.jpg",
"caption": "Figure 5. PPR virus F gene specific primer F1b/F2b based RT-PCR amplified 448bp amplicons (a, arrow). Lanes indicate L: 100bp DNA ladder, NC: negative control: positive control and field samples: 1-7. Another F gene specific primer F1/F2 based RT-PCR amplified 372bp amplicons (b, arrow). Lanes indicate L: 100bp DNA ladder, NC: negative control, PC: positive control, and field samples: 1-6.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/27/178-1651035415-Figure6.jpg",
"caption": "Figure 6. Phylogenetic tree based on the partial N gene sequences of PPRV obtained from the clinical samples (detected using designed NF/NR primer) with other representative downloaded PPRV belonging to Lineages I-IV. The obtained recent field PPRV were made different cluster groups with other Bangladeshi and Asian isolates within the Lineage IV.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/27/178-1651035415-Figure7.jpg",
"caption": "Figure 7. N gene specific primers (NF/NR) were designed from a published PPRV N gene sequence GenBank Ac no. GQ122187.1 were aligned with other twelve downloaded complete N gene sequences of PPRV of India, China, Egypt, and Morocco, showed 100% homology. New NF/NR primer sets were designed from the conserved region of the N gene of PPRV.",
"featured": false
}
],
"authors": [
{
"id": 384,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sajeda",
"family_name": "Sultana",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-3902-9447",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 101
},
{
"id": 385,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Munmun",
"family_name": "Pervin",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-5514-5798",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 101
},
{
"id": 386,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Nazneen",
"family_name": "Sultana",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0001-8060-4805",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 101
},
{
"id": 387,
"affiliation": [
{
"affiliation": "Bangladesh Agricultural Research Council, Dhaka, Bangladesh."
}
],
"first_name": "Md. Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0002-8852-265X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 101
},
{
"id": 388,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mohammad Abu Hadi Noor Ali",
"family_name": "Khan",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-8852-265X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Abu Hadi Noor Ali Khan, PhD; Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University,Mymensingh-2202, Bangladesh, e-mail: hadi.khan@bau.edu.bd",
"article": 101
}
]
},
{
"id": 104,
"slug": "178-1651253654-ramipril-an-angiotensin-converting-enzyme-inhibitor-ameliorates-oxidative-stress-inflammation-and-hepatic-fibrosis-in-alloxan-induced-diabetic-rats",
"type": "original_article",
"manuscript_id": "178-1651253654",
"published": "2022-06-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/29/178-1651253654.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 510-522.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka 1230, Bangladesh",
"cite_info": "Siddiqua S, Sikder B, et al. Ramipril, an angiotensin-converting enzyme inhibitor ameliorates oxidative stress, inflammation, and hepatic fibrosis in alloxan-induced diabetic rats. J Adv Biotechnol Exp Ther. 2022; 5(3): 510-522.",
"title": "Ramipril, an angiotensin-converting enzyme inhibitor ameliorates oxidative stress, inflammation, and hepatic fibrosis in alloxan-induced diabetic rats",
"abstract": "<p>Angiotensin-II is considered as a peptide responsible for the vascular dysfunction and complications in various tissues including liver through inducing free radicle mediated oxidative stress. This study aimed to evaluate the effect of ramipril, an angiotensin-converting enzyme inhibitor (ACE inhibitor), on oxidative stress, inflammation, and fibrosis in the liver of alloxan-induced diabetic rats. In this investigation, rats were divided into four groups (six rats in each group): control, control +ramipril, alloxan, and alloxan+ ramipril. A single dose (90 mg/kg) of alloxan was given intra-peritoneally to induce type two diabetes. After the induction of diabetes, ramipril (10 mg/kg) was administered to each animal for 21 days. An oral glucose tolerance test (OGTT) was performed. All animals were sacrificed at the end of the study. Blood and liver tissues were collected from each animal and stored for further biochemical studies. Liver marker enzymes and oxidative stress parameters were also assayed followed by histological examination in the liver. Alloxan administration in rats showed oral glucose intolerance and increased fasting blood glucose levels. Ramipril (10 mg/kg) treatment in alloxan administered rats improved the OGTT and lowered fasting blood glucose level. This study also revealed the elevation of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) enzymes activities in the alloxan administered rats which were attenuated by ramipril treatment. Oxidative stress parameters such as advanced protein oxidative products (APOP), nitric oxide (NO), and malondialdehyde (MDA) were also increased in alloxan administered rats which were diminished by the treatment of ramipril. Moreover, alloxan administration increased inflammation and fibrosis in the liver, which was further prevented by ramipril treatment. In conclusion, ramipril alleviated oxidative stress and fibrosis in the liver by suppressing oxidative stress. This investigation suggests that ACE inhibitors may be useful for treating diabetic complications and liver injury in alloxan-administered rats.</p>",
"DOI": "10.5455/jabet.2022.d132",
"views": 1490,
"downloads": 149,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/27/178-1651253654-Figure1.jpg",
"caption": "Figure 1. Schematic flow chart of the experimental protocol.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/27/178-1651253654-Figure2.jpg",
"caption": "Figure 2. Effect of ramipril on oral glucose tolerance test in alloxan induced diabetes rats. Data are presented as mean±SEM, n=6. One way ANOVA with Newman-Keuls multiple comparisons test was done as post hoc test. Values are considered significance at p<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/27/178-1651253654-Figure3.jpg",
"caption": "Figure 3. Effect of ramipril treatment on hepatic inflammation in alloxan-induced rats (upper panel). A) Control, liver section showed clear hepatic tissue structure with normal bile duct and vascular area; B) Control+ ramipril, liver section showed also the clear hepatic tissue structure with normal bile duct and vascular area; C) alloxan, liver section showed necrotized hepatic tissue structure with congested and broken bile duct and vascular area followed by inflammatory mono-nuclear cells infiltration ; D) alloxan+ ramipril, liver section showed reduced necrotized zone in hepatic tissue structure with reduced inflammatory mono-nuclear cells infiltration around bile duct and vascular area. Lower panel showed the effect of ramipril treatment on hepatic fibrosis in alloxan induced rats. E) Control; liver section showed normal baseline collagen around bile duct and vascular area. F) Control+ ramipril, liver section also showed normal baseline collagen around bile duct and vascular area; G) alloxan, liver section showed increased collagen deposition around bile duct and vascular area; H) alloxan+ ramipril, liver section showed reduced collagen deposition around bile duct and vascular area. Magnification 40 X.",
"featured": false
}
],
"authors": [
{
"id": 399,
"affiliation": [
{
"affiliation": "Department of Pharmacy, East-West University, Dhaka, Bangladesh"
}
],
"first_name": "Shahnaz",
"family_name": "Siddiqua",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 104
},
{
"id": 400,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Biswajit",
"family_name": "Sikder",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 104
},
{
"id": 401,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Anayt",
"family_name": "Ulla",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 104
},
{
"id": 402,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Farzana Akhter",
"family_name": "Sumi",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 104
},
{
"id": 403,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Nasrin",
"family_name": "Akhter",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 104
},
{
"id": 404,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Md Nurul",
"family_name": "Islam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 104
},
{
"id": 405,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Md Ashraful",
"family_name": "Alam",
"email": "sonaliagun@yahoo.com",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Ashraful Alam, PhD; Department of Pharmaceutical Sciences, North-South University, Dhaka-1229, Bangladesh, e-mail: sonaliagun@yahoo.com",
"article": 104
}
]
},
{
"id": 98,
"slug": "178-1650664595-comparative-polymer-biodegradation-efficiency-of-an-isolated-acinetobacter-sp-with-bravibacillus-sp-and-e-coli-by-resting-cells",
"type": "original_article",
"manuscript_id": "178-1650664595",
"published": "2022-06-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/05/178-1650664595.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 487-496.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Akter S, Paul GK, et al. Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cells. J Adv Biotechnol Exp Ther. 2022; 5(3): 487-496.",
"title": "Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cells",
"abstract": "<p>At a concentration of 4 g/L, an enteric polymer is utilized to target drug release in the small intestine and causes considerable toxicity in cells. Our ecology and ecosystem are also harmed by their non-biodegradable qualities. We isolated and identified polymer-degrading bacteria from industrial effluent in this work. The isolated strain’s morphological, biochemical, and antibiotic sensitivities were also examined. The isolated strain was found to be gram-negative, round-shaped, and non-motile in morphological tests, while biochemical tests revealed it to be negative in starch agar and TSI but positive in methyl red, mannitol salt, simmon citrate, urea agar, and catalase test. The isolated strain was highly resistant to ciprofloxacin and vancomycin. The isolated bacterium was identified as <em>Acinetobacter</em> sp. by 16S rRNA sequencing. Additionally, <em>Acinetobacter</em> sp. strains of <em>Escherichia coli</em> and<em> Brevibacillus</em> sp. were used separately to observe the degradation of five synthesized non-biodegradable polymers (maleic acid propane-1,2 diol glycerol co-polyester, maleic acid phthalic acid propane-1,2 diol glycerol co-polyester, maleic acid phthalic acid butan-1,4 diol glycerol co-polyester, phthalic acid succinic acid propane-1,2 diol glycerol co-polyester, and phthalic acid succinic acid buten-1,4 diol glycerol co-polyester. The capacity of all three strains to degrade the above-mentioned polymers was greater than 75%. <em>E. coli</em>, for example, had a rapid disintegration rate but was responsible for human gastrointestinal and urinary tract infections. As a result, our isolated <em>Acinetobacter</em> sp. can be employed to degrade synthetic polymers.</p>",
"DOI": "10.5455/jabet.2022.d130",
"views": 945,
"downloads": 112,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/27/178-1650664595-Figure1.jpg",
"caption": "Figure 1. Phylogenetic tree analysis and agarose gel electrophoresis of 16S rRNA sequence of isolated bacterial strain. Here (a); indicate phylogenetic tree, and (b); indicate PCR amplified product on1.2% agarose gel.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/27/178-1650664595-Figure2.jpg",
"caption": "Figure 2. Antibiotic sensitivity test of isolate bacterial stain where Resistant=<10 mm; Intermediate =10-15 mm; Susceptible=>15 mm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/27/178-1650664595-Figure3.jpg",
"caption": "Figure 3. Degradation percentage of three bacterial stains by bacterial growing cells. Here (a); indicate maleic acid propen-1, 2 diol glycerol co-polyester degradation, (b); maleic acid phthalic acid propane-1, 2 diol glycerol co-polyester and (c) maleic acid phthalic acid butan-1, 4 diol glycerol co-polyester degradation. Different letters indicate significance differences between mean ± SD of replications (n=3) at a P < 0.05 significance level.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/27/178-1650664595-Figure4.jpg",
"caption": "Figure 4. Degradation percentage of three bacterial strains by bacterial growing cells. Here (a); indicate phthalic acid succinic acid propane-1, 2 diol glycerol co-polyester degradation, and (b) phthalic acid succinic acid buten-1, 4 diol glycerol co-polyester degradation. Different letters indicate significance differences between mean ± SD of replications (n=3) at a P < 0.05 significance level.",
"featured": false
}
],
"authors": [
{
"id": 371,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
},
{
"affiliation": "Major in Social Infrastructure System Science, Ibaraki University, Ibaraki 316-8511, Japan."
}
],
"first_name": "Shumona",
"family_name": "Akther",
"email": "sumona_ru10@yahoo.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "Shumona Akther, Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh, e-mail: sumona_ru10@yahoo.com",
"article": 98
},
{
"id": 372,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Gobindo Kumar",
"family_name": "Paul",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 98
},
{
"id": 373,
"affiliation": [
{
"affiliation": "Division of Genome Sciences and Cancer, The John Curtin School of Medical Research, and The Shine-Dalgarno Centre for RNA Innovation, The Australian National University, Canberra, ACT 2601, Australia."
}
],
"first_name": "Shafi",
"family_name": "Mahmud",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 98
},
{
"id": 374,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh."
}
],
"first_name": "Md. Shamim",
"family_name": "Hossain",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 98
},
{
"id": 375,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Abu",
"family_name": "Saleh",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0001-5610-4763",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 98
},
{
"id": 376,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Shahriar",
"family_name": "Zaman",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 98
},
{
"id": 377,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Salah",
"family_name": "Uddin",
"email": "salim.geb@ru.ac.bd",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Salah Uddin, Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh, e-mail: salim.geb@ru.ac.bd",
"article": 98
}
]
},
{
"id": 95,
"slug": "178-1650530315-extraction-of-squilla-harpiosquilla-annandalei-shell-derived-chitosan-and-its-nanocarrier-efficiency-for-sustained-protein-delivery",
"type": "original_article",
"manuscript_id": "178-1650530315",
"published": "2022-06-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/11/178-1650530315.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 473-486.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Balde A, Waghela B. et al. Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein delivery. J Adv Biotechnol Exp Ther. 2022; 5(3): 473-486.",
"title": "Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein delivery",
"abstract": "<p>The delivery of proteins has enormous potential in chronic disease treatment and immunomodulation. But, because of the intrinsic properties of proteins such as poor stability and quick bio clearance, their usage is limited in targeted delivery. This work suggests a cost-effective method to prepare an efficient polymer-based nanocarrier system for the sustained delivery of protein. Herein, low molecular weight chitosan (CS) was extracted from the shell waste of squilla (<em>Harpiosquilla annandalei</em>) (sCS) and characterized. It showed higher water-binding capacity (WBC) of 548 ± 11.7% as well as fat binding capacity (FBC) of 369 ± 19.9% respectively, as compared to commercial CS (cCS). BSA loaded nanoparticles were synthesized through the ionic gelation method using cCS and sCS. The <strong>s</strong>CS-based BSA encapsulated nanoparticles (BSA-sCSNPs) were observed to be uniform and small (60-195 nm) as compared to BSA loaded cCS-based nanoparticles (BSA-cCSNPs). The encapsulation efficiency (EE%) was highest for 1 mg/mL CS, BSA, and tripolyphosphate (TPP) based BSA-sCSNPs. Moreover, the <em>in vitro </em>BSA release profile exhibited that BSA-sCSNPs resulted in more stable and sustained release till the 16<sup>th </sup>h (85.4 ± 1.15%) than BSA-cCSNPs. Further, the sCS and BSA-sCSNPs were biocompatible with L929 cells and showed no cytotoxic effects. Thus, this biocompatible nanoparticle system can be used as a pharmaceutical as well as a nutraceutical agent to improve the stability as well as targeted delivery of the protein.</p>",
"DOI": "10.5455/jabet.2022.d129",
"views": 670,
"downloads": 126,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/27/178-1650530315-Figure1.jpg",
"caption": "Figure 1. Fourier transform infrared spectra of (a) cCS, (b) BSA-cCSNPs as well as (c) sCS and (d) BSA-sCSNPs, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/27/178-1650530315-Figure2.jpg",
"caption": "Figure 2. X-ray powder diffraction patterns of (a) cCS, (b) sCS and elemental analysis of (c) cCS, (d) sCS, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/27/178-1650530315-Figure3.jpg",
"caption": "Figure 3. Scanning electron micrograph of (a) cCS, (b) sCS, (c) BSA-cCSNPs and (d) BSA-sCSNPs, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/27/178-1650530315-Figure4.jpg",
"caption": "Figure 4. Particle size distribution graph of (a) BSA-cCSNPs, (b) BSA-sCSNPs and zeta potential graph of (c) BSA-cCSNPs, (d) BSA-sCSNPs, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/27/178-1650530315-Figure5.jpg",
"caption": "Figure 5. Effect of (a) Bovine serum albumin (BSA) concentration on encapsulation efficiency (EE%) of BSA encapsulated commercial chitosan nanoparticles (BSA-cCSNPs) and BSA encapsulated squilla chitosan nanoparticles (BSA-sCSNPs) where chitosan (CS) and tripolyphosphate (TPP) are 1 mg/mL. (b) CS concentration on EE% of BSA-cCSNPs and BSA-sCSNPs where TPP and BSA are 1 mg/mL. (c) TPP concentration on EE% of BSA-cCSNPs and BSA-sCSNPs where BSA and CS are 1 mg/mL, respectively and mean ± SD, n=3, *p < 0.05, **p <0.01.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/27/178-1650530315-Figure6.jpg",
"caption": "Figure 6. Effect of different chitosan (CS) and bovine serum albumin (BSA) concentrations on cumulative BSA release from (a-c) BSA encapsulated commercial chitosan nanoparticles (BSA-cCSNPs) and (b-d) BSA encapsulated squilla chitosan nanoparticles (BSA-sCSNPs) respectively where mean ± SD, n=3 and *p< 0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/27/178-1650530315-Figure7.jpg",
"caption": "Figure 7. Effect of (a) commercial chitosan (cCS), squilla chitosan (sCS) and(b) BSA encapsulated cCS nanoparticles (BSA-cCSNPs), BSA encapsulated sCS nanoparticles (BSA-sCSNPs) on the viability of L929 cells where mean ± SD, n=3 and ***p < 0.001. Untreated and Triton-X treated cells are considered as control and negative control, respectively.",
"featured": false
}
],
"authors": [
{
"id": 353,
"affiliation": [
{
"affiliation": "Biopharmaceuticals Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur603203, Chennai, Tamilnadu, India"
}
],
"first_name": "Akshad",
"family_name": "Balde",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 95
},
{
"id": 354,
"affiliation": [
{
"affiliation": "Biopharmaceuticals Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur603203, Chennai, Tamilnadu, India"
}
],
"first_name": "Bhavika",
"family_name": "Waghela",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 95
},
{
"id": 355,
"affiliation": [
{
"affiliation": "Biopharmaceuticals Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur603203, Chennai, Tamilnadu, India"
}
],
"first_name": "Nazeer Rasool",
"family_name": "Abdul",
"email": "nazeerr@srmist.edu.in",
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-0587-3770",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nazeer Rasool Abdul, PhD; Biopharmaceuticals Lab Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai 603 203, Tamilnadu, India, e-mail: nazeerr@srmist.edu.in",
"article": 95
}
]
},
{
"id": 93,
"slug": "178-1649853375-chemical-characterization-antimicrobial-antioxidant-and-larvicidal-activities-of-certain-fungal-extracts",
"type": "original_article",
"manuscript_id": "178-1649853375",
"published": "2022-05-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/27/178-1649853375.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 456-472.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka-1230, Bangladesh",
"cite_info": "Motleb AA, Aziz MA, et al. Chemical characterization, antimicrobial, antioxidant and larvicidal activities of certain fungal extracts. J Adv Biotechnol Exp Ther. 2022; 5(3): 456-472.",
"title": "Chemical characterization, antimicrobial, antioxidant and larvicidal activities of certain fungal extracts",
"abstract": "<p>Fungal extracts are considered a promising source of bioactive compounds that represent the basic core of the drug industry. This study aimed at exploring the antimicrobial, antioxidant and larvicidal activities of three ethyl acetate extracts of three fungal isolates <em>Fusarium oxysporum, Aspergillus fumigatus</em> and <em>Penicilluim griseofulvum</em>. These fungi were isolated from sediment samples collected in water courses of three Egyptian governorates; Giza, Qalubeya and Gharbeya, during a year from January to December 2019, molecularly identified using 18Sr RNA technique and were grown on rice medium for 14 days and extracted with ethyl acetate. GC-MS examination of the extracts led to identification of 15, 29 and 24 compounds, respectively. Hexadecanoic acid was detected as a main component in the investigated extracts. Moreover, in Folin-Ciocalteu’s assay, the tested fungal extracts showed noticeable total phenolic contents (TPCs) values of 115.84, 88.24 and 73.22, respectively for <em>Aspergillus fumigatus, Penicillium griseofulvum</em> and <em>Fusarium oxysporum</em>. Additionally, <em>Aspergillus fumigatus</em> extract exhibited strong total antioxidant capacity (TAC) value of 409.46 mg AAE/g dry extract, followed by <em>Penicillium griseofulvum</em> and <em>Fusarium oxysporum</em> extracts with TAC values of 299.28 and 281.31 mg AAE/g dry extract, respectively. All extracts exhibited antimicrobial activities against <em>Staphylococcus aureus</em> (G+ve bacterium), <em>Escherichia coli </em>(G-ve bacterium), <em>Candida albicans</em> (yeast) and <em>Aspergillus niger </em>(Fungus). Also, the extracts showed high larvicidal activity against miracidia and cercariae of <em>Schistosoma mansoni.</em> In conclusion, <em>Fusarium oxysporum, Aspergillus fumigatus</em> and <em>Penicilluim griseofulvum</em> are excellent sources of bioactive compounds which have multiple biological effects.</p>",
"DOI": "10.5455/jabet.2022.d128",
"views": 1370,
"downloads": 204,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/28/178-1649853375-Figure1.jpg",
"caption": "Figure 1. Phylogenetic tree of the Fusarium oxysporum f. sp. cucumerinum isolate MT1 strain. The phylogenetic tree has been reconstructed using MEGA7 software.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/28/178-1649853375-Figure2.jpg",
"caption": "Figure 2. Phylogenetic tree of the Aspergillus fumigatus isolate MT21 strain. The phylogenetic tree has been reconstructed using MEGA7 software.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/28/178-1649853375-Figure3.jpg",
"caption": "Figure 3. Phylogenetic tree of the Penicillium griseofulvum isolate MT6 strain. The phylogenetic tree has been reconstructed using MEGA7 software.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/28/178-1649853375-Figure4.jpg",
"caption": "Figure 4. GC-MS chromatogram of the ethyl acetate extract of Fusarium oxysporum f. sp. Cucumerinum.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/28/178-1649853375-Figure5.jpg",
"caption": "Figure 5. GC-MS chromatogram of the ethyl acetate extract of Aspergillus fumigatus.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/28/178-1649853375-Figure6.jpg",
"caption": "Figure 6. GC-MS chromatogram of the ethyl acetate extract of Penicillium griseofulvum.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/28/178-1649853375-Figure7.jpg",
"caption": "Figure 7. The antimicrobial activity of different isolated fungi grown on rice medium against different test microbes representing G+ve bacteria (Staphylococcus aureus), G-ve bacterium (Escherichia coli), Yeast (Candida albicans) and fungi (Aspergillus niger).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/28/178-1649853375-Figure8.jpg",
"caption": "Figure 8. Miracidial activity of (A): Fusarium oxysporum, (B): Aspergillus fumigatus and(C): Penicilluim griseofulvum fungal extracts against Schistosoma mansoni miracidia post different exposure times.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/28/178-1649853375-Figure9.jpg",
"caption": "Figure 9. Cercaricidal activity of (A): Fusarium oxysporum, (B): Aspergillus fumigatus and (C): Penicilluim griseofulvum fungal extracts against Schistosoma mansoni miracidia post different exposure times.",
"featured": false
}
],
"authors": [
{
"id": 346,
"affiliation": [
{
"affiliation": "Environmental Research and Medical Malacology Division, Theodor Bilharz Research Institute, Kornish El-Nile, Warrak El-Hadar, Imbaba, Giza 12411, Egypt"
}
],
"first_name": "Asmaa Abdel",
"family_name": "Motleb",
"email": "a_abdelmotlb@yahoo.com",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-5050-8046",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Asmaa Abdel-Motleb, Environmental Research and Medical Malacology Division, Theodor Bilharz Research Institute, Egypt, e-mail: a_abdelmotlb@yahoo.com",
"article": 93
},
{
"id": 347,
"affiliation": [
{
"affiliation": "Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornish El-Nile, Warrak El-Hadar, Imbaba, Giza 12411, Egypt"
}
],
"first_name": "Mosad A.",
"family_name": "Ghareeb",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-8398-1937",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 93
},
{
"id": 348,
"affiliation": [
{
"affiliation": "Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, Egypt"
}
],
"first_name": "Mohamed S. Abdel",
"family_name": "Aziz",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 93
},
{
"id": 349,
"affiliation": [
{
"affiliation": "Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornish El-Nile, Warrak El-Hadar, Imbaba, Giza 12411, Egypt"
}
],
"first_name": "Maha A.M. El",
"family_name": "Shazly",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 93
}
]
},
{
"id": 92,
"slug": "178-1650832128-the-correlation-between-diabetes-mellitus-and-covid-19-severity-in-babylon-province",
"type": "original_article",
"manuscript_id": "178-1650832128",
"published": "2022-05-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/02/178-1650832128.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(3): 445-455.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Hussain MA., Saadi AHA. The correlation between diabetes mellitus and COVID-19 severity in Babylon Province. J Adv Biotechnol Exp Ther. 2022; 5(3): 445-455.",
"title": "The correlation between diabetes mellitus and COVID-19 severity in Babylon Province",
"abstract": "<p>Compared to other pandemic diseases, COVID-19 had the highest transmission rate and high fatality risk. Diabetes is the hand was also one of the most frequent diseases among individuals. This study aimed to evaluate the relationship between diabetic patients infected by COVID-19 and some hematological parameters associated with diabetes and COVID-19. Patients with COVID-19 were diagnosed by PCR and/or chest computer topography (CT) scan, eight parameters were detected by AFIAS-6. The results of eight parameters for patients with diabetes mellitus infected with COVID-19 and patients with COVID-19 only showed that the Mean of Fasting Blood Glucose (FBG), glycated haemoglobin HbA1c, Insulin Sensitivity (INS) and ferritin show significant differences at (0.000, 0.000, 0.017, 0.000) respectively for the two groups, while insulin resistance (INR), insulin (IN), C-reactive protein (CRP) and D-dimer don’t show any significant differences for two groups, the statistical analysis performed at P-value ≤ 0.01 and 0.05. Infection duration results showed that the mean Insulin level (IN) and D-dimer show significant differences at (0.033 and 0.011) respectively for all infection duration categories, while FBG, HbA1c, INR, INS, CRP, and ferritin don’t show any significant differences for all day’s category. The Correlation Coefficients Between diabetes mellitus patients infected with COVID-19 and blood parameters highly correlated between FBG with INR at (0.647), HbA1c with IN at (0.078), INR with IN at (0.791), INS with CT-Scan at (0.058), CRP with D-dimer at (0.287), D-dimer with ferritin at (0.331), Ferritin with infection duration at (0.098). In conclusion, we find that the diabetes mellitus patients infected with COVID-19 suffer from a high increase of inflammatory proteins and parameters associated with diabetes compared to other patients infected with COVID-19 only, making them more susceptible to disease and more deaths compared to other people.</p>",
"DOI": "10.5455/jabet.2022.d127",
"views": 988,
"downloads": 151,
"figures": [],
"authors": [
{
"id": 344,
"affiliation": [
{
"affiliation": "Department of Biology, College of Sciences, University of Babylon, Hillah, Babylon state, 51001, Iraq"
}
],
"first_name": "Maryam A.",
"family_name": "Hussain",
"email": "maryam.ali9492@yahoo.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Maryam A. Hussain, Department of Biology, College of Sciences, University of Babylon, Hillah, Babylon state, 51001, Iraq, e-mail: maryam.ali9492@yahoo.com",
"article": 92
},
{
"id": 345,
"affiliation": [
{
"affiliation": "Department of Biology, College of Sciences, University of Babylon, Hillah, Babylon state, 51001, Iraq"
}
],
"first_name": "Ali H. Al",
"family_name": "Saadi",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-1471-2549",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 92
}
]
}
]
},
{
"id": 17,
"title": "Vol5 Issue2",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2022",
"slug": "2022-vol5-issue2",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 89,
"slug": "178-1643719256-isolation-documentation-and-biochemical-characterization-of-cellulolytic-bacteria-from-rumen-fluid-of-cattle",
"type": "original_article",
"manuscript_id": "178-1643719256",
"published": "2022-05-15",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/21/178-1643719256.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 433-444.",
"academic_editor": "Hasan-Al-Faruque, PhD; Daegu Gyeongbuk Institute of Sci. &Tech., South Korea",
"cite_info": "Poly NY, Mamtaz S, et al. Isolation, documentation, and biochemical characterization of cellulolytic bacteria from rumen fluid of cattle. J Adv Biotechnol Exp Ther. 2022; 5(2): 433-444.",
"title": "Isolation, documentation, and biochemical characterization of cellulolytic bacteria from rumen fluid of cattle",
"abstract": "<p>The microbiological use of cellulose is a significant aspect in achieving the highest possible material flow in the environment. This study aimed at isolation, identification, and biochemical characterization of bacteria with cellulase activity from cellulose samples. Cellulase enzyme has wide applications in various industries including food industry, agriculture, textile, detergent, pulp and paper, biofuel production, brewing and biorefinery. In the present study, cellulolytic bacterium <em>Pseudomonas </em>sp. was isolated from rumen fluid of cattle was collected from slaughter house. This bacterial isolate was identified by morphological, biochemical, and physiochemical characteristics. Cellulase production by the bacteria was optimized. Optimum cellulase was produced by <em>Pseudomonas</em> sp. which was observed under different pH, temperature, and incubation period. The highest production of cellulase enzyme by this bacterium was monitored at pH 7.0 for 48 hours under 3 days of cultivation at 40°C. The enzyme activity was observed at pH 7.0 with cellulosic filter paper for 5 days incubation and its maximum activity was noted at 48 hours. The result of study shows that the <em>Pseudomonas </em>sp. is a good producer of extra cellular cellulase enzyme which can be also beneficial for the degradation of cellulosic pollutants of environment.</p>",
"DOI": "10.5455/jabet.2022.d126",
"views": 1511,
"downloads": 235,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/06/178-1643719256-Figure1.jpg",
"caption": "Figure 1. Glucose standard curve for measuring glucose concentration by DNS method.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/06/178-1643719256-Figure2.jpg",
"caption": "Figure 2. Visualization of cellulolytic activity based on the cellulolytic index.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/06/178-1643719256-Figure3.jpg",
"caption": "Figure 3. Effect of different pH on cellulase production by the Pseudomonas sp.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/14/06/178-1643719256-Figure4.jpg",
"caption": "Figure 4. Effect of temperature on the production of cellulase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/14/06/178-1643719256-Figure5.jpg",
"caption": "Figure 5. Effect of incubation period on the production of cellulase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/14/06/178-1643719256-Figure6.jpg",
"caption": "Figure 6. Observation of cellulase enzyme activity on filter paper.",
"featured": false
}
],
"authors": [
{
"id": 333,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Khulna Agricultural University, Khulna -9100, Bangladesh"
}
],
"first_name": "Nilufa Yasmin",
"family_name": "Poly",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 89
},
{
"id": 334,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Sabrina",
"family_name": "Mamtaz",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 89
},
{
"id": 335,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Mohammad Mehedi Hasan",
"family_name": "Khan",
"email": "khanmmh.biochem@sau.ac.bd",
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-4814-3065",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Mehedi Hasan Khan, PhD; Department of Biochemistry and\r\nChemistry, Sylhet Agricultural University, Sylhet, Bangladesh, e-mail: khanmmh.biochem@sau.ac.bd",
"article": 89
},
{
"id": 336,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Khulna Agricultural University, Khulna -9100, Bangladesh"
}
],
"first_name": "Md. Najmol",
"family_name": "Hoque",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 89
},
{
"id": 337,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3100, Bangladesh"
}
],
"first_name": "Abul Kalam",
"family_name": "Azad",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 89
},
{
"id": 338,
"affiliation": [
{
"affiliation": "Pharmaceutical and Industrial Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Mahmudul",
"family_name": "Hasan",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 89
}
]
},
{
"id": 87,
"slug": "178-1643767322-study-on-relationship-between-genetic-abnormalities-and-clinicopathological-features-in-k-hospitals-patients-with-colorectal-cancer",
"type": "short_communication",
"manuscript_id": "178-1643767322",
"published": "2022-04-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/34/178-1643767322.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 408-416.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Vuong LD, Chu HH, et al. Study on relationship between genetic abnormalities and clinicopathological features in K hospital’s patients with colorectal cancer. J Adv Biotechnol Exp Ther. 2022; 5(2): 408-416.",
"title": "Study on relationship between genetic abnormalities and clinicopathological features in K hospital’s patients with colorectal cancer",
"abstract": "<p>The MAPK-ERK, as well as PI3K-AKT signaling transduction pathway, represents a pivotal function in tumorigenesis. Genetic alterations of potential tumor-driven genes, for instance, <em>KRAS</em>, <em>BRAF</em>, <em>NRAS,</em> and <em>PIK3CA </em>can result in uncontrolled cell proliferation and progression. The main aims of the study were not only to identify the prevalence of <em>KRAS, BRAF, NRAS, PIK3CA</em> molecular modifications but also to evaluate the relationship between gene changes and clinical and/or pathological characteristics of 251 Vietnamese colorectal cancer. Genetic abnormalities on <em>KRAS, BRAF, NRAS,</em> and <em>PIK3CA</em> were detected through the utility of Realtime PCR, Pyrosequencing, and Direct sequencing methods, respectively. The frequency of <em>KRAS, BRAF, NRAS,</em> and <em>PIK3CA</em> mutations were 34.3%, 6.4%, 7.2%, and 17.5%, in turn. <em>KRAS </em>mutation was mutually exclusive against that of <em>NRAS</em> and <em>BRAF</em> mutations in CRC. <em>BRAF</em>, as well as<em> RAS/RAF </em>mutations<em>,</em> were more usual in older age. A significant association between <em>PIK3CA</em> mutations and age together with differentiation of CRC was determined. In addition, <em>PIK3CA</em> mutation tended to coexist with <em>KRAS</em> but not with <em>NRAS</em> and <em>BRAF</em> mutation. Our results indicate the information of molecular markers that contribute to self-sufficient oncogenic mechanisms in the carcinogenesis of CRC.</p>",
"DOI": "10.5455/jabet.2022.d124",
"views": 561,
"downloads": 113,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/29/06/178-1643767322-Figure1.jpg",
"caption": "Figure 1. Representative images of H&E staining for histological subtypes. (A) Adenocarcinoma. (B) Mucinous adenocarcinoma. (C) Squamous cell carcinoma. (D) Signet ring cell carcinoma. Photographs were taken at ×200 magnification. Scale bar, 100 μm.",
"featured": false
}
],
"authors": [
{
"id": 328,
"affiliation": [
{
"affiliation": "Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam"
}
],
"first_name": "Linh Dieu",
"family_name": "Vuong",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 87
},
{
"id": 329,
"affiliation": [
{
"affiliation": "National Key Laboratory of Gene Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet\r\nStreet, Cau Giay, Hanoi, Vietnam"
}
],
"first_name": "Ha Hoang",
"family_name": "Chu",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 87
},
{
"id": 330,
"affiliation": [
{
"affiliation": "Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam"
}
],
"first_name": "Quang Ngoc",
"family_name": "Nguyen",
"email": "quangk8s@gmail.com",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Quang Ngoc Nguyen, PhD; Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam. e-mail: quangk8s@gmail.com",
"article": 87
}
]
},
{
"id": 85,
"slug": "178-1648570864-effects-of-dexamethasone-induced-stress-on-the-intestinal-morphology-and-morphometry-in-broiler-chicken",
"type": "original_article",
"manuscript_id": "178-1648570864",
"published": "2022-04-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/29/178-1648570864.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 394-407.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Islam R, Sultana N, et al. Effects of dexamethasone induced stress on the intestinal morphology and morphometry in broiler chicken. J Adv Biotechnol Exp Ther. 2022; 5(2): 394-407.",
"title": "Effects of dexamethasone induced stress on the intestinal morphology and morphometry in broiler chicken",
"abstract": "<p>The intestine of poultry plays a significant role in the health and production through enzymatic and microbial digestion of feed as well as absorption of nutrients. The current study was designed to explore the gross and histological alterations in the broiler duodenum and cecum triggered by dietary dexamethasone (DEX). The study was conducted on four homogenous groups of one-day-old chicks (20 chicks/group) i.e. one control (Non-DEX) and three treatment groups (DEX-1, DEX-2, and DEX-3). The broilers were fed commercial broiler feed containing DEX at the rate of 0, 3, 5, and 7mg/kg feed in the Non-DEX, DEX-1, DEX-2, and DEX-3 groups, respectively. The gross morphologic and morphometric data were recorded immediately after the collection of samples on days 7, 14, 21, and 28. Then, the tissue samples were processed for histological investigation. In the gross morphometric study, the weight, length, and width of the intestine were found significantly less in the DEX groups. Histopathological study results showed degeneration of intestinal glands (duodenum), mucosa, and lymphatic nodules with loss of lymphatic nodules (cecum). The percentage of the degenerated nodule was also increased. The length, width, and surface area of the duodenal villi, thickness of the mucosal layer of the cecum, and diameter of the cecal lymphatic nodules were substantially decreased in all the DEX groups. The magnitude of the alterations was associated with both the dose and duration of DEX treatment. However, the current study results indicate that DEX treatment significantly alters the morphologic and morphometric characteristics of the broiler intestine.</p>",
"DOI": "10.5455/jabet.2022.d123",
"views": 1607,
"downloads": 161,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/06/178-1648570864-Figure1.jpg",
"caption": "Figure 1. Representative images of gross view of broiler duodenum after collection of the sample on day 28.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/06/178-1648570864-Figure2.jpg",
"caption": "Figure 2. Effects of dietary DEX on the weight (gm), length (cm), and width (cm) of the duodenum in DEX treated broiler. Data were expressed as mean SEM. Columns on different days with different alphabetic superscripts are significantly (p < 0.05) different from each other.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/06/178-1648570864-Figure3.jpg",
"caption": "Figure 3. Representative photomicrographs of transverse section (H & E stained) of the duodenum from 28-day old broilers. VL- Villi length, VW- Villi width, C- Crypts, dC- Degeneration of crypts.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/06/178-1648570864-Figure4.jpg",
"caption": "Figure 4. Representative images of gross view of broiler right cecum after collection of the sample on day 28.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/06/178-1648570864-Figure5.jpg",
"caption": "Figure 5. Effects of dietary DEX on the weight (gm), length (cm), and width (cm) of the right cecum in DEX treated broiler. Data were expressed as mean SEM. Columns on different days with different alphabetic superscripts are significantly (p < 0.05) different from each other.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/06/178-1648570864-Figure6.jpg",
"caption": "Figure 5. Effects of dietary DEX on the weight (gm), length (cm), and width (cm) of the right cecum in DEX treated broiler. Data were expressed as mean SEM. Columns on different days with different alphabetic superscripts are significantly (p < 0.05) different from each other.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/06/178-1648570864-Figure7.jpg",
"caption": "Figure 7. Percentage of degenerated lymphatic nodules in cecum in the DEX treated groups on different days of the experiment.",
"featured": false
}
],
"authors": [
{
"id": 320,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh."
}
],
"first_name": "Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-6765-2693",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 85
},
{
"id": 321,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh."
}
],
"first_name": "Nasrin",
"family_name": "Sultana",
"email": "nasrin.sultana@bau.edu.bd",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0001-5498-1017",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nasrin Sultana, PhD; Associate Professor Department of Anatomy and\r\nHistology, Bangladesh Agricultural University, Mymensingh-2202,\r\nBangladesh, e-mail: nasrin.sultana@bau.edu.bd",
"article": 85
},
{
"id": 322,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh."
}
],
"first_name": "Rekha Rani",
"family_name": "Das",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 85
},
{
"id": 323,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh."
}
],
"first_name": "Sonali",
"family_name": "Bhakta",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0002-1177-068X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 85
},
{
"id": 324,
"affiliation": [
{
"affiliation": "Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh."
}
],
"first_name": "Dr.",
"family_name": "Anisuzzaman",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-7350-3093",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 85
},
{
"id": 325,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh."
}
],
"first_name": "Ziaul",
"family_name": "Haque",
"email": null,
"author_order": 6,
"ORCID": "http://orcid.org/0000-0002-8000-4575",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 85
},
{
"id": 326,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh."
}
],
"first_name": "Mohammad Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 85
}
]
},
{
"id": 84,
"slug": "178-1647620861-evaluation-of-antibacterial-antibiofilm-activity-of-biosynthesis-mgonps-and-cellular-immunity-in-rabbit",
"type": "original_article",
"manuscript_id": "178-1647620861",
"published": "2022-04-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/02/178-1647620861.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 381-393.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh.",
"cite_info": "Hassan D, Abd F, et al. Evaluation of antibacterial, antibiofilm activity of biosynthesis MgONPs and cellular immunity in rabbit. J Adv Biotechnol Exp Ther. 2022; 5(2): 381-393.",
"title": "Evaluation of antibacterial, antibiofilm activity of biosynthesis MgONPs and cellular immunity in rabbit",
"abstract": "<p>Magnesium oxide (MgO) is one of the most promising nanoparticles due to its mono-metallic oxide group, High melting point, no toxicity issues, high hardness, and high purity. For those reasons, these materials were used in many fields including medicine, agriculture, electronics, energy, and environmental protection. <em>Leuconostoc</em> spp. was used as a facility to biosynthesize MgO nanoparticles, then optimized pH and Mg (NO3)2.6H2O concentration for its antibacterial activity and cellular immunity reactions including skin sensitivity through studying Interleukin 1-Beta (IL-1β), Interleukin 17 (IL-17) and Interleukin 2 (IL-2) responding. The study shows that the optimum pH for Magnesium Oxide (MgO) biosynthesis was 12, optimum Mg (NO3)2.6H2O concentration was 0.1 M, while antibacterial activity shows a high effect on Gram-negative bacteria with 125 µg/ml as minimum inhibiting concentration (MIC) and 500 µg/ml as minimum bactericidal concentration (MBC), the immunity study appeared that the MgO nanoparticles (MgONPs) altered the immunity response in skin test duration and diameter of MgONPs as 1.31±0.55, 9.33±1.15, 11.33±1.75 and 8.33±0.75 mm after 4,24,84 and 72 hours, respectively. IL-1β, IL-17 and IL-2 show significant differences compared to the control group at P-value ≤ 0.05 as 82.305±13.38, 101.444±16.943 and 49.781±5.264, respectively. MgONPs can be used as an alternative treatment for multidrug resistance MDR bacteria due to their high effectiveness against bacterial growth, MgONPs induced the cellular immunity response in rabbit tissues as delivery of immunogen.</p>",
"DOI": "10.5455/jabet.2022.d122",
"views": 628,
"downloads": 92,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/04/07/178-1647620861-Figure1.jpg",
"caption": "Figure 1. Different pH values to detect the best pH for Leuconostoc spp. for MgONPs synthesis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/04/07/178-1647620861-Figure2.jpg",
"caption": "Figure 2. The production of MgONPs that synthesis by isolating Leuconostoc spp. in different concentrations of salt.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/04/07/178-1647620861-Figure3.jpg",
"caption": "Figure 3. Effect of MgONPs with Antibiotics by agar well diffusion test against Salmonella typhi. A: Antibiotics CIP and MgONPs with Antibiotics CIP B: Antibiotics GN and MgONPs with Antibiotics GN, C: MgONPs only.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/04/07/178-1647620861-Figure4.jpg",
"caption": "Figure 4. Skin test of the immunized rabbits (A) as control after 72 hrs, and (B) represented as immunized rabbits with MgONPs after 24 hrs.",
"featured": false
}
],
"authors": [
{
"id": 317,
"affiliation": [
{
"affiliation": "Al-Furat Al-Awsat Technical University, Babylon Institute, Medicine Laboratory, Hillah, Iraq"
}
],
"first_name": "Duaa",
"family_name": "Hassan",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-1240-9829",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 84
},
{
"id": 318,
"affiliation": [
{
"affiliation": "Biology Department, College of Sciences, University of Babylon, Babylon, Hillah, Iraq"
}
],
"first_name": "Frial",
"family_name": "Abd",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-5188-0656",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 84
},
{
"id": 319,
"affiliation": [
{
"affiliation": "DNA Research Center, University of Babylon, Babylon, Hillah, Iraq"
}
],
"first_name": "Lubna",
"family_name": "Albayati",
"email": "albayatilubna@yahoo.com",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Lubna Albayati, PhD; DNA Research Center, University of Babylon, Babylon, Hillah, Iraq, e-mail: albayatilubna@yahoo.com",
"article": 84
}
]
},
{
"id": 79,
"slug": "178-1647728954-micro-rna-evaluation-specification-and-stabilization-study-in-mixednon-mixed-body-fluids-as-a-specific-molecular-marker",
"type": "original_article",
"manuscript_id": "178-1647728954",
"published": "2022-04-17",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/31/178-1647728954.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 347-357.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Al-Mawlah YH, Naji MZ, et al. Micro-RNA evaluation, specification, and stabilization study in mixed/non-mixed body fluids as a specific molecular marker. J Adv Biotechnol Exp Ther. 2022; 5(2): 347-357.",
"title": "Micro-RNA evaluation, specification, and stabilization study in mixed/non-mixed body fluids as a specific molecular marker",
"abstract": "<p>Body fluids are frequently recovered by forensic investigators from crime scenes, and their identification is an important aspect of forensic case analysis. Determining the type and origin of human fluids recovered at a crime scene will give essential information for crime scene reconstruction by establishing a link between sample donors and actual criminal activity. The expression levels of microRNAs (miRNAs) such as miR-10b, miR-135b, miR-16, miR-126, miR-124-3p, and miR-372 in seminal fluids, blood, vaginal fluid stains, and their mixture, were measured using a quantitative real-time PCR technique. Using SNORD-47 as a reference gene, the target genes’ identification and stability were assessed. miR-10b and miR-135b were expressed at higher levels in seminal fluids stains than in vaginal secretion stains; miR-124-3p and miR-372 were expressed at higher levels in vaginal stains than in seminal fluids stains; miR-16 and miR-126 were expressed at a higher level in bloodstains than in both seminal fluids and vaginal stains, and six miRNAs were expressed in seminal fluids/vaginal/blood mix stains. In conclusion, the results indicate that the expression levels of miR-10b and miR-135b (seminal fluids), miR-124-3p and miR-372 (vaginal secretion), and miR-16 and miR-126 (blood) were higher in these samples compared to the housekeeping gene SNORD-47.</p>",
"DOI": "10.5455/jabet.2022.d120",
"views": 190,
"downloads": 66,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/47/07/178-1647728954-Figure1.jpg",
"caption": "Figure 1. Gene expression values of mixed sample (seminal fluid/vaginal secretion). A) Untreated fresh sample. B) Treated sample (two weeks at room temperature and dark).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/47/07/178-1647728954-Figure2.jpg",
"caption": "Figure 2. Gene expression values of mixed sample (blood/seminal fluid). A) Untreated fresh sample. B) Treated sample (two weeks at room temperature and dark).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/47/07/178-1647728954-Figure3.jpg",
"caption": "Figure 3. Gene expression values of mixed sample (blood/vaginal secretion). A) Untreated fresh sample. B) Treated sample (two weeks at room temperature and dark).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/47/07/178-1647728954-Figure4.jpg",
"caption": "Figure 4. miRNAs gene expression fold differed significancy between fresh and treated mixed samples, A) Seminal fluids and vaginal secretions (Sig. of miR-10b: 0.089, miR135b: 0.090, miR-124-3p: 0.009 and miR-372: 0.008, P value ≤ 0.05). B) Blood and seminal fluids (Sig. of miR-16:0.526, miR-126:0.954, miR-10b: 0.323, miR135b: 0.110, P value ≤ 0.05). C) Blood and vaginal secretion (Sig. of miR-16:0.82, miR-126:0.954, miR-124-3p: 0.21, miR-372: 0.198, P value ≤ 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/47/07/178-1647728954-Figure5.jpg",
"caption": "Figure 5. Summary of the study. It shows the fluid that might be found in a crime scene especially when it's found mixed with one or more another type of fluid, and the role of micro-RNA molecules to identify each type of fluid considering that the micro-RNA molecules are tissue-specific molecules. The figure is created with BioRender.com.",
"featured": false
}
],
"authors": [
{
"id": 297,
"affiliation": [
{
"affiliation": "DNA Research Center, University of Babylon, Babylon, Hillah-Najaf Street, 51001, Iraq"
}
],
"first_name": "Yasir Haider",
"family_name": "Al-Mawlah",
"email": "yasser.almawla@uobabylon.edu.iq",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-2453-9567",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Yasir Haider Al-Mawlah, PhD; DNA Research Center, University of Babylon, Babylon, Hillah-Najaf Street, 51001, Iraq, e-mail: yasser.almawla@uobabylon.edu.iq",
"article": 79
},
{
"id": 298,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Techniques, Al-Mustaqbal University College, Hillah-Najaf Street, 51001, Iraq"
}
],
"first_name": "Mohammed Zuhair",
"family_name": "Naji",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 79
},
{
"id": 299,
"affiliation": [
{
"affiliation": "Department of Medical Laboratory Techniques, Al-Mustaqbal University College, Hillah-Najaf Street, 51001, Iraq"
}
],
"first_name": "Mustafa Jawad",
"family_name": "Al-Imari",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-7313-7580",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 79
},
{
"id": 300,
"affiliation": [
{
"affiliation": "Continuous Education Department, University of Al-Ameed, Karbala, 56001, Iraq"
}
],
"first_name": "Hadi Sajid",
"family_name": "Abdulabbas",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0002-4336-9022",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 79
}
]
},
{
"id": 81,
"slug": "178-1646022244-htra1-and-htra2-expression-differentially-modulate-the-clinical-prognosis-of-cancer-a-multi-omics-analysis-using-bioinformatics-approaches",
"type": "original_article",
"manuscript_id": "178-1646022244",
"published": "2022-04-17",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/15/178-1646022244.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 358-380.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Reza A, Tarik MTE, et al. HTRA1 and HTRA2 expression differentially modulate the clinical prognosis of cancer: a multi-omics analysis using bioinformatics approaches. J Adv Biotechnol Exp Ther. 2022; 5(2): 358-380.",
"title": "HTRA1 and HTRA2 expression differentially modulate the clinical prognosis of cancer: a multi-omics analysis using bioinformatics approaches",
"abstract": "<p>Globally the most common cause of human death is cancer. But still, all information is unavailable to detect cancer in the early stage, and the relation of groups of the gene with cancer has to explore. <em>HTRA</em> serine proteases facilitate cellular homeostasis. Oncogenesis and response to treatment have been connected to their dysfunction in several typical human tissues. The present study aimed to assess the expression pattern of <em>HTRA1</em> and <em>HTRA2</em> in different cancers and evaluate the prognostic outcome in cancer. Various bioinformatics databases and methodologies are used in this study, including Oncomine, Kaplan-Meier plotter, OncoLnc, R2 platform, PrognoScan databases, GEPIA, cBioPortal, STRING, KEGG, and Reactome pathways analyses. The expression of <em>HTRA1</em> and <em>HTRA2</em> was analyzed, and different web-based bioinformatics platforms were used to determine their functional protein partners and correlated genes. Moreover, the cross-cancer interaction between <em>HTRA1</em> and <em>HTRA2</em> with mutations and CNAs was explored. GO and pathway analysis was used to assess the impact of these associated characteristics on certain cancers. In prognosis analysis, a positive correlation was found between <em>HTRA1</em> overexpression and poor prognosis in pancreatic, kidney, colon, and rectum cancer. A significant positive relationship between <em>HTRA2</em> overexpression and poor survival was found in pancreatic, skin, and colon cancer patients. We found that <em>HTRA1</em> in individuals with pancreatic, kidney, and colon cancers might be targeted for cancer therapy, and <em>HTRA2</em> could be used as a prognostic biomarker for skin, pancreatic, and colon cancers. Meantime, both genes might be possible targets for colon and pancreatic cancer.</p>",
"DOI": "10.5455/jabet.2022.d121",
"views": 1323,
"downloads": 129,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/11/07/178-1646022244-Figure1.jpg",
"caption": "Figure 1. Transcription rates of HTRA1 and HTRA2 in various cancer types. (a) The above figure was obtained from the Oncomine database, showing statistically significant data of HTRA1 and HTRA2 mRNA with upregulation (indicated by red) and downregulation (indicated by blue) (cancer tissue vs. normal tissue). We used 0.01, 2, and 10% for p-value, fold change, and gene ranking, respectively, as our criteria for analysis. The number of studies that met these criteria is represented by the numbers in the boxes. (b) Study of mRNA levels for HTRA1 and HTRA2 in 32 forms of cancer utilizing cBioPortal online tool. Each point reflects an individual analysis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/11/07/178-1646022244-Figure2.jpg",
"caption": "Figure 2. Analysis of HTRA1 mRNA expression in different human cancers. (a) The HTRA1 expression box plot was constructed from the Oncomine online tool. The left plot represents the normal tissue, while the cancer tissue is seen in the right plot. Analysis of MG (Malignant Glioma) compared with normal cerebellum (i), DLBC (Lymphoid Neoplasm Diffuse Large B-cell Lymphoma) compared with normal B-lymphocyte (ii), OV (Ovarian cancer) compared with normal ovarian surface epithelium (iii), PDAC (Pancreatic Ductal Adenocarcinoma) compared with the normal pancreas (iv), KIRC (Clear Cell Renal Cell Carcinoma compared with normal kidney cell (v), SKCM (Cutaneous Melanoma compared with normal skin (vi), COAD (Colon adenocarcinoma) compared with a normal colon (vii), READ (Rectum adenocarcinoma) compared with the normal rectum. (b) Analysis of HTRA1 expression in The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTex) database. The box plot was generated using GEPIA to show the expression patterns of HTRA1 in tumor (T) tissues compared to its normal (N) tissue. The left red plot represents tumor tissue, while the right blue blot reflects normal tissue (i-viii). The criteria of this analysis were 0.01 and 2 for p-value and fold change, respectively. GBM (Glioblastoma multiforme), DLBC (Lymphoid Neoplasm Diffuse Large B-cell Lymphoma), OV (Ovarian carcinoma), PAAD (Pancreatic adenocarcinoma), KIRC (Kidney renal clear cell carcinoma), SKCM (Skin Cutaneous Melanoma), COAD (Colon adenocarcinoma), READ (Rectum adenocarcinoma).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/11/07/178-1646022244-Figure3.jpg",
"caption": "Figure 3. Analysis of HTRA2 mRNA expression in different human cancers. (a) The HTRA2 expression box plot was constructed from the Oncomine online tool. The left plot represents the normal tissue, while the cancer tissue is seen in the right plot. Analysis of GBM (Glioblastoma) compared with the cerebellum (i), DLBC (Lymphoid Neoplasm Diffuse Large B-cell Lymphoma) compared with B-lymphocyte (ii), OV (Ovarian cancer) compared with ovarian surface epithelium (iii), PDAC (Pancreatic Ductal Adenocarcinoma) compared with the pancreas (iv), ccRCC (Clear Cell Renal Cell Carcinoma) compared with kidney (v), SKCM (Skin Cutaneous Melanoma) compared with skin (vi), COAD (Colon adenocarcinoma) compared with a normal colon (vii), BRCA (Invasive breast carcinoma) compared with breast (viii). (b) Analysis of HTRA2 expression in The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTex) database. The box plot was generated using GEPIA to show the expression patterns of HTRA2 in tumor (T) tissues compared to its normal (N) tissue. The left red plot represents tumor tissue, while the right blue blot reflects normal tissue (i-viii). The criteria of this analysis were 0.01 and 2 for p-value and fold change, respectively. GBM (Glioblastoma multiforme), DLBC (Lymphoid Neoplasm Diffuse Large B-cell Lymphoma), OV (Ovarian carcinoma), PAAD (Pancreatic adenocarcinoma), KIRC (Kidney renal clear cell carcinoma), SKCM (Skin Cutaneous Melanoma), COAD (Colon adenocarcinoma), BRCA (Invasive breast carcinoma).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/11/07/178-1646022244-Figure4.jpg",
"caption": "Figure 4. Analysis of promoter methylation in both HTRA1 and HTRA2 genes. The box plots were obtained from UALCAN online database. (a) The figure shows the methylation status of the HTRA1 promoter in several human cancers (i-vii). (b) The figure shows the methylation status of HTRA2 promoter in several human cancers (i-vii). BLCA, urothelial bladder carcinoma; BRCA, breast invasive carcinoma; COAD, colon adenocarcinoma; ESCA, esophageal carcinoma; HNSC, head, and neck squamous cell carcinoma; KIRC, kidney renal clear cell carcinoma; LIHC, liver hepatocellular carcinoma; LUAD, lung adenocarcinoma; PRAD, prostate adenocarcinoma.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/11/07/178-1646022244-Figure5.jpg",
"caption": "Figure 5. Recognition of protein-protein interactions necessary for HTRA1 and HTRA2 function. (a) The connecting nodes obtained using STRING are shown in circles. The predicted protein partners of HTRA1 and HTRA2 are shown in figures (i) and (ii), respectively. (b) This figure shows a total of 219 mutations, including 192 missense mutations, 19 truncating mutations, four in-frame mutations, and four fusion mutations. All the mutation sites are localized from 0 to 480 amino acids residue (i). A total of 170 mutations, including 131 missense mutations, 37 truncating mutations, and two in-frame mutations, are located between amino acids 0 and 458 (ii). The cancer types summary indicates that the HTRA1 and HTRA2 genes have predominantly altered in skin cancer (melanoma for HTRA1 and metastatic melanoma for HTRA2).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/11/07/178-1646022244-Figure6.jpg",
"caption": "Figure 6. (a) Frequency of genetic changes in HTRA1-centered-signature-gene (HTRA1, RPL34, ACAN, YME1L1, HSPD1, CLPP, PLEKHG4, CFH, ARMS2, CLPX, CTRC) was retrieved from cBioPortal database, which includes a minimum of 20% alterations. The color of the bar indicates the alteration types. Green color indicates mutation, purple indicates fusion, red indicates amplification, blue indicates deep deletion, and grey indicates multiple alterations in the alteration frequency (i). The alteration frequency of HTRA2-centered-signature-gene (HTRA2, BIRC2, BIRC3, ENDOG, PINK1, PARK2, TIA1, MAPK14, TRAF2, DIABLO, XIAP) was retrieved from cBioPortal database which includes a minimum of 15% alterations. The color of the bar indicates the alteration types. Green color indicates mutation, purple indicates fusion, red indicates amplification, blue indicates deep deletion, and grey indicates multiple alterations in the alteration frequency (ii). (b) The HTRA1-centered-gene-signature alterations most commonly occur in cutaneous squamous cell carcinoma (SCC). We explored the OncoPrint feature to identify how genomic alterations are distributed through the HTRA1 and its protein partners gene within cutaneous squamous cell carcinoma. A missense mutation (green), splice fusion (yellow), truncating mutation (purple), and no alterations (grey) were included in the alteration frequency (i). The HTRA2-centered-gene-signature alterations most commonly occur in gastric cancer. We explored the OncoPrint feature in order to identify how genomic alterations are distributed through the HTRA1 and its protein partners gene within gastric cancer. A missense mutation (green), splice mutation (yellow), deep deletion (blue), amplification (red), and no alterations (grey) were included in the alteration frequency (ii).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/11/07/178-1646022244-Figure7.jpg",
"caption": "Figure 7. HTRA1 and HTRA2 gene expression with their clinical significance. (a) Survival plot generated from PrognoScan with HTRA1 expression pattern (red, high expression; blue, low expression) for (i) brain cancer, (ii) blood cancer. Survival plot generated from Kaplan-Meier Plotter with HTRA1 expression pattern (red, high expression; black, low expression) for (iii) ovarian cancer. Survival plot generated from R2: Kaplan Meier scanner with HTRA1 expression pattern (red, high expression; blue, low expression) for (vi) Pancreatic cancer, (v) kidney cancer, (vii) skin cancer, (vii) colon cancer. Survival plot generated from OncoLnc with HTRA1 expression pattern (red, high expression; blue, low expression) for (ix) rectum cancer. The analysis threshold was Cox p <0.05 for all databases. (b) Survival plot generated from R2: Kaplan Meier scanner with HTRA2 expression pattern (red, high expression; blue, low expression) for (i) brain cancer, (ii) blood cancer, (iii) pancreatic cancer, (v) kidney cancer, (vii) colon cancer. Survival plot generated from Kaplan-Meier plotter with HTRA2 expression pattern (red, high expression; black, low expression) for (iii) ovarian cancer. Survival plot generated from PrognoScan with HTRA2 expression pattern (red, high expression; blue, low expression) for (vi) skin cancer, (viii) breast cancer. The analysis threshold was Cox p <0.05 for all databases.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/11/07/178-1646022244-Figure8.jpg",
"caption": "Figure 8. Study of the correlated gene in selected four types of cancer. These correlated genes were obtained from the R2 database. Venny 2.1 was used to create the Venn diagram for identifying the common genes. (a) The Venn diagram shows the genes positively correlated with HTRA1 in leukemia, lymphoma, breast, and pancreatic cancer (i). The Venn diagram shows the genes positively associated with HTRA2 in lymphoma, skin, kidney, and pancreatic cancer (ii). (b) Venn diagram of the negatively correlated gene with HTRA1 (i) and HTRA2 (ii) in selected four cancers.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/11/07/178-1646022244-Figure9.jpg",
"caption": "Figure 9. Pathways and gene ontologies related to HTRA1, HTRA2, and correlated genes. Pathways are obtained from Enrichr online database. The color gradient and the bar length reflect the significance level of the study (brighter color represents more significance). (a) GO and pathways of HTRA1. (i) KEGG human 2019, (ii) Reactome 2016, (iii) Panther 2016, (iv) GO biological process 2018, (v) GO molecular function 2018, and (vi) GO cellular component 2018. (b) GO and pathways of HTRA2. (i) KEGG human 2019, (ii) Reactome 2016, (iii) Panther 2016, (iv) GO biological process 2018, (v) GO molecular function 2018, and (vi) GO cellular component 2018.",
"featured": false
}
],
"authors": [
{
"id": 301,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Abu",
"family_name": "Reza",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-5934-4850",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 81
},
{
"id": 302,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Md. Tasnim-E",
"family_name": "Tarik",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 81
},
{
"id": 303,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Rizone Al",
"family_name": "Hasib",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0003-3300-6751",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 81
},
{
"id": 304,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Md. Khasrul",
"family_name": "Alam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 81
},
{
"id": 305,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Bangladesh."
}
],
"first_name": "Md. Shahedur",
"family_name": "Rahman",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 81
},
{
"id": 306,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Mohammad Abu Hena Mostofa",
"family_name": "Jamal",
"email": "jamalbtg@gmail.com",
"author_order": 6,
"ORCID": "http://orcid.org/0000-0003-0542-4323",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Abu Hena Mostofa Jamal, Department of Biotechnology and\r\nGenetic Engineering, Faculty of Biological Sciences, Islamic University,\r\nKushtia-7003, Bangladesh, email: jamalbtg@gmail.com",
"article": 81
}
]
},
{
"id": 78,
"slug": "178-1642570027-optimization-of-surface-sterilization-method-for-the-isolation-of-endophytic-fungi-associated-with-curcuma-longa-l-and-their-antibacterial-activity",
"type": "original_article",
"manuscript_id": "178-1642570027",
"published": "2022-04-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/07/178-1642570027.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 334-346.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh.",
"cite_info": "Deepak H, Virk V. Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity. J Adv Biotechnol Exp Ther. 2022; 5(2): 334-346.",
"title": "Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity",
"abstract": "<p>Medicinal plants have been extensively studied since ancient times and exploited for their various therapeutic applications. These plants have a repository of numerous beneficial chemical constituents imputable to their possible bioactive metabolites. Throughout the ages, turmeric (<em>Curcuma longa</em> L.) has a renowned nutritional and therapeutic importance. Each part of turmeric like rhizome, roots, stems, and leaves has its own medicinal properties. Inorder to recover endophytic mycoflora, epiphytic mycoflora must be eliminated via different sterilization techniques. In the present study, different sterilization methods have been assessed to isolate fungal endophytes from different parts of <em>Curcuma longa</em> L. The method involving the sequential washing of plant tissues with ethanol, sodium hypochlorite and ethanol used found more proficient in eliminating epiphytes. Five different surface sterilization treatments were evaluated for rhizome, roots, stem, and leaves of turmeric plant. Moreover, treatment 5 with combination of 70% ethanol for 1 min., followed by 2% sodium chloride for 2 to 3 min (2 min for leaf, and stem; 3 min for roots and rhizome) and 70 % ethanol for 30 seconds was found effective. A total of 38 fungal endophytes were recovered and screened for antibacterial activity against human pathogens <em>Staphylococcus aureus</em>, <em>Bacillus cereus</em>, <em>Pseudomonas aeruginosa</em> and <em>Escherichia coli</em>. Out of 38 isolates, only 11 (28.9%) isolates were found effective in inhibiting either one or all test pathogens. The results confirmed that turmeric plant of Haridwar region represents an extremely rich reservoir of potential endophytic mycoflora with possible source of novel bioactive metabolites. Further studies will resolute the potential of endophytic fungi for various biological activities such as antioxidant, antidiabetic and immunomodulatory.</p>",
"DOI": "10.5455/jabet.2022.d119",
"views": 2758,
"downloads": 459,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1642570027-Figure1.jpg",
"caption": "Figure 1. Collection site of Curcuma longa L. (Turmeric).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1642570027-Figure2.jpg",
"caption": "Figure 2. Contamination and survival percentage of isolates the surface sterilization treatment.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1642570027-Figure3.jpg",
"caption": "Figure 3. Efficiency of surface sterilization method on potato dextrose agar.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1642570027-Figure4.jpg",
"caption": "Figure 4. Recovery percentage of endophytic fungi on different growth media.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1642570027-Figure5.jpg",
"caption": "Figure 5: Colony morphology of endophytic fungal isolates on different media A) RBA, B) PDA, and C) WA.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1642570027-Figure6.jpg",
"caption": "Figure 6. Antibacterial activity of isolated fungal endophytes on MHA (Muller Hinton Agar Medium) against (A) E. coli, (B) S. aureus, (C) P. aeruginosa and (D) B. cereus.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1642570027-Figure7.jpg",
"caption": "Figure 7. The isolation pattern for endophytic fungi. Firstly, the whole plant washed with running tap water for 10-15 minutes in order to remove the dirt and debris attached to the surface. After washing, the plant parts were subjected to surface sterilization treatment, 70 % ethanol, 2-2.5 % sodium hypochlorite and 70 % ethanol. After surface sterilization, tissue fragments were placed onto the desired growth medium and incubated at 25±2°C for 7 to 15 days. After incubation, the growing hyphal regions were aseptically transferred to PDA to get pure colonies.",
"featured": false
}
],
"authors": [
{
"id": 286,
"affiliation": [
{
"affiliation": "Department of Microbiology, Kanya Gurukula Campus, Gurukula Kangari (deemed to be University), Haridwar-249407, India."
}
],
"first_name": "Himani",
"family_name": "Deepak",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 78
},
{
"id": 287,
"affiliation": [
{
"affiliation": "Department of Microbiology, Kanya Gurukula Campus, Gurukula Kangari (deemed to be University), Haridwar-249407, India."
}
],
"first_name": "Verinder",
"family_name": "Virk",
"email": "virender.wahla@gkv.ac.in",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Verinder Virk, PhD ; Department of Microbiology, Kanya Gurukula Campus, Gurukula Kangari (deemed to be University), Haridwar-249407, India, email: virender.wahla@gkv.ac.in",
"article": 78
}
]
},
{
"id": 65,
"slug": "178-1646159464-quantitative-analysis-of-the-factors-influencing-ida-and-tsh-downregulation-in-correlation-to-the-fluctuation-of-activated-vitamin-d3-in-women",
"type": "original_article",
"manuscript_id": "178-1646159464",
"published": "2022-04-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/17/178-1646159464.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 320-333.",
"academic_editor": "Md Jamal Uddin, PhD, ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Azad SA, Ahmed S, et al. Quantitative analysis of the factors influencing IDA and TSH downregulation in correlation to the fluctuation of activated vitamin D3 in women. J Adv Biotechnol Exp Ther. 2022; 5(2): 320-333.",
"title": "Quantitative analysis of the factors influencing IDA and TSH downregulation in correlation to the fluctuation of activated vitamin D3 in women",
"abstract": "<p>Anemia and thyroid disorders are global health issues that affect all ages but are more apparent in women. In this case, some serological components responsible for iron deficiency anemia (IDA) and thyroid-stimulating hormone (TSH) downregulation in women have been found actively regulated through a complex vitamin D3 mediated mechanism. This research has been investigated the correlation between activated vitamin D3 and the serological components responsible for IDA and dysregulation of TSH in childbearing and non-child-bearing women of different health conditions. Experimental sampling from 482 women suffering from both IDA and TSH dysregulation was taken, aged between 0 and 70 years. Serological parameters, such as iron, total iron-binding capacity, and ferritin, were assessed for IDA profiling, whereas thyroid-stimulating hormone and free thyroxin were for TSH profiling based on the individual’s serum vitamin D3 concentration. The resulting serological data were interpreted using sophisticated computer programming language and algorithms for quantitative biochemical analysis. The study resulted in a significant correlation between FT4 and vitamin D3 (p < 0.0001) for all age groups. TSH also showed strong interactions with the fluctuation of vitamin D3 levels (p < 0.0001), except for the children aged below 10 years (p < 0.063). The iron, TIBC, TSH, and FT4 showed phenomenal regulation with the steroidal-vitamin D3 concentration for congenital patients. Unlike the others, ferritin has a substantial connection with activated Vitamin D3 (p < 0.0064) fluctuation in the serum. To ratify, the concentrations of TSH, FT4, iron, TIBC, and ferritin were found to be significantly interconnected in terms of serum vitamin D3 concentration in women suffering from IDA and TSH downregulation simultaneously. In addition, the BMI condition of the patients can be a major factor in terms of correlating vitamin D3 with the regulatory factors of IDA and thyroid TSH as resulted in this research. To understand the accuracy and efficacy of the serum vitamin D3 in IDA and TSH downregulation, some other inflammatory markers and parathyroid hormone analysis of many samples can be conducted in continuation of this study.</p>",
"DOI": "10.5455/jabet.2022.d118",
"views": 1513,
"downloads": 122,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/07/178-1646159464-Figure1.jpg",
"caption": "Figure 1. (A) The correlation between the thyroid-stimulating hormone (TSH) and vitamin D3; (B) The correlation between free thyroxin (FT4) and vitamin D3 status in thyroid dysregulated women patients. µIU/mL (micrograms international units per milliliter), ng/mL (nanograms per milliliter), LS mean (the mean of Longitudinal Section), SE (standard error), CI (confidence interval).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/07/178-1646159464-Figure2.jpg",
"caption": "Figure 2. (A) The correlation between iron and vitamin D3; (B) The correlation between TIBC and vitamin D3; and (C) The correlation between ferritin and vitamin D3 status in anemic women of reproductive ages. TIBC (total iron-binding capacity), µg/dL (micrograms per deciliter), ng/mL (nanograms per milliliter).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/07/178-1646159464-Figure3.jpg",
"caption": "Figure 3. (A) Two-way ANOVA and ‘Tukey’s multiple t-tests’ are showing the correlation of the variables with vitamin D3 among 13 congenital cases of IDA and TSH disorder. The stacked columns demonstrate the relationship of Iron, TIBC, Ferritin, TSH, and FT4 against vitamin D3 in the case of 13 congenital patients who had iron deficiency anemia and thyroid deficiency. (B) The level of divergence of the obtained values (from Figure 3A) contains 95% confidence considering the DBPM, SED, and CID parameters. The level of significance of correlation of vitiation D3- with the IDA causing parameters are mentioned as (*), while parameters regulating thyroid functions were illustrated as ‘#’.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/33/07/178-1646159464-Figure4.jpg",
"caption": "Figure 4. Illustration of the correlations of iron, TIBC, Ferritin, TSH, and FT4 of 30 physically and clinically healthy women with their individual-corresponding vitamin D3 levels.",
"featured": false
}
],
"authors": [
{
"id": 271,
"affiliation": [
{
"affiliation": "School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, PR China"
}
],
"first_name": "Salauddin Al",
"family_name": "Azad",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-3035-7882",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 272,
"affiliation": [
{
"affiliation": "Department of Pharmacology, Jashore Medical College, Jashore, 7400, Bangladesh"
}
],
"first_name": "Sharmin",
"family_name": "Ahmed",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0003-1890-457X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 273,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Faculty of Biological Science and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh"
}
],
"first_name": "Partha",
"family_name": "Biswas",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-9766-756X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 274,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan, 25200, Malaysia"
}
],
"first_name": "Md. Abdur Rashid",
"family_name": "Mia",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 275,
"affiliation": [
{
"affiliation": "Department of Chemistry and Biochemistry, University of Oklahoma, Norman, 73019, USA"
}
],
"first_name": "Mithila",
"family_name": "Farjana",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-8145-7066",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 276,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Dhaka, 1229, Bangladesh"
}
],
"first_name": "Farzana Alam",
"family_name": "Arshe",
"email": null,
"author_order": 6,
"ORCID": "http://orcid.org/0000-0002-5510-3264",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 277,
"affiliation": [
{
"affiliation": "Ministry of Health, People’s Republic of Bangladesh"
}
],
"first_name": "Sabrina Jahan",
"family_name": "Mily",
"email": null,
"author_order": 7,
"ORCID": "http://orcid.org/0000-0002-1840-7613",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 278,
"affiliation": [
{
"affiliation": "Department of Gastroenterology, Square Hospitals LTD, Dhaka, 1205, Bangladesh"
}
],
"first_name": "Ananya Baidya",
"family_name": "Ankhi",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 279,
"affiliation": [
{
"affiliation": "Department of Mathematics and Natural Sciences, School of Data and Sciences, BRAC University, Dhaka, 1212, Bangladesh"
}
],
"first_name": "Mahdi Mubin",
"family_name": "Shaikat",
"email": null,
"author_order": 9,
"ORCID": "http://orcid.org/0000-0002-5570-7455",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 280,
"affiliation": [
{
"affiliation": "Enam Medical College and Hospital, Savar, Dhaka, 1340, Bangladesh"
}
],
"first_name": "Sabeeha",
"family_name": "Sultana",
"email": null,
"author_order": 10,
"ORCID": "http://orcid.org/0000-0001-9808-9645",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 281,
"affiliation": [
{
"affiliation": "Department Public Health & Bioinformatics, Asian University for Women, Chittagong, 4000, Bangladesh"
}
],
"first_name": "Kashfia",
"family_name": "Mawa",
"email": null,
"author_order": 11,
"ORCID": "http://orcid.org/0000-0002-3456-9110",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 282,
"affiliation": [
{
"affiliation": "Department of Animal Production and Management, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh"
}
],
"first_name": "Zannatul",
"family_name": "Naim",
"email": null,
"author_order": 12,
"ORCID": "http://orcid.org/0000-0002-2771-7576",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 283,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Immunology, Ibn-Sina hospital and Diagnostic Center, Jashore 7400, Bangladesh"
}
],
"first_name": "Riazul",
"family_name": "Islam",
"email": null,
"author_order": 13,
"ORCID": "http://orcid.org/0000-0001-5517-4874",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 284,
"affiliation": [
{
"affiliation": "Global Biotechnology & Biomedical Research Network (GBBRN), Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh."
},
{
"affiliation": "Department of Pathology, College of Korean Medicine, Kyung Hee University, Seoul 02447, South Korea"
},
{
"affiliation": "Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea"
}
],
"first_name": "Md. Ataur",
"family_name": "Rahman",
"email": null,
"author_order": 14,
"ORCID": "http://orcid.org/0000-0001-6649-3694",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 65
},
{
"id": 285,
"affiliation": [
{
"affiliation": "Department of Pathology, College of Korean Medicine, Kyung Hee University, Seoul 02447, South Korea"
},
{
"affiliation": "Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea"
}
],
"first_name": "Bonglee",
"family_name": "Kim",
"email": "bongleekim@khu.ac.kr",
"author_order": 15,
"ORCID": "http://orcid.org/0000-0002-8678-156X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Bonglee Kim, PhD; Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea, email: bongleekim@khu.ac.kr",
"article": 65
}
]
},
{
"id": 64,
"slug": "178-1646885559-human-immunodeficiency-virus-type-1-role-of-proteins-in-the-context-of-viral-life-cycle",
"type": "review_article",
"manuscript_id": "178-1646885559",
"published": "2022-04-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/19/178-1646885559.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 307-319.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Jha V, Rustagi K , et al. Human immunodeficiency virus type 1: Role of proteins in the\r\ncontext of viral life cycle. J Adv Biotechnol Exp Ther. 2022; 5(2): 307-319.",
"title": "Human immunodeficiency virus type 1: Role of proteins in the context of viral life cycle",
"abstract": "<p>The Acquired Immunodeficiency Syndrome (AIDS) is a major global pandemic and of paramount public health concern. Over the years, antiretroviral therapy (ART) has emerged as the gold standard of AIDS treatment. However, drug resistance and toxicity, and drug accessibility and compliance issues have blunted its positive impacts. Thirty years into the AIDS pandemic, there is a critical need of the hour to identify and develop an effective treatment strategy that can be successfully implemented with high levels of coverage. The HIV-1 life cycle bestows numerous potential targets for therapeutic intervention, however, only a few have been exploited till date. HIV- 1 encodes fifteen viral proteins from just nine genes. The expression of structural polyproteins Gag, Pol, and Env enable the assembly of the virion, the regulatory proteins Rev and Tat regulate viral gene expression and the accessory proteins Vpu, Nef, Vpr, and Vif equip the virion to evade or counteract host immune defenses. In this review article, we highlight each structural, regulatory, and accessory protein’s role in the context of the HIV-1 life cycle. We also discuss anti-retroviral therapy, its advantages, and shortcomings. Finally, we provide insight into various unexploited and potential therapeutic targets in the life cycle of HIV</p>",
"DOI": "10.5455/jabet.2022.d117",
"views": 1324,
"downloads": 128,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/35/07/178-1646885559-Figure1.jpg",
"caption": "Figure 1. Organization of the HIV-1 genome.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/35/07/178-1646885559-Figure2.jpg",
"caption": "Figure 2. Schematic overview of the HIV-1 life cycle (The viral proteins involved in each step are highlighted in red).",
"featured": false
}
],
"authors": [
{
"id": 218,
"affiliation": [
{
"affiliation": "National Facility for Biopharmaceutical, Guru Nanak Khalsa College, Nathelal Parekh Margh, Mumbai, Maharashtra 400019, India"
}
],
"first_name": "Vikas",
"family_name": "Jha",
"email": "vikasjjha7@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Vikas Jha, National Facility for Biopharmaceuticals, Guru Nanak\r\nKhalsa college, Nathelal Parekh Margh, Mumbai, Maharashtra 400019, India,\r\ne-mail: vikasjjha7@gmail.com",
"article": 64
},
{
"id": 219,
"affiliation": [
{
"affiliation": "Department of Biotechnology, University of Glasgow, Glasgow G12 8QQ, United Kingdom"
}
],
"first_name": "Kush",
"family_name": "Rustagi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 64
},
{
"id": 220,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular biology, Faculty of Biology and Chemistry, University of Bremen, Bibliothekstraße 1, 28359 Bremen, Germany"
}
],
"first_name": "Kunal",
"family_name": "Gharat",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 64
},
{
"id": 221,
"affiliation": [
{
"affiliation": "School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland"
}
],
"first_name": "Neetu",
"family_name": "Sonawane",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 64
},
{
"id": 222,
"affiliation": [
{
"affiliation": "School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland"
}
],
"first_name": "Meet",
"family_name": "Rathod",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 64
},
{
"id": 223,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Kishinchand Chellaram College Mumbai, Maharashtra 400020, India"
}
],
"first_name": "Reetikesh",
"family_name": "Patel",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 64
},
{
"id": 224,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Kishinchand Chellaram College Mumbai, Maharashtra 400020, India"
}
],
"first_name": "Sakshi",
"family_name": "Devkar",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 64
},
{
"id": 225,
"affiliation": [
{
"affiliation": "National Facility for Biopharmaceutical, Guru Nanak Khalsa College, Nathelal Parekh Margh, Mumbai, Maharashtra 400019, India"
}
],
"first_name": "Vrushali",
"family_name": "Damapurkar",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 64
},
{
"id": 226,
"affiliation": [
{
"affiliation": "National Facility for Biopharmaceutical, Guru Nanak Khalsa College, Nathelal Parekh Margh, Mumbai, Maharashtra 400019, India"
}
],
"first_name": "Navdeep",
"family_name": "Kaur",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 64
}
]
},
{
"id": 59,
"slug": "178-1641574027-hptlc-fingerprinting-analysis-of-phytoconstituents-from-bixa-orellana-and-beta-vulgaris-plant-pigment",
"type": "original_article",
"manuscript_id": "178-1641574027",
"published": "2022-04-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/36/178-1641574027.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 292-306.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Patel M, Patel P. HPTLC fingerprinting analysis of phytoconstituents from Bixa orellana and Beta vulgaris plant pigment. J Adv Biotechnol Exp Ther. 2022; 5(2): 292-306.",
"title": "HPTLC fingerprinting analysis of phytoconstituents from Bixa orellana and Beta vulgaris plant pigment",
"abstract": "<p>Pigments are a type of coloring component which are utilized by humans to enhance colors in their lives. Using synthetic pigments for the purpose of coloring food, clothes, fruit juices, paints are accepted worldwide previously, but due to hazardous impact of synthetic colors on environment, and on human health made to go for alternative sources of the pigments which are safe to use. Isolation of natural pigment is another preference that will increase the supply of pigment from natural sources while minimizing environmental and health risks. Thus, there is a growing necessity for biocolor derived from natural sources that can substitute synthetic colors. Natural colorants are commonly found from plants, animals, and microorganisms. Plant pigments have several benefits, so it seems much of prominence for pigment production. <em>Bixa orellana</em> and <em>Beta vulgaris</em> were isolated for yellow, orange, and red color pigments from natural ecological source. Pigment extraction from plants requires extract preparation and then isolation of pigments using different solvents. Extracted pigments were analyzed by preliminary screening techniques such as phytochemical assay and various confirmation tests. We found positive results for flavonoid, tannin, carbohydrate, protein, saponin and alkaloid using phytochemical assays. HPTLC fingerprinting was done for each extract and found positive result for alkaloid and phenolic compounds. Thus, it was aimed to develop the extraction analytical methods for determination of <em>Bixa Orellana</em> and <em>Beta Vulgaris</em> spp. by HPTLC fingerprint approach.</p>",
"DOI": "10.5455/jabet.2022.d116",
"views": 756,
"downloads": 121,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/07/178-1641574027-Figure1.jpg",
"caption": "Figure 1. Extraction of (A) Bixa Orellana, and (B) Beta vulgaris.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/07/178-1641574027-Figure2.jpg",
"caption": "Figure 2. Chromatograms of plant extract in HPTLC analysis. Alkaloid profile of (1. BVAE- Aqueous ethanol extract of Beta vulgaris, 2. BVAA- Aqueous acetone extract of Beta vulgaris, 3. BVM- Methanol extract of Beta vulgaris, 4. BVAM- Aqueous methanol extract of Beta vulgaris, 5. BOD- Dichloromethane extract of Bixa orellana, 6. BOM- Methanol extract of Bixa orellana, 7. BOA- Acetone extract of Bixa orellana, 8. BOC- Chloroform extract of Bixa orellana).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/07/178-1641574027-Figure3.jpg",
"caption": "Figure 3. HPTLC peak densitogram of alkaloid profile (at 366 nm) of Beta vulgaris and Bixa orellana plant extract (A. BVAE- Aqueous ethanol extract of Beta vulgaris, B. BVAA- Aqueous acetone extract of Beta vulgaris, C. BVM- Methanol extract of Beta vulgaris, D. BVAM- Aqueous methanol extract of Beta vulgaris, E. BOD- Dichloromethane extract of Bixa orellana, F. BOM- Methanol extract of Bixa orellana, G. BOA- Acetone extract of Bixa orellana, H. BOC- Chloroform extract of Bixa orellana).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/07/178-1641574027-Figure4.jpg",
"caption": "Figure 4. HPTLC peak densitogram of alkaloid profile (at 254 nm) of Beta vulgaris and Bixa orellana plant extract (A. BVAE- Aqueous ethanol extract of Beta vulgaris, B. BVAA- Aqueous acetone extract of Beta vulgaris, C. BVM- Methanol extract of Beta vulgaris, D. BVAM- Aqueous methanol extract of Beta vulgaris, E. BOD- Dichloromethane extract of Bixa orellana, F. BOM- Methanol extract of Bixa orellana, G. BOA- Acetone extract of Bixa orellana, H. BOC- Chloroform extract of Bixa orellana).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/07/178-1641574027-Figure5.jpg",
"caption": "Figure 5. Chromatograms of plant extract in HPTLC analysis. Phenolic profile of (1. BVAE- Aqueous ethanol extract of Beta vulgaris, 2. BVAA- Aqueous acetone extract of Beta vulgaris, 3. BVM- Methanol extract of Beta vulgaris, 4. BVAM- Aqueous methanol extract of Beta vulgaris, 5. BOD- Dichloromethane extract of Bixa orellana, 6. BOM- Methanol extract of Bixa orellana, 7. BOA- Acetone extract of Bixa orellana, 8. BOC- Chloroform extract of Bixa orellana).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/07/178-1641574027-Figure6.jpg",
"caption": "Figure 6. HPTLC peak densitogram of Phenolic profile (at 366 nm) of Beta vulgaris and Bixa orellana plant extract (A. BVAE- Aqueous ethanol extract of Beta vulgaris, B. BVAA- Aqueous acetone extract of Beta vulgaris, C. BVM- Methanol extract of Beta vulgaris, D. BVAM- Aqueous methanol extract of Beta vulgaris, E. BOD- Dichloromethane extract of Bixa orellana, F. BOM- Methanol extract of Bixa orellana, G. BOA- Acetone extract of Bixa orellana, H. BOC- Chloroform extract of Bixa orellana).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/07/178-1641574027-Figure7.jpg",
"caption": "Figure 7. HPTLC peak densitogram of phenolic profile (at 254 nm) of Beta vulgaris and Bixa orellana plant extract (A. BVAE- Aqueous ethanol extract of Beta vulgaris, B. BVAA- Aqueous acetone extract of Beta vulgaris, C. BVM- Methanol extract of Beta vulgaris, D. BVAM- Aqueous methanol extract of Beta vulgaris, E. BOD- Dichloromethane extract of Bixa orellana, F. BOM- Methanol extract of Bixa orellana, G. BOA- Acetone extract of Bixa orellana, H. BOC- Chloroform extract of Bixa orellana).",
"featured": false
}
],
"authors": [
{
"id": 216,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Mehsana Urban Institute of Sciences, Ganpat University, Mehsana, Gujarat, India"
}
],
"first_name": "Mrunali",
"family_name": "Patel",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 59
},
{
"id": 217,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Mehsana Urban Institute of Sciences, Ganpat University, Mehsana, Gujarat, India"
}
],
"first_name": "Priti",
"family_name": "Patel",
"email": "priti.patel@ganpatuniversity.ac.in",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Priti Patel, PhD; Department of Biotechnology, Mehsana Urban Institute of\r\nSciences, Ganpat University, Mehsana, Gujarat, India, email: priti.patel@ganpatuniversity.ac.in",
"article": 59
}
]
},
{
"id": 55,
"slug": "178-1641964368-evaluation-of-selective-mitis-salivarius-agar-for-the-isolation-of-streptococcus-mutans-and-its-resistance-pattern-in-bangladesh",
"type": "original_article",
"manuscript_id": "178-1641964368",
"published": "2022-04-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/20/178-1641964368.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 283-291.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Ferdose J, Alam MS, Tasnim A, et al. Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh. J Adv Biotechnol Exp Ther. 2022; 5(2): 283-291.",
"title": "Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh",
"abstract": "<p>Since <em>Streptococcus mutans</em> appears to be the most common cause of dental caries, appropriate laboratory media is necessary for the proper detection and management of this bacterium. The aim of this study was to evaluate the mitis salivarius agar (MSA) compared to conventional blood agar media (BAM) for detection of bacterium. This study was conducted in the Department of Microbiology of the Rajshahi Medical College, Rajshahi, Bangladesh from April 2017 to December 2017. The sample, dental swab was taken from 200 children, aged between 6-18 years who underwent dental caries and residing in Rajshahi district. All specimens were cultured to identify and compare the morphologic characteristics of its colonies both in MSA and BAM. In this study, antimicrobial susceptibility testing was also performed. This prospective observational study was conducted through regular and continuous monitoring of the results. Out of 200 specimens, the growth rate was 82%. Higher growth was observed in MSA (39.5%) than BAM (24.1%). Of them, we found 53.1% multi-drug resistant mutans. The most resistance was to Penicillin G (100%) followed by Azithromycin (95.3%). The study findings would help to increase the detection of mutans and its pattern for proper treatment towards the improvement of children’s dental health in Bangladesh.</p>",
"DOI": "10.5455/jabet.2022.d115",
"views": 1459,
"downloads": 129,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/51/07/178-1641964368-Figure1.jpg",
"caption": "Figure 1. Detection rate of mutans in different agar media.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/51/07/178-1641964368-Figure2.jpg",
"caption": "Figure 2. A. Growth of S. mutans on MSA, and B. Bacterial growth.",
"featured": false
}
],
"authors": [
{
"id": 190,
"affiliation": [
{
"affiliation": "Department of Pathology and Microbiology, National Institute of Diseases of the Chest and Hospital, Mohakhali, Dhaka, Bangladesh"
}
],
"first_name": "Jannatul",
"family_name": "Ferdose",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 55
},
{
"id": 191,
"affiliation": [
{
"affiliation": "Department of Microbiology, Rajshahi Medical College, Rajshahi, Bangladesh"
}
],
"first_name": "Md. Shah",
"family_name": "Alam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 55
},
{
"id": 192,
"affiliation": [
{
"affiliation": "Department of Microbiology, Parkview Medical College, Sylhet, Bangladesh"
}
],
"first_name": "Anika",
"family_name": "Tasnim",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 55
},
{
"id": 193,
"affiliation": [
{
"affiliation": "Molecular Genetics Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Rayhan",
"family_name": "Chowdhury",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 55
},
{
"id": 194,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Mohammad Nurul",
"family_name": "Matin",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 55
},
{
"id": 195,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Cell Biology, Bangladesh University of Health Sciences (BUHS), Mirpur, Dhaka 1216, Bangladesh"
}
],
"first_name": "Md. Mim",
"family_name": "Obaidullah",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 55
},
{
"id": 196,
"affiliation": [
{
"affiliation": "Institute of Biological Sciences, University of Rajshahi, Bangladesh"
}
],
"first_name": "Md. Abu",
"family_name": "Sayem",
"email": "sayem072003@yahoo.com",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Abu Sayem, PhD; Institute of Biological Sciences, University of Rajshahi, Rajshahi,Bangladesh, e-mail: sayem072003@yahoo.com",
"article": 55
}
]
},
{
"id": 88,
"slug": "178-1648617208-antimicrobial-and-phytochemical-screening-of-selected-wild-mushrooms-naturally-found-in-garhwal-himalayan-region-uttarakhand-india",
"type": "original_article",
"manuscript_id": "178-1648617208",
"published": "2022-03-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/59/178-1648617208.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 417-432.",
"academic_editor": "Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh",
"cite_info": "Kothiyal G, Singh K. Antimicrobial and phytochemical screening of selected wild mushrooms naturally found in Garhwal Himalayan region, Uttarakhand, India. J Adv Biotechnol Exp Ther. 2022; 5(2): 417-432.",
"title": "Antimicrobial and phytochemical screening of selected wild mushrooms naturally found in Garhwal Himalayan region, Uttarakhand, India",
"abstract": "<p>Natural products contain several ingredients that can treat number of ailments. Due to the increase in antibiotic-resistant microorganisms, natural resources are being looked at as an alternative source to combat harmful microbes. This can reduce the effects on harmful microbes by obtaining antibacterial compounds derived from natural resources. The aim of present study is to explore some new potent varieties of unexplored wild mushroom species to investigate their effects on microbial activity. In this study hexane, chloroform, methanol, 70% ethanol, and hot water extracts of <em>Cantharellus cibarius, Phellinus pectinatus, Laccaria laccata, Trametes versicolor</em>, and <em>Gloeophyllum sepiarium</em> were tested for antibacterial activity against nine bacterial strains namely <em>Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aerouginosa, Acinetobacter baumannii, Pseudomonas fluorescens, Enterobacter aerogenes, Proteus mirabilis</em> by disk diffusion method. The present study showing that <em>Phellinus pectinatus and Gloeophyllum sepiarium</em> mushroom species methanol and Ethanol extracts are most active against <em>Bacillus subtilis, Klebsiella pneumoniae, Acinetobacter baumannii, Enterobacter aerogenes</em> and <em>Pseudomonas aerouginosa</em> bacterial strains. The present study reveals important secondary metabolites compounds including alkaloids, flavonoids, carbohydrates, glycosides, etc. were present in wild mushroom extracts. Out of 5 extracts, methanol and ethanol extract have been shown a great potential as antimicrobial secondary metabolites compared to other extracts. The result of present research is expressing the high potency of extracts to stop the growth of bacteria and this extract can be further suggested for medical utilizations and could be used as natural antimicrobial source.</p>",
"DOI": "10.5455/jabet.2022.d125",
"views": 1589,
"downloads": 159,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1648617208-Figure1.jpg",
"caption": "Figure 1. Pictorial view of wild mushroom; A) Cantharellus cibarius, B) Phellinus pectinatus, C) Laccaria laccata, D) Trametes versicolor, and E) Gloeophyllum sepiarium.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1648617208-Figure2.jpg",
"caption": "Figure 2. 3D view of sample collection sites of Uttarakhand Himalayan region, India.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1648617208-Figure3.jpg",
"caption": "Figure 3. Extraction process of wild macrofungi (Mushrooms).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/54/07/178-1648617208-Figure4.jpg",
"caption": "Figure 4. Effect of different mushrooms extracts on microbial activity. (A) Cantharellus cibarius extracts against Bacillus subtilis, (B) Cantharellus cibarius extracts against Klebsiella pneumoniae, (c) Phellinus pectinatus extracts against Acinetobacter baumannii, (D) Phellinus pectinatus extracts against Klebsiella pneumoniae, (E) Laccaria laccata extracts against Pseudomonas aeruginosa, (F) Laccaria laccata extracts against Acinetobacter baumannii, (G) Trametes versicolor extracts against Pseudomonas aeruginosa, (H) Trametes versicolor extracts against Bacillus subtilis, (I) Gloeophyllum sepiarium extracts against Enterobacter aerogenes, (J) Gloeophyllum sepiarium extracts against Bacillus subtilis, (K) Streptomycin (S25mcg) against active strains Bacillus subtilis (L) Streptomycin (S25mcg) against active strains Acinetobacter baumannii, and (M) Streptomycin (S25mcg) against active strains Klebsiella pneumoniae.",
"featured": false
}
],
"authors": [
{
"id": 331,
"affiliation": [
{
"affiliation": "Department of Microbiology, SGRR School of Basic & Applied Sciences, SGRR University Dehradun-248001, Uttarakhand, India"
}
],
"first_name": "Gaurav",
"family_name": "Kothiyal",
"email": "gauravkothiyal790@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Gaurav Kothiyal, Department of Microbiology, SGRR School of Basic & Applied Sciences, SGRR University Dehradun-248001, Uttarakhand, India, e-mail: gauravkothiyal790@gmail.com",
"article": 88
},
{
"id": 332,
"affiliation": [
{
"affiliation": "Professor and Head Department of Microbiology, SGRR School of Basic & Applied Sciences, SGRR University Dehradun-248001, Uttarakhand, India"
}
],
"first_name": "Keerti",
"family_name": "Singh",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 88
}
]
},
{
"id": 54,
"slug": "178-1642348738-zoo-chemical-profiling-in-vivo-toxicity-and-in-vitro-anti-inflammatory-properties-of-luffariella-herdmani-marine-sponge-extract",
"type": "original_article",
"manuscript_id": "178-1642348738",
"published": "2022-03-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/05/178-1642348738.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 269-282.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Kuruppuarachchi SU, Gunathilake VK. Zoo-chemical profiling, in vivo toxicity and in vitro anti-inflammatory properties of Luffariella herdmani marine sponge extract. J Adv Biotechnol Exp Ther. 2022; 5(2): 269-282.",
"title": "Zoo-chemical profiling, in vivo toxicity and in vitro anti-inflammatory properties of Luffariella herdmani marine sponge extract",
"abstract": "<p>The present study investigates the zoo-chemical profiling, anti-inflammatory, and radical scavenging activities of <em>Luffariella herdmani</em> marine sponge extract. The sponge crude extract (SCE) was prepared by methanol/dichloromethane extraction, followed by rotary evaporation. The percentage yield was calculated, and the zoo-chemicals were investigated by standard methods, while anti-inflammatory activity was tested by protein denaturation assay. Radical scavenging activity of the SCE was tested against 2, 2-diphenyl-1-picrylhydrazy (DPPH), nitric oxide (NO) and peroxide radicals, while <em>in vivo</em> toxicity was evaluated by the <em>Artemia salina</em> lethality assay. The results indicated the presence of terpenoids, alkaloids, anthraquinones, unsaturated sterols, sterols, and saponins in the SCE while flavanoids, quinones, tannins, and phenols were absent. In vitro anti-inflammatory activity against protein denaturation with IC<sup>50</sup> of 58.54 µg/ml was evidenced while radical scavenging activity was not reported. The SCE was toxic to <em>A. salina</em> larvae with LC<sup>50</sup> of 14.34 µg/ml. In conclusion, <em>L. herdmani</em> sponge extract possesses <em>in vitro</em> anti-inflammatory, <em>in vivo</em> toxic properties, yet radical scavenging activity was absent. The presence of terpenoids, alkaloids, anthraquinones, unsaturated sterols, sterols, and saponins with reported anti-inflammatory properties is suggestive of the use of <em>L. herdmani</em> sponge extract as an anti-inflammatory drug lead.</p>",
"DOI": "10.5455/jabet.2022.d114",
"views": 1062,
"downloads": 162,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/21/07/178-1642348738-Figure1.jpg",
"caption": "Figure 1. Effect of different concentrations of (A) standard drug diclofenac sodium and (B) SCE on egg albumin denaturation (Pearson, r = 0.973, p = 0.005 and r = 0.985, p = 0.002, respectively). Data are presented as mean ± SEM (n=3).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/21/07/178-1642348738-Figure2.jpg",
"caption": "Figure 2. Effect of different concentrations of (A) standard drug ascorbic acid and (B) SCE on DPPH scavenging activity (Pearson, r = 0.888, p = 0.044, r = -0.915, p = 0.004, respectively). Data are presented as mean ± SEM (n=3).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/21/07/178-1642348738-Figure3.jpg",
"caption": "Figure 3. Effect of different concentrations of (A) standard drug ascorbic acid and (B) SCE on NO scavenging activity (Pearson, r = 0.898, p = 0.039 and r = -0.981, p = 0.000, respectively). Data are presented as mean ± SEM (n=3).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/21/07/178-1642348738-Figure4.jpg",
"caption": "Figure 4. Effect of different concentrations of (A) standard drug ascorbic acid and (B) SCE on peroxide scavenging activity (Pearson, r = 0.918, p = 0.028 and r = -0.993, p = 0.000, respectively). Data are presented as mean ± SEM (n=3).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/21/07/178-1642348738-Figure5.jpg",
"caption": "Figure 5. Correlation of percentage mortality of A. salina larvae after exposing to different concentrations of SCE (Pearson, r = 0.986, p = 0.002). Data are presented as mean ± SEM (n=3).",
"featured": false
}
],
"authors": [
{
"id": 170,
"affiliation": [
{
"affiliation": "Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka"
}
],
"first_name": "Sashini Umasha",
"family_name": "Kuruppuarachchi",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-8409-4571",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 54
},
{
"id": 171,
"affiliation": [
{
"affiliation": "Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka"
}
],
"first_name": "Varuni Karunika",
"family_name": "Gunathilake",
"email": "varunig@sjp.ac.lk",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-8409-4571",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Varuni Karunika Gunathilake, PhD; Department of Zoology, Faculty of\r\nApplied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka, e-mail: varunig@sjp.ac.lk",
"article": 54
}
]
},
{
"id": 53,
"slug": "178-1640719270-pathological-investigation-and-molecular-detection-of-bacterial-zoonotic-diseases-of-slaughtered-cattle-in-bangladesh",
"type": "original_article",
"manuscript_id": "178-1640719270",
"published": "2022-02-17",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/29/178-1640719270.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(2): 257-268.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Sultana N, Pervin M, Sultana S, et al. Pathological investigation and molecular detection of bacterial zoonotic diseases of slaughtered cattle in Bangladesh. J Adv Biotechnol Exp Ther. 2022; 5(2): 257-268.",
"title": "Pathological investigation and molecular detection of bacterial zoonotic diseases of slaughtered cattle in Bangladesh",
"abstract": "<p>The research was undertaken to investigate the important bacterial zoonotic diseases in slaughtered cattle in Mymensingh district of Bangladesh. The targeted diseases were tuberculosis (TB), leptospirosis, listeriosis, and brucellosis. Samples (mesenteric lymph nodes, lungs, and liver) were collected from 50 slaughtered cattle from different slaughterhouses in Mymensingh district during the periods from October 2019 to November 2021. The diagnosis was made based on gross pathological findings and histopathology by hematoxylin and eosin staining and acid-fast staining. The confirmatory diagnosis was done by polymerase chain reaction using disease-specific primers. Grossly, calcifications and caseation of mesenteric lymph nodes, caseous nodule formation in the liver and lungs, and enlarged mesenteric lymph nodes were the predominant lesions seen. Histopathologically, caseous necrosis and calcification surrounded by fibrous connective tissues in the mesenteric lymph nodes, and granuloma mixed with acid-fast bacteria in the liver were seen as suggestive of infectivity due to TB. Marked lymphoid depletion was seen in listeriosis suspected cases. The PCR amplified <em>Mycobacterium tuberculosis complex </em>(372 bp/16S rRNA), <em>M. bovis </em>(MPB83/600 bp),<em> Leptospira interrogans </em>serovar Hardjoprajitno (HP/323 bp) and <em>Listeria monocytogenes</em> (<em>InlC</em>/517 bp) species-specific amplicons in 09, 09, 05, and 05 cattle, respectively. Brucellosis was not identified in any cases in this study. In conclusion, deadly zoonotic diseases (TB, leptospirosis, and listeriosis) are present in slaughtered cattle having public health importance. Therefore, more extensive monitoring and epidemiological surveys are necessary for the effective prevention and control of zoonotic diseases of cattle.</p>",
"DOI": "10.5455/jabet.2022.d113",
"views": 1425,
"downloads": 206,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/38/07/178-1640719270-Figure1.jpg",
"caption": "Figure 1. Study layout.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/38/07/178-1640719270-Figure2.jpg",
"caption": "Figure 2. Examination of mesenteric lymph nodes, lungs and liver of slaughtered cattle. A. Swollen and enlarged mesenteric lymph nodes were seen, and hemorrhages were seen at the cut surface (inset). B. Caseous materials (arrows) were seen following cross section of the mesenteric lymph node. C. Congested lung (arrow) and D. Cirrhosis (arrows) were seen. Bar = 2 cm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/38/07/178-1640719270-Figure3.jpg",
"caption": "Figure 3. Histopathology of the mesenteric lymph nodes, lungs, and liver of slaughtered cattle. A. Caseous necrosis (asterisk) surrounded by fibrous connective tissues (arrow) and accompanied by mononuclear cells (arrowhead) was seen in the mesenteric lymph node B. Calcification (arrow) surrounded by fibrous connective tissues accompanied by infiltrates with mononuclear cells and langhan’s types giant cells were seen (inset: higher magnification). C. Multifocal Granuloma (arrows) were seen in the lungs. D. Granuloma (arrow) characterized by infiltrates with lymphocytes and foamy macrophages as well as hemorrhages (arrowheads) along with deposition of hemosiderin pigments (asterisk) were seen in the liver and in higher magnification (E). F: Acid-fast bacteria were seen in the macrophages or in the epithelioid cells of the granulomas and its surrounding tissues. H & E stain (A-E); acid-fast stain (F). Bar (A-D = 200 µm; B (inset) and E-F = 100 µm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/38/07/178-1640719270-Figure4.jpg",
"caption": "Figure 4. A. PCR amplified products of 372 bp fragments of the 16S rRNA gene of Mycobacterium tuberculosis complex isolates from cattle. L = DNA marker (100 bp), PC = Positive control, NC = Negative control, lane 01-03 = representative M. tuberculosis complex isolates from the liver, lane 05-06 = representative M. tuberculosis complex isolates from the lungs, lane 08-11 = representative M. tuberculosis complex isolates from the mesenteric lymph nodes. B. PCR amplified products of 600 bp fragments of the MPB83 gene of Mycobacterium bovis isolates from cattle. L = DNA marker (100 bp), PC = Positive control, NC= Negative control, lane 01-03 = representative M. bovis isolates from the liver, lane 04-05 = representative M. bovis isolates from the lungs, lane 06-09 = representative M. bovis isolates from the mesenteric lymph nodes.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/38/07/178-1640719270-Figure5.jpg",
"caption": "Figure 5. A. PCR amplified products of 323 bp fragments of the HP gene of Leptospira interrogans serovar Hardjoprajitno isolates from cattle. L = DNA marker (100 bp), NC= Negative control, lane 01-05 = representative L. interrogans serovar Hardjoprajitno isolates from the mesenteric lymph nodes of cattle. B. PCR amplified products of 517 bp fragments of the InlC gene of Listeria monocytogenes isolates from cattle. L = DNA marker (100 bp), NC= Negative control, lane 04-07 = representative L. monocytogenes isolates from the mesenteric lymph nodes of cattle.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/38/07/178-1640719270-Figure6.jpg",
"caption": "Figure 6. The percentages of tuberculosis (TB), leptospirosis, listeriosis and brucellosis in naturally infected cattle. *p = 0.024 indicates percentage of TB was significantly higher than other organisms in cattle.",
"featured": false
}
],
"authors": [
{
"id": 164,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Nazneen",
"family_name": "Sultana",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-8060-4805",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 53
},
{
"id": 165,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Munmun",
"family_name": "Pervin",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-5514-5798",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 53
},
{
"id": 166,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sajeda",
"family_name": "Sultana",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-3902-9447",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 53
},
{
"id": 167,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Moutuza",
"family_name": "Mostaree",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0003-1380-908X",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 53
},
{
"id": 168,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "S M Shariful Hoque",
"family_name": "Belal",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-6374-2154",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 53
},
{
"id": 169,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mohammad Abu Hadi Noor Ali",
"family_name": "Khan",
"email": "hadi.khan@bau.edu.bd",
"author_order": 6,
"ORCID": "http://orcid.org/0000-0002-8409-4571",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Abu Hadi Noor Ali Khan, PhD; Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, e-mail: hadi.khan@bau.edu.bd",
"article": 53
}
]
}
]
},
{
"id": 16,
"title": "Vol5 Issue1",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2022",
"slug": "2022-vol5-issue1",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 46,
"slug": "178-1638784882-covid-19-drugs-a-necessary-strategy-for-living-with-covid-19-in-the-new-normal-and-the-mutations-of-the-sars-cov-2-such-as-omicron-variant",
"type": "review_article",
"manuscript_id": "178-1638784882",
"published": "2022-01-17",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/34/178-1638784882.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 241-246.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "chu DT, Bui NL, Thi YVN, et al. COVID-19 drugs: A necessary strategy for living with COVID-19 in the new normal and the mutations of the SARS-CoV-2 such as omicron variant. J Adv Biotechnol Exp Ther. 2022; 5(1): 241-246.",
"title": "COVID-19 drugs: A necessary strategy for living with COVID-19 in the new normal and the mutations of the SARS-CoV-2 such as omicron variant",
"abstract": "<p>The COVID-19 pandemic has spread rapidly and caused significant damage to global public health as well as the economy. While the race of vaccine development witnessed a spectacular breakthrough with the introduction of highly effective vaccines such as BNT162b2, mRNA-1273, or AZD1222, no specific drug for COVID-19 treatment has been discovered yet. Recently, repurposing drugs classified into three main groups of mechanisms, including antivirus, anti-SARS-CoV-2 antibodies, and immunomodulators, are investigated. As a result, Remdesivir and six other drugs are authorized by the Food and Drug Administration (FDA) to treat patients infected by the SARS-CoV-2. This work aims to highlight that, besides vaccines, COVID-19 drugs should get more attention and be considered as one of the most promising strategies to safely coexist with the SARS-CoV-2 virus in the new normal status and for the mutations of the virus, such as the omicron variant.</p>",
"DOI": "10.5455/jabet.2022.d111",
"views": 387,
"downloads": 110,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/50/29/178-1638784882-Figure1.jpg",
"caption": "Figure 1. SARS-CoV-2 virus’s life circle and drug targets. Antibodies, IL: Interleukin, RNA: ribose nucleotide acid, RdRp: RNA-dependent RNA polymerase. Create with Biorender.com.",
"featured": false
}
],
"authors": [
{
"id": 152,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Dinh-Toi",
"family_name": "chu",
"email": "chudinhtoi.hnue@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dinh-Toi Chu, PhD; Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam, e-mail: chudinhtoi.hnue@gmail.com",
"article": 46
},
{
"id": 153,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Nhat-Le",
"family_name": "Bui",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 46
},
{
"id": 154,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Yen-Vy Nguyen",
"family_name": "Thi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 46
},
{
"id": 155,
"affiliation": [
{
"affiliation": "Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam"
}
],
"first_name": "Suong-Mai Vu",
"family_name": "Ngoc",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 46
}
]
},
{
"id": 52,
"slug": "178-1636169522-prevalence-of-hyperlipidemia-in-controlled-and-uncontrolled-type-2-diabetic-patients",
"type": "original_article",
"manuscript_id": "178-1636169522",
"published": "2022-01-17",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1636169522.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 247-256.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Hasnat MA, Niloy FR, Ashik AI, et al. Prevalence of hyperlipidemia in controlled and uncontrolled type-2 diabetic patients. J Adv Biotechnol Exp Ther. 2022; 5(1): 247-256.",
"title": "Prevalence of hyperlipidemia in controlled and uncontrolled type-2 diabetic patients",
"abstract": "<p>Patients with type-2 diabetes mellitus (T2DM) are known to suffer from hyperlipidemia. How hyperlipidemia is associated with controlled and uncontrolled T2DM patients in Bangladesh remained to be addressed. In this cross-sectional study, 211 participants were enrolled who have been suffering from T2DM for more than 4 years from the northeastern part of Bangladesh. Controlled and uncontrolled patients were defined with their plasma glycated hemoglobin (HbA1c) levels. Among them, 39% and 61% were in the diabetic-controlled and uncontrolled groups. Indeed, the diabetic uncontrolled group showed a higher frequency of hypercholesterolemia, hypertriglyceridemia, hyper LDL-cholesterolemia, and hypo HDL-cholesterolemia compare to the diabetic controlled group. Lipid profiling analysis revealed significantly elevated (p<0.0001) levels of cholesterol, triglyceride, and low-density lipoprotein (LDL) in uncontrolled than the controlled group, while high-density lipoprotein (HDL) was significantly (p<0.0001) lower in uncontrolled diabetics patients. Interestingly, significantly (p<0.05) higher dyslipidemia was also observed in individuals with controlled diabetic population, who have been suffering from T2DM for more than 7 years. Therefore, these results highlight that not only the diabetic uncontrolled but also the controlled group patients have a high risk of developing hyperlipidemia after a certain period of diabetes.</p>",
"DOI": "10.5455/jabet.2022.d112",
"views": 1707,
"downloads": 359,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/06/30/178-1636169522-Figure1.jpg",
"caption": "Figure 1. (A) The score of HbA1c between controlled and uncontrolled diabetic patients, which is significant using student t-test, p<0.0001. (B) Appearance of diabetes mellitus in several age groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/06/30/178-1636169522-Figure2.jpg",
"caption": "Figure 2. Amplitude of dyslipidemia in diabetic patients.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/06/30/178-1636169522-Figure3.jpg",
"caption": "Figure 3. Lipid profile analysis of both controlled and uncontrolled diabetic patients. (A), (B), (C), cholesterol, triglyceride, and LDL levels in uncontrolled diabetic patients were significantly (p <0.0001) higher than in the controlled group. (D) HDL level was significantly (p <0.0001) lower in the uncontrolled group than in the controlled one.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/06/30/178-1636169522-Figure4.jpg",
"caption": "Figure 4. Study of lipid profile in the diabetic controlled group with onset of T2DM duration (year) manner. (A), (B), significantly elevated cholesterol and triglyceride were found in the diabetic controlled group after 7 (p<0.05) and 8 (p<0.01) years but not before 6 and 7 years of T2DM onset. (C), significantly (p<0.05) increased LDL was found after 8 years but not earlier. (D), Significantly lower HDL was found after 7 (p<0.05) and 8 (p<0.01) years but not prior to 6 and 7 years of T2DM onset.",
"featured": false
}
],
"authors": [
{
"id": 156,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Mohammad Abul",
"family_name": "Hasnat",
"email": "hasnat-bmb@sust.edu",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Abul Hasnat, Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh, e-mail: hasnat-bmb@sust.edu",
"article": 52
},
{
"id": 157,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Farhan Rahman",
"family_name": "Niloy",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 52
},
{
"id": 158,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Arafat Islam",
"family_name": "Ashik",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 52
},
{
"id": 159,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Mahedi",
"family_name": "Hasan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 52
},
{
"id": 160,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Preonath Chondrow",
"family_name": "Dev",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 52
},
{
"id": 161,
"affiliation": [
{
"affiliation": "Food and Nutrition, Ibn Sina Hospital Sylhet Lit., Sylhet, Bangladesh"
}
],
"first_name": "Sharmin Sultana",
"family_name": "Panna",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 52
},
{
"id": 162,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Md. Waseque",
"family_name": "Mia",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 52
},
{
"id": 163,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Zafrul",
"family_name": "Hasan",
"email": "zafrul-bmb@sust.edu",
"author_order": 8,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Zafrul Hasan, Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh, e-mail: zafrul-bmb@sust.edu",
"article": 52
}
]
},
{
"id": 43,
"slug": "178-1634619354-aspergillus-niger-grows-faster-than-escherichia-coli-in-eosin-methylene-blue-media-and-deter-their-growth-by-reducing-the-ph-of-the-media",
"type": "original_article",
"manuscript_id": "178-1634619354",
"published": "2021-12-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/45/178-1634619354.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 229-240.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Hossain MI, Ali MS. Aspergillus niger grows faster than Escherichia coli in eosin methylene blue media and deter their growth by reducing the pH of the media. J Adv Biotechnol Exp Ther. 2022; 5(1): 229-240.",
"title": "Aspergillus niger grows faster than Escherichia coli in eosin methylene blue media and deter their growth by reducing the pH of the media",
"abstract": "<p>Fungi is a kingdom that includes multicellular eukaryotic organisms such as yeast and mold; these organisms are heterotrophs (cannot make their own food) but have significant roles in nutrient cycling. To obtain nutrients from organic material, they use their hyphae, which elongate and branch off swiftly; using the mycelium quickly, they increase their size. Currently, a few media are suitable for fungal growth, such as sabouraud dextrose, malt extract and brain heart infusion medium. Bacterial eosin methylene blue (EMB) media is well-suited to fungi, which acts as selective media to differentiate Gram-negative bacteria. EMB, known as “Levine’s formulation”, is a selective and differential medium for Gram-negative bacteria. In EMB media, fungi even grow faster than Gram-negative bacteria. In addition to this faster growth of fungi, it deters the growth of Gram-negative bacteria by reducing the pH. The majority of the time, fungi require specific conditions to flourish. In this study, we observed fungal growth, especially mold (<em>Aspergillus niger</em>), in EMB media and its retardation activity of Gram-negative bacterial growth. For this new finding assurance, we performed the bacterial and fungal identification test further along with repeating the three times of the whole experiment, and we found the same result. The fungal species was <em>A. niger</em>, and the bacterial species was <em>Escherichia coli</em>.</p>",
"DOI": "10.5455/jabet.2022.d110",
"views": 2406,
"downloads": 140,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/30/178-1634619354-Figure1.jpg",
"caption": "Figure 1. A simple illustration of the MTF technique for coliform identification.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/30/178-1634619354-Figure2.jpg",
"caption": "Figure 2. Sample growth after 48 h of incubation soil sample-A (A); Washroom sample-B (B); canal water sample-C (C); E. coli (non-spore forming, Gram-negative and rod shaped) bacteria after 72 h (D); A. niger (an infectious agent commonly found on mucosal surface, gastrointestinal tract and human skin) fungi after 72 h (E).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/36/30/178-1634619354-Figure3.jpg",
"caption": "Figure 3. A) pH value of soil, washroom and canal samples. B) Colony numbers of soil, washroom and canal samples. C) pH value of Gram-negative bacteria and fungal samples. D) Colony numbers of Gram-negative bacteria and fungal samples. Different letters on bars indicate significant differences at p < 0.05.",
"featured": false
}
],
"authors": [
{
"id": 128,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj8100, Bangladesh"
}
],
"first_name": "Md. Imran",
"family_name": "Hossain",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-6523-9958",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 43
},
{
"id": 129,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj8100, Bangladesh"
}
],
"first_name": "Md. Sarafat",
"family_name": "Ali",
"email": "sarafatbiotech@bsmrstu.edu.bd",
"author_order": 2,
"ORCID": "http://orcid.org/0000-0003-1969-7883",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Sarafat Ali, PhD; Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and\r\nTechnology University, Gopalganj8100, Bangladesh, email: sarafatbiotech@bsmrstu.edu.bd",
"article": 43
}
]
},
{
"id": 42,
"slug": "178-1634746160-syzygium-aromaticum-as-a-possible-source-of-sars-cov-2-main-protease-inhibitors-evidence-from-a-computational-investigation",
"type": "original_article",
"manuscript_id": "178-1634746160",
"published": "2021-12-14",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/56/178-1634746160.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 218-228.",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Ali MC, Nur AJ, Hasib RA, et al. Syzygium aromaticum as a possible source of SARS-CoV-2 main protease inhibitors: Evidence from a computational investigation. J Adv Biotechnol Exp Ther. 2022; 5(1): 218-228.",
"title": "Syzygium aromaticum as a possible source of SARS-CoV-2 main protease inhibitors: Evidence from a computational investigation",
"abstract": "<p>SARS-CoV-2, a new and fast circulating coronavirus strain, infected over 214 countries and territories worldwide and caused global health emergencies. The absence of appropriate medicines and vaccinations has further complicated the condition. SARS-CoV-2 main protease (M<sup>pro</sup>) is crucial for its propagation, and it is considered a striking target. This study used several computational approaches to determine the probable antagonist of SARS-CoV-2 M<sup>pro</sup> from bioactive phytochemicals of <em>Syzygium aromaticum</em>. A total of 20 compounds were screened through <em>in silico</em> approach. The molecular dynamics simulation studies were then carried out for further insights. We found crategolic acid, oleanolic acid, and kaempferol have considerable binding affinity and important molecular contacts with catalytic pocket residues, His41-Cys145. The pharmacological properties through ADMET analysis also showed that these compounds could be used as safe drug candidates. The molecular dynamics simulation study further confirmed these compound’s stability with M<sup>pro</sup>. However, further detailed <em>in-vitro</em> and <em>in-vivo</em> analyses are compulsory to evaluate the real potentiality of identified compounds.</p>",
"DOI": "10.5455/jabet.2022.d109",
"views": 1356,
"downloads": 218,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/30/178-1634746160-Figure1.jpg",
"caption": "Figure 1. Molecular interactions of selected compounds with SARS-Cov-2 Mpro (a) Positive control α-ketoamide, (b) Crategolic acid, (c) Oleanolic acid, and (d) Kaempferol.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/30/178-1634746160-Figure2.jpg",
"caption": "Figure 2. Molecular dynamics simulation studies. (a) Root Mean Square Deviation analysis; (b) Radius of gyration analysis, (c) Root Mean Square Fluctuation analysis, (d) Solvent accessible surface area analysis, (e) Number of Hydrogen bond analysis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/15/30/178-1634746160-Figure3.jpg",
"caption": "Figure 3. A graphical representation of the study. The screened compounds could potentially inhibit the activity of SARS-CoV-2.",
"featured": false
}
],
"authors": [
{
"id": 116,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Md. Chayan",
"family_name": "Ali",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-5668-2176",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 42
},
{
"id": 117,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Anjumana Jannati",
"family_name": "Nur",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 42
},
{
"id": 118,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Rizone Al",
"family_name": "Hasib",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 42
},
{
"id": 119,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Rubaeit All",
"family_name": "Rakib",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 42
},
{
"id": 120,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Mst. Shanzeda",
"family_name": "Khatun",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 42
},
{
"id": 121,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Md Mafizur",
"family_name": "Rahman",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 42
},
{
"id": 122,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, Bangladesh"
}
],
"first_name": "Md. Shahedur",
"family_name": "Rahman",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 42
},
{
"id": 123,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Md. Khasrul",
"family_name": "Alam",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 42
},
{
"id": 124,
"affiliation": [
{
"affiliation": "Department of Electrical and Electronic Engineering, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Md. Abdullah Al",
"family_name": "Mashud",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 42
},
{
"id": 125,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Mohammad Abu Hena Mostofa",
"family_name": "Jamal",
"email": "jamalbtg@gmail.com",
"author_order": 10,
"ORCID": "http://orcid.org/0000-0003-0542-4323",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Abu Hena Mostofa Jamal, Department of Biotechnology and\r\nGenetic Engineering, Faculty of Biological Sciences, Islamic University,\r\nKushtia 7003, Bangladesh, email: jamalbtg@gmail.com",
"article": 42
}
]
},
{
"id": 41,
"slug": "178-1633436612-mucormycosis-black-fungus-and-its-impact-on-the-covid-19-patients-an-updated-review",
"type": "review_article",
"manuscript_id": "178-1633436612",
"published": "2021-11-28",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/47/178-1633436612.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 198-217.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Huq AKMM, Hossain MG, Islam MS, et al. Mucormycosis (black fungus) and its impact on the COVID-19 patients: An updated review. J Adv Biotechnol Exp Ther. 2022; 5(1): 198-217.",
"title": "Mucormycosis (black fungus) and its impact on the COVID-19 patients: An updated review",
"abstract": "<p>Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global pandemic of the century. The disease is wreaking havoc on human health, the world economy, society, and the environment. It has already caused the loss of millions of lives. Because of the mutation, the virus is constantly evolving itself, changing its nature including the disease transmission rate, virulence, pathogenesis, and clinical manifestations. It was recently reported that certain COVID-19 patients are also suffering from a fungal infection as co-infection commonly known as mucormycosis (black fungus). In India, the outbreak of black fungus in COVID-19 patients has already been declared an epidemic. Only a few reports are noticed in other countries. The focus must now be put toward better management and control of the COVID-19-associated fungal infection. In this review, we have discussed various aspects of black fungus particularly the etiology, taxonomy, risk factors, transmission, pathogenesis, clinical manifestations, diagnosis, and line of treatment to keep up to date on how to manage this fungal infection better.</p>",
"DOI": "10.5455/jabet.2022.d108",
"views": 1230,
"downloads": 129,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/57/04/178-1633436612-Figure1.jpg",
"caption": "Figure 1. Most common and important risk factors associated with mucormycosis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/57/04/178-1633436612-Figure2.jpg",
"caption": "Figure 2. Transmission patterns of mucormycosis to humans.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/57/04/178-1633436612-Figure3.jpg",
"caption": "Figure 3. Pathogenesis of COVID-19 associated mucormycosis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/57/04/178-1633436612-Figure4.jpg",
"caption": "Figure 4. Countries reported for the COVID-19 associated mucormycosis (CAM) patients.",
"featured": false
}
],
"authors": [
{
"id": 110,
"affiliation": [
{
"affiliation": "Department of Pharmacy, University of Asia Pacific, Dhaka, Bangladesh"
}
],
"first_name": "AKM Moyeenul",
"family_name": "Huq",
"email": null,
"author_order": 1,
"ORCID": "http://orcid.org/0000-0001-94379821",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 41
},
{
"id": 111,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Golzar",
"family_name": "Hossain",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0002-1487-5444",
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 41
},
{
"id": 112,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Saiful",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0002-6870-4595",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 41
},
{
"id": 113,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Abdus",
"family_name": "Sobur",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0003-2457-6162",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 41
},
{
"id": 114,
"affiliation": [
{
"affiliation": "Naogaon District Hospital. Naogaon, Bangladesh."
}
],
"first_name": "AMM Taufiquer",
"family_name": "Rahman",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 41
},
{
"id": 115,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Tanvir",
"family_name": "Rahman",
"email": "tanvirahman@bau.edu.bd",
"author_order": 6,
"ORCID": "http://orcid.org/0000-0001-5432-480X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Tanvir Rahman, PhD; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University,Mymensingh-2202, Bangladesh, e-mail: tanvirahman@bau.edu.bd",
"article": 41
}
]
},
{
"id": 40,
"slug": "178-1634070556-nata-as-a-source-of-dietary-fiber-with-numerous-health-benefits",
"type": "review_article",
"manuscript_id": "178-1634070556",
"published": "2021-11-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/04/178-1634070556.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 189-197.",
"academic_editor": "Akhi Moni, PhD; ABEx Bio-Research Center, Bangladesh",
"cite_info": "Tallei TE, MS, Abas AH, et al. Nata as a source of dietary fiber with numerous health benefits.J Adv Biotechnol Exp Ther. 2022; 5(1): 189-197.",
"title": "Nata as a source of dietary fiber with numerous health benefits",
"abstract": "<p>In recent years, the general public’s perception of modern diets and human health has shifted dramatically. Changes in lifestyle make people prefer fast food that is poor in nutrients, especially dietary fiber. Lack of dietary fiber is one of the contributions to the increasing prevalence of non-communicable diseases, such as hypercholesterolemia and cancer, among the public. For this reason, efforts to introduce fiber-rich functional foods need to be encouraged. Nata, which is a high-fiber food made from organic plant sources, can be used as an alternative source of fiber for the community. It is hoped that this article will provide some insight into alternative sources of dietary fiber that can be produced by members of the community on their own.</p>",
"DOI": "10.5455/jabet.2022.d107",
"views": 6430,
"downloads": 208,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/02/178-1634070556-Figure1.jpg",
"caption": "Figure 1. The structure of cellulose (m = 2,000 - 26,000). Each monomer unit is β-D-glucose, and each beta acetal link connects the carbon atom 1 of one glucose to the carbon atom 4 of the next glucose.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/02/178-1634070556-Figure2.jpg",
"caption": "Figure 2. Nata de coco piña.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/02/178-1634070556-Figure3.jpg",
"caption": "Figure 3. The flowchart of nata de coco piña processing.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/02/178-1634070556-Figure4.jpg",
"caption": "Figure 4. Schematic diagram of summary of the study. Juice made of organic wastes is subjected for fermentation with A. xylinum. These provide various kinds of natas. These natas are the potential sources of insoluble fiber and other essential micronutrients with many health benefits.",
"featured": false
}
],
"authors": [
{
"id": 101,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Trina Ekawati",
"family_name": "Tallei",
"email": "trina_tallei@unsrat.ac.id",
"author_order": 1,
"ORCID": "http://orcid.org/0000-0002-7963-7527",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Trina Ekawati Tallei, PhD; Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia. e-mail: trina_tallei@unsrat.ac.id",
"article": 40
},
{
"id": 102,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Siti",
"family_name": "Marfuah",
"email": null,
"author_order": 2,
"ORCID": "http://orcid.org/0000-0001-7587-2055",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 40
},
{
"id": 103,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Abdul Hawil",
"family_name": "Abas",
"email": null,
"author_order": 3,
"ORCID": "http://orcid.org/0000-0001-9558-4739",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 40
},
{
"id": 104,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Andi Amelia Dwi Putri",
"family_name": "Abram",
"email": null,
"author_order": 4,
"ORCID": "http://orcid.org/0000-0001-9941-2555",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 40
},
{
"id": 105,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Nelsyani",
"family_name": "Pasappa",
"email": null,
"author_order": 5,
"ORCID": "http://orcid.org/0000-0002-5360-6239",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 40
},
{
"id": 106,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Putri Sri",
"family_name": "Anggini",
"email": null,
"author_order": 6,
"ORCID": "http://orcid.org/0000-0002-9759-3812",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 40
},
{
"id": 107,
"affiliation": [
{
"affiliation": "Department of Management, Faculty of Economics and Business, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Agus Supandi",
"family_name": "Soegoto",
"email": null,
"author_order": 7,
"ORCID": "http://orcid.org/0000-0002-4172-0018",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 40
},
{
"id": 108,
"affiliation": [
{
"affiliation": "Pharmacy Study Program, Faculty of Economics and Business, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Fatima",
"family_name": "Wali",
"email": null,
"author_order": 8,
"ORCID": "http://orcid.org/0000-0003-2480-5420",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 40
},
{
"id": 109,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh"
}
],
"first_name": "Talha Bin",
"family_name": "Emran",
"email": "talhabmb@bgctub.ac.bd",
"author_order": 9,
"ORCID": "http://orcid.org/0000-0003-3188-2272",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran, PhD; Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh, e-mail: talhabmb@bgctub.ac.bd",
"article": 40
}
]
},
{
"id": 39,
"slug": "178-1628892758-molecular-and-immunological-activity-of-terminalia-chebula-extracts",
"type": "original_article",
"manuscript_id": "178-1628892758",
"published": "2021-11-09",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/36/178-1628892758.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 176-188.",
"academic_editor": "",
"cite_info": "Al-Mousawi HTM, Mushtaq ABN, Bohan AH. Molecular and immunological activity of Terminalia chebula extracts. J Adv Biotechnol Exp Ther. 2022; 5(1): 176-188.",
"title": "Molecular and immunological activity of Terminalia chebula extracts",
"abstract": "<p>Several plant extracts and Ayurvedic formulations were used to treat ailments and such studies were well documented within the recent decades. Even a number of the plants were screened for immunomodulators to revive and rejuvenate the system. The present study is a screening trail for any possible healing activity of <em>Terminalia chebula</em> on IL-2 and IFN-γ levels. The raw and dried fruits of the sample were pulverized finely and extracted with methanol. Following which their aqueous solutions are re extracted with hexane, ester and chloroform to review the possible cytotoxic effects. Lipopolysaccharide (LPS)-stimulated macrophage cells were used throughout the study to assess the effect of extracts on nitric oxide (NO) production using Griess method. Expression of Cyclooxygenase-2 (COX2) and tumor necrosis factor (TNF)-α were studied by real time PCR quantification alongside estimation of IL-1β and IL-6 cytokine levels using the enzyme-linked immunosorbent assay (ELISA). The present study confirmed the positive effect of the chloroform extract in reducing the NO secretion and also by showing an inhibition within the expression of COX2, IL-1β, IL-6, and TNF-α. Thus, to conclude <em>T. chebula</em> might be used as anti-inflammatory candidate drug and also be used additionally to the various chemical compounds available within the medical markets.</p>",
"DOI": "10.5455/jabet.2022.d106",
"views": 841,
"downloads": 65,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/02/178-1628892758-Figure1.jpg",
"caption": "Figure 1. Cytotoxicity assay of T. chebula extracts on J774.1A cells. Cell viability was determined by MTT method. Negative control was considered 100% viable. The values were expressed as mean ±S.D and were performed in triplicates (p<0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/02/178-1628892758-Figure2.jpg",
"caption": "Figure 2. Effect of T. chebula extracts on LPS stimulated nitric oxide production from J774.1A cells. C-: Negative control (absence of LPS stimulus); C+: positive control (LPS treatment); inhibitor: L-NAME, NG-nitro-L-arginine-methyl ester; NO, nitric oxide. All the tests were done in triplicates and expressed as percent ± SD. (*P < 0.05). A: Hexane treatment; B: Ethylacetate; C: Chloroform of T. chebula extracts.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/02/178-1628892758-Figure3.jpg",
"caption": "Figure 3. Graph showing the effects of the extracts on LPS stimulated cytokine levels. Cells are treated with 6.25-100μg/mL of the chloroform and hexane extracts on stimulus with LPS for 24hr. A: IL-1β; B: IL-6 cytokine levels. Values are expressed as pg/ml ± SD. All the values were average of triplicates (P < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/02/178-1628892758-Figure4.jpg",
"caption": "Figure 4. Real time curves for the GAPDH, TNF- α and COX-2. A: GAPDH; B: TNF- α and COX-2. Ct values are average of duplicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/02/178-1628892758-Figure5.jpg",
"caption": "Figure 5. Graph showing the expression levels of GAPDH, TNF-α and COX-2 on treatment with the chloroform extracts. Negative control was without stimulus and not shown in the graph. GAPDH was used as calibrator. Its expression was considered as 100%. (P<0.005).",
"featured": false
}
],
"authors": [
{
"id": 98,
"affiliation": [
{
"affiliation": "Al-Qasim Green University, College of Biotechnology, Babylon Province, Iraq"
}
],
"first_name": "Haider Turky Mousa",
"family_name": "Al-Mousawi",
"email": "almusawi@biotech.uoqasim.edu.iq",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Haider Turky Mousa Al-Mousawi, Al-Qasim Green University, College of\r\nBiotechnology, Babylon Province, Iraq, e-mail: \r\nalmusawi@biotech.uoqasim.edu.iq",
"article": 39
},
{
"id": 99,
"affiliation": [
{
"affiliation": "Al-Qasim Green University, College of Biotechnology, Babylon Province, Iraq"
}
],
"first_name": "Al-Bderee Nadhim",
"family_name": "Mushtaq",
"email": "naz1988@biotech.uoqasim.edu.iq",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Al-Bderee Nadhim Mushtaq, Al-Qasim Green University, College of\r\nBiotechnology, Babylon Province, Iraq, e-mail: naz1988@biotech.uoqasim.edu.iq",
"article": 39
},
{
"id": 100,
"affiliation": [
{
"affiliation": "Diabetic national centre, Mustansiriyah University, Iraq"
}
],
"first_name": "Alyaa Hasan",
"family_name": "Bohan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 39
}
]
},
{
"id": 37,
"slug": "178-1628917330-impact-of-process-validation-and-equipment-qualification-in-production-of-bio-therapeutics",
"type": "review_article",
"manuscript_id": "178-1628917330",
"published": "2021-11-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/34/178-1628917330.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 163-175.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Ghosh G, Bodroth RP, Dutta B. Impact of process validation and equipment qualification in production of bio-therapeutics.J Adv Biotechnol Exp Ther. 2022; 5(1): 163-175.",
"title": "Impact of process validation and equipment qualification in production of bio-therapeutics",
"abstract": "<p>Production of therapeutic proteins like Biosimilars uses a complex cell based fermentation and purification process. There are chances of errors in these complex production processes due to complexities in the molecular pathway that every protein follows. Therefore to produce a desired therapeutic protein that elicit the correct immunological response in the patient, one need to ensure that it must have correct sequence, size, structure so that they are recognized by the specific receptors. The quality of these therapeutic proteins is characterized through standard assays and methods such as SDS PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis), IEF (Isoelectric focusing), Western Blot and other methods those are based on HPLC, Mass spectrometry and capillary electrophoresis to conform the desired molecular size, purity and identity of proteins. Thus in the process of validation of a method, equipment plays a critical role in ensuring the quality and safety of a biopharmaceutical product. It is always necessary to generate scientific data and information such as IQ (Installation qualification), OQ (Operational qualification) and PQ (Performance qualification) protocol and reports for each instrument employed in the process at the beginning and before the actual testing of the desired product. It is only after the completion of these activities, the product under different manufacturing steps can be characterized. Involvement of a software in conjunction to an equipment is also common and it is important that the software is in use with the equipment also meets the requirements of the CFR 21 FDA (Code of Federal Regulations, 21 Part 11 of US Food and Drug Administration), which is a legal requirement in a modern manufacturing process and hence it is important to comply with these rules as set of guidelines that will help to ensure the integrity and safety of data.</p>",
"DOI": "10.5455/jabet.2022.d105",
"views": 958,
"downloads": 540,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/43/02/178-1628917330-Figure1.jpg",
"caption": "Figure 1. Process validation flowchart.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/43/02/178-1628917330-Figure2.jpg",
"caption": "Figure 2. Equipment qualification flowchart.",
"featured": false
}
],
"authors": [
{
"id": 93,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Gandhi Institute of Engineering and Technology University, Gunupur - 765002, Odisha, India"
}
],
"first_name": "Goutam",
"family_name": "Ghosh",
"email": "goutamghosh1@rediffmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-8887-3310",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Goutam Ghosh, Professor; Department of Biotechnology, Gandhi Institute of Engineering and Technology University, Gunupur -765002, Odisha, India\r\nPhone: 9971019164, email: goutamghosh1@rediffmail.com",
"article": 37
},
{
"id": 94,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Gandhi Institute of Engineering and Technology University, Gunupur- 765002, Odisha, India"
}
],
"first_name": "Rabi Prasad",
"family_name": "Bodroth",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-0556-2494",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 37
},
{
"id": 95,
"affiliation": [
{
"affiliation": "Drug Regulatory Affairs Div, Panacea Biotec Limited, New Delhi, India"
}
],
"first_name": "Basabjit",
"family_name": "Dutta",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-4240-2732",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 37
}
]
},
{
"id": 36,
"slug": "178-1628422274-characterization-of-virulence-gene-distribution-and-antibiotic-susceptibility-profiles-of-diarrheagenic-escherichia-coli-from-chicken-faeces",
"type": "original_article",
"manuscript_id": "178-1628422274",
"published": "2021-10-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/57/178-1628422274.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 148-162.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Giri S, Kumar SH, K GS, et al. Characterization of virulence gene distribution and antibiotic susceptibility profiles of diarrheagenic Escherichia coli from chicken faeces.J Adv Biotechnol Exp Ther. 2022; 5(1): 148-162.'",
"title": "Characterization of virulence gene distribution and antibiotic susceptibility profiles of diarrheagenic Escherichia coli from chicken faeces",
"abstract": "<p>The inappropriate use of antibiotics in the poultry industry for prophylaxis and growth promotion has contributed to the development and spread of antibiotic resistance in zoonotic pathogens. This study aimed to understand the prevalence of pathogenic <em>Escherichia coli </em>(<em>E. coli</em>) in chicken faeces and their antibiotic susceptibility profiles. Forty-five <em>E. coli</em> isolated from the chicken faeces were further tested for virulence genes associated with diarrheagenic <em>E. coli </em>and extended-spectrum β-lactamase (ESBL) genes <em>bla</em><sub>CTX</sub>, <em>bla</em><sub>TEM</sub>, <em>bla</em><sub>SHV</sub> and Metallo-beta-lactamase gene <em>bla</em><sub>NDM</sub>. Results showed that <em>eaeA</em> and <em>astA</em> genes were detected in 60% of the isolates, followed by <em>bfpA </em>in 57.8%, <em>lt</em> in 26.7% and <em>st </em>gene in 2.2% of the isolates. Further, 15.5% of the isolates produced ESBL phenotypically whereas genotypically, one isolate was positive for <em>bla</em><sub>CTX</sub>, one for <em>bla</em><sub>TEM</sub>, and one for the <em>bla</em><sub>SHV </sub>gene. Twelve isolates (26.7%) harbored gene <em>bla</em><sub>NDM</sub>. High levels of resistance were observed against cefepime (93.3%), ertapenem (78.9%), meropenem (73.6%), tetracycline (88.9%), and cotrimoxazole (75.6%). This study highlights the importance of poultry as a source of human pathogenic multidrug-resistant (MDR) <em>E. coli </em>and their environmental dissemination.</p>",
"DOI": "10.5455/jabet.2022.d104",
"views": 1072,
"downloads": 143,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/02/178-1628422274-Figure1.jpg",
"caption": "Figure 1. A) ESBL detection by phenotypic confirmatory disk diffusion test. CAZ: ceftazidime; CAC: ceftazidime + clavulanic acid. B) Triple ESBL detection by E-strip method. C) MBL detection by E-strip method.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/02/178-1628422274-Figure2.jpg",
"caption": "Figure 2. Graph showing the antibiotic resistance pattern towards a set of antibiotics used for Escherichia coli isolates from chicken faces. Resistance percentage is mentioned above each antibiotic.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/02/178-1628422274-Figure3.jpg",
"caption": "Figure 3. PCR images for ESBL genes. (a) blaCTX gene. Lane M: 100 bp DNA ladder; lane 1: positive control; lane 2: negative control; lane 3 and 4: Escherichia coli isolates. (b) blaSHV gene. Lane M: 100 bp DNA ladder; lane 1: positive control; lane 2: negative control; lane 3 and 4: Escherichia coli isolates. (c) blaTEM gene. Lane M: 100 bp DNA ladder; lane 1: negative control; lane 2: positive control; lane 3,4 and 5: Escherichia coli isolates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/55/02/178-1628422274-Figure4.jpg",
"caption": "Figure 4. PCR assay for DEC pathotypes. (a) astA gene of EAEC. Lane M: 100 bp DNA ladder; lane PC: positive control; lane NC: negative control; lane 1, 2 and 3: positive isolates; lane 4: negative isolate. (b) bfpA gene of EPEC. Lane M: 100 bp DNA ladder; lane PC: positive control; lane 1, 3, 4 and 5: positive isolates; lane 2 and 6: negative isolates. (c) lt gene of ETEC. Lane M: 100 bp DNA ladder; lane NC: negative control; lane 2, 3 and 4: positive isolates; lane 1: negative isolate. (d) eaeA gene of EPEC. Lane M: 100 bp DNA ladder; lane PC: positive control; lane NC: negative control; lanes 1, 2, 3 and 4: positive isolates; lane 5: negative isolate. (e) st gene of ETEC. Lane M: 100 bp DNA ladder; lane NC: negative control; lane 1: positive isolate; lane 2: negative isolate. (f) blaNDM gene for metallo-beta-lactamase. Lane M: 100 bp DNA ladder; lane PC: positive control; lane NC: negative control; lane 1 and 2: positive isolates; lane 3: negative isolate.",
"featured": false
}
],
"authors": [
{
"id": 87,
"affiliation": [
{
"affiliation": "KS Hegde Medical Academy, Nitte (Deemed to be University), Mangalore- 575018, India"
}
],
"first_name": "Shobha",
"family_name": "Giri",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-8098-4868",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 36
},
{
"id": 89,
"affiliation": [
{
"affiliation": "Post-Harvest Technology, ICAR-Central Institute of Fisheries Education (CIFE), Versova, Andheri (W), Mumbai- 400061, India"
}
],
"first_name": "Sanath H.",
"family_name": "Kumar",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-0223-9069",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 36
},
{
"id": 90,
"affiliation": [
{
"affiliation": "College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangalore- 575002, India"
}
],
"first_name": "Girisha S.",
"family_name": "K",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 36
},
{
"id": 91,
"affiliation": [
{
"affiliation": "KS Hegde Medical Academy, Nitte (Deemed to be University), Mangalore- 575018, India"
}
],
"first_name": "A. Veena",
"family_name": "Shetty",
"email": "veenashetty@nitte.edu.in",
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-9522-1185",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "A. Veena Shetty, PhD; Department of Microbiology, KS Hegde Medical Academy, Nitte (Deemed to be University), Mangalore- 575018, India.\r\ne-mail: veenashetty@nitte.edu.in",
"article": 36
}
]
},
{
"id": 33,
"slug": "178-1627910384-analysis-of-floristic-diversity-of-the-forest-ecosystems-of-the-zat-valley-high-atlas-of-morocco-valorization-and-conservation-perspectives",
"type": "original_article",
"manuscript_id": "178-1627910384",
"published": "2021-10-03",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/13/178-1627910384.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 126-135.",
"academic_editor": "Md. Niamul Haque, PhD; Incheon National University, South Korea",
"cite_info": "Mostakim L, Guennoun FZ, Fetnassi N , et al. Analysis of floristic diversity of the forest ecosystems of the Zat valley- High Atlas of Morocco: Valorization and Conservation perspectives. J Adv Biotechnol Exp Ther. 2022; 5(1): 126-135.",
"title": "Analysis of floristic diversity of the forest ecosystems of the Zat valley- High Atlas of Morocco: Valorization and Conservation perspectives",
"abstract": "<p>From a management and conservation perspective, measuring the biodiversity of an ecosystem is an indispensable tool for assessing its health. Currently, scientists and authorities are giving great importance to the Zat valley because of its ecological, socio-economic and cultural importance. This study was carried out to assess the floristic biodiversity and to determine the different dominant plant groups in the Zat Valley. Based on 33 phyto-ecological surveys, the floristic diversity of the Zat valley revealed the existence of 114 species and subspecies distributed among 44 families and 96 genera, belonging to various biological types. As a first result, 9 taxa were reported as rare taxa according to the flora of Morocco, whereas 7 species were reported as rare and threatened species. A Correspondence Factor Analysis was performed in this study and allowed the individualization of five groups of plants: Pine formation, Thuja formation, Tamarix formation, Holm Oaks formation and Thuriferous formation, each of these formations was described by its geographical distribution, the biodiversity of its flora and its ecological conditions. This study provides an essential tool for the sustainable management of this valley through the implementation of a management and conservation plan.</p>",
"DOI": "10.5455/jabet.2022.d102",
"views": 1138,
"downloads": 264,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/02/178-1627910384-Figure1.jpg",
"caption": "Figure 1. Geographical location of prospected stations in the Zat Valley.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/02/178-1627910384-Figure2.jpg",
"caption": "Figure 2. Biological types and relative proportion of vascular plants recorded in the Zat Valley.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/02/178-1627910384-Figure3.jpg",
"caption": "Figure 3. Ordination plots of CFA illustrating the relationship between environmental factors and surveys prospected. Abbreviations (Alt: Altitude; GR%: General recovering%; RS: Richness species).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/02/178-1627910384-Figure4.jpg",
"caption": "Figure 4. Different plant formations recorded in the Zat valley (a. Aleppo pine formation; b. Riparian vegetation; c. Holm oaks formation; d. Thuriferous formation).",
"featured": false
}
],
"authors": [
{
"id": 74,
"affiliation": [
{
"affiliation": "Research Center of the Museum of Natural History, Cadi Ayyad University, Marrakesh, Morocco"
},
{
"affiliation": "Laboratory of water, biodiversity and climate change, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco."
}
],
"first_name": "Lahcen",
"family_name": "Mostakim",
"email": "mostakim.lahcen@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Lahcen Mostakim, \r\nResearch Center of the Museum of Natural History, Cadi Ayyad\r\nUniversity, Marrakesh, Morocco\r\nPhone: +212-698-94-7320",
"article": 33
},
{
"id": 75,
"affiliation": [
{
"affiliation": "Research Center of the Museum of Natural History, Cadi Ayyad University, Marrakesh, Morocco"
},
{
"affiliation": "Laboratory of water, biodiversity and climate change, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco."
}
],
"first_name": "Fatima Zahra",
"family_name": "Guennoun",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 33
},
{
"id": 76,
"affiliation": [
{
"affiliation": "Research Center of the Museum of Natural History, Cadi Ayyad University, Marrakesh, Morocco"
},
{
"affiliation": "Laboratory of water, biodiversity and climate change, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco."
}
],
"first_name": "Nidal",
"family_name": "Fetnassi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 33
},
{
"id": 77,
"affiliation": [
{
"affiliation": "Research Center of the Museum of Natural History, Cadi Ayyad University, Marrakesh, Morocco"
},
{
"affiliation": "Laboratory of water, biodiversity and climate change, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco."
}
],
"first_name": "Mohamed",
"family_name": "Ghamizi",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 33
}
]
},
{
"id": 31,
"slug": "178-1631157254-multidisciplinary-approaches-to-coping-with-neurodegenerative-disorders-amid-covid-19-pandemic",
"type": "review_article",
"manuscript_id": "178-1631157254",
"published": "2021-10-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/43/178-1631157254.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 100-114.",
"academic_editor": "Md Nabiul Islam, PhD\r\nYamaguchi University, Japan",
"cite_info": "Hannan MA, Dash R, Timalsina B, et al. Multidisciplinary approaches to coping with neurodegenerative disorders amid COVID-19 pandemic. J Adv Biotechnol Exp Ther. 2022; 5(1): 100-114.",
"title": "Multidisciplinary approaches to coping with neurodegenerative disorders amid COVID-19 pandemic",
"abstract": "<p>Neurodegenerative disorders, including Alzheimer’s and Parkinson’s, are the leading causes of dementia in the elderly. In the coming days, an alarming upsurge of dementia patients is expected with increasing life expectancy. This is the scenario not only in the developed world but also in the developing world, where older people live in vulnerable situations. Even in the COVID-19 (coronavirus disease-19) pandemic, the situation has worsened. Due to the limitations of conventional therapeutic strategies, it is necessary to explore integrated approaches consisting of both pharmacological and non-pharmaceutical interventions. As existing anti-dementia drugs pose many adverse effects on patients, pharmacological intervention through naturally occurring agents should be employed to explore targeted therapy. Alongside, non-pharmacological interventions such as cognitive and motor rehabilitation, occupational therapy, and psychological therapy need to be explored. From this perspective, multidisciplinary approaches need to be employed in order to develop a sustainable patient-friendly treatment strategy for the management of these emerging health issues with tremendous social burdens.</p>",
"DOI": "10.5455/jabet.2022.d100",
"views": 1028,
"downloads": 126,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/02/178-1631157254-Figure1.jpg",
"caption": "Figure 1. An outline on the multidisciplinary approaches in the management of neurodegenerative disorders.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/17/02/178-1631157254-Figure2.jpg",
"caption": "Figure 2. Pharmacological interventions through phytochemicals as a potential approach against neurodegenerative disorders. Phytochemicals can activate Nrf2 pathway and thereby induce antioxidant defense system that protects against oxidative stress-induced cellular damage. Phytochemicals can also activate protein clearance system and thereby protect neurons from degeneration. KEAP1, Kelch-like ECH-associated protein 1; Nrf2, Nuclear factor erythroid 2-related factor 2; GPx, Glutathione peroxidase; SOD, Superoxide dismutase; HO-1, Heme oxygenase-1; GSH, Glutathione; ROS, Reactive oxygen species.",
"featured": false
}
],
"authors": [
{
"id": 61,
"affiliation": [
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea"
},
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Abdul",
"family_name": "Hannan",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-6640-4495",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 31
},
{
"id": 63,
"affiliation": [
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea"
}
],
"first_name": "Raju",
"family_name": "Dash",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-4128-5308",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 31
},
{
"id": 64,
"affiliation": [
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea"
}
],
"first_name": "Binod",
"family_name": "Timalsina",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 31
},
{
"id": 65,
"affiliation": [
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea"
}
],
"first_name": "Yeasmin Akter",
"family_name": "Munni",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 31
},
{
"id": 66,
"affiliation": [
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea"
}
],
"first_name": "Mitra",
"family_name": "Sarmistha",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 31
},
{
"id": 67,
"affiliation": [
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea"
}
],
"first_name": "Diyah Fatimah",
"family_name": "Oktaviani",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 31
},
{
"id": 68,
"affiliation": [
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea"
},
{
"affiliation": "Department of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Patuakhali-8602, Bangladesh"
}
],
"first_name": "Md. Nazmul",
"family_name": "Haque",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 31
},
{
"id": 69,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Abdullah Al Mamun",
"family_name": "Sohag",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 31
},
{
"id": 70,
"affiliation": [
{
"affiliation": "Faculty of Fisheries and Marine Science, Center for Maritime Biosciences Studies, Jenderal Soedirman University, Purwokerto 53123, Indonesia"
}
],
"first_name": "Maria Dyah Nur",
"family_name": "Meinita",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 31
},
{
"id": 62,
"affiliation": [
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea"
}
],
"first_name": "Il Soo",
"family_name": "Moon",
"email": "moonis@dongguk.ac.kr",
"author_order": 10,
"ORCID": "https://orcid.org/0000-0002-1608-016X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Il Soo Moon, PhD; Department of Anatomy, Dongguk University, College of\r\nMedicine, Gyeongju 38066, Republic of Korea, e-mail: moonis@dongguk.ac.kr",
"article": 31
}
]
},
{
"id": 32,
"slug": "178-1628834342-epidemiological-comparison-of-the-first-and-second-wave-of-covid-19-pandemic-in-dhaka-bangladesh-a-cross-sectional-study-among-suspected-cases",
"type": "original_article",
"manuscript_id": "178-1628834342",
"published": "2021-10-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/07/178-1628834342.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 115-125.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Islam MM, Israk MF, Jahan MS. Epidemiological comparison of the first and second wave of COVID-19 pandemic in Dhaka, Bangladesh: A cross-sectional study among suspected cases. J Adv Biotechnol Exp Ther. 2022; 5(1): 115-125.",
"title": "Epidemiological comparison of the first and second wave of COVID-19 pandemic in Dhaka, Bangladesh: A cross-sectional study among suspected cases",
"abstract": "<p>Many countries have been seen two wave patterns of reported cases of coronavirus diseases. Still, a limited number of studies compared the epidemiological attributes of the first wave and second wave of coronavirus diseases 2019 (COVID-19) outbreak. This study was carried out to compare the epidemics of two waves in Bangladesh. This observational study data of suspected cases was collected from June 2020 to April 2021 from a local authorized diagnostic center. From June 27, 2020, to July 27, 2020, was considered as the first wave and from March 20, 2021, to April 20, 2021, was considered a second wave. This period was determined because of observing the highest number of positive cases during that period. Out of 21,359 cases, including 2429 (11.37%) individuals in the first wave and 4989 (23.36%) individuals in the second wave had RT-PCR test for disease confirmation. Among them, 315 people in the first wave and 630 in the second wave were positive for SARS-CoV-2 RNA. Male were more vernalized to the positive case, including 204 (64.8%) and 421 (66.8%) during the first and second waves, respectively. People under 41 found 56.2% positive cases during the first wave while it was 65.6% in the second wave. We also found 128 positive cases among international travelers. In conclusion, a large extent of different epidemiological attributes was found in the second wave compared to the first wave of the outbreaks.</p>",
"DOI": "10.5455/jabet.2022.d101",
"views": 1455,
"downloads": 150,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/02/178-1628834342-Figure1.jpg",
"caption": "Figure 1. Severity of the infection rate during second wave compared to the first wave among our collected data.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/02/178-1628834342-Figure2.jpg",
"caption": "Figure 2. Distribution by age intervals of the positive cases of Covid-19 during the first wave and second wave.",
"featured": false
}
],
"authors": [
{
"id": 71,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalgonj8100, Bangladesh"
}
],
"first_name": "Md. Mirajul",
"family_name": "Islam",
"email": "mirajulbge033@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-1475-8034",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Mirajul Islam; Department of Biotechnology and Genetic Engineering\r\nBangabandhu Sheikh Mujibur Rahman Science and Technology\r\nUniversity, Gopalgonj-8100, Bangladesh. e-mail: mirajulbge033@gmail.com",
"article": 32
},
{
"id": 72,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalgonj8100, Bangladesh."
}
],
"first_name": "Md. Fahim",
"family_name": "Ishrak",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 32
},
{
"id": 73,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Gono Bishwabidyalay, Dhaka-1344, Bangladesh."
}
],
"first_name": "Md. Shoykot",
"family_name": "Jahan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 32
}
]
},
{
"id": 34,
"slug": "178-1631542818-morphology-diversity-and-phylogenetic-analysis-of-spodoptera-exigua-lepidoptera-noctuidae-in-north-sulawesi-by-employing-partial-mitochondrial-cytochrome-oxidase-1-gene-sequences",
"type": "original_article",
"manuscript_id": "178-1631542818",
"published": "2021-09-09",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/12/178-1631542818.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2022; 5(1): 136-147.",
"academic_editor": "Shahed Uddin Ahmed Shazib, PhD; Smith College, USA.",
"cite_info": "Satiman U, Tulung M, Pelealu J, et al. Morphology, diversity and phylogenetic analysis of Spodoptera exigua (Lepidoptera: Noctuidae) in North Sulawesi by employing partial mitochondrial cytochrome oxidase 1 gene sequences. J Adv Biotechnol Exp Ther. 2022; 5(1): 136-147.",
"title": "Morphology, diversity and phylogenetic analysis of Spodoptera exigua (Lepidoptera: Noctuidae) in North Sulawesi by employing partial mitochondrial cytochrome oxidase 1 gene sequences",
"abstract": "<p><em>Spodoptera exigua</em> (Hübner, 1808) (Lepidoptera: Noctuidae) is a significant agricultural crop pest in Indonesia, causing significant economic losses in recent years. This species’ ability to survive on a wide variety of host plants provides an adaptive advantage for survival in the environment, which is facilitated by its high mobility, fecundity, and capability to acquire resistance to a broad spectrum of chemical pesticides. It is well-established that knowledge of diversity and evolutionary origins facilitate the development of pest management strategies. In the present study, we report the morphology, diversity and phylogeny analysis of <em>S. exigua</em> from North Sulawesi, Indonesia. The specimen from Rurukan have a body size and other segments that are longer than in Langowan and Modoinding. Dendrogram analysis shows that the similarity distance based on morphology ranges from 1-25%, which forms four clusters, where the specimen from Rurukan is separated from the rest of the specimens. The phylogeny of <em>S. exigua</em> from North Sulawesi, Indonesia, based on CO1 (Cytochrome c oxidase subunit 1) gene fragment, which is juxtaposed with CO1 data of the allied species from many geographical locations. A total of twenty-five isolates representing Indonesia, Japan, Germany, Thailand, India, UK, USA, Spain and Australia were compared. Nineteen sequences of <em>S. exigua</em> retrieved from GenBank were selected as references based on previous published phylogenic trees. The twenty-four isolates were scattered in two distinct clades indicating <em>S. exigua</em> is polyphyletic, but <em>S. exigua</em> from North Sulawesi, Indonesia is monophyletic.</p>",
"DOI": "10.5455/jabet.2022.d103",
"views": 1198,
"downloads": 161,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/03/178-1631542818-Figure1.jpg",
"caption": "Figure 1. Sampling location.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/59/03/178-1631542818-Figure2.jpg",
"caption": "Figure 2. Dendrogram of morphological character grouping of S. exigua sampled from Rurukan, Langowan, and Modoinding.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/44/04/178-1631542818-Figure3.jpg",
"caption": "Figure 3. Molecular phylogenetic analysis inferred by using Maximum Likelihood method based on Kimura 2-parameter (19). The percentage of trees with the relevant taxa clustered together is presented alongside the branches.",
"featured": false
}
],
"authors": [
{
"id": 78,
"affiliation": [
{
"affiliation": "Entomology Study Program, Postgraduate Program, Sam Ratulangi University, Manado, 95115, North Sulawesi, Indonesia."
}
],
"first_name": "Utari",
"family_name": "Satiman",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 34
},
{
"id": 79,
"affiliation": [
{
"affiliation": "Entomology Study Program, Postgraduate Program, Sam Ratulangi University, Manado, 95115, North Sulawesi, Indonesia."
}
],
"first_name": "Max",
"family_name": "Tulung",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 34
},
{
"id": 80,
"affiliation": [
{
"affiliation": "Entomology Study Program, Postgraduate Program, Sam Ratulangi University, Manado, 95115, North Sulawesi, Indonesia."
}
],
"first_name": "Jantje",
"family_name": "Pelealu",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 34
},
{
"id": 81,
"affiliation": [
{
"affiliation": "Entomology Study Program, Postgraduate Program, Sam Ratulangi University, Manado, 95115, North Sulawesi, Indonesia."
}
],
"first_name": "Christina Leta",
"family_name": "Salaki",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 34
},
{
"id": 82,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado-95115, North Sulawesi, Indonesia"
}
],
"first_name": "Beivy Jonathan",
"family_name": "Kolondam",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 34
},
{
"id": 83,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado-95115, North Sulawesi, Indonesia"
}
],
"first_name": "Trina Ekawati",
"family_name": "Tallei",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 34
},
{
"id": 84,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh"
}
],
"first_name": "Talha Bin",
"family_name": "Emran",
"email": "talhabmb@bgctub.ac.bd",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0003-3188-2272",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran, PhD; Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh\r\ne-mail: talhabmb@bgctub.ac.bd",
"article": 34
},
{
"id": 85,
"affiliation": [
{
"affiliation": "Department of Agrotechnology, Faculty of Agriculture Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Arthur",
"family_name": "Pinaria",
"email": "arthur.pinaria@unsrat.ac.id",
"author_order": 8,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Arthur Pinaria, PhD; Department of Agrotechnology, Faculty of Agriculture Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia. e-mail: arthur.pinaria@unsrat.ac.id",
"article": 34
}
]
}
]
}
],
"title": "2022",
"slug": "2022",
"order": 5
},
{
"id": 2,
"issues": [
{
"id": 15,
"title": "Vol4 Issue3",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2021",
"slug": "2021-vol4-issue3",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 237,
"slug": "178-1629358490-investigation-of-growth-performance-lipid-profile-and-liver-histotexure-of-mice-treated-with-butyric-acid",
"type": "original_article",
"manuscript_id": "178-1629358490",
"published": "2021-09-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/46/178-1629358490.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 415-423.",
"academic_editor": "Md Jamal Uddin, PhD Ewha Womans University,Seoul, South Korea.",
"cite_info": "Akter MT, Bhuiyan MEJ, et al. Investigation of growth performance, lipid profile, and liver histotexure of mice treated with butyric acid. J Adv Biotechnol Exp Ther. 2021; 4(3): 415-423.",
"title": "Investigation of growth performance, lipid profile, and liver histotexure of mice treated with butyric acid",
"abstract": "<p>Obesity is a pandemic which adds greatly to health care costs through its association with type 2 diabetes, metabolic syndrome, cardiovascular disease, and cancers. The aim of this research is to investigate the effects of different concentrations of butyric acid on changes of body weight, blood biochemical parameters, and liver histotexture in mice. Total twenty Swiss albino mice were divided into 4 groups viz Group A, B, C and D. Group A act as control while Group B, Group C and Group D were received 3, 5, and 7 mM/kg body weight butyric acid respectively, throughout the experimental period. On the 60th day, blood and liver samples were collected for biochemical analysis and histopathology respectively. Results showed that body weight increased significantly (p<0.05) in Group C and D compared to control. The total cholesterol (TC) and triglyceride (TG) were significantly higher in Group B (p<0.05) and D (p<0.01) compared to the control group. The high-density lipoprotein (HDL) was increased significantly (p<0.05) in Group D whereas no significant changes were found in low density lipoprotein (LDL) level compared to control. The serum glucose was significantly higher in Group B, C (p<0.05) and D(p<0.01) than control group. In histopathological studies, liver of group B showed slight hepatocellular degeneration whereas group C and D showed vacuolation of hepatocytes. From the above findings it can be concluded that higher concentration of butyric acid may cause rise in body weight, the lipid profile and degenerative hepatocellular changes in mice, whereas lower concentration did not make significant changes.</p>",
"DOI": "10.5455/jabet.2021.d140",
"views": 1353,
"downloads": 172,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/00/06/178-1629358490-Figure1.jpg",
"caption": "Figure 1. Effects of butyric acid on body weight gain in mice (mean ± SD). Group A is control group; Group B was treated with 3 mM/kg bw butyric acid; Group C was treated with 5 mM/kg bw butyric acid; group D was treated with 7 mM/kg bw butyric acid. *P < 0.05 (group A vs. group C and group A vs. group D).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/00/06/178-1629358490-Figure2.jpg",
"caption": "Figure 2. Effects of butyric acid on lipid profile (TC, TG, HDL and LDL) in mice (mean ± SD). Group A is control group; Group B was treated with 3 mM/kg bw butyric acid; Group C was treated with 5 mM/kg bw butyric acid; group D was treated with 7 mM/kg bw butyric acid. *P < 0.05 (group A vs. group C and group A vs. group D). In case of effects of butyric acid on serum TC in mice, *P < 0.05 (group A vs. group B) and **P < 0.01 (group A vs. group D), in case of effects of butyric acid on TG in mice, *P < 0.05 (group A vs. group C and group A vs. group D), in case of effects of butyric acid on HDL in mice, *P < 0.05 (group A vs. group D).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/00/06/178-1629358490-Figure3.jpg",
"caption": "Figure 3. Effects of butyric acid on serum glucose level in mice (mean ± SD). Group A is control group; Group B was treated with 3 mM/kg bw butyric acid; Group C was treated with 5 mM/kg bw butyric acid; group D was treated with 7 mM/kg bw butyric acid. *P < 0.05 (group A vs. group C and group A vs. group D). where *P< 0.05 than control (group A vs. group B and group A vs. group C) and **P < 0.01 (group A vs. group D).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/00/06/178-1629358490-Figure4.jpg",
"caption": "Figure 4. Histopathological changes in liver of mice. Liver of each experimental group was collected and was processed for histopathological study. Photographs were taken under microscope (Labomed, Inc., Los Angeles, USA) at 400 X magnification. Group A is control group; Group B was treated with 3 mM/kg bw butyric acid; Group C was treated with 5 mM/kg bw butyric acid; group D was treated with 7 mM/kg bw butyric acid. Marked hepatocellular changes were observed in group D such as enlargement of central vein, degeneration of hepatocytes around the central vein and hepatocellular death.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/00/06/178-1629358490-Figure5.jpg",
"caption": "Figure 5. Graphical representation of abstract. Briefly, at first the adaptation period with normal diet, then treatment with different doses of butyric acid such as 3, 5 and 7 mM/kg bw whereas other than them one group is remained control. Secondly, sacrificed at day 70 and samples were collected for histopathology and biochemical analysis.",
"featured": false
}
],
"authors": [
{
"id": 1094,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Most. Tania",
"family_name": "Akter",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 237
},
{
"id": 1095,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Eftakhar Jahan",
"family_name": "Bhuiyan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 237
},
{
"id": 1096,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Khaled Mahmud",
"family_name": "Sujan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 237
},
{
"id": 1097,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Ziaul",
"family_name": "Haque",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 237
},
{
"id": 1098,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sharmin",
"family_name": "Akter",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 237
},
{
"id": 1099,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Golzar",
"family_name": "Hossain",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 237
},
{
"id": 1100,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Kamrul",
"family_name": "Islam",
"email": "k.physiol@bau.edu.bd",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Kamrul Islam, PhD Department of Physiology, Bangladesh Agricultural \r\nUniversity, Mymensingh-2202, Bangladesh. E-mail: k.physiol@bau.edu.bd",
"article": 237
}
]
},
{
"id": 234,
"slug": "178-1597824526-comparison-of-major-nutritional-constituents-and-genetic-diversity-analysis-of-five-strains-of-oyster-mushrooms",
"type": "original_article",
"manuscript_id": "178-1597824526",
"published": "2021-09-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/00/178-1597824526.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 405-414.",
"academic_editor": "Akhi Moni, PhD ABEx Bio-Research Center Bangladesh",
"cite_info": "Sultana A, Sarker NC, et al. Comparison of major nutritional constituents and genetic diversity analysis of five strains of oyster mushrooms. J Adv Biotechnol Exp Ther. 2021; 4(3): 405-414.",
"title": "Comparison of major nutritional constituents and genetic diversity analysis of five strains of oyster mushrooms",
"abstract": "<p>Oyster mushroom is the second runner-up among commercially produced mushrooms due to its delicious taste, higher nutritional and medicinal properties. The objectives of this study were to determine the nutritional variation through the analysis of obtained nutritional values and to assess the genetic diversity through RAPD marker of five different strains of oyster mushrooms namely <em>Pleurotus cystidiosus </em>(strain: pcys2)<em>; Pleurotus djamor </em>(pop1)<em>; Pleurotus ostreatus </em>(ws); <em>Pleurotus ostreatus </em>(po3) <em>and Pleurotus geesteranus </em>(pg4). The strains showed variations in moisture, protein, fiber, lipid, ash and carbohydrate content ranged from 86.10-87.33%; 17.8-24.13 gm/100gm; 18.16-25.46 gm/100gm; 3.16-5.16 gm/100gm; 9.16-11.46 gm/100gm; and 35.4-45.33 gm/100gm respectively. In case of genetic diversity, the segregation of five strains of oyster mushrooms were grouped through un-weighted pair group method of arithmetic means average (UPGMA), where strains were grouped into two main clusters and the generated linkage distance was 48. The strains pop1 and po3 were aligned in cluster two (C2) due to their genetic similarity but showed dissimilarities with other strains. Though the strains pcys2<em>, </em>ws, and pg4 were aligned in the same cluster (C1), the strain ws was aligned in a different sub-cluster due to its few dissimilarities with the other two strains. The variation of nutritional values and genetic diversities among the mushroom strains indicates nutritional and genetic variabilities. The findings of current study indicate that, though these mushrooms were genetically dissimilar, all strains were nutritious with high protein and fiber contents with low fat. However, mushroom breeder can consider strain po3 for high protein content, strain ws for high fiber content and strain pop1 for low fat content.</p>",
"DOI": "10.5455/jabet.2021.d139",
"views": 676,
"downloads": 118,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/06/178-1597824526-Figure1.jpg",
"caption": "Figure 1. RAPD profiles of primers generated from 5 different Oyster mushrooms through 1.4% agarose gel electrophoresis. Herein, at figure (A), (B) and (C): M1 and M2 denotes DNA ladder (Marker); Lane 1(S1) = P. cystidiosus (pcys2), Lane 2(S2) = P. djamor (pop1), Lane 3(S3) = P. ostreatus (ws), Lane 4(S4) = P. ostreatus (po3), Lane 5(S5) = P. geesteranus (pg4).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/06/178-1597824526-Figure2.jpg",
"caption": "Figure 2. Cluster analysis of five oyster mushrooms based on six RAPD markers by UPGMA.",
"featured": false
}
],
"authors": [
{
"id": 1078,
"affiliation": [
{
"affiliation": "Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University, Kushtia - 7003, Bangladesh"
}
],
"first_name": "Aklima",
"family_name": "Sultana",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 234
},
{
"id": 1079,
"affiliation": [
{
"affiliation": "National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh."
}
],
"first_name": "Nirod Chandra",
"family_name": "Sarker",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 234
},
{
"id": 1080,
"affiliation": [
{
"affiliation": "National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh."
}
],
"first_name": "Akhter Jahan",
"family_name": "Kakon",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 234
},
{
"id": 1081,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia - 7003, Bangladesh"
}
],
"first_name": "Shifath Bin",
"family_name": "Syed",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 234
},
{
"id": 1082,
"affiliation": [
{
"affiliation": "Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University, Kushtia - 7003, Bangladesh"
}
],
"first_name": "Md. Toufiq",
"family_name": "Elahi",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 234
},
{
"id": 1083,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia - 7003, Bangladesh"
}
],
"first_name": "Sudhangshu Kumar",
"family_name": "Biswas",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 234
},
{
"id": 1084,
"affiliation": [
{
"affiliation": "Division of Microbiology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia"
}
],
"first_name": "Swee-Seong",
"family_name": "Tang",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 234
},
{
"id": 1085,
"affiliation": [
{
"affiliation": "Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University, Kushtia - 7003, Bangladesh"
}
],
"first_name": "Anisur",
"family_name": "Rahman",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 234
},
{
"id": 1086,
"affiliation": [
{
"affiliation": "Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University, Kushtia - 7003, Bangladesh"
}
],
"first_name": "Dipak Kumar",
"family_name": "Paul",
"email": "dpaul@anft.iu.ac.bd",
"author_order": 9,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dipak Kumar Paul, PhD Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University, Kushtia - 7003, Bangladesh e-mail: dpaul@anft.iu.ac.bd",
"article": 234
}
]
},
{
"id": 233,
"slug": "178-1624968570-assessment-of-the-anti-leukemic-and-antioxidant-potential-of-the-methanol-extract-of-a-wild-edible-and-novel-mushroom-astraeus-hygrometricus-and-unraveling-its-metabolomic-profile",
"type": "original_article",
"manuscript_id": "178-1624968570",
"published": "2021-09-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/22/178-1624968570.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 388-404.",
"academic_editor": "Md Jamal Uddin, PhD Ewha Womans University, Seoul, South Korea",
"cite_info": "Pal A, Ray R, et al. Assessment of the anti-leukemic and antioxidant potential of the methanol extract of a wild, edible, and novel mushroom, Astraeus hygrometricus, and unraveling its metabolomic profile. J Adv Biotechnol Exp Ther. 2021; 4(3): 388-404.",
"title": "Assessment of the anti-leukemic and antioxidant potential of the methanol extract of a wild, edible, and novel mushroom, Astraeus hygrometricus, and unraveling its metabolomic profile",
"abstract": "<p>Mushrooms are enriched with a plethora of bioactive molecules that play a vital role in the prevention of human diseases. The balance between ROS generation and cancer growth is one of the main prerequisites for efficient cancer treatment. In this study, to testify the aforesaid theory, five wild edible mushrooms were initially screened based on their anti-proliferative efficiency, and the best mushroom extract was selected for the assessment of their antioxidant potentialities <em>in vitro </em>in various artificially generated free radicals such as DPPH, ABTS+, and by FRAP experiment. The reason behind the antiproliferative potentiality and antioxidant capability of the most potent extract was also correlated by profiling its metabolites through GC-MS analysis. The study reveals, that the methanolic extract of <em>Astraeus hygrometricus </em>is the most potent anti-leukemic extract (IC<sub>50 </sub>22.7 ±0.23 µg/mL) followed by <em>Serpula </em>sp. (75.7 ±0.44 µg/mL), <em>Phallus </em>sp. (60.53±0.36 µg/mL), <em>Tricholoma </em>sp<em>.</em> (53.76±0.46 µg/mL), <em>Lentinus </em>sp. (58 ±0.13 µg/mL) against the Jurkat cell line. The assessment of the antioxidant profile <em>Astraeus hygrometricus </em>reveals its moderate antioxidant efficacy against several artificially generated free radicals, such as DPPH (76.9±0.16 µg/mL), ABTS+ (142±0.66 µg/mL) and moderate iron chelating efficacy (32.37±2.31µM). The GC-MS analysis of both methanol and ethyl acetate extracts of <em>Astraeus hygrometricus </em>have found 53 and 52 compounds respectively, with wide diverse ranges of chemically classified biomolecules such as alkane, alcohol, fatty acid, organic acid, polycyclic and heterocyclic compounds, amino acid, vitamin, and hormone, etc. with a wide array of biological activity such as anticancer and antioxidant potentiality. In conclusion, it can be said that these wild edible mushroom <em>Astraeus hygrometricus </em>are a repository of novel biomolecules that can be used in the treatment of Leukemia in the future.</p>",
"DOI": "10.5455/jabet.2021.d138",
"views": 1548,
"downloads": 134,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/06/178-1624968570-Figure1.jpg",
"caption": "Figure 1. Habitat of the collected mushrooms morphology in its habitat: a. Astraeus hygrometricus (AH), b. Tricholoma sp(Tricho), c. Phallus sp(Pha), d. Serpula sp. (Ser), e .Lentinus sp. (Len). Selective anti-proliferative activity of ME (1f) and EA (1g) of five different mushrooms on Jurkat cell line (f,g).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/06/178-1624968570-Figure2.jpg",
"caption": "Figure 2. (a-d) Comparative study of antioxidant properties of the methanolic extract of five mushrooms Astraeus hygrometricus (AH), b. Tricholoma sp (Tricho), c. Phallus sp (Pha), and d. Serpula sp. (Ser) against DPPH free radical (2 a), ABTS+ free radical (2 b), Lipid peroxidation assay (2 d), and FRAP assay (2 c).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/06/178-1624968570-Figure3.jpg",
"caption": "Figure 3. (a-d) Metabolomic profiling of ethyl acetate and methanolic extract of Astraeus hygrometricus(AH). GC-MS chromatogram Ethyl acetate (EA) fraction of Astraeus hygrometricus (3a). GC-MS chromatogram Methanolic fraction of Astraeus hygrometricus(3b). Pie chart that has different sectors. Each sector represents a large group. Each extract of Astraeus hygrometricus has many compounds. Now compounds with similar chemical functional or structural structures are categorized under large groups. Each compound has its individual area percentage at a particular retention time. The area percentage of similar compounds in the particular large group represents the area percentage of a large group, this cumulative area percentage of each large group presents an angular sector in the pie chart. (3c) represents different large groups of compounds of ethyl acetate extract of Astraeus hygrometricus. There are 12 large groups present. (3d) represents different large groups of compounds of methanolic extract of Astraeus hygrometricus. There are 11 large groups present. (3e) Total Phenol content in respect to GAE/g of the methanolic extract of the collected mushrooms AH, Tricho, Pha, Ser, Len. (3f) Total Flavonoid content in respect to quercetin of the methanolic extract of AH, Tricho, Pha, Ser, Len.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/06/178-1624968570-Figure4.jpg",
"caption": "Figure 4. The two columns represent the compounds of ethyl acetate and methanolic extracts of Astraeus hygrometricus. The narrow column represents the gradual continuation shades of color black to white. It corresponds to a scale of 0-2 in Fig a, and 0-20 in Fig b. Here the scale denotes area percentage. Total compounds present in both the extract are compared on the basis of the area percentage of each compound. As maximum compounds are restricted to each extract but some are common to both extracts. Though they present in both extracts the common compounds differ in their area percentage. (a) Compounds of Astraeus hygrometricus both the extract having area percentage <2. (b) Compounds of Astraeus hygrometricus both the extract having area percentage > 2.",
"featured": false
}
],
"authors": [
{
"id": 1074,
"affiliation": [
{
"affiliation": "Laboratory of Cell and Molecular Biology, Department of Botany, University of Calcutta, Kolkata 700019, India"
}
],
"first_name": "Amrita",
"family_name": "Pal",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 233
},
{
"id": 1075,
"affiliation": [
{
"affiliation": "Laboratory of Cell and Molecular Biology, Department of Botany, University of Calcutta, Kolkata 700019, India"
}
],
"first_name": "Ribhu",
"family_name": "Ray",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 233
},
{
"id": 1076,
"affiliation": [
{
"affiliation": "Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata- 700019, India"
}
],
"first_name": "Krishnendu",
"family_name": "Acharya",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 233
},
{
"id": 1077,
"affiliation": [
{
"affiliation": "Laboratory of Cell and Molecular Biology, Department of Botany, University of Calcutta, Kolkata 700019, India"
}
],
"first_name": "Santanu",
"family_name": "Paul",
"email": "spaul_1971@yahoo.com",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Professor Santanu PaulLaboratory of Cell and Molecular Biology, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, \r\nKolkata 700019, West Bengal, India.e-mail: spaul_1971@yahoo.com",
"article": 233
}
]
},
{
"id": 232,
"slug": "178-1628068229-a-comprehensive-review-on-oilseed-cakes-and-their-potential-as-a-feedstock-for-integrated-biorefinery",
"type": "review_article",
"manuscript_id": "178-1628068229",
"published": "2021-08-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/21/178-1628068229.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 376-387.",
"academic_editor": "Akhi Moni, PhDABEx Bio-Research Center, Bangladesh.",
"cite_info": "Sarkar N, Chakraborty D, et al. A comprehensive review on oilseed cakes and their potential as a feedstock for integrated biorefinery. J Adv Biotechnol Exp Ther. 2021; 4(3): 376-387.",
"title": "A comprehensive review on oilseed cakes and their potential as a feedstock for integrated biorefinery",
"abstract": "<p>In oil processing industry, large number of oilseed cakes remains after the extraction of oil. Oilseed cakes and meals are the residues would act as valuable feeds for livestock as it is rich in protein. These agricultural residues serve as a major valuable feedstock for cattle and used for the production of value-added products such as biogas, biofuel, biopolymer, antibiotics, enzymes, etc. The usage of oilseed cake as raw material would reduce environmental pollution as well as the production cost. This can be served as a potential solution to overcome nutritional problems in animals by enriching the nutrition using appropriate technologies. As the world moving towards sustainable fuel and chemical synthesis using renewable resources deemed as second-generation biofuel feedstock rather than fossil fuels, these non-edible oilseed cakes are used to produce biofuels with an integrated biorefinery perspective. The oilseed cakes plays vital role in the area of enzyme and fermentation technology. The fermentation process using fungi could act as relatively low-cost appropriate technology for upgrading nutrients in oilseed cakes. Oilseed cakes are rich in fiber and have a high concentration of non-starch polysaccharides (NSP) such as cellulose and hemicellulose (Xylan). Lipase and alpha-amylase are the two most essential enzymes produces by the oilseed cake. This review focuses on exploiting the various oilseed cakes and their commercial utilization by implementing various biotechnological approaches.</p>",
"DOI": "10.5455/jabet.2021.d137",
"views": 4318,
"downloads": 250,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/06/178-1628068229-Figure1.jpg",
"caption": "Figure 1. Schematic diagram shows the oilseed cakes in biorefinery aspects.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/06/178-1628068229-Figure2.jpg",
"caption": "Figure 2. Flow-chart shows the processing of oilseeds and extraction of oil in the oil processing unit and usage of oilseed cake for ruminants feeding.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/06/178-1628068229-Figure3.jpg",
"caption": "Figure 3. Schematic diagram shows various kinds of protein rich oilseed cakes used as animal feed.",
"featured": false
}
],
"authors": [
{
"id": 1067,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur- 603203, Chengalpattu Dist., Tamil Nadu, India"
}
],
"first_name": "Nayanika",
"family_name": "Sarkar",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 232
},
{
"id": 1068,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur- 603203, Chengalpattu Dist., Tamil Nadu, India"
}
],
"first_name": "Debosmita",
"family_name": "Chakraborty",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 232
},
{
"id": 1069,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur- 603203, Chengalpattu Dist., Tamil Nadu, India"
}
],
"first_name": "Rishita",
"family_name": "Dutta",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 232
},
{
"id": 1070,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur- 603203, Chengalpattu Dist., Tamil Nadu, India"
}
],
"first_name": "Parul",
"family_name": "Agrahari",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 232
},
{
"id": 1071,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur- 603203, Chengalpattu Dist., Tamil Nadu, India"
}
],
"first_name": "Sundaram Deepika",
"family_name": "Bharathi",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 232
},
{
"id": 1072,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur- 603203, Chengalpattu Dist., Tamil Nadu, India"
}
],
"first_name": "Apurva Anand",
"family_name": "Singh",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 232
},
{
"id": 1073,
"affiliation": [
{
"affiliation": "Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur- 603203, Chengalpattu Dist., Tamil Nadu, India"
}
],
"first_name": "Samuel",
"family_name": "Jacob",
"email": "samueljb@srmist.edu.in",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Samuel Jacob Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur 603203, Chengalpattu Dist., Tamil Nadu, \r\nIndia.e-mail: samueljb@srmist.edu.in",
"article": 232
}
]
},
{
"id": 231,
"slug": "178-1624985691-functional-informativeness-of-lymphocytes-cytomorphometric-analysis-of-laboratory-rats-blood",
"type": "original_article",
"manuscript_id": "178-1624985691",
"published": "2021-08-09",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/30/178-1624985691.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 365-375.",
"academic_editor": "Md Jamal Uddin, PhD Ewha Womans University, Seoul, South Korea",
"cite_info": "Frolov OK, Lytvynenko RO, et al. Functional informativeness of lymphocytes’ cytomorphometric analysis of laboratory rats’ blood. J Adv Biotechnol Exp Ther. 2021; 4(3): 365-375.",
"title": "Functional informativeness of lymphocytes’ cytomorphometric analysis of laboratory rats’ blood",
"abstract": "<p>Immunological methods that objectively reflect the lymphogenetic processes in the examined organism are needed to assess the effectiveness of prophylactic or therapeutic agents that modulate regenerative processes in the immune system. Such requirements are met by the cytomorphometric method of analysis of circulating blood lymphocytes, the size (small, medium and large) populations of which reflect their proliferative processes in the lymphoid organs. However, the cytomorphometric characteristics of lymphocytes have species features that have to be determined experimentally. In the model of fecal peritonitis in white non-linear sexually mature male laboratory rats, the limits of statistical size classes of lymphocytes were determined, which were the same in control and experimental animals, namely: small – with a diameter of 8.5 μm and less, medium – with a diameter of more than 8.5 μm and less 11.0 μm, large – with a diameter of 11.0 μm and more. Acute infectious process significantly changed the levels of cytomorphometric classes of lymphocytes according to their functional activity in immunogenesis: small, medium and large size of lymphocytes as 42.5 to 7.0%, 45.0 to 54.0%, 10.5 to 30.0%, respectively, in the control and experimental groups of animals, which demonstrates the informativeness of the method. In this case, small and large lymphocytes belong to the activated lymphocytes, which determine the state of the immune system, while medium lymphocytes mainly make up the pool of memory cells. Based on the ratio of these size classes in the peripheral blood, a conclusion about the proliferative reaction of lymphocytes in the experiment is made, followed by the extrapolation of the results and in clinical practice.</p>",
"DOI": "10.5455/jabet.2021.d136",
"views": 1171,
"downloads": 133,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/00/06/178-1624985691-Figure1.jpg",
"caption": "Figure 1. Histogram of the blood lymphocytes' average diameter of sexually mature rats in normal (control, n=10) and pathological (fecal peritonitis, n=10) states. The results are presented as х ̅ ± m, where х ̅ is the average value of the attribute, m is the average error of the arithmetic mean. The arrows marked the dips by which we separated the small, medium and large cytomorphometric classes of lymphocytes.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/00/06/178-1624985691-Figure2.jpg",
"caption": "Figure 2. Morphology of small (A), medium (B, C) and large (D, E, F) lymphocytes in laboratory rats. Light microscope (objective lens 100×), Pappenheim staine. Scale bar: 10 μm.",
"featured": false
}
],
"authors": [
{
"id": 1064,
"affiliation": [
{
"affiliation": "Department of Physiology, Immunology and Biochemistry with the Course of Civil Defense and Medicine, Faculty of Biology, Zaporizhzhia National University, Zaporizhzhia, 69600, Ukraine."
}
],
"first_name": "Oleksandr Kyrylovych",
"family_name": "Frolov",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 231
},
{
"id": 1065,
"affiliation": [
{
"affiliation": "Department of Physiology, Immunology and Biochemistry with the Course of Civil Defense and Medicine, Faculty of Biology, Zaporizhzhia National University, Zaporizhzhia, 69600, Ukraine."
}
],
"first_name": "Raisa Oleksandrivna",
"family_name": "Lytvynenko",
"email": "r_litvinenko@ukr.net",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Raisa Oleksandrivna Lytvynenko Department of Physiology, Immunology and Biochemistry with the Course of Civil Defense and Medicine, Faculty of Biology, Zaporizhzhia National University, Zaporizhzhia, 69600, Ukraine. e-mail: r_litvinenko@ukr.net",
"article": 231
},
{
"id": 1066,
"affiliation": [
{
"affiliation": "Department of Histology, Cytology and Embryology, Medical Faculty No. 2, Zaporizhzhia State Medical University, Zaporizhzhia, 69035, Ukraine"
}
],
"first_name": "Lyudmyla Valeriyivna",
"family_name": "Makyeyeva",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 231
}
]
},
{
"id": 236,
"slug": "178-1624073760-antinociceptive-potential-of-methanol-leaf-extracts-of-cissampelos-parreira-linn-lantana-camara-linn-and-ocimum-gratissimum-african-basil",
"type": "original_article",
"manuscript_id": "178-1624073760",
"published": "2021-07-29",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/50/178-1624073760.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 349-364.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Kimuni SN, Gitahi SM, et al. Antinociceptive potential of methanol leaf extracts of Cissampelos parreira (Linn), Lantana camara (Linn) and Ocimum gratissimum (African basil). J Adv Biotechnol Exp Ther. 2021; 4(3): 349-364.",
"title": "Antinociceptive potential of methanol leaf extracts of Cissampelos parreira (Linn), Lantana camara (Linn) and Ocimum gratissimum (African basil)",
"abstract": "<p>The effects of <em>Cissampelos pareira </em>(Linn)<em>, Lantana camara </em>(Linn) and<em> Ocimum gratissimum</em> (African basil) leaf extracts on pain have not been biologically determined despite their frequent traditional use in pain management. The present study evaluated the effects of methanol leaf extracts of these three plants on formalin-induced pain in Swiss albino mice. Leaves of <em>C. pareira, L. camara,</em> and <em>O. gratissimum</em> were harvested, cleaned, shade dried, crushed, extracted in absolute methanol and concentrated to dryness. The quantitative phytochemical screening of the three plant extracts was first carried out. Then, the pain assay tests constituted eight groups of five mice each: normal control group, positive control group, negative control group and experimental groups of 50, 100, 150, 200 and 250 mg/kg bw extracts. The animals were administered with various treatments thirty minutes before induction of pain through injection of 0.01 ml of 2.5% formalin solution into the sub-plantar region of the left hind paw. Paracetamol at the dose of 50 mg/kg bw and 5% dimethyl sulfoxide were used as the positive and negative controls respectively. The plant extracts were administered intraperitoneally and orally. Data was analyzed using one-way analysis of variance and unpaired t-test. Phytochemical screening on separate extracts of <em>C. pareira, L. camara, </em>and <em>O. gratissimum</em> revealed fatty acids, phenols, flavonoids and terpenoids. The different dosages of methanol leave extracts of <em>C. pareira, L. camara, </em>and <em>O. gratissimum</em> reduced pain significantly (p˃0.05) in mice. The significant reduction of pain was associated with fatty acids, phenols, flavonoids and terpenoids revealed in the plant extracts.</p>",
"DOI": "10.5455/jabet.2021.d135",
"views": 1095,
"downloads": 173,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure1.jpg",
"caption": "Figure 1. Comparison between antinociceptive effects of oral and intraperitoneal administration of C. pareira in early phase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure2.jpg",
"caption": "Figure 2. Comparison between antinociceptive effects of oral and intraperitoneal administration of C. pareira in late phase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure3.jpg",
"caption": "Figure 3. Comparison between antinociceptive effects of oral and intraperitoneal administration of L. camara in late phase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure4.jpg",
"caption": "Figure 4. Comparison between antinociceptive effects of oral and intraperitoneal administration of L. camara in late phase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure5.jpg",
"caption": "Figure 5. Comparison between antinociceptive effects of oral and intraperitoneal administration of O. gratissimum in early phase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure6.jpg",
"caption": "Figure 6. Comparison between antinociceptive effects of oral and intraperitoneal administration of O. gratissimum in late phase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure7.jpg",
"caption": "Figure 7.\tComparison of antinociceptive effects of orally administrated C. pareira, L. camara and O. gratissimum extracts in the early phase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure8.jpg",
"caption": "Figure 8. Comparison of antinociceptive effects of orally administrated C. pareira, L. camara and O. gratissimum extracts in the late phase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure9.jpg",
"caption": "Figure 9. Comparison of antinociceptive effects of intraperitoneally administrated C. pareira, L. camara and O. gratissimum extracts in the early phase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/19/06/178-1624073760-Figure10.jpg",
"caption": "Figure 10. Comparison of antinociceptive effects of intraperitoneally administrated C. pareira, L. camara and O. gratissimum extracts in the late phase.",
"featured": false
}
],
"authors": [
{
"id": 1090,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Susan Nyawira",
"family_name": "Kimuni",
"email": "susankimuni@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Susan Nyawira Kimuni, PhD; Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya, e-mail: susankimuni@gmail.com",
"article": 236
},
{
"id": 1091,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Stephen Maina",
"family_name": "Gitahi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 236
},
{
"id": 1092,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Eliud Mwaniki",
"family_name": "Njagi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 236
},
{
"id": 1093,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya"
}
],
"first_name": "Mathew Piero",
"family_name": "Ngugi",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 236
}
]
},
{
"id": 235,
"slug": "178-1621847987-in-vivo-antidiabetic-efficacy-mineral-element-composition-and-qualitative-phytochemistry-of-the-aqueous-leaf-extracts-of-pentas-zanzibarica-klotzsch-vatke-and-olea-europaea-subspecies-africana-mill",
"type": "original_article",
"manuscript_id": "178-1621847987",
"published": "2021-07-18",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/36/178-1621847987.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 334-348.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, South Korea",
"cite_info": "Moriasi GA, Kibiti CM, et al. In vivo antidiabetic efficacy, mineral element composition, and qualitative phytochemistry of the aqueous leaf extracts of Pentas zanzibarica (Klotzsch.) Vatke and Olea europaea subspecies africana (Mill.).J Adv Biotechnol Exp Ther. 2021; 4(3): 334-348.",
"title": "In vivo antidiabetic efficacy, mineral element composition, and qualitative phytochemistry of the aqueous leaf extracts of Pentas zanzibarica (Klotzsch.) Vatke and Olea europaea subspecies africana (Mill.)",
"abstract": "<p>Persistent hyperglycemia is the hallmark of diabetes and is accountable for the devastating complications, which cause high morbidity and mortality. Conventional anti-diabetic agents are only palliative and characterized with limited efficacy, adverse effects, high costs, inaccessibility, prompting the need for better alternatives. Therefore, we investigated the <em>in vivo </em>hypoglycemic activities, elemental composition, and qualitative phytochemistry of the aqueous leaf extracts of <em>P. zanzibarica </em>and <em>O. europaea </em>subspecies <em>africana </em>as potential sources of affordable, safer, accessible, and potent anti-diabetic therapies. <em>In vivo </em>hypoglycemic activities of the studied plant extracts were evaluated at three dose levels of 50, 100, and 150 mg/kg body weight (bw) in Alloxan-induced hyperglycemic Swiss albino mice. The elemental composition of the plant extracts was analyzed using the energy dispersive x-ray fluorescence spectroscopy (EDXRF System) and the atomic absorption spectrometry (AAS). Qualitative phytochemical screening was done following standard procedures. In this study, the aqueous leaf extract of <em>P. zanzibarica</em>, significantly (p<0.05) reduced alloxan-induced hyperglycemia in mice from 163.26±2.24 mg/dL (at 0 h) to 52.12±1.16 mg/dL (at the 4<sup>th</sup> h) at a dose of 50 mg/kg body weight, 166.98±1.56 mg/dL (at 0 h) to 48.90±1.40 mg/dL (at 4<sup>th</sup> h) at a dose of 100 mg/kg body weight, and 168.64±2.96 mg/dL (at 0 h) to 42.46±1.80 mg/dL (at 4<sup>th</sup> h) at a dose of 150 mg/kg body weight, respectively. Similarly, the aqueous leaf extract of <em>O. europaea </em>subspecies <em>africana </em>significantly (p<0.05) reduced hyperglycemia from 165.74±3.66 mg/dL (at 0 h) to 65.26±1.46 mg/dL (at the 4<sup>th</sup> h) at a dose of 50 mg/kg body weight, 158.14±3.49 mg/dL (at 0 h) to 53.68±1.48 mg/dL (at the 4<sup>th</sup> h) at a dose of 100 mg/kg body weight, and from 161.66±2.19 mg/dL (at 0 h) to 44.48±1.35 mg/dL (at the 4<sup>th</sup> h) at a dose of 150 mg/kg body weight, respectively. Furthermore, the extracts contained chromium (Cr), zinc (Zn), magnesium (Mg), among other elements, and phytochemicals like phenols, flavonoids, and alkaloids, among others. Generally, the studied plant extracts exhibited significant hypoglycemic efficacy in alloxan-induced hyperglycemic mice, indicating their antidiabetic potential, and possess pharmacologically active phytochemicals and valuable minerals.</p>",
"DOI": "10.5455/jabet.2021.d134",
"views": 300,
"downloads": 67,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/07/06/178-1621847987-Figure1.jpg",
"caption": "Figure 1. Comparison between the blood glucose levels in experimental mice treated with the aqueous leaf extracts of P. zanzibarica and O. europaea subspecies africana measured at different periods. Bars with the same letter within the same dose level at each hour are not significantly different (p>0.05; Unpaired student t-test).",
"featured": false
}
],
"authors": [
{
"id": 1087,
"affiliation": [
{
"affiliation": "Mount Kenya University, Department of Medical Biochemistry, PO Box 342-01000, Thika-Kenya"
}
],
"first_name": "Gervason Apiri",
"family_name": "Moriasi",
"email": "gmoriasi@outlook.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Gervason Apiri Moriasi, Mount Kenya University, Department of Medical\r\nBiochemistry, PO Box 342-01000, Thika-Kenya, e-mail: gmoriasi@outlook.com",
"article": 235
},
{
"id": 1088,
"affiliation": [
{
"affiliation": "Technical University of Mombasa, Department of Pure and Applied Sciences, PO Box 90420-80100, Mombasa-Kenya"
}
],
"first_name": "Cromwell Mwiti",
"family_name": "Kibiti",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 235
},
{
"id": 1089,
"affiliation": [
{
"affiliation": "Kenyatta University, Department of Biochemistry, Microbiology, and Biotechnology, PO Box 43844-00100, Nairobi-Kenya"
}
],
"first_name": "Mathew Piero",
"family_name": "Ngugi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 235
}
]
},
{
"id": 230,
"slug": "178-1623169610-an-updated-review-on-lumpy-skin-disease-a-perspective-of-southeast-asian-countries",
"type": "review_article",
"manuscript_id": "178-1623169610",
"published": "2021-06-30",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/08/178-1623169610.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 322-333.",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Das M, Chowdhury MSR, et al. An updated review on lumpy skin disease: a perspective of Southeast Asian countries. J Adv Biotechnol Exp Ther. 2021; 4(3): 322-333.",
"title": "An updated review on lumpy skin disease: a perspective of Southeast Asian countries",
"abstract": "<p>Recently, Lumpy Skin Disease (LSD) has been portrayed as a terrifying threat to cattle in Southeast Asia. A lump like nodules in the external skin and mucous membrane with fever and swollen lymph nodes are the preliminary noticeable clinical signs of this devastating disease. It is commonly an arthropod-borne contagious illness, correspondingly the non-vector spreading through body discharge and infected fomites. The incubation period ranges from one to four weeks leading to viremia. A pronounced socio-economic collapse is driven by reduced quantity and quality of milk, udder infection, thinness, low quality hides, loss of draught power, abortion, infertility, limitation to meat ingestion, higher morbidity, etc. Animals of any age and gender are susceptible to the disease. The morbidity rate varies according to the immune status of animals and frequency of mechanical vectors. Primarily the disease was endemic in most Sub-Saharan regions of Africa, consequently extent to Middle East, Europe, and Asia. In the South-Eastern part of Asia, the disease has first been introduced in Bangladesh in July 2019 followed by China, India, Nepal, Bhutan, Vietnam, Hong Kong and Myanmar. Bangladesh recorded the maximum attack rate in Chattogram whereas at Cuttack in India. Particular vulnerable locations of other countries are yet to be confirmed. There is no epidemiological proceeding considering the present LSD situation report from rest of Asia. Strict quarantine, vector control, and prophylactic vaccine might be the best remedy for limiting the risk factors of the disease. Future studies should be directed towards determining the true burden of LSD on livestock and its potential risk factors with the perspective of geographic distributions.</p>",
"DOI": "10.5455/jabet.2021.d133",
"views": 2206,
"downloads": 328,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/06/178-1623169610-Figure1.jpg",
"caption": "Figure 1. Map showing LSD outbreaks in South-East Asia. A) Bangladesh and neighboring countries; B) Map showing LSD outbreaks in Bangladesh: 1-Dhaka, 2-Mymensingh, 3-Chattogram, 4-Barishal, 5-Khulna, 6-Rangpur, 7-Rajshahi, 8-Sylhet, C) Map showing LSD outbreaks in India: 1-Cuttak, 2-Bhadrak, 3-Mayurbhanj, 4-Balasore, 5-Kendrapara, and D) Map showing LSD outbreaks in China: 1-Xinjiang.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/06/178-1623169610-Figure2.jpg",
"caption": "Figure 2. Epitome of possible modes of transmission of LSDV. LSD infected cattle may affect non-infected cattle through vector or non-vector transmission.",
"featured": false
}
],
"authors": [
{
"id": 1057,
"affiliation": [
{
"affiliation": "Department of Epidemiology and Public Health, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Moumita",
"family_name": "Das",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 230
},
{
"id": 1058,
"affiliation": [
{
"affiliation": "Department of Medicine, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Shahidur Rahman",
"family_name": "Chowdhury",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 230
},
{
"id": 1059,
"affiliation": [
{
"affiliation": "Department of Epidemiology and Public Health, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Sharmin",
"family_name": "Akter",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 230
},
{
"id": 1060,
"affiliation": [
{
"affiliation": "Department of Physiology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Apurbo Kumar",
"family_name": "Mondal",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 230
},
{
"id": 1061,
"affiliation": [
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea"
},
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 230
},
{
"id": 1062,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, East Azampur, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Department of Pathology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md. Masudur",
"family_name": "Rahman",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 230
},
{
"id": 1063,
"affiliation": [
{
"affiliation": "Department of Medicine, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Md Mahfujur",
"family_name": "Rahman",
"email": "mahfuj.vetmed@sau.ac.bd",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Mahfujur Rahman, PhD; Department of Medicine, Sylhet Agricultural University, Sylhet3100, Bangladesh, e-mail: mahfuj.vetmed@sau.ac.bd",
"article": 230
}
]
},
{
"id": 229,
"slug": "178-1613826663-prevalence-and-population-biology-of-mastitis-causing-streptococcus-uberis-using-an-mlst-based-approach",
"type": "original_article",
"manuscript_id": "178-1613826663",
"published": "2021-06-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/46/178-1613826663.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 311-321.",
"academic_editor": "Md. Abdul Hannan, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Rahman A, Bhattacharjee A, et al. Prevalence and population biology of mastitis-causing Streptococcus uberis using an MLST based approach. J Adv Biotechnol Exp Ther. 2021; 4(3): 311-321.",
"title": "Prevalence and population biology of mastitis-causing Streptococcus uberis using an MLST based approach",
"abstract": "<p><em>Streptococcus uberis</em> is a predominant causative agent of both clinical and subclinical varieties of bovine mastitis, an economically significant infection affecting dairy industries around the world. Yet, the genetic and evolutionary relationships among <em>S. uberis</em> strains from different countries are poorly understood. In this study, we used <em>S. uberis</em>’s multilocus sequence typing (MLST) method of genotyping to decipher country-wise prevalence of sequence types (STs) and ST complexes and to delineate genetic relationships among them. Dataset collected from PubMLST database for <em>S. uberis</em> was subjected to clonal cluster and phylogenetic analyses using BURST, globally optimized eBURST (goeBURST) and neighborhood joining algorithm tools, respectively. Whereas certain countries showed clear trends of strain prevalence, others had a more equally weighted, diverse population. Occurrence of different severities of disease varied among countries and displayed no direct correlation with ST. Clonal cluster and phylogenetic analyses predicted the ancestral roles of certain prominent STs and indicated possible strain migration and reticulate and convergent evolution occurring within the <em>S. uberis </em>population at a global scale. Furthermore, genome comparison of selected strains revealed the absence of SUB0822-SUB0826 response regulator proteins from ST-5 and ST-6 indicating their preference for contagious transmission. Information generated from this study would be crucial for monitoring infection outbreaks and directing further genomic investigations.</p>",
"DOI": "10.5455/jabet.2021.d132",
"views": 865,
"downloads": 144,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/07/178-1613826663-Figure1.jpg",
"caption": "Figure 1. a) Frequency of most prevalent S. uberis STs by country. Only countries with 30 or more isolates have been represented. b) Frequency of most prevalent S. uberis STs by disease state. (Note: For each country or disease state, up to 5 most prevalent STs have been depicted. The numbers in brackets “{}” at the top represent the total number of isolates for each group).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/07/178-1613826663-Figure2.jpg",
"caption": "Figure 2. Clonal clusters of strains from the UK, New Zealand and Sweden. Key: grey circle= ancestral ST; red circle= single locus variant (SLV); blue circle= double locus variant (DLV), outside circle= triple locus variant (TLV). TLVs shared between distinct clonal clusters are marked by red circles. The figure was generated using PubMLST’s BURST tool.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/07/178-1613826663-Figure3.jpg",
"caption": "Figure 3. goeBURST analysis showing predicted evolutionary linkage between strains from different geographical regions. It predicts an evolutionary pathway leading from ST-5, -6 and -233 predominantly found in the UK to ST-184 and ST-91 that frequently occur in New Zealand. Two distinct zones of cluster linkages have been indicated. A few STs clustering with a foreign ancestral ST has been labeled by country name.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/07/178-1613826663-Figure4.jpg",
"caption": "Figure 4. A neighbor-joining (NJ) tree of all S. uberis strains visualized through iTOL. Locations of prevalent strains from countries with high number of isolates have been indicated (ST-5, -6, -20, -24 and -26 are prevalent in the UK while ST-85, ST-91, ST-114 and -184 are prevalent in New Zealand).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/07/178-1613826663-Figure5.jpg",
"caption": "Figure 5. Frequency of polymorphic sites and alleles for the 7 housekeeping genes involved in the MLST scheme of S. uberis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/07/178-1613826663-Figure6.jpg",
"caption": "Figure 6. Protein-protein interaction of SUB822, SUB0823, SUB0824 (malP), SUB0825 with each other and virulent protein SUB0826. The functions of proteins interacting with SUB0825 (Idh, ppdK, pdhB, pyk and pta) have been indicated.",
"featured": false
}
],
"authors": [
{
"id": 1052,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Baridhara, Basundhara, Dhaka 1229, Bangladesh"
},
{
"affiliation": "NSU Genome Research Institute (NGRI), North South University, Baridhara, Basundhara, Dhaka 1229, Bangladesh"
}
],
"first_name": "Aura",
"family_name": "Rahman",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 229
},
{
"id": 1053,
"affiliation": [
{
"affiliation": "NSU Genome Research Institute (NGRI), North South University, Baridhara, Basundhara, Dhaka 1229, Bangladesh"
}
],
"first_name": "Arittra",
"family_name": "Bhattacharjee",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 229
},
{
"id": 1054,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Baridhara, Basundhara, Dhaka 1229, Bangladesh"
},
{
"affiliation": "NSU Genome Research Institute (NGRI), North South University, Baridhara, Basundhara, Dhaka 1229, Bangladesh"
}
],
"first_name": "Tahmina",
"family_name": "Tabassum",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 229
},
{
"id": 1055,
"affiliation": [
{
"affiliation": "Department of Microbiology, Jagannath University, Dhaka 1100, Bangladesh"
}
],
"first_name": "Mohammad Ariful",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 229
},
{
"id": 1056,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Microbiology, North South University, Baridhara, Basundhara, Dhaka 1229, Bangladesh"
},
{
"affiliation": "NSU Genome Research Institute (NGRI), North South University, Baridhara, Basundhara, Dhaka 1229, Bangladesh"
}
],
"first_name": "Maqsud",
"family_name": "Hossain",
"email": "muhammad.maqsud@northsouth.edu",
"author_order": 5,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Maqsud Hossain, PhD; NSU Genome Research Institute (NGRI), North South University, Baridhara, Bashundhara, Dhaka-1229, Bangladesh, e-mail:\r\nmuhammad.maqsud@northsouth.edu",
"article": 229
}
]
},
{
"id": 228,
"slug": "178-1620556156-aberrant-methylation-of-cdkn2a-rassf1a-and-wif1-in-sporadic-adenocarcinomatous-colorectal-cancer-associations-with-clinicopathological-features",
"type": "short_communication",
"manuscript_id": "178-1620556156",
"published": "2021-06-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/28/178-1620556156.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 305-310.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea.",
"cite_info": "Vuong LD, Nguyen HV, et al.Aberrant methylation of CDKN2A, RASSF1A and WIF1 in sporadic adenocarcinomatous colorectal cancer: Associations with clinicopathological features. J Adv Biotechnol Exp Ther. 2021; 4(3): 305-310.",
"title": "Aberrant methylation of CDKN2A, RASSF1A and WIF1 in sporadic adenocarcinomatous colorectal cancer: Associations with clinicopathological features",
"abstract": "<p>Accumulating evidence support that aberrant methylation of various cancer-related genes plays an important role in the initiation and progression of colorectal cancer (CRC). This study aims to validate the accuracy of methylation specific polymerase chain reaction (MSP) to assess frequency and distribution of <em>GSTP1</em>, <em>CDKN2A</em>, <em>RASSF1A</em>, and <em>WIF1</em> methylation and analyse their correlation with clinicopathological variables in sporadic adenocarcinomatous CRC. Of the 248 CRC tissues, methylation was identified in 7.7% for <em>GSTP1</em>, 22.2% for <em>CDKN2A</em>, 33.1% for <em>RASSF1A</em>, and 54.4% for <em>WIF1</em>. Hypermethylation of <em>CDKN2A</em>, <em>RASSF1A</em>, and <em>WIF1</em> was significantly associated with adenocarcinoma (<em>p</em>< 0.001), mucinous adenocarcinoma (<em>p</em>< 0.001), and signet-ring cell adenocarcinoma subtypes (<em>p</em> = 0.017), respectively. Both <em>CDKN2A</em> and <em>WIF1</em> methylations were more common in stage II (<em>p</em> = 0.012 for <em>CDKN2A</em> and <em>p</em> = 0.010 for <em>WIF1</em>) and absence of lymph node metastasis (<em>p</em> = 0.011 for <em>CDKN2A </em>and <em>p</em> = 0.012 for <em>WIF1</em>) but were less common in stage III (<em>p</em> = 0.016 for <em>CDKN2A</em> and <em>p</em> = 0.010 for <em>WIF1</em>). <em>RASSF1A</em> methylation was associated with moderate differentiation (<em>p</em> = 0.038). These findings suggest that methylation of <em>CDKN2A</em>, <em>RASSF1A</em>, and <em>WIF1 </em>may significantly contribute to CRC pathogenesis and may be considered as valuable biomarkers for accessing the development and progression of particular subtypes of colorectal cancer.</p>",
"DOI": "10.5455/jabet.2021.d131",
"views": 551,
"downloads": 126,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/07/178-1620556156-Figure1.jpg",
"caption": "Figure 1. Representative analysis of MSP products amplified from bisulfite treated DNA with the primer sets of GSTP1 (A), RASSF1A (B), WIF1 (C) and CDKN2A (D). L: 100bp DNA ladder. (-): Negative control without DNA templates. S: colorectal cancer samples.",
"featured": false
}
],
"authors": [
{
"id": 1048,
"affiliation": [
{
"affiliation": "Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam"
}
],
"first_name": "Linh Dieu",
"family_name": "Vuong",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 228
},
{
"id": 1049,
"affiliation": [
{
"affiliation": "Medical Image Technology Laboratory, Department of Computer Engineering, College of Engineering, Inje University, Gimhae 50834,\r\nSouth Korea"
}
],
"first_name": "Hung Viet",
"family_name": "Nguyen",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 228
},
{
"id": 1050,
"affiliation": [
{
"affiliation": "Department of Smart Food and Drug, College of BNIT, Inje University, Gimhae 50834, South Korea"
}
],
"first_name": "Van-Long",
"family_name": "Truong",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 228
},
{
"id": 1051,
"affiliation": [
{
"affiliation": "Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam"
}
],
"first_name": "Quang Ngoc",
"family_name": "Nguyen",
"email": "quangk8s@gmail.com",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Quang Ngoc Nguyen, PhD; Pathology and Molecular Biology Center, National Cancer Hospital K, 30 Cau Buou Street, Thanh Tri, Hanoi, Vietnam, e-mail: quangk8s@gmail.com",
"article": 228
}
]
},
{
"id": 215,
"slug": "178-1618847123-comparison-of-different-chronic-respiratory-diseases-in-terms-of-cigarette-smoking-a-hospital-based-case-control-study-on-population-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1618847123",
"published": "2021-06-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/07/178-1618847123.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 298-304.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea.",
"cite_info": "Das A, Sumit AF, et al. Comparison of different chronic respiratory diseases in terms of cigarette smoking: A hospital-based case control study on population of Bangladesh. J Adv Biotechnol Exp Ther. 2021; 4(3): 298-304.",
"title": "Comparison of different chronic respiratory diseases in terms of cigarette smoking: A hospital-based case control study on population of Bangladesh",
"abstract": "<p>Cigarette smoking is considered as one of the main factors of developing chronic respiratory diseases (CRDs), but very few studies had been performed showing comparative association between different CRDs and cigarette smoking. This study attempts to determine and compare the association of cigarette smoking with different CRDs including chronic obstructive pulmonary disease (COPD), chronic bronchitis (CB), and asthma altogether in the context of Bangladesh. This case-control study was conducted among 406 subjects, where 297 were cases of chronic respiratory illness and 109 were controls. The diagnosis of each respiratory diseases (either asthma or CB or COPD) was confirmed by registered physicians. Of 98 COPD patients, 100 CB patients, 99 asthma patients and 109 controls, current smokers were found as 67.3%, 58%, 41.4%, and 33.9%, respectively. Current smokers with Brinkman Index (BI)≥ 400 were 43.9%, 39.7%, 26.9% and 8.2% for COPD, CB, asthma, and control group, sequentially. Regarding unadjusted association between cigarette smoking and different CRDs, current smokers with BI≥ 400 had 4.55, 21.75 and 13.14 times significantly higher chances of developing asthma, COPD and CB respectively than non-smokers. Logistic analysis revealed that after adjustment with age, body mass index (BMI) and gender, current smokers with BI≥ 400 had 6.01, 15.63 and 33.47 times more likelihood of developing asthma, CB, and COPD respectively than non-smokers; and these findings were significant as well. Thus, it can be concluded that cigarette smoking affected COPD more pronouncedly followed by CB and asthma among the study population.</p>",
"DOI": "10.5455/jabet.2021.d130",
"views": 1095,
"downloads": 171,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/07/178-1618847123-Figure1.jpg",
"caption": "Figure 1. Association of cigarette smoking with asthma, COPD and CB. The odds ratio (ORs) with 95% confidence interval (CI) of having asthma, COPD and CB among the non-smokers (BI=0) and current smokers (BI<400 and BI≥ 400) were shown. Current smokers with BI≥ 400 showed significantly higher ORs for asthma, COPD and CB compared to non-smokers, while current smokers with BI< 400 showed significantly higher ORs for COPD only compared to non-smokers (BI=0).",
"featured": false
}
],
"authors": [
{
"id": 979,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000"
}
],
"first_name": "Anindya",
"family_name": "Das",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 215
},
{
"id": 980,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000"
}
],
"first_name": "Ahmed Faisal",
"family_name": "Sumit",
"email": "ahmedfaisalsumit@du.ac.bd",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ahmed Faisal Sumit, Department of Genetic Engineering and Biotechnology, University of Dhaka, Bangladesh. Email: ahmedfaisalsumit@du.ac.bd",
"article": 215
},
{
"id": 981,
"affiliation": [
{
"affiliation": "Department of Respiratory Medicine, Dhaka Medical College Hospital, Dhaka, Bangladesh"
}
],
"first_name": "Debasish",
"family_name": "Bhowmick",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 215
}
]
},
{
"id": 212,
"slug": "178-1618595762-management-of-mental-health-during-covid-19-pandemic-possible-strategies",
"type": "review_article",
"manuscript_id": "178-1618595762",
"published": "2021-06-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/57/178-1618595762.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 276-289.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea.",
"cite_info": "Sohel M, Hossain MA, et al. Management of mental health during COVID-19 pandemic: possible strategies. J Adv Biotechnol Exp Ther. 2021; 4(3): 276-289.",
"title": "Management of mental health during COVID-19 pandemic: possible strategies",
"abstract": "<p>In the 21<sup>st</sup> century, any pandemic, especially, SARS-CoV-2 is a global burden due to high incidence, mortality, and mutation rate. Although several techniques have already been identified to control the pandemic or treat patients and causes of adverse impact on mental health, relatively only, fewer researchers have little concern about finding effective mitigation strategies to improve mental health. Therefore, this study aimed to find some common and unique approaches to manage mental health during a pandemic. Some strategies for the better management of mental health induced by SARS-CoV-2 infections are required for all classes of peoples. Early management is vital, and those must be associated with frontline workers and people staying at home, particularly in isolation centers and already identified as active cases. Experts have pointed out the need to pay specific attention to proper daily life. To manage abnormal mental conditions, such as anxiety, mood, personality, and psychotic disorder during the pandemic; social media, meditation, and psychological motivation with adequate diet, exercise, and sleep have significant roles in regulating some biological mechanism, incredibly immune, hormonal, and neural process. Management of mental health is mandatory for all at the time of the SARS-CoV-2 pandemic. We can consider all of the strategies mentioned above to treat mental health during and after the COVID-19 pandemic condition.</p>",
"DOI": "10.5455/jabet.2021.d128",
"views": 2183,
"downloads": 147,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/20/07/178-1618595762-Figure1.jpg",
"caption": "",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/20/07/178-1618595762-Figure2.jpg",
"caption": "",
"featured": false
}
],
"authors": [
{
"id": 952,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Md",
"family_name": "Sohel",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 212
},
{
"id": 953,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Md. Arju",
"family_name": "Hossain",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 212
},
{
"id": 954,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Mohammad Kamrul",
"family_name": "Hasan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 212
},
{
"id": 955,
"affiliation": [
{
"affiliation": "Bangabandhu Sheikh Mujib Medical College, University of Dhaka, Faridpur 7800, Bangladesh"
}
],
"first_name": "Anonna",
"family_name": "Haque",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 212
},
{
"id": 956,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Islamic University, Kushtia7003, Bangladesh"
}
],
"first_name": "Md. Shoriful",
"family_name": "Islam",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 212
},
{
"id": 957,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka-1230. Bangladesh"
}
],
"first_name": "Md. Shahadat",
"family_name": "Hossain",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 212
},
{
"id": 958,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka-1230. Bangladesh"
},
{
"affiliation": "Pratyasha Health Biomedical Research Center, Dhaka-1230, Bangladesh"
}
],
"first_name": "Mohammad Nurul",
"family_name": "Amin",
"email": "amin.pharma07@gmail.com",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Nurul Amin, Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka-1230. Bangladesh. Email: amin.pharma07@gmail.com",
"article": 212
},
{
"id": 959,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh"
}
],
"first_name": "Talha Bin",
"family_name": "Emran",
"email": "talhabmb@bgctub.ac.bd",
"author_order": 8,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran, PhD; Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh. Email: talhabmb@bgctub.ac.bd",
"article": 212
}
]
},
{
"id": 211,
"slug": "178-1615318554-evaluation-of-optimum-dietary-inclusion-level-of-probiotics-for-potential-benefits-on-intestinal-histomorphometry-microbiota-and-ph-in-japanese-quails",
"type": "original_article",
"manuscript_id": "178-1615318554",
"published": "2021-06-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/15/178-1615318554.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 265-275.",
"academic_editor": "Md Nabiul Islam, PhD; Yamaguchi University, Japan",
"cite_info": "Afrin M, Sachi MS, et al. Evaluation of optimum dietary inclusion level of probiotics for potential benefits on intestinal histomorphometry, microbiota, and pH in Japanese Quails. J Adv Biotechnol Exp Ther. 2021; 4(3): 265-275.",
"title": "Evaluation of optimum dietary inclusion level of probiotics for potential benefits on intestinal histomorphometry, microbiota, and pH in Japanese Quails",
"abstract": "<p>Among the alternative options of antibiotics as growth promoters (AGP) to reduce the antimicrobial resistance, probiotics are the attractive alternative which needs to compare at different doses with AGP on the intestinal health of Japanese quail. For this, a total 75 Japanese quails were equally assigned to five treatment groups having three replicates in each group (n=5). In addition to basal diet (control), four other groups were supplemented by AGP and probiotics at the dose of 0.015 gm/bird, 0.03 gm/bird, and 0.045 gm/bird. The results revealed, 0.03 gm/bird probiotics group had significantly (p<0.05) lower mean on gizzard and intestine relative weights (gm/kg) of 23.68 and 35.61; and the relative length (cm/kg) of duodenum, jejunum and ileum were 51.06, 137.30 and 101.95, respectively. Additionally, the villus height (VH) of jejunum and ileum had significantly (p<0.01) higher mean in 0.03 gm/bird probiotics group of 599.25 and 417.25 µm, respectively. Although, there was a quadratic relationship in VH of jejunum (p<0.001) and ileum (p<0.01), CD (p<0.01) and VH:CD (p<0.05) of duodenum with the probiotics dose, but only VH of jejunum and ileum (p<0.001) showed a linear interaction. The enumeration of intestinal bacteria was lower in AGP group but did not differ significantly (p>0.05) with 0.03 gm/bird probiotics group in which the <em>E. coli</em>, <em>Salmonella, Staphylococcus</em> and TBC mean was 5.160 log<sub>10</sub>, 4.440 log<sub>10</sub>, 2.923 log<sub>10</sub> and 6.972 log<sub>10</sub> CFU/gm, respectively. However, the highest pH was recorded in ileum in each group without any significant differences. In a short of, probiotics are effective substitute to AGP and having the potential effects on intestinal health especially for 0.03 gm/bird.</p>",
"DOI": "10.5455/jabet.2021.d127",
"views": 995,
"downloads": 103,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/07/178-1615318554-Figure1.png",
"caption": "Figure 1. Histological representation of duodenal mucosa of control and 0.03 mg/bird probiotic treated groups in Japanese quails. The yellow lines and red lines represent villus height, and crypt depth respectively (H&E, ×10). VH: villus height; CD: crypt depth. Scale bar = 200 µm. The blue arrow showing duodenal villi (H&E, ×40; Scale bar = 50 µm) in control and 0.03 mg/bird probiotic treated groups of Japanese quails.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/07/178-1615318554-Figure2.jpg",
"caption": "Figure 2. Histological representation of jejunum mucosa of control and 0.03 mg/bird probiotic treated groups in Japanese quails. The yellow lines and red lines represent villus height and crypt depth respectively (H&E, ×40). VH: villus height; CD: crypt depth. Scale bars, 50 µm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/07/178-1615318554-Figure3.jpg",
"caption": "Figure 3. Histological representation of ileum mucosa of control and 0.03 mg/bird probiotic treated groups in Japanese quails. The yellow lines and red lines represent villus height and crypt depth respectively (H&E, ×40). VH: villus height; CD: crypt depth. Scale bars, 50 µm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/07/178-1615318554-Figure4.jpg",
"caption": "Figure 4. The pH values of Duodenum, Jejunum and Ileum of control, probiotics and AGP groups in Japanese quails. Data are presented in boxplot. Each boxplot represents the average value for all the data points (control and challenged groups, n = 30). Significant differences were determined using One-Way Analysis of Variance (ANOVA) with Duncan’s multiple range tests. A higher trend of pH was observed in ileum and lower trend in duodenum of all treated and control groups. pH level of duodenum, jejunum and ileum of control and challenged groups had no significant (p>0.05) differences.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/07/178-1615318554-Figure5.jpg",
"caption": "Figure 5. A schematic summary showing probiotics as effective substitute to AGP in a dose dependent manner. In experiment, Japanese quails (n=75) were randomly allocated to five treatment groups having three replicates in each group (n=5). In addition to basal diet (control), four other groups were supplemented by AGP (0.5 gm/50kg) and probiotics at the dose of 0.015 gm/bird, 0.03 gm/bird, and 0.045 gm/bird. After 49 days of study, birds were sacrificed for pH measurement, enumeration of intestinal microbiota and intestinal histomorphometric analysis. The present study demonstrates the alteration of intestinal pH which are not accompanied by the probiotic supplementation, whereas dietary inclusion of probiotics in quails can increase the VH of Jejunum, ileum, and also CD of duodenum significantly compared to control and AGP. Moreover, probiotics at 0.03 gm/bird decreased pathogenic intestinal bacteria count in relation to AGP. Hence, our findings denote that the probiotics inclusion, especially @ 0.03 gm/bird, outweigh AGP to enhance intestinal health in Japanese quails.",
"featured": false
}
],
"authors": [
{
"id": 948,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Marya",
"family_name": "Afrin",
"email": "marya.afrin@bau.edu.bd",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ms. Marya Afrin, Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Email: marya.afrin@bau.edu.bd",
"article": 211
},
{
"id": 949,
"affiliation": [
{
"affiliation": "Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Sabbya",
"family_name": "Sachi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 211
},
{
"id": 950,
"affiliation": [
{
"affiliation": "Department of Microbiology and Public Health, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Mirza Mienur",
"family_name": "Meher",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 211
},
{
"id": 951,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Nusrat",
"family_name": "Jahan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 211
}
]
},
{
"id": 214,
"slug": "178-1620111160-antiviral-effect-of-honey-extract-camelyn-against-sars-cov-2",
"type": "original_article",
"manuscript_id": "178-1620111160",
"published": "2021-06-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/28/178-1620111160.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 290-297.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Kalediene L, Baz M, et al. Antiviral effect of honey extract Camelyn against SARS-CoV-2. J Adv Biotechnol Exp Ther. 2021; 4(3): 290-297.",
"title": "Antiviral effect of honey extract Camelyn against SARS-CoV-2",
"abstract": "<p>This study aimed to evaluate the potential antiviral effects of honey extract “Camelyn” against Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2). The baby hamster kidney cell line 21 (BHK‐21), bone marrow-derived hematopoietic stem cells (HSCs), and splenic cells were used for Camelyn cytotoxicity assay. After the isolation procedures, cell viability was assessed by trypan blue dye exclusion under a microscope using a hemocytometer. The in vitro cell growth rate was carried out using the cell counting Kit 8 (CCK-8) assay. The cells were seeded in growth media with various Camelyn concentrations (35 μg, 50 μg, 70 μg, 100 μg, 150 μg, and 200 μg). The absorbance at 450 nm was determined by the multiplate reader. The antiviral effect was assessed by plaque reduction assay for the determination of drug susceptibility against SARS-CoV-2. Serial dilution of the selected compounds was pre-incubated with 40 to 100 plaque-forming units (PFUs) of SARS-CoV-2. The pre-incubated mix of Camelyn and SARS-CoV-2 was then added to the confluent Vero E6 cells after incubation cells were fixed and stained and the number of PFUs was counted under an inverted microscope and plotted against the logarithm of antiviral concentrations. Our study showed that Camelyn is not cytotoxic, has a stimulatory effect on cell proliferation, and has an inhibitory effect against SARS-CoV-2 with EC50 (half-maximal effective concentration) from 85.7 μg/mL to 192.4 μg/mL depending on product concentration and viral plaque per cell.</p>",
"DOI": "10.5455/jabet.2021.d129",
"views": 4846,
"downloads": 167,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/07/178-1620111160-Figure1.jpg",
"caption": "Figure 1. Influence of different concentrations of honey extract Camelyn on baby hamster kidney cell line 21 (BHK‐21) (a), hematopoietic stem cells (HSCs) (b), and spleen cells (c) growth; black horizontal line – a value of control OD.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/07/178-1620111160-Figure2.jpg",
"caption": "Figure 2. Effect of different Camelyn concentrations on SARS-CoV-2 plaque reduction in VERO E6 cells: blue line – average virus plaque per well was 100 (Spearman rank correlations between virus plaque per well and the concentration is rs1 = -1.000, and significant at the 0.01 level (2-tailed)).; red line – average virus plaque per well was 30 (Spearman rank correlations between virus plaque per well and the concentration rs2 = -0.952, are significant at the 0.01 level (2-tailed)); grey line – half-maximal effective concentration (EC50).",
"featured": false
}
],
"authors": [
{
"id": 969,
"affiliation": [
{
"affiliation": "Department of Microbiology and Biotechnology of Life Sciences Centre of Vilnius University, Lithuania"
}
],
"first_name": "Lilija",
"family_name": "Kalediene",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 214
},
{
"id": 970,
"affiliation": [
{
"affiliation": "Research Center in Infectious Diseases, CHU of Québec-Laval University, Québec City, QC, Canada"
}
],
"first_name": "Mariana",
"family_name": "Baz",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 214
},
{
"id": 971,
"affiliation": [
{
"affiliation": "Pharmacy Center, Institute of Biomedical Sciences, Faculty of Medicine, University of Vilnius, Lithuania"
},
{
"affiliation": "Department of Biomodels of State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania"
}
],
"first_name": "Ausra",
"family_name": "Liubaviciute",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 214
},
{
"id": 972,
"affiliation": [
{
"affiliation": "Department of Biomodels of State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania"
}
],
"first_name": "Gene",
"family_name": "Biziuleviciene",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 214
},
{
"id": 973,
"affiliation": [
{
"affiliation": "Department of Physics, Mathematics and Biophysics of Lithuanian University of Health Sciences, Kaunas, Lithuania"
}
],
"first_name": "Ingrida",
"family_name": "Grabauskyte",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 214
},
{
"id": 974,
"affiliation": [
{
"affiliation": "UAB \"Silicio Biotechnologijos\", Vilnius, Lithuania"
}
],
"first_name": "Ruta",
"family_name": "Bieliauskiene",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 214
},
{
"id": 975,
"affiliation": [
{
"affiliation": "UAB \"Silicio Biotechnologijos\", Vilnius, Lithuania"
}
],
"first_name": "Paulius",
"family_name": "Jovaisas",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 214
},
{
"id": 976,
"affiliation": [
{
"affiliation": "UAB “Guruma”, Kaunas, Lithuania"
}
],
"first_name": "Nidas",
"family_name": "Jurjonas",
"email": "nidas.jurjonas@guruma.eu",
"author_order": 8,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nidas Jurjonas, UAB “Guruma”, Kaunas, Lithuania, Phone: +370-37-328 877. Email: nidas.jurjonas@guruma.eu",
"article": 214
}
]
},
{
"id": 199,
"slug": "178-1618063128-prevalence-of-multidrug-resistance-patterns-of-escherichia-coli-from-suspected-urinary-tract-infection-in-mymensingh-city-bangladesh",
"type": "original_article",
"manuscript_id": "178-1618063128",
"published": "2021-06-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/17/178-1618063128.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(3): 256-264.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Nobel FA, Akter S, et al. Prevalence of multidrug resistance patterns of Escherichia coli from suspected urinary tract infection in Mymensingh city, Bangladesh. J Adv Biotechnol Exp Ther. 2021; 4(3): 256-264.",
"title": "Prevalence of multidrug resistance patterns of Escherichia coli from suspected urinary tract infection in Mymensingh city, Bangladesh",
"abstract": "<p>Uropathogenic <em>Escherichia coli </em>(UPEC) is considered as one of the major bacteria causing urinary tract infection (UTI) affecting millions of people worldwide. UPEC rates of high resistance towards antibiotics have increased dramatically in recent years and made treatment difficult in Bangladesh. The study intended to determine the prevalence and antibiotic resistance pattern of <em>E. coli</em> from suspected urinary tract infections in Bangladeshi patients. A single cross-sectional retrospective observation was carried out in the Department of Microbiology at the Popular Diagnostic Centre, Mymensingh city, Bangladesh from August 2019 to August 2020. We collected data on urine culture from diagnostic reports of 4000 patients from which positive urine culture data were analyzed using SPSS software. During the study period, 453 positive urine cultures were identified from 4000 suspected UTI patients. Among them, 300 (66.2%) were female and 153 (33.8%) were male with their mean age of 45.50 (47.94 for male and 44.27 for female). According to the findings, <em>Escherichia coli</em> was the only uropathogenic bacterial species found in the patient’s urine culture. The highest antimicrobial resistance was seen among patients aged between 41 and 50 years. In an antimicrobial susceptibility test, 99% of isolates were resistant to at least one antibiotic, and 92% were multidrug-resistant (≥3 classes).</p>",
"DOI": "10.5455/jabet.2021.d126",
"views": 1445,
"downloads": 204,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/07/178-1618063128-Figure1.jpg",
"caption": "Figure 1. Pie diagram shows the gender distribution of male and female patients. From 453 patients, 153 (33.8%) were male and 300 (66.2%) were female patients.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/07/178-1618063128-Figure2.jpg",
"caption": "Figure 2. The number of male & female patients according to their age groups.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/07/178-1618063128-Figure3.jpg",
"caption": "Figure 3. Orange, aqua, and purple color bars show the percentages of resistant, intermediate and sensitivity patterns of antibiotics against E. coli, respectively.",
"featured": false
}
],
"authors": [
{
"id": 936,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Fahim Alam",
"family_name": "Nobel",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 937,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Sharmin",
"family_name": "Akter",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 938,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Ruksana Akter",
"family_name": "Jebin",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 939,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Titash Chandra",
"family_name": "Sarker",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 940,
"affiliation": [
{
"affiliation": "Department of Statistics, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Md. Mizanur",
"family_name": "Rahman",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 941,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Saad Al",
"family_name": "Zamane",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 942,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Khairul",
"family_name": "Islam",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 943,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Saima",
"family_name": "Sabrina",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 944,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Nishat",
"family_name": "Akther",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 945,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Ashekul",
"family_name": "Islam",
"email": null,
"author_order": 10,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 946,
"affiliation": [
{
"affiliation": "Popular Diagnostic Centre, Mymensingh, Bangladesh"
}
],
"first_name": "Md. Rakibul",
"family_name": "Hasan",
"email": null,
"author_order": 11,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 199
},
{
"id": 947,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Mohammod Johirul",
"family_name": "Islam",
"email": "johir7479@gmail.com",
"author_order": 12,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammod Johirul Islam, PhD; Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh. Email: johir7479@gmail.com",
"article": 199
}
]
}
]
},
{
"id": 14,
"title": "Vol4 Issue2",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2021",
"slug": "2021-vol4-issue2",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 185,
"slug": "178-1617798303-detection-of-multidrug-resistant-salmonella-spp-from-healthy-and-diseased-broilers-having-potential-public-health-significance",
"type": "original_article",
"manuscript_id": "178-1617798303",
"published": "2021-05-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/48/178-1617798303.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 248-255.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "",
"title": "Detection of multidrug resistant Salmonella spp. from healthy and diseased broilers having potential public health significance",
"abstract": "<p>Multidrug resistant (MDR) <em>Salmonella</em> spp. poses significant global public health concern by causing food-borne infections. This study aimed to detect MDR <em>Salmonella </em>spp<em>.</em> from healthy and diseased broiler chickens in the Mymensingh and Jamalpur districts of Bangladesh. Total 70 samples comprising feces (n=20), chicken meat (n=30), and visceral organs i.e. liver, lung, and kidney (n=20) were collected. <em>Salmonella</em> were isolated and identified by culture, biochemical tests and PCR. The antibiogram study was performed by the disk diffusion method. By PCR, 30% (21/70; 95% CI: 19.32-40.05%) samples were positive for <em>Salmonella </em>spp., of which significantly (p=0.005) higher occurrence were detected in feces (50%; 95% CI: 29.93-70.07%) compared to chicken meat (10%; 95% CI: 3.46-25.62%) and visceral organs (40%; 95% CI: 21.88-61.34%). By antibiogram, all the <em>Salmonella</em> isolates were resistant to amoxicillin, and frequently (90.48-19.05%) resistant to tetracycline, ceftazidime, chloramphenicol, colistin, and ciprofloxacin. The significantly higher resistance of chloramphenicol, tetracycline, and ceftazidime were observed in the internal organs of broilers. Interestingly, 80.95% (17/21; 95% CI: 59.99-92.33%) <em>Salmonella</em> isolates were MDR in nature. The range of multiple antibiotic resistance (MAR) index of <em>Salmonella</em> isolates varied from 0.29 to 0.86. The high occurrence of MDR and MAR <em>Salmonella</em> in broilers detected in our present study could reveal a high risk to public health and these organisms could be transmitted to humans through the food supply. We suggest that effective prevention and control measures should be implemented to reduce their potential contamination and to minimize the emergence of antibiotic resistance.</p>",
"DOI": "10.5455/jabet.2021.d125",
"views": 1471,
"downloads": 105,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/15/04/178-1617798303-Figure1.jpg",
"caption": "Figure 1. Study area map produced by ArcMap (version 10.7) software (ESRI, Redlands, CA, USA).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/15/04/178-1617798303-Figure2.jpg",
"caption": "Figure 2. Antibiogram profiles of Salmonella isolated from broiler samples. Here, AMX= Amoxicillin, CIP= Ciprofloxacin, C= Chloramphenicol, CL= Colistin, GEN= Gentamicin, TE= Tetracycline, CAZ= Ceftazidime.",
"featured": false
}
],
"authors": [
{
"id": 788,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mithun",
"family_name": "Talukder",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 185
},
{
"id": 789,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Saiful",
"family_name": "Islam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 185
},
{
"id": 790,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Samina",
"family_name": "Ievy",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 185
},
{
"id": 791,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Abdus",
"family_name": "Sobur",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 185
},
{
"id": 792,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Fatimah Mohammed",
"family_name": "Ballah",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 185
},
{
"id": 793,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md.",
"family_name": "Najibullah",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 185
},
{
"id": 794,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Bahanur",
"family_name": "Rahman",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 185
},
{
"id": 795,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Tanvir",
"family_name": "Rahman",
"email": "tanvirahman@bau.edu.bd",
"author_order": 8,
"ORCID": "https://orcid.org/0000-0001-5432-480X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Tanvir Rahman, PhD, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Email: tanvirahman@bau.edu.bd",
"article": 185
},
{
"id": 796,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mohammad Ferdousur Rahman",
"family_name": "Khan",
"email": "frkhanbau80@yahoo.com",
"author_order": 9,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Ferdousur Rahman\r\nKhan, PhD; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. e-mail: frkhanbau80@yahoo.com",
"article": 185
}
]
},
{
"id": 184,
"slug": "178-1619144787-exercise-and-oral-melatonin-attenuate-anxiety-and-depression-like-behavior-in-type-2-diabetic-rats",
"type": "original_article",
"manuscript_id": "178-1619144787",
"published": "2021-05-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/15/178-1619144787.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 238-247.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea.",
"cite_info": "Rahman MM, Park SJ, et al. Exercise and oral melatonin attenuate anxiety and depression like behavior in type 2 diabetic rats. J Adv Biotechnol Exp Ther. 2021; 4(2): 238-247.",
"title": "Exercise and oral melatonin attenuate anxiety and depression like behavior in type 2 diabetic rats",
"abstract": "<p>This study was performed to evaluate the therapeutic efficacy of exercise and oral melatonin on metabolic syndrome (MS), anxiety and depression-like behavior (ADB) in type 2 diabetes mellitus (T2DM) model in rat. Rats were allocated into five groups: non diabetes group, diabetes group, three treated group; diabetes rats disciplined with swimming exercise (40 min, 5 days per week) or oral melatonin (10 mg/kg bwt per day at 19.00 PM) alone or with combination. Exercise and oral melatonin significantly attenuated MS evidenced by improvement of hyperglycemia, insulin resistance, dyslipidemia, hyperleptinemia, and hypoadiponectinemia level in comparison with diabetes group. The ADB also markedly improved in exercise and oral melatonin treated rats as represented by decreased anxiety index, increased open arm entries and time spent in elevated plus maze, and reduced freezing behavior, increased entries and time spent in center in open field test. To know underlying molecular mechanisms, hippocampus tissue was analyzed. Interestingly, exercise combined with oral melatonin synergistically reduced serum corticosterone and hippocampus tissue level inflammatory cytokines and improved ATP level. Furthermore, this combination up-regulated the expression of brain-derived neurotrophic factor (BDNF), peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) and mitochondrial biogenesis related proteins, glucose transporter type 4 (GLUT4) in hippocampus tissue. Exercise and oral melatonin synergistically attenuated ADB in T2DM rats by attenuation of MS, neuroinflammation and normalizing corticosterone level via up-regulation of PGC-1α, mitochondrial biogenesis, BDNF, GLUT4, expression and ATP level. Thus, this treatment combination can be a promising tool in the management of MS, anxiety and depression in T2DM patients.</p>",
"DOI": "10.5455/jabet.2021.d124",
"views": 1212,
"downloads": 126,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/04/178-1619144787-Figure1.jpg",
"caption": "Figure 1. Anxiety-like effect of exercise and oral melatonin in the elevated plus-maze test (EPM) in type-2 diabetes mellitus rat. NC, normal control; DM, diabetes mellitus control group; DME. diabetic rat with exercise group; DMM. diabetic rat with melatonin supplemented group, DMME, diabetic rat with exercise and melatonin group. Data are presented as mean ± SE, *p<0.05 vs. control, and #p < 0.05 vs. DM.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/04/178-1619144787-Figure2.jpg",
"caption": "Figure 2. Antidepressant-like effect of exercise and oral melatonin in the open field test (OFT) in type-2 diabetes mellitus rat. NC, normal control; DM, diabetes mellitus control group; DME. diabetic rat with exercise group; DMM. diabetic rat with melatonin supplemented group, DMME, diabetic rat with exercise and melatonin group. Data are presented as mean ± SE, *p<0.05 vs. control, and #p < 0.05 vs. DM.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/04/178-1619144787-Figure3.jpg",
"caption": "Figure 3. Effect of exercise and oral melatonin on inflammatory cytokines and ATP in hippocampal tissue in type-2 diabetes mellitus rat. NC, normal control; DM, diabetes mellitus control group; DME. diabetic rat with exercise group; DMM. diabetic rat with melatonin supplemented group, DMME, diabetic rat with exercise and melatonin group. Data are presented as mean ± SE, *p<0.05 vs. control, and #p < 0.05 vs. DM.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/33/04/178-1619144787-Figure4.jpg",
"caption": "Figure 4. Effect of exercise and oral melatonin on the expression of proteins hippocampal tissue in type-2 diabetes mellitus rat. PGC-1 a, Peroxisome proliferator activated receptor gamma coactivator 1 a; NRF-1, nuclear respiratory factor 1; NRF-2, nuclear respiratory factor 2, mtTFA, mitochondrial transcription factor A, brain-derived neurotrophic factor (BDNF), glucose transporter type 4 (GLUT4). NC, normal control; DM, diabetes mellitus control group; DME. diabetic rat with exercise group; DMM. diabetic rat with melatonin supplemented group, DMME, diabetic rat with exercise and melatonin group. Data are presented as mean ± SE, *p<0.05 vs. control, and #p < 0.05 vs. DM.",
"featured": false
}
],
"authors": [
{
"id": 784,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd., Research Center, Incheon, Republic of Korea"
}
],
"first_name": "Md. Mahbubur",
"family_name": "Rahman",
"email": "mahbub@knotus.co.kr",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Mahbubur Rahman, PhD; KNOTUS Co., Ltd., Research Center, Incheon, Republic of Korea. Email: mahbub@knotus.co.kr",
"article": 184
},
{
"id": 785,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd., Research Center, Incheon, Republic of Korea"
},
{
"affiliation": "Lab of Hygienic Pharmacy, College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea"
}
],
"first_name": "Sung-Jin",
"family_name": "Park",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 184
},
{
"id": 786,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd., Research Center, Incheon, Republic of Korea"
},
{
"affiliation": "Lab of Hygienic Pharmacy, College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea"
}
],
"first_name": "Ha-Young",
"family_name": "Jeon",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 184
},
{
"id": 787,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd., Research Center, Incheon, Republic of Korea"
}
],
"first_name": "Sokho",
"family_name": "Kim",
"email": "skim@knotus.co.kr",
"author_order": 5,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sokho Kim, KNOTUS Co., Ltd., Research Center, Incheon, Republic of Korea. Email: skim@knotus.co.kr",
"article": 184
}
]
},
{
"id": 182,
"slug": "178-1619276772-lactoferrin-potential-functions-pharmacological-insights-and-therapeutic-promises",
"type": "review_article",
"manuscript_id": "178-1619276772",
"published": "2021-05-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/55/178-1619276772.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 223-237.",
"academic_editor": "Md Nabiul Islam, PhD; Yamaguchi University, Japan",
"cite_info": "Ahmed KA, Saikat ASM, et al. Lactoferrin: potential functions, pharmacological insights, and therapeutic promises. J Adv Biotechnol Exp Ther. 2021; 4(2): 223-237.",
"title": "Lactoferrin: potential functions, pharmacological insights, and therapeutic promises",
"abstract": "<p>Lactoferrin (LF) is an iron-binding multifunctional glycoprotein, act as a natural protective agent. In general, LF is involved in various physiological activities, including antibacterial, antifungal, antiviral, antiparasitic, anticarcinogenic and iron metabolism. The LF is most frequently found in milk as well as many other exocrine secretions such as saliva, bronchial mucus, seminal fluids, and gastro-intestinal fluids, respectively. Increased expression and secretion of LF may play a significant role in the first line of host defense. One of the primary functions of LF is to scavenge free iron in fluids and inflamed areas to avoid free radical-facilitated damage. LF influences the proliferation, maturation, and activity of immune cells at the cellular level. LF plays a significant protective role in inflammation, oxidative stress, fibrosis, endoplasmic reticulum (ER) stress, autophagy dysfunction, and mitochondrial dysfunction. Also, LF was found protective against various pathologies including anemia, sepsis, and diarrhoea in clinical settings. This article reviews the protective role of LF against different pathophysiological conditions and its therapeutic advances as well as further research prospects.</p>",
"DOI": "10.5455/jabet.2021.d123",
"views": 2330,
"downloads": 193,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/04/178-1619276772-Figure1.jpg",
"caption": "Figure 1. Functions of lactoferrin. This diagram depicts lactoferrin's diverse biological roles, which include iron metabolism, antiparasitic, anticarcinogenic, antiviral, antifungal, and antibacterial properties. Its ability to metabolize iron aids in maintaining homeostasis and preventing anemia, as well as providing other therapeutic benefits. Lactoferrin's antiparasitic properties have been linked to immune cell proliferation, which aids in parasitism prevention. Its anticarcinogenic effects inhibit carcinogenesis via several cancer pathway alterations. Lactoferrin has antiviral characteristics that resist such naked and coated DNA and/or RNA viruses of both animals and humans effectively. It has antifungal potential against a variety of fungi and is used as an antifungal therapy. Lactoferrin's bactericidal activity has demonstrated promising results against gram-positive and gram-negative bacteria, as well as inhibiting bacterial attachment.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/04/178-1619276772-Figure2.jpg",
"caption": "Figure 2. Pharmacological potentials of lactoferrin against pathological conditions. The diagram illustrates the complex pharmacological potentials of LF in relation to pathological disorders, including fibrosis, oxidative stress, endoplasmic reticulum (ER) stress, autophagy dysfunction, mitochondrial dysfunction, and inflammation. In renal fibrosis and liver fibrosis, LF has been shown to play an antifibrotic role. It maintains the physiological balance of ROS activity and protects cells from oxidative damage in a variety of pathological conditions. Lactoferrin reduces ER stress in ob/ob mice liver tissues. It has also been documented that it stimulates autophagy by activating several pathways in order to avoid autophagy dysfunction and apoptosis. Lactoferrin regulates the mitochondrial synthesis of H2O2 to protect the liver and other organs from mitochondrial dysfunctions. Its anti-inflammatory effects assist in the prevention of inflamed tissue inflammation as well as mediating host immunity.",
"featured": false
}
],
"authors": [
{
"id": 773,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, Bangladesh"
}
],
"first_name": "Kazi Ahsan",
"family_name": "Ahmed",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-4831-7758",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 182
},
{
"id": 774,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, Bangladesh"
}
],
"first_name": "Abu Saim Mohammad",
"family_name": "Saikat",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 182
},
{
"id": 775,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Akhi",
"family_name": "Moni",
"email": null,
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-5812-7755",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 182
},
{
"id": 776,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Sadia Akhter Mallik",
"family_name": "Kakon",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 182
},
{
"id": 777,
"affiliation": [
{
"affiliation": "Department of Genetics and Animal Breeding, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh"
}
],
"first_name": "Md. Rashedul",
"family_name": "Islam",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 182
},
{
"id": 778,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": "hasan800920@gmail.com",
"author_order": 6,
"ORCID": "https://orcid.org/0000-0003-2911-3255",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Jamal Uddin, PhD; Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of Korea. Email: hasan800920@gmail.com",
"article": 182
}
]
},
{
"id": 180,
"slug": "178-1615324379-hemostatic-activities-of-methanol-leaf-extracts-of-croton-megalocarpus-hutch-and-lantana-camara-linn",
"type": "original_article",
"manuscript_id": "178-1615324379",
"published": "2021-05-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/28/178-1615324379.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 215-222.",
"academic_editor": "Talha Bin Emran, PhD; BGC Trust University, Bangladesh",
"cite_info": "Muindi HM, Kibiti CM, et al. Hemostatic activities of methanol leaf extracts of Croton megalocarpus Hutch and Lantana camara Linn. J Adv Biotechnol Exp Ther. 2021; 4(2): 215-222.",
"title": "Hemostatic activities of methanol leaf extracts of Croton megalocarpus Hutch and Lantana camara Linn",
"abstract": "<p>The effects of <em>Croton megalocarpus</em> Hutch and <em>Lantana camara</em> Linn plants leave on blood coagulation has not been scientifically tested despite their common use in bleeding control. The current study investigates the effects of the plants’ leaves extracts on mice plasma coagulation time. Leaves of <em>C. megalocarpus</em> (H.) and <em>L. camara</em> (L.) were harvested, washed, air dried, crushed, extracted in absolute methanol and concentrated to dry powders. Preliminary qualitative phytochemical screen of both extracts was done. A total of 55 mice plasma samples were mixed with solutions of leaves extracts of both plants, before prothrombin time (PT) and activated partial thromboplastin time (aPTT) were determined. Data was analyzed using one – way ANOVA. Phytochemical screen on separate extracts of <em>C. megalocarpus</em> (H.) and <em>L. camara</em> (L.) revealed the presence of tannins, phenols, cardiac glycosides, and terpenoids. Moreover, <em>C. megalocarpus</em> (H.) extract contained steroids and flavonoids while that of <em>L. camara</em> (L.) had saponins. Solutions of the various concentrations of methanol leave extracts of <em>C. megalocarpus </em>(H.).<em> L. camara </em>(L.) and leaves blend extracts from both plants reduced aPTT and PT significantly (p<0.05) when mixed with normal mice plasma. Significant reduction of aPTT and PT of normal treated plasma was associated with the activity of tannins, phenols, flavonoids and saponins detected in the plant extracts.</p>",
"DOI": "10.5455/jabet.2021.d122",
"views": 1029,
"downloads": 141,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/04/178-1615324379-Figure1.jpg",
"caption": "Figure 1. Effects of leaves extracts on prothrombin time (PT). MECM (methanol leaves extract of Croton megalocarpus), MELC (methanol leaves extract of Lantana camara), MECM-MELC (methanol Croton megalocarpus and Lantana camara blended leaves extract). Bars with a different letter within individual clusters are significantly different (one-way ANOVA followed by Tukey post hock test p<0.05). Letters in order of increasing hemostatic activity: a, b, c, d.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/04/178-1615324379-Figure2.jpg",
"caption": "Figure 2. Comparison of effects leaves extracts on PT. MECM (methanol leaves extract of Croton megalocarpus), MELC (methanol leaves extract of Lantana camara), MECM-MELC (methanol Croton megalocarpus and Lantana camara blended leaves extract). Bars with a different letter within individual clusters are significantly different (one-way ANOVA followed by Tukey post hock test; p<0.05). Letters in order of increasing hemostatic activity: a, b.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/04/178-1615324379-Figure3.jpg",
"caption": "Figure 3. Comparison of effects of leaves extracts on aPTT. MECM (methanol leaves extract of Croton megalocarpus), MELC (methanol leaves extract of Lantana camara), MECM-MELC (methanol Croton megalocarpus and Lantana camara blended leaves extract). Bars with a different letter within individual clusters are significantly different (one-way ANOVA followed by Tukey post hock test p<0.05). Letters in order of increasing hemostatic activity: a, b, c, d.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/04/178-1615324379-Figure4.jpg",
"caption": "Figure 4. Comparison of effects of leaves extracts on aPTT. MECM (methanol leaves extract of Croton megalocarpus), MELC (methanol leaves extract of Lantana camara), MECM-MELC (methanol Croton megalocarpus and Lantana camara blended leaves extract). Bars with different letter within individual clusters are significantly different (one-way ANOVA followed by Tukey post hock test p<0.05). Letters in order of increasing hemostatic activity: a, b, c.",
"featured": false
}
],
"authors": [
{
"id": 764,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology & Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi-43844-00100, Kenya"
}
],
"first_name": "Hezron",
"family_name": "Muindi",
"email": "hezronmuindi@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Hezron Muindi, Department of Biochemistry, Microbiology & Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi-43844-00100, Kenya. Email: hezronmuindi@gmail.com",
"article": 180
},
{
"id": 765,
"affiliation": [
{
"affiliation": "Department of Pure and Applied Sciences, Technical University of Mombasa, Mombasa-90420-80100, Kenya"
}
],
"first_name": "Cromwell",
"family_name": "Kibiti",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 180
},
{
"id": 766,
"affiliation": [
{
"affiliation": "Department of Biochemistry, Microbiology & Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi-43844-00100, Kenya"
}
],
"first_name": "Mathew",
"family_name": "Ngugi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 180
}
]
},
{
"id": 179,
"slug": "178-1610465541-analysis-of-the-risk-of-cardiovascular-diseases-among-people-with-diabetes-according-to-triglyceride-level",
"type": "original_article",
"manuscript_id": "178-1610465541",
"published": "2021-04-03",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/02/178-1610465541.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 210-214.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea.",
"cite_info": "Obaidullah MM, Chowdhury MR, et al. Analysis of the risk of cardiovascular diseases among people with diabetes according to triglyceride level. J Adv Biotechnol Exp Ther. 2021; 4(2): 210-214.",
"title": "Analysis of the risk of cardiovascular diseases among people with diabetes according to triglyceride level",
"abstract": "<p>The low percentage of high-density lipoprotein-cholesterol (HDL-C) is strongly accompanied with cardiovascular diseases (CVD) where female are at higher risk than male and are the most dangerous reason of a disease called type 2 diabetes mellitus (T2DM). Although the relationship between gender-specific HDL-C / LDL-C level and T2DM is difficult to understand, however, it is suspected to be linked. To determine the HDL-C and LDL-C ratios among males and females with T2DM subjects, a study was conducted in the Department of Biochemistry Laboratory of BUHS general hospital, Mirpur, Dhaka, Bangladesh from July 2018 to November 2018 among 60 T2DM patients consisting of 30 males and 30 females. Study subjects with ages ranging from 35-65 were included in this study. Serum glucose and lipid profile was analyzed by enzymatic colorimetric method. Among the glycemic profile, the fasting serum glucose levels and 2 h after breakfast (ABF) glucose levels in male and female subjects were measured. The total serum cholesterol was significantly higher in the subject of females T2DM patient compared to male T2DM subjects (p=0.043). However, HDL-C and LDL-C did not show any significant difference between male and female T2DM subjects. In conclusion, our results show hypercholesterolemia among female T2DM subjects compared to male counterparts who might have higher risk of cardiovascular diseases.</p>",
"DOI": "10.5455/jabet.2021.d121",
"views": 867,
"downloads": 123,
"figures": [],
"authors": [
{
"id": 758,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Cell Biology, Bangladesh University of Health Sciences (BUHS), Mirpur, Dhaka 1216, Bangladesh"
}
],
"first_name": "Md. Mim",
"family_name": "Obaidullah",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 179
},
{
"id": 759,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Rayhan",
"family_name": "Chowdhury",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 179
},
{
"id": 760,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Shirmin",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 179
},
{
"id": 761,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Cell Biology, Bangladesh University of Health Sciences (BUHS), Mirpur, Dhaka 1216, Bangladesh"
}
],
"first_name": "Ashok Kumar",
"family_name": "Barmon",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 179
},
{
"id": 762,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Cell Biology, Bangladesh University of Health Sciences (BUHS), Mirpur, Dhaka 1216, Bangladesh"
}
],
"first_name": "Israt Ara",
"family_name": "Hossain",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 179
},
{
"id": 763,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Mohammad Nurul",
"family_name": "Matin",
"email": "nmatin@ru.ac.bd",
"author_order": 6,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Nurul Matin, PhD; Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi6205, Bangladesh. Email: nmatin@ru.ac.bd",
"article": 179
}
]
},
{
"id": 174,
"slug": "178-1613537836-characterization-of-human-articular-cartilage-derived-mesenchymal-progenitor-cells-from-osteoarthritis-patients",
"type": "original_article",
"manuscript_id": "178-1613537836",
"published": "2021-03-28",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/55/178-1613537836.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 200-209.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea.",
"cite_info": "Manjappa AB, Rao S, et al. Characterization of human articular cartilage derived mesenchymal progenitor cells from osteoarthritis patients. J Adv Biotechnol Exp Ther. 2021; 4(2): 200-209.",
"title": "Characterization of human articular cartilage derived mesenchymal progenitor cells from osteoarthritis patients",
"abstract": "<p>Osteoarthritis (OA) is a leading pathological condition resulting in the degeneration and destruction of articular cartilage. The presence of inherent mesenchymal progenitor cells (MPCs) within the articular cartilage has led to explore the possible reparative mechanisms to regenerate and restore the functional and mechanical properties of hyaline cartilage. The present <em>in vitro</em> study was aimed to identify and characterize the MPCs derived from OA cartilage. MPCs derived from the explant culture of OA cartilage were analyzed in terms of cellular and biological properties, and multilineage differentiation abilities. Upon cell surface marker analysis, MPCs were CD73+, CD90+, CD166+, CD146-, CD34-, CD45-, and HLA-DR-, whose expression defines stemness and chondroprogenitor status. MPCs exhibited a higher proliferative index and limited or no senescence activity till later passages. Trilineage differentiation towards osteogenesis, adipogenesis, and chondrogenesis was observed with cytochemical staining and also by mRNA expression of lineage-specific markers by RT-qPCR. The results showed that OA cartilage harbors a viable pool of MPCs with greater chondrogenic potential. These cell niches could serve as a superior cell source for cartilage regeneration due to their committed progeny and hence could prevent heterotypic cartilage formation.</p>",
"DOI": "10.5455/jabet.2021.d120",
"views": 609,
"downloads": 80,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1613537836-Figure1.jpg",
"caption": "Figure 1. Morphology of osteoarthritis (OA) cartilage-derived mesenchymal progenitor cells (MPCs). (A) MPCS exhibited their early release from tissue explants and firm attachment onto the cell culture dish at passage 0 (P0). (B) The adherent cells formed a relatively homogeneous population and showed a similar fibroblast-like (short to elongated spindle) morphology. Images (4x).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1613537836-Figure2.jpg",
"caption": "Figure 2. Viability and growth kinetics of MPCs. (A & B) The viability of MPCs at different passages and various time points of passage 2. The mean percentage values are plotted. (C) The proliferation rate of MPCs was determined by counting the cells at different time intervals, such as day 0, 3, 6, 9, and 12. (D) The growth potential of MPCs is presented as PDT in hours. Superscripts a, b and c indicate statistically significant differences at P<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1613537836-Figure3.jpg",
"caption": "Figure 3. Senescence associated β-Galactosidase Staining Kit (SA-β-GAL) and colony-forming unit (CFU) assay of MPCs. (A) SA-β-Gal activity of MPCs. Images (10x). (B) MPCs displayed crystal violet stained colonies on the 14th day. Both macro and microphotographs indicate the clonogenic potential. Image: 4x (C) The mean percentage of colonies formed by MPCs. Superscripts a, b and c indicate statistically significant differences at P<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1613537836-Figure4.jpg",
"caption": "Figure 4. Alkaline phosphatase activity (ALP) of MPCs. (A&B) MPCs exhibited ALP activity in the 11th-day cultures. Purple staining was observed in both non-induced and osteogenesis-induced MPCs. Both macro and microphotographs indicated evident BCIP/NBT staining in Induced MPCs for obvious reasons. Images (4x). (C) ALP activity of MPCs was quantified by RT-qPCR. A significantly higher mRNA expression was observed in the induced MPCs (OI-MPCs) than the non-induced MPCs (Control-MPCs). Superscripts a and b indicate statistically significant differences at P<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1613537836-Figure5.jpg",
"caption": "Figure 5. Representative images of immuno-phenotyping analysis of MPCs by flow cytometry. MPCs displayed positive staining for markers, such as CD73, CD90, CD166 and negative staining for CD146, CD34, CD45 and HLA-DR. Red and green filled histograms show isotype control and expression of indicated markers, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1613537836-Figure6.jpg",
"caption": "Figure 6. Osteogenesis of MPCs. (A) MPCs were capable of differentiating into osteocytes upon induction for 28 days as confirmed by Alizarin red staining, which stained calcium deposits. Inset: Macroscopic image. No staining was observed in control. Images: 4x. (B) Relative mRNA expression levels of genes RUNX2, OPN, and ON analyzed by RT-qPCR. Superscripts a, b, c indicate statistically significant difference amongst the MPCs at P<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1613537836-Figure7.jpg",
"caption": "Figure 7. Adipogenesis of MPCs. (A) Adipogenesis-induced MPCs were capable of forming lipid vacuoles as indicated by oil red O staining. No staining was observed in control. Images: 4x. (B) Relative mRNA expression levels of genes LPL, AP2, and PPAR-γ analyzed by RT-qPCR. Superscripts a and b indicate statistically significant difference amongst the MPCs at P<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1613537836-Figure8.jpg",
"caption": "Figure 8. Chondrogenesis of MPCs. (A) Chondrogenic induction of MPCs for 28 days resulted in the formation of characteristic spheroids, demonstrated by Alcian blue staining. Inset: Macroscopic image. No staining was observed in control. Images: 4x. (B) Relative mRNA expression levels of genes ACAN, COL2-α1, SOX9, TGF-β3 and VIM analyzed by RT-qPCR. Superscripts a and b indicate statistically significant difference amongst the MPCs at P<0.05.",
"featured": false
}
],
"authors": [
{
"id": 740,
"affiliation": [
{
"affiliation": "Nitte University Centre for Stem Cell Research and Regenerative Medicine, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India"
}
],
"first_name": "Akshay Bairapura",
"family_name": "Manjappa",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 174
},
{
"id": 741,
"affiliation": [
{
"affiliation": "Nitte University Centre for Stem Cell Research and Regenerative Medicine, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India"
}
],
"first_name": "Shama",
"family_name": "Rao",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 174
},
{
"id": 742,
"affiliation": [
{
"affiliation": "Nitte University Centre for Stem Cell Research and Regenerative Medicine, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India"
},
{
"affiliation": "Department of Orthopaedics, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India"
}
],
"first_name": "Siddharth",
"family_name": "Shetty",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 174
},
{
"id": 743,
"affiliation": [
{
"affiliation": "Nitte University Centre for Stem Cell Research and Regenerative Medicine, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India"
}
],
"first_name": "Veena",
"family_name": "Shetty",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 174
},
{
"id": 744,
"affiliation": [
{
"affiliation": "Nitte University Centre for Stem Cell Research and Regenerative Medicine, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India"
},
{
"affiliation": "Canterbury Christ Church University, Faculty of Health and Wellbeing, Kent, United Kingdom"
}
],
"first_name": "Ananthram Shetty",
"family_name": "Asode",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 174
},
{
"id": 745,
"affiliation": [
{
"affiliation": "Nitte University Centre for Stem Cell Research and Regenerative Medicine, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India"
},
{
"affiliation": "Department of Orthopaedics, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India"
}
],
"first_name": "Shantharam Shetty",
"family_name": "Molahalli",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 174
},
{
"id": 749,
"affiliation": [
{
"affiliation": "Nitte University Centre for Stem Cell Research and Regenerative Medicine, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India"
}
],
"first_name": "Mohana Kumar",
"family_name": "Basavarajappa",
"email": "mohanakumar@nitte.edu.in",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohana Kumar Basavarajappa, PhD; Nitte University Centre for Stem Cell Research and Regenerative Medicine, KS Hegde Medical Academy, Nitte Deemed to be University, Deralakatte-575018, Mangaluru, India. Email: mohanakumar@nitte.edu.in",
"article": 174
}
]
},
{
"id": 172,
"slug": "178-1611907708-present-scenario-of-covid-19-in-bangladesh-and-government-preparedness-for-facing-challenges",
"type": "review_article",
"manuscript_id": "178-1611907708",
"published": "2021-03-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/36/178-1611907708.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 187-199.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Rahman MR, Sajib EH, et al. Present scenario of COVID-19 in Bangladesh and government preparedness for facing challenges. J Adv Biotechnol Exp Ther. 2021; 4(2): 187-199.",
"title": "Present scenario of COVID-19 in Bangladesh and government preparedness for facing challenges",
"abstract": "<p>The COVID-19 pandemic caused by SARS-CoV-2 has been showing a speedy growth in the number of infected patients with a remarkable mortality rate, thus it has become a worldwide public health concern. From March 8, 2020, the virus started spreading in Bangladesh. Since then, people got infected so exponentially that the country positions at the list of top infected countries in the world. Therefore, the objective of this comprehensive review was representing the overall scenario of COVID-19 in different sectors of Bangladesh. Because of insufficient scientific publications, we chose materials published by several agencies, media outlets, newspapers, and policy experts to retrieve details information. Up to 4 March 2021, 547,930 confirmed cases and 8,428 deaths were reported in Bangladesh. An alarming fact is that while the global mutation rate of coronavirus is 7.23 % in average, the rate is 12.6 % in Bangladesh. Although the government ruled preventive strategies such as nationwide lockdown, social distancing, contact monitoring, quarantine, and isolation, it was difficult to implement those due to lack of public awareness, inappropriate attitudes and so on. Moreover, the overburdened healthcare system had a weak response at initial stage because of insufficient healthcare facilities. Consequently, this pandemic affected severely almost all the important sectors of the country, specifically the economy, agriculture, education, and health sector. Hence, focusing on healthcare system, maintaining social distance, and other essential precautions can limit the spread of infection and help to alleviate the severity of this pandemic.</p>",
"DOI": "10.5455/jabet.2021.d119",
"views": 2106,
"downloads": 145,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/34/04/178-1611907708-Figure1.jpg",
"caption": "Figure 1. District-wise scenario of COVID-19 confirmed cases in Bangladesh up to 15 December 2020. The gradient of yellow to orange is representing the number of confirmed cases per 100,000 people in each district classified into five classes and the circle is representing the total confirmed cases per district. The data of confirmed cases was adopted from IEDCR (https://iedcr.gov.bd) and the data of population for each district was adopted from the Bangladesh Bureau of Statistics (BBS).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/34/04/178-1611907708-Figure2.jpg",
"caption": "Figure 2. Monthly COVID-19 tests and positive cases in Bangladesh up to 28 February 2021 (Available from https://iedcr.gov.bd).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/34/04/178-1611907708-Figure3.jpg",
"caption": "Figure 3. Distribution of number of deaths per month (from 08 March to 28 February) caused by COVID-19 (Available from https://iedcr.gov.bd).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/34/04/178-1611907708-Figure4.jpg",
"caption": "Figure 4. Distribution of number of recoveries per month (from 08 March to 28 February) caused by COVID-19 (Available from https://iedcr.gov.bd).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/34/04/178-1611907708-Figure5.jpg",
"caption": "Figure 5. Graphical representation on COVID-19 situation in Bangladesh, public response to this pandemic, challenges faced by the people, and government in this pandemic and the initiatives taken by the government to mitigate the situation.",
"featured": false
}
],
"authors": [
{
"id": 734,
"affiliation": [
{
"affiliation": "Department of Molecular Biology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Mohammad Rejaur",
"family_name": "Rahman",
"email": "rejaur.mge@sau.ac.bd",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Rejaur Rahman, Department of Molecular Biology and Genetic Engineering, Sylhet Agricultural University, Sylhet3100, Bangladesh. Email: rejaur.mge@sau.ac.bd",
"article": 172
},
{
"id": 735,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology & Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Emran Hossain",
"family_name": "Sajib",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 172
},
{
"id": 736,
"affiliation": [
{
"affiliation": "Department of Molecular Biology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Ishtiak Malique",
"family_name": "Chowdhury",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 172
},
{
"id": 737,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology & Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Anik",
"family_name": "Banik",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 172
},
{
"id": 738,
"affiliation": [
{
"affiliation": "Department of Basic Science and Language, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Rahul",
"family_name": "Bhattacharya",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 172
},
{
"id": 739,
"affiliation": [
{
"affiliation": "Department of Forestry and Environmental Science, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Hasan",
"family_name": "Ahmed",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 172
}
]
},
{
"id": 168,
"slug": "178-1610280511-antibacterial-activity-of-silver-nanoparticles-synthesized-from-leaf-and-flower-extracts-of-galinsoga-formosa",
"type": "original_article",
"manuscript_id": "178-1610280511",
"published": "2021-03-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1610280511.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 178-186.",
"academic_editor": "Hasan-Al-Faruque, PhD; DGIST, South Korea",
"cite_info": "Mahmod MR, Junayed A, et al. Antibacterial activity of silver nanoparticles synthesized from leaf and flower extracts of Galinsoga formosa. J Adv Biotechnol Exp Ther. 2021; 4(2): 178-186.",
"title": "Antibacterial activity of silver nanoparticles synthesized from leaf and flower extracts of Galinsoga formosa",
"abstract": "<p>Silver Nanoparticles (Ag-NPs) are progressively exercised as an antimicrobial agent among myriad applications. The rapid emergence of microbial resistance to conventional antibiotics by multidrug-resistant pathogens has become a threat to the global health community. Traditionally used herbal plants are good sources of bioactive phytochemicals and <em>Galinsoga formosa</em> is one of them. Thus, Ag-NPs are biologically synthesized, intending to evaluate the antibacterial activity of <em>G. formosa. </em>Disc diffusion assay was used to assess the antibacterial activity of leaf and flower crude extracts distinctly as well as of green synthesized Ag-NPs <em>in vitro</em>. The biosynthesis of Ag-NPs was primarily confirmed by brownish color solution and later by UV-visible spectrophotometer. Ag-NPs synthesized from <em>G. formosa </em>leaf, and flower extract showed antibacterial activity against gram-positive (<em>S. aureus, S. mutans,</em> and <em>S. epidermidis</em>) and gram-negative (<em>K. pneumoniae </em>and <em>P. aeruginosa</em>) bacteria, where gram-negative bacteria were more sensitive than gram-positive bacteria. The highest zone of inhibition was observed against <em>P. aeruginosa</em> (13.33±0.58 mm) by applying Ag-NPs synthesized from <em>G. formosa </em>flower extract. In contrast, the lowest zone of inhibition was observed against <em>S. epidermidis</em> (6.33±0.58 mm). Antibacterial activity of Ag-NPs from flower and leaf extracts was considerably higher as compared to their respective crude extract. Further, Ag-NPs from the flower extract was exhibited more growth inhibitory response than the leaf extract. Hence, the findings of this research suggested that the synthesized Ag-NPs from <em>G. formosa </em>leaf and flower extract were exhibited antibacterial activity. Such synthesized Ag-NPs might help to develop new drug for combating against various diseases.</p>",
"DOI": "10.5455/jabet.2021.d118",
"views": 1618,
"downloads": 196,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/04/178-1610280511-Figure1.jpg",
"caption": "Figure 1. Overview of the antibacterial activity of synthesized Ag-NPs from G. Formosa.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/04/178-1610280511-Figure2.jpg",
"caption": "Figure 2. Confirmation of synthesized Ag-NPs of the leaf (A) and flower (B) from G. formosa by UV-visible Spectrophotometer.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/04/178-1610280511-Figure3.jpg",
"caption": "Figure 3. Comparative illustration of the activity of leaf and flower extract at 10 mM AgNO3¬ (A) vs 5mM AgNO3 (B), and gram-negative vs gram-positive bacteria.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/04/178-1610280511-Figure4.jpg",
"caption": "Figure 4a. Antibacterial activity of Ag-NPs synthesized from G. formosa leaf extract against S. aureus (P-1), K. pneumoniae (P-2), S. mutans (P-3), S. epidermidis (P-4), and P. aeruginosa (P-5). Ag-NPs using 10 mM (A) and 5 mM (B) AgNO3 respectively, a disc with deionized water as a negative control (C), and ampicillin disc (D).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/04/178-1610280511-Figure5.jpg",
"caption": "Figure 4b. Antibacterial activity of Ag-NPs synthesized from G. formosa flower extract against P. aeruginosa (P-6). S. aureus (P-7), S. mutans (P-8), S. epidermidis (P-9), and K. pneumoniae (P-10). Ag-NPs using 10 mM (A) and 5 mM (B) AgNO3 respectively, a disc with deionized water as a negative control (C), and ampicillin disc (D).",
"featured": false
}
],
"authors": [
{
"id": 708,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Md. Rashel",
"family_name": "Mahmod",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 709,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Asadullah",
"family_name": "Junayed",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 710,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Chaity",
"family_name": "Bhowmick",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 711,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Sagarika Adhikary",
"family_name": "Sompa",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 712,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Tania",
"family_name": "Sultana",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 713,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Tanjina",
"family_name": "Akter",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 714,
"affiliation": [
{
"affiliation": "Department of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail -1902, Bangladesh"
}
],
"first_name": "Md. Zainul",
"family_name": "Abedin",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 715,
"affiliation": [
{
"affiliation": "Department of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail -1902, Bangladesh"
}
],
"first_name": "Md. Abu",
"family_name": "Zubair",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 716,
"affiliation": [
{
"affiliation": "Department of Microbiology, Jagannath University, Dhaka-1100, Bangladesh"
}
],
"first_name": "Mohammad Ariful",
"family_name": "Islam",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 717,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Md. Roman",
"family_name": "Mogal",
"email": null,
"author_order": 10,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 718,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Marina",
"family_name": "Khatun",
"email": null,
"author_order": 11,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 168
},
{
"id": 719,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh"
}
],
"first_name": "Md. Asaduzzaman",
"family_name": "Sikder",
"email": "sikderbmb@gmail.com",
"author_order": 12,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Asaduzzaman Sikder, Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail -1902, Bangladesh. Email: sikderbmb@gmail.com",
"article": 168
}
]
},
{
"id": 165,
"slug": "178-1610833489-a-molecular-phylogeny-of-taeniophyllum-thrj-inferred-from-dna-barcode-regions",
"type": "original_article",
"manuscript_id": "178-1610833489",
"published": "2021-03-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/06/178-1610833489.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 171-177.",
"academic_editor": "Shahed Uddin Ahmed Shazib, PhD; Smith College, USA",
"cite_info": "Tallei TE, Pelealu JJ, et al. A molecular phylogeny of Taeniophyllum THRJ inferred from DNA barcode regions. J Adv Biotechnol Exp Ther. 2021; 4(2): 171-177.",
"title": "A molecular phylogeny of Taeniophyllum THRJ inferred from DNA barcode regions",
"abstract": "<p><em>Taeniophyllum</em> is one of the smallest orchids in the world. This genus has around 210 species listed in the plant list. One type of this orchid is found in the Juanda Forest Park (THRJ) Bandung. This orchid is difficult to identify at the species level due to its limited morphological character and very small size. The taxonomy and conservation status of this plant are unknown; therefore, it can be used as a model for developing DNA barcodes for this genus. In this study, two DNA barcode markers (<em>mat</em>K and ITS) were used to reconstruct the phylogenetic relationship of <em>Taeniophyllum</em> THRJ. By using <em>mat</em>K sequences, <em>Taeniophyllum</em> THRJ was grouped with <em>Taeniophyllum glandulosum</em> and <em>T. aphyllum</em>. However, using the ITS sequence, <em>Taeniophyllum</em> THRJ was positioned together with <em>Microtatorchis</em> sp., <em>T. smithii</em>, and <em>T. complanatum</em>. The results showed that the <em>mat</em>K gene can be used for DNA barcoding of <em>Taeniophyllum </em>orchids. The use of the ITS sequence for the <em>Taeniophyllum </em>group still cannot be confirmed yet. Based on <em>mat</em>K sequences and possibly ITS sequences, it can be concluded that <em>Taeniophyllum </em>THRJ is not <em>T. glandulosum</em> but is another species of <em>Taeniophyllum</em>.</p>",
"DOI": "10.5455/jabet.2021.d117",
"views": 1218,
"downloads": 130,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1610833489-Figure1.jpg",
"caption": "Figure 1. Root and flowers of Taeniophyllum THRJ.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1610833489-Figure2.jpg",
"caption": "Figure 2. The DNA barcode regions of rbcL and matK genes, and ITS which were successfully amplified.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1610833489-Figure3.jpg",
"caption": "Figure 3. Phylogenetic tree reconstruction of Taeniophyllum THRJ of matK gene (assigned as matK Taeniphyllum) using maximum likelihood method. Grouping by similarity to sequences is indicated. Proportions of bootstrap are indicated near the nodes.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/04/178-1610833489-Figure4.jpg",
"caption": "Figure 4. Phylogenetic tree reconstruction of Taeniophyllum THRJ ITS sequence using maximum likelihood. Grouping by similarity to sequences is indicated. Proportions of bootstrap are indicated near the nodes.",
"featured": false
}
],
"authors": [
{
"id": 692,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Trina Ekawati",
"family_name": "Tallei",
"email": "trina_tallei@unsrat.ac.id",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Prof. Dr. Trina E. Tallei, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sam Ratulangi, Manado, North Sulawesi-95115, Indonesia, Email: trina_tallei@unsrat.ac.id",
"article": 165
},
{
"id": 695,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Johanis Jullian",
"family_name": "Pelealu",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 165
},
{
"id": 696,
"affiliation": [
{
"affiliation": "Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia"
}
],
"first_name": "Beivy Jonathan",
"family_name": "Kolonam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 165
},
{
"id": 697,
"affiliation": [
{
"affiliation": "Balai Taman Hutan Raya Ir. H. Juanda Bandung. Kompleks Tahura, Jl. Ir. H. Djuanda No.99, Ciburial, Cimenyan, Bandung 40198, West Java, Indonesia"
}
],
"first_name": "Lianda",
"family_name": "Lubis",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 165
},
{
"id": 698,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Shafi",
"family_name": "Mahmud",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 165
},
{
"id": 699,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh"
}
],
"first_name": "Talha Bin Emran",
"family_name": "Emran",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran, PhD; Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh. Email: talhabmb@bgctub.ac.bd",
"article": 165
}
]
},
{
"id": 163,
"slug": "178-1611767486-methanolic-extract-of-moringa-oleifera-leaves-mediates-anticancer-activities-through-inhibiting-nf-b-and-enhancing-ros-in-ehrlich-ascites-carcinoma-cells-in-mice",
"type": "original_article",
"manuscript_id": "178-1611767486",
"published": "2021-03-15",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/17/178-1611767486.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 161-170.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea.",
"cite_info": "Das PK, Asha YS, et al. Methanolic extract of Moringa oleifera leaves mediates anticancer activities through inhibiting NF-𝜅B and enhancing ROS in Ehrlich ascites carcinoma cells in mice. J Adv Biotechnol Exp Ther. 2021; 4(2): 161-170.",
"title": "Methanolic extract of Moringa oleifera leaves mediates anticancer activities through inhibiting NF-𝜅B and enhancing ROS in Ehrlich ascites carcinoma cells in mice",
"abstract": "<p>Cancer is one of the vital causes of morbidity and mortality worldwide and recognized as the second most leading cause of death. Considering the side effects and high cost of synthetic anticancer drugs, plant might act as a source of novel anticancer agents with better host safety. In this investigation, Moringa oleifera leaves methanolic extract (MOLME) was subjected to evaluate its anti-proliferative effect with molecular mechanism in Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice. MOLME inhibited EAC cell growth approximately 64% at the dose of 200mg/kg body weight. DAPI stained cells had shown nuclear condensation and fragmentation in treated cells. Up-regulation of pro-apoptotic gene, <em>Bax</em> and tumor suppressor gene, <em>p53</em>; and down-regulation of anti-apoptotic gene <em>Bcl-2</em> confirm the induction of EAC cells followed by MOLME treatment. Moreover, MOLME inhibited NF-𝜅B activity and enhanced ROS generation. Results suggest that the anticancer effect of MOLME is associated with the induction of apoptosis through activation of p53 and reciprocal expression of Bax and Bcl-2. The apoptosis might be triggered by the inactivation of NF-𝜅B and generation of ROS. GC-MS analysis revealed that MOLME contains several bioactive compounds, thus it is assumed that the anticancer properties could be attributed by the active leads and could be potential resource of chemotherapeutics.</p>",
"DOI": "10.5455/jabet.2021.d116",
"views": 808,
"downloads": 125,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/05/178-1611767486-Figure1.jpg",
"caption": "Figure 1. Tumor weight of EAC bearing mice treated with MOLME and bleomycin. Results are shown as mean ± SD (standard deviation) (n=4). Mean with different lowercase letters are significantly different at P < 0.05 by Duncan’s multiple-range test.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/05/178-1611767486-Figure2.jpg",
"caption": "Figure 2. Effects of MOLME on EAC cell growth inhibition. Data were expressed as mean ± SD (n=4). Mean with different lowercase letters are significantly different at P < 0.05 by Duncan’s multiple-range test.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/05/178-1611767486-Figure3.jpg",
"caption": "Figure 3. DAPI staining of EAC cell. (A) represents fluorescence and optical microscopic of untreated EAC cells, whereas (B) represents MOLME treated cells. Under microscope, normal and round shape nucleuses as well as cells appeared where cells from MOLME treated mice showed the nucleuses and cells having apoptotic properties which are indicated by arrows.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/05/178-1611767486-Figure4.jpg",
"caption": "Figure 4. Induction of apoptosis and Inactivation of NF-𝜅B in EAC cells. Amplification of cell proliferation related genes p53, Bax, Bcl-2, and NF-𝜅B and control gene GAPDH. PCR reaction products separated on agarose gel stained with ethidium bromide. T RNA from EAC cells of MOLME treated mice and C RNA from EAC cells of MOLME untreated mice. (a) Expression of GAPDH in case of both control and treated EAC cells, (b) Upregulation of p53 gene in treated cells in compare to control, (c) Upregulation of Bax gene in treated cells in compare to control, (d) Downregulation of Bcl-2 gene in case of treatment compared to control, and (e) Down-regulation of NF-𝜅B gene in treated cells compared to untreated control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/05/178-1611767486-Figure5.jpg",
"caption": "Figure 5. Effects of MOLME in intracellular ROS generation in EAC cells. Observation of cells by fluorescence microscope after incubation with DCFH-DA. (A) Control EAC cells, (B) MOLME treated cells.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/05/178-1611767486-Figure6.jpg",
"caption": "Figure 6. GC/MS chromatogram of MOLME.",
"featured": false
}
],
"authors": [
{
"id": 681,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Plabon Kumar",
"family_name": "Das",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 163
},
{
"id": 682,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Saharia Yeasmin",
"family_name": "Asha",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 163
},
{
"id": 683,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Mst. Ayesha",
"family_name": "Siddika",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 163
},
{
"id": 684,
"affiliation": [
{
"affiliation": "Institute of Tissue Banking and Biomaterial Research, Atomic Energy Research Establishment, Gonakbari, Savar, Dhaka-1349, Bangladesh"
}
],
"first_name": "Ayesha",
"family_name": "Siddika",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 163
},
{
"id": 685,
"affiliation": [
{
"affiliation": "Environmental and Organic Laboratory, Atomic Energy Centre, Dhaka, Bangladesh"
}
],
"first_name": "A. R. M",
"family_name": "Tareq",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 163
},
{
"id": 686,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Farhadul",
"family_name": "Islam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 163
},
{
"id": 687,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Jahan Ara",
"family_name": "Khanam",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 163
},
{
"id": 688,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Md. Abdur",
"family_name": "Rakib",
"email": "mar@ru.ac.bd",
"author_order": 8,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Abdur Rakib, PhD; Associate Professor, Department of\r\nBiochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh. Email: mar@ru.ac.bd",
"article": 163
}
]
},
{
"id": 153,
"slug": "178-1612493625-follow-up-of-bacterial-and-physicochemical-quality-of-water-during-live-transportation-of-climbing-perch-anabas-testudineus-in-bangladesh",
"type": "original_article",
"manuscript_id": "178-1612493625",
"published": "2021-03-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/11/178-1612493625.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 149-160.",
"academic_editor": "Md. Niamul Haque, PhD; Incheon National University, South Korea",
"cite_info": "Hossain MM, Bhuiyan ANMRK, et al. Follow-up of bacterial and physicochemical quality of water during live transportation of Climbing perch (Anabas testudineus) in Bangladesh. J Adv Biotechnol Exp Ther. 2021; 4(2): 149-160.",
"title": "Follow-up of bacterial and physicochemical quality of water during live transportation of Climbing perch (Anabas testudineus) in Bangladesh",
"abstract": "<p>This study was conducted to evaluate the changes in viable count of bacteria and physicochemical parameters, and their correlations in the changing pattern during live transportation of Climbing perch, (<em>Anabas testudineus</em>). Investigations were conducted in three live fish supply channels of Bangladesh from Mymensingh to Dhaka (channel 1), Mymensingh to Sylhet (channel 2), and from Mymensingh to Rajshahi (channel 3). It took nearly 6 h in channel 1, 8 h in channel 2, and 8 h in channel 3 for the live fish to reach the unloading points of destination. Viable count of bacteria and physicochemical parameters, such as temperature, dissolved oxygen (DO), pH, and ammonia were recorded from 0 hr with 2 h interval during transportation. The viable count of bacteria in water increased several folds from the initial values and showed a regrowth within the system. In all the supply channels, water temperature and pH were more or less stable with the headway of the transportation, but remarkable differences were observed in the concentrations of DO and ammonia. Although the initial (0 hr) DO level was varying among the supply channels, a decreasing trend was observed at the end in every channel. On the other hand, ammonia concentration was gradually increased during the transportation process. Thus, a gradual decline in water quality (decreased DO level, increased ammonia concentration, and higher bacterial regrowth) may negatively affect the survival and quality of live fish during transportation.</p>",
"DOI": "10.5455/jabet.2021.d115",
"views": 1089,
"downloads": 81,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/37/05/178-1612493625-Figure1.jpg",
"caption": "Figure 1. Map showing location of sampling supply channels of live transportation of Climbing perch (Anabas testudineus) in Bangladesh (channel 1: Muktagacha, Mymensingh to Showarighat, Dhaka; channel 2: Muktagacha, Mymensingh to Poschim kazir Bazar, Sylhet; channel 3: Muktagacha, Mymensingh to Saheb Bazar, Rajshahi). Images were extracted from DIVA-GIS using Geographical Information System (GIS). The map was developed by using ArcMap version 10.7.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/37/05/178-1612493625-Figure2.jpg",
"caption": "Figure 2. Changes of physicochemical parameters along the fish supply channels of live transportation of Climbing perch (Anabas testudineus) in Bangladesh. There were no statistically significant differences in the parameters from the studied supply channles (p= 0.97, two-factor ANOVA without replication).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/37/05/178-1612493625-Figure3.jpg",
"caption": "Figure 3. Changes in viable cell count of bacteria in water along the fish supply channels of live transportation of Climbing perch (Anabas testudineus) in Bangladesh. There were no statistically significant differences in the viable counts from the studied supply channels (p= 0.4, two-factor ANOVA without replication).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/37/05/178-1612493625-Figure4.jpg",
"caption": "Figure 4. Correlation between (A) viable cell count of bacteria and ammonia, (B) viable cell count of bacteria and dissolved oxygen in supply channel 1 during live transportation of Climbing perch (Anabas testudineus) in Bangladesh.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/37/05/178-1612493625-Figure5.jpg",
"caption": "Figure 5. Correlation between (A) viable cell count of bacteria and ammonia, (B) viable cell count of bacteria and dissolved oxygen in supply channel 2 during live transportation of Climbing Perch (Anabas testudineus) in Bangladesh.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/37/05/178-1612493625-Figure6.jpg",
"caption": "Figure 6. Correlation between (A) viable cell count of bacteria and ammonia, (B) viable cell count of bacteria and dissolved oxygen in supply channel 3 during live transportation of Climbing perch (Anabas testudineus) in Bangladesh.",
"featured": false
}
],
"authors": [
{
"id": 641,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Mubarack",
"family_name": "Hossain",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 153
},
{
"id": 645,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "A N M Rezvi Kaysar",
"family_name": "Bhuiyan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 153
},
{
"id": 646,
"affiliation": [
{
"affiliation": "Technical-Fisher, Biswas Agro Fisheries and Hatchery Ltd., Mahbub Group of Industries, Trisal, Mymensingh, Bangladesh"
}
],
"first_name": "Md. Anwar",
"family_name": "Hossain",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 153
},
{
"id": 647,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Naim",
"family_name": "Uddin",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 153
},
{
"id": 648,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Ismail",
"family_name": "Hossain",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 153
},
{
"id": 649,
"affiliation": [
{
"affiliation": "Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
},
{
"affiliation": "Atmosphere and Ocean Research Institute, The University of Tokyo, Chiba 277-8564, Japan"
}
],
"first_name": "Md. Nurul",
"family_name": "Haider",
"email": "raselmnh@bau.edu.bd",
"author_order": 6,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Nurul Haider, PhD; Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, Email: raselmnh@bau.edu.bd",
"article": 153
}
]
},
{
"id": 152,
"slug": "178-1611554456-potential-roles-of-vitamin-d-in-the-treatment-of-covid-19-patient-and-improving-maternal-and-child-health-during-pandemic",
"type": "review_article",
"manuscript_id": "178-1611554456",
"published": "2021-03-04",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/19/178-1611554456.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 133-148.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Ahmed N, Araf Y, et al. Potential roles of vitamin D in the treatment of COVID-19 patient and improving maternal and child health during pandemic. J Adv Biotechnol Exp Ther. 2021; 4(2): 133-148.",
"title": "Potential roles of vitamin D in the treatment of COVID-19 patient and improving maternal and child health during pandemic",
"abstract": "<p>The coronavirus disease 2019 (COVID-19) pandemic is supposed to cause Vitamin D deficiency in many people by a direct effect of home quarantine in the affected countries. Generally, vitamin D provides the human body with significant health benefits including bone development, specific gene regulation, and protection against different diseases. However, deficiency of the optimal amount of vitamin D inside the human body may result in susceptibility to multiple infectious diseases. Therefore, with vitamin D levels gravely decreased by reduced movement and activity, a number of possible negative outcomes are expected in COVID-19 patients, pregnant women, and children during this ongoing pandemic. Vitamin D has a direct inhibitory effect on post-infection through a number of mechanisms that promise to make vitamin D a future adjunctive therapy for COVID-19 treatment. Besides, clinical evidence also supports its role in preventing pregnancy complications and improving pregnancy outcomes. Consistent with the manifold role of vitamin D, an increasing number of studies suggest its role in improving the mental health of children who have been adversely affected throughout this pandemic. This review article discusses the potential roles of vitamin D on COVID-19 patients, pregnant women, and children focusing its scope to become a supplementary candidate for these vulnerable groups to combat the ongoing pandemic.</p>",
"DOI": "10.5455/jabet.2021.d114",
"views": 1997,
"downloads": 161,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/05/178-1611554456-Figure1.jpg",
"caption": "Figure 1. The metabolic pathway of Vitamin D. In the skin, 7- dehydrocholesterol absorbs UVB and is converted to pre-vitamin D3. Pre-vitamin D3 then rapidly transforms into vitamin D3. Dietary vitamin D can be in the form of vitamin D2 (animal source) and vitamin D3 (plant source). The vitamin D is hydroxylated once in the liver and a second time in the kidney. The active metabolite, calcitriol, then travels in blood with the help of vitamin D binding protein to target cells. UVB: Ultraviolet B, VDR: vitamin D Receptor, CYP2R1: Cytochrome P450 Family 2 Subfamily R Member 1, CYP27B1: Cytochrome P450 Family 27 Subfamily B Member 1. The figure was created in BioRender.com and imported under the terms of premium subscription.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/05/178-1611554456-Figure2.jpg",
"caption": "Figure 2. Immunomodulatory roles of vitamin D. Vitamin D improves barrier function by promoting tighter junctions and maintaining barrier function in the epithelium. It also enhances expression of Antimicrobial peptides and inhibits dendritic cells and macrophages from causing excessive inflammation. Again, Vitamin D downregulates Th1 and Th17 responses as well as upregulates Th2 and Treg responses to abate the cytokine storm. AMP: antimicrobial peptide, Th: helper T cell, Treg: regulatory T cell, TNFα: tumor necrosis factor, IFN-γ: interferon γ, IL: interleukin. The figure was created in BioRender.com and imported under the terms of premium subscription.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/05/178-1611554456-Figure3.jpg",
"caption": "Figure 3. Vitamin D and Renin-Angiotensin System. The proposed therapeutic effect of Vitamin D as a negative regulator of the renin-angiotensin system that could reduce lung injury following SARS-CoV-2 infection. SARS-CoV-2 binds to ACE2 and causes a downregulation of ACE2. This increases Angiotensin II concentration and eventually leads to acute lung injury. Vitamin D, on the contrary, increases ACE2 concentration and thus lowers Angiotensin II concentration. ACE: angiotensin converting enzyme. The figure was created in BioRender.com and imported under the terms of premium subscription.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/01/05/178-1611554456-Figure4.jpg",
"caption": "Figure 4. Possible roles of vitamin D regarding pregnancy as determined from clinical outcomes and mouse models: in maternal health, maternal-fetal interface, in the fetus and later life of the child. hPL: human placental lactogen, hCG: human chorionic gonadotropin, SGA: small for gestational age, ALRI: acute lower respiratory infection. The figure was created in BioRender.com and imported under the terms of premium subscription.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/05/178-1611554456-Figure5.jpg",
"caption": "Figure 5. Possible roles of Vitamin D: protective role against depression and neuroprotection. By controlling neuronal calcium signaling, sustaining serotonin levels and enhancing dopamine production, vitamin D protects against major psychiatric diseases and depression, respectively. By reducing inflammatory molecules, increasing NGF and increasing oxidative function, vitamin D aids to maintain healthy neurones and prevent mitochondrial dysfunction respectively. NGF: nerve growth factor, TPH2: tryptophan hydroxylase 2, NT: neurotrophin. The figure was created in BioRender.com and imported under the terms of premium subscription.",
"featured": false
}
],
"authors": [
{
"id": 638,
"affiliation": [
{
"affiliation": "Biotechnology Program, Department of Mathematics and Natural Sciences, BRAC University, Dhaka, Bangladesh."
}
],
"first_name": "Nafisa",
"family_name": "Ahmed",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 152
},
{
"id": 639,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet,Bangladesh"
}
],
"first_name": "Yusha",
"family_name": "Araf",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 152
},
{
"id": 640,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh"
}
],
"first_name": "Md. Asad",
"family_name": "Ullah",
"email": "ullah1194@gmail.com",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Asad Ullah, Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh, Email: ullah1194@gmail.com",
"article": 152
}
]
},
{
"id": 151,
"slug": "178-1609794640-hematological-leukocytes-ratio-indices-predictors-of-acute-purulent-fecal-peritonitis-in-nonlinear-laboratory-rats",
"type": "original_article",
"manuscript_id": "178-1609794640",
"published": "2021-02-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/43/178-1609794640.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 120-132.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Lytvynenko RO, Makyeyeva VL. Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats. J Adv Biotechnol Exp Ther. 2021; 4(2): 120-132.",
"title": "Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats",
"abstract": "<p>The use of hematological leukocytes ratio indices (HLRI), which show the interrelations between different blood cells, for evaluation the organism state of laboratory rats is questionable. The aim is to investigate the informativity of HLRI in laboratory rats on the model of acute infectious process. The study performed on white nonlinear (7–8 months) male laboratory rats: control (n = 10) and experimental groups (n = 10), which simulated acute purulent fecal peritonitis. Analyzed the number of leukocytes, blood leukocyte formula, phagocytic activity of neutrophils, HLRI. Acute infectious process is accompanied by leukocytosis, neutrophilia with leukocyte blood formula shift to the left and inhibition of neutrophils absorption capacity. A set of changes of HLRI in experimental group animals reflects the predominance of cellular immunity over the humoral, prevalence of granulocytes and their immature forms, increased activity of the inflammatory process, predominance of the microphage system, intense non-specific immunity, disturbance of immune system functional state with a shift of balance towards monokines, formation of mainly delayed-type hypersensitivity reactions, decreased function of affector cells and predominance of effector part of immunological process, the infectious nature of intoxication. Analysis with receiver operating characteristic (ROC) curves displayed that 8 from 16 applied HLRI, such as blood leukocyte shift index, neutrophil to lymphocyte, segmented neutrophil to lymphocyte, banded neutrophil to lymphocyte, eosinophil to lymphocyte, monocyte to lymphocyte, neutrophil to monocyte and segmented neutrophil to monocyte count ratio indices have very high levels of sensitivity and specificity and can be used as diagnostic markers for acute purulent fecal peritonitis.</p>",
"DOI": "10.5455/jabet.2021.d113",
"views": 1262,
"downloads": 97,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/06/178-1609794640-Figure1.jpg",
"caption": "Figure 1. Morphology of blood cells of healthy laboratory rats and during fecal peritonitis: (A) small lymphocyte; (B) middle lymphocyte; (C) large lymphocyte with a synthetically active nucleus; (D) broad plasma lymphocyte with vacuolation of the cytoplasm (top), middle lymphocyte (bottom); (E) lymphocyte with synthetically active nucleus and basophilic cytoplasm, nucleus with indentation; (F) middle lymphocyte with cytoplasmic basophilia and azurophilic granules; (G) large lymphocyte with cytoplasmic basophilia, erythrocytes with spines (echinocytes); (H) atypical bi-nucleated lymphocyte; (I) atypical lymphocyte with cytoplasmic blebbing; (J) atypical lymphocyte, on the right rouleaux of erythrocytes; (K) atypical reactive lymphocyte; (L) monocyte with a bean-shaped nucleus; (M) monocyte with bean-shaped nucleus and vacuolation of cytoplasm; (N) monocyte with atypical (irregularly shaped) nucleus and vacuolation of cytoplasm (left), segmented neutrophil (right); (O) monocyte with an atypical nucleus (top), lymphocyte (bottom); (P) orthochromatic erythroblast; (Q, R) banded eosinophil; (S) banded eosinophil, echinocytes; (T) degranulation of the cytoplasm of banded eosinophil (×1000, Pappenheim stain).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/06/178-1609794640-Figure2.jpg",
"caption": "Figure 2. Morphology of blood neutrophils in healthy laboratory rats and in fecal peritonitis: (A, B, C) myelocyte; (D) young neutrophil; (E) myelocyte (top), segmented neutrophil (bottom); (F-L) banded neutrophil; (G, H) Döhle bodies in the cytoplasm can be differentiated near the cell membrane; (M) segmented neutrophil; (N) segmented neutrophil (right), lymphocyte (left), echinocyte, rouleaux of erythrocytes; (O) vacuolation of the cytoplasm of banded neutrophil; (P, Q) toxic granularity of neutrophil cytoplasm; (R) segmented neutrophil (left), vacuolation of the cytoplasm of banded neutrophil (right); (S) signs of karyorrhexis of the segmented neutrophil nucleus, rouleaux of erythrocytes, echinocytes are found; (T) signs of karyorrhexis of the segmented neutrophil nucleus, slight eosinophilia of the cytoplasm (×1000, Pappenheim stain).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/06/178-1609794640-Figure3.jpg",
"caption": "Figure 3. Receiver operating characteristic curves of HLRI for differentiating acute purulent fecal peritonitis from healthy state. The value of AUC ranges from 0 to 1. An AUC value of 0.50 indicates that HLRI did not perform better than random markers for differentiating acute purulent fecal peritonitis from healthy state, whereas a value of 1.0 indicates that HLRI can be used as predictors of acute purulent fecal peritonitis in nonlinear laboratory rats.",
"featured": false
}
],
"authors": [
{
"id": 633,
"affiliation": [
{
"affiliation": "Department of Physiology, Immunology and Biochemistry with the Course of Civil Defense and Medicine, Faculty of Biology, Zaporizhzhia National University, Zaporizhzhia, 69600, Ukraine"
}
],
"first_name": "Raisa Oleksandrivna",
"family_name": "Lytvynenko",
"email": "r_litvinenko@ukr.net",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Raisa Oleksandrivna Lytvynenko, Department of Physiology, Immunology and Biochemistry with the Course of Civil Defense and Medicine, Faculty of Biology, Zaporizhzhia National University, Zaporizhzhia, 69600, Ukraine, Email: r_litvinenko@ukr.net",
"article": 151
},
{
"id": 634,
"affiliation": [
{
"affiliation": "Department of Histology, Cytology and Embryology, Medical Faculty No. 2, Zaporizhzhia State Medical University, Zaporizhzhia, 69035, Ukraine"
}
],
"first_name": "Lyudmyla Valeriyivna",
"family_name": "Makyeyeva",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 151
}
]
},
{
"id": 149,
"slug": "178-1601578466-antagonistic-activity-of-exiguobacterium-indicum-lis01-isolated-from-sediment",
"type": "original_article",
"manuscript_id": "178-1601578466",
"published": "2021-02-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/07/178-1601578466.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(2): 114-119.",
"academic_editor": "Mahmudul Hasan Sikder, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Lisa AK, Alam MS, et al. Antagonistic activity of Exiguobacterium indicum LIS01 isolated from sediment. J Adv Biotechnol Exp Ther. 2021; 4(2): 114-119.",
"title": "Antagonistic activity of Exiguobacterium indicum LIS01 isolated from sediment",
"abstract": "<p>The bacterial resistance over antibiotics has gained significant concern worldwide for the last few decades. This study aimed to isolate and characterize antibiotic producing bacteria from different water sediment sources in Bangladesh. A total of 9 samples were collected from three different sediment sources from Gopalganj, Bangladesh. Bacterial isolates grown in nutrient agar with cycloheximide were tested for antagonistic activity following disc-diffusion assay. Based on preliminary careening, three isolates namely LIS01, LIS02 and LIS03 showed antibacterial activity against six clinical isolates: <em>E. coli, Salmonella </em>sp.,<em> Shigella </em>sp.,<em> Klebsiella pneumoniae, Streptococcus </em>sp., and <em>Staphylococcus </em>sp. The isolate LIS01 with the highest (P<0.05) zone of inhibition was characterized using 16S rRNA gene sequencing. According to phylogenetic analysis, the isolate was identified as <em>Exiguobacterium indicum</em> with more than 99.7% sequence similarity to previously characterized strains. The results of our study are very promising considering the antibiotic resistant bacteria and could be helpful in treating diseases associated with drug failure caused.</p>",
"DOI": "10.5455/jabet.2021.d112",
"views": 667,
"downloads": 110,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/06/178-1601578466-Figure1.jpg",
"caption": "Figure 1. Inhibitory activity of present study isolates (LIS01, LIS02 and LIS03) against six clinical bacteria. Bar with different superscripts indicates significantly different mean values at 0.05 level of significance.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/06/178-1601578466-Figure2.jpg",
"caption": "Figure 2. Phylogenetic tree showing the position and relationship of present study E. indicum LIS01 isolate to other strains characterized earlier. The tree was constructed using neighbour-joining method in MEGA 7. Streptomyces sp., and Pseudomonas sp., were considered as out group bacteria in the tree.",
"featured": false
}
],
"authors": [
{
"id": 629,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalgonj8100, Bangladesh"
}
],
"first_name": "Asura Khanam",
"family_name": "Lisa",
"email": "asura@bsmrstu.edu.bd",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Asura Khanam Lisa, Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj-8100, Bangladesh, Email: asura@bsmrstu.edu.bd",
"article": 149
},
{
"id": 630,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Most. Sumya",
"family_name": "Alam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 149
},
{
"id": 631,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalgonj8100, Bangladesh"
}
],
"first_name": "Abdullah-Al",
"family_name": "Jubayer",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 149
},
{
"id": 632,
"affiliation": [
{
"affiliation": "School of Public Health, Independent University, Bangladesh"
},
{
"affiliation": "Department of Pediatrics, Anwer Khan Modern Medical College Hospital, Dhaka, Bangladesh"
}
],
"first_name": "Fabia Hannan",
"family_name": "Mone",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 149
}
]
}
]
},
{
"id": 13,
"title": "Vol4 Issue1",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2021",
"slug": "2021-vol4-issue1",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 143,
"slug": "178-1607862738-challenges-in-medical-waste-management-amid-covid-19-pandemic-in-a-megacity-dhaka",
"type": "short_communication",
"manuscript_id": "178-1607862738",
"published": "2021-01-14",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/18/178-1607862738.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 106-113.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Faisal GM, Hoque MN, et al. Challenges in medical waste management amid COVID-19 pandemic in a megacity Dhaka. J Adv Biotechnol Exp Ther. 2021; 4(1): 106-113.",
"title": "Challenges in medical waste management amid COVID-19 pandemic in a megacity Dhaka",
"abstract": "<p>The COVID-19 pandemic has altered global waste generation dynamics, which is a challenging task for poor countries having inefficient waste management system. On an average, 6,180 tons of medical waste (MW) during this COVID-19 pandemic is generated per month in the Dhaka city. This voluminous amount of MW generated in the Dhaka city is remained poorly managed, and thus, posing a serious threat to public health and environment. To protect any risk of spread of SARS-CoV-2 through MW, a concerted and prompt effort from municipal authorities, hospital administration, and concerned non-government organization (NGOs) is needed to adopt new ways of state-of-the-art, safe and cost-effective MW management system for the Dhaka city. Furthermore, research should be directed to find out to find out other potential sources (e.g., inanimate objects or aquatic bodies) of SARS-CoV-2 infections to track it’s spatial and temporal dynamics, and also to get early warning in case of future outbreaks.</p>",
"DOI": "10.5455/jabet.2021.d111",
"views": 1527,
"downloads": 179,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/28/178-1607862738-Figure1.jpg",
"caption": "Figure 1. Spilling over of medical waste (MW) in the megacity of Dhaka, Bangladesh. (A) The biohazard bag containing COVID-19 waste (such as masks, gloves, head cover and personal protective equipment) along with other MW is thrown outside a dedicated COVID-19 hospital in Dhaka. (B) COVID-19 related waste mixed with general medical waste left in neighboring place of a hospital in Dhaka [37].",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/28/178-1607862738-Figure2.jpg",
"caption": "Figure 2. Present scenario of spilling over of medical waste (MW) in the megacity of Dhaka, Bangladesh. The biohazard bag containing COVID-19 waste (such as masks, gloves, head cover and personal protective equipment) along with other MW is thrown here and there, which sometimes mixed with household wastes, and left in open dumping places. The generated MW sometimes discharged to the river Buriganga through various open canals mixed with general waste, and thus creating a public health risk [37, 38].",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/02/28/178-1607862738-Figure3.jpg",
"caption": "Figure 3. Proposed strategies for medical waste (MW) management in the megacity of Dhaka. Images were collected from the open data source [36-38].",
"featured": false
}
],
"authors": [
{
"id": 596,
"affiliation": [
{
"affiliation": "Faculty of Veterinary Medicine and Animal Sciences, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Golam Mahbub",
"family_name": "Faisal",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 143
},
{
"id": 597,
"affiliation": [
{
"affiliation": "Faculty of Veterinary Medicine and Animal Sciences, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "M. Nazmul",
"family_name": "Hoque",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 143
},
{
"id": 598,
"affiliation": [
{
"affiliation": "Department of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "M. Shaminur",
"family_name": "Rahman",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 143
},
{
"id": 599,
"affiliation": [
{
"affiliation": "Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Tofazzal",
"family_name": "Islam",
"email": "tofazzalislam@yahoo.com",
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-7613-0261",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Tofazzal Islam, PhD; Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh, Email: tofazzalislam@yahoo.com",
"article": 143
}
]
},
{
"id": 142,
"slug": "178-1603105853-preliminary-analysis-of-phytochemicals-and-in-vitro-free-radical-scavenging-activity-of-dhanwantaram-kashayam",
"type": "original_article",
"manuscript_id": "178-1603105853",
"published": "2021-01-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/03/178-1603105853.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 95-105.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, South Korea",
"cite_info": "Renganathan S, Pillai RG.Preliminary analysis of phytochemicals and in vitro free radical scavenging activity of Dhanwantaram Kashayam. J Adv Biotechnol Exp Ther. 2021; 4(1): 95-105.",
"title": "Preliminary analysis of phytochemicals and in vitro free radical scavenging activity of Dhanwantaram Kashayam",
"abstract": "<p><em>Dhanwantaram Kashayam</em> (DK) is a polyherbal decoction used in Ayurveda for the postnatal care of mothers and for treating gynaecological diseases. It is also used as a growth stimulant in children as well as a regenerative medicine. Present study was to assess the various phytochemicals present in DK and to elucidate the role of DK as an effective antioxidant and free radical scavenger. Phytochemicals such as total phenolic content, total flavonoids and total tannins were assessed using standard biochemical methods. This study also investigated it’s <em>in vitro</em> antioxidant activity by ferric reducing antioxidant power (FRAP) assay and the free radical scavenging activity by assessing the scavenging activities on 2’2-Diphenyl-1-Picryl Hydrazine (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), phosphomolybdenum, hydrogen peroxide, nitric oxide and the hydroxyl radical. Phytochemical assay reported fairly high levels of tannins 0.22 ± 0.015mg/g, total flavonoids 1.23 ± 0.043 mg/g and total poly phenolic content 10.05 ± 0.94 mg/g. DK was found to have very good antioxidant activity and it scavenged the different free radicals in a dose dependent manner with low IC<sub>50</sub> values (DPPH: 2.08±0.051, ABTS:38.46±2.75, phosphomolybdenum 50.4±2.63, H<sub>2</sub>O<sub>2</sub>: 57.9±3.15, NO: 57.25±3.7, all expressed in µg/ml. Values were significant with p<0.05). Results of this study clearly revealed that the DK is rich in phytochemicals and is a good source of natural antioxidants and is an efficient scavenger of peroxide radicals. This supports the use of DK in Ayurveda as a regenerative medicine, but further studies are needed to correlate the <em>in vitro</em> observations with its pharmacological effects <em>in vivo</em>.</p>",
"DOI": "10.5455/jabet.2021.d110",
"views": 1419,
"downloads": 153,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/28/178-1603105853-Figure1.jpg",
"caption": "",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/28/178-1603105853-Figure2.jpg",
"caption": "",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/28/178-1603105853-Figure3.jpg",
"caption": "",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/28/178-1603105853-Figure4.jpg",
"caption": "",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/13/28/178-1603105853-Figure5.jpg",
"caption": "",
"featured": false
}
],
"authors": [
{
"id": 594,
"affiliation": [
{
"affiliation": "Department of Life Sciences, University of Calicut, Calicut University PO, 673635, India"
}
],
"first_name": "Smitha",
"family_name": "Renganathan",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 142
},
{
"id": 595,
"affiliation": [
{
"affiliation": "Department of Life Sciences, University of Calicut, Calicut University PO, 673635, India"
}
],
"first_name": "Radhakrishna Gopala",
"family_name": "Pillai",
"email": "pillai_radhakrishna@hotmail.com",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Radhakrishna G Pillai, PhD; Department of Life Sciences, University of Calicut, India, Email: pillai_radhakrishna@hotmail.com",
"article": 142
}
]
},
{
"id": 141,
"slug": "178-1603396401-assessing-drug-repurposing-option-for-emerging-viral-diseases-concerns-solutions-and-challenges-for-forthcoming-viral-battles",
"type": "review_article",
"manuscript_id": "178-1603396401",
"published": "2020-12-14",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/24/178-1603396401.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 74-94.",
"academic_editor": "Md. Abdul Hannan, PhD; Dongguk University, South Korea",
"cite_info": "Sarwar SB, Khondokar F, et al. Assessing drug repurposing option for emerging viral diseases: concerns, solutions, and challenges for forthcoming viral battles. J Adv Biotechnol Exp Ther. 2021; 4(1): 74-94.",
"title": "Assessing drug repurposing option for emerging viral diseases: concerns, solutions, and challenges for forthcoming viral battles",
"abstract": "<p>Since the beginning of time, microorganisms have been in existence. With time, new pathogens have emerged as a result of complex interplay of anthropogenic and natural factors like, human migration, shifts in weather pattern, genetic shuffling of the organisms themselves and more which have been discussed in detail. This review article focuses solely on emerging and re-emerging viruses: Chikungunya, Coronavirus, Dengue, Ebola, Hepatitis C, Herpes Simplex Virus (HSV), Human Immunodeficiency Virus (HIV), Human Papilloma Virus (HPV), Influenza and Zika; and the latest progresses made in finding effective antiviral drugs via drug repurposing as we know this approach outplays de novo production significantly with respect to time and money. In a time where new diseases are being reported once every year, drug repurposing will certainly come in handy in developing antiviral therapeutics promptly. Moreover, the article also discusses major challenges in drug repurposing from finding a patent, dealing with all relevant governing frameworks, to careful and safe handling of viruses, these are some challenges faced by drug repurposing, to name a few. Additionally, the study elaborates on the mechanisms of actions of these drugs as well as the targets whilst including recent and well-known incidences of deadly, viral outbreaks.</p>",
"DOI": "10.5455/jabet.2021.d109",
"views": 1987,
"downloads": 118,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/49/03/178-1603396401-Figure1.jpg",
"caption": "Figure 1. Factors of disease emergence and connected events.",
"featured": false
}
],
"authors": [
{
"id": 587,
"affiliation": [
{
"affiliation": "Biotechnology Program, Department of Mathematics and Natural Science, School of Data and Sciences, Brac University, Dhaka, Bangladesh"
}
],
"first_name": "Subyeta Binte",
"family_name": "Sarwar",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 141
},
{
"id": 588,
"affiliation": [
{
"affiliation": "Biotechnology Program, Department of Mathematics and Natural Science, School of Data and Sciences, Brac University, Dhaka, Bangladesh"
}
],
"first_name": "Faiza",
"family_name": "Khondokar",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 141
},
{
"id": 589,
"affiliation": [
{
"affiliation": "Biotechnology Program, Department of Mathematics and Natural Science, School of Data and Sciences, Brac University, Dhaka, Bangladesh"
}
],
"first_name": "Hiya",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 141
},
{
"id": 590,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh"
}
],
"first_name": "Md. Asad",
"family_name": "Ullah",
"email": "ullah1194@gmail.com",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Asad Ullah, Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh, E-mail: ullah1194@gmail.com",
"article": 141
},
{
"id": 591,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet,Bangladesh"
}
],
"first_name": "Yusha",
"family_name": "Araf",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 141
},
{
"id": 592,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh"
}
],
"first_name": "Bishajit",
"family_name": "Sarkar",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 141
},
{
"id": 593,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "MD. Hasanur",
"family_name": "Rahman",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 141
}
]
},
{
"id": 136,
"slug": "178-1603391777-antibacterial-potential-of-synthesized-silver-nanoparticles-from-leaf-extract-of-moringa-oleifera",
"type": "original_article",
"manuscript_id": "178-1603391777",
"published": "2020-11-29",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1603391777.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 67-73.",
"academic_editor": "Akhi Moni, PhD; ABEx Bio-Research Center, Dhaka 1230, Bangladesh",
"cite_info": "Islam A, Mandal C, et al. Antibacterial potential of synthesized silver nanoparticles from leaf extract of Moringa oleifera. J Adv Biotechnol Exp Ther. 2021; 4(1): 67-73.",
"title": "Antibacterial potential of synthesized silver nanoparticles from leaf extract of Moringa oleifera",
"abstract": "<p>Silver nanoparticles (Ag-NPs) are among the most widely used nanoparticles that show a broad spectrum of antibacterial activity. Green synthesis of Ag-NPs from plant extract is the most popular method <em>in vitro</em>. In this study, Ag-NPs were biologically synthesized from the leaf extract of <em>Moringa oleifera</em> and the antimicrobial activity was observed. Furthermore, a comparative assessment of antimicrobial activity of biologically synthesized Ag-NPs and crude plant extract was performed. Initially, 11 pathogenic bacterial strains were used to evaluate the antibacterial potentiality of crude leaf sample. However, the result showed that the crude leaf extract exerted maximum potential against <em>Proteus vulgaris</em>. Additionally, the application of biologically synthesized Ag-NPs was assessed against the same pathogenic strains and observed enhanced antibacterial activities with larger inhibited zones. Thus, our results suggest that the biological synthesis of Ag-NPs significantly enhanced the antibacterial activity of <em>M. oleifera</em> leaf extract against selected pathogenic bacterial strains. <em>M. oleifera</em> could be a potential source of Ag-NPs for successful use as an antibacterial agent in pharmaceutical and cosmetic industries.</p>",
"DOI": "10.5455/jabet.2021.d108",
"views": 1438,
"downloads": 165,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/40/02/178-1603391777-Figure1.jpg",
"caption": "Figure 1. Overview of the green synthesis procedure of Ag-NPs using sunlight as the primary energy source. The leaves were dried and powdered to dissolve in 50% of ethyl acetate for preparing crude extract. After seven days of incubation the mixture was filtered and evaporated in water bath. The concentrated extract was then incubated for 72 h at 45 0C. The extract was ready for further analysis and can be stored at 4 0C. The biological synthesis of Ag-NPs involved the mixture of silver nitrate solution with plant extract followed by the direct sunlight exposure for 30 min. Subsequent change in color to dark brown indicated the formation of nanoparticles in the reaction tubes. After purification the synthesized Ag-NPs were further confirmed using a UV-vis spectrometer at 300-700 nm. Then, both of the crude extract and Ag-NPs were subjected to evaluate their antibacterial activities against pathogenic bacterial strains.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/40/02/178-1603391777-Figure2.jpg",
"caption": "Figure 2. Synthesis of Ag-NPs and its confirmation. A-C represents confirmation of nanoparticle formation by visual observation. Silver nitrate solution was mixed with leaf extract and exposed to sunlight for 30 min. During the incubation period the color of the crude extracts were gradually changed to brown color. After 30 min of sunlight exposure the color reached to the dark brown. The change of color indicates the formation of nanoparticles in the reaction tubes. D showing the UV-vis absorption spectra of synthesized silver nanoparticles as a function of time. The control represents the reaction mixture of AgNO3 solution with distilled water.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/40/02/178-1603391777-Figure3.jpg",
"caption": "Figure 3. Fold enrichment of antibacterial activities of synthesized Ag-NPs when compared to crude extracts. These bar graphs showed the relative fold enhancement of Ag-NPs from crude leaf extract. Values are represented as the average fold change ± SEM bars, n = 3 replicates. Asterisks indicate statistically significant changes based on adjusted p values < 0.05.",
"featured": false
}
],
"authors": [
{
"id": 564,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh."
}
],
"first_name": "Anti",
"family_name": "Islam",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 136
},
{
"id": 565,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh."
}
],
"first_name": "Chanchal",
"family_name": "Mandal",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 136
},
{
"id": 566,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh."
}
],
"first_name": "Ahsan",
"family_name": "Habib",
"email": "ahsan_habib@ku.ac.bd",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ahsan Habib, PhD, Professor, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh, Email: ahsan_habib@ku.ac.bd",
"article": 136
}
]
},
{
"id": 135,
"slug": "178-1601659915-prevalence-of-single-nucleotide-polymorphism-308ga-in-the-tnf-a-promoter-region-correlates-coronary-heart-disease-among-type-2-diabetic-patients-from-the-northern-region-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1601659915",
"published": "2020-11-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/11/178-1601659915.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 60-66.",
"academic_editor": "Mohammad Nazrul Islam, PhD; Sher-e-Bangla Agricultural University, Bangladesh",
"cite_info": "Mahmud MRA, Hasan MM, et al. Prevalence of single-nucleotide polymorphism (-308G>A) in the TNF-α promoter region correlates coronary heart disease among type-2 diabetic patients from the northern region of Bangladesh. J Adv Biotechnol Exp Ther. 2021; 4(1): 60-66.",
"title": "Prevalence of single-nucleotide polymorphism (-308G>A) in the TNF-α promoter region correlates coronary heart disease among type-2 diabetic patients from the northern region of Bangladesh",
"abstract": "<p>Tumor necrosis factor-alpha (TNF-α) is a major cytokine for inflammatory response in human body. This is also well linked with obesity and causing different pathophysiological problems in type 2 diabetic mellitus (T2DM) patients because of its pro-inflammatory over expression. However, seemingly harmless nucleotide changes in the promoter region often cause oscillation in expression, results in complications like dyslipidemia and atherosclerosis that ultimately exploit to coronary heart disease (CHD). Therefore, this study was designed to evaluate association between TNF-α (-308G>A) polymorphism at promoter region and CHD in T2DM patients from the northern region (Rajshahi) of Bangladesh. The total number of participants was 96 T2DM patients. TNF-α polymorphism were detected using high resolution melting (HRM) curve analysis. A total of 32 participants were suffering from CHD and 8 polymorphism (3 homozygous and 5 heterozygous) were detected among them. From Fisher’s Exact test, we found significant (P < 0.05) relationship between TNF-α (-308G>A) polymorphism and CHD in T2DM patients. According to Kendall’s Tau correlation matrix (r = 0.218), there is a good correlation between target polymorphism and CHD. Therefore, the overall results suggest that TNF-α promoter (-308G>A) polymorphism influences CHD in T2DM patients.</p>",
"DOI": "10.5455/jabet.2021.d107",
"views": 679,
"downloads": 104,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/57/02/178-1601659915-Figure1.jpg",
"caption": "Figure 1. HRM analysis for the genotypes of TNF-α (-308G>A) polymorphism at promoter region. (A) Wild type normalized curve of samples from healthy (no history of CVD and T2DM) individuals, (B) difference curve of experimental samples of T2DM patients.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/57/02/178-1601659915-Figure2.jpg",
"caption": "Figure 2. Sequencing result showing conversion of G (black peak) to A (green peak) at -308 position of TNF-α promoter region.",
"featured": false
}
],
"authors": [
{
"id": 550,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Rezuan Al",
"family_name": "Mahmud",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 135
},
{
"id": 551,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Mahmudul",
"family_name": "Hasan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 135
},
{
"id": 552,
"affiliation": [
{
"affiliation": "Molecular Pathology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi 6205, Bangladesh"
}
],
"first_name": "Dipa",
"family_name": "Roy",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 135
},
{
"id": 553,
"affiliation": [
{
"affiliation": "Department of Microbiology, Ad-din Sakina Medical College, Jessore, Bangladesh"
},
{
"affiliation": "Molecular Pathology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi 6205, Bangladesh"
}
],
"first_name": "Md. Sk Shahid",
"family_name": "Ullah",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 135
},
{
"id": 560,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh"
}
],
"first_name": "Bristy",
"family_name": "Basak",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 135
},
{
"id": 561,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Abu",
"family_name": "Reza",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 135
},
{
"id": 562,
"affiliation": [
{
"affiliation": "Molecular Pathology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi 6205, Bangladesh"
}
],
"first_name": "Ariful",
"family_name": "Haque",
"email": "haque@ru.ac.bd",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ariful Haque, Associate Professor, Molecular Pathology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi 6205, Bangladesh, Email: haque@ru.ac.bd",
"article": 135
}
]
},
{
"id": 130,
"slug": "178-1597659391-effect-of-chemical-and-physical-mutagens-on-amylase-producing-potentiality-of-aspergillus-flavus-nsh9",
"type": "original_article",
"manuscript_id": "178-1597659391",
"published": "2020-10-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/00/178-1597659391.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 53-59.",
"academic_editor": "Md. Niamul Haque, PhD; Incheon National University, South Korea",
"cite_info": "Ruaida A, Karim KMR. Effect of chemical and physical mutagens on amylase producing potentiality of Aspergillus flavus NSH9. J Adv Biotechnol Exp Ther. 2021; 4(1): 53-59.",
"title": "Effect of chemical and physical mutagens on amylase producing potentiality of Aspergillus flavus NSH9",
"abstract": "<p><em>Aspergillus flavus</em> NSH9 is a potential source of α-amylase and glucoamylases. Between two of its glucoamylases, one can digest the raw starch granules, while both being pH and thermostable. The purpose of the study was to enhance the production of amylases (α-amylase and Glucoamylase) from <em>A. flavus</em> NSH9 by random mutagenesis using Ultraviolet (UV) irradiation and Ethidium bromide (EtBr) treatment. Glucoamylase and α-amylase activity were evaluated by 3, 5-dinitrosalicyclic acid (DNS) method and starch-iodine method, respectively. Mutated <em>A. flavus</em> NSH9 by EtBr treatment (10µg/ml) in PDA plate produced highest amount of both crude glucoamylases (GA) (1.47 ± 0.087 U/mL/min) and raw starch degrading glucoamylase (RSD-GA) (0.839 ± 0.036 U/mL/Hour). The highest value was 1.6 and 1.5 times higher compared to control for GA and RSD-GA, respectively. Here as, UV induced radiation produced about 1.3 and 1.4 times more GA and RSD-GA compared to control, respectively. The activity of α-amylase was about 1.7 times higher in the treatment group with 5µg/ml EtBr in sublethal condition than without the treatment group. In conclusion, both UV and EtBr treatment increased the amylases production from <em>A. flavus</em> NSH9. As the single mutation process of <em>A. flavus</em> NSH9 enhanced all three enzymes, the strains could be used for the commercial production of amylase.</p>",
"DOI": "10.5455/jabet.2021.d106",
"views": 771,
"downloads": 100,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/02/178-1597659391-Figure1.jpg",
"caption": "Figure 1. Glucose standard curve generated with DNS method at 540 nm. The concentration of glucose used in this study was ranging from 0.05 mg/mL to 0.25 mg/mL.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/02/178-1597659391-Figure2.jpg",
"caption": "Figure 2. Absorbance of the starch-iodine complex standard curve generated with soluble starch at 580nm. The amount of starch used in the standard curve was ranging from 50 to 400 microgram. Calculated, Y = 10.0428 Absorbance (starch-iodine complex) at 580 nm for 1 mg of starch.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/02/178-1597659391-Figure3.jpg",
"caption": "Figure 3. Crude glucoamylase activity in different treatment by chemical and physical mutagens. The highest activity was recorded from mutated A. flavus NSH9 by EtBr in PDA plate (10µg/mL). Error bars show standard deviation among three independent observations.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/02/178-1597659391-Figure4.jpg",
"caption": "Figure 4. Raw Starch Degrading glucoamylase (RSDG) activity in different treatment by chemical and physical mutagens. The highest activity was recorded from mutated NSH9 by EtBr in PDA plate (10µg/mL). Error bars show standard deviation among three independent observations.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/02/178-1597659391-Figure5.jpg",
"caption": "Figure 5. α-amylase activity in different treatment by chemical and physical mutagens. The highest activity was recorded from A. flavus NSH9 by EtBr at sublethal condition in the culture (5 µg/mL). Error bars show standard deviation among three independent observations.",
"featured": false
}
],
"authors": [
{
"id": 533,
"affiliation": [
{
"affiliation": "Institute of Nutrition and Food Science, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Ashika",
"family_name": "Ruaida",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 130
},
{
"id": 534,
"affiliation": [
{
"affiliation": "Institute of Nutrition and Food Science, University of Dhaka, Dhaka-1000, Bangladesh"
}
],
"first_name": "Kazi Muhammad Rezaul",
"family_name": "Karim",
"email": "rkarim98@gmail.com",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Kazi Muhammad Rezaul Karim, Associate Professor, Institute of\r\nNutrition and Food Science, University of Dhaka, Dhaka-1000,\r\nBangladesh, Email: rkarim98@gmail.com",
"article": 130
}
]
},
{
"id": 120,
"slug": "178-1597401409-comparative-molecular-analysis-of-contemporary-isolates-of-duck-plague-virus-from-haor-areas-of-bangladesh",
"type": "short_communication",
"manuscript_id": "178-1597401409",
"published": "2020-10-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/16/178-1597401409.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 44-52.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Khan MT, Pavel MTR, et al. Comparative molecular analysis of contemporary isolates of duck plague virus from haor areas of Bangladesh. J Adv Biotechnol Exp Ther. 2021; 4(1): 44-52.",
"title": "Comparative molecular analysis of contemporary isolates of duck plague virus from haor areas of Bangladesh",
"abstract": "<p>Duck plague (DP) is one of the most important viral diseases which affects the duck population across the globe including Bangladesh. The present work was conducted to detect DP virus (DPV) from <em>haor</em> areas using a molecular-based approach and compared with the contemporary isolate through molecular and phylogenetic analysis. For this purpose, 38 individual samples were collected from the Netrokona (n=20) district of the Mymensingh division and Kishoreganj (n=18) district of the Dhaka division. The identification of DVP was carried out by polymerase chain reaction (PCR) targeting DPV specific <em>DNA polymerase </em>genes followed by sequencing. PCR positive viral samples were used to propagate into 11-13 days old embryonated duck eggs through chorio-allantoic membrane (CAM) route for virus isolation. DPV were then propagated into duck embryo fibroblast (DEF) monolayer cell culture and confirmed by PCR. Among the 38 samples, 27 isolates were confirmed as DPV with the PCR amplicon size of 446 bp. Pathogenicity tests through the inoculation into day-old ducklings confirmed pathogenic strain. The PCR products of the isolated DPV specific DNA polymerase gene were sequenced commercially and submitted to GenBank (GenBank Accession No. KX768734.1). The sequence showed resemblance to isolates previously reported in India (GenBank Accession No. KX511893.1, KJ451479.1, KM012009.1), and China (GenBank Accession No. EF643559.1). Sequencing data also revealed nucleotide differences between Anatid herpes 1_BAU_DMH (previous report from our laboratory) and the present isolates. Further characterization, such as nucleotide and amino acid sequencing, would help to understand the strains along with its epidemiology.</p>",
"DOI": "10.5455/jabet.2021.d105",
"views": 1041,
"downloads": 146,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/03/178-1597401409-Figure1.jpg",
"caption": "Figure 1. Maps of sampling locations. Dash circle indicating sadar upazila of Netrokona district and solid circle indicating Tarail upazila of Kishoreganj district. (Source: http://www.mapsofbangladesh.com/)",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/03/178-1597401409-Figure2.jpg",
"caption": "Figure 2. Results of propagation of present isolates of DPV in embryonated duck eggs. (a) normal embryo remained intact and CAM (arrow); (b) hemorrhagic thickened CAM and (c) petechial hemorrhages over the embryo’s body with no feather in DPV inoculated embryo; (d) Slide hemagglutination test mixing allantoic fluid from DPV infected embryo and 2% cRBC (chicken RBC) showed no hemagglutination.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/03/178-1597401409-Figure3.jpg",
"caption": "Figure 3: Results of propagation of the present isolates of duck plague virus into duck embryo fibroblast cell culture. (a) Control: Growth pattern of duck embryo fibroblast cells (400X magnification), kept as control, showing confluent growth of star like fibroblast cells. (b) Infected: Observation CPE of duck embryo fibroblast cells by duck plague virus showing round, necrotic, degeneration of cells, clumping of cell and giant cell formation of fibroblast cells under green light (400X magnification)(arrow: clumping of cell; dash circle: giant cell; solid circle: round cell).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/03/178-1597401409-Figure4.jpg",
"caption": "Figure 4. Results of pathogenicity test for the duck plague virus isolates. (a) Dead duckling after 6 days of post inoculation of DP virus; postmortem examination of ducklings showing (b) unclotted free blood in the body cavities, (c) petechial hemorrhage in the pale liver, (d) hemorrhagic annular band in intestine.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/03/178-1597401409-Figure5.jpg",
"caption": "Figure 5. PCR amplification products of duck plague virus with DNA polymerase gene specific primer. Lane M: 100 bp ladder; Lane 1: positive control; Lane 2-6: positive samples of duck plague virus; Lane 7: negative control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/03/178-1597401409-Figure6.jpg",
"caption": "Figure 6. Comparative analysis of the nucleic acid sequenced data of the present isolates and Ahamed et al 2015 [10]. The nucleotide variation was found in the 374-379 position. The ‘solid box’ indicated the difference region and the ‘dash box’ indicated the nucleotide sequence which was not reported in the previous study from our laboratory (i.e., Ahamed et al., 2015, reported 378 bp out of 446 bp amplicon).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/03/178-1597401409-Figure7.jpg",
"caption": "Figure 7. Phylogenetic tree showing relationship of DPV isolates. The tree was constructed through the neighbor joining method and nucleotide distances were measures through Jukes-Cantor method using CLC Sequence Viewer 8.0 software. Numbers on the nodes indicate bootstrap percentage values (calculated using 100 replicates). Red color represents this study isolates.",
"featured": false
}
],
"authors": [
{
"id": 480,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Most. Tahmina",
"family_name": "Khan",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 120
},
{
"id": 481,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Tahmid Rahman",
"family_name": "Pavel",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 120
},
{
"id": 482,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Afsana",
"family_name": "Keya",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 120
},
{
"id": 483,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Ahosanul Haque",
"family_name": "Shahid",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 120
},
{
"id": 484,
"affiliation": [
{
"affiliation": "Veterinary Surgeon, Department of Livestock Services, Peoples Republic of Bangladesh"
}
],
"first_name": "Tangila",
"family_name": "Ferdausi",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 120
},
{
"id": 485,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mahbubul Pratik",
"family_name": "Siddique",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 120
},
{
"id": 486,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Muhammad Tofazzal",
"family_name": "Hossain",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 120
},
{
"id": 487,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "KHM Nazmul Hussain",
"family_name": "Nazir",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 120
},
{
"id": 488,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Tanvir",
"family_name": "Rahman",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 120
},
{
"id": 489,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Bahanur",
"family_name": "Rahman",
"email": "bahanurr@bau.edu.bd",
"author_order": 10,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Bahanur Rahman, PhD; Professor, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, Email: bahanurr@bau.edu.bd",
"article": 120
}
]
},
{
"id": 114,
"slug": "178-1596045892-phytochemical-constituency-profiling-and-antimicrobial-activity-screening-of-seaweed-extracts-collected-from-the-bay-of-bengal-sea-coasts",
"type": "original_article",
"manuscript_id": "178-1596045892",
"published": "2020-10-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/26/178-1596045892.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 25-34.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Imran MAS, Bhuiyan FR, et al. Phytochemical constituency profiling and antimicrobial activity screening of seaweed extracts collected from the Bay of Bengal sea coasts. J Adv Biotechnol Exp Ther. 2021; 4(1): 25-34.",
"title": "Phytochemical constituency profiling and antimicrobial activity screening of seaweed extracts collected from the Bay of Bengal sea coasts",
"abstract": "<p>Seaweeds are able to produce a great variety of secondary metabolites that are characterized by a broad spectrum of biological activities. Two seaweeds species, namely <em>Hypnea musciformis </em>and <em>Enteromorpha intestinalis </em>were studied to evaluate the phytochemical constituency and antimicrobial activities. First of all, crude extracts of both sea weeds were prepared by two different extraction methods (soaking and water bath) using different solvents. Phytochemicals profiling results revealed the presence of bioactive compounds (flavonoids, alkaloids, tannin, saponin and phenols) in both seaweed extracts. Quantification results for ethanolic extracts of <em>H. musciformis and E. intestinalis </em>estimated 51 mg and 43 mg tannins in per gram of dried samples and flavonoids contents were found 67 mg and 57 mg/g (Quercetin equivalents) QE/g, respectively. Total phenolic contents were determined in terms of gallic acid equivalent (GAE). <em>H. musciformis </em>exhibited higher amount of phenolics (59 ± 0.0002 mg GAE/g) than <em>E. intestinalis </em>extracts (41 ± 0.0002 mg GAE/g).In antimicrobial activity test, ethanol extracts<em> of H. musciformis </em>and <em>E. intestinalis </em>were found 10 mm of inhibition diameter against all of the bacterial strains. Besides, methanol extracts of <em>E. intestinalis </em>were more susceptible to <em>Staphylococcus aureus and </em><em>Pseudomonas</em> which was close to the inhibition diameter (>15 mm) of the mainstream antibiotic, gentamicin. Moreover, <em>Klebsiella sp. </em>was found more susceptible to ethanol and methanol extracts of <em>H. musciformis </em>as it showed inhibition zone greater than 15 mm. Both Seaweed extracts possessed higher amount of phytochemicals and showed promising antimicrobial activities when compared with the standards.</p>",
"DOI": "10.5455/jabet.2021.d103",
"views": 1207,
"downloads": 133,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/03/178-1596045892-Figure1.jpg",
"caption": "Figure 1. Sample collection site; North Nuniar Chor, Cox’s bazaar, Banglades (21°35′0″N 92°01′0″E)",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/03/178-1596045892-Figure2.jpg",
"caption": "Figure 2. The pictorial view of Enteromorpha intestinalis and Hypnea musciformis at North Nuniar Chor, Cox’s bazaar, Bangladesh.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/03/178-1596045892-Figure3.jpg",
"caption": "Figure 3. Growth inhibition zone of different extracts of H. musciformis and E. intestinalis. (A) E. intestinalis ethanol extract against E. coli (soaking method). (B) E. intestinalis and H. musciformis methanol extract (soaking method) against S. aureus. (C) H. musciformis ethanol extract against Klebsiella sp. (Water bath method). (D) E. intestinalis and H. musciformis acetone extracts against S. aureus (soaking method). (E) Gentamicin against Klebsiella sp. (F) Gentamicin against Staphylococcus aureus.",
"featured": false
}
],
"authors": [
{
"id": 458,
"affiliation": [
{
"affiliation": "Department of Pharmaceuticals and Industrial Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Md. Abdus Shukur",
"family_name": "Imran",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 114
},
{
"id": 462,
"affiliation": [
{
"affiliation": "Department of Botany, University of Chittagong, Chittagong-4331, Bangladesh"
}
],
"first_name": "Farhana Rumzum",
"family_name": "Bhuiyan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 114
},
{
"id": 463,
"affiliation": [
{
"affiliation": "Department of Plant and Environmental Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Sheikh Rashel",
"family_name": "Ahmed",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 114
},
{
"id": 464,
"affiliation": [
{
"affiliation": "Department of Molecular Biology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Parsha",
"family_name": "Shanzana",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 114
},
{
"id": 465,
"affiliation": [
{
"affiliation": "Department of Pharmaceuticals and Industrial Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Mahmuda Akter",
"family_name": "Moli",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 114
},
{
"id": 466,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh"
}
],
"first_name": "Shakhawat Hossain",
"family_name": "Foysal",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 114
},
{
"id": 467,
"affiliation": [
{
"affiliation": "Department of Pharmaceuticals and Industrial Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Suma Bala",
"family_name": "Dabi",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 114
},
{
"id": 468,
"affiliation": [
{
"affiliation": "Department of Pharmaceuticals and Industrial Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Mahmudul",
"family_name": "Hasan",
"email": "mhasan.pib@sau.ac.bd",
"author_order": 8,
"ORCID": "https://orcid.org/0000-0003-4761-2111",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mahmudul Hasan, Professor; Department of Pharmaceuticals\r\nand Industrial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh\r\nE-mail: mhasan.pib@sau.ac.bd",
"article": 114
}
]
},
{
"id": 117,
"slug": "178-1598378684-screening-of-antagonistic-potential-bacteria-from-rhizosphere-soil-against-phytopathogenic-fungi-related-to-selected-vegetable-crops",
"type": "original_article",
"manuscript_id": "178-1598378684",
"published": "2020-10-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/14/178-1598378684.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 35-43.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Seoul, South Korea",
"cite_info": "Alam MK, Rahman MM, et al. Screening of antagonistic potential bacteria from rhizosphere soil against phytopathogenic fungi related to selected vegetable crops. J Adv Biotechnol Exp Ther. 2021; 4(1): 35-43.",
"title": "Screening of antagonistic potential bacteria from rhizosphere soil against phytopathogenic fungi related to selected vegetable crops",
"abstract": "<p>Fungal phytopathogens cause serious losses of crop production worldwide, which causes serious losses of crop production. Bacteria play a role as the biocontrol agents for plant disease control. For this reason, the present study was conducted to determine the antagonistic potential of rhizosphere soil bacteria against selected phytopathogenic fungi. The screenings of potential antagonist isolated bacteria were applied by the dual culture technique with fungi <em>Fusarium oxysporum </em>and <em>Colletotrichum melongenae</em>. Molecular characterization was performed through 16S rDNA sequencing analysis. Sixteen (16) out of fifty (50) isolated bacteria showed different degrees of antagonism (25-67%) against both fungi <em>F. oxysporum </em>and <em>C. melongenae.</em> Among them, four (4) isolated bacteria (isolates A4, C1, C2, and E2) exhibited strong antagonism (more than 50% mycelial growth inhibition) against both fungi. The 16S rDNA sequences of the isolated bacteria A4, C1, C2 and E2 were 99-100% similar to <em>Providencia</em> sp. TT14, <em>Bacillus subtilis</em> 168, <em>Bacillus subtilis</em> RKP-2, and <em>Bacillus amyloliquefaciens</em> IBSDG-11, respectively. Based on the capability for the control of mycelial growth against both fungi, <em>B. subtilis</em> IUBTC2 was selected for optimization of growth characteristics and identification the bioactive metabolites which can enhance plant growth and disease control capacity of plants against the phytopathogenic fungi.</p>",
"DOI": "10.5455/jabet.2021.d104",
"views": 1544,
"downloads": 278,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/03/178-1598378684-Figure1.jpg",
"caption": "Figure 1. Antagonistic activity of isolated bacteria against F. oxysporum and C. melongenae in dual culture in PDA after 4 days of culture at 27ºC. (a-d and f-i). Fungal growth was inhibited towards the direction of isolated bacteria and (e and j) control plates inoculated with only the fungal isolate.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/03/178-1598378684-Figure2.jpg",
"caption": "Figure 2. Antagonistic activities of potential isolated bacteria against F. oxysporum and C. melongenae. Selected bacteria, A4- Providencia spp. TT14, C1- B. subtilis 168, C2- B. subtilis RKP-2, and E2-B. amyloliquefaciens IBSDG-11.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/03/178-1598378684-Figure3.jpg",
"caption": "Figure 3. Growth patterns of the selected isolated bacteria. Selected bacteria, A4- Providencia spp. TT14, C1- B. subtilis 168, C2- B. subtilis RKP-2, and E2-B. amyloliquefaciens IBSDG-11in different culture media (a) Tryptone soya agar (TAS), (b) lactose broth agar (LBA), (c) yeast extract agar (YEA), (d) potato dextrose agar (PDA) and (e) King’s medium B agar (KBA) after 66 h of incubation at 37ºC",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/03/178-1598378684-Figure4.jpg",
"caption": "Figure 4. Neighbor-joining phylogenetic trees showing relationships of isolated bacteria a) A4- Providencia sp. TT14, b) C1- B. subtilis 168, c) C2- B. subtilis RKP-2, and d) E2-B. amyloliquefaciens IBSDG-11 with closely related strains based on their 16S rDNA sequences.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/03/178-1598378684-Figure5.jpg",
"caption": "Figure 5. Schematic diagram of summary.",
"featured": false
}
],
"authors": [
{
"id": 472,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh."
}
],
"first_name": "Md. Khasrul Alam",
"family_name": "Alam",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 117
},
{
"id": 473,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh."
}
],
"first_name": "M Mizanur",
"family_name": "Rahman",
"email": "rahmanmm@btge.iu.ac.bd",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-4225-9451",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Prof. Dr. Md. Mizanur Rahman, Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh. E-mail: rahmanmm@btge.iu.ac.bd",
"article": 117
},
{
"id": 474,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh."
}
],
"first_name": "Abu Hena Mostafa",
"family_name": "Jamal",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 117
},
{
"id": 475,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh."
}
],
"first_name": "Md. Rezuanul",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 117
}
]
},
{
"id": 109,
"slug": "178-1597893403-nutritional-status-and-associated-factors-in-children-aged-15-17-years-in-a-suburb-in-vietnam",
"type": "original_article",
"manuscript_id": "178-1597893403",
"published": "2020-09-24",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/24/178-1597893403.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 17-24.",
"academic_editor": "Md Jamal Uddin, PhD; Ewha Womans University, Korea",
"cite_info": "Chu DT, Dao DTA, et al. Nutritional status and associated factors in children aged 15-17 years in a suburb in Vietnam. J Adv Biotechnol Exp Ther. 2021; 4(1): 17-24.",
"title": "Nutritional status and associated factors in children aged 15-17 years in a suburb in Vietnam",
"abstract": "<p>A double nutritional burden (DBM) has increased rapidly in Vietnam. Finding a solution to reduce the rates of overweight & obesity and thinness was necessary, especially at the age of 15-17 years. This study aimed to investigate nutritional status and elucidate the associated factors in children aged 15-17 years in the suburban of Vietnam. A cross-sectional study was carried out from November to December 2019 in Vietnam. Total of 424 students aged 15-17 years (153 boys and 271 girls) from Hong Thai Highschool using a randomized sampling method. The nutritional status of studied subjects was determined based on the World Health Organization (WHO) criteria of sex and age specific BMI cut-off points for children between 5 and 19 years old (2007). Rates of overweight and obesity was 8.96%, thinness was 8.02%, there was a statistically significant difference between boys’ and girls’ group (p=0.02). Correlation between BMI and WC, HC showed that WC or HC could be another indicator to determine the nutritional status of children. Having milk or a light meal before going to bed was also found to decrease the risk of being overweight by 66.4% (OR = 0.336, 95% CI: 0.116 – 0.972). Eating as much as possible increases the risk of overweight and obesity 2.2 times (p=0.017). Moreover, fast food consumption and physical activity time were no significant difference between the obesity & overweight and non-overweight groups. Especially, compared with the walking group, the group with adults taken had the risk of overweight and obesity 14 times higher, the group traveling by motorbike had the risk of overweight and obesity 5.056 times higher (with p=0.018 and p=0.04, respectively). The results suggest that there was the DBM for children in suburban, nutrition status was not similar among boys and girls. Public health programs should therefore be developed or improved in order to promote good eating habits and physical activity, especially how to move to school among children in Vietnam.</p>",
"DOI": "10.5455/jabet.2021.d102",
"views": 725,
"downloads": 140,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/03/178-1597893403-Figure1.jpg",
"caption": "Figure 1. Prevalence of severe thinness, thinness, normal, overweight, and obesity defined by BMI of (A) Total studied subjects, (B) Boy group, and (C) Girl group.",
"featured": false
}
],
"authors": [
{
"id": 428,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy Road, Cau Giay District, Ha Noi, Vietnam."
}
],
"first_name": "Dinh-Toi",
"family_name": "Chu",
"email": "chudinhtoi.hnue@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dinh-Toi Chu, PhD; Faculty of Biology, Hanoi National University of Education, Hanoi, Vietnam. Email: chudinhtoi.hnue@gmail.com",
"article": 109
},
{
"id": 429,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy Road, Cau Giay District, Ha Noi, Vietnam."
}
],
"first_name": "Duong Thi Anh",
"family_name": "Dao",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 109
},
{
"id": 430,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy Road, Cau Giay District, Ha Noi, Vietnam."
}
],
"first_name": "Le Thi",
"family_name": "Duyen",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 109
},
{
"id": 431,
"affiliation": [
{
"affiliation": "Faculty of Clinical Pharmacy, Hanoi University of Pharmacy, Hanoi, Vietnam"
}
],
"first_name": "Bui Nhat",
"family_name": "Le",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 109
},
{
"id": 432,
"affiliation": [
{
"affiliation": "Institute of Preventive Medicine and Public Health, Hanoi Medical University, Hanoi, Vietnam"
}
],
"first_name": "Vu Thi",
"family_name": "Hue",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 109
},
{
"id": 434,
"affiliation": [
{
"affiliation": "Department of Nutrition, Le Huu Trac National Institute of Burns, Vietnam Military Medical University, Hanoi, Vietnam"
}
],
"first_name": "Vu Tri",
"family_name": "Quang",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 109
},
{
"id": 433,
"affiliation": [
{
"affiliation": "Department of Anatomy, Vietnam Military Medical University, Hanoi, Vietnam"
}
],
"first_name": "Dang Tien",
"family_name": "Truong",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 109
},
{
"id": 435,
"affiliation": [
{
"affiliation": "Department Obstetrics and Gynecology, Hanoi Medical University, Hanoi, Vietnam"
}
],
"first_name": "Thang Nguyen",
"family_name": "Manh",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 109
}
]
},
{
"id": 107,
"slug": "178-1597540568-qualitative-phytochemicals-and-pharmacological-properties-analysis-of-methanol-extract-of-tabernaemontana-pauciflora-leaves",
"type": "original_article",
"manuscript_id": "178-1597540568",
"published": "2020-09-15",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/39/178-1597540568.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 09-16.",
"academic_editor": "Akhi Moni, PhD; ABEx Bio-Research Center, Bangladesh",
"cite_info": "Hossen A, Arman M, et al. Qualitative phytochemicals and pharmacological properties analysis of methanol extract of Tabernaemontana pauciflora leaves. J Adv Biotechnol Exp Ther. 2021; 4(1): 09-16.",
"title": "Qualitative phytochemicals and pharmacological properties analysis of methanol extract of Tabernaemontana pauciflora leaves",
"abstract": "<p>The methanol extract of the leaves of <em>Tabernaemontana pauciflor</em>a (METP) was screened to investigate the qualitative phytochemical, <em>in vivo</em> anti-depressant, anxiolytic and <em>in vitro</em> thrombolytic activities. The <em>in vivo</em> antidepressant activity of METP was examined by force swimming test (FST) and tail suspension test (TST), whereas anxiolytic activity was examined by using hole-board test (HBT) and elevated plus maze test (EPM) on mice model. Additionally, qualitative phytochemical assay was performed to identify some secondary metabolites, namely alkaloids, carbohydrates, flavonoids. The <em>in vitro</em> thrombolytic model was used to evaluate the clot lysis effect of METP. In the antidepressant assay, METP (200 and 400 mg/kg) exhibited significant anti-depression activity <em>(P < 0.001</em>) on FST (45.64% and 58.31%) and TST (38.67% and 63.62%) whereas the notable anxiolytic effect was observed in EPM (67.67% and 56.06%) and HBT (35.53% and 50%) in dose dependent manner which compared with control. In <em>in vitro</em> thrombolytic assessment of METP exposed 22.66% inhibition of clot. The current results proposed that METP might be effective in neuropharmacological and thrombolytic response due to the presence of alkaloids, tannins and flavonoids, which required further studies to investigate the mechanism.</p>",
"DOI": "10.5455/jabet.2021.d101",
"views": 1180,
"downloads": 110,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/03/178-1597540568-Figure1.jpg",
"caption": "Figure 1. (A) Anxiolytic action of methanol extract of T. pauciflora (leaves), control and diazepam on percentage of time spent in open arm in elevated plus maze test (EPM). (B) Anxiolytic effect of methanol extract of T. pauciflora (leaves), diazepam and control on number of head-dipping in hole-board test (HBT). Values are represented in Mean ± SEM (n = 5). In both test METP dose 400 mg/kg exhibited P values (**P< 0.001) is statistically significant in comparison to control followed by Dunnett test of one-way ANOVA. METP = Methanol extract of Tabernaemontana pauciflora (leaves).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/03/178-1597540568-Figure2.jpg",
"caption": "Figure 2. (A) Antidepressant activity of methanol extract of T. pauciflora (leaves) and fluoxetine on force swimming test (FST). (B) Antidepressant effect of methanol extract of T. pauciflora (leaves) and fluoxetine on tail suspension test (TST). Values are represented in Mean ± SEM (n = 5). **P< 0.001 statistically significant in comparison to control followed by Dunnett test of one-way ANOVA. METP = Methanol extract of Tabernaemontana pauciflora (leaves).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/03/178-1597540568-Figure3.jpg",
"caption": "Figure 3. The clot lysis activity of methanol extract of T. pauciflora (leaves), streptokinase and normal saline (Control). Values are represented in Mean ± SEM (n = 5). METP dose displayed P value (**P< 0.001) is statistically significant in comparison to control followed by Dunnett test of one-way ANOVA. METP = Methanol extract of Tabernaemontana pauciflora (leaves).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/03/178-1597540568-Figure4.jpg",
"caption": "Figure 4. Graphical representation of methanol extract of T. pauciflora (leaves) on qualitative phytochemical, antidepressant, anxiolytic and thrombolytic activity.",
"featured": false
}
],
"authors": [
{
"id": 415,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Anowar",
"family_name": "Hossen",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 107
},
{
"id": 416,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammad",
"family_name": "Arman",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 107
},
{
"id": 417,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Syed Al Jawad",
"family_name": "Sayem",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 107
},
{
"id": 418,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammad Rifat Alam",
"family_name": "Maruf",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 107
},
{
"id": 419,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Md. Ahsan",
"family_name": "Ullah",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 107
},
{
"id": 420,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammad Nazmul",
"family_name": "Islam",
"email": "sayeadiiuc@gmail.com",
"author_order": 6,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Nazmul Islam, Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh, Email: sayeadiiuc@gmail.com",
"article": 107
},
{
"id": 421,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Naureen",
"family_name": "Banu",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 107
},
{
"id": 422,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Najmul",
"family_name": "Alam",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 107
},
{
"id": 423,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Shahenur Alam",
"family_name": "Sakib",
"email": "sakibhasaniiuc@gmail.com",
"author_order": 9,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Shahenur Alam Sakib, Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh, E-mail: sakibhasaniiuc@gmail.com",
"article": 107
}
]
},
{
"id": 105,
"slug": "178-1595827549-adaptations-of-muscular-biology-in-response-to-potential-glucocorticoid-treatment-in-broiler-chicken",
"type": "original_article",
"manuscript_id": "178-1595827549",
"published": "2020-09-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/51/178-1595827549.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2021; 4(1): 01-08.",
"academic_editor": "Md Nabiul Islam, PhD; Yamaguchi University, Japan",
"cite_info": "Akter F, Sultana N, et al. Adaptations of muscular biology in response to potential glucocorticoid treatment in broiler chicken. J Adv Biotechnol Exp Ther. 2021; 4(1): 01-08.",
"title": "Adaptations of muscular biology in response to potential glucocorticoid treatment in broiler chicken",
"abstract": "<p>Poultry meat production has been dramatically increased in the last few decades due to increased population rate. Glucocorticoids decrease the growth of poultry and increase fat accumulation in liver and meat. In the coming days, it is important to consider the quality of meat to fulfill the increasing demand of proteins. The morphological and biometric properties of meat are associated with the quality of meat. The present research aimed to study the adaptations of muscular biology in response to potential glucocorticoid treatment in broiler chicken. This experiment was conducted into three groups of broilers (i.e. control group: homemade ration, group A: commercial broiler ration, and group B: a high dose of glucocorticoid at 7 mg/kg) started from day 7 to 28. Meat and blood samples were collected at day 7, 14, 21, and 28. For gross morphology, color and weight of meat were measured. Histomorphology of meat was studied under light microscope by Hematoxylin & Eosin stain. The length and width of meat fibers were measured using calibrated stage micrometer. The blood cholesterol dynamics was measured by spectrophotometer. The color of breast meat was more yellowish and lighter than thigh meat. The weight of meat was negatively affected by glucocorticoid. Glucocorticoid treatment negatively influenced the number of myofibers in breast meat, while positively influenced the thigh meat. Excess dietary glucocorticoid increased the biometry of breast meat and decreased that of thigh meat in broiler. Glucocorticoid non-significantly increased the serum cholesterol level. These findings advance our knowledge about the action of glucocorticoid in the muscular system and provide basis for novel therapies to prevent glucocorticoid-induced muscular atrophy.</p>",
"DOI": "10.5455/jabet.2021.d100",
"views": 1235,
"downloads": 178,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/03/178-1595827549-Figure1.jpg",
"caption": "Figure 1. a. Representative photos of breast and thigh meat color of broilers in group B at day 14 of the experiment. b. Histological representation of breast and thigh meat fibers (40X). Bm- breast meat, tm- thigh meat.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/03/178-1595827549-Figure2.jpg",
"caption": "Figure 2. Effects of glucocorticoid on the weight of breast and thigh meat (g) in relation to the days of the experiment in broiler. Data were expressed as mean ± standard error and differences among the groups of birds were compared using one-way ANOVA with post-hoc Duncan’s multiple range test. Column with differing superscripts are significantly different from each other (P < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/03/178-1595827549-Figure3.jpg",
"caption": "Figure 3. Effects of glucocorticoid on the number of breast and thigh meat fibers in relation to days of the experiment in broiler chicken. Data were expressed as mean ± standard error and differences among the groups of birds were compared using one-way ANOVA with post-hoc Duncan’s multiple range test. Column with differing superscripts are significantly different from each other (P < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/03/178-1595827549-Figure4.jpg",
"caption": "Figure 4. Effects of glucocorticoid on the length of breast and thigh meat fibers (µm) in relation to the days of the experiment in broiler chicken. Data were expressed as mean ± standard error and differences among the groups of birds were compared using one-way ANOVA with post-hoc Duncan’s multiple range test. Column with differing superscripts are significantly different from each other (P < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/03/178-1595827549-Figure5.jpg",
"caption": "Figure 5. Effects of glucocorticoid in the width of breast and thigh meat fibers (µm) in relation to the days of the experiment in broiler chicken. Data were expressed as mean ± standard error and differences among the groups of birds were compared using one-way ANOVA with post-hoc Duncan’s multiple range test. Column with differing superscripts are significantly different from each other (P < 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/03/178-1595827549-Figure6.jpg",
"caption": "Figure 6. Dynamics of cholesterol in serum in relation to age of broiler chicken treated with glucocorticoid. Data were expressed as mean ± standard error and differences among the groups of birds were compared using one-way ANOVA with post-hoc Duncan’s multiple range test.",
"featured": false
}
],
"authors": [
{
"id": 406,
"affiliation": [
{
"affiliation": "Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Ferdoushi",
"family_name": "Akter",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 105
},
{
"id": 407,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Nasrin",
"family_name": "Sultana",
"email": "nasrin.sultana@bau.edu.bd",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nasrin Sultana, PhD; Department. of Anatomy and Histology, Bangladesh\r\nAgricultural University, Mymensingh-2202, Bangladesh, E-mail: nasrin.sultana@bau.edu.bd",
"article": 105
},
{
"id": 408,
"affiliation": [
{
"affiliation": "Department of Life Science and Veterinary Medicine, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, Bangladesh"
}
],
"first_name": "Marzia",
"family_name": "Afrose",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 105
},
{
"id": 409,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Alamgir",
"family_name": "Kabir",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 105
},
{
"id": 410,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 105
},
{
"id": 411,
"affiliation": [
{
"affiliation": "Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mahmudul Hasan",
"family_name": "Sikder",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 105
}
]
}
]
}
],
"title": "2021",
"slug": "2021",
"order": 4
},
{
"id": 1,
"issues": [
{
"id": 1,
"title": "Special Issue",
"cover": null,
"special_issue_title": "COVID-19: conventional therapies, fates, and mechanisms",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": "2020-12-15",
"volume": "2020",
"slug": "special-issue4-2020",
"special_issue": true,
"topic_editors": [
{
"name": "Dinh-Toi Chu, PhD",
"image": null,
"affiliations": "Vietnam",
"website": "https://www.researchgate.net/profile/Md-Jamal-Uddin"
},
{
"name": "Md. Masudur Rahman, PhD",
"image": null,
"affiliations": "Sylhet Agricultural University, Bangladesh",
"website": "https://www.researchgate.net/profile/Md-Rahman-316"
}
],
"info": "<p style=\"text-align: justify;\">Dear Colleagues,</p>\r\n<p style=\"text-align: justify;\">An outbreak of the novel coronavirus, COVID-19, rapidly spread around the world in 2020. Currently, there are many unanswered questions about this novel coronavirus, e.g., the conventional therapies, herbal therapies, physio-therapies, exercise, fatality rate, fates after recovering etc. Biotechnologists, biomedical scientists, biologists, bioinformaticians can help us to understand such issues and make substantial contributions to explain the therapies and their mechanisms. This Special issue will collect timely papers on modeling studies concerning the biological, immunological, molecular, and virological aspects of COVID-19. This Special Issue aims to bring together biotechnologists, biomedical scientists, molecular biologists, bioinformaticians, biophysicists, and medical doctors to improve our understanding of the disease by using various approaches.</p>\r\n<p style=\"text-align: justify;\">Dinh-Toi Chu, PhD, Editor</p>\r\n<p style=\"text-align: justify;\">Md. Masudur Rahman, PhD, Editor</p>",
"issue_articles": [
{
"id": 103,
"slug": "178-1600458731-prediction-of-sars-cov-2-main-protease-inhibitors-in-medicinal-plant-derived-compounds-by-molecular-docking-approach",
"type": "original_article",
"manuscript_id": "178-1600458731",
"published": "2020-11-14",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/27/178-1600458731.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 79-96.",
"academic_editor": "Md. Abdul Hannan, PhD; Dongguk University, South Korea",
"cite_info": "Farabi S, Saha NR, et al. Prediction of SARS-CoV-2 main protease inhibitors in medicinal plant-derived compounds by molecular docking approach. J Adv Biotechnol Exp Ther. 2020; 3(4): 79-96.",
"title": "Prediction of SARS-CoV-2 main protease inhibitors in medicinal plant-derived compounds by molecular docking approach",
"abstract": "<p>Coronaviruses are endemic in humans and infections typically mild, such as the common cold. Still, the cross-species transmission has produced some unusually virulent strains which now causing viral pneumonia, in severe cases, even acute respiratory distress syndrome and death. SARS-CoV-2 is the most threatening issue which leads the world to an uncertainty alongside thousands of regular death scenes. An effective vaccine to cure this virus is not yet available, so it requires concerted efforts at various scales. The viral Main Protease controls coronavirus replication and is a proven drug discovery target for SARS-CoV-2. Comprehensive computational study e.g., molecular docking and ADMET (absorption, distribution, metabolism and excretion) profiling were employed to predict the efficacy of medicinal plant-based bioactive compounds against SARS-CoV-2 MPP. Paritaprevir and lopinavir-previously approved viral main protease inhibitors were used as standards for comparison. MPP was docked with 90 phytochemical compounds, and the screening revealed that four compounds (azadirachtin, -12.5 kcal/mol; rutin, -9 kcal/mol; theaflavin, -9 kcal/mol; astragalin, -8.8 kcal/mol) showed the highest binding affinity than the controls paritaprevir and lopinavir (-8.7 and -7.9 kcal/mol, respectively). Comparative structural analysis of protein-inhibitor complexes revealed that the compounds have intense interaction with the vital catalytic residue His-41 and Cys-145. Furthermore, the pharmaco-kinetics and drug-likeness properties of the antiviral phytochemicals suggested that the compounds do not have any considerable detrimental effects and can be considered potential drug candidates against SARS-CoV-2. These compounds can be further explored for in vitro experimental validation against SARS-CoV-2.</p>",
"DOI": "10.5455/jabet.2020.d159",
"views": 1450,
"downloads": 182,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/07/178-1600458731-Figure1.jpg",
"caption": "Figure 1. Phylogeny study of SARS-CoV-2, SARS-CoV and MERS-CoV by Neighbor Joining Method of MEGA X.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/07/178-1600458731-Figure2.jpg",
"caption": "Figure 2. (a) Ribbon representation of the superimposed SARS-CoV-2 MPP (magenta) (PDB ID 6LU7) bound to inhibitor N3 (green sticks), SARS-CoV MPP (cyan) bound to an aza-peptide epoxide inhibitor (red sticks) (PDB: 2A5I) and MERS-CoV MPP (orange) bound to GC813 (blue sticks) (PDB: (5WKK). (b) Active site residues of the main proteases.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/07/178-1600458731-Figure3.jpg",
"caption": "Figure 3. Molecular interaction between Paritaprevir and Main Protease Protein of SARS-CoV-2.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/07/178-1600458731-Figure4.jpg",
"caption": "Figure 4. Molecular insights of Main Protease Protein interactions with (a) Azadirachtin (-12.5 kcal/mol) (b) Rutin (-9.0 kcal/mol) (c) Theaflavin (-9.0 kcal/mol).",
"featured": false
}
],
"authors": [
{
"id": 394,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sayma",
"family_name": "Farabi",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 103
},
{
"id": 395,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Nihar Ranjan",
"family_name": "Saha",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 103
},
{
"id": 396,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md.",
"family_name": "Hasanuzzaman",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 103
},
{
"id": 397,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Noushin Anika",
"family_name": "Khan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 103
},
{
"id": 398,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Muhammad Shahidul",
"family_name": "Haque",
"email": "haquems@bau.edu.bd",
"author_order": 5,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Muhammad Shahidul Haque, Department of Biotechnology,\r\nBangladesh Agricultural University, Mymensingh-2202, Bangladesh\r\nEmail: haquems@bau.edu.bd",
"article": 103
}
]
},
{
"id": 102,
"slug": "178-1597656445-pathophysiological-mechanisms-of-disease-severity-in-covid-19-an-update",
"type": "review_article",
"manuscript_id": "178-1597656445",
"published": "2020-09-15",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/03/178-1597656445.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 68-78.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Bangladesh",
"cite_info": "Siam MHB, Abedin SMM, et al. Pathophysiological mechanisms of disease severity in COVID-19: An update. J Adv Biotechnol Exp Ther. 2020; 3(4): 68-78.",
"title": "Pathophysiological mechanisms of disease severity in COVID-19: An update",
"abstract": "<p>The pandemic of COVID-19 has created a confounding global health crisis with more than 25 million cases and 870,000 deaths worldwide. The novel coronavirus, SARS-CoV-2, exploits the human ACE2 receptor and potential CD147 to invade a plethora of organ systems including cardiovascular, renal, endocrine, nervous, and gastrointestinal. The pathophysiological mechanisms of COVID-19 infection were found to be associated with the direct viral invasion, dysregulated renin-angiotensin-aldosterone system (RAAS), hypoxia, hyperinflammation, cytokine storm, endotheliopathy, and thrombosis. Emerging evidence suggests that the kinin-kallikrein system, iron dysregulation, and complement component C5a anaphylatoxin have roles in disease severity. In critical patients, the effects manifest as acute respiratory distress syndrome (ARDS), lymphopenia, acute kidney injury (AKI), disseminated intravascular coagulation (DIC), hypovolemic shock leading to multiorgan dysfunction syndrome (MODS). In this review, we provided an update on the pathophysiology of COVID-19 with an emphasis on the clinical outcomes in severe patients that will help facilitate a deeper understanding of the disease.</p>",
"DOI": "10.5455/jabet.2020.d158",
"views": 2980,
"downloads": 208,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/24/07/178-1597656445-Figure1.jpg",
"caption": "Figure 1. A schematic diagram of COVID-19 pathophysiology. The SARS-CoV-2 binds to the ACE2 receptor and invades multiple organ systems. The dysregulation of the renin–angiotensin–aldosterone system (RAAS) and the Kinin-Kallikrein system contributes to increase permeability and vasodilation. The release of proinflammatory cytokines by white blood cells contributes to widespread inflammation, plasma leakage and fall in blood pressure. The development of ARDS, systemic infection, disseminated intravascular coagulation (DIC) and tissue ischemia lead to multiple organ dysfunction syndrome (MODS).",
"featured": false
}
],
"authors": [
{
"id": 389,
"affiliation": [
{
"affiliation": "Department of Emerging and Neglected Diseases, Biomedical Research Foundation, Dhaka, Bangladesh"
},
{
"affiliation": "Department of Microbiology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Md. Hasanul Banna",
"family_name": "Siam",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 102
},
{
"id": 390,
"affiliation": [
{
"affiliation": "Department of Microbiology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Shah MD Minhajul",
"family_name": "Abedin",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 102
},
{
"id": 391,
"affiliation": [
{
"affiliation": "Department of Emerging and Neglected Diseases, Biomedical Research Foundation, Dhaka, Bangladesh"
},
{
"affiliation": "Dhaka Medical College and Hospital, Dhaka, Bangladesh"
}
],
"first_name": "Ahsan",
"family_name": "Ahmed",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 102
},
{
"id": 392,
"affiliation": [
{
"affiliation": "Department of Emerging and Neglected Diseases, Biomedical Research Foundation, Dhaka, Bangladesh"
},
{
"affiliation": "Dhaka Medical College and Hospital, Dhaka, Bangladesh"
}
],
"first_name": "Nahida Hannan",
"family_name": "Nishat",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 102
},
{
"id": 393,
"affiliation": [
{
"affiliation": "Department of Emerging and Neglected Diseases, Biomedical Research Foundation, Dhaka, Bangladesh"
},
{
"affiliation": "School of Environment and Life Science, Independent University Bangladesh (IUB), Dhaka, Bangladesh"
}
],
"first_name": "Mohammad Sorowar",
"family_name": "Hossain",
"email": "sorowar.hossain@brfbd.org",
"author_order": 5,
"ORCID": "https://orcid.org/0000-0001-7143-2909",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Sorowar Hossain, PhD; Department of Emerging and Neglected Diseases, Biomedical Research Foundation, Dhaka, Bangladesh,\r\nEmail: sorowar.hossain@brfbd.org",
"article": 102
}
]
},
{
"id": 99,
"slug": "178-1593105436-identification-of-potential-sars-cov-2-main-protease-inhibitors-from-ficus-carica-latex-an-in-silico-approach",
"type": "original_article",
"manuscript_id": "178-1593105436",
"published": "2020-09-09",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/13/178-1593105436.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 57-67.",
"academic_editor": "Md. Masudur Rahman, PhD; Sylhet Agricultural University, Sylhet 3100, Bangladesh",
"cite_info": "Ali MC, Nur AJ, et al. Identification of potential SARS-CoV-2 main protease inhibitors from Ficus Carica Latex: An in-silico approach. J Adv Biotechnol Exp Ther. 2020; 3(4): 57-67.",
"title": "Identification of potential SARS-CoV-2 main protease inhibitors from Ficus Carica Latex: An in-silico approach",
"abstract": "<p>SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is the aetiological agent behind the current pandemic of coronavirus disease 2019 (COVID-19). SARS-CoV-2 main protease plays a dynamic role in mediating viral replication and transcription, which is one of the most probable drug targets against SARS-CoV-2. <em>Ficus carica </em>latex encompasses notable bioactive molecules with various biological properties, including antiviral activities. In this study, latex compounds of <em>Ficus carica</em> were screened to find out active phytochemicals against SARS-CoV-2 main protease through molecular docking, molecular dynamics simulation, and ADMET (absorption, distribution, metabolism, excretion, and toxicity) profiling. A total of 21 compounds were screened, and the compounds, lupeol, α-amyrin, and luteolin, showed the highest binding affinity and intense interaction with the vital catalytic residue His 41 and Cys 145. The molecular dynamics simulation revealed that the amyrin is the most stable compound with higher binding free energy, suggesting that this compound can compete with the native ligands of the main protease. The ADMET analysis indicated that these phytochemicals have considerable physicochemical, pharmacokinetics, and drug-likeness properties and do not possess any considerable detrimental effects and can be considered as potential drug candidates against SARS-CoV-2. However, further in-vitro, in-vivo, and clinical trials are required to observe the exact efficiency of these compounds.</p>",
"DOI": "10.5455/jabet.2020.d157",
"views": 1256,
"downloads": 172,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/36/07/178-1593105436-Figure1.jpg",
"caption": "Figure 1. The 2D structure of main protease. Here, the schematic diagram of domain I is represented by yellow color, domain II is represented by blue-green color, domain III is represented by dark red color, and the catalytic Cys-His dyad is represented in CYK form by red color.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/36/07/178-1593105436-Figure2.jpg",
"caption": "Figure 2. Docking scores of all compounds. Most of the compounds have moderate binding affinity.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/36/07/178-1593105436-Figure3.jpg",
"caption": "Figure 3. Molecular interactions of selected compounds with SARS-Cov-2 main protease (Mpro). (a) Molecular interactions of all latex compounds, the interaction of (b) positive control α-ketoamide with Mpro, (c) lupeol-Mpro, (d) α-amyrin-Mpro, and (e) luteolin- Mpro. All selected compounds interact with the vital substrate management catalytic dyad Cys 145-His 41.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/36/07/178-1593105436-Figure4.jpg",
"caption": "Figure 4. Molecular dynamics simulation. Analysis of (a) RMSD (Root Mean Square Deviation); (b) RMSF (Root Mean Square Fluctuations); (c) Rg (Radius of Gyration); (d) SASA (Solvent Accessible Surface Area); and (e) Binding free energy.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/36/07/178-1593105436-Figure5.jpg",
"caption": "Figure 5. Schematic diagram of summary.",
"featured": false
}
],
"authors": [
{
"id": 378,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Md. Chayan",
"family_name": "Ali",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 99
},
{
"id": 379,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Anjumana Jannati",
"family_name": "Nur",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 99
},
{
"id": 380,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Mst. Shanzeda",
"family_name": "Khatun",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 99
},
{
"id": 381,
"affiliation": [
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea."
}
],
"first_name": "Raju",
"family_name": "Dash",
"email": "rajudash.bgctub@gamil.com",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Raju Dash, Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea. Email: rajudash.bgctub@gamil.com",
"article": 99
},
{
"id": 382,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Md Mafizur",
"family_name": "Rahman",
"email": "shilon83@gmail.com",
"author_order": 5,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Mafizur Rahman, Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh. Email: shilon83@gmail.com",
"article": 99
},
{
"id": 383,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Mohammad Minnatul",
"family_name": "Karim",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 99
}
]
},
{
"id": 91,
"slug": "178-1594574470-prediction-of-b-cell-and-t-cell-epitopes-in-the-spike-glycoprotein-of-sars-cov-2-in-bangladesh-an-in-silico-approach",
"type": "original_article",
"manuscript_id": "178-1594574470",
"published": "2020-09-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/07/178-1594574470.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 49-56.",
"academic_editor": "Md Abdul Hannan, PhD; Dongguk University, South Korea",
"cite_info": "Islam MA, Shihab MMR, et al. Prediction of B-cell and T-cell epitopes in the spike glycoprotein of SARS-CoV-2 in Bangladesh: An in-silico approach. J Adv Biotechnol Exp Ther. 2020; 3(4): 49-56.",
"title": "Prediction of B-cell and T-cell epitopes in the spike glycoprotein of SARS-CoV-2 in Bangladesh: An in-silico approach",
"abstract": "<p>To combat highly infectious Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), scientists and researchers are toiling hard globally to develop effective drugs and vaccines. By exploring the structural proteins of SARS-CoV-2 can be a feasible way to find an effective vaccine. In this study by using in-silico tools, we recommended B-cell and T-cell epitopes of spike protein from a Bangladeshi isolate which can be considered for incorporation into a vaccine against the SARS-CoV-2. Homology modelling, energy minimization process, and finally Ramachandran model was used for the prediction of a more stable conformation of the spike protein. The most important peptides were screened through the VaxiJen server followed by the IEDB server and CTLPred Score predicted and analysed the desired epitopes. In the final analysis, the peptide EVRQIAPGQTGKIADY (starting from 91) showed the highest antigenicity score (1.3837) as a B-cell epitope although GSTPCNGVEGFNCYFP, starting at 161, showed highest score (0.91) in an initial analysis. On the contrary, as a T-cell epitope, 71 KLNDLCFTNV- 80 was found with the highest antigenicity score (2.6927) which was also found as an epitope in further analysis. A combination of B-cell and T-cell epitopes may evoke a humoral and cell-mediated immune response which will possibly lead to an effective vaccine. Further, the various computational analyses will provide valuable information that will pave the way for modelling a novel vaccine against SARS-CoV-2.</p>",
"DOI": "10.5455/jabet.2020.d156",
"views": 761,
"downloads": 143,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/03/07/178-1594574470-Figure1.jpg",
"caption": "Figure 1. Homology modeling of spike protein generated by SWISS-MODEL.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/03/07/178-1594574470-Figure2.jpg",
"caption": "Figure 2. 3-D model of the spike protein after energy optimization generated by using FoldX.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/03/07/178-1594574470-Figure3.jpg",
"caption": "Figure 3. Ramachandran plot using SWISS-MODEL.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/03/07/178-1594574470-Figure4.jpg",
"caption": "Figure 4. Predicted linear B-cell epitopes and their locations to the surface membrane (yellow) by Ellipro.",
"featured": false
}
],
"authors": [
{
"id": 341,
"affiliation": [
{
"affiliation": "Department of Microbiology, Faculty of Life and Earth Science, Jagannath University, Dhaka 1100, Bangladesh"
}
],
"first_name": "Mehedi",
"family_name": "Hasan",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 91
},
{
"id": 342,
"affiliation": [
{
"affiliation": "Department of Microbiology, Faculty of Life and Earth Science, Jagannath University, Dhaka 1100, Bangladesh"
}
],
"first_name": "Md. Maksudur Rahman",
"family_name": "Shihab",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 91
},
{
"id": 343,
"affiliation": [
{
"affiliation": "Department of Microbiology, Faculty of Life and Earth Science, Jagannath University, Dhaka 1100, Bangladesh"
}
],
"first_name": "Mohammad Ariful",
"family_name": "Islam",
"email": "ariful@mib.jnu.ac.bd",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Ariful Islam, PhD; Department of Microbiology, Faculty of Life and Earth Science, Jagannath University, Dhaka 1100, Bangladesh, Email: ariful@mib.jnu.ac.bd",
"article": 91
}
]
},
{
"id": 83,
"slug": "178-1594961238-japans-public-health-and-culture-and-the-ongoing-fight-against-covid-19",
"type": "review_article",
"manuscript_id": "178-1594961238",
"published": "2020-08-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1594961238.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 42-48.",
"academic_editor": "Dinh-Toi Chu, PhD; \r\nHanoi National University of Education, Vietnam.",
"cite_info": "Mahbub MH, Khan M, et al. Japan’s public health and culture, and the ongoing fight against COVID-19. J Adv Biotechnol Exp Ther. 2020; 3(4): 42-48.",
"title": "Japan’s public health and culture, and the ongoing fight against COVID-19",
"abstract": "<p>The rising number of new cases or new waves of COVID-19 infections threaten to overwhelm or collapse the health care system in many countries. The pandemic is likely to make the human sufferings worse. However, unlike many other developed countries, the situation in Japan with respect to COVID-19 remains relatively very good, despite the existence of a number of unfavorable factors that could make the country more vulnerable to COVID-19. Therefore, it seems important to understand and clarify the factors that have helped hold down the number of COVID-19 cases and deaths in Japan. The purpose of this narrative review was to provide some insights into the public health system and cultural factors that might have significantly contributed to the success of Japan in the fight against COVID-19. In light of the findings and discussion of this review, we suggest that Japan has achieved a remarkable success against COVID-19, by virtue of its strong and effective public health system, and also high standards of ingrained hygiene practices that are deeply influenced by local culture.</p>",
"DOI": "10.5455/jabet.2020.d155",
"views": 1757,
"downloads": 155,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/08/178-1594961238-Figure1.jpg",
"caption": "Figure 1. Number of deaths from COVID-19 in selected countries (per million people; as of 3 August 2020; according to data website Worldometer).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/08/178-1594961238-Figure2.jpg",
"caption": "Figure 2. Number of tests for COVID-19 in selected countries (per million people; as of 3 August 2020; according to data website Worldometer).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/08/178-1594961238-Figure3.jpg",
"caption": "Figure 3. Key components of the national campaign against COVID-19 – Avoid the “Three Cs”! – launched by Ministry of Health, Labour and Welfare Health, Japan [16].",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/08/178-1594961238-Figure4.jpg",
"caption": "Figure 4. Schematic diagram showing the main factors underlying the relatively low number of COVID-19 cases and associated deaths in Japan.",
"featured": false
}
],
"authors": [
{
"id": 311,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "MH",
"family_name": "Mahbub",
"email": "hossain@yamaguchi-u.ac.jp",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "MH Mahbub, Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Japan. Email: hossain@yamaguchi-u.ac.jp",
"article": 83
},
{
"id": 312,
"affiliation": [
{
"affiliation": "Digital Medical Systems Limited, Dhaka, 1205, Bangladesh."
}
],
"first_name": "Mohammadullah",
"family_name": "Khan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 83
},
{
"id": 313,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505,\r\nJapan"
}
],
"first_name": "Natsu",
"family_name": "Yamaguchi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 83
},
{
"id": 314,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Ryosuke",
"family_name": "Hase",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 83
},
{
"id": 315,
"affiliation": [
{
"affiliation": "Digital Medical Systems Limited, Dhaka, 1205, Bangladesh."
}
],
"first_name": "Noriaki",
"family_name": "Harada",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 83
},
{
"id": 316,
"affiliation": [
{
"affiliation": "Department of Public Health and Preventive Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Tsuyoshi",
"family_name": "Tanabe",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 83
}
]
},
{
"id": 82,
"slug": "178-1594050559-health-service-facilities-are-positively-linked-with-outcome-of-covid-19-patients-in-majority-of-the-countries-the-global-situation",
"type": "review_article",
"manuscript_id": "178-1594050559",
"published": "2020-08-09",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/21/178-1594050559.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 36-41.",
"academic_editor": "Dinh-Toi Chu, PhD\r\nHanoi National University of Education, Vietnam.",
"cite_info": "Islam MT, Talukder AK, et al. Health service facilities are positively linked with outcome of COVID-19 patients in majority of the countries: The global situation. J Adv Biotechnol Exp Ther. 2020; 3(4): 36-41.",
"title": "Health service facilities are positively linked with outcome of COVID-19 patients in majority of the countries: The global situation",
"abstract": "<p>Since emergence, coronavirus disease-19 (COVID-19) has extensively spread to >210 countries. Till date, no specific drug or vaccine has been developed against this deadly disease. Thus, all affected countries have been struggling to manage their COVID-19 patients. Here, we aimed to report impact of health service facilities on outcome of COVID-19 patients. As of June 28, 2020, the highest number of COVID-19 patients was reported in USA, which sum up to 2,617,847 with 4.9% death. Likewise, Spain, Italy, UK and France were greatly affected by COVID-19 with 10 to 18% death. Notably, Germany has been affected by COVID-19 in similar to UK and France with a total of 194,771 confirmed cases; however, recovery rate is very high (91.3%) with only 4.6% death. Alternatively, India and Bangladesh reported positive cases of 548,817 and 137,787, respectively. The comparison of health service facilities among different countries shows that Germany and also Russia have the highest number of doctors, hospital beds, ICU and ventilators in proportion to their people, which might contribute to restrict death rate only 1.4 to 4.6% with excellent recovery. USA has better health system with compared to that in China, India and Bangladesh; however, the recovery rate is 41.3 %, because the country is dealing with large number of patients. The limited health service facilities in Bangladesh might result in relatively lower recovery rate (40.4%) of COVID-19 patients. Thus, health service facilities of the nations are likely to be associated with successful management of COVID-19 patients.</p>",
"DOI": "10.5455/jabet.2020.d154",
"views": 551,
"downloads": 149,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/08/178-1594050559-Figure1.jpg",
"caption": "Figure 1. Health service management facilities in different countries of the world. The data show that Germany has very improved health care facilities for their people among the developed countries. On the other side, the developing countries such as Bangladesh and India have relatively poor health management services.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/08/178-1594050559-Figure2.jpg",
"caption": "Figure 2. Association of health service facilities with outcome of COVID-19 patients. In majority of cases, improved health service facilities are likely to enhance recovery and reduce death rate of COVID-19 patients. However, other factors (?) might also be involved on outcome of COVID-19 patients that may include age, food habit and immune status of the patient.",
"featured": false
}
],
"authors": [
{
"id": 307,
"affiliation": [
{
"affiliation": "Department of Pathobiology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh"
}
],
"first_name": "Md. Taimur",
"family_name": "Islam",
"email": "taimurpbl@bsmrau.edu.bd",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Taimur Islam, PhD;\r\nDepartment of Pathobiology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh\r\nE-mail: taimurpbl@bsmrau.edu.bd",
"article": 82
},
{
"id": 308,
"affiliation": [
{
"affiliation": "Department of Gynecology, Obstetrics and Reproductive Health, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh"
}
],
"first_name": "Anup Kumar",
"family_name": "Talukder",
"email": "anupbau@bsmrau.edu.bd",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Anup Kumar Talukder, PhD;\r\nDepartment of Gynecology, Obstetrics and Reproductive Health, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh, \r\nE-mail: anupbau@bsmrau.edu.bd",
"article": 82
},
{
"id": 309,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh"
}
],
"first_name": "Muhammad",
"family_name": "Badruzzaman",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 82
},
{
"id": 310,
"affiliation": [
{
"affiliation": "Department of Pathology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md. Abu Hadi Noor Ali",
"family_name": "Khan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 82
}
]
},
{
"id": 13,
"slug": "178-1594965742-current-knowledge-on-mechanisms-involved-in-sars-cov-2-infection-and-kidney-diseases",
"type": "review_article",
"manuscript_id": "178-1594965742",
"published": "2020-07-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/43/178-1594965742.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 30-35",
"academic_editor": "Dinh-Toi Chu, PhD \r\nHanoi National University of Education, Vietnam.",
"cite_info": "Rahman MH, Zahan MS, Hasib TA, etal. Current knowledge on mechanisms involved in SARS-CoV-2 infection and kidney diseases. J Adv Biotechnol Exp Ther. 2020 Dec; 3(4): 30-35.",
"title": "Current knowledge on mechanisms involved in SARS-CoV-2 infection and kidney diseases",
"abstract": "<p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is cause of a global pandemic which is demolishing global health and economy. SARS-CoV-2 infected patients are hospitalized with pneumonia where almost 20-30% of patients are led to kidney failure. The entry of SARS-CoV-2 into the systemic circulation leads to acute kidney injury (AKI) which may develop chronic kidney disease (CKD). In addition, patients who are diagnosed with AKI or CKD are at major risk of SARS-CoV-2 infection. Although a significant number of compounds have been proposed and the existing drugs have also been tested for repurposing, no specific therapy has been approved yet. SARS-CoV-2 invades human cells binding to the receptor of angiotensin-converting enzyme 2 (ACE2) via the receptor-binding domain. Cells that express ACE2 are susceptible to SARS-CoV-2 infection and the proportion of ACE2-positive cells in kidney proximal tubule is approximately 4%, indicating that SARS-CoV-2 might damage the kidney tubules leading to fatal kidney injury. Therefore, a better understanding of the potential mechanisms involved in SARS-CoV-2 infection-mediated kidney disease may unveil a novel therapeutic strategy against kidney diseases during COVID-19.</p>",
"DOI": "10.5455/jabet.2020.d153",
"views": 1847,
"downloads": 202,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/05/02/image-003.jpg",
"caption": "Figure 1. Possible mechanisms involved in the SARS-CoV-2 in kidney diseases. After binding spike protein of SARS-COV-2 with ACE2 receptor of the proximal tubule, it directly enters in the cells. Consequently, the release/activation of AKI inducing factors such as inflammatory cytokines, activation of C5b-9 complex, and induction of hypoxia (ROS, HIF-1) which may lead to mitochondrial injury, cell deaths, and loss of brush border. Along with these, the system inflammatory responses may induce tubular epithelial injury and fibrosis that ultimately causes CKD.",
"featured": true
}
],
"authors": [
{
"id": 4,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "MD. Hasanur",
"family_name": "Rahman",
"email": "hasanurrahman.bge@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-9238-3149",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 13
},
{
"id": 5,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Md. Sarwar",
"family_name": "Zahan",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-5768-4317",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 13
},
{
"id": 6,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Tasnin Al",
"family_name": "Hasib",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 13
},
{
"id": 7,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Kazi Ahsan",
"family_name": "Ahmed",
"email": null,
"author_order": 4,
"ORCID": "https://orcid.org/0000-0002-4831-7758",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 13
},
{
"id": 8,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Mushira",
"family_name": "Khanam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 13
},
{
"id": 10,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Akhi",
"family_name": "Moni",
"email": null,
"author_order": 6,
"ORCID": "http://orcid.org/0000-0002-5812-7755",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 13
},
{
"id": 9,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science & Technology, Bangladesh"
}
],
"first_name": "MD. Sadman",
"family_name": "Omit",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 13
},
{
"id": 11,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": "hasan800920@gmail.com",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0003-2911-3255",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Jamal Uddin, PhD \r\nGraduate School of Pharmaceutical\r\nSciences, College of Pharmacy, Ewha\r\nWomans University, Seoul 03760,\r\nRepublic of Korea,",
"article": 13
}
]
},
{
"id": 73,
"slug": "178-1592312166-covid-19-transmission-diagnosis-policy-intervention-and-potential-broader-perspective-on-the-rapidly-evolving-situation-in-bangladesh",
"type": "review_article",
"manuscript_id": "178-1592312166",
"published": "2020-07-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/23/178-1592312166.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 18-29.",
"academic_editor": "Dr. Md. Masudur Rahman, Sylhet Agricultural University, Bangladesh.",
"cite_info": "Mina FB, Billah M, et al. COVID-19: transmission, diagnosis, policy intervention, and potential broader perspective on the rapidly evolving situation in Bangladesh. J Adv Biotechnol Exp Ther. 2020; 3(4): 18-29.",
"title": "COVID-19: transmission, diagnosis, policy intervention, and potential broader perspective on the rapidly evolving situation in Bangladesh",
"abstract": "<p>Following the first outbreak of COVID-19 in China, various continents became serious and aware to combat against it, though degraded dramatically preventing it, due to its unique transmission strategy. On March 8, 2020, Bangladesh confirmed its first cases of COVID-19 with three people being infected and the first death was reported on March 18, 2020, until June 29, 2020, the total number of infected people and deaths reached to 141,801 and 1783, respectively. Bangladesh has strengthened its efforts to improve the health care system’s ability, including COVID-19 diagnosis to prevent the crisis, following discovery of the first 100 reported cases of COVID-19 at the start of April. Though, the government of Bangladesh had put in place preventive measures, the country has no remarkable legislative structures for combating COVID-19 in which Bangladesh, the South Asian low-income economic country, is under very precarious conditions and is at the forefront of the threat of disease that can spread to over the 160 million people. The aim of this article is to describe the current Bangladesh situation as well as the consequences in the country due to COVID-19 and to describe how people are confronted with this pandemic.</p>",
"DOI": "10.5455/jabet.2020.d152",
"views": 741,
"downloads": 133,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/08/178-1592312166-Figure1.jpg",
"caption": "Figure 1. A schematic diagram representing the rapidly evolving COVID-19 situation in Bangladesh.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/08/178-1592312166-Figure2.jpg",
"caption": "Figure 2. The rapid rise in the number of confirmed cases; from March 08, 2020 to June 29, 2020; Bangladesh. Data Source: DGHS [10].",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/08/178-1592312166-Figure3.jpg",
"caption": "Figure 3. Infection and death rates in various age groups and gender; June 29, 2020; Bangladesh. Source: WHO-Bangladesh situation report-19 [12]; Permission ID: 350039.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/08/178-1592312166-Figure4.jpg",
"caption": "Figure 4. The case-doubling period of COVID-19 confirmed cases in all divisions around the country; from the day on which 10 cases were reported, June 29, 2020, Bangladesh. Source: WHO-Bangladesh situation report-19 [12]; Permission ID: 350039.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/08/178-1592312166-Figure5.jpg",
"caption": "Figure 5. The cumulative number of weekly COVID-19 conducted tests and positive rates; from March 08, 2020 to June 29, Bangladesh. Source: WHO-Bangladesh situation report-19 [12]; Permission ID: 350039",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/08/178-1592312166-Figure6.jpg",
"caption": "Figure 6: The number of people in home quarantine and released from quarantine; March 16, 2020 to June 29, 2020, Bangladesh. Source: WHO-Bangladesh situation report-19 [12]; Permission ID: 350039.",
"featured": false
}
],
"authors": [
{
"id": 249,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Fahmida Begum",
"family_name": "Mina",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 73
},
{
"id": 250,
"affiliation": [
{
"affiliation": "Professor Joarder DNA & Chromosome Research Laboratory, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Mutasim",
"family_name": "Billah",
"email": "mutasimbillahshazu@gmail.com",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mutasim Billah, Professor Joarder DNA & Chromosome Research Laboratory, University of Rajshahi, Rajshahi-6205, Bangladesh, E-mail: mutasimbillahshazu@gmail.com",
"article": 73
},
{
"id": 251,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Siddikur",
"family_name": "Rahman",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 73
},
{
"id": 252,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Sabuj",
"family_name": "Das",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 73
},
{
"id": 253,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Sumon",
"family_name": "Karmakar",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 73
},
{
"id": 254,
"affiliation": [
{
"affiliation": "Professor Joarder DNA & Chromosome Research Laboratory, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Faruk",
"family_name": "Hasan",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 73
},
{
"id": 255,
"affiliation": [
{
"affiliation": "Professor Joarder DNA & Chromosome Research Laboratory, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Uzzal Kumar",
"family_name": "Acharjee",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 73
}
]
},
{
"id": 60,
"slug": "178-1592221000-self-confidence-as-an-immune-modifying-psychotherapeutic-intervention-for-covid-19-patients-and-understanding-of-its-connection-to-cns-endocrine-immune-axis",
"type": "editorial_article",
"manuscript_id": "178-1592221000",
"published": "2020-06-16",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/38/178-1592221000.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 14-17.",
"academic_editor": "Md. Masudur Rahman, PhD;\r\nSylhet Agricultural University, Sylhet 3100, Bangladesh.",
"cite_info": "Hannan MA, Islam MN, Uddin MJ. Self-confidence as an immune-modifying psychotherapeutic intervention for COVID-19 patients and understanding of its connection to CNS-endocrine-immune axis. J Adv Biotechnol Exp Ther. 2020; 3(4): 14-17.",
"title": "Self-confidence as an immune-modifying psychotherapeutic intervention for COVID-19 patients and understanding of its connection to CNS-endocrine-immune axis",
"abstract": "",
"DOI": "10.5455/jabet.2020.d151",
"views": 2137,
"downloads": 241,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/19/31/Figure_1.jpg",
"caption": "Figure 1. Possible mechanisms involved in the impact of self-confidence on COVID-19. Developing self-confidence through psychological interventions, social supports, spiritual connections, food habits, health supports, and positive thinking can potentially reduce psychological stress, thereby consolidating immune functions. Besides, self-confidence can help improve non-communicable diseases such as neurodegenerative as well as inflammatory bowel diseases. As a consequence, self-confidence enables an individual to overcome COVID-19 and reduces the risk of contracting the disease. ACTH, adrenocorticotrophic hormone; GH, growth hormone; NK, natural killer, and APCs, antigen presenting cells.",
"featured": true
}
],
"authors": [
{
"id": 197,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea"
},
{
"affiliation": "Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Abdul",
"family_name": "Hannan",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 60
},
{
"id": 198,
"affiliation": [
{
"affiliation": "Department of Neuroscience, Yamaguchi University Graduate School of Medicine, 1-1-1, Minami Kogushi, Ube 755-8505, Japan"
}
],
"first_name": "Md. Nabiul",
"family_name": "Islam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 60
},
{
"id": 199,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": "hasan800920@gmail.com",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-2911-3255",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Jamal Uddin, PhD; \r\nGraduate School of Pharmaceutical Sciences, College\r\nof Pharmacy, Ewha Womans University, Seoul 03760, Republic of Korea\r\nPhone: +821086737008, Email: hasan800920@gmail.com.",
"article": 60
}
]
},
{
"id": 49,
"slug": "178-1590307525-covid-19-the-catastrophe-of-our-time",
"type": "review_article",
"manuscript_id": "178-1590307525",
"published": "2020-06-15",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/58/178-1590307525.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(4): 01-13.",
"academic_editor": "Md. Masudur Rahman, PhD\r\nSylhet Agricultural University\r\nBangladesh.",
"cite_info": "Sheam MM, Syed SB, Barman SK, et al. COVID-19: The catastrophe of our time. J Adv Biotechnol Exp Ther. 2020; 3(4): 01-13.",
"title": "COVID-19: The catastrophe of our time",
"abstract": "<p>The most discussed topic in today’s world is COVID-19, an acute respiratory disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) because of its contagious transmission pattern, and morbimortality. The virus was originated by bats and in December 2019, first spread to humans by unknown intermediate species in Wuhan, China. The dramatic acceleration of the occurrence and death toll of COVID-19 with no potential medicine and vaccine are enough to explain its severity. This review summarizes multidisciplinary aspects of COVID-19, including origin, epidemiology, symptoms, transmission, pathogenicity, impact on world economy and advances in the use of modern diagnostic procedures and methods. Further, we analyzed extensively for various therapeutic strategies, potential drug options with prospective vaccine candidates and challenges along the way. All data were accumulated through extensive study of recent peer-reviewed publications and authentic reports until June 7, 2020. Collectively, this review would help to shed light on different dimensions of this ongoing pandemic.</p>",
"DOI": "10.5455/jabet.2020.d150",
"views": 1253,
"downloads": 143,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/08/178-1590307525-Figure1.jpg",
"caption": "Figure 1. Origin and transmission model of coronavirus.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/56/08/178-1590307525-Figure2.jpg",
"caption": "Figure 2. Simplified presentation of possible therapeutic development sites for SARS-CoV-2. (a) If the entry of SARS-CoV-2 can be inhibited into the host cells, it is possible to avoid its infections. Monoclonal antibodies, convalescent plasma might be useful to inhibit/modify the spike proteins, ACE2 of SARS-CoV-2 [69]. (b) Positive RNA strand of the virus is directly translated by ribosomes of the host cells. The translated polypeptides undergo proteolysis mainly by two proteinases namely, proteinase 3CLpro and papain-like pro-tease Plpro. Hence these two proteinases might be targeted to inhibit peptide processing after translation. Lopinavir-ritonavir might be useful in this regard. (c) RNA-dependent RNA polymerase (RdRP) transcribes RNA strand from the viral RNA utilizing host cellular machineries. Therefore, inhibition or inactivation of this reverse transcriptase enzyme might be a good option from therapeutic developmental views. For example, Remdisivir is a possible candidate for reverse transcriptase inhibition.",
"featured": false
}
],
"authors": [
{
"id": 138,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Md. Moinuddin",
"family_name": "Sheam",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 49
},
{
"id": 139,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Shifath Bin",
"family_name": "Syed",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 49
},
{
"id": 140,
"affiliation": [
{
"affiliation": "School of Science, Western Sydney University, Locked Bag, Penrith NSW, Australia"
}
],
"first_name": "Shital Kumar",
"family_name": "Barman",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 49
},
{
"id": 141,
"affiliation": [
{
"affiliation": "Division of Microbiology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia"
}
],
"first_name": "Md Rockybul",
"family_name": "Hasan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 49
},
{
"id": 142,
"affiliation": [
{
"affiliation": "Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
},
{
"affiliation": "Central Laboratory, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Dipak Kumar",
"family_name": "Paul",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 49
},
{
"id": 143,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
}
],
"first_name": "Rokibul",
"family_name": "Islam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 49
},
{
"id": 144,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"
},
{
"affiliation": "Central Laboratory, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Sudhangshu Kumar",
"family_name": "Biswas",
"email": "shu_genetics@yahoo.com",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-3836-2336",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sudhangshu Kumar Biswas, PhD\r\nDepartment of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh, Email: shu_genetics@yahoo.com",
"article": 49
}
]
}
]
},
{
"id": 12,
"title": "Vol3 Issue3",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2020",
"slug": "2020-vol3-issue3",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 210,
"slug": "178-1593625242-tissue-culture-of-phalaenopsis-present-status-and-future-prospects",
"type": "review_article",
"manuscript_id": "178-1593625242",
"published": "2020-08-16",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/51/178-1593625242.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 273-285.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research Center, Bangladesh.",
"cite_info": "Khatun K, Nath UK, et al. Tissue culture of Phalaenopsis: present status and future prospects. J Adv Biotechnol Exp Ther. 2020; 3(3): 273-285.",
"title": "Tissue culture of Phalaenopsis: present status and future prospects",
"abstract": "<p><em>Phalaenopsis </em>one of the popular cut-flower among the orchid species. The improvement/multiplication of this orchid is very difficult through conventional breeding due to delay flowering and uneven flower characteristics. Therefore, tissue culture techniques have been extensively used for improvement of <em>Phalaenopsis </em>by inducing and selecting somaclonal variants. However, it is difficult to get stable regenerations techniques of <em>Phalaenopsis </em>due to production of phenolic compounds, arising somaclonal variation in the culture and less recovery in the field of the regenerated plantlets. Improved and modified tissue culture techniques providing regeneration from various vegetative parts of plant are needed for industrialization and <em>ex situ</em> conservation of this valuable orchid. In this paper we have reviewed various <em>in vitro</em> propagation methods of <em>Phalaenopsis </em>culture which will be helpful for commercialization of this valuable orchid.</p>",
"DOI": "10.5455/jabet.2020.d135",
"views": 3498,
"downloads": 286,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/34/08/178-1593625242-Figure1.jpg",
"caption": "Figure 1. Schematic diagram of Phalaenopsis propagation method: 1) Phalaenopsis propagation can be done by cutting which may result delay flowering and uneven flower characteristics. 2) Propagation can also be done by tissue culture. Tissue culture of Phalaenopsis is done by using different method e.g. organogenesis and somatic embryogenesis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/34/08/178-1593625242-Figure2.jpg",
"caption": "Figure 2. Direct somatic embryogenesis and Plant regeneration of Phalaenopsis amabilis using leaf explants : (A) somatic embryos after 20 days of culture (bar = 700 mm); (B) enlarged and elongated embryos after 30 days of culture (bar = 750 mm); (C) green embryos under light and young somatic protocorm after 45 days of culture (bar = 850 mm); (D) somatic embryos developed from leaf-derived nodular masses (bar = 950 mm); (E) shoots and some formed secondary embryos from the developed embryos (bar = 1.2 mm); (F) leaf-derived embryos formed shoots (bar = 2 mm);(G) a plantlet from the leaf derived embryos (bar = 7.2 mm) [62].",
"featured": false
}
],
"authors": [
{
"id": 933,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh"
}
],
"first_name": "Khadiza",
"family_name": "Khatun",
"email": "kkr@pstu.ac.bd",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Khadiza Khatun, Department of Biotechnology, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh, Phone: +8801763405303, Email: kkr@pstu.ac.bd",
"article": 210
},
{
"id": 934,
"affiliation": [
{
"affiliation": "Bangladesh Argicultural University, Mymensingh, Bangladesh"
}
],
"first_name": "Ujjal Kumar",
"family_name": "Nath",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 210
},
{
"id": 935,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh"
}
],
"first_name": "Md. Shafikur",
"family_name": "Rahman",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 210
}
]
},
{
"id": 209,
"slug": "178-1593501943-molecular-identification-of-four-medicinal-plants-using-dna-barcoding-approach-from-chittagong-bangladesh",
"type": "original_article",
"manuscript_id": "178-1593501943",
"published": "2020-08-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/23/178-1593501943.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 268-272.",
"academic_editor": "Dr. Shahed Uddin Ahmed Shazib, Smith College & University, Massachusetts, USA.",
"cite_info": "Amin S, Ghosh S, et al. Molecular identification of four medicinal plants using DNA barcoding approach from Chittagong, Bangladesh. J Adv Biotechnol Exp Ther. 2020; 3(3): 268-272.",
"title": "Molecular identification of four medicinal plants using DNA barcoding approach from Chittagong, Bangladesh",
"abstract": "<p>Accurate identification of important plants is essential for their safety, efficacy and herbal remedies. The study was aimed to identify 4 locally available medicinal plants using DNA barcoding approach. Genomic DNA was extracted from plant samples followed by their amplification by the conventional PCR approach. Short sequence diversity of standardized specific coding gene regions of <em>matK</em> gene of plastid genome was used to compare and differentiate the plant species. Subsequently, all the samples were purified and sequenced successfully. A phylogenetic tree was constructed to assess their cross-species relationship. All the samples showed a high similarity rate with their homologs after blasting them in NCBI database. The phylogenetic study showed a distinguished relationship with each other. All the result indicates that DNA barcoding approach could be successfully used for reliable identification of medicinal plants and <em>matK</em> gene is a good candidate for this approach.</p>",
"DOI": "10.5455/jabet.2020.d134",
"views": 2040,
"downloads": 244,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/08/178-1593501943-Figure1.jpg",
"caption": "Figure 1. Physical appearance of nine medicinal plants used in this study. a) Azadirachta indica, b) Justicia adhatoda, c) Calotropis procera, d) Mikania scandens.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/08/178-1593501943-Figure2.jpg",
"caption": "Figure 2. Evolutionary relationships of taxa.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/47/08/178-1593501943-Figure3.jpg",
"caption": "Figure 3. Partially amplified matK sequences by one pair of barcode primer by PCR on 1% agarose gel. Lane 1: 100 bp DNA ladder; Lane 2: matK genome of Azadirachta indica; Lane 3: matK genome of Mikania scandens; Lane 4: matK genome of Calotropis procera.",
"featured": false
}
],
"authors": [
{
"id": 927,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, Faculty of Basic Medical and Pharmaceutical Sciences, University of Science and Technology Chittagong (USTC), Foy’s Lake, Khulshi-4202, Chittagong, Bangladesh."
}
],
"first_name": "Sabrina",
"family_name": "Amin",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 209
},
{
"id": 928,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, Faculty of Basic Medical and Pharmaceutical Sciences, University of Science and Technology Chittagong (USTC), Foy’s Lake, Khulshi-4202, Chittagong, Bangladesh."
}
],
"first_name": "Srabasti",
"family_name": "Ghosh",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 209
},
{
"id": 929,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, Faculty of Basic Medical and Pharmaceutical Sciences, University of Science and Technology Chittagong (USTC), Foy’s Lake, Khulshi-4202, Chittagong, Bangladesh."
}
],
"first_name": "Baishakhi",
"family_name": "Biswas",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 209
},
{
"id": 930,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, Faculty of Basic Medical and Pharmaceutical Sciences, University of Science and Technology Chittagong (USTC), Foy’s Lake, Khulshi-4202, Chittagong, Bangladesh."
}
],
"first_name": "Md.",
"family_name": "Arifuzzaman",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 209
},
{
"id": 931,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, Faculty of Basic Medical and Pharmaceutical Sciences, University of Science and Technology Chittagong (USTC), Foy’s Lake, Khulshi-4202, Chittagong, Bangladesh."
}
],
"first_name": "Md. Abul Kalam",
"family_name": "Azad",
"email": "akazadbtech@gmail.com",
"author_order": 5,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Abul Kalam Azad, Department of Biochemistry and Biotechnology, Faculty of Basic Medical and Pharmaceutical Sciences, University of Science and Technology Chittagong (USTC), Foy’s Lake, Khulshi-4202, Chittagong, Bangladesh. Phone: +8801836930655, Email: akazadbtech@gmail.com",
"article": 209
},
{
"id": 932,
"affiliation": [
{
"affiliation": "Genomics Research Group, Chittagong Veterinary and Animal Science University, Khulshi -4225, Chittagong, Bangladesh."
},
{
"affiliation": "Nextgen Informatics Pvt Ltd."
}
],
"first_name": "AMAM Zonaed",
"family_name": "Siddiki",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 209
}
]
},
{
"id": 208,
"slug": "178-1592098521-prophylactic-effect-of-vitamin-e-and-coriander-coriandrum-sativum-seed-extract-against-lead-toxicity-in-liver-of-swiss-albino-mice",
"type": "original_article",
"manuscript_id": "178-1592098521",
"published": "2020-08-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/29/178-1592098521.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 263-267.",
"academic_editor": "Dr. Md. Abdul Hannan, Dongguk University, South Korea.",
"cite_info": "Jahid MA, Khan MZI, et al. Prophylactic effect of vitamin E and coriander (Coriandrum sativum) seed extract against lead toxicity in liver of Swiss albino mice. J Adv Biotechnol Exp Ther. 2020; 3(3): 263-267.",
"title": "Prophylactic effect of vitamin E and coriander (Coriandrum sativum) seed extract against lead toxicity in liver of Swiss albino mice",
"abstract": "<p>Lead is the most abundant toxic metal in the environment that causes serious health hazards in animals and humans. In this study, the prophylactic effects of vitamin E and coriander (<em>Coriandrum sativum </em>L.) seed extract on lead-induced liver damage in Swiss albino mice were investigated by gross and histological studies. At first, mice were intoxicated with a particular dose of lead acetate for 42 days. After intoxication, mice were divided into three groups for treatment purpose. The first and second groups of mice were treated with vitamin E and coriander extract respectively. Both vitamin E and coriander extract were given to the third group of mice. Treatment was done for 42 days. In this study, lead acetate was found to cause nodular lesion and congestion in the central vein in liver. Vitamin E was found effective in the treatment of congestion in the central vein of liver but ineffective in the treatment of the nodular lesion. The nodular lesion was found in liver even after vitamin E treatment. The liver was found normal in the coriander extract-treated group as well as combined vitamin E and coriander extract-treated group. There was no nodular lesion as well as congestion in the central vein of liver in these two groups. The present findings revealed that lead has detrimental effects on the liver of mice. Treatment with coriander extract was found more effective than the treatment with vitamin E in lead intoxicated mice. The combined action of vitamin E and coriander extract is more effective than their individual action. The present investigation may serve as baseline data about the adverse effects of lead toxicity and efficacy of vitamin E and coriander extract against lead toxicity.</p>",
"DOI": "10.5455/jabet.2020.d133",
"views": 811,
"downloads": 128,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/59/08/178-1592098521-Figure1.jpg",
"caption": "Figure 1. Gross observation of liver in mice. Normal appearance of liver was found in the control group (A). Nodular lesion (white arrow) was found in the lead intoxicated group (B). Nodular lesion (white arrow) was also found in the vitamin E treated group (C). Normal appearance of liver in coriander extract-treated group (D), and vitamin E and coriander extract (combined) treated group (E). Scale bar = 1 cm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/59/08/178-1592098521-Figure2.jpg",
"caption": "Figure 2. Weight (A) and length (B) of liver of mice in different groups (Mean ± standard error).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/59/08/178-1592098521-Figure3.jpg",
"caption": "Figure 3. (A-F): Histological observation of liver in mice (H&E). Normal appearance of liver was found in the control group (A, 10X and 40X). In the lead intoxicated group, congestion in the central vein (white arrow) was found in some sections (B, 10X and 40X). In addition, nodular lesion (blue arrow) was also found in some sections of this group (C, 10X and 40X). Appearance of liver was found normal in vitamin E treated group (D, 10X and 40X), coriander extract-treated group (E, 10X and 40X), and vitamin E and coriander extract (combined) treated group (F, 10X and 40X). CV= Central vein. Scale bar: 5 μm (10X) and 1 μm (40X).",
"featured": false
}
],
"authors": [
{
"id": 924,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Khanpura, Babugonj, Barishal-8210, Bangladesh."
}
],
"first_name": "Md. Anwar",
"family_name": "Jahid",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 208
},
{
"id": 925,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Mohammad Zahirul Islam",
"family_name": "Khan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 208
},
{
"id": 926,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Mohammad Rafiqul",
"family_name": "Islam",
"email": "rafiqul.islam@bau.edu.bd",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Mohammad Rafiqul Islam, Department of Anatomy & Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, E-mail: rafiqul.islam@bau.edu.bd",
"article": 208
}
]
},
{
"id": 205,
"slug": "178-1594026646-isolation-and-characterization-of-bacteria-from-two-soil-samples-and-their-effect-on-wheat-triticum-aestivum-l-growth-promotion",
"type": "original_article",
"manuscript_id": "178-1594026646",
"published": "2020-07-28",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/30/178-1594026646.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 254-262.",
"academic_editor": "Dr. Hasan-Al-Faruque, Daegu Gyeongbuk Institute of Science & Technology, South Korea.",
"cite_info": "Paul GK, Mahmud S, et al. Isolation and characterization of bacteria from two soil samples and their effect on wheat (Triticum aestivum L.) growth promotion. J Adv Biotechnol Exp Ther. 2020; 3(3): 254-262.",
"title": "Isolation and characterization of bacteria from two soil samples and their effect on wheat (Triticum aestivum L.) growth promotion",
"abstract": "<p>Since ancient times, soil bacteria play an important role on crop growth and yield by genetic transformation naturally. But the continuous use of chemical fertilizers reduces their number and proper environment for multiplication. Seed treatment with beneficial bacteria provides nutrients for the growth of crop plants. Thus, soil bacteria were isolated, their growth characteristics and effect on wheat growth were observed. The maximum growth of Isolate A and Isolate B was observed at pH 5.5, 7.0 and 33°C, 35°C respectively. Morphological characteristics indicated that Isolate A and Isolate B were gram-positive. But both bacteria were non-motile. In Biochemical test, both of them showed positive result in the methyl red test, catalase test, urea test, starch hydrolysis test, and negative in TSI (Triple Sugar Iron) test, mannitol salt test. Isolate B showed positive result in BSA, MacConkey test and EMB (Eosin Methylene Blue) test and Isolate A showed negative result in BSA (Bismuth Sulphite Agar), MacConkey test and EMB test. Besides, both of the bacteria were multi-drug resistance showing resistance to penicillin, amoxicillin, ampicillin cefuroxime, and ceftazidime.16S rRNA gene sequencing identified the isolate A and Isolate B as <em>Bacillus thuringiensis</em> and <em>Bacillus anthracis</em>. After 6 hours of wheat seed treatment germination percentage, fresh root and shoot weight, root and shoot dry weight, relative water content of both root and shoot, and plant growth was enhanced by <em>Bacillus thuringiensis</em> and <em>Bacillus anthracis</em>. <em>Bacillus anthracis</em> was more capable than <em>bacillus thuringiensis</em> for increasing germination rates, both root and shoot growth of wheat. It indicated that <em>Bacillus anthracis</em> and <em>Bacillus thuringiensis</em> mediated growth improvement of wheat is possibly originated in roots.</p>",
"DOI": "10.5455/jabet.2020.d132",
"views": 1622,
"downloads": 182,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/08/178-1594026646-Figure1.jpg",
"caption": "Figure 1. Streaking and pure LB agar plate of Isolate A and Isolate B bacterial strain.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/08/178-1594026646-Figure2.jpg",
"caption": "Figure 2. Antibiotic sensitivity test result of Isolate A and isolate B.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/08/178-1594026646-Figure3.jpg",
"caption": "Figure 3. Effect of pH and temperature on the growth of Isolate A and Isolate B bacteria after 24 hours of incubation. Error bars presented mean ± standard deviation of triplicates of three independent experiments.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/08/178-1594026646-Figure4.jpg",
"caption": "Figure 4. Germination percentage of both treated and untreated wheat seeds in different time duration. Different letters indicate significant differences between mean ±SD of treatments (n=3) at p<0.05 significance level.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/08/178-1594026646-Figure5.jpg",
"caption": "Figure 5. Morphological characteristics of wheat seedlings treated with Bacillus thuringiensis and Bacillus anthracis. Shoot length of wheat seedlings (A), Root length of wheat seedlings (B), Fresh shoot weight (C), Fresh root weight (D), Shoot dry weight (E), Root dry weight (F), Relative water content in shoot (G) and Relative water content in root (H). Different letters indicate significant differences between mean ±SD of treatments (n=3) at p<0.05 significance level.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/13/08/178-1594026646-Figure6.jpg",
"caption": "Figure 6. Schematic diagram of summary.",
"featured": false
}
],
"authors": [
{
"id": 905,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Gobindo Kumar",
"family_name": "Paul",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 205
},
{
"id": 906,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Shafi",
"family_name": "Mahmud",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 205
},
{
"id": 907,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Kamrun",
"family_name": "Naher",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 205
},
{
"id": 908,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Tabassum",
"family_name": "Jabin",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 205
},
{
"id": 909,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Md. Liton",
"family_name": "Mahmud",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 205
},
{
"id": 910,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Md. Nazmul",
"family_name": "Haque",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 205
},
{
"id": 911,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Md. Salah",
"family_name": "Uddin",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 205
},
{
"id": 912,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Shahriar",
"family_name": "Zaman",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 205
},
{
"id": 913,
"affiliation": [
{
"affiliation": "Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Md. Abu",
"family_name": "Saleh",
"email": "saleh@ru.ac.bd",
"author_order": 9,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Md. Abu Saleh, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh, E-mail: saleh@ru.ac.bd",
"article": 205
}
]
},
{
"id": 204,
"slug": "178-1593668249-tyrosine-phosphorylation-related-to-thymic-involution-induced-by-diet-restriction-in-comparison-with-aging",
"type": "original_article",
"manuscript_id": "178-1593668249",
"published": "2020-07-21",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/04/178-1593668249.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 248-253.",
"academic_editor": "Dr. Md. Jamal Uddin, Ewha Womans University, South Korea.",
"cite_info": "Takase I. Tyrosine phosphorylation related to thymic involution induced by diet restriction in comparison with aging. J Adv Biotechnol Exp Ther. 2020; 3(3): 248-253.",
"title": "Tyrosine phosphorylation related to thymic involution induced by diet restriction in comparison with aging",
"abstract": "<p>Tyrosine phosphorylation signaling is known to be essential for the proliferation and differentiation of cells. Protein tyrosine kinases (PTKs) are present mainly in the lymphoid tissues including the thymus. The objective of the study was to investigate the expression of PTKs associated with stress-related thymic involution due to diet restriction compared with that due to aging. p56<sup>lck</sup> and p59<sup>f</sup><sup>yn</sup> belong to the Src family membrane-associated PTKs. We found that diet-restricted rats had significantly lower thymus weights, and the expression of p59<sup>fyn</sup> was significantly decreased compared with the control group. In contrast, the expression of p56<sup>lck </sup>was not significantly different. We also found that aging-related thymic involution was not affected by the expression of those kinases. We confirmed that the mechanisms of diet-restricted thymic involution were different from those of aging-related thymic involution. It might be used as an index of chronic stress due to diet restriction in cases of child abuse or neglect.</p>",
"DOI": "10.5455/jabet.2020.d131",
"views": 170,
"downloads": 106,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/14/178-1593668249-Figure1.jpg",
"caption": "Figure 1. The differences of body weights, thymus weights and thymus/body weight ratios between the control group and the stressed group in the diet restriction model compared to each control set as 100% (***p<0.001, **p<0.01).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/14/178-1593668249-Figure2.jpg",
"caption": "Figure 2. Representative Western blots of 4G10 (A), p59fyn (B) and p56lck (C) in the diet restriction model. Numerical digits at the bottom of each figure showed the intensity of each band compared to that of each control group set as 100 using Image J (NIH, USA).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/14/178-1593668249-Figure3.jpg",
"caption": "Figure 3. The changes of thymus/body weight ratios of the 3-week-old group, the 8-week-old group and the aged group in the aging model (***p<0.001, **p<0.01) demonstrated the phenomenon called ‘physiologic thymic involution (or atrophy)’.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/14/178-1593668249-Figure4.jpg",
"caption": "Figure 4. Representative Western blots of 4G10 (A), p59fyn (B) and p56lck (C) of the 3-week-old group, the 8-week-old group and the aged group in the aging model.",
"featured": false
}
],
"authors": [
{
"id": 904,
"affiliation": [
{
"affiliation": "Department of Legal Medicine, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube City, Yamaguchi 755-8505, Japan"
}
],
"first_name": "Izumi",
"family_name": "Takase",
"email": "takase@yamaguchi-u.ac.jp",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Izumi Takase, 1Department of Legal Medicine, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami\u0002Kogushi, Ube City, Yamaguchi 755-8505, Japan, e-mail: takase@yamaguchi-u.ac.jp",
"article": 204
}
]
},
{
"id": 203,
"slug": "178-1591686107-dietary-acidifier-and-lysozyme-improve-growth-performances-and-hemato-biochemical-profile-in-broiler-chicken",
"type": "original_article",
"manuscript_id": "178-1591686107",
"published": "2020-07-12",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/29/178-1591686107.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 241-247.",
"academic_editor": "Dr. Md Nabiul Islam, Yamaguchi University, Japan.",
"cite_info": "Khalil KKI, Islam MA, et al. Dietary acidifier and lysozyme improve growth performances and hemato-biochemical profile in broiler chicken. J Adv Biotechnol Exp Ther. 2020; 3(3): 241-247.",
"title": "Dietary acidifier and lysozyme improve growth performances and hemato-biochemical profile in broiler chicken",
"abstract": "<p><strong> </strong>Acidifier and lysozyme may have a significant impact on growth performance and body defense in broilers. The experiment was conducted to evaluate the effects of dietary acidifier and lysozyme on physiognomies (live body weight and body weight gain), feed conversion ratio (FCR) and selected hemato-biochemical parameters in broiler chicken. A total of 60, day old Lohman broiler chicks were collected and reared up to 28 days with timely vaccination. At day 14, the chicks were randomly divided into four equal groups: A, B, C and D (n=15). Group A was considered as non-treated control; group B was supplemented with acidifier, group C with lysozyme and group D with both acidifier and lysozyme through drinking water. Results showed that acidifier, lysozyme and combined groups had significantly higher live body weight and lower feed conversion ratio, FCR (p<0.05) compared to the control group. Total erythrocyte count (TEC), hemoglobin concentration and packed cell volume (PCV) were significantly higher (p<0.05) especially in lysozyme supplemented group. Serum lipid profile (total cholesterol, triglyceride and LDL cholesterol) were significantly decreased in the acidifier supplemented group, whereas HDL cholesterol was increased (p<0.05). Lysozyme supplemented birds showed an increased level of total cholesterol and significant (p<0.05) decrease in triglyceride and LDL level. Combined supplementation showed almost similar results as lysozyme. Serum alanine transaminase (ALT), aspartate transaminase (AST) and creatinine concentration didn’t differ significantly upon supplementations. It is concluded that acidifier and lysozyme could be used in broiler feed for better growth performance and hemato-biochemical profile.</p>",
"DOI": "10.5455/jabet.2020.d130",
"views": 909,
"downloads": 169,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/52/14/178-1591686107-Figure1.jpg",
"caption": "Figure 1. Effects of dietary acidifier and lysozyme on physiognomies (mean ± SEM) in broiler chicken at 28th day of age. Broilers birds were reared with supplemented acidifier, lysozyme and both (details in materials and methods). A, body weight; B, body weight gain; C, percentage of body weight gain; D, feed conversion ratio (FCR). Different letters: a,b and c over a bar graph denote significant difference at p<0.05.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/52/14/178-1591686107-Figure2.jpg",
"caption": "Figure 2. Effects of dietary acidifier and lysozyme on lipid profile (mean ± SEM) in broiler chicken at 28th day of age. Broilers birds were reared with supplemented acidifier, lysozyme and both (see Materials and Methods for details). Blood samples were collected, and sera were separated and analysed for lipid profile. A, TC, total cholesterol; B, TG, triglycerides; C, LDL-c, low density lipoprotein cholesterol and D, HDL-c, high density lipoprotein cholesterol. Different letters: a,b, and cover a bar graph denote significant difference among the group at p<0.05 level.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/52/14/178-1591686107-Figure3.jpg",
"caption": "Figure 3. Effects of dietary acidifier and lysozyme on ALT, AST and creatinine (mean ± SEM) in broiler chicken of the experimental groups. Broilers birds were reared with supplemented acidifier, lysozyme and both (see Materials and Methods for details). Blood samples were collected, and sera were separated and analyzed. A, AST, aspartate amino transferase; B, ALT, alanine amino transferase; and C, creatinine. ns - Not significant (p>0.05)",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/52/14/178-1591686107-Figure4.jpg",
"caption": "Figure 4. Schematic view of the experiment revealing promoting effects of dietary acidifier and lysozymes in broiler chickens.",
"featured": false
}
],
"authors": [
{
"id": 898,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202."
}
],
"first_name": "Kazi Khalid Ibne",
"family_name": "Khalil",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 203
},
{
"id": 899,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202."
}
],
"first_name": "Md. Atiqul",
"family_name": "Islam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 203
},
{
"id": 900,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202."
}
],
"first_name": "Khaled Mahmud",
"family_name": "Sujan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 203
},
{
"id": 901,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202."
}
],
"first_name": "Afrina",
"family_name": "Mustari",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 203
},
{
"id": 902,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202."
}
],
"first_name": "Nazim",
"family_name": "Ahmad",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 203
},
{
"id": 903,
"affiliation": [
{
"affiliation": "Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202."
}
],
"first_name": "Mohammad Alam",
"family_name": "Miah",
"email": "mam74@bau.edu.bd",
"author_order": 6,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Mohammad Alam Miah, Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202. Email: mam74@bau.edu.bd",
"article": 203
}
]
},
{
"id": 202,
"slug": "178-1589274220-prediction-of-immune-pressure-on-hiv-1-regulatory-gene-tat-by-human-host-through-bioinformatics-tools",
"type": "original_article",
"manuscript_id": "178-1589274220",
"published": "2020-06-27",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/55/178-1589274220.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 233-240.",
"academic_editor": "Dr. Md. Abdul Hannan, Dongguk University, South Korea.",
"cite_info": "Hasan Z, Hasan M, et al. Prediction of immune pressure on HIV-1 regulatory gene tat by human host through bioinformatics tools. J Adv Biotechnol Exp Ther. 2020; 3(3): 233-240.",
"title": "Prediction of immune pressure on HIV-1 regulatory gene tat by human host through bioinformatics tools",
"abstract": "<p>In the early establishment of HIV-1 infection, Tat protein plays an essential role in controlling other genes of HIV-1 (e.g., vif, vpr, vpu, nef, gag, pol and env), for viral pathogenesis, while maintaining its polymorphic nature. It is well documented that polymorphism of HIV-1 genome are created to escape immune pressures (e.g. CD8+, CD4+, B, NK-cells and others) by human host, during the course of infection. Over the time those mutations are incorporated or left in the HIV genome as an escaped flag or signature amino acid, and this scenario could be predictable using contemporary bioinformatics tools. Our sequence analysis from global database (LANL) revealed that Tat protein under positive immune pressure as dn/ds >1.5, even though differential immune pressure exists among the HIV-1 subtype. Average entropy score is 0.31, implying the less variable nature of this protein while amino acid variations are higher in C-terminal. Remarkably, the region encompassing by positions 38 to 51 amino acids are relatively conserved, over the year 2009 to 2017 across HIV-1 subtype. Indeed, subtype-specific SNP or signature amino acids were observed in various position of Tat, dominantly in C-terminal end. Our epitope density plot analysis, highlighted CTLs/CD8+ cells play a major role on Tat sequence variation around the globe. Taken together, our analysis illustrated the dynamic nature of polymorphism within HIV-1 proteins, which could be predictable to see the immune-mediated selective pressure by human host on viral genome.</p>",
"DOI": "10.5455/jabet.2020.d129",
"views": 631,
"downloads": 97,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/14/178-1589274220-Figure1.jpg",
"caption": "Figure 1. dn/ds ratio among HIV-1 subtype from 2009 to 2017. Autologous sequences of HIV-1 subtype A, B, C, D, F, G, H, AE, AG, BC and Cpx were retrieved from Los Alamos National Laboratory (LANL). (A) dn/ds ratio was calculated among the clades of HIV-1, (B) average dn/ds ratio was compared among the years from 2009 to 2017, (C) comparison of dn/ds ratio between clade B and C over the year 2009 to 2017 which is statistically significant using student's t-test (p< 0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/14/178-1589274220-Figure2.jpg",
"caption": "Figure 2. Entropy variation of HIV-1 group M from 2009 to 2017. (A) Shannon entropy was used to measure the relative variation in different positions or regions of an aligned protein of Tat from 2009 to 2017. (B) comparison of entropy level over the years of HIV-1 group M Tat protein.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/14/178-1589274220-Figure3.jpg",
"caption": "Figure 3. Sequence alignment of Tat protein of HIV-1 subtypes. Consensus Tat protein sequences of different subtypes (A, B, C, D, F, G, H, AE, AG, BC and Cpx) were aligned with consensus HIV-1 M group from 2004 using LANL database.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/14/178-1589274220-Figure4.jpg",
"caption": "Figure 4. Epitope density plots across Tat protein of HIV-1. Density plot of reported epitopes from the literature on Tat protein of HIV-1. Data were adapted from HIV molecular immunology database of LANL",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/14/178-1589274220-Figure5.jpg",
"caption": "Figure 5. Flow diagram of entire steps. Graphical representation of complete processes which has been taken in this study are summarized.",
"featured": false
}
],
"authors": [
{
"id": 890,
"affiliation": [
{
"affiliation": "Dept. of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh."
}
],
"first_name": "Zafrul",
"family_name": "Hasan",
"email": "zafrul-bmb@sust.edu",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Zafrul Hasan, Dept. of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh. E-mail: zafrul-bmb@sust.edu",
"article": 202
},
{
"id": 891,
"affiliation": [
{
"affiliation": "Dept. of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh."
}
],
"first_name": "Mahedi",
"family_name": "Hasan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 202
},
{
"id": 892,
"affiliation": [
{
"affiliation": "Dept. of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh."
}
],
"first_name": "Arafat Islam",
"family_name": "Ashik",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 202
},
{
"id": 893,
"affiliation": [
{
"affiliation": "Dept. of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh."
}
],
"first_name": "Rumana",
"family_name": "Mahtarin",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 202
},
{
"id": 894,
"affiliation": [
{
"affiliation": "Dept. of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh."
}
],
"first_name": "Md. Ali",
"family_name": "Newaj",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 202
},
{
"id": 895,
"affiliation": [
{
"affiliation": "Dept. of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh."
}
],
"first_name": "Zakia Sultana",
"family_name": "Nishat",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 202
},
{
"id": 896,
"affiliation": [
{
"affiliation": "Dept. of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh."
}
],
"first_name": "Mohammad Abul",
"family_name": "Hasnat",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 202
},
{
"id": 897,
"affiliation": [
{
"affiliation": "Dept. of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh."
}
],
"first_name": "Md. Waseque",
"family_name": "Mia",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 202
}
]
},
{
"id": 201,
"slug": "178-1590167174-micropropagation-of-commercially-important-orchid-dendrobium-palpebrae-lindl-through-in-vitro-developed-pseudobulb-culture",
"type": "original_article",
"manuscript_id": "178-1590167174",
"published": "2020-06-26",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/03/178-1590167174.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 225-232.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research Center, Bangladesh.",
"cite_info": "Bhowmik TK, Rahman MM. Micropropagation of commercially important orchid Dendrobium palpebrae Lindl. through in vitro developed pseudobulb culture. J Adv Biotechnol Exp Ther. 2020; 3(3): 225-232.",
"title": "Micropropagation of commercially important orchid Dendrobium palpebrae Lindl. through in vitro developed pseudobulb culture",
"abstract": "<p><strong> </strong><em>Dendrobium palpebrae </em>Lindl. an epiphytic fragrant indigenous orchid species of Bangladesh and distributed in Bangladesh, eastern Himalayas, Myanmar, Thailand, Laos and Vietnam at altitudes of 800 to 2500 meters. From <em>in vitro</em> growing plantlets, upper and lower part of pseudobulb segments were aseptically cultured on MS medium supplemented with auxins (IAA, IBA, NAA, Picloram) and cytokinins (BAP, Kinetin). <em>In vitro </em>grown pseudobulb segments both upper and lower part directly produced multiple shoot buds <em>via</em> organogenesis. Average highest number of MSBs (8.21 ± 0.44/segment in lower part; 6.43 ± 0.40/segment in upper part) produced on MS medium with 1.0 mg/l NAA + 2.0 mg/l BAP followed by (7.24 ± 0.41/segment in lower part; 5.96 ± 0.37/segment in upper part) MS medium supplemented with 1.0 mg/l Picloram + 2.0 mg/l BAP. Highest individual shoot bud increased length was recorded on agar solidified MS medium with 1.0 mg/l Picloram + 1.0 mg/l BAP (3.76 ± 0.14 cm) followed by liquid MS + 0.5 mg/l NAA + 1.0 mg/l BAP (3.11 ± 0.12 cm) medium. Elongation of shoot bud was better in agar solidified media than liquid condition and MS was found better than PM. Shoot bud derived seedlings were best responses as increase in length as well as the number of roots developed on agar solidified MS with 0.5 mg/l NAA (4.82 ± 0.22 cm/shoot bud and 2.75 ± 0.17 no/shoot bud) followed by MS with 0.5 mg/l IBA supplemented medium (4.49 ± 0.12 cm/shoot bud and 2.57 ± 0.08 no/shoot bud). The plantlets were successfully transferred to outside environment by successive phases of acclimatization.</p>",
"DOI": "10.5455/jabet.2020.d128",
"views": 1088,
"downloads": 122,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/37/14/178-1590167174-Figure1.jpg",
"caption": "Figure 1. (1a-1f): In vitro micropropagation, shoot bud elongation, rooting, SPSs development and hardening of Dendrobium palpebrae Lindl.",
"featured": false
}
],
"authors": [
{
"id": 888,
"affiliation": [
{
"affiliation": "Department of Botany, University of Chittagong, Chittagong-4331, Bangladesh."
}
],
"first_name": "Tapash Kumar",
"family_name": "Bhowmik",
"email": "tapashptc@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Tapash Kumar Bhowmik, Department of Botany, University of Chittagong, Chittagong-4331, Bangladesh. Email: tapashptc@gmail.com",
"article": 201
},
{
"id": 889,
"affiliation": [
{
"affiliation": "Department of Botany, University of Chittagong, Chittagong-4331, Bangladesh."
}
],
"first_name": "Md. Mahbubur",
"family_name": "Rahman",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 201
}
]
},
{
"id": 200,
"slug": "178-1590260727-biological-investigations-of-the-methanol-extract-of-tetrastigma-leucostaphylum-dennst-alston-ex-mabb-vitaceae-in-vivo-and-in-vitro-approach",
"type": "original_article",
"manuscript_id": "178-1590260727",
"published": "2020-06-14",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/31/178-1590260727.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 216-224.",
"academic_editor": "Dr. Masud Parvez, Washington State University, USA.",
"cite_info": "Rudra S, Sawon MSU, et al. Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach. J Adv Biotechnol Exp Ther. 2020; 3(3): 216-224.",
"title": "Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach",
"abstract": "<p><em>Tetrastigma leucostaphylum </em>(Family: Vitaceae) is popular for its medicinal value in Bangladeshi tribal communities. This study aims to investigate several pharmacological values of methanol extract of <em>T. leucostaphylum</em> (METL). <em>In vivo</em> analgesic and anti-inflammatory researches have been implemented by using acetic acid-induced writhing and formalin-induced paw licking test protocols in mice. Furthermore, <em>in vitro</em> thrombolytic and anthelmintic studies have been performed by following the blood clot lysis method and nematode mortality measurement method. In the <em>in vivo</em> study, METL did not minimize the acetic acid-induced writhes prominently but significantly attenuate both the peripheral and inflammatory pain in mice in a dose-dependent manner. In early and late phase, METL 400 (mg/kg, b.w; p.o) showed 39.63 % and 48.73 % paw licking inhibition. Again, METL (100 µL) reflected 56.62 % clot lysis in thrombolytic research. Besides, METL causes the death of nematodes in a dose-dependent manner. The bioassay of the methanol extract of <em>T. leucostaphylum</em> justified the analgesic, anti-inflammatory, thrombolytic and anthelmintic activities of the crude extract and finally suggests the test extract as a wellspring of anti-inflammatory, thrombolytic and anthelmintic agents as a crude drug source.</p>",
"DOI": "10.5455/jabet.2020.d127",
"views": 591,
"downloads": 68,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/14/178-1590260727-Figure1.jpg",
"caption": "Figure 1. Effect of test samples on the anti-inflammatory investigation of the METL on the formalin-induced licking test. The values were presented as mean ± SEM; One-way analysis of variance (ANOVA) was followed by Dunnett’s test. *P < 0.05, **P < 0.01 and ***P < 0.001, where # was designated as control. METL = methanol extract of Tetrastigma leucostaphylum leaves, TWN 80 = 1% Tween 80, and IBU = Ibuprofen.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/14/178-1590260727-Figure2.jpg",
"caption": "Figure 2. Clot lysis effects by Saline water, Streptokinase, and METL. The values were presented as mean ± SEM (n = 10); One-way analysis of variance (ANOVA) was followed by Dunnett’s test. ***P < 0.001 was considered as significant compared with the control, where # is designated as control. METL = methanol extract of Tetrastigma leucostaphylum leaves, SW = saline water, and STK = streptokinase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/14/178-1590260727-Figure3.jpg",
"caption": "Figure 3. Effect of various test doses of the METL and LMS on nematodes (n = 6) in the anthelmintic study. Values were presented as mean ± SEM. METL = methanol extract of Tetrastigma leucostaphylum leaves, and LMS = levamisole.",
"featured": false
}
],
"authors": [
{
"id": 876,
"affiliation": [
{
"affiliation": "Department of Botany, Faculty of Biological Science, University of Chittagong, Chittagong - 4331, Bangladesh"
}
],
"first_name": "Sajib",
"family_name": "Rudra",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 877,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh"
}
],
"first_name": "Md. Solaman Uddin",
"family_name": "Sawon",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 878,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh"
}
],
"first_name": "Nazim Uddin",
"family_name": "Emon",
"email": "nazim7emon@gmail.com",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0001-7567-4796",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nazim Uddin Emon, Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh, E-mail: nazim7emon@gmail.com",
"article": 200
},
{
"id": 879,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh."
}
],
"first_name": "Safaet",
"family_name": "Alam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 880,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh"
}
],
"first_name": "Syed Mohammed",
"family_name": "Tareq",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 881,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh"
}
],
"first_name": "Mohammad Nazmul",
"family_name": "Islam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 882,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh"
}
],
"first_name": "Mir Md. Rokib",
"family_name": "Uddin",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 883,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh"
}
],
"first_name": "Abdullah Md Sazid",
"family_name": "Chowdhury",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 884,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh"
}
],
"first_name": "Abu Nazer",
"family_name": "Hasbe",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 885,
"affiliation": [
{
"affiliation": "Department of Botany, Faculty of Biological Science, University of Chittagong, Chittagong - 4331, Bangladesh"
}
],
"first_name": "Mohammad",
"family_name": "Shakil",
"email": null,
"author_order": 10,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 886,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh"
}
],
"first_name": "Shahenur Alam",
"family_name": "Sakib",
"email": null,
"author_order": 11,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
},
{
"id": 887,
"affiliation": [
{
"affiliation": "Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh"
}
],
"first_name": "Mohammed Aktar",
"family_name": "Sayeed",
"email": null,
"author_order": 12,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 200
}
]
},
{
"id": 196,
"slug": "178-1582884845-habit-and-habitual-status-with-relative-diversity-study-of-avifauna-of-jaipurhat-district-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1582884845",
"published": "2020-06-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/42/178-1582884845.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 204-215.",
"academic_editor": "Dr. Dinh-Toi Chu, Hanoi National University of Education, Vietnam.",
"cite_info": "Amin A, Hasan K, et al. Habit and habitual status with relative diversity study of avifauna of Jaipurhat district of Bangladesh. J Adv Biotechnol Exp Ther. 2020; 3(3): 204-215.",
"title": "Habit and habitual status with relative diversity study of avifauna of Jaipurhat district of Bangladesh",
"abstract": "<p>This investigation was carried out to observe the migration status and the feeding guild of the avian fauna including the relative diversity of Jaipurhat district of Bangladesh. Data were collected from November 2017 to October 2019. During the study period, 89 avian species were recorded that belong to 32 families under 11 orders among which 8 (8.99%) species were common winter visitors, 3 (3.37%) were common summer visitors, 8 (8.99%) were resident migratory, 1 (1.12%) were rare resident and 69 (77.53%) were resident birds. Among the birds observed in the study area during the study period the highest number of birds was found to be insectivorous (41.57%), then the carnivorous (8.99%), then omnivorous, piscivorous and picio carnivorous (7.87%), then insecto frugivorous (6.67%), then frugivorous (5.62%), then frugio graminivorous (4.49%), then nectarivorous (3.39%), then graminivorous and molluscio carnivorous (2.25%) and insecto carnivorous (1.12%). The hight avian diversity (69.662%) was observed in Mithapur which is human inhabited area with crop lands and a lot of fruit trees and the lowest (32.584%) diversity was in Shree Rampur Bill and Kastogari Bill which has large water bodies. This study will be helpful to understand the status of birds in different season in the study area.</p>",
"DOI": "10.5455/jabet.2020.d126",
"views": 960,
"downloads": 133,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/15/178-1582884845-Figure1.jpg",
"caption": "Figure 1. Migration status of birds in the study area.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/15/178-1582884845-Figure2.jpg",
"caption": "Figure 2. Social status of birds in the study area.(S = Single, P = Paired, SF = Small flock, LF = Large flock, S/P = Single or paired, SF/LF = Small or large flock, S/SF = Single or small flock, P/SF = Pared or small flock, S/P/SF = Single or paired or small flock, S/P/LF = Single or paired or large flock, S/SF/LF = Single or Small flock or large flock.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/15/178-1582884845-Figure3.jpg",
"caption": "Figure 3. Feeding guild of birds of in study area (OM= Omnivorous, CV = Carnivorous, IV = Insectivorous, FV = Frugivorous, PV = Piscivorous, GV = Graminivorous, NV = Nectarivorous, IV+FV = insecto frugivorous, FV + GV frugio graminivorous, PV + CV = picio carnivorous, IV + CV = insecto carnivorous, MV + CV = molluscio carnivorous).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/15/178-1582884845-Figure4.jpg",
"caption": "Figure 4. Relative diversity of birds.",
"featured": false
}
],
"authors": [
{
"id": 864,
"affiliation": [
{
"affiliation": "Institute of Biological Sciences, University of Rajshahi, Rajshahi, 6207, Bangladesh"
}
],
"first_name": "Al",
"family_name": "Amin",
"email": "alaminology1@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Al Amin, Institute of Biological Sciences, University of Rajshahi, Rajshahi, 6207, Bangladesh, E mail: alaminology1@gmail.com",
"article": 196
},
{
"id": 865,
"affiliation": [
{
"affiliation": "Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal"
}
],
"first_name": "Kamrul",
"family_name": "Hasan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 196
},
{
"id": 866,
"affiliation": [
{
"affiliation": "Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, 6207, Bangladesh"
}
],
"first_name": "Shafi",
"family_name": "Mahmud",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 196
},
{
"id": 867,
"affiliation": [
{
"affiliation": "Faculty of Fine Arts, University of Rajshahi, Rajshahi, 6207, Bangladesh"
}
],
"first_name": "Maria",
"family_name": "Akter",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 196
},
{
"id": 868,
"affiliation": [
{
"affiliation": "Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal"
}
],
"first_name": "Shabnam",
"family_name": "Sabiha",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 196
},
{
"id": 869,
"affiliation": [
{
"affiliation": "Rajshahi medical College, Dental unit, Rajshahi, Bangladesh"
}
],
"first_name": "Sumaiya",
"family_name": "Arabi",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 196
}
]
},
{
"id": 194,
"slug": "178-1585380993-antibacterial-and-cytotoxic-activities-of-four-selected-summer-season-fruits-seeds-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1585380993",
"published": "2020-06-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/33/178-1585380993.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 194-203.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research Center, Bangladesh.",
"cite_info": "Rahman SA, Akhter MS. Antibacterial and cytotoxic activities of four selected summer season fruits’ seeds of Bangladesh. J Adv Biotechnol Exp Ther. 2020; 3(3): 194-203.",
"title": "Antibacterial and cytotoxic activities of four selected summer season fruits’ seeds of Bangladesh",
"abstract": "<p>The seeds of fruits which are thrown away as waste can be used in medicinal and therapeutic purposes. The goal of this investigation was to find out antibacterial and cytotoxic potentialities of crude acetone and ethanol extracts of seeds of four common summer fruits, purchased from Khulna region, Bangladesh: <em>Litchi chinensis</em> (Lychee), <em>Phoenix dactylifera</em> (Date Palm), <em>Syzigum cumini</em> (Black Plum) and <em>Artocarpus heterophyllus</em> (Jackfruit). Extracts were screened for their antibacterial activity by agar well diffusion method, followed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination by colorimetric broth macro dilution method and plating method. The highest antibacterial activity was found from acetone extract of <em>S. cumini</em> against <em>Salmonella typhi</em> where zone of inhibition was 19.67±0.27 mm along with MIC 0.0585 mg/ml and MBC 0.2344 mg/ml. Acetone extract of <em>S. cumini</em> was found to have bactericidal effect on all microorganisms except bacteriostatic on <em>E. coli</em> and <em>S. paratyphi</em> and ethanol extract showed bactericidal effect on all the microorganisms tested. The cytotoxic activity was determined by brine shrimp lethality assay. Among all seed samples, <em>P. dactylifera</em> exerted relatively better cytotoxicity where LC<sub>50</sub> value was below 100 µg/ml. The rest of the samples showed relatively less toxicity as LC<sub>50</sub> value was more than 100 µg/ml. Among all the seed samples, <em>S. cumini</em> showed most potential antibacterial activity with less cytotoxicity. Overall, the findings of the study endorse antibacterial activity of examined seed extracts with moderate cytotoxic activity, suggesting the isolation of active compounds thorough bioassays.</p>",
"DOI": "10.5455/jabet.2020.d125",
"views": 683,
"downloads": 124,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/14/178-1585380993-Figure1.jpg",
"caption": "Figure 1. Comparison of zone of inhibition of acetone extracts against tested microorganisms.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/14/178-1585380993-Figure2.jpg",
"caption": "Figure 2. Comparison of zone of inhibition of ethanol extracts against tested microorganisms.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/14/178-1585380993-Figure3.jpg",
"caption": "Figure 3. Potential zone of inhibitions obtained from acetone and ethanol extracts of selected samples: a. S. cumini : S. typhi, b. L. chinensis: S. aureus, c. P. dactylifera: P. aeruginosa, d. A. heterophyllus: Micrococcus spp., e. S. cumini: S. aureus, f. L. Chinensis: E. coli, g. P. dactylifera: P. aeruginosa, h. A. heterophyllus: V. cholerae (Figure a-d: acetone extracts, Figure e-h: ethanol extracts).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/14/178-1585380993-Figure4.jpg",
"caption": "Figure 4. Log concentration vs percent shrimp mortality of acetone extracts of the tested samples .",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/14/178-1585380993-Figure5.jpg",
"caption": "Figure 5. Log concentration vs percent shrimp mortality of ethanol extracts of the tested samples.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/14/178-1585380993-Figure6.jpg",
"caption": "Figure 6. Comparison of LC50 values of acetone and ethanol extracts (µg/ml) of the samples.",
"featured": false
}
],
"authors": [
{
"id": 856,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh."
}
],
"first_name": "Sheikh Ashikur",
"family_name": "Rahman",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 194
},
{
"id": 857,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh."
}
],
"first_name": "Md. Shamim",
"family_name": "Akhter",
"email": "shamim11akhter@bge.ku.ac.bd",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Shamim Akhter, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh. E-mail: shamim11akhter@bge.ku.ac.bd",
"article": 194
}
]
},
{
"id": 193,
"slug": "178-1588348425-genetic-fingerprinting-for-the-protection-of-local-rice-oryza-sativa-l-cultivars-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1588348425",
"published": "2020-06-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/35/178-1588348425.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 182-193.",
"academic_editor": "Dr. Mohammad Nazrul Islam, Sher-e-Bangla Agricultural University, Bangladesh.",
"cite_info": "Rahman MS, Sohag MKH, et al. Genetic fingerprinting for the protection of local rice (Oryza sativa L.) cultivars of Bangladesh. J Adv Biotechnol Exp Ther. 2020; 3(3): 182-193.",
"title": "Genetic fingerprinting for the protection of local rice (Oryza sativa L.) cultivars of Bangladesh",
"abstract": "<p>Genetic fingerprinting of 110 rice cultivars of Bangladesh was completed with five polymorphic microsatellite DNA markers such as RM153, RM251, RM333, RM335 and RM475. The amplified DNA fragments are known as alleles from Polymerase Chain Reaction (PCR) reactions were separated on 2% agarose gel electrophoresis system, subsequently visualized by high performance ultraviolet transilluminator. In all, 99 distinctive alleles averaging 19.80 alleles/locus from the entire utilized microsatellite loci were counted. Several diversity indexes such as Polymorphism Information Content (PIC), heterozygosity, and cluster analysis were computed in this quantitative investigation. Superior genetic differentiation and inferior gene flow values among the cultivars were revealed from the recorded genetic diversity study of PIC, Effective allele, Shannon index (<em>I</em>), Hardy-Weinberg equilibrium (HWE), Nei’s gene diversity (<em>h</em>), along with genetic differentiation-F<sub>is</sub> and gene flow-N<sub>m</sub> analysis. A total, 5995 varietal pairs were achieved all the way through alternative combinations of 110 rice cultivars where their Nei’s genetic distance (<em>D</em>) was ranged from zero to 2.832. Nei’s genetic-base an Unweight Pair Group Method of Arithmetic Means (UPGMA) diagram was assembled which eventually separated all the cultivars from each other according to their genetic distance and similarity. Thus, the finding of this study will expose such strategies to distinct all the wild relatives, cultivars and commercial varieties of rice or any other crop species having various genetic levels to facilitate further improvement and protection in future.</p>",
"DOI": "10.5455/jabet.2020.d124",
"views": 414,
"downloads": 79,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/14/178-1588348425-Figure1.jpg",
"caption": "Figure 1. A) Representative gel pictures of amplified alleles from five SSR markers RM153, RM251, RM333, RM335 and RM475 by using several rice cultivars of Bangladesh (Lane M=100bp Ladder; Lanes 1 to 28 represents rice cultivars (1=Lal Amon, 2=Lau Jan, 3=Nara Aswina, 4=Buna Dhan, 5=Begun Bechi, 6=Bhasha Manik, 7=Kochu Dhola, 8=Sunga Wala, 9=Konek Chul, 10=Horinkhur Panati, 11=Ganjia, 12=Dudhsar, 13=Mathia, 14=Khirma Pat, 15=Mukut Sail, 16=Ban Kolom, 17=Safa Har (3), 18=Kal Nania, 19=Shil Pan, 20=Jabar Sail, 21=Moisha Mida, 22=Paglakushyari, 23=Pan Kaich, 24=Lal Patjat, 25=Chandda Gotok, 26=Moisha Mira, 27=Choia Mora, 28=Modhu Maloti) [38]. B) B. Representative gel pictures of amplified alleles from five SSR markers RM153, RM251, RM333, RM335 and RM475 by using several rice cultivars of Bangladesh (Lane M=100bp Ladder; Lanes 29 to 56 represents rice cultivars (29=Kali Gochya, 30=Ludi Gochya, 31=Kala Gura, 32=Monura, 33=Moisa Mira, 34=Chand Moni (3), 35=Kamoni Sail, 36=Juna, 37=Kurki, 38=Arai Raj, 39=Kala Gora, 40=Mulai, 41=Dharga Sail, 42=Bondyl, 43=Chakkol, 44=Rajamun, 45=Kanchon Mogi, 46=Hiruyal, 47=Chapa Mali, 48=Deppol, 49=Pushon, 50=Kali Cochr, 51=Ghori Amon, 52=Muirol, 53=Muar Sail, 54=Jhaw Lota, 55=Bhoban, 56=Ful Badam), C) Representative gel pictures of amplified alleles from five SSR markers RM153, RM251, RM333, RM335 and RM475 by using several rice cultivars of Bangladesh (Lane M=100bp Ladder; Lanes 57 to 84 represents rice cultivars (57=Galong, 58=Hasna Chikon, 59=Thakor, 60=Tembur, 61=Tulsi Mala, 62=Giring, 63=Nag Pechi, 64=Bhua Dhan, 65=Sandik Sail, 66=Jhoria Sail, 67=Halde Medi, 68=Maitya Cheng, 69=Kala Bail, 70=Murki Balam, 71=Gulchamlaish, 72=Raj Kumari, 73=Hati Banda, 74=Kui Sail (2), 75=Chakkol (Muta) Raozan, 76=Muijuri, 77=Munsi Sail, 78=Thakur Dhan, 79=Moina Sail, 80=Butu Balam(2), 81=Kali Jira (2), 82=Jhual Kata, 83=Raj Bhog, 84=Lal Modonga), and D) Representative gel pictures of amplified alleles from five SSR markers RM153, RM251, RM333, RM335 and RM475 by using several rice cultivars of Bangladesh (Lane M=100bp Ladder; Lanes 85 to 110 represents rice cultivars (85=Surma Sail, 86=Madhu Mala, 87=Dumai Sail, 88=Chadlash, 89=Jola Bhangh, 90=Mutonga (2), 91=Jula Gudi, 92=Gutok, 93=Pani Torong, 94=Randar, 95=Bhor Gelam, 96=Neel Huri, 97=Jol Kumari, 98=Ful Kari, 99=Biropa, 100=Asham Baba, 101=Dhul Abiz, 102=Porangi, 103=Tulo Sail, 104=Khoni Dhan, 105=Choro, 106=Sundar Sail, 107=Bokra, 108=Nagra, 109=ACI 1, 110=Aalok 932024).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/38/14/178-1588348425-Figure2.jpg",
"caption": "Figure 2. UPGMA phylogenetic tree based on Nei’s [63] genetic distance showing the genetic relationship among 110 rice cultivars (Group, A= Transplant Aman, B= Broadcast Aman, C= Boro and Jhum)",
"featured": false
}
],
"authors": [
{
"id": 850,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Faculty of Agriculture Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh."
}
],
"first_name": "Md Shafikur",
"family_name": "Rahman",
"email": "shafikjss@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Shafikur Rahman, E-mail: shafikjss@gmail.com, Phone: +8801717469112",
"article": 193
},
{
"id": 851,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Md Kamrul Hasan",
"family_name": "Sohag",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 193
},
{
"id": 852,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Lutfur",
"family_name": "Rahman",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 193
},
{
"id": 853,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Md Shah-E-",
"family_name": "Alam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 193
},
{
"id": 854,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Ujjal Kumar",
"family_name": "Nath",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 193
},
{
"id": 855,
"affiliation": [
{
"affiliation": "Genetic Resource & Seed Division, Bangladesh Rice Research Institute, Joydebpur, Gazipur, Bangladesh"
}
],
"first_name": "Md. Khairul",
"family_name": "Bashar",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 193
}
]
},
{
"id": 187,
"slug": "178-1585833379-culture-positivism-exploitation-through-automated-fluorescent-sensor-technology-from-patients-with-blood-stream-infections",
"type": "original_article",
"manuscript_id": "178-1585833379",
"published": "2020-06-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/29/178-1585833379.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 165-170.",
"academic_editor": "Dr. Hasan-Al-Faruque, Daegu Gyeonbuk Institute of Science and Technology, South Korea.",
"cite_info": "Abedin MZ, Jarin L, et al. Culture positivism exploitation through automated fluorescent-sensor technology from patients with blood stream infections. J Adv Biotechnol Exp Ther. 2020; 3(3): 165-170.",
"title": "Culture positivism exploitation through automated fluorescent-sensor technology from patients with blood stream infections",
"abstract": "<p>This study tracks and analyses the culture results of 3615 blood samples received in Popular Diagnostic Centre, Dhanmondi, Dhaka 1205, throughout twelve month from suspected patients with blood stream infections. The samples were prepared by exploitation machine-controlled Fluorescent-Sensor Technology by BACTEC 9120® Culture System (Becton Dickinson and Company, Sparks, USA) choosing a 5 days incubation protocol. A total of 668/3615(18.50%) BACTEC 9120® system positive samples were then sub-cultured in 7% sheep blood agar, MacConkey agar, and chocolate agar plates. A sum of 346/668 (51.8%) were infective organisms and 312/668 (46.70%) positive vial cultures were contaminants. False positivism rate was 1.5% (10/668). The mean detection time for the clinical vital isolates was 17.7 h and for all the isolates was 38.1 h. Microorganisms characterization and antibiotic sensitivity testing was done using typical ways. The majorities (342, 98.84%) of those were Gram-negative microorganisms and solely 4 (1.16%) isolates were Gram-positive pathogens. Clinically vital pathogens recouped on day one, two and three were 91.0%, 7.5% and 1.5% severally. Most of the bacterial isolates were found extremely susceptible to a number of antibiotics along with ceftriaxone (95%), ceftazidine (95%), and cefepime (96%), whereas moderately sensitive to ciprofloxacin (81%), levofloxacin (89%) and chloramphenicol (81%); whereas all bacterial isolates were found to be resistant of nalidic acid (100%). Since all our cultures were positive at intervals the primary 72 h, our data supported 5 days incubation system for the recuperation of medically vital microorganisms in BACTEC 9120® Culture System.</p>",
"DOI": "10.5455/jabet.2020.d122",
"views": 188,
"downloads": 136,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/17/178-1585833379-Figure1.jpg",
"caption": "Figure 1. Total range of clinically vital microorganisms and detection time.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/17/178-1585833379-Figure2.jpg",
"caption": "Figure 2. Anti-biogram sensitivity and resistant pattern of blood culture isolates (AML=Amoxycillin, AZM= Azithromycin, CRO=Ceftriaxone, CAZ=Ceftazidime, CIP=Ciprofloxacin, C=Chloramphenicol, FEP=Cefepime, LEV=Levofloxacin, and NA= Nalidic acid).",
"featured": false
}
],
"authors": [
{
"id": 812,
"affiliation": [
{
"affiliation": "Department of Microbiology, School of Biomedical Science, Khwaja Yunus Ali University, Bangladesh."
}
],
"first_name": "Mohammad Zakerin",
"family_name": "Abedin",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 187
},
{
"id": 813,
"affiliation": [
{
"affiliation": "Department of Microbiology, LabAid Medical Centre Gulshan Ltd, Bangladesh."
}
],
"first_name": "Laila",
"family_name": "Jarin",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 187
},
{
"id": 814,
"affiliation": [
{
"affiliation": "Global Biotechnology & Biomedical Research Network (GBBRN), Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh."
},
{
"affiliation": "Center for Neuroscience, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, 02792, South Korea."
}
],
"first_name": "Md. Ataur",
"family_name": "Rahman",
"email": "ataur1981rahman@hotmail.com",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Ataur Rahman, Email: ataur1981rahman@hotmail.com",
"article": 187
},
{
"id": 815,
"affiliation": [
{
"affiliation": "Global Biotechnology & Biomedical Research Network (GBBRN), Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh."
},
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh."
}
],
"first_name": "Rokibul",
"family_name": "Islam",
"email": "rakibbgeiu@yahoo.com",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Rokibul Islam, Email: rakibbgeiu@yahoo.com",
"article": 187
}
]
},
{
"id": 186,
"slug": "178-1586622646-anxiolytic-and-thrombolytic-investigation-of-methanol-extract-of-piper-nigrum-l-fruits-and-sesamum-indicum-l-seeds",
"type": "original_article",
"manuscript_id": "178-1586622646",
"published": "2020-06-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/44/178-1586622646.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 158-164.",
"academic_editor": "Dr. Md. Abdul Hannan, Dongguk University, South Korea.",
"cite_info": "Emon NU, Kaiser M, et al. Anxiolytic and thrombolytic investigation of methanol extract of Piper nigrum L. fruits and Sesamum indicum L. seeds. J Adv Biotechnol Exp Ther. 2020; 3(3): 158-164.",
"title": "Anxiolytic and thrombolytic investigation of methanol extract of Piper nigrum L. fruits and Sesamum indicum L. seeds",
"abstract": "<p>The study was intended to compare and evaluate the <em>in vitro</em> thrombolytic activity and <em>in vivo</em> anxiolytic activities of methanol extracts of <em>Piper nigrum</em> fruits (MEPNF) and <em>Sesamum indicum</em> seeds (MESIS). An <em>in vitro</em> thrombolytic activity was employed by investigating the clot lysis effect of MEPNF, MESIS, and their combinations. <em>In vivo</em> anxiolytic activity of MEPNF and MESIS was examined by using the Elevated Plus Maze (EPM) test considering diazepam (1 mg/kg) as the standard drug. In the thrombolytic test, MEPNF and MESIS showed a moderate (<em>P</em> < 0.01) clot lysis activity. Besides, MEPNF and MESIS showed significant (<em>P</em> < 0.001) dose-dependent anxiolytic activity. Since MEPNF or MESIS 200 and 400 (mg/kg, b.w; p.o) has been administered, the time spent in the open arms, and the number of entry into the open arms were increased. The finding suggests that MEPNF and MESIS may be the source for biological activity and therefore further research is required for confirmation.</p>",
"DOI": "10.5455/jabet.2020.d121",
"views": 659,
"downloads": 105,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/15/178-1586622646-Figure1.jpg",
"caption": "Figure 1. In vitro thrombolytic activity of test samples. Values are presented as mean ± SEM; One-way analysis of variance (ANOVA) followed by Dunnett’s test. *P <0.05, **P <0.01, and ***P < 0.001 is considered as significant compared with the control. Note: MEPNF = Methanol extract of Piper nigrum fruits and MESIS = Methanol extract Sesamum indicum seeds, SW = 1% Tween 80, and SKT = Streptokinase.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/15/178-1586622646-Figure2.jpg",
"caption": "Figure 2. In vitro thrombolytic activity (number of the entry in open arms) of test samples. Values are presented as mean ± SEM; One-way analysis of variance (ANOVA) followed by Dunnett’s test. **P <0.01, and ***P < 0.001 is considered as significant compared with the control. Note: MEPNF = Methanol extract of Piper nigrum fruits and MESIS = Methanol extract Sesamum indicum seeds, TWN = 1% Tween 80, and DFN = Diazepam.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/15/178-1586622646-Figure3.jpg",
"caption": "Figure 3. In vivo anxiolytic activity (time spent in open arms) of test samples. Values are presented as mean ± SEM; One-way analysis of variance (ANOVA) followed by Dunnett’s test. ***P < 0.001 is considered as significant compared with the control. Note: MEPNF = Methanol extract of Piper nigrum fruits and MESIS = Methanol extract Sesamum indicum seeds, TWN = 1% Tween 80, and DFN = Diazepam.",
"featured": false
}
],
"authors": [
{
"id": 802,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Nazim Uddin",
"family_name": "Emon",
"email": "nazim7emon@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-7567-4796",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Nazim Uddin Emon, Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh, Phone:+8801648094589, E-mail: nazim7emon@gmail.com",
"article": 186
},
{
"id": 803,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Md",
"family_name": "Kaiser",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 186
},
{
"id": 804,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohidol",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 186
},
{
"id": 805,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammed Faisal Ibna",
"family_name": "Kabir",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 186
},
{
"id": 806,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammad Jamir",
"family_name": "Uddin",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 186
},
{
"id": 807,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mifta Ahmed",
"family_name": "Jyoti",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 186
},
{
"id": 808,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Saifullah Mansur",
"family_name": "Tanjil",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 186
},
{
"id": 809,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Abu Nayem Mohammad",
"family_name": "Rasel",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 186
},
{
"id": 810,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh."
}
],
"first_name": "Safaet",
"family_name": "Alam",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 186
},
{
"id": 811,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammad Nazmul",
"family_name": "Islam",
"email": null,
"author_order": 10,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 186
}
]
},
{
"id": 178,
"slug": "178-1586843165-formaldehyde-contaminated-feed-induces-histopathological-changes-in-the-testes-of-adult-pigeons-columba-livia",
"type": "original_article",
"manuscript_id": "178-1586843165",
"published": "2020-06-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/27/178-1586843165.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 152-157.",
"academic_editor": "Dr. Md. Jamal Uddin, Ewha Womans University, South Korea.",
"cite_info": "Karim MR, Kobir A, et al. Formaldehyde-contaminated feed induces histopathological changes in the testes of adult pigeons (Columba livia). J Adv Biotechnol Exp Ther. 2020; 3(3): 152-157.",
"title": "Formaldehyde-contaminated feed induces histopathological changes in the testes of adult pigeons (Columba livia)",
"abstract": "<p>Formaldehyde (FA), a ubiquitous environmental pollutant, has long been suspected to possess reproductive toxicity. Here, we investigated the histopathological alteration of male gonads following exposure to FA-contaminated feed (40% aqueous solution of FA; 2.5 ml formalin/kg feed) in pigeons for 7 days. The mean body weights were not changed significantly in FA-contaminated feed exposed pigeons compared with control pigeons. The hemoglobin concentration was significantly decreased and serum enzyme aspartate transaminase (AST) was significantly increased in FA-exposed pigeons in comparison with control pigeons. Histologically, the structural components of the testes are the seminiferous tubules and interstitial tissues, which are surrounded by a connective tissue capsule. In control pigeons, the size and shape of seminiferous tubules were normal with a regular arrangement of spermatogenic cells. In FA-exposed pigeons, the testicular capsule was thickened and degeneration of spermatogenic cells in the seminiferous tubules was observed. The number of spermatogenic cells was significantly decreased in the seminiferous tubules of FA-exposed pigeons in comparison with control pigeons, indicating that the low exposure of FA affects the spermatogenic cells’ populations in male birds. The present results suggested that FA might cause infertility in birds.</p>",
"DOI": "10.5455/jabet.2020.d120",
"views": 632,
"downloads": 120,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/17/178-1586843165-Figure1.jpg",
"caption": "Figure 1. Body weight and blood parameters analysis of FA-exposed adult pigeons. A. No significant changes of body weight were seen in control and FA-exposed pigeons. B. Total erythrocyte count (TEC) showed no significant changes but showing decreasing tendency in FA-exposed pigeons in comparison with control. C. Hemoglobin content (Hb, g%) was significantly decreased in FA-exposed pigeons compared with control pigeons. D. Serum enzyme, aspartate aminotransferase (AST) level was significantly increased in FA-exposed pigeon compared with control. Student t-test, *The p-values of ≤0.05 were considered to be statistically significant, **p-values of <0.01 indicate highly significant results. FA- Formaldehyde.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/17/178-1586843165-Figure2.jpg",
"caption": "Figure 2. Gross anatomy of testes of adult pigeons. A. Showing normal color, size and shape of the right and left testes of control pigeons. B. Normal color and size, but uneven shape and pinpoint hemorrhage was seen in the both right and left testes of FA-exposed pigeons. FA- Formaldehyde. Bar = 1 cm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/17/178-1586843165-Figure3.jpg",
"caption": "Figure 3. Histology of capsule of testes of adult pigeons. A. Showing normal histology of capsule (consisting of dense irregular connective tissues) covering the testicular parenchyma. B. The thickness of the capsule was apparently high in the testis of FA-exposed pigeon. Star indicates the degeneration of spermatogenic cells. FA- Formaldehyde. Bar = 50 µm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/06/17/178-1586843165-Figure4.jpg",
"caption": "Figure 4. Histology of testes of adult pigeons. A. Showing many seminiferous tubules and interstitial tissues in testes of control pigeons. B. In higher magnification, a regular distribution of spermatogenic cells in seminiferous tubules of control pigeons. C-D. Degeneration of spermatogenic cells and large size lumen were seen in the seminiferous tubules of FA-exposed pigeons. In addition, the number of spermatogenic cells was drastically reduced in the seminiferous tubules of testis. E. The number of spermatogenic cells was significantly decreased in seminiferous tubules of FA-exposed pigeons in comparison with control pigeons. F-G. The interstitial cells or Leydig cells were seen between or among the seminiferous tubules and showing normal histology both in control and FA-exposed pigeons. Arrow indicates the interstitial cells or Leydig cells and arrowheads indicate sertoli cell. Stars indicate the degeneration areas of spermatogenic cells. SG-spermatogonia, SC-spermatocyte, ST-spermatid, SZ- spermatozoa. Student t-test, *p-values of <0.01 indicate significant results. FA- Formaldehyde. Bar = 50 µm.",
"featured": false
}
],
"authors": [
{
"id": 797,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Mohammad Rabiul",
"family_name": "Karim",
"email": "mrabiulkarim@bau.edu.bd",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Mohammad Rabiul Karim, Professor and Head, Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, E-mail: mrabiulkarim@bau.edu.bd",
"article": 178
},
{
"id": 798,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Alamgir",
"family_name": "Kobir",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 178
},
{
"id": 799,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Imam",
"family_name": "Hasan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 178
},
{
"id": 800,
"affiliation": [
{
"affiliation": "Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Munmun",
"family_name": "Pervin",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 178
},
{
"id": 801,
"affiliation": [
{
"affiliation": "Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt."
}
],
"first_name": "Ahmed I. Abo-",
"family_name": "Ahmed",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 178
}
]
},
{
"id": 190,
"slug": "178-1579228061-pharmacological-effect-of-methanolic-and-hydro-alcoholic-extract-of-coconut-endocarp",
"type": "original_article",
"manuscript_id": "178-1579228061",
"published": "2020-06-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/35/178-1579228061.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(3): 171-181.",
"academic_editor": "Dr. Md. Abdul Hannan, Dongguk University, South Korea.",
"cite_info": "Akter S, Shah M, et al. Pharmacological effect of methanolic and hydro-alcoholic extract of Coconut endocarp. J Adv Biotechnol Exp Ther. 2020; 3(3): 171-181.",
"title": "Pharmacological effect of methanolic and hydro-alcoholic extract of Coconut endocarp",
"abstract": "<p><em>Cocos nucifera </em>used as natural remedies in a wide variety of diseases. The current experiment aimed to determine the qualitative phytochemicals, anxiolytic, anti-diarrheal, anti-inflammatory, thrombolytic and cytotoxic actions of <em>C. nucifera</em> endocarp, which extracted by using methanol (MeOH-CNE) and the hydro-alcohol (HaE-CNE). The MeOH-CNE and HaE-CNE subjected to phytochemical screening, where both extracts showed the existence of secondary metabolites such as carbohydrates, flavonoids, cardiac glycosides, and proteins. The anxiolytic activity screened by elevated plus maze experiment, whereas the percentage of open arm accounts 74.55 ± 4.54%, 66.31 ± 4.41% at 400 mg/kg for HaE-CNE, MeOH-CNE respectively (<em>P < </em>0.05) while the standard drug Diazepam (75.24 ± 3.91%). In the anti-diarrheal test, extracts (200 and 400 mg/kg) and standard drug loperamide (5 mg/kg) showed dose-dependent significant (<em>P <</em> 0.05) inhibition against castor oil-induced diarrhea. The MeOH-CNE and HaE-CNE exhibited 72.91 ± 4.20 %, 64.42 ± 5.50% inhibition of protein denaturation at 500 μg/ml, while in the thrombolytic test, HaE-CNE showed the highest and significant (<em>P <</em> 0.05) in clot lysis activity on human blood in comparison to water, whereas streptokinase used as standard. In brine shrimp lethality bioassay, the LC<sub>50 </sub>of HaE-CNE and MeOH-CNE ware 432.35 µg/ml and 1173.88 µg/ml respectively. The LC<sub>50</sub> for standard vincristine sulfate was 43.15 µg/ml. The current results suggested that MeOH-CNE and HaE-CNE have promising pharmacological activity.</p>",
"DOI": "10.5455/jabet.2020.d123",
"views": 1047,
"downloads": 104,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/17/178-1579228061-Figure1.jpg",
"caption": "Figure 1. Effect of MeOH-CNE and HaE-CNE on percentage of entry into open arm in elevated plus maze test in mice. Values are mean ± S.E.M. *P < 0.05 and **P < 0.01, significantly different from control; ANOVA followed Dunnett’s test (n = 6, per group). Where, MeOH-CNE: methanolic extract Cocos nucifera endocarp and HaE-CNE: hydro-alcoholic extract of Cocos nucifera endocarp.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/17/178-1579228061-Figure2.jpg",
"caption": "Figure 2. Antidiarrheal activity of MeOH-CNE and HaE-CNE against castor oil-induced diarrhea in mice. All values are expressed as mean ± SEM (n = 6); Data were analyzed by one way analysis of variance using GraphPad Prism for Windows, Version 6.0 (GraphPad software Inc., San Diego, CA, USA) followed by Dunnett’s test for multiple comparisons,. Values with * P < 0.05 were considered as significant. MeOH-CNE: methanolic extract Cocos nucifera endocarp and HaE-CNE: hydro-alcoholic extract of Cocos nucifera endocarp.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/17/178-1579228061-Figure3.jpg",
"caption": "Figure 3. Percentage inhibition of protein denaturation by MeOH-CNE, HaE-CNE. Results are mean ± SEM (n = 3). ANOVA followed by Dunnett’s test. Where, MeOH-CNE: methanolic extract Cocos nucifera endocarp and HaE-CNE: hydro-alcoholic extract of Cocos nucifera endocarp.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/17/178-1579228061-Figure4.jpg",
"caption": "Figure 4. Clot lysis by streptokinase, MeOH-CNE, HaE-CNE and water in vitro. Values are expressed as mean ± S.E.M. P < 0.05, significantly different from control; ANOVA followed by Dunnett’s test. Where, MeOH-CNE: methanolic extract Cocos nucifera endocarp and HaE-CNE: hydro-alcoholic extract of Cocos nucifera endocarp.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/17/178-1579228061-Figure5.jpg",
"caption": "Figure 5. Cytotoxic effects of MeOH-CNE and HaE-CNE. Extrapolation of inhibition concentrations of both MeOH-CNE, HaE-CNE and reference cytotoxic agent vincristine sulfate through regression analysis. Data are shown as mean ± SEM of 10 shrimps for each concentration. MeOH-CNE: methanolic extract Cocos nucifera endocarp and HaE-CNE: hydro-alcoholic extract of Cocos nucifera endocarp.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/26/17/178-1579228061-Figure6.jpg",
"caption": "Figure 6. Graphical representations of pharmacological activities of Cocos nucifera endocarp.",
"featured": false
}
],
"authors": [
{
"id": 832,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Saimun",
"family_name": "Akter",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 190
},
{
"id": 833,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Masud",
"family_name": "Shah",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 190
},
{
"id": 834,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
}
],
"first_name": "Abu Montakim",
"family_name": "Tareq",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 190
},
{
"id": 835,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
},
{
"affiliation": "Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh"
}
],
"first_name": "Mst. Samima",
"family_name": "Nasrin",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 190
},
{
"id": 836,
"affiliation": [
{
"affiliation": "Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh"
}
],
"first_name": "Md. Atiar",
"family_name": "Rahman",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 190
},
{
"id": 837,
"affiliation": [
{
"affiliation": "International Institute for Halal Research and Training, KICT Building, Level 3, Jalan Gombak, 53100, Selangor, Malaysia"
}
],
"first_name": "Z. M.",
"family_name": "Babar",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 190
},
{
"id": 838,
"affiliation": [
{
"affiliation": "Department of Experimental Pathology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan"
}
],
"first_name": "Md Anwarul",
"family_name": "Haque",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 190
},
{
"id": 839,
"affiliation": [
{
"affiliation": "School of Life Science, University of Electronic Science and Technology of China, Sichuan, China"
}
],
"first_name": "Mohammad Jahir",
"family_name": "Royhan",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 190
},
{
"id": 840,
"affiliation": [
{
"affiliation": "Department of Applied Chemistry and Chemical Engineering, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh"
}
],
"first_name": "Miskatun Nur",
"family_name": "Mamun",
"email": null,
"author_order": 9,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 190
},
{
"id": 841,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh"
},
{
"affiliation": "Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh"
}
],
"first_name": "A. S. M. Ali",
"family_name": "Reza",
"email": "alirezaru@gmail.com",
"author_order": 10,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "A.S.M. Ali Reza, Tel: +88-031-610085, E-mail: alirezaru@gmail.com",
"article": 190
},
{
"id": 842,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh"
}
],
"first_name": "Talha Bin",
"family_name": "Emran",
"email": "talhabmb@gmail.com",
"author_order": 11,
"ORCID": "https://orcid.org/0000-0003-3188-2272",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran, Cell: +88-01819942214, E-mail: talhabmb@gmail.com or talhabmb@bgctub.ac.bd",
"article": 190
}
]
}
]
},
{
"id": 11,
"title": "Vol3 Issue2",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2020",
"slug": "2020-vol3-issue2",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 146,
"slug": "178-1581536838-possible-neuropharmacological-effects-of-apis-cerana-indica-beehive-in-the-swiss-albino-mice",
"type": "original_article",
"manuscript_id": "178-1581536838",
"published": "2020-05-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/33/178-1581536838.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(2): 128-134.",
"academic_editor": "Dr. Md. Abdul Hannan, Dongguk University, South Korea.",
"cite_info": "Tareq AM, Sohel M, et al. Possible neuropharmacological effects of Apis cerana indica beehive in the Swiss Albino mice. J Adv Biotechnol Exp Ther. 2020; 3(2): 128-134.",
"title": "Possible neuropharmacological effects of Apis cerana indica beehive in the Swiss Albino mice",
"abstract": "<p>The water-soluble extract is a gummy semi-solid content of <em>Apis cerana</em> <em>indica </em>beehive (WSE-BH). The present study reports the neuropharmacological effects of beehive derived from <em>Apis cerana</em> <em>indica</em>. The neuropharmacological results evaluated by modified open field, hole cross, elevated plus maze and hole board (OF-HC-EPM-HBT) test by Swiss Albino mice of both sexes after single oral administration where parameters for sedative and anxiolytic activity was square movements, hole crossing, time spent in open arm and head dipping which is the unpunished or unlearned response. A time-dependent manner activity observed by WSE-BH (200 and 400 mg/kg) and diazepam (1 mg/kg) against negative control normal saline. At low dose (200 mg/kg), the OF and HC possess significant reducing effects in time dependence manner while EPM and HBT exhibited significant anxiolytic activity avoiding sedation, whereas at 400 mg/kg exhibited an irregular effect. The current results were suggesting that WSE-BH might a good source of anxiolytic and sedative effects at low dose concentration.</p>",
"DOI": "10.5455/jabet.2020.d117",
"views": 463,
"downloads": 95,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/17/178-1581536838-Figure1.jpg",
"caption": "Figure 1. Effects of water soluble extract of beehive on open filed test by mice in different time intervals. The result characterized in mean ± SEM (n=5). a P < 0.05, b P < 0.01 and d P < 0.0001. The statistical analysis followed by unpaired t-test of GraphPad Prism (ver 7) in comparison with negative control (normal saline 0.9 % NaCl). WSE-BH - water soluble extract of beehive.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/17/178-1581536838-Figure2.jpg",
"caption": "Figure 2. Effects of water-soluble extract of beehive on hole cross test by mice in different time intervals. The result characterized in mean ± SEM (n=5). a P < 0.05, and c P < 0.001. The statistical analysis followed by unpaired t-test of GraphPad Prism (ver 7) in comparison with negative control (normal saline 0.9 % NaCl). WSE-BH - water soluble extract of beehive.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/17/178-1581536838-Figure3.jpg",
"caption": "Figure 3. Effects of water soluble extract of beehive on percentage of time spent in open arm on elevated plus maze test by mice in different time intervals. The result characterized in mean ± SEM (n=5). a P < 0.05, b P < 0.01, and d P < 0.0001. The statistical analysis followed by unpaired t-test of GraphPad Prism (ver 7) in comparison with negative control (normal saline 0.9 % NaCl). WSE-BH - water soluble extract of beehive.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/17/178-1581536838-Figure4.jpg",
"caption": "Figure 4. Effects of water soluble extract of beehive on number of head dipping on hole board test by mice in different time intervals. The result characterized in mean ± SEM (n=5). a P < 0.05, b P < 0.01, and d P < 0.0001. The statistical analysis followed by unpaired t-test of GraphPad Prism (ver 7) in comparison with negative control (normal saline 0.9 % NaCl). WSE-BH - water soluble extract of beehive.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/17/178-1581536838-Figure5.jpg",
"caption": "Figure 5. Graphical representation of neuropharmacological effects of water-soluble extract (WSE) of beehive.",
"featured": false
}
],
"authors": [
{
"id": 611,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Abu Montakim",
"family_name": "Tareq",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 146
},
{
"id": 612,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammad",
"family_name": "Sohel",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 146
},
{
"id": 613,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Main",
"family_name": "Uddin",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 146
},
{
"id": 614,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Md. Hasan",
"family_name": "Mahmud",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 146
},
{
"id": 615,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
},
{
"affiliation": "Drug Discovery, GUSTO A Research Group, Chittagong-4000, Bangladesh"
}
],
"first_name": "Muminul",
"family_name": "Hoque",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 146
},
{
"id": 616,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "A.S.M. Ali",
"family_name": "Reza",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 146
},
{
"id": 617,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mst. Samima",
"family_name": "Nasrin",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 146
},
{
"id": 618,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Faisal Bin",
"family_name": "Kader",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 146
},
{
"id": 619,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
},
{
"affiliation": "Drug Discovery, GUSTO A Research Group, Chittagong-4000, Bangladesh"
}
],
"first_name": "Faisal Bin",
"family_name": "Kader",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 146
},
{
"id": 620,
"affiliation": [
{
"affiliation": "Drug Discovery, GUSTO A Research Group, Chittagong-4000, Bangladesh"
},
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chittagong-4381, Bangladesh"
}
],
"first_name": "Talha Bin",
"family_name": "Emran",
"email": "talhabmb@gmail.com",
"author_order": 9,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran PhD, Assistant Professor, Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh. Cell: +88-01819942214, E-mail: talhabmb@gmail.com or talhabmb@bgctub.ac.bd",
"article": 146
}
]
},
{
"id": 176,
"slug": "178-1584771498-developing-pseudomonas-aeruginosa-mutants-with-hyper-proteolytic-activity-through-uv-mutagenesis-and-characterization-for-optimized-production",
"type": "original_article",
"manuscript_id": "178-1584771498",
"published": "2020-05-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/17/178-1584771498.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(2): 135-142.",
"academic_editor": "Dr. Md Nabiul Islam, Yamaguchi University School of Medicine, Japan.",
"cite_info": "Rakib K, Nain Z, et al. Developing Pseudomonas aeruginosa mutants with hyper-proteolytic activity through UV mutagenesis and characterization for optimized production. J Adv Biotechnol Exp Ther. 2020; 3(2): 135-142.",
"title": "Developing Pseudomonas aeruginosa mutants with hyper-proteolytic activity through UV mutagenesis and characterization for optimized production",
"abstract": "<p>Over 60% of the global productions of industrial enzymes are proteolytic enzymes in which about 35% are alkaline proteases. The current microbial sources are unable to reach industrial demands of alkaline protease which led scientists to search new sources with enhanced enzyme activity. Therefore, we applied UV irradiation to develop a <em>Pseudomonas</em> <em>aeruginosa</em> mutant as a new source of protease overproduction, followed by cultural and nutritional optimizations. The mutagenesis was carried out by exposing parent strains to UV radiation (30w, 2537 Å) at 25 ºC with a different time interval. The protease activity was estimated as relative protease activity and standard protease assay (OD<sub>660</sub>). Among all, mutant strain <em>P. aeruginosa</em>–M25 (PA-M25) exhibited 75.47% increased protease activity over the parent strain in submerged fermentation. It showed 612.84±2.50 U/ml of alkaline protease production compared to 349.26±2.57 U/ml by wild-type strain (significant at <em>P</em>≤0.005). Besides, the effects of nutritional factors on the protease production by PA-M25 were also studied. We found the optimal alkaline protease production in the medium (adjusted to pH 9.0) supplemented with 1% (w/v) glucose as carbon source, 0.5% (w/v) casein as nitrogen source and 2% (w/v) NaCl when incubated at 35 ºC for 48 h without agitation. We believe that the mutant PA-M25 could be a potential candidate to meet the growing protease demands. However, further assessments regarding the characterization of the protease enzyme, as well as the industrial fitness of the mutant, are warranted.</p>",
"DOI": "10.5455/jabet.2020.d118",
"views": 361,
"downloads": 87,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/40/17/178-1584771498-Figure1.jpg",
"caption": "Figure 1. Effect of physical parameters on alkaline protease production by the wild and mutant strain of P. aeruginosa MZ2F: (a) incubation temperature, (b) pH of medium, and (c) incubation period. The number of asterisk sign indicates the level of significant protease activity as p≤0.05 (*), p≤0.01 (**), and p≤0.005 (***) while ‘ns’ denotes non-significant.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/40/17/178-1584771498-Figure2.jpg",
"caption": "Figure 2. Effect of medium ingredients on alkaline protease production by the wild and mutant strain of P. aeruginosa MZ2F: (a) carbon sources, (b) nitrogen sources, and (c) salt concentration. The number of asterisk sign indicates the level of significant protease activity as p≤0.05 (*), p≤0.01 (**), and p≤0.005 (***) while ‘ns’ denotes non-significant.",
"featured": false
}
],
"authors": [
{
"id": 750,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh."
}
],
"first_name": "Kariul",
"family_name": "Rakib",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 176
},
{
"id": 751,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh."
},
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Faculty of Sciences and Engineering, East West University, Dhaka-1212, Bangladesh."
}
],
"first_name": "Zulkar",
"family_name": "Nain",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 176
},
{
"id": 752,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh."
}
],
"first_name": "Bulbul",
"family_name": "Ahammed",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 176
},
{
"id": 753,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh."
}
],
"first_name": "Mohammad Minnatul",
"family_name": "Karim",
"email": "mkmicro.du@gmail.com",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Minnatul Karim, Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh; Email: mkmicro.du@gmail.com; Tel.: +880 1620 191993",
"article": 176
}
]
},
{
"id": 177,
"slug": "178-1585668883-identifying-and-locating-superior-gluteal-artery-perforator-on-vietnamese-adults-a-study-for-clinical-applications",
"type": "original_article",
"manuscript_id": "178-1585668883",
"published": "2020-05-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/50/178-1585668883.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(2): 143-151.",
"academic_editor": "Dr. Md. Niamul Haque,Korea University, South Korea.",
"cite_info": "Khoa TD, Son TT, et al. Identifying and locating superior gluteal artery perforator on Vietnamese adults – A study for clinical applications. J Adv Biotechnol Exp Ther. 2020; 3(2): 143-151.",
"title": "Identifying and locating superior gluteal artery perforator on Vietnamese adults – A study for clinical applications",
"abstract": "<p>The superior gluteal artery (SGA) perforator flap (SGAP) used to cover lumbosacral soft tissue defects, in order to take advantage of the SGAP flap, all surgeons are required to have substantial knowledge of its cutaneous distribution of perforator. This study describes the anatomical characteristics of the perforators of SGA on Vietnamese adults; and locates the perforator of SGA on the gluteal region. We carried out on 32 Vietnamese adult cadavers at Department of Anatomy of Ho Chi Minh University of Medicine and Pharmacy, from Jan 2017 to June 2019. On average, SGA gives off 4-5 perforators, while none was found on the deep branch. The average length of perforator from the skin to the dissecting point is short compared to that of from skin to origin diameter at origin is quite large (1mm). The chance of finding 3-5 perforators in the upper triangle is 75% and 87% on the right and left side, respectively. Also, the probability of finding a single perforator in the upper triangle is 100% based on the x-axis, most perforators are found in segment 2/5 to segment 3/5 with the center being the midpoint between two segments; based on the y-axis, most perforators are found in segment 1.5/5 to segment 3.5/5 with the center being the midpoint between the two.</p>",
"DOI": "10.5455/jabet.2020.d119",
"views": 587,
"downloads": 113,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/17/178-1585668883-Figure1.jpg",
"caption": "Figure 1. Landmarks and gluteal coordinate system A. Landmarks and gluteal coordinate system; B. Measure the coordinates where the SGA sends the perforators to enter the overlying skin. (Source: specimen R.476).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/17/178-1585668883-Figure2.jpg",
"caption": "Figure 2. Muscular and septal perforator. A. Course of muscular perforator from the superficial branch of the right SGA; B. Interseptal course of septal perforator from the superficial branch of SGA between the gluteus maximus and medius muscles (Source: 2A- specimen T.566, 2B- specimen H. 546).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/17/178-1585668883-Figure3.jpg",
"caption": "Figure 3. Locating perforators entering the skin. A. Upper triangle has 3 perforators, B. Identifying perforators based on 5 equal segments (Source: 3A- specimen T. 589; 3B- specimen R. 476).",
"featured": false
}
],
"authors": [
{
"id": 754,
"affiliation": [
{
"affiliation": "Department of Anatomy, Pham Ngoc Thach University of Medicine (PNTU), Ho Chi Minh City, Vietnam"
}
],
"first_name": "Tran Dang",
"family_name": "Khoa",
"email": "khoatrandr@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Tran Dang Khoa, Department of Anatomy, Pham Ngoc Thach University of Medicine (PNTU), Ho Chi Minh City, Vietnam, E-mail: khoatrandr@gmail.com",
"article": 177
},
{
"id": 755,
"affiliation": [
{
"affiliation": "Department of Reconstructive and Plastic Surgery, Hanoi University of Medicine, Hanoi, Vietnam"
}
],
"first_name": "Tran Thiet",
"family_name": "Son",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 177
},
{
"id": 756,
"affiliation": [
{
"affiliation": "Department of Burn of Cho Ray Hospital, Ho Chi Minh City, Vietnam"
}
],
"first_name": "Le Nguyen Dien",
"family_name": "Minh",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 177
},
{
"id": 757,
"affiliation": [
{
"affiliation": "Thanh Van Plastic Hospital, Ho Chi Minh City, Vietnam"
}
],
"first_name": "Phan Tran Thanh",
"family_name": "Thao",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 177
}
]
},
{
"id": 144,
"slug": "178-1582512014-biodiversity-and-composition-of-the-herpetofauna-from-the-tien-hai-wetland-nature-reserve-north-vietnam",
"type": "original_article",
"manuscript_id": "178-1582512014",
"published": "2020-05-03",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/13/178-1582512014.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(2): 116-121.",
"academic_editor": "Dr. Shahed Uddin Ahmed Shazib, University of Ulsan, South Korea.",
"cite_info": "Le DT, Lo NT, et al. Biodiversity and composition of the herpetofauna from the Tien Hai Wetland Nature Reserve, North Vietnam. J Adv Biotechnol Exp Ther. 2020; 3(2): 116-121.",
"title": "Biodiversity and composition of the herpetofauna from the Tien Hai Wetland Nature Reserve, North Vietnam",
"abstract": "<p>Based on the novel data collected during the field surveys in 2019, we here in provided a checklist of eight species of amphibians belonging to seven genera (five families, one order) and nine species of reptiles belonging to eight genera (seven families, two orders) from Tien Hai Wetland Nature Reserve in Thai Binh Province. The species <em>Hemidactylus stejnegeri</em> is reported for the first time from Thai Binh Province. In terms of distribution pattern most of recorded species were found in the canal and aquaculture pond habitat (nine species of amphibians and reptiles, 52.94% of the total number of species). The diversity of amphibian and reptile species recorded from Tien Hai Wetland Nature Reserve is lower than the other wetland areas in North Vietnam. In this nature reserve, the number of species change according to salinity of amphibians is higher than of reptiles.</p>",
"DOI": "10.5455/jabet.2020.d115",
"views": 674,
"downloads": 156,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/17/178-1582512014-Figure1.jpg",
"caption": "Figure 1. Map showing the geographical location of Tien Hai Wetland Nature Reserve in the North Vietnam.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/17/178-1582512014-Figure2.jpg",
"caption": "Figure 2. The picture depicts a detailed view of the Tien Hai Wetland Nature Reserve with numbered collection localities.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/17/178-1582512014-Figure3.jpg",
"caption": "Figure 3. (A) Duttaphrynus melanostictus, (B) Microhyla fissipes, (C) Ferervarya limnocharis, (D) Hoplobatrachus rugolosus, (E) Sylvirana guentheri, and (F) Polypedates mutus.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/17/178-1582512014-Figure4.jpg",
"caption": "Figure 4. (A) Hemidactylus frenatus, (B) Hemidactylus stejnegeri, (C) Hypsiscopus plumbea, (D) Fowlea flavipunctatus, and (E) Lepidochelys olivacea.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/17/178-1582512014-Figure5.jpg",
"caption": "Figure 5. Habitats from Tien Hai Wetland Nature Reserve: A) Mangrove forest; B) Canal and aquaculture pond; C) Casuarina forest.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/17/178-1582512014-Figure6.jpg",
"caption": "Figure 6. Species diversity of amphibians and reptiles associated with habitat types in Tien Hai Wetland Nature Reserve, Thai Binh Province.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/17/178-1582512014-Figure7.jpg",
"caption": "Figure 7. Relationship between number of amphibia, reptile species and survey areas in Tien Hai Wetland Nature Reserve, Thai Binh Province.",
"featured": false
}
],
"authors": [
{
"id": 600,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, Hanoi, Vietnam"
}
],
"first_name": "Dzung Trung",
"family_name": "Le",
"email": "letrungdung_sp@hnue.edu.vn",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-5925-7797",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Le Trung Dung, Faculty of Biology, Hanoi National University of Education, Hanoi, Vietnam, E-mail: letrungdung_sp@hnue.edu.vn",
"article": 144
},
{
"id": 601,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, Hanoi, Vietnam"
}
],
"first_name": "Ngam Thi",
"family_name": "Lo",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 144
},
{
"id": 602,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, Hanoi, Vietnam"
}
],
"first_name": "Hai Nam",
"family_name": "Tran",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 144
},
{
"id": 603,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, Hanoi, Vietnam"
}
],
"first_name": "Yen Thi",
"family_name": "Do",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 144
}
]
},
{
"id": 145,
"slug": "178-1580307084-evidence-of-health-complications-caused-by-mosquito-coil-smoke-inhalation-in-mouse-model",
"type": "original_article",
"manuscript_id": "178-1580307084",
"published": "2020-05-03",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/54/178-1580307084.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(2): 122-127.",
"academic_editor": "Dr. Md Nabiul Islam, Yamaguchi University School of Medicine, Japan.",
"cite_info": "Karim MR, Ghose DK, et al. Evidence of health complications caused by mosquito coil smoke inhalation in mouse model. J Adv Biotechnol Exp Ther. 2020; 3(2): 122-127.",
"title": "Evidence of health complications caused by mosquito coil smoke inhalation in mouse model",
"abstract": "<p>Direct exposure to mosquito coil smoke (MCS) is a regular event in the entire life of most tropical country peoples, although it has countable significant health implications. However, noxious effect of mosquito coil smoke exposure on living being remains elusive. Herein, to address the mosquito coil smoke implication on health, Swiss albino mice were directly used to detect the health indicator alteration upon MCS administration as experimental model. In total 45 albino mice were grouped and exposed with MCS in two different time period viz, short time for 20 days and long time for 40 days. At the end of exposed time period, blood samples were collected and various health indicators analyzed. We revealed that biochemical indicators like serum aspartate aminotransferase (AST), alkaline phosphatase (ALP), alanine aminotransferase (ALT), bilirubin, urea, glucose, cholesterol, and triacylglycerol (TG) as well as hematological indicators like red blood cell (RBC), hemoglobin (Hb) and leukocyte (white blood cell) were significantly augmented in the exposed mice as compared to controls. In contrast, serum levels of total protein and albumin were remarkably decreased while serum creatinine and globulin level remained relatively unchanged. Furthermore, the microscopic analysis showed that the histopathological lesions were present on the lung tissue of exposed mice resulting, the alveolar septa and bronchiolar epithelial thickening, emphysema, and atelectasis of lung tissue as well as increased alveolar macrophages. Despite of, liver histopathology of the exposed mice showed same findings with a little deviation. Collectively, our findings propose that inhaling mosquito coil smoke induce significant health hazards on mice model followed by modification of vital health indicators and histological tissue-alignment alterations of lung and liver tissues.</p>",
"DOI": "10.5455/jabet.2020.d116",
"views": 1957,
"downloads": 215,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/52/17/178-1580307084-Figure1.jpg",
"caption": "Figure 1. Microscopic views of lung tissue of experimental mice. (a) Lung tissue slide was prepared and visualized by microscope using H&E staining from Group-I (Control) mice. (b) and (c) Lung tissue slide of mosquito coil smoke exposed mice (Group-II for 20 days and Group-III for 40 days) were visualized by microscope using H&E staining. (Microscopic view, X400).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/52/17/178-1580307084-Figure2.jpg",
"caption": "Figure 2. Microscopic views of liver tissue of experimental mice. (a) Liver tissue slide was prepared and visualized by microscope using H&E staining from Group-I (Control) mice. (b) and (c) Liver tissue slide of mosquito coil smoke exposed mice (Group-II for 20 days and Group-III for 40 days) were visualized by microscope using H & E staining. (Microscopic view, X400).",
"featured": false
}
],
"authors": [
{
"id": 604,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh."
},
{
"affiliation": "Department of Biochemistry and Biotechnology, Khwaja Yunus Ali University, Enayetpur, Sirajganj, Bangladesh."
}
],
"first_name": "Md. Rezaul",
"family_name": "Karim",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 145
},
{
"id": 605,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh."
}
],
"first_name": "Dipayon Krisna",
"family_name": "Ghose",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 145
},
{
"id": 606,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, Khwaja Yunus Ali University, Enayetpur, Sirajganj, Bangladesh."
}
],
"first_name": "Md Foyzur",
"family_name": "Rahman",
"email": "rahman.foyzur5239@gmail.com",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Foyzur Rahman, E-mail: rahman.foyzur5239@gmail.com",
"article": 145
},
{
"id": 607,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh."
}
],
"first_name": "Md. Tofazzal",
"family_name": "Hossain",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 145
},
{
"id": 608,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, Khwaja Yunus Ali University, Enayetpur, Sirajganj, Bangladesh."
}
],
"first_name": "Md. Rezanur",
"family_name": "Rahman",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 145
},
{
"id": 609,
"affiliation": [
{
"affiliation": "Global Biotechnology & Biomedical Research Network (GBBRN), Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh."
}
],
"first_name": "Md. Ataur",
"family_name": "Rahman",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 145
},
{
"id": 610,
"affiliation": [
{
"affiliation": "Global Biotechnology & Biomedical Research Network (GBBRN), Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh."
},
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh."
}
],
"first_name": "Rokibul",
"family_name": "Islam",
"email": "rakibbgeiu@yahoo.com",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Rokibul Islam, E-mail: rakibbgeiu@yahoo.com",
"article": 145
}
]
},
{
"id": 128,
"slug": "178-1579125981-phytochemical-screening-and-in-vitro-pharmacological-activities-of-methanolic-leaves-extract-of-caryota-mitis",
"type": "original_article",
"manuscript_id": "178-1579125981",
"published": "2020-05-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/00/178-1579125981.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(2): 109-115.",
"academic_editor": "Dr. Md. Abdul Hannan, Dongguk University, South Korea.",
"cite_info": "Tona MR, Tareq AM, et al. Phytochemical screening and in vitro pharmacological activities of methanolic leaves extract of Caryota mitis. J Adv Biotechnol Exp Ther. 2020; 3(2): 109 115.",
"title": "Phytochemical screening and in vitro pharmacological activities of methanolic leaves extract of Caryota mitis",
"abstract": "<p>The present study design to assess the qualitative phytochemical constituents responsible for anti-inflammatory, thrombolytic and cytotoxic activity of methanol extract of <em>Caryota mitis </em>leaves. The freshly collected<em> Caryota mitis </em>leaves extracted with methanol (MECM) to evaluate the secondary plant metabolites along with anti-inflammatory activity by protein denaturation assay. Thrombolytic and cytotoxic activity investigated using human blood clot lysis and brine shrimp lethality bioassay, respectively. The MECM exhibited several secondary plant metabolites named alkaloid, glycoside, protein, flavonoid, reducing sugar, saponins, and phenolic compound. The MECM extract (62.5-1000 μg/mL) exhibited an extremely significant (P < 0.0001) 6.06-45.45 % inhibition of protein denaturation against positive control diclofenac sodium (43.11-85.48%). In thrombolytic, the MECM exhibited 24.29 % significant (P < 0.0001) protection against blood clotting, whereas the positive control streptokinase (75.35%). In the cytotoxic study, a significant (P < 0.05) percentage of mortality exhibited while 70% mortality of Brine shrimp after 24 h at a concentration of 1000 μg/mL with an LC<sub>50</sub> value of 550.57 μg/mL whereas the positive control vincristine sulfate (1.63 μg/mL). The current results suggesting MECM has promising anti-inflammatory, thrombolytic activities with weak cytotoxicity.</p>",
"DOI": "10.5455/jabet.2020.d114",
"views": 882,
"downloads": 144,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/10/21/178-1579125981-Figure1.jpg",
"caption": "Figure 1. Percentage of inhibition of protein denaturation of anti-inflammatory activity by C. mitis leaves at different concentration. Results were represented as the mean ± SEM. (n = 3). * P < 0.0001 was statistically significant in comparison to Diclofenac Na followed by t-test of one-way ANOVA (GraphPad Prism 6). MECM= Methanolic extract of C. mitis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/10/21/178-1579125981-Figure2.jpg",
"caption": "Figure 2. Percentage of clot lysis of human blood by C. mitis leaves and positive control streptokinase. Results were represented as the mean ± SEM. (n = 5). * P < 0.0001 was statistically significant in comparison to negative control water followed by t-test of one-way ANOVA (GraphPad Prism 6). MECM= Methanolic extract of C. mitis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/10/21/178-1579125981-Figure3.jpg",
"caption": "Figure 3. Percentage of mortality of brine shrimp of C. mitis leaves and positive control at different concentrations. MECM= Methanolic extract of C. mitis, VCS= Vincristine sulfate.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/10/21/178-1579125981-Figure4.jpg",
"caption": "Figure 4. Graphical representation of pharmacological activities of methanolic leaves extract of C. mitis through in vitro approach.",
"featured": false
}
],
"authors": [
{
"id": 509,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Marzia Rahman",
"family_name": "Tona",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 128
},
{
"id": 510,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Abu Montakim",
"family_name": "Tareq",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 128
},
{
"id": 521,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammed Aktar",
"family_name": "Sayeed",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 128
},
{
"id": 522,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Md. Hasan",
"family_name": "Mahmud",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 128
},
{
"id": 523,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Israt",
"family_name": "Jahan",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 128
},
{
"id": 524,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Shahenur Alam",
"family_name": "Sakib",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 128
},
{
"id": 525,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chittagong-4381, Bangladesh"
}
],
"first_name": "Mutakabrun",
"family_name": "Shima",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 128
},
{
"id": 526,
"affiliation": [
{
"affiliation": "Drug Discovery, GUSTO A Research Group, Chittagong-4000, Bangladesh"
},
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chittagong-4381, Bangladesh"
}
],
"first_name": "Talha Bin",
"family_name": "Emran",
"email": "talhabmb@gmail.com",
"author_order": 8,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran PhD, Assistant Professor, Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh. Cell: +88-01819942214, E-mail: talhabmb@gmail.com or talhabmb@bgctub.ac.bd",
"article": 128
}
]
},
{
"id": 124,
"slug": "178-1575350410-a-general-introduction-of-propagation-and-micropropagation-techniques-of-macadamia-nut-macadamia-spp",
"type": "review_article",
"manuscript_id": "178-1575350410",
"published": "2020-05-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/29/178-1575350410.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(2): 102-108.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research Center, East Azampur, Dhaka, Bangladesh.",
"cite_info": "Rahman MS. A general introduction of propagation and micropropagation techniques of Macadamia Nut (Macadamia spp.). J Adv Biotechnol Exp Ther. 2020; 3(2): 102-108.",
"title": "A general introduction of propagation and micropropagation techniques of Macadamia Nut (Macadamia spp.)",
"abstract": "<p>Macadamia is categorized into a group of minor crop but can exhibit wide climatic adaptability. It has quick expanding industry demands for its high economic and nutritional values. Graftings, cuttings and micropropagations are the three major propagation methods of macadamia that can be utilized in a combination manner as seedlings with successful graftage can be encouraged to elongate and provide more scion woods for further multiplication such as supporting materials for cuttings. Cuttings can be sprouted to provide explant materials for micropropagation. Therefore, micropropagations are being used for commercial production of macadamia. Micropropagations are comparatively easy and convenient methods that can faster the introduction of any novel varieties into a new region, production of biotic stress tolerant plants, production of plants with greater uniformity and superior branching. Hence, this crop plant might hold an example for the other plants in the next few decades regarding much better application of advance propagation technologies and other genomic approaches.</p>",
"DOI": "10.5455/jabet.2020.d113",
"views": 1720,
"downloads": 186,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/21/178-1575350410-Figure1.jpg",
"caption": "Figure 1. Schematic diagram of different propagation techniques of macadamia nut.",
"featured": false
}
],
"authors": [
{
"id": 499,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Faculty of Agriculture, Patuakhali Science and Technology University, Patuakhali -8602, Bangladesh."
}
],
"first_name": "Md Shafikur",
"family_name": "Rahman",
"email": "shafikjss@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Shafikur Rahman, Department of Biotechnology, Faculty of Agriculture, Patuakhali Science and Technology University, Patuakhali -8602, Bangladesh. Tel: +8801717469112, Email: shafikjss@gmail.com",
"article": 124
}
]
},
{
"id": 123,
"slug": "178-1577511776-towards-the-antimicrobial-therapeutic-and-invasive-properties-of-mikania-micrantha-knuth-a-brief-overview",
"type": "review_article",
"manuscript_id": "178-1577511776",
"published": "2020-05-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/36/178-1577511776.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(2): 92-101.",
"academic_editor": "Dr. Hasan-Al-Faruque, Daegu Gyeonbuk Institute of Science and Technology, South Korea.",
"cite_info": "Sheam MM, Haque Z, et al. Towards the antimicrobial, therapeutic and invasive properties of Mikania micrantha Knuth: a brief overview. J Adv Biotechnol Exp Ther. 2020; 3(2): 92-101.",
"title": "Towards the antimicrobial, therapeutic and invasive properties of Mikania micrantha Knuth: a brief overview",
"abstract": "<p>Plant-derivatives impose a huge momentum in the field of medical science lately due to their wide-spectrum therapeutic attributes. Owing to the emerging drug resistance and hazardous side-effect of synthetic drugs, phytochemicals are now coming into play as a source of new and effective therapeutics. <em>Mikania micrantha</em> is a medicinal plant commonly found in tropical Asian countries including Bangladesh. The pharmacological significances of this plant were reported earlier which include a diverse range of antimicrobial and therapeutic potencies. However, the rapid-growing nature and covering surrounding flora reckoned <em>M. micrantha</em> as one of the world’s most invasive weeds. Therefore, it is essential to understand if the therapeutic essence of <em>M. micrantha</em> outweighs its invasiveness. In this brief review, we tried to explore the biological activities of <em>M. micrantha</em>. The future perspectives regarding the management of its invasiveness were also highlighted in this limited scope.</p>",
"DOI": "10.5455/jabet.2020.d112",
"views": 2983,
"downloads": 456,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/31/21/178-1577511776-Figure1.jpg",
"caption": "Figure 1. Pharmacological significances of Mikania micrantha plant. The central M. micrantha photograph was collected from National Inventory of Natural Heritage database (Captured by Cesar Delnatte).",
"featured": false
}
],
"authors": [
{
"id": 496,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Md Moinuddin",
"family_name": "Sheam",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 123
},
{
"id": 497,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Zahurul",
"family_name": "Haque",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 123
},
{
"id": 498,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh"
}
],
"first_name": "Zulkar",
"family_name": "Nain",
"email": "znain.bd@gmail.com",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Zulkar Nain, Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia-7003, Bangladesh; Email: znain.bd@gmail.com; Tel.: +880 1710 849539",
"article": 123
}
]
},
{
"id": 121,
"slug": "178-1577241188-the-medicinal-values-of-abrus-precatorius-a-review-study",
"type": "review_article",
"manuscript_id": "178-1577241188",
"published": "2020-05-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/28/178-1577241188.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(2): 84-91.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research Center, East Azampur, Dhaka, Bangladesh.",
"cite_info": "Bhakta S, Das SK. The medicinal values of Abrus precatorius: a review study. J Adv Biotechnol Exp Ther. 2020; 3(2): 84-91.",
"title": "The medicinal values of Abrus precatorius: a review study",
"abstract": "<p> Herbal medicines are in great demand for preliminary health care due to their wide medicinal values without any side effects. Since many species are used in the system of traditional medicine, scientists have great opportunities to develop appropriate packages of practices for their multiplication and conservation. It is reported that the plant has a broad range of therapeutic potentials, like anti-bacterial, anti-fungal, anti-tumor, analgesic, anti-spasmodic, anti-diabetic, anti-serotonergic, anti-migraine, including treatment of inflammation, ulcers, wounds, throat scratches and sores. <em>Abrus precatorius </em>is the native plant of this Indian subcontinent and used in many ways in the traditional system of medicine considered as Ayurveda treatment. Ayurveda recommends the administration of the <em>Abrus precatorius</em> in disease like alopecia, edema, helminths, skin diseases, itching, urinary disorders, and anti-fertility. This review will help to explore the medicinal effects of the <em>Abrus precatorius</em> especially in the field of contraception which might be a remarkable activity in this realm. Therefore the present review is aimed to compile up the updated data and highlighting the special features on its pharmacological activities in various diseases.</p>",
"DOI": "10.5455/jabet.2020.d111",
"views": 5349,
"downloads": 266,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/21/178-1577241188-Figure1.jpg",
"caption": "Figure 1. Seed of Abrus precatorius.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/21/178-1577241188-Figure2.jpg",
"caption": "Figure 2. Some chemical compounds for which Abrus precatorius disclaim the medicinal effects.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/21/178-1577241188-Figure3.jpg",
"caption": "Figure 3: Schematic diagram of the sumamry of the chemical constituents and different uses of the leaf , root and seed of Abrus Precatorious.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/21/21/178-1577241188-Figure4.jpg",
"caption": "Figure 4. Schematic diagram of probable mechanism of antifertility or contraceptive effects of Abrus Precatorious.",
"featured": false
}
],
"authors": [
{
"id": 490,
"affiliation": [
{
"affiliation": "Bioresearch laboratory, Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202"
}
],
"first_name": "Sonali",
"family_name": "Bhakta",
"email": "sonali.dvm@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sonali Bhakta, Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Tel.:+8801717620673, Email: sonali.dvm@gmail.com",
"article": 121
},
{
"id": 491,
"affiliation": [
{
"affiliation": "Bioresearch laboratory, Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202"
}
],
"first_name": "Shonkor Kumar",
"family_name": "Das",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 121
}
]
}
]
},
{
"id": 10,
"title": "Vol3 Issue1",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2020",
"slug": "2020-vol3-issue1",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 113,
"slug": "178-1575920248-antidiarrheal-cytotoxic-and-thrombolytic-activities-of-methanolic-extract-of-hedychium-coccineum-leaves",
"type": "original_article",
"manuscript_id": "178-1575920248",
"published": "2020-01-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/37/178-1575920248.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 77-83.",
"academic_editor": "Dr. Md Jamal Uddin, Ewha Womans University, South Korea.",
"cite_info": "Shifah F, Tareq AM, ea tl. Antidiarrheal, cytotoxic and thrombolytic activities of methanolic extract of Hedychium coccineum leaves. J Adv Biotechnol Exp Ther. 2020; 3(1): 77-83.",
"title": "Antidiarrheal, cytotoxic and thrombolytic activities of methanolic extract of Hedychium coccineum leaves",
"abstract": "<p>The study reports the <em>in vivo</em> antidiarrheal and <em>in vitro</em> cytotoxic and thrombolytic activities of methanolic extract of <em>Hedychium coccineum</em> leaves (MEHCL). The antidiarrheal activity was evaluated by castor oil-induced diarrhea, whereas the intestinal motility by charcoal marker. In addition, brine shrimp lethality bioassay and human blood clot lysis were used to evaluate the cytotoxic and thrombolytic activities, respectively. In antidiarrheal study, castor oil-induced diarrhea and gastrointestinal motility exhibited a significant dose dependent reduction in diarrhea and defecation and an extremely significant (P < 0.0001) inhibition in intestinal motility and peristalsis index by 200 and 400 mg/kg of MEHCL. The brine shrimp lethality bioassay revealed a considerable cytotoxic effect of MEHCL (LC<sub>50</sub>= 81.59 µg/mL; R² = 0.927) while in thrombolytic a significant percentage of clot lysis (17.36%, P < 0.01) demonstrated. The findings suggest that <em>H. coccineum</em> leaves could be potential sources for biological activity.</p>",
"DOI": "10.5455/jabet.2020.d110",
"views": 704,
"downloads": 105,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/22/178-1575920248-Figure1.jpg",
"caption": "Figure 1. Cytotoxic effect of methanolic extract of H. coccineum leaves and positive control vincristine sulphate (VCS) on brine shrimp nauplii at different concentration.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/22/178-1575920248-Figure2.jpg",
"caption": "Figure 2. The percentage of clot lysis of methanolic extract of H. coccineum leaves standard drug streptokinase (SK). Results represented in Mean ± SEM (n=5). b P < 0.01, and d P < 0.0001 are statistically significant in comparison to water (Control) followed by unpaired t-test of one-way ANOVA (GraphPad Prism 7).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/22/178-1575920248-Figure3.jpg",
"caption": "Figure 3. Graphical representation of methanolic extract of H. coccineum leaves on antidiarrheal, thrombolytic and cytotoxic activity.",
"featured": false
}
],
"authors": [
{
"id": 450,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Fahmida",
"family_name": "Shifah",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 113
},
{
"id": 451,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Abu Montakim",
"family_name": "Tareq",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 113
},
{
"id": 452,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammed Aktar",
"family_name": "Sayeed",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 113
},
{
"id": 453,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Mohammad Nazmul",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 113
},
{
"id": 454,
"affiliation": [
{
"affiliation": "Drug Discovery, GUSTO A Research Group, Chittagong-4000, Bangladesh"
},
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chittagong-4381, Bangladesh"
}
],
"first_name": "Talha Bin",
"family_name": "Emran",
"email": "talhabmb@gmail.com",
"author_order": 5,
"ORCID": "https://orcid.org/0000-0003-3188-2272",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran PhD, Assistant Professor, Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh. Cell: +88-01819942214, E-mail: talhabmb@gmail.com or talhabmb@bgctub.ac.bd",
"article": 113
},
{
"id": 455,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Md. Ahsan",
"family_name": "Ullah",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 113
},
{
"id": 456,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Muhammad Abdul",
"family_name": "Mukit",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 113
},
{
"id": 457,
"affiliation": [
{
"affiliation": "Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh"
}
],
"first_name": "Ahmed",
"family_name": "Ullah",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 113
}
]
},
{
"id": 110,
"slug": "178-1575398003-presumptive-correlation-between-phenotypic-genotypic-and-symbiotic-diversities-with-antibiotic-susceptibility-traits-of-rhizobial-strains-from-plant-legumes",
"type": "original_article",
"manuscript_id": "178-1575398003",
"published": "2020-01-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/34/178-1575398003.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 59-69.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research Center, Azampur, Dakshinkhan, Uttara, Dhaka 1230, Bangladesh.",
"cite_info": "Shahriar A, Kobra AT, et al. Presumptive correlation between phenotypic, genotypic and symbiotic diversities with antibiotic susceptibility traits of rhizobial strains from plant legumes. J Adv Biotechnol Exp Ther. 2020; 3(1): 59-69.",
"title": "Presumptive correlation between phenotypic, genotypic and symbiotic diversities with antibiotic susceptibility traits of rhizobial strains from plant legumes",
"abstract": "<p>This investigation was carried out to study the extremely diversified characteristics of rhizobial strains isolated from locally produced legumes of different varieties. The rhizobial isolates designated as S 1, S 2, S 3, S 4, S 5 and S 6 were purified and subjected to various morphological, cultural, antibiogram and biochemical and plasmid profiling to unmask their diversified nature to some extent. The study revealed that the Sesbania strain (S 2) possessed extraordinary properties for most other strains. Though the strain is allocated in the slow-growing group, it showed an atypical fast growth rate, and also it produced a profuse amount of exopolysaccharide (EPS)/ lipopolysaccharide (LPS). The Arachis and Vigna strains also showed a faster growth rate than usual, whereas the rate of EPS/LPS production was profuse in the cases of Vigna and Lens strains. This rate was noted low in cases of Pisum and Arachis strains, while Phaseolus produced a moderate amount of EPS/LPS. Like Sesbania, the Pisum strain also absorbed Congo red dye strongly and including Sesbania, all the other strains ware catalase-negative, which was an unusual property of rhizobial strains. Antibiogram profile of the test strains was done to retain experimental strains after plant infection test, as overlapping host range is a common phenomenon in symbiosis. The antibiogram profiles showed that more or less all the strains were resistant to Ampicillin, Bacitracin, and Amoxicillin. Phaseolus strain was intermediate to Bacitracin, and Pisum strain was susceptible to Ampicillin, which can be related to loss of the plasmid. In plasmid profiling of the strains, several numbers of plasmid DNA band was observed under UV transilluminator after gel electrophoresis. In the present study, rhizobial species from different leguminous plants were detected, and plasmids were cured of the cell using acridine orange to determine their effect on Rhizobium spp. for antibiotic resistance. The findings of the research point out the diverse nature of rhizobial isolates with specific reference to the strains infective on Sesbania aculeate, which poses problems to characterize strains infective of different legumes.</p>",
"DOI": "10.5455/jabet.2020.d108",
"views": 986,
"downloads": 154,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/59/21/178-1575398003-Figure1.jpg",
"caption": "Figure 1. A: Growth of the strain on YMA medium infective on Pisum sativum; B: Growth of the strain on YMA medium infective on Sesbania aculeata; C: Growth of the strain on YMA medium infective on Vigna mungo; D: Growth of the strain on YMA medium infective on Phaseolus vulgaris; E: Growth of the strain on YMA medium infective on Lens culinaris; F: Growth of the strain on YMA medium infective on Arachis hypogea.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/59/21/178-1575398003-Figure2.jpg",
"caption": "Figure 2. Growth of test strains on Congo red dye.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/59/21/178-1575398003-Figure3.jpg",
"caption": "Figure 3. Antibiogram profile (zone diameter in mm).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/59/21/178-1575398003-Figure4.jpg",
"caption": "Figure 4. Photograph of the gel under UV illuminator after gel electrophoresis.",
"featured": false
}
],
"authors": [
{
"id": 436,
"affiliation": [
{
"affiliation": "Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka-1217, Bangladesh"
}
],
"first_name": "Asif",
"family_name": "Shahriar",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 110
},
{
"id": 437,
"affiliation": [
{
"affiliation": "Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka-1217, Bangladesh"
},
{
"affiliation": "National Institute of Disease of the Chest and Hospital (NIDCH), Mohakhali, Dhaka-1212, Bangladesh"
}
],
"first_name": "Anina Tul",
"family_name": "Kobra",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 110
},
{
"id": 438,
"affiliation": [
{
"affiliation": "Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka-1217, Bangladesh"
}
],
"first_name": "Farzana Yasmin",
"family_name": "Shomi",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 110
},
{
"id": 439,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chittagong-4381, Bangladesh"
}
],
"first_name": "Talha Bin",
"family_name": "Emran",
"email": "talhabmb@gmail.com",
"author_order": 4,
"ORCID": "https://orcid.org/0000-0003-3188-2272",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Talha Bin Emran PhD, Assistant Professor, Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh. Cell: +88-01819942214, E-mail: talhabmb@gmail.com or talhabmb@bgctub.ac.bd",
"article": 110
},
{
"id": 440,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chittagong-4381, Bangladesh"
}
],
"first_name": "Jewel",
"family_name": "Mallick",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 110
},
{
"id": 441,
"affiliation": [
{
"affiliation": "Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chittagong-4381, Bangladesh"
}
],
"first_name": "Mycal",
"family_name": "Dutta",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 110
}
]
},
{
"id": 111,
"slug": "178-1576005129-seroprevalence-of-brucellosis-among-patients-attending-a-teaching-hospital-in-southern-saudi-arabia",
"type": "original_article",
"manuscript_id": "178-1576005129",
"published": "2020-01-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/19/178-1576005129.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 70-76.",
"academic_editor": "Dr. Hasan-Al-Faruque, Daegu Gyeonbuk Institute of Science and Technology, South Korea.",
"cite_info": "Qamer S, Khan MS, et al. Seroprevalence of brucellosis among patients attending a teaching hospital in southern Saudi Arabia. J Adv Biotechnol Exp Ther. 2020; 3(1): 70-76.",
"title": "Seroprevalence of brucellosis among patients attending a teaching hospital in southern Saudi Arabia",
"abstract": "<p>Human brucellosis, also termed as Malta fever or Mediterranean fever, is prevalent globally having heavy repercussions in the form of reproductive losses and infertility, arthritis, mastitis, and severe pathologic lesions. This research aimed to analyze the seroprevalence of brucellosis in Alkharj region of Saudi Arabia and identify significant risk factors and their impact on prevalence of brucellosis in patients of the region. This research was however confined to investigating the seroprevalence of human brucellosis in such patients that complained prolonged fever. The study used a cross-sectional survey method to identify patients complaining Pyrexia of Unknown Origin (PUO) with tested and proven presence of clinical characteristics of brucellosis. The results confirmed Brucellosis in 38/278(13.6 %) patients and a strong relationship was also observed between its prevalence and the risk factors such as direct contact with animal, consumption of raw milk and animal products. A proactive approach is required to sensitize people about human brucellosis and to exercise severe discipline. The study recommends introducing awareness programs among livestock community and highlight risk factors. Serological surveillance units may also be established at all district headquarters. In order to diagnose the disease at early stages, valid and reliable serological tests should be made readily available.</p>",
"DOI": "10.5455/jabet.2020.d109",
"views": 434,
"downloads": 69,
"figures": [],
"authors": [
{
"id": 442,
"affiliation": [
{
"affiliation": "Department of Microbiology, College Of Medicine, Prince Sattam Bin Abdul Aziz University, Alkharj 11942, Saudi Arabia"
}
],
"first_name": "Shafqat",
"family_name": "Qamer",
"email": "dr.qamar98@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Shafqat Qamer, MBBS, M.Phil, Assistant Professor, Department of Microbiology, College Of Medicine, Prince Sattam Bin Abdul Aziz University, Alkharj 11942, Saudi Arabia, email: dr.qamar98@gmail.com",
"article": 111
},
{
"id": 443,
"affiliation": [
{
"affiliation": "Department of Microbiology, College Of Medicine, Prince Sattam Bin Abdul Aziz University, Alkharj 11942, Saudi Arabia"
}
],
"first_name": "Mohammed Sarosh",
"family_name": "Khan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 111
},
{
"id": 444,
"affiliation": [
{
"affiliation": "Department of Infection control, Civil Hospital Karachi, Pakistan"
}
],
"first_name": "Madiha Sattar",
"family_name": "Ansari",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 111
},
{
"id": 445,
"affiliation": [
{
"affiliation": "Department of Medicine and Infectious disease, Prince Sattam Bin Abdul Aziz University, Alkharj 11942, Saudi Arabia"
}
],
"first_name": "Sana M",
"family_name": "Kamal",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 111
},
{
"id": 446,
"affiliation": [
{
"affiliation": "Department of Biochemistry, College Of Medicine, Al Imam Mohammed Ibn Saud Islamic University, Riyadh, Saudi Arabia"
}
],
"first_name": "Salahuddin",
"family_name": "Khan",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 111
}
]
},
{
"id": 90,
"slug": "178-1572942101-birds-from-pu-luong-nature-reserve-thanh-hoa-province-north-vietnam-an-update-on-biodiversity-and-checklist",
"type": "original_article",
"manuscript_id": "178-1572942101",
"published": "2020-01-19",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/34/178-1572942101.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 29-42.",
"academic_editor": "Dr. Md Jamal Uddin, Ewha Womans University, South Korea.",
"cite_info": "Hoang HN, Nguyen SHL, et al. Birds from Pu Luong Nature Reserve, Thanh Hoa Province, North Vietnam: an update on biodiversity and checklist. J Adv Biotechnol Exp Ther. 2020; 3(1): 29-42.",
"title": "Birds from Pu Luong Nature Reserve, Thanh Hoa Province, North Vietnam: an update on biodiversity and checklist",
"abstract": "<p>Combining use of various methods of bird study from line transect observations, birds capture and release by mist-nets, collection of specimens from local people and analysis of samples kept at Pu Luong Nature Reserve (NR) office throughout the time from 2015 to 2018 and inherit the results of previous studies, we provide an updated list of 252 species of bird belonging to 58 families and 15 orders from Pu Luong NR, Thanh Hoa Province, North Vietnam.. A total of 216 species of birds were recorded during four years. In which, 74 species are reported for the first time from this nature reserve, and 41 species are first records for the avifauna of Thanh Hoa Province. Additional, the data of distribution characters of avifauna in each study site are also provided. According to the International Union for the Conservation of Nature (2019), five species were in the Near Threatened category and three species were in the vulnerable category. Compared to 6 other National Park (NP) and NR in the North Central Coast of Vietnam, the avifauna in Pu Luong NR is close to the avifauna in Pu Hu NR. There are 89 common bird species recorded in all 7 NP and NR, while there are 15 species recorded only in Pu Luong NR. Birds are considered as good bio-indicators of the degree of human disturbance to the forest ecosystem. Observing changes in bird community can assess the status of the forest in the future.</p>",
"DOI": "10.5455/jabet.2020.d104",
"views": 804,
"downloads": 134,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/21/178-1572942101-Figure1.jpg",
"caption": "Figure 1. Pu Luong Nature Reserve in the map of Northern Vietnam (modified from map of Vietnam in Richard Craik & Le Quy Minh, 2018) [4]",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/21/178-1572942101-Figure2.jpg",
"caption": "Figure 2. Map showing the location of bird survey in Pu Luong Nature Reserve map (detail of each sites see the table 1). (modified from map of Pu Luong Nature Reserve).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/21/178-1572942101-Figure3.jpg",
"caption": "Figure 3. Diversity of bird species from areas in the Pu Luong Nature Reserve (detail of each area, see more in Table 1).",
"featured": false
}
],
"authors": [
{
"id": 339,
"affiliation": [
{
"affiliation": "Hong Duc University, 565 Quang Trung Street, Dong Ve Ward, Thanh Hoa City, Vietnam."
},
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy Road, Cau Giay District, Ha Noi, Vietnam."
}
],
"first_name": "Hung Ngoc",
"family_name": "Hoang",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 90
},
{
"id": 340,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy Road, Cau Giay District, Ha Noi, Vietnam."
}
],
"first_name": "Son Hung Lan",
"family_name": "Nguyen",
"email": "sonnlh@hnue.edu.vn",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Son Hung Lan Nguyen, Associate Professor, Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy Road, Cau Giay District, Ha Noi, Vietnam, Email: sonnlh@hnue.edu.vn, Tel.:+84-903212615.",
"article": 90
}
]
},
{
"id": 96,
"slug": "178-1573014843-ameliorating-effects-of-black-seed-oil-on-bisphenol-a-induced-abnormality-of-blood-hormone-profile-and-gonadal-histology-of-female-mice",
"type": "original_article",
"manuscript_id": "178-1573014843",
"published": "2020-01-17",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/27/178-1573014843.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 43-48.",
"academic_editor": "Dr. Md Jamal Uddin, Ewha Womans University, South Korea.",
"cite_info": "Sujan KM, Hoque E, et al. Ameliorating effects of black seed oil on bisphenol A-induced abnormality of blood, hormone profile and gonadal histology of female mice. J Adv Biotechnol Exp Ther. 2020; 3(1): 43-48.",
"title": "Ameliorating effects of black seed oil on bisphenol A-induced abnormality of blood, hormone profile and gonadal histology of female mice",
"abstract": "<p>Bisphenol- A (BPA) is a multi-functional chemical produced in large volumes that is extensively used primarily in the manufacture of polycarbonate plastics and epoxy resins. Black seed oil (BSO) has been employed for many century as one of the main sources of nutrition and healthcare both for humans and animals. The study was carried out to investigate the effects of BPA and BSO on blood, hormone profile, and histopathological changes of ovary in female mice. Total 15 female Swiss Albino mice, aged between 6-8 weeks with an average body weight of 27.4±1g were randomly divided into 3 groups (5 mice/group). Group A act as vehicle control. Group B was administered 50 mg BPA /kg bw daily, while group C received both BPA and BSO and dose rate was 50 mg BPA /kg/day and 1ml BSO/kg/day respectively. Results revealed that Hb concentration, TEC and PCV (p<0.01) were significantly reduced while such difference was absent in other hematological values in BPA treated group compared to control. Inclusion of BSO along with BPA prevented or improved the alterations of those values. Hormonal study showed that, serum thyroxine (T4) and Estradiol level were significantly (p<0.01) reduced by BPA and BSO counteracted the negative effects of BPA. Histopathological study of ovary demonstrated that there were degenerative changes in the lining of granulosa cells of ovarian follicles upon treatment with BPA. From the present study it could be concluded that subjection to BPA is accompanied with anemia, hormonal homeostasis disturbances and granulosa cells degeneration.</p>",
"DOI": "10.5455/jabet.2020.d105",
"views": 1181,
"downloads": 211,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/22/178-1573014843-Figure1.jpg",
"caption": "Figure 1. Photomicrograph (10X) of ovarian histostructure of female mice treated with bisphenol-A (BPA) and black seed oil (BSO). A. Control; B. BPA and C. BPA & BSO. [Scale bar: 5 µm].",
"featured": false
}
],
"authors": [
{
"id": 356,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Khaled Mahmud",
"family_name": "Sujan",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 96
},
{
"id": 357,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Emdadul",
"family_name": "Hoque",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 96
},
{
"id": 358,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Md. Iqramul",
"family_name": "Haque",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 96
},
{
"id": 359,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Afrina",
"family_name": "Mustari",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 96
},
{
"id": 360,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Mohammad Alam",
"family_name": "Miah",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 96
},
{
"id": 361,
"affiliation": [
{
"affiliation": "Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh."
}
],
"first_name": "Md. Kamrul",
"family_name": "Islam",
"email": "k.physiol@bau.edu.bd",
"author_order": 6,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Md. Kamrul Islam, Department of Physiology, Bangladesh Agricultural University, Mymensingh\u0002 2202, Bangladesh, email: k.physiol@bau.edu.bd",
"article": 96
}
]
},
{
"id": 97,
"slug": "178-1574149214-hypoglycemic-and-antidyslipidemic-potential-of-pleurotus-ostreatus-in-streptozotocin-induced-diabetic-rats",
"type": "original_article",
"manuscript_id": "178-1574149214",
"published": "2020-01-16",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/14/178-1574149214.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 49-55.",
"academic_editor": "Dr. Md Nabiul Islam, Yamaguchi University, Japan.",
"cite_info": "Karim MR, Rahman MF, et al. Hypoglycemic and antidyslipidemic potential of Pleurotus ostreatus in streptozotocin-induced diabetic rats. J Adv Biotechnol Exp Ther. 2020; 3(1): 49-55.",
"title": "Hypoglycemic and antidyslipidemic potential of Pleurotus ostreatus in streptozotocin-induced diabetic rats",
"abstract": "<p>Oyster Mushroom (<em>Pleurotusostreatus</em>), an edible mushroom, is traditionally being used as a curing. However, hypoglycemic as well as antidyslipidemic effects on methanolic and ethyl acetate extracts of oyster mushroom in streptozotocin-treated diabetic rat model was not investigated yet. Here, we investigated that oral administration of 200 mg/kg oyster mushroom for 30 days were estimated through evaluating the fasting blood glucose level and blood lipid profile of diabetic rat. The results from our studies explained that <em>P. ostreatus</em>oyster mushroom considerably decreased blood glucose level initiated after treatment on second week. However, oyster mushroom was significantly reduced in plasma triglycerides (TG), total cholesterol (TC), as well as low-density lipoprotein-cholesterol (LDL-C). Additionally, high density lipoprotein-cholesterol (HDL-C) was enhanced by the treatment of oyster mushroom. Therefore, the current study suggests that <em>P. ostreatus</em> has antihyperglycemic and anti-dyslipidemic effect on streptozotocin-induce diabetic rat model.</p>",
"DOI": "10.5455/jabet.2020.d106",
"views": 1182,
"downloads": 134,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/54/22/178-1574149214-Figure1.jpg",
"caption": "Figure 1. STZ induces diabetes which was restored by oyster mushroom treatment in the model of Long Evan rats .",
"featured": false
}
],
"authors": [
{
"id": 362,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, KhwajaYunus Ali University, Enayetpur, Sirajganj, Bangladesh."
}
],
"first_name": "Md. Rezaul",
"family_name": "Karim",
"email": "rezaul929@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Rezaul Karim, email: rezaul929@gmail.com",
"article": 97
},
{
"id": 363,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, KhwajaYunus Ali University, Enayetpur, Sirajganj, Bangladesh."
}
],
"first_name": "Md. Foyzur",
"family_name": "Rahman",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 97
},
{
"id": 364,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, KhwajaYunus Ali University, Enayetpur, Sirajganj, Bangladesh."
}
],
"first_name": "Md. Rezanur",
"family_name": "Rahman",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 97
},
{
"id": 365,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Biotechnology, KhwajaYunus Ali University, Enayetpur, Sirajganj, Bangladesh."
}
],
"first_name": "Zinat",
"family_name": "Tamannaa",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 97
},
{
"id": 366,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh."
}
],
"first_name": "Dipayon Krisna",
"family_name": "Ghose",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 97
},
{
"id": 367,
"affiliation": [
{
"affiliation": "Department of Zoology, University of Rajshahi, Rajshahi, Bangladesh."
}
],
"first_name": "Md. Nurul",
"family_name": "Islam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 97
},
{
"id": 368,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh."
}
],
"first_name": "Md. Tofazzal",
"family_name": "Hossain",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 97
},
{
"id": 369,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh."
},
{
"affiliation": "Global Biotechnology & Biomedical Research Network (GBBRN), Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh."
}
],
"first_name": "Rokibul",
"family_name": "Islam",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 97
},
{
"id": 370,
"affiliation": [
{
"affiliation": "Global Biotechnology & Biomedical Research Network (GBBRN), Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh."
}
],
"first_name": "Md. Ataur",
"family_name": "Rahman",
"email": "ataur1981rahman@hotmail.com",
"author_order": 9,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Ataur Rahman, email: ataur1981rahman@hotmail.com",
"article": 97
}
]
},
{
"id": 94,
"slug": "age-related-development-and-histomorphological-observations-of-bursa-of-fabricius-in-sonali-chicken",
"type": "original_article",
"manuscript_id": "178-1572925770",
"published": "2020-01-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/04/178-1572925770.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 20-28.",
"academic_editor": "Dr. Md Jamal Uddin, Ewha Womans University, South Korea.",
"cite_info": "Ayman U, Alam MR, et al. Age-related development and histomorphological observations of bursa of fabricius in sonali chicken. J Adv Biotechnol Exp Ther. 2020; 3(1): 20-28.",
"title": "Age-related development and histomorphological observations of bursa of fabricius in sonali chicken",
"abstract": "<p>The aim of this research was to investigate the age related histomorphological and involutory changes of bursa of Fabricius of sonali chicken at different postnatal stages in Bangladesh. The present research was carried out on bursa of 25 healthy sonali chicken representing different stage of postnatal life; Day 1, Day 14, Day 28, Day 42 and Day 56. Sample (bursa) was collected after sacrificing the chickens by cervical subluxation method. Harris’s Haematoxylin and 1% Eosin Y stain was done to facilitate microscopic study. Grossly, the bursa was smooth, yellowish to milkish white in color. The average weight gain, length, width and thickness of bursa was developed parallel with age and found statistically significant (<em>p</em><0.001). Age dependent changes were noticed in number of follicle per plicae, length and breadth of plicae, length and breadth of follicle, thickness of cortex and breadth of medulla of follicle, height of lining epithelium and thickness of tunica muscularis and found to be developing significantly (<em>p</em><0.05) from Day 1 to Day 56. No involutory signs were found during study period. From the present study, it might be concluded that the growth and development of bursa of sonali chicken in Bangladesh was age related. The findings of this experiment would help to give an idea about the immune status of sonali chicken and provide a basis for further immunization research at different postnatal stages of development in sonali chicken.</p>",
"DOI": "10.5455/jabet.2020.d103",
"views": 1201,
"downloads": 124,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/22/178-1572925770-Figure1.jpg",
"caption": "Figure 1. A: Gross photographs of the bursa of Fabricius showing located on dorsal aspect of cloaca; B: central lumen (CL) obscured by plicae (P), producing from the bursal wall (W).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/22/178-1572925770-Figure2.jpg",
"caption": "Figure 2. Gross anatomy (average weight; length, width and thickness) of the bursa of Fabricius among different groups, mean ± standard error. **Values with different letter (a,b,c,d,e) within the same line differ significantly (p<0.001).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/22/178-1572925770-Figure3.jpg",
"caption": "Figure 3. Histological architecture of bursa among different groups of postnatal stages. A-Day 1, a:Plicae with follicles,(X10), b:IEL in lining epithelium,(X100); B-Day 14, a:Size of both plicae and follicle increased,(X10), b:CT between follicles and CL between cortex and medulla of follicle,(X40); C-Day 28, a:Plicae and follicle(X10), b:CT and CL, (X40); D-Day 42, a:Large sized follicle within plicae,(X10), b:CT,(X40); E-Day 56, a:Reduction of medullary lymphocyte,(X10), b:UEC in capillary layer,(X100). C-Cortex, M-Medulla, P-Plicae, F-Follicle, L- Lumen, IEL- Intraepithelial lymphocyte, CL-Capillary layer, CT-Connective tissue, UEC-Undifferentiated epithelial cell. H & E stain. Scale bar: 5 µm (X10), 1 µm (X40) and 0.5 µm (X100).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/22/178-1572925770-Figure4.jpg",
"caption": "Figure 4. Analysis of no. of follicles per plicae and length and breadth of plicae; mean ± standard error. **values with different letter (a,b,c,d,e) within the same line differ significantly (p<0.001).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/22/178-1572925770-Figure5.jpg",
"caption": "Figure 5. Analysis of length and breadth of follicle and thickness of cortex and breadth of medulla of follicle of bursa; mean ± standard error. **Values with different letters (a,b,c,d) within the same line differ significantly (p<0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/22/178-1572925770-Figure6.jpg",
"caption": "Figure 6. Analysis of height of lining epithelium and thickness of tunica muscularis of bursa; mean ± standard error. **Values with different letter (a,b,c,d) within the same line differ significantly (p<0.05).",
"featured": false
}
],
"authors": [
{
"id": 350,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Ummay",
"family_name": "Ayman",
"email": "ayman@bau.edu.bd",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-6478-2986",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "UmmayAyman, Lecturer, Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh\u00022202, Bangladesh, Email: ayman@bau.edu.b",
"article": 94
},
{
"id": 351,
"affiliation": [
{
"affiliation": "Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Rafiqul",
"family_name": "Alam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 94
},
{
"id": 352,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Shonkor Kumar",
"family_name": "Das",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 94
}
]
},
{
"id": 100,
"slug": "178-1576634495-first-two-years-journey-journal-of-advanced-biotechnology-and-experimental-therapeutics",
"type": "editorial_article",
"manuscript_id": "178-1576634495",
"published": "2020-01-07",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/24/178-1576634495.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 56-58.",
"academic_editor": "Dr. Md Jamal Uddin, Ewha Womans University, South Korea.",
"cite_info": "Rahman MS, Rahman MA. First two years journey: Journal of Advanced Biotechnology and Experimental Therapeutics. J Adv Biotechnol Exp Ther. 2020; 3(1): 56-58.",
"title": "First two years journey: Journal of Advanced Biotechnology and Experimental Therapeutics",
"abstract": "",
"DOI": "10.5455/jabet.2020.d107",
"views": 235,
"downloads": 72,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/58/23/178-1576634495-Figure1.jpg",
"caption": "Figure 1. Summary of progress by JABET in the first two years.",
"featured": false
}
],
"authors": [
{
"id": 426,
"affiliation": [
{
"affiliation": "Department of Animal Science and Technology and BET Research Institute, Chung-Ang University, Anseong, Gyeonggi-do 17546, Republic of Korea."
},
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakshinkhan, Uttara, Dhaka-1230, Bangladesh."
}
],
"first_name": "Md Saidur",
"family_name": "Rahman",
"email": "shohagvet@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-4583-8731",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Saidur Rahman, E-mail: shohagvet@gmail.com, Fax: 82-31-670-3019, Telephone: 82-31-670-4841.",
"article": 100
},
{
"id": 427,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakshinkhan, Uttara, Dhaka-1230, Bangladesh."
},
{
"affiliation": "Center for Neuroscience, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea."
}
],
"first_name": "Md. Ataur",
"family_name": "Rahman",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 100
}
]
},
{
"id": 86,
"slug": "178-1570099246-a-comparative-review-on-tlqp-21-receptors-rodent-versus-human",
"type": "review_article",
"manuscript_id": "178-1570099246",
"published": "2020-01-06",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/20/178-1570099246.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 09-19.",
"academic_editor": "Dr. Md Murshidul Ahsan, National University of Singapore, Singapore.",
"cite_info": "Akhter MS, A comparative review on TLQP-21 receptors: rodent versus human. J Adv Biotechnol Exp Ther. 2020; 3(1): 09-19.",
"title": "A comparative review on TLQP-21 receptors: rodent versus human",
"abstract": "<p><strong> </strong>Growing functional information regarding bioactive TLQP-21 has led to the ubiquitous demand for identification of receptors associated to the peptide, which resulted in the first wave of murine TLQP-21 receptors, gC1qR and C3AR1. gC1qR was identified as receptor of TLQP-21 using chemical crosslinking and monomeric avidin column purification following by MS analysis. TLQP-21 responsive CHO-K1 cells were used to search for its receptor, C3AR1. Putative GPCRs, which may partake in regulating intracellular biological functions induced by TLQP-21, were indexed after the CHO-K1 cellular transcriptome was sequenced using unbiased Genome Wide Sequencing. TLQP-21 binding in the cells were found to be reduced by the gene knockdown with the siRNAs targeting C3AR1. C3AR1 antagonist, SB290157 was shown to prohibit TLQP-21 activity in CHOK1 cells. But this finding was not demonstrable in human cell line. The differences of human TLQP-21 sequence with that of murine TLQP-21 explains the poor binding of the human orthologue with its corresponding receptor. This may suggest a different set of receptors when considering human and rodent variants of TLQP-21. The identification of HSPA8 as receptor was performed using affinity based chromatography and mass spectrometry from human SHSY-5Y cells. Molecular studies <em>in silico</em> revealed that the peptide binding pocket in HSPA8 is an appropriate fit for TLQP-21 docking. Cross-linking and FACS methods presented the TLQP-21 binding to cells from the SHSY-5Y line. The establishment of HSPA8 as a putative receptor for human TLQP-21 can be exploited to explore new horizon in diagnosis and therapies for VGF related human diseases.</p>",
"DOI": "10.5455/jabet.2020.d102",
"views": 528,
"downloads": 98,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/23/178-1570099246-Figure1.jpg",
"caption": "Figure 1. Diagrammatic representation of the effects of TLQP-21 binding to its receptors (Modified from Cero et al., 2014) [25].",
"featured": false
}
],
"authors": [
{
"id": 327,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh."
}
],
"first_name": "Md. Shamim",
"family_name": "Akhter",
"email": "shamim11akhter@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md. Shamim Akhter, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh, Email: shamim11akhter@gmail.com, Tel.: +88-01718482468",
"article": 86
}
]
},
{
"id": 80,
"slug": "178-1570132696-hmg-coa-reductase-inhibitor-rosuvastatin-averted-carbon-tetrachloride-induced-oxidative-stress-inflammation-and-fibrosis-in-the-liver-of-rats",
"type": "original_article",
"manuscript_id": "178-1570132696",
"published": "2019-01-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/17/178-1570132696.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2020; 3(1): 01-08.",
"academic_editor": "Dr. Md Jamal Uddin, Ewha Womans University, South Korea.",
"cite_info": "Sikder B, Akter F, et al. HMG-CoA reductase inhibitor, rosuvastatin averted carbon tetrachloride-induced oxidative stress, inflammation and fibrosis in the liver of rats. J Adv Biotechnol Exp Ther. 2020; 3(1): 01-08.",
"title": "HMG-CoA reductase inhibitor, rosuvastatin averted carbon tetrachloride-induced oxidative stress, inflammation and fibrosis in the liver of rats",
"abstract": "<p><strong> </strong>The aim of this study was to examine the effect of rosuvastatin in experimentally-induced hepatic inflammation and fibrosis in rats. Carbon tetra chloride (CCl4) was administered orally to induce liver damage in female Long Evans rats. Rats were treated with CCl4 alone twice a week over two weeks. Rosuvastatin (10 mg/kg) was also given daily to CCl4 treated rats concurrently by nasogastric gavage. After two weeks, various oxidative stress markers as well as liver markers enzymes were investigated in different animal groups tested in this study. Moreover, histological assessments were also done for inflammatory cell infiltration and fibrosis in the liver of all test groups. Plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities were increased in the CCl4 group compared with the control group. Increased liver enzyme activities were significantly decreased by rosuvastatin treatment. Moreover, rosuvastatin treatment inhibited the formation of lipid peroxidation products in CCl4 administered rats. Rosuvastatin treatment also restored the decreased superoxide dismutase (SOD) activities as well as elevated the reduced glutathione concentration in CCl4 administered rats. Liver tissues from rats of control group also revealed no significant pathological changes, while CCl4 administered rats showed significant infiltration of inflammatory cells and liver fibrosis, which was further, normalized or significantly decreased by rosuvastatin treatment. This study revealed that, rosuvastatin treatment may ameliorate all necro-inflammatory and fibrotic changes in liver tissues of CCl4 induced rats and could be used as an alternative therapy for chemical or drug-induced liver fibrosis.</p>",
"DOI": "10.5455/jabet.2020.d101",
"views": 1204,
"downloads": 188,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/23/178-1570132696-Figure1.jpg",
"caption": "Figure 1: Effect of rosuvastatin on liver marker enzyme function in CCl4 administered rats. Data are presented as mean±SEM, n=6. Statistical analysis was done by One Way ANOVA followed by Newman-Keuls post hoc test. Statistical significance was considered as p<0.05 in all cases.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/23/178-1570132696-Figure2.jpg",
"caption": "Figure 2: Effect of rosuvastatin on oxidative stress markers in CCl4 administered rats. Data are presented as mean±SEM, n=6. Statistical analysis was done by One Way ANOVA followed by Newman-Keuls post hoc test. Statistical significance was considered as p<0.05 in all cases.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/23/178-1570132696-Figure3.jpg",
"caption": "Figure 3: Effect of rosuvastatin on antioxidant enzyme function and inflammatory markers in CCl4 administered rats. Data are presented as mean±SEM, n=6. Statistical analysis was done by One Way ANOVA followed by Newman-Keuls post hoc test. Statistical significance was considered as p<0.05 in all cases.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/09/23/178-1570132696-Figure4.jpg",
"caption": "Figure 4: Effect of rosuvastatin on hepatic inflammation (upper panel) and fibrosis (lower panel) in CCl4 administered rats. A, E- control; B, F- Control+rosuvastatin; C, G- CCl4 and D, H- CCl4+rosuvastatin. Magnification X40.",
"featured": false
}
],
"authors": [
{
"id": 291,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Biswajit",
"family_name": "Sikder",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 80
},
{
"id": 292,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Farzana",
"family_name": "Akter",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 80
},
{
"id": 294,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Anayt",
"family_name": "Ulla",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 80
},
{
"id": 293,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Nusrat",
"family_name": "Subhan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 80
},
{
"id": 295,
"affiliation": [
{
"affiliation": "Pharmacy Discipline, Khulna University, Bangladesh"
}
],
"first_name": "Md. Iqbal",
"family_name": "Ahmed",
"email": "i.ahmed@pharm.ku.ac.bd",
"author_order": 5,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Md. Iqbal Ahmed, Associate Professor, Pharmacy Discipline, Khulna University, Bangladesh, Email: i.ahmed@pharm.ku.ac.bd",
"article": 80
},
{
"id": 296,
"affiliation": [
{
"affiliation": "Department of Pharmaceutical Sciences, North South University, Bangladesh"
}
],
"first_name": "Md Ashraful",
"family_name": "Alam",
"email": "ashraful.alam@northsouth.edu",
"author_order": 6,
"ORCID": "https://orcid.org/0000-0001-7596-5868",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Md Ashraful Alam, Associate Professor, Department of Pharmaceutical Sciences, North South University, Bangladesh, Email: ashraful.alam@northsouth.edu",
"article": 80
}
]
}
]
}
],
"title": "2020",
"slug": "2020",
"order": 3
},
{
"id": 5,
"issues": [
{
"id": 9,
"title": "Vol2 Issue3",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2019",
"slug": "2019-vol2-issue3",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 77,
"slug": "178-1568893361-comparable-preventive-effects-of-laboratory-grown-spirulina-and-market-spirulina-against-arsenic-induced-alterations-in-the-liver-of-adult-rats",
"type": "original_article",
"manuscript_id": "178-1568893361",
"published": "2019-09-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/25/178-1568893361.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(3): 146-152.",
"academic_editor": "Dr. Hasan-Al-Faruque, Daegu Gyeonbuk Institute of Science and Technology, South Korea.",
"cite_info": "Khair A, Awal MA, et al. Comparable preventive effects of laboratory-grown spirulina and market spirulina against arsenic-induced alterations in the liver of adult rats. J Adv Biotechnol Exp Ther. 2019; 2(3): 146-152.",
"title": "Comparable preventive effects of laboratory-grown spirulina and market spirulina against arsenic-induced alterations in the liver of adult rats",
"abstract": "<p>Arsenic (As) is a naturally occurring ubiquitous environmental toxicant. It has been reported that spirulina has protective effects against As toxicity. In the present study, we compared the prophylactic effects of spirulina [laboratory grown agro-based spirulina (Ab-Sp) and market spirulina (M-Sp)] against the histopathological changes in liver induced by inorganic arsenic (iAs) in male rats. Three doses (1.0g, 1.5g and 2.0g/kg feed) of both the Ab-Sp and M-Sp with feed and 3.0mg NaAsO<sub>2</sub>/kg body weight (BW) in drinking water were given simultaneously to six groups (T4, T5, T6, T7, T8 and T9) of rats daily for 90 days. Same dose of NaAsO<sub>2</sub> (3.0mg/kg; T1) and highest dose (2.0g/kg) of each of Ab-Sp (T2) and M-Sp (T3) were given individually to other 3 groups keeping the rest one as control (T0) with normal feed and water. As feeding resulted in a variety of histopathological changes in liver, including congestion in the central veins, hemorrhage in the hepatic lobules and lobular tissues, higher numbers of hypertrophic hepatocytes with hypertrophic nucleus, hepatocytes with visible chromatin in the nucleus and vacuolated hepatocytes. The Ab-Sp treatment successfully improved all the histopathological conditions. In contrast, the M-Sp improved the conditions by combating all the histopathological conditions including vacuolated hepatocytes, erosions and hemorrhages in the liver. Taken together, the spirulina was found as an effective agent in prevention of the histopathological changes while we first clarified that Ab-Sp had better result than the M-Sp and finally, 2.0g Ab-Sp/kg feed was found as the best dose.</p>",
"DOI": "10.5455/jabet.2019.d38",
"views": 772,
"downloads": 124,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/14/28/178-1568893361-Figure1.jpg",
"caption": "Figure 1. Representative photomicrographs of H & E stained histological section of liver of group T0 (A) and T1 (C). B and D are the enlargement of CV areas in A and B, respectively. Black arrows indicate nucleus with visible chromatin, White arrows indicate mitotic phase, black arrowheads indicate vacuolated hepatocytes, double arrowheads indicates congestion and hemorrhage in the hepatic matrix, white arrowheads indicates hypertrophic hepatocytes with hypertrophic nucleus. CV, central veins. Scale bar in A, C = 100 μm; B, D= 10μ m.",
"featured": false
}
],
"authors": [
{
"id": 268,
"affiliation": [
{
"affiliation": "Department of Livestock Services, Bangladesh"
},
{
"affiliation": "Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, Bangladesh"
}
],
"first_name": "Abul",
"family_name": "Khair",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 77
},
{
"id": 269,
"affiliation": [
{
"affiliation": "Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, Bangladesh"
}
],
"first_name": "Md Abdul",
"family_name": "Awal",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 77
},
{
"id": 270,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md Zahirul Islam",
"family_name": "Khan",
"email": "zahirul@umk.edu.my",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Zahirul Islam Khan, Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh, Bangladesh , Email: zahirul@umk.edu.my",
"article": 77
},
{
"id": 288,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh, Bangladesh"
}
],
"first_name": "Md Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 77
},
{
"id": 289,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh, Bangladesh"
},
{
"affiliation": "Division of Neuroanatomy, Yamaguchi University School of Medicine, Ube, Japan"
}
],
"first_name": "Mir Rubayet",
"family_name": "Jahan",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 77
},
{
"id": 290,
"affiliation": [
{
"affiliation": "Division of Neuroanatomy, Yamaguchi University School of Medicine, Ube, Japan"
}
],
"first_name": "Md Nabiul",
"family_name": "Islam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 77
}
]
},
{
"id": 75,
"slug": "178-1566176524-analysis-of-maize-profilin-4-isoform-as-an-allergen",
"type": "original_article",
"manuscript_id": "178-1566176524",
"published": "2019-09-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/57/178-1566176524.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(3): 134-139.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research Center, Azampur, Dhaka 1230, Bangladesh.",
"cite_info": "Alam S, Hasan MK, et al. Analysis of maize profilin-4 isoform as an allergen. J Adv Biotechnol Exp Ther. 2019; 2(3): 134-139.",
"title": "Analysis of maize profilin-4 isoform as an allergen",
"abstract": "<p>Profilin is an actin monomer-binding protein that controls the dynamic turnover of actin filaments and is ubiquitously present in different organisms ranging from prokaryotes to higher eukaryotes. Maize (<em>Zea mays</em>) profilin-4 isoform is a pollen-specific protein. Birch profilin isoform is a known allergen but maize profilin is yet to be characterized. In this study, we investigated the properties of maize profilin-4 isoform’s allergenicity. To this end, we first analyzed profilin-4 isoform’s physicochemical properties, including molecular weight (~14kD), theoretical pI (4.63), and amino acids composition; and found that it might have allergenic potency. Then we tested the potential B cell epitope candidates using different immune-informatics tools housed at IEDB analysis resource. For the B cell epitope prediction, potential antigenic sites on the protein surface were predicted by both propensity scale and machine learning method followed by their mapping of 3D structure prediction. Our findings suggest that profilin-4 isoform is a potential allergen and can induce allergic responses.</p>",
"DOI": "10.5455/jabet.2019.d36",
"views": 796,
"downloads": 134,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/28/178-1566176524-Figure1.jpg",
"caption": "Figure 1: The amino acids composition in profilin-4. Glycine (Gly) and Asparagine (Asn) are the major (14.5%) and the least (~0.8%) constituents, respectively.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/28/178-1566176524-Figure2.jpg",
"caption": "Figure 2: Kolaskar and Tongaonkar antigenicity graphical plot. The protein sequences that satisfied the set antigenic propensity threshold value of 1.00 are predicted to be potential antigenic region.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/28/178-1566176524-Figure3.jpg",
"caption": "Figure 3: Parker hydrophilicity plot. The x-axis represents the amino acid position and the y-axis represents the hydrophilicity score.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/28/178-1566176524-Figure4.jpg",
"caption": "Figure 4: Potential B cell epitopes predicted from BepiPred tool. Threshold value for potential epitope was set 0.350. Regions shaded with yellow colour are predicted as potential epitopes. The maximum score predicted here is 1.630.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/28/178-1566176524-Figure5.jpg",
"caption": "Figure 5: 3D structure and its validation using the Ramachandran plot for profilin-4 protein. (A) Cartoon representation of the predicted structure of the profilin-4 protein. This image has been developed using Swiss PDB viewer tool (B) amino acid residues are distributed in the Ramachandran plot.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/28/178-1566176524-Figure6.jpg",
"caption": "Figure 6: Mapping of B cell epitopes on the 3D structure of the profilin-4. BepiPred predicted epitopes are mapped on the surface of the profilin-4 protein structure where B cell epitopes are highlighted in red (epitope-1), olive (epitope-2), green (epitope-3) and yellow (epitope-4) and the rest of the non-reactive portion highlighted in pink.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/28/178-1566176524-Figure7.jpg",
"caption": "Figure 7: World corn production from 1988 to 2018. This bar diagram shows a gradual increase in corn production over three decades around the world. (Modified from https://www.indexmundi.com/agriculture/?country=us&commodity=corn&graph=production).",
"featured": false
}
],
"authors": [
{
"id": 259,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Saruar",
"family_name": "Alam",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 75
},
{
"id": 260,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh"
}
],
"first_name": "Md. Kamrul",
"family_name": "Hasan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 75
},
{
"id": 261,
"affiliation": [
{
"affiliation": "Department of Biology, St. John's University, Queens, New York 11439"
}
],
"first_name": "Md. Faruk",
"family_name": "Hossain",
"email": "farukbmb16@gmail.com",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0002-3457-7903",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Department of Biology, St. John's University, Queens, New York 11439\r\n E-mail: farukbmb16@gmail.com",
"article": 75
}
]
},
{
"id": 76,
"slug": "178-1565175151-phytochemical-profiling-and-antioxidant-potentiality-of-medicinal-plants-along-with-their-antibacterial-efficacy",
"type": "original_article",
"manuscript_id": "178-1565175151",
"published": "2019-09-25",
"pdf_file": null,
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(3): 140-145.",
"academic_editor": "Dr. Md. Atikur Rahman, National Institute of Animal Science, South Korea",
"cite_info": "Ahmed SR, Romi IJ, et al. Phytochemical profiling and antioxidant potentiality of medicinal plants along with their antibacterial efficacy. J Adv Biotechnol Exp Ther. 2019; 2(3): 140-145.",
"title": "Phytochemical profiling and antioxidant potentiality of medicinal plants along with their antibacterial efficacy",
"abstract": "<p><strong> </strong>The aim of this study was to explore phytochemical profiling, antioxidant and antibacterial activity of four medicinal plants including <em>Catharanthus roseus, Aegle marmelos, Moringa oleifera, </em>and <em>Ageratum conyzoids</em> grown in Sylhet district, Bangladesh. In this study, total 11 phytochemicals were screened from methanol extract of four medicinal plants, wherein flavonoid, tannin, sterol, phenol were present in all four medicinal plants. <em>In vitro</em>, antioxidant activity of these medicinal plants extract was investigated by DPPH-radical scavenging assay. The <em>Aegle marmelos</em> exhibited the highest antioxidant activity followed by <em>Moringa oleifera, Ageratum conyzoids, </em>and <em>Catharanthus roseus extract</em>. Methanolic extracts of same medicinal plants were subjected to a test of their antibacterial activities against <em>Staphylococcus aureus, Escherichia coli, Klebsiella sp.<strong>, </strong>Pseudomonas sp .and Salmonella sp</em>. by agar disc diffusion method. The highest antibacterial potential was observed in the extract of <em>Aegle marmelos </em>against <em>Salmonella sp</em>. followed by <em>Catharanthus roseus against Pseudomonas sp .</em>with zone of inhibition of 18.67 mm, 15.0 mm, respectively. This study confirmed the efficacy of some native medicinal plants extract as potential source of phytochemicals, along with natural antioxidant and antimicrobials, which provide new possibilities to employing them against disease causing test organisms.</p>",
"DOI": "10.5455/jabet.2019.d37",
"views": 1701,
"downloads": 0,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/28/178-1565175151-Figure1.jpg",
"caption": "Figure 1. Antibacterial activity of a) Ageratum conyzoids against S. aureus, b) Moringa oleifera against Klebsiella sp.",
"featured": false
}
],
"authors": [
{
"id": 262,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Plant and Environmental Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Sheikh Rashel",
"family_name": "Ahmed",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 76
},
{
"id": 263,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Ismat Jahan",
"family_name": "Romi",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 76
},
{
"id": 264,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Jamil",
"family_name": "Ahmed",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 76
},
{
"id": 265,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Pharmaceuticals and Industrial Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Mahmudul",
"family_name": "Hasan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 76
},
{
"id": 266,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Rubel",
"family_name": "Roy",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 76
},
{
"id": 267,
"affiliation": [
{
"affiliation": "Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
},
{
"affiliation": "Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet-3100, Bangladesh."
}
],
"first_name": "Mohammad Mehedi Hasan",
"family_name": "Khan",
"email": null,
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-4814-3065",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Mehedi Hasan Khan, Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet\u00023100, Bangladesh. E-mail: mehedi2001bdbd@gmail.com, Tel.: +8801680592166",
"article": 76
}
]
},
{
"id": 74,
"slug": "178-1565811282-isolation-and-characterization-of-osmophilic-fermentative-yeasts-from-bangladeshi-honeys",
"type": "original_article",
"manuscript_id": "178-1565811282",
"published": "2019-09-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/22/178-1565811282.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(3): 127-133.",
"academic_editor": "Dr. Md Murshidul Ahsan, National University of Singapore, Singapore.",
"cite_info": "Mukti RF, Chowdhury MMK, et al. Isolation and characterization of osmophilic fermentative yeasts from Bangladeshi honeys. J Adv Biotechnol Exp Ther. 2019; 2(3): 127-133.",
"title": "Isolation and characterization of osmophilic fermentative yeasts from Bangladeshi honeys",
"abstract": "<p>Despite the medicinal values and economic importance, honey is also a source of osmophilic fermentative yeasts. So, it can be utilized for the isolation of local fermentative yeast. Here, 1ml honey was inoculated in yeast extract peptone dextrose (YPD) broth containing 4% dextrose. After overnight incubation, the broth was streaked on an YPD agar plate to purify yeast colonies. In this study, total eight strains were isolated from seven physio-chemically defined florally diversified honey samples of Bangladesh. Microscopic morphology, plasmid profile, growth pattern and fermentative capacity of these isolates were determined. Under light microscope, these yeasts had one of three distinct shapes: ovoid, spherical, or cylindrical. The cytoplasm in young, actively reproducing cells occupied most of the interior and looked homogeneous. Two plasmids of around 3kb and 2.1 kb were common in these strains except for one. While compared with Baker’s yeast, these strains showed faster growth. Five of them were attributed to high fermentative potency. Yeast 2 showed the highest fermentative potency yielding 33.48% (v/v) ethanol. We suggest that these strains have potentialities for efficient bioethanol production to meet the increasing demand of biofuel.</p>",
"DOI": "10.5455/jabet.2019.d35",
"views": 641,
"downloads": 119,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/29/178-1565811282-Figure1.jpg",
"caption": "Figure 1. Microscopic images of the yeast isolates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/29/178-1565811282-Figure2.jpg",
"caption": "Figure 2. Yeast cells become elongated and cylindrical with elliptical edges to split evenly into two daughter cells by fission in old cultures (A). Chain of yeast cells (pseudomycelium) produced by budding in young cultures (B).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/29/178-1565811282-Figure3.jpg",
"caption": "Figure 3. Comparison of the growth curves of the yeast isolates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/29/178-1565811282-Figure4.jpg",
"caption": "Figure 4. Fermentation capacity of the yeast isolates expressed as percent (v/v) of produced ethanol.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/29/178-1565811282-Figure5.jpg",
"caption": "Figure 5. Agarose gel electrophoresis of the isolated plasmids from the yeast isolates.",
"featured": false
}
],
"authors": [
{
"id": 256,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, East West University, Aftabnagar, Dhaka-1212"
}
],
"first_name": "Roushney Fatima",
"family_name": "Mukti",
"email": "roushney@ewubd.edu",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-7059-8165",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Roushney Fatima Mukti, Department of Genetic Engineering and Biotechnology, East West University, A/2, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh, Email: roushney@ewubd.edu , Tel.: +8801719273371",
"article": 74
},
{
"id": 257,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000"
}
],
"first_name": "Md. Miraj Kobad",
"family_name": "Chowdhury",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 74
},
{
"id": 258,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000"
}
],
"first_name": "Md. Aftab",
"family_name": "Uddin",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 74
}
]
},
{
"id": 69,
"slug": "178-1563256003-development-of-high-frequency-in-vitro-plant-regeneration-protocol-of-brassica-napus",
"type": "original_article",
"manuscript_id": "178-1563256003",
"published": "2019-09-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/41/178-1563256003.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(3): 114-119.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research Center, Dhaka-1230, Bangladesh.",
"cite_info": "Dina MMA, Sultana S, et al. Development of high frequency in vitro plant regeneration protocol of Brassica napus. J Adv Biotechnol Exp Ther. 2019; 2(3): 114-119.",
"title": "Development of high frequency in vitro plant regeneration protocol of Brassica napus",
"abstract": "<p>Various factors like plant growth regulator combinations, explant type and explant age were examined for establishing a convenient protocol for high frequency plant regeneration of <em>Brassica napus</em>. Cotyledon and hypocotyl explants of <em>B. napus</em> cv. BARI sarisha-8 were cultured on Murashige and Skoog (MS) medium fortified by different strengths of 6-Benzylaminopurine (BA), 2,4-Dichlorophenoxy Acetic Acid (2,4-D) and α-Napthalene Acetic Acid (NAA) to determine the suitable callus induction and shoot regeneration media. MS medium supplemented with 0.5 mg/L NAA and 3.0 mg/L BA was the best combination as regeneration medium because of showing highest frequency for both callus (80% for cotyledon and 53.33% for hypocotyl explants) and shoot initiation (73.33% for cotyledon and 40% for hypocotyl explants). Four days old cotyledon explants showed the highest (73.33%) frequency of shoot regeneration and highest shoot number to each explant (3.13) when 3-7 days old cotyledon cultured. Among the four tested genotypes of <em>B. napus</em>, BARI sarisha-8 showed the highest shoot regeneration frequency (73.33%) with maximum shoot number to each explant (3.13) while the lowest frequency of shoot regeneration was found in BINA sarisha-4 (46.66%) with minimum shoot number per explant (1.66). The ideal rooting medium was MS media comprised with 0.1 mg/L NAA that offered the maximum frequency (100%) of rooting. The regenerated plantlets were shifted to pot soil, acclimatized and grown until maturity in natural conditions. All plants were fertile and morphologically identical with the source plants. This protocol for high frequency regeneration of <em>B. napus</em> could be used for genetic transformation experiments.</p>",
"DOI": "10.5455/jabet.2019.d33",
"views": 701,
"downloads": 64,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/29/178-1563256003-Figure1.jpg",
"caption": "Figure 1. In vitro organogenesis of B. napus cv. BARI sarisha-8. (a) cotyledon explants at 1st day of culture, (b) hypocotyl explants at 1st day of culture, (c) callus and shoot induction from cotyledon explants after 14 days of culture on regeneration media (MS + 0.5 mg/L NAA + 3.0 mg/L BA), (d) callus and shoot induction from hypocotyl explants after 14 days of culture on regeneration media (MS + 3.0 mg/L BA + 0.5 mg/L NAA), (e) shoot elongation on shoot induction medium from cotyledon explants, (f) shoot elongation from hypocotyl explants on shoot induction medium, (g, h) root induction on MS + 0.1 mg/L NAA in regenerated shoots obtained from cotyledon and hypocotyl explants, respectively, (i) flowered plants in natural environment. Scale bars represent 5mm (a & b), 1cm (c, d, e, f, g & h), 10cm (i).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/29/178-1563256003-Figure2.jpg",
"caption": "Figure 2. Effects of explant age on shoot regeneration of B. napus cv. BARI sarisha-8 from cotyledon explant. Data consist of three replications and 10 explants were used for each replication. Bars represent SD of means. Values with distinct letters are significantly diverse at P = 0.05 (DMRT).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/29/178-1563256003-Figure3.jpg",
"caption": "Figure 3. Influence of genotypes on shoot regeneration from 4 days old cotyledon explants of B. napus. Data consist of three replications and 10 explants were used for each replication. Bars represent SD of means. Values with distinct letters are significantly diverse at P = 0.05 (DMRT).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/29/178-1563256003-Figure4.jpg",
"caption": "Figure 4. Root development of regenerated shoots from cotyledon explants of B. napus cv. BARI sarisha-8. Data consist of three replications and 10 regenerated plants were used for each replication. Bars represent SD of means. Values with distinct letters are significantly diverse at P = 0.05 (DMRT).",
"featured": false
}
],
"authors": [
{
"id": 242,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Mst Maiful Akter",
"family_name": "Dina",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 69
},
{
"id": 243,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Sayeda",
"family_name": "Sultana",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 69
},
{
"id": 244,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Mohammed Shafi Ullah",
"family_name": "Bhuiyan",
"email": "msubhuiyan@gmail.com",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammed Shafi Ullah Bhuiyan, Associate Professor, Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh, Email: msubhuiyan@gmail.com , Tel.: +880-1727618980.",
"article": 69
}
]
},
{
"id": 72,
"slug": "178-1565245703-biochemical-and-molecular-identification-of-antibiotic-producing-bacteria-from-waste-dumpsite-soil",
"type": "original_article",
"manuscript_id": "178-1565245703",
"published": "2019-09-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/21/178-1565245703.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(3): 120-126.",
"academic_editor": "Dr. Md. Niamul Haque, ABEx Bio-Research Center, Dhaka-1230, Bangladesh.",
"cite_info": "Mandal C, Tabassum T, et al. Biochemical and molecular identification of antibiotic-producing bacteria from waste dumpsite soil. J Adv Biotechnol Exp Ther. 2019; 2(3): 120-126.",
"title": "Biochemical and molecular identification of antibiotic-producing bacteria from waste dumpsite soil",
"abstract": "<p>Antibiotics are the secondary metabolites produced by bacteria and fungi to defend themselves from other pathogens. These secondary metabolites are being produced and used as a drug to cure different diseases. However, antibiotic resistance is a common problem that demands an urgent need to discover new antibiotics routinely. Several approaches have been performed to develop novel and potent antibiotics from natural sources against pathogenic bacteria. Among the different sources soil has been considered as the potent natural source of obtaining bacteria with the ability to produce novel antibiotics. The present work has been focused on the isolation of antibiotic producing bacteria from the soil samples collected from waste dumpsite. Among the 5 microbial isolates, 2 were shown to have inhibitory activities against <em>Escherichia coli</em> and <em>Salmonella paratyphi</em>. Morphological and biochemical tests revealed that both strains were <em>Bacillus </em>species with some differences in cultural characteristics. Molecular identification was performed by sequencing of the amplified 16S rRNA PCR products. The result showed that the two microbes were <em>Bacillus subtilis</em> and <em>Bacillus cereus</em> with 98% and 97% similarity score, respectively. This study suggests that <em>Bacillus</em> species have the potential to produce antibiotics against a broad spectrum of microbial growth and will be helpful in improving these strains for better production.</p>",
"DOI": "10.5455/jabet.2019.d34",
"views": 1813,
"downloads": 154,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/29/178-1565245703-Figure1.jpg",
"caption": "Figure 1. Visualisation of morphology of BI-2 and BI-3 using Gram staining. The rod shaped bacilli are clearly visible with endospores.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/44/29/178-1565245703-Figure2.jpg",
"caption": "Figure 2. Evolutionary relationships of different taxa. The evolutionary history was inferred using the Neighbor-Joining method in MEGA7. A) and B) represents the phylogenetic tree of bacterial isolate BI-2 and BI-3, respectively. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) is shown next to the branches. The evolutionary distances were computed using the number of differences method and are in the units of the number of base differences per sequence. The analysis involved 10 nucleotide sequences. All positions containing gaps and missing data were eliminated. The box indicates deposited sequences of BI-2 and BI-3.",
"featured": false
}
],
"authors": [
{
"id": 245,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh"
}
],
"first_name": "Chanchal",
"family_name": "Mandal",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 72
},
{
"id": 246,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh"
}
],
"first_name": "Tasmina",
"family_name": "Tabassum",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 72
},
{
"id": 247,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh"
}
],
"first_name": "Md. Jamil",
"family_name": "Shuvo",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 72
},
{
"id": 248,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh"
}
],
"first_name": "Ahsan",
"family_name": "Habib",
"email": "ahsan_habib@ku.ac.bd",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Ahsan Habib, PhD, Associate Professor, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh, E-mail: ahsan_habib@ku.ac.bd , Tel.: +88041720171 Ex. 2027",
"article": 72
}
]
},
{
"id": 68,
"slug": "178-1560250921-optimization-of-production-and-partial-characterization-of-cellulase-and-protease-enzymes-from-aeromonas-hydrophila-asm-s32",
"type": "original_article",
"manuscript_id": "178-1560250921",
"published": "2019-09-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/45/178-1560250921.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(3): 103-113.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research, Dhaka-1230, Bangladesh.",
"cite_info": "Chakraborty S, Joy ZF, et al. Optimization of production and partial characterization of cellulase and protease enzymes from Aeromonas hydrophila ASM-S32. J Adv Biotechnol Exp Ther. 2019; 2(3): 103-113.",
"title": "Optimization of production and partial characterization of cellulase and protease enzymes from Aeromonas hydrophila ASM-S32",
"abstract": "<p>Microbial hydrolytic enzymes, especially cellulase and protease, are widely used in industrial processes due to their low cost, large productivity, chemical stability, environmental protection, plasticity and vast availability. But existing cellulase and protease enzymes are not capable enough to fulfill the industrial demands due to the lack of adapting operational conditions. So, the present study focused on extracting enzymes from microbial sources to overcome the problems. 260 isolated bacterial strains from different areas of Sylhet, Bangladesh, were screened for their cellulolytic and proteolytic activities using carboxymethylcellulose (CMC) and skim milk agar respectively. Among them 6 cellulolytic and 15 proteolytic bacteria were initially identified. Based on the capability to degrade CMC and skim milk, strain S32 was found to be the most potential among the isolates. Biochemical tests and molecular identification revealed that S32 is a strain of <em>Aeromonas hydrophila</em> and was later named as <em>Aeromonas hydrophila</em> ASM-S32. Maximum cellulase production by the strain <em>A. hydrophila</em> ASM-S32 was obtained after 18 hours (h) of incubation in a fermentation medium with an initial pH of 6.5 at 37°C and that was 4-fold higher as compared to unoptimized conditions. Maximum cellulase activity was observed at 60°C with a pH of 6.5 in presence of Ca<sup>2+</sup> metal ion. In case of protease, optimum enzyme production was observed after 24 h of incubation with an initial pH of 8.5 at 37°C and protease production was increased by 1.2-folds when optimized conditions were used. Maximum protease activity resulted from pH 6.0, at 70°C and in presence of Cu<sup>2+</sup> ion.</p>",
"DOI": "10.5455/jabet.2019.d32",
"views": 740,
"downloads": 84,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure1.jpg",
"caption": "Figure 1. (a) Halos (zones) formed by the cellulolytic bacteria, (b) Zones formed by the proteolytic bacteria.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure2.jpg",
"caption": "Figure 2. Comparison of cellulolytic and proteolytic bacterial activity.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure3.jpg",
"caption": "Figure 3. Representative sequence chromatograms of 16S rDNA of isolate S32.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure4.jpg",
"caption": "Figure 4. Phylogenetic relationship of Aeromonas hydrophila ASM_S32 with other Aeromonas hydrophila strains.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure5.jpg",
"caption": "Figure 5. Optimization of production period for cellulase production. Bars represent means ± standard deviations for three replicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure6.jpg",
"caption": "Figure 6. Optimization of pH for cellulase production. Bars represent means ± standard deviations for three replicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure7.jpg",
"caption": "Figure 7. Optimization of temperature for cellulase production. Bars represent means ± standard deviations for three replicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure8.jpg",
"caption": "Figure 8. Optimization of production period for protease production. Bars represent means ± standard deviations for three replicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure9.jpg",
"caption": "Figure 9. Optimization of pH for protease production. Bars represent means ± standard deviations for three replicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure10.jpg",
"caption": "Figure 10. Optimization of temperature for protease production. Bars represent means ± standard deviations for three replicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure11.jpg",
"caption": "Figure 11. Optimization of pH for cellulase assay. Bars represent means ± standard deviations for three replicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure12.jpg",
"caption": "Figure 12. Optimization of temperature for cellulase assay. Bars represent means ± standard deviations for three replicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure13.jpg",
"caption": "Figure 13. Optimization of temperature for protease assay. Bars represent means ± standard deviations for three replicates.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure14.jpg",
"caption": "Figure 14. Optimization of metal ions for cellulase assay. Bars represent means ± standard deviations for three replicates. *Asterisks with same letter do not differ significantly (P<0.01) whereas asterisks with different letter indicates treatment effects differ significantly (P<0.01) with each other.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/18/29/178-1560250921-Figure15.jpg",
"caption": "Figure 15. Optimization of metal ions for protease assay. Bars represent means ± standard deviations for three replicates. *Asterisks with same letter do not differ significantly (P<0.01) whereas asterisks with different letter indicates treatment effects differ significantly (P<0.01) with each other.",
"featured": false
}
],
"authors": [
{
"id": 235,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Sourav",
"family_name": "Chakraborty",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 68
},
{
"id": 236,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Ziaul Faruque",
"family_name": "Joy",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 68
},
{
"id": 237,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Ashequl",
"family_name": "Haque",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 68
},
{
"id": 238,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Asif",
"family_name": "Iqbal",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 68
},
{
"id": 239,
"affiliation": [
{
"affiliation": "Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "Salma",
"family_name": "Akhter",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 68
},
{
"id": 240,
"affiliation": [
{
"affiliation": "Microbial Biotechnology Division, National Institute of Biotechnology, Savar, Dhaka, Bangladesh"
}
],
"first_name": "Palash Kumar",
"family_name": "Sarker",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 68
},
{
"id": 241,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh"
}
],
"first_name": "S M Abu",
"family_name": "Sayem",
"email": "asayem08@yahoo.com",
"author_order": 7,
"ORCID": "https://orcid.org/0000-0002-9747-0724",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. S M Abu Sayem, Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh",
"article": 68
}
]
},
{
"id": 67,
"slug": "178-1557986892-the-possible-histo-toxicological-impacts-of-long-term-dietary-supplementation-of-soybean-and-canola-oil-on-liver-in-swiss-albino-mice",
"type": "original_article",
"manuscript_id": "178-1557986892",
"published": "2019-09-02",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/54/178-1557986892.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(3): 98-102.",
"academic_editor": "Dr. Md Nabiul Islam, Yamaguchi University, Japan",
"cite_info": "Sharif MA, Haque Z, et al. The possible histo-toxicological impacts of long-term dietary supplementation of Soybean and Canola oil on liver in Swiss albino mice. J Adv Biotechnol Exp Ther. 2019; 2(3): 98-102.",
"title": "The possible histo-toxicological impacts of long-term dietary supplementation of Soybean and Canola oil on liver in Swiss albino mice",
"abstract": "<p>Soybean and Canola oil are widely consumed cooking oils all over the world. Oils and fats are harmful to health so the experiment was conducted to study the possible histo-toxicological impacts of long term dietary supplementation of Soybean and Canola oil on liver in Swiss albino mice. A total of 30 male Swiss albino mice at 6 weeks old were used in the study and inconstantly prorated into 5 equivalent groups as group A was considered as control, group B1 (25 ml so/kg pellet) and B2 (35 ml so/kg pellet) were supplemented with Soybean oil (so) and group C1 (25 ml co/kg pellet) and C2 (35 ml co/kg pellet) supplemented with Canola oil (co) respectively in addition to pellet for 60 days. After completion of study period samples (blood and liver) were collected from the mice of each group and the biochemical, gross and histopathological study was performed. The biochemical study revealed that ALT and AST values were increased significantly in Soybean and Canola oil supplemented groups comparing that of the control group. Gross study revealed that significantly higher liver weight was found in Soybean and Canola oil supplemented groups of mice than the control group. Histopathological study revealed that congested portal vein and dilated bile duct was found in the liver of Soybean oil supplemented groups of mice. There was a congested portal vein was found in the liver of Canola oil supplemented groups of mice. From the present experiment, it could be concluded that Soybean and Canola oil have histo-toxicological effects on the liver of mice.</p>",
"DOI": "10.5455/jabet.2019.d31",
"views": 1004,
"downloads": 147,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/29/178-1557986892-Figure1.jpg",
"caption": "Figure 1. ALT value was increased significantly in both the Soybean (B1, B2) and Canola (C1, C2) oil supplemented groups compared to the control (A) group. Data represented as mean ± SEM, *P<0.05 compared with the control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/29/178-1557986892-Figure2.jpg",
"caption": "Figure 2. AST value was increased significantly in both the Soybean (B1, B2) and Canola (C1, C2) oil supplemented groups compared to the control (A) group. Data represented as mean ± SEM, *P<0.05 compared with the control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/29/178-1557986892-Figure3.jpg",
"caption": "Figure 3. The mean weight of liver was increased significantly in a dose-depended manner in both the Soybean (B1 and B2) and Canola oil (C1 and C2) supplemented groups compared to control (A) group. Data represented as mean ± SEM, *P<0.05 compared with the control.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/41/29/178-1557986892-Figure4.jpg",
"caption": "Figure 4. Histopathological observation of the liver of control and treated groups of mice. The normal morphological appearance was observed in the liver of the control group of mice (A). Marked lymphocytic infiltration (white arrow) was found in the liver of B1 grouped mice. Congested portal vein (black arrow) was found in the liver of B1, C1 and C2 groups of mice. There was also a dilated bile duct (green arrow) that was found in the liver of the B2 group of mice. PV= portal vein, BD= Bile duct, HA= Hepatic artery. Scale bar = 50 μm.",
"featured": false
}
],
"authors": [
{
"id": 232,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Bangladesh Agricultural University"
}
],
"first_name": "Md. Arman",
"family_name": "Sharif",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 67
},
{
"id": 233,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Bangladesh Agricultural University"
}
],
"first_name": "Ziaul",
"family_name": "Haque",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 67
},
{
"id": 234,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Bangladesh Agricultural University"
}
],
"first_name": "M. Rafiqul",
"family_name": "Islam",
"email": "rafiqah77@yahoo.com",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. M. Rafiqul Islam, Professor, Department of Anatomy & Histology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh, e-mail: rafiqah77@yahoo.com",
"article": 67
}
]
}
]
},
{
"id": 8,
"title": "Vol2 Issue2",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2019",
"slug": "2019-vol2-issue2",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 62,
"slug": "178-1555825906-moringa-oleifera-leaves-methanolic-extract-inhibits-angiotensin-converting-enzyme-activity-in-vitro-which-ameliorates-hypertension",
"type": "original_article",
"manuscript_id": "178-1555825906",
"published": "2019-05-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/45/178-1555825906.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(2): 73-77.",
"academic_editor": "Dr. Ataur Rahman, Korea Institute of Science & Technology, South Korea.",
"cite_info": "Aktar S, Das PK, Asha SY, etal. Moringa oleifera leaves methanolic extract inhibits angiotensin converting enzyme activity in vitro which ameliorates hypertension. J Adv Biotechnol Exp Ther. 2019; 2(2): 73-77.",
"title": "Moringa oleifera leaves methanolic extract inhibits angiotensin converting enzyme activity in vitro which ameliorates hypertension",
"abstract": "<p>Angiotensin Converting Enzyme (ACE) regulates blood pressure. ACE converts angiotensin I to angiotensin II which binds with its receptors and through a cascade of reactions constrict blood vesels, consequently results in increased blood pressure, called hypertension. Inhibition of ACE activity is considered as an useful therapeutic target that reduce hypertension. <em>Moringa oleifera</em> leaves have traditionally been used in Ayurvedic medicine for their antihypertensive activity and antihypertensive effect of Moringa leaves was reported on spontaneously hypertensive rats. So, we hypothesize that <em>Moringa oleifera</em> leaves methanolic extract (MOLME) might inhibit ACE activity. Inhibition of ACE activity by MOLME was estimated <em>in vitro</em>. In this study, inhibition of ACE by MOLME was evaluated by spectrophotometric method. MOLME inhibited ACE activity in the substrate hippuryl-L-histidyl-L-leucine (HHL) with an IC50 value of 226.37 μg/ml with a reference compound, captropril (CP), a potent ACE inhibitor with an IC50 value of 0.0289 μM. The mode of ACE inhibition in HHL with or without MOLME revealed that the Vmax (0.0857 and 0.0541 OD/30 min, respectively) was changed and the Km values were 4.671 and 4.41. The results indicate that MOLME acts as a non-competitive inhibitor for ACE. CP was found a competitive inhibitor of ACE. MOLME might be a potential natural inhibitor of ACE which reduces hypertension.</p>",
"DOI": "10.5455/jabet.2019.d28",
"views": 726,
"downloads": 191,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/23/178-1555825906-Figure1.jpg",
"caption": "Figure 1. Inhibition of ACE activity by MOLME. IC50 value of MOLME was calculated to be 226.37±0.021µg/ml. The results are expressed as the percentage of ACE inhibition. The plot represents the mean ± SD from three experiments.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/23/178-1555825906-Figure2.jpg",
"caption": "Figure 2. Inhibition of ACE activity by CP. IC50 value was calculated to be 0.0289 ± 0.011 µM. The results are expressed as the percentage of ACE inhibition. The plot represents the mean ± SD from three experiments.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/23/178-1555825906-Figure3.jpg",
"caption": "Figure 3. Lineweaver-Burk plots for the inhibition of ACE by MOLME (226.37 μg/ml) and CP (0.0289 µM). The plot represents the mean ± SD from three experiments.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/17/23/178-1555825906-Figure4.jpg",
"caption": "Figure 4. Diagram showing the possible effect of MOLME on ACE activity in hypertension.",
"featured": false
}
],
"authors": [
{
"id": 205,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Suraiya",
"family_name": "Aktar",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 62
},
{
"id": 206,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Plabon Kumar",
"family_name": "Das",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 62
},
{
"id": 207,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Saharia Yeasmin",
"family_name": "Ash",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 62
},
{
"id": 208,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Mst. Ayesha",
"family_name": "Siddika",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 62
},
{
"id": 209,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Farhadul",
"family_name": "Islam",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 62
},
{
"id": 210,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Jahan Ara",
"family_name": "Khanam",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 62
},
{
"id": 211,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh."
}
],
"first_name": "Md. Abdur",
"family_name": "Rakib",
"email": "mar@ru.ac.bd",
"author_order": 7,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Md. Abdur Rakib, Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, \r\nBangladesh, Email: mar@ru.ac.bd",
"article": 62
}
]
},
{
"id": 66,
"slug": "178-1555579162-outbreak-of-salmonella-in-poultry-of-bangladesh-and-possible-remedy",
"type": "original_article",
"manuscript_id": "178-1555579162",
"published": "2019-05-22",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1555579162.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(2): 87-97.",
"academic_editor": "Dr. Chu Dinh Toi, University of Oslo, Norway.",
"cite_info": "Hoque MN, Mohiuddin RB, et al. Outbreak of Salmonella in poultry of Bangladesh and possible remedy. J Adv Biotechnol Exp Ther. 2019; 2(2): 87-97.",
"title": "Outbreak of Salmonella in poultry of Bangladesh and possible remedy",
"abstract": "<p>Poultry sector is presently emerged as a great profitable sector in worldwide. The sector’s role in the immense development of the people related this sector. Eggs and hens of layer farms are a major protein source for the people in Bangladesh. Different types of zoonotic diseases prevent the development of the sector. Among the major anxiety related to the development are health issues that scolding not only animal production, but also the community using the food resulting from these animals. One of the most frequently and widely occurring disease is Salmonella. Small-scale commercial farms are predominating here as in Bangladesh where stocks range from several hundreds to thousands and kept in a semi-confined system with a minimum of bio security. In such a system, the birds might be more vulnerable to become exposed to <em>Salmonella</em>. Rainy season is the most suitable for salmonella infection then summer or winter. Prevalence of <em>Salmonella </em>spp. was significantly higher in egg shell compared to egg contents are associated with human illnesses during consumption of contaminated poultry eggs. Different survey notified that, salmonella occurrence in Bangladesh ranges from 20% to above 90% in different locations and seasons. Animals are recognized to be the major reservoir for salmonellae; modern methods of animal husbandry, food production and food handling may encourage the transmission of these organisms from animal products to man. Efforts including critical control point programs in food manufacture are needed to reduce the incidence of <em>Salmonella</em> in food. Consumers-awareness efforts would protect public health from foodborne Salmonellosis.</p>",
"DOI": "10.5455/jabet.2019.d30",
"views": 2353,
"downloads": 193,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/23/178-1555579162-Figure1.jpg",
"caption": "Figure 1. Location wise occurrence of Salmonella in Bangladesh.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/23/178-1555579162-Figure2.jpg",
"caption": "Figure 2. Age dependent occurrence of Salmonella in two regions in Bangladesh.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/23/178-1555579162-Figure3.jpg",
"caption": "Figure 3. Season wise occurrence of Salmonella in Bangladesh.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/28/23/178-1555579162-Figure4.jpg",
"caption": "Figure 4. Factors affecting Salmonellosis in Bangladesh.",
"featured": false
}
],
"authors": [
{
"id": 227,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet, Bangladesh"
}
],
"first_name": "Md. Najmol",
"family_name": "Hoque",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 66
},
{
"id": 228,
"affiliation": [
{
"affiliation": "University of Hohenheim, Germany"
}
],
"first_name": "Rasha Binte",
"family_name": "Mohiuddin",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 66
},
{
"id": 229,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet, Bangladesh"
}
],
"first_name": "Mohammad Mehedi Hasan",
"family_name": "Khan",
"email": "mehedi2001bdbd@gmail.com",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Mohammad Mehedi Hasan Khan, Department of Biochemistry and Chemistry, Sylhet Agricultural University Sylhet, Bangladesh, Email: mehedi2001bdbd@gmail.com",
"article": 66
},
{
"id": 230,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh"
}
],
"first_name": "Afsana",
"family_name": "Hannan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 66
},
{
"id": 231,
"affiliation": [
{
"affiliation": "Department of Animal Production & Management, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Md. Jahangir",
"family_name": "Alam",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 66
}
]
},
{
"id": 63,
"slug": "178-1555480720-influence-of-socio-demographic-factors-on-the-diarrheal-disease-management-approaches-taken-by-two-distinct-communities-of-bangladesh",
"type": "original_article",
"manuscript_id": "178-1555480720",
"published": "2019-05-21",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/22/178-1555480720.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(2): 78-86.",
"academic_editor": "Dr. Masud Parvez, Inje University, South Korea.",
"cite_info": "Khan G, Akter N, Uddin MD, etal. Influence of socio-demographic factors on the diarrheal disease \r\nmanagement approaches taken by two distinct communities of Bangladesh. J Adv Biotechnol Exp Ther. 2019; 2(2): 78-86.",
"title": "Influence of socio-demographic factors on the diarrheal disease management approaches taken by two distinct communities of Bangladesh",
"abstract": "<p>Diarrhea is one of the major determinants of childhood mortality in the world. This study was aimed to provide a demographically representative description on the influence of socio-demographic factors e.g. caretakers’ education, occupation, family income and living standard on the diarrheal disease management approaches taken by the slum dwellers and middle class families of Bangladesh. We have visited 90 middle class families and 120 slum dwellers to obtain information. Children of slum dwellers are more likely to be affected by diarrhea. In both classes, significantly more females were affected by diarrhea than males. This scenario is even more prominent among slum dwellers, where 1.5 times more females were affected by diarrhea than their male counterparts. As a primary approach to manage diarrhea, 63.8% caretakers chose Oral rehydration solution (ORS) whereas 31.5% preferred the salt-molasses fluid. All caretakers knew the use of ORS and antibiotics as a preventive measure against diarrhea. However, this scenario dramatically turned when the caretakers were asked whether they know how to prepare ORS. All the caretakers (100%) in middle class families knew how to prepare ORS in contrast to only 25% caretakers among the slum dwellers. Private sectors specially pharmacies were often the first line of health care in both of these classes during diarrhea. But this is most prevalent among the middle class families (50%), compared to the slum dwellers (35%). Finally, it is apparent that the education, family income, living standard and good food help the middle class families to fight diarrhea more efficiently and scientifically than the slum dwellers.</p>",
"DOI": "10.5455/jabet.2019.d29",
"views": 416,
"downloads": 92,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/23/178-1555480720-Figure1.jpg",
"caption": "Figure 1. Comparison of the age distribution of patients among middle class families and slum dwellers. The occurrence of diarrhea is higher in children and adults with age group between 1-10 and 40-60 years.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/23/178-1555480720-Figure2.jpg",
"caption": "Figure 2. Comparison of the gender distribution of patients among middle class families and slum dwellers. In both types of families, significantly more females were affected by diarrhea than males, which was even more prominent among slum dwellers.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/23/178-1555480720-Figure3.jpg",
"caption": "Figure 3. A combined view of the choice of fluid intake in both of these communities. Most of the caretakers from both families preferred ORS to provide proper hydration for patients during an episode of diarrhea. One-third of the respondents preferred salt-molasses, but very few knew about the rice-based fluids.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/23/178-1555480720-Figure4.jpg",
"caption": "Figure 4. Comparison of the knowledge on how to prepare ORS among the middle class families and slum dwellers. All the caretakers in middle class families knew how to prepare ORS compared to the families living in slums where only one-fourth of them knew how to prepare ORS.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/23/178-1555480720-Figure5.jpg",
"caption": "Figure 5. Comparison of the knowledge about the danger signs of diarrhea among the middle class families and slum dwellers. We investigated whether caretakers recognized danger signs of diarrhea and if they knew when to bring the patients to the health facility. We found that caretakers’ knowledge on the danger sign of diarrhea almost 3 times higher among middle class families compared to the caretakers living in the slum.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/23/178-1555480720-Figure6.jpg",
"caption": "Figure 6. Comparison in the care-seeking behavior among middle class families and slum dweller. We found that most of the caretakers did not consult a licensed health provider and drug shops were often the first line of health care in both classes of the family (50% and 35% cases from middle class families and slum dwellers, respectively).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/23/178-1555480720-Figure7.jpg",
"caption": "Figure 7. Comparison of the use of antibiotics among middle class families and slum dwellers. Here, almost 85% of the patients in middle class families consumed antibiotics compared to only 52% of the patients from slums.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/43/23/178-1555480720-Figure8.jpg",
"caption": "Figure 8. Schematic representation of summary. Our study suggests that knowledge about diarrhea and its management was poor among the caretakers from the slum dwellers compared to the middle class families. Although caretakers from slum dwellers were aware of diarrhea and its home management, their knowledge on vital issues such as complete and correct preparation of ORS, danger signs of dehydration and actual role of oral rehydration fluids during diarrhea was very poor due to the associated socio-demographic factors.",
"featured": false
}
],
"authors": [
{
"id": 212,
"affiliation": [
{
"affiliation": "Department of Biotechnology and Genetic Engineering, Islamic University of Kushtia, Bangladesh"
}
],
"first_name": "Gitika",
"family_name": "Khan",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 63
},
{
"id": 213,
"affiliation": [
{
"affiliation": "Chittagong Medical College, Chittagong, Bangladesh;"
}
],
"first_name": "Nahid",
"family_name": "Akter",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 63
},
{
"id": 214,
"affiliation": [
{
"affiliation": "District Family Planning Office of Chittagong, Ministry of Health and Family Welfare, Bangladesh"
}
],
"first_name": "Md. Dabir",
"family_name": "Uddin",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 63
},
{
"id": 215,
"affiliation": [
{
"affiliation": "Biotechnology Program, Department of Mathematics and Natural Sciences, BRAC University, Dhaka, Bangladesh."
}
],
"first_name": "S M Rakib-Uz",
"family_name": "Zaman",
"email": "rakib.zaman@bracu.ac.bd",
"author_order": 4,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "S M Rakib-Uz-Zaman, Biotechnology Program, Department of Mathematics and Natural Sciences, BRAC University, Dhaka, Bangladesh. Email: rakib.zaman@bracu.ac.bd; Tel.: +880-2-9844051 Ext 4060.",
"article": 63
}
]
},
{
"id": 58,
"slug": "178-1553065003-dhp107-a-novel-oral-paclitaxel-formulation-induces-less-peripheral-neuropathic-pain-and-pain-related-molecular-alteration-than-intravenous-paclitaxel-preparation-in-rat",
"type": "original_article",
"manuscript_id": "178-1553065003",
"published": "2019-05-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/57/178-1553065003.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(2): 55-64.",
"academic_editor": "Dr. Shahed Uddin Ahmed Shazib, University of Ulsan, South Korea.",
"cite_info": "Rahman MM, Son M, Kim H,etal. DHP107, a novel oral paclitaxel formulation induces less peripheral \r\nneuropathic pain and pain-related molecular alteration than intravenous paclitaxel preparation in rat. J Adv Biotechnol Exp Ther. 2019; 2(2): 55-64.",
"title": "DHP107, a novel oral paclitaxel formulation induces less peripheral neuropathic pain and pain-related molecular alteration than intravenous paclitaxel preparation in rat",
"abstract": "<p>Paclitaxel is used in the treatment of cancer especially in breast, stomach and ovarian cancer. However, peripheral neuropathic pain (PNP) induction is the most common devastating side effect of paclitaxel treatment. The objective of this study was to evaluate the PNP related behavioral changes in rats following oral administration of a novel oral paclitaxel formulation DHP107 (Liporaxel) in comparison with another popular intravenous paclitaxel preparation (Taxol). The rats were equally divided in to three groups namely, NC group (normal control): was treated with saline in a matched volume, LPX group (Liporaxel were administered orally) and TAX group (Taxol was administered intravenously). Less pain like behaviors were observed in LPX group in comparison with TAX group evidenced by significant higher level of hot plate paw withdrawal threshold (PWT), von Frey filament PWT and mechanical PWT than TAX group. Reduced lipid peroxidation and elevated antioxidant activities in serum, dorsal root ganglion (DRG) and sciatic nerve (SN) in LPX group than TAX group. In addition, cell apoptosis and expression of pain and neuropathy related proteins activation in LPX group was found lowered and myelin sheath thickness was higher in DRG and SN but not significantly different from the TAX group. Therefore, oral DHP107 could be a promising chemotherapeutic agent due to inducing less PNP.</p>",
"DOI": "10.5455/jabet.2019.d26",
"views": 464,
"downloads": 96,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/55/28/178-1553065003-Figure1.jpg",
"caption": "Figure 1. Effect of oral paclitaxel (Liporaxel®) and intravenous paclitaxel (Taxol®) on body weight and mechanical thresholds in rats. NC group (normal control): was treated with saline in a matched volume; LPX group (Liporaxel® soln were administered orally at 25 mg/kg body weight at 2.5 ml/kg, twice a week) and TAX group (Taxol® was administered intravenously once a week, 10 mg /kg in at 10 ml/kg). HP, hot plate; PW, paw withdrawal. The data are reported as the mean ±SEM (n = 10).*, p < 0.05; **, p< 0.01; ***, p < 0.001, Bonferronipost hoc test following two-way ANOVA versus the NC group. #, p < 0.05; ##, p < 0.01 and ###, p < 0.001, Bonferronipost hoc test following two-way ANOVA versus the LPX group.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/55/28/178-1553065003-Figure2.jpg",
"caption": "Figure 2. Effect of oral paclitaxel (Liporaxel®) and intravenous paclitaxel (Taxol®) on morphometric changes of dorsal root ganglion and sciatic nerve. NC group (normal control): was treated with saline in a matched volume; LPX group (Liporaxel® soln were administered orally at 25 mg/kg body weight at 2.5 ml/kg, twice a week) and TAX group (Taxol® was administered intravenously once a week, 10 mg /kg in at 10 ml/kg). DRG, dorsal root ganglion; SN, sciatic nerve. The data are reported as the mean ±SEM (n = 10).*, p < 0.05 Bonferronipost hoc test following one-way ANOVA versus the NC group. #, p < 0.05 Bonferronipost hoc test following one-way ANOVA versus the LPX group but no significance change was observed.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/55/28/178-1553065003-Figure3.jpg",
"caption": "Figure 3. Effect of oral paclitaxel (Liporaxel®) and intravenous paclitaxel (Taxol®) on the expression of GFAP, Iba1, Nitro and MPZ in the dorsal root ganglion and sciatic nerve in rat. NC group (normal control): was treated with saline in a matched volume; LPX group (Liporaxel® soln were administered orally at 25 mg/kg body weight at 2.5 ml/kg, twice a week) and TAX group (Taxol® was administered intravenously once a week, 10 mg /kg in at 10 ml/kg). GFAP, glial fibrillary acidic protein; Iba-1, ionized calcium-binding adapter molecule-1; Nitro, nitrotyrosine and MPZ, Myelin protein zero. The data are reported as the mean ±SEM (n = 10).*, p < 0.05 Bonferronipost hoc test following one-way ANOVA versus the NC group. #, p < 0.05 Bonferronipost hoc test following one-way ANOVA versus the LPX group but no significance change was observed.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/55/28/178-1553065003-Figure4.jpg",
"caption": "Figure 4. Effect of oral paclitaxel (Liporaxel®) and intravenous paclitaxel (Taxol®) on the expression of GFAP, Iba1, Nitro and MPZ in the sciatic nerve in rat. NC group (normal control): was treated with saline in a matched volume; LPX group (Liporaxel® soln were administered orally at 25 mg/kg body weight at 2.5 ml/kg, twice a week) and TAX group (Taxol® was administered intravenously once a week, 10 mg /kg in at 10 ml/kg). GFAP, glial fibrillary acidic protein; Iba-1, ionized calcium-binding adapter molecule-1; Nitro, nitrotyrosine and MPZ, Myelin protein zero. The data are reported as the mean ±SEM (n = 10).*, p < 0.05 Bonferronipost hoc test following one-way ANOVA versus the NC group. #, p < 0.05 Bonferronipost hoc test following one-way ANOVA versus the LPX group but no significance change was observed.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/55/28/178-1553065003-Figure5.jpg",
"caption": "Figure 5. Effect of oral paclitaxel (Liporaxel®) and intravenous paclitaxel (Taxol®) on the apoptosis in the dorsal root ganglion and sciatic nerve in rat. NC group (normal control): was treated with saline in a matched volume; LPX group (Liporaxel® soln were administered orally at 25 mg/kg body weight at 2.5 ml/kg, twice a week) and TAX group (Taxol® was administered intravenously once a week, 10 mg /kg in at 10 ml/kg). DRG, dorsal root ganglion; SN, sciatic nerve. (Scale bar in case of DRG = 200 μm and incase of SN 1000 μm). The data are reported as the mean ±SEM (n = 10).*, p < 0.05 Bonferronipost hoc test following one-way ANOVA versus the NC group. #, p < 0.05 Bonferronipost hoc test following one-way ANOVA versus the LPX group but no significance change was observed.",
"featured": false
}
],
"authors": [
{
"id": 180,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd. Research Center, 189 Donggureung-Ro, Guri-Si, Gyeonggi-Do, Republic of Korea"
}
],
"first_name": "Md. Mahbubur",
"family_name": "Rahman",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 58
},
{
"id": 181,
"affiliation": [
{
"affiliation": "Daehwa Pharmaceutical Co. Ltd, C-9th, Korea BioPark, 700, Daew angpangyo-Ro, Bundang-Gu, Seongnam-Si, geongi-Do, Republic of Korea"
}
],
"first_name": "Minhee",
"family_name": "Son",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 58
},
{
"id": 182,
"affiliation": [
{
"affiliation": "Daehwa Pharmaceutical Co. Ltd, C-9th, Korea BioPark, 700, Daew angpangyo-Ro, Bundang-Gu, Seongnam-Si, geongi-Do, Republic of Korea"
}
],
"first_name": "Hyun",
"family_name": "Kim",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 58
},
{
"id": 183,
"affiliation": [
{
"affiliation": "Daehwa Pharmaceutical Co. Ltd, C-9th, Korea BioPark, 700, Daew angpangyo-Ro, Bundang-Gu, Seongnam-Si, geongi-Do, Republic of Korea"
}
],
"first_name": "In-Hyun",
"family_name": "Lee",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 58
},
{
"id": 184,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd. Research Center, 189 Donggureung-Ro, Guri-Si, Gyeonggi-Do, Republic of Korea"
}
],
"first_name": "Ha-young",
"family_name": "Jeon",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 58
},
{
"id": 185,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd. Research Center, 189 Donggureung-Ro, Guri-Si, Gyeonggi-Do, Republic of Korea"
}
],
"first_name": "Myung-Jin",
"family_name": "Kim",
"email": null,
"author_order": 6,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 58
},
{
"id": 186,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd. Research Center, 189 Donggureung-Ro, Guri-Si, Gyeonggi-Do, Republic of Korea"
}
],
"first_name": "Hansol",
"family_name": "Kwon",
"email": null,
"author_order": 7,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 58
},
{
"id": 187,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd. Research Center, 189 Donggureung-Ro, Guri-Si, Gyeonggi-Do, Republic of Korea"
}
],
"first_name": "Sung-Jin",
"family_name": "Park",
"email": null,
"author_order": 8,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 58
},
{
"id": 188,
"affiliation": [
{
"affiliation": "Daehwa Pharmaceutical Co. Ltd, C-9th, Korea BioPark, 700, Daew angpangyo-Ro, Bundang-Gu, Seongnam-Si, geongi-Do, Republic of Korea"
}
],
"first_name": "Junhee",
"family_name": "Jang",
"email": "jhjang@dhpharm.co.kr",
"author_order": 9,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Daehwa Pharmaceutical Co. Ltd, C-9th, Korea BioPark, 700, Daew angpangyo-Ro, Bundang-Gu, Seongnam-Si, geongi-Do, Republic of Korea. e-mail: jhjang@dhpharm.co.kr",
"article": 58
},
{
"id": 189,
"affiliation": [
{
"affiliation": "KNOTUS Co., Ltd. Research Center, 189 Donggureung-Ro, Guri-Si, Gyeonggi-Do, Republic of Korea"
}
],
"first_name": "Sokho",
"family_name": "Kim",
"email": "skim@knotus.co.kr",
"author_order": 10,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "KNOTUS Co., Ltd. Research Center, 189 Donggureung-Ro, Guri-Si, Gyeonggi-Do, Republic of Korea. , E-mail: skim@knotus.co.kr",
"article": 58
}
]
},
{
"id": 61,
"slug": "178-1554885136-in-vitro-plant-regeneration-of-wild-eggplant-solanum-sisymbriifolium-to-produce-large-number-of-rootstocks-for-tomato-grafting",
"type": "original_article",
"manuscript_id": "178-1554885136",
"published": "2019-05-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/13/178-1554885136.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(2): 65-72.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research, Dhaka-1230, Bangladesh.",
"cite_info": "Deb G, Sultana S, Bhuiyan MSU, etal. In vitro plant regeneration of wild eggplant (Solanum sisymbriifolium) to produce large number of rootstocks for tomato grafting 2019 Volume2 Issue2. J Adv Biotechnol Exp Ther. 2019; 2(2): 65-72.",
"title": "In vitro plant regeneration of wild eggplant (Solanum sisymbriifolium) to produce large number of rootstocks for tomato grafting",
"abstract": "<p>The experiment was conducted to develop a suitable protocol for high frequency plant regeneration of wild eggplant (<em>Solanum sisymbriifolium</em>) in order to produce a large number of rootstocks for tomato grafting for the management of wilt disease. To obtain <em>in vitro</em> seedlings of<em> S. sisymbriifolium</em>, seeds were treated with various concentrations of GA<sub>3</sub> (Gibberellic acid) prior to place them in germination media (½ strength Murashige and Skoog) and 750 mg/L GA<sub>3</sub> was found as a suitable concentration resulting the highest (76.67%) germination rate. Various factors namely combination of plant growth regulators, explant types and explant age were investigated for development of an efficient plant regeneration system of <em>S. sisymbriifolium</em>. Cotyledon and hypocotyl explants of<em> S. sisymbriifolium</em> were cultured on Murashige and Skoog medium supplemented with various concentrations of BA (6-Benzylaminopurine), NAA (α-Naphthalene acetic acid) and 2,4-D (2,4-Dichlorophenoxy acetic acid), to determine suitable medium for callus and shoot initiation. Fourteen days old cotyledon explants were found more responsive than that of hypocotyl, both in callus and shoot induction. The highest callus initiation (100%) and shoot regeneration (73.33%) were observed in MS media supplemented with 0.5 mg/L NAA + 1.0 mg/L BA and 0.2 mg/L NAA + 3.0 mg/L BA, respectively. MS medium supplemented with 0.1 mg/L NAA showed the highest frequency (86.67%) of rooting. The regenerated plantlets were acclimatized in pot soil and eventually used as rootstock for tomato (<em>Solanum lycopersicum</em> cv. BARI hybrid 4) grafting. The grafted plants showed no wilt disease in field condition until maturity.</p>",
"DOI": "10.5455/jabet.2019.d27",
"views": 1032,
"downloads": 100,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/25/178-1554885136-Figure1.jpg",
"caption": "Figure 1. The regeneration process of S. sisymbriifolium. (a) 14 days old seedlings grown in half strength MS media, (b) hypocotyl explants on callus induction medium (MS + 0.5 mg/L NAA + 1 mg/L BA) at first day of culture, (c) cotyledon explants on callus induction medium at first day of culture, (d) 14-d-old callus obtained from cotyledon explants in callus induction medium, (e) shoot initiation in shoot induction medium (MS + 0.2 mg/L NAA + 3 mg/L BA), (f) initiation of roots in MS + 0.1 mg/L NAA medium, (g) acclimatized plant in soil, (h) Grafted tomato ( S. lycopersicum cv. BARI hybrid tomato 4) plant where S. sysimbriifolium was used as rootstock, (i) flowered grafted-tomato plant in natural environment. Scale bars represent 5mm (b, c, d), 1 cm (a, e, f, g), 2 cm (h), 5 cm (i).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/25/178-1554885136-Figure2.jpg",
"caption": "Figure 2. Frequency of callus initiation from 14 days old cotyledon and hypocotyl explants of S. sisymbriifolium on MS media supplemented with various concentrations of BA, NAA and 2,4-D. Data consist of three replications and 10 explants were used for each replication. Bars represent SD of means. Values with different letters are significantly different at P value = .05 (DMRT).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/25/178-1554885136-Figure3.jpg",
"caption": "Figure 3. Frequency of shoot regeneration from callus (14 days old) originated from cotyledon and hypocotyl explants on shoot regeneration media after 21 days of culture. Data consist of three replications and 5 explants were used for each replication. Bars represent SD of means. Values with different letters are significantly different at P value = .05 (DMRT).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/25/178-1554885136-Figure4.jpg",
"caption": "Figure 4. Effect of explant age on shoot regeneration from cotyledon explants of S. sisymbriifolium. Data consist of three replications and 5 explants were used for each replication. Bars represent SD of means. Values with different letters are significantly different at P value = .05 (DMRT).",
"featured": false
}
],
"authors": [
{
"id": 200,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Goutam",
"family_name": "Deb",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 61
},
{
"id": 201,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Sayeda",
"family_name": "Sultana",
"email": "lopacnu@yahoo.com",
"author_order": 2,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sayeda Sultana, Assistant Professor, Department of Genetics and Plant Breeding, Sylhet Agricultural University, \r\nSylhet-3100, Bangladesh, Email: lopacnu@yahoo.com, Tel.: +880-1732036675.",
"article": 61
},
{
"id": 202,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Mohammed Shafi Ullah",
"family_name": "Bhuiyan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 61
},
{
"id": 203,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Kishore Kumar",
"family_name": "Sarker",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 61
},
{
"id": 204,
"affiliation": [
{
"affiliation": "Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet-3100, Bangladesh"
}
],
"first_name": "Ashrafi Sultana",
"family_name": "Papry",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 61
}
]
},
{
"id": 57,
"slug": "178-1552415618-mutation-detection-sensitivity-of-high-resolution-melting-in-clinical-samples-a-comparative-study-between-formamide-and-dimethyl-sulfoxide",
"type": "original_article",
"manuscript_id": "178-1552415618",
"published": "2019-05-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/47/178-1552415618.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(2): 51-54. (accepted).",
"academic_editor": "Dr. Md Mahmodul Hasan, Erciyes University, Turkey.",
"cite_info": "Roy D, Hasa MM, Haque A. Mutation detection sensitivity of high resolution melting in clinical samples: a comparative study between formamide and dimethyl sulfoxide. J Adv Biotechnol Exp Ther. 2019; 2(2): 51-54. (accepted).",
"title": "Mutation detection sensitivity of high resolution melting in clinical samples: a comparative study between formamide and dimethyl sulfoxide",
"abstract": "<p>High-resolution melting (HRM) is one of the widely used methods for mutation detection. Sometimes, mutations which are rare but clinically important may not be detected. Thus, increasing sensitivity of HRM based mutation detection method is essential. This study was particularly aimed to establish HRM based mutation detection method with improved sensitivity. However, we had taken an attempt to detect the mutations in transcription factor 7-like 2 (<em>TCF7L2</em>) gene by HRM analysis but we experienced poor sensitivity in mutation detection. Hence, we tried to increase the sensitivity of HRM by adding formamide and dimethyl sulfoxide (DMSO). We used the final concentration of formamide and DMSO at 0.2% and 7%, respectively and found that formamide had better efficacy in increasing HRM sensitivity than DMSO.</p>",
"DOI": "10.5455/jabet.2019.d25",
"views": 620,
"downloads": 127,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/53/25/178-1552415618-Figure1.jpg",
"caption": "Figure 1. Gel photograph after (A) gradient PCR at 55-62°C, and (B) normal PCR at only 60°C. In case of gradient PCR, lanes 1, 2, 3, 4, 5, 6, 7, and 8 represent PCR products of corresponding annealing temperature of 55, 56, 57, 58, 59, 60, 61, and 62°C respectively, where lane 6 (annealing temperature is 60°C) showed the brightest band. In case of normal PCR, lane 1 represents DNA ladder (1 kb plus), whereas lane 2, 3, 4, and 5 represent four different PCR products (~160 bp) at only 60°C annealing temperature.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/53/25/178-1552415618-Figure2.jpg",
"caption": "Figure 2. Normalized melt curve for (A) without both of formamide and DMSO, (B) without and with formamide, and (C) with both of formamide and DMSO. X axis represents temperature (°C) and Y axis represents normalized fluorescence.",
"featured": false
}
],
"authors": [
{
"id": 177,
"affiliation": [
{
"affiliation": "Molecular Pathology Laboratory, Institute of Biological Sciences (IBSc), University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Dipa",
"family_name": "Roy",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 57
},
{
"id": 178,
"affiliation": [
{
"affiliation": "Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Md. Mahmudul",
"family_name": "Hasan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 57
},
{
"id": 179,
"affiliation": [
{
"affiliation": "Molecular Pathology Laboratory, Institute of Biological Sciences (IBSc), University of Rajshahi, Rajshahi-6205, Bangladesh"
}
],
"first_name": "Ariful",
"family_name": "Haque",
"email": "haque@ru.ac.bd",
"author_order": 3,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Ariful Haque, Molecular Pathology Laboratory, Institute of Biological Sciences (IBSc), University of Rajshahi, Rajshahi\u00026205, Bangladesh, e-mail: haque@ru.ac.bd",
"article": 57
}
]
},
{
"id": 51,
"slug": "178-1546820446-herbal-contraceptive-effect-of-abrus-precatorius-ricinus-communis-and-syzygium-aromaticum-on-anatomy-of-the-testis-of-male-swiss-albino-mice",
"type": "original_article",
"manuscript_id": "178-1546820446",
"published": "2019-05-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/21/178-1546820446.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(2): 36-43.",
"academic_editor": "Dr. Md Nabiul Islam, Yamaguchi University, Japan.",
"cite_info": "Bhakta S, Awal A, Das SK. Herbal contraceptive effect of Abrus precatorius, Ricinus communis, and Syzygium aromaticum on anatomy of the testis of male Swiss albino mice. J Adv Biotechnol Exp Ther. 2019; 2(2): 36-43.",
"title": "Herbal contraceptive effect of Abrus precatorius, Ricinus communis, and Syzygium aromaticum on anatomy of the testis of male Swiss albino mice",
"abstract": "<p>The present research was designed to investigate in vivo herbal contraceptive effects on the anatomy of the testis in male Swiss albino mice (<em>Mus musculus</em>) and to observe that whether the herbal extracts depart any baleful effects on the genitalia or not. Swiss albino mice at age of 30 days were divided into two groups: control and treated group. From day 60, the treated group was administered orally with an aqueous extract (4.4 mg/kg body weight) prepared from the herbal plants. At day 97 (after treatment for 6 weeks) both the control and treated mice were sacrificed. Testes were collected for anatomical (gross and histomorphological) studies. Hematoxylin and Eosin staining was performed for the histo-morphological studies. In the present study, the gross anatomy of the testis were significantly (p<0.05) reduced and pale in color in treated mice in comparison to the control group. Histologically, number of seminiferous tubules, sertoli & leydig cells and amount of spermatozoa within the lumen of seminiferous tubules were decreased. Derangement of the seminiferous tubules along with the presence of a thick fibrous layer and vacuoles were found within and between the seminiferous tubules in the treated group. The aforesaid changes with a good contraception rate (75%) and devoid of any baleful effects even on the female were found, where the contraceptive pills are known to have many side effects. Considering above all, the mixture of plants seems to be effectively worked on the male gonad (testis) suggesting that the herbal extracts might be future use-worthy.</p>",
"DOI": "10.5455/jabet.2019.d23",
"views": 1467,
"downloads": 139,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/28/178-1546820446-Figure1.jpg",
"caption": "Figure 1. Gross anatomy (weight-A, length-B and diameter-C) of the testis of control (C) and treated (T) showing the reduction of size.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/02/28/178-1546820446-Figure2.jpg",
"caption": "Figure 2. Histological architecture of testis showing the normal testis of the control group (A, C E, G) and (B) reduced number of seminiferous tubules (arrow) and spermatozoa (arrow head) in treated testis of the group T;(H&E, X 10); (D) derangement of the seminiferous tubules (arrow) in the treated group T (H&E, X 10); (F) presence of a fibrin thickening (arrow) & vacuoles (fat deposition) (arrow head) in the testis of the treated group (arrow) (H&E, X 40) and (H) reduction of the amount of the sertoli (arrow) and leydig cells (arrow head) in the treated group, but less in the group C (H&E, X 40).",
"featured": false
}
],
"authors": [
{
"id": 149,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Sonali",
"family_name": "Bhakta",
"email": "sonali.dvm@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Sonali Bhakta, Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural \r\nUniversity, Mymensingh-2202, Bangladesh, Email: sonali.dvm@gmail.com",
"article": 51
},
{
"id": 150,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Abdul",
"family_name": "Awal",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 51
},
{
"id": 151,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Shonkor Kumar",
"family_name": "Das",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 51
}
]
},
{
"id": 56,
"slug": "178-1550814536-evaluation-of-density-metric-grading-of-agarwood-antioxidant-potentiality-in-agar-oil-and-prevalence-of-unknown-bacteria-in-agarwood-soaking-water",
"type": "original_article",
"manuscript_id": "178-1550814536",
"published": "2019-05-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/24/178-1550814536.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(2): 44-50.",
"academic_editor": "Dr. Md. Abdul Hannan, Dongguk University, South Korea.",
"cite_info": "Ahmed J, Khan MMH, Kashem MA, etal. Evaluation of density metric grading of agarwood, antioxidant potentiality in agar oil, and prevalence of unknown bacteria in agarwood soaking water. J Adv Biotechnol Exp Ther. 2019; 2(2): 44-50.",
"title": "Evaluation of density metric grading of agarwood, antioxidant potentiality in agar oil, and prevalence of unknown bacteria in agarwood soaking water",
"abstract": "<p>The experiments were conducted for grading unknown agarwood, to analyze the total phenolics, total flavonoids, antioxidant status of agarwood and its crude oil and also to identify the existing bacteria that assist in the fermentation of agarwood during soaking period. Grades of unknown agarwoods were determined based on density metric method and cross checked for ether extract. Total phenolics of agar wood and oils were analyzed through Folin-Ciocalteu method and total flavonoid contents of agar oils were estimated using Aluminium chloride colorimetric method. For the determination of antioxidant status of agarwood oil, the inhibitory effects of oil extracts on DPPH radical was measured. To identify the bacterial genus, several biochemical tests were conducted. Results showed that insect infested agarwood was classified as Grade-1 agarwood (Calculated density was 0.641 g/cm<sup>3</sup>) and contained the highest amount ether extract of 18.90 ± 0.60%, total phenolic content of 3.5 ± 0.06 (mg GAE/g) and flavonoid content of 7.82 ± 0.23 (µg QE/ml). IC<sub>50</sub> values obtained by DPPH activity for <em>Aquillaria malaccensis</em> oil extract was found to be 0.904 µg/ml. <em>Bacillus</em> spp. was recognized as the mostly prevalent bacteria responsible for fermentation of agarwood which exhibit special role in increasing the yield of agar oil. If agarwood and agar oil can be graded properly high market value will be obtained by exporting them.</p>",
"DOI": "10.5455/jabet.2019.d24",
"views": 909,
"downloads": 155,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/28/178-1550814536-Figure1.jpg",
"caption": "Figure 1. Determination of density of agarwood through mass volume relationship.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/28/178-1550814536-Figure2.jpg",
"caption": "Figure 2. Overall prevalence of bacterial isolates in agarwood soaking water.",
"featured": false
}
],
"authors": [
{
"id": 172,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet-3100"
}
],
"first_name": "Jamil",
"family_name": "Ahmed",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 56
},
{
"id": 173,
"affiliation": [
{
"affiliation": "Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet-3100"
}
],
"first_name": "Mohammad Mehedi Hasan",
"family_name": "Khan",
"email": "mehedi2001bdbd@gmail.com",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-4814-3065",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Mehedi Hasan Khan, Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet-3100, E-mail: mehedi2001bdbd@gmail.com",
"article": 56
},
{
"id": 174,
"affiliation": [
{
"affiliation": "Department of Soil Science, Sylhet Agricultural \r\nUniversity, Sylhet-3100"
}
],
"first_name": "Md. Abul",
"family_name": "Kashem",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 56
},
{
"id": 175,
"affiliation": [
{
"affiliation": "Department of Plant and Environmental Biotechnology, Sylhet Agricultural University, Sylhet-3100"
}
],
"first_name": "Sheikh Rashel",
"family_name": "Ahmed",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 56
},
{
"id": 176,
"affiliation": [
{
"affiliation": "Department of Forest Chemistry, Bangladesh Forest Research Institute, Chittagong, Bangladesh"
}
],
"first_name": "Mohammad Jakir",
"family_name": "Hossain",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 56
}
]
}
]
},
{
"id": 6,
"title": "Vol2 Issue1",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2019",
"slug": "2019-vol2-issue1",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 50,
"slug": "178-1545186273-prophylactic-effects-of-vitamin-e-and-coriander-coriandrum-sativum-extract-on-lead-induced-testicular-damage-in-swiss-albino-mice",
"type": "original_article",
"manuscript_id": "178-1545186273",
"published": "2019-01-13",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/41/178-1545186273.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(1) : 31-35.",
"academic_editor": "Dr. Hasan-Al-Faruque, Daegu Gyeonbuk Institute of Science and Technology, South Korea.",
"cite_info": "Islam MR, Jahid MA, et al. Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice. J Adv Biotechnol Exp Ther. 2019; 2(1) : 31-35.",
"title": "Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice",
"abstract": "<p>Lead toxicity is the vital issue in the developing countries that causes serious health hazards in animals and humans. Prophylactic effects of vitamin E and coriander (<em>Coriandrum sativum</em>) extract on lead-induced testicular damage in Swiss albino mice were investigated in the present study by gross and histological studies in five groups (control, lead intoxicated, vitamin E-treated, coriander-treated, vitamin E and coriander-treated). Treatment was done for 42 days. In the present study, weight and length of left and right testes were significantly (p<0.05) reduced in lead-intoxicated mice in comparison to the control group. Lead acetate was found to cause separation of primary spermatocyte from spermatogonia, morphological changes of seminiferous tubule and irregular arrangement of spermatogenic cells in the seminiferous tubules of testis. Vitamin E and coriander extract were found effective in the treatment of lead intoxicated mice and testes histology were found normal. Combined antioxidative action of vitamin E and corriander extract was also found effective in the treatment of lead intoxicated mice. The present investigation may serve as baseline data about adverse effects of lead toxicity and efficacy of vitamin E and coriander extract against lead toxicity. Further research need to be done to isolate and purify the active principle involved in the antioxidant activity of this plant.</p>",
"DOI": "10.5455/jabet.2018.d22",
"views": 937,
"downloads": 126,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/23/178-1545186273-Fig1.jpg",
"caption": "Figure 1. Gross observation of left testis in mice (only left testes of different groups are presented here because there was no gross difference between left and right testes.). Normal appearance of testis in control group (A), vitamin E treated group (C), coriander extract treated group (D) and vitamin E and coriander extract treated group (E). Testis was found in reduced size in lead intoxicated group (B). *(p<0.05).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/46/23/178-1545186273-Fig2.jpg",
"caption": "Figure 2. Histological observation of testis in mice (H&E). Histological observation of left testis is presented here because there was no histological differentiation between left and right testes. Normal appearance of seminiferous tubules in control group. Spermatogenic cells were present in normal pattern (A). Primary spermatocytes were found separated from spermatogonia (black arrowhead) in lead intoxicated group (B). In addition, morphological changes of seminiferous tubules (double arrow head) and irregular arrangement of spermatogenic cells (white arrow) were observed in this group (C). Appearance of seminiferous tubules was found normal in vitamin E treated group (D), coriander extract treated group (E) and vitamin E and coriander extract treated group (F). Spermatogenic cells were present in normal pattern in these groups. Scale bar = 100 µm.",
"featured": false
}
],
"authors": [
{
"id": 145,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Bangladesh Agricultural University"
}
],
"first_name": "Mohammad Rafiqul",
"family_name": "Islam",
"email": "rafiqah77@yahoo.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-8852-265X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Mohammad Rafiqul Islam, Department of Anatomy & Histology, Bangladesh Agricultural University,\r\nMymensingh-2202, Bangladesh, email: rafiqah77@yahoo.com",
"article": 50
},
{
"id": 146,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Bangladesh Agricultural University"
}
],
"first_name": "Md. Anwar",
"family_name": "Jahid",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 50
},
{
"id": 147,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Bangladesh Agricultural University"
}
],
"first_name": "Md Zahirul Islam",
"family_name": "Khan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 50
},
{
"id": 148,
"affiliation": [
{
"affiliation": "Division of Neuroanatomy, Yamaguchi University School of Medicine, Ube, Japan"
}
],
"first_name": "Md. Nabiu",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 50
}
]
},
{
"id": 48,
"slug": "178-1541252802-a-brief-review-on-japanese-encephalitis-in-swine-with-its-diagnostic-strategies",
"type": "review_article",
"manuscript_id": "178-1541252802",
"published": "2019-01-12",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/52/178-1541252802.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(1) : 23-30.",
"academic_editor": "Dr. Md Jamal Uddin, Ewha Womans University, South Korea.",
"cite_info": "Ghana M, Das PK. A brief review on Japanese Encephalitis in Swine with its diagnostic \r\nstrategies. J Adv Biotechnol Exp Ther. 2019; 2(1) : 23-30.",
"title": "A brief review on Japanese Encephalitis in Swine with its diagnostic strategies",
"abstract": "<p>Japanese encephalitis is a viral disease affecting humans, mostly the children and is a major concern to public health. The single stranded flavivirus is mainly transmitted by mosquitoes of the Culex genus and causes neurological disorders with high fatality. The virus is well amplified in animals like pigs (swine) that act as a host. Close proximity of the domesticated pigs to human settlements increases the risk of human infection. The animal to human zoonoses of this antigen is occurred through the mosquitoes bite. Recent developments have led to discovery of various vaccines for the treatment of the disease. This review discusses various aspects of the Japanese encephalitis disease with their transmission, pathogenesis and diagnostic strategies. Moreover it highlights the importance of prevention of transmission of the disease over its cure by proposing a hypothetical kit design model to detect presence of the Japanese encephalitis antigen in the swine populations. This detection process may help to detect infected animals and thus help to keep such animals away from the human settlements so as to prevent transmission as well as outbreak of the disease. This may further help to create a disease free environment, thus saving many lives.</p>",
"DOI": "10.5455/jabet.2018.d21",
"views": 1315,
"downloads": 101,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/23/178-1541252802-Fig1.jpg",
"caption": "Figure 1. Reported cases of Japanese Encephalitis and the deaths involved therein.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/23/178-1541252802-Fig2.jpg",
"caption": "Figure 2. Epidemiological patterns of Japanese encephalitis.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/23/178-1541252802-Fig3.jpg",
"caption": "Figure 3. Transmission cycle of Japanese encephalitis virus.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/25/23/178-1541252802-Fig4.jpg",
"caption": "Figure 4. Hypothetical Kit design using citrate stabilized gold nanoparticles for detection of Japanese encephalitis in swine.",
"featured": false
}
],
"authors": [
{
"id": 136,
"affiliation": [
{
"affiliation": "Centre for Biotechnology, Siksha ‘O’ Anusandhan , Bhubaneswar, Odisha – 751003, India."
}
],
"first_name": "Madhusmita",
"family_name": "Ghana",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 48
},
{
"id": 137,
"affiliation": [
{
"affiliation": "Centre for Biotechnology, Siksha ‘O’ Anusandhan , Bhubaneswar, Odisha – 751003, India."
}
],
"first_name": "Pratyush Kumar",
"family_name": "Das",
"email": "pratyushdas@soa.ac.in",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0002-7252-380X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Pratyush Kumar Das, Centre for Biotechnology, Siksha ‘O’ Anusandhan, Bhubaneswar, Odisha – 751003, India. \r\nEmail: pratyushdas@soa.ac.in",
"article": 48
}
]
},
{
"id": 47,
"slug": "178-1541767950-a-reliable-and-easy-method-to-isolate-a-pure-population-of-bovine-granulosa-cells-from-slaughterhouse-ovaries-for-in-vitro-studies",
"type": "original_article",
"manuscript_id": "178-1541767950",
"published": "2019-01-10",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/51/178-1541767950.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(1) : 17-22.",
"academic_editor": "Dr. Md Nabiul Islam, Yamaguchi University, Japan.",
"cite_info": "Sohel MMH. A reliable and easy method to isolate a pure population of bovine \r\ngranulosa cells from slaughterhouse ovaries for in vitro studies. J Adv Biotechnol Exp Ther. 2019; 2(1) : 17-22.",
"title": "A reliable and easy method to isolate a pure population of bovine granulosa cells from slaughterhouse ovaries for in vitro studies",
"abstract": "<p>In a growing follicle, granulosa cells (GCs) are the major cellular component and perform several important functions. GCs are an excellent choice for in vitro experimentation to understand their functions in vivo. However, the isolation of a pure fraction of GCs from slaughterhouse ovaries remains a challenge. The current study describes a simpler yet reliable method for isolating GCs from slaughterhouse ovaries of domestic animals. For this, bovine ovaries were collected from a nearby slaughterhouse and GCs were collected by aspiration method, one fraction of fresh GCs was snap frozen and stored at -80°C for further analysis and another fraction was used in culture purpose. The purity of GCs was determined by quantifying follicle stimulating hormone receptor (FSHR) and CYP17A1 transcripts and analyzing the cultured cells under a microscope. The viability of GCs ranged from 66 – 35 % depending on the time and media used in transportation-processing. The results showed that FSHR gene was highly expressed as evidenced by the presence of a strong band while CYP17A1 is completely absent in the GCs isolated using the current method. In addition, the characteristics of the cultured cells further confirm the purity of isolated GCs. The current method isolates a pure population of GCs from slaughterhouse ovary without any contamination of thecal cells.</p>",
"DOI": "10.5455/jabet.2018.d20",
"views": 1378,
"downloads": 127,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/11/178-1541767950-Figure1.jpg",
"caption": "Figure 1. The whole workflow.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/11/178-1541767950-Figure2.jpg",
"caption": "Figure 2. The viability of GCs using trypan blue exclusion test.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/11/178-1541767950-Figure3.jpg",
"caption": "Figure 3. The shape and morphology of GCs in culture were evaluated using a phase-contrast microscopy.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/08/11/178-1541767950-Figure4.jpg",
"caption": "Figure 4. FSHR and CYP17A1 mRNA expression in freshly isolated GCs using PCR analysis.",
"featured": false
}
],
"authors": [
{
"id": 135,
"affiliation": [
{
"affiliation": "Genome and Stem Cell Centre, Erciyes University, Kayseri 38039, Turkey."
},
{
"affiliation": "Department of Animal Science, Faculty of Agriculture, Erciyes University, Kayseri 38039, Turkey."
}
],
"first_name": "Md Mahmodul Hasan",
"family_name": "Sohel",
"email": "sohel@erciyes.edu.tr",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Md Mahmodul Hasan Sohel, Department of Animal Science, Faculty of Agriculture, Erciyes University, Kayseri \r\n38039, Turkey. Email: sohel@erciyes.edu.tr",
"article": 47
}
]
},
{
"id": 45,
"slug": "178-1542113052-spectrochemical-characterization-of-vero-cell-line-against-ppr-virus-infection",
"type": "original_article",
"manuscript_id": "178-1542113052",
"published": "2019-01-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/24/178-1542113052.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(1) : 10-16.",
"academic_editor": "Dr. Md Masud Parvez, Inje University, South Korea",
"cite_info": "Rahman MM, Roy KJ, Aktar MK, etal. Spectrochemical characterization of Vero cell line against PPR virus infection. J Adv Biotechnol Exp Ther. 2019; 2(1) : 10-16.",
"title": "Spectrochemical characterization of Vero cell line against PPR virus infection",
"abstract": "<p>An in vitro virus infectivity assay based on the newly introduced spectrochemical method was performed using <em>Peste des Petits</em> Ruminants (PPR) virus infected Vero cell line as a model infection. Herein, the mitochondrial reductase enzyme activity was monitored using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent as a substrate which was reduced to a insoluble formazan products. The purple to darker color was achieved upon hydrolysis of the insoluble formazan with DMSO solution. The absorbance (OD values) of colored solution was measured by spectrophotometer at a wave length of 570nm. The infectivity/viability data were achieved from the OD values of different doses of virus infected or non-infected cells using the standard formulae. The OD values obtained from spectrochemical assay were compared with traditional Plaque assay and validated with Trypan blue assay. The data obtained from spectrochemical analysis showed similar trend as was achieved with traditional methods with a little variation in the sensitivity. The sensitivity variations are obvious due to the marked differences in the measurement unit and detection methods. However the newly introduced spectrochemical method showed superiority over the traditional methods because of its simple, label free, less time consuming measurement method and its suitability in the monitoring of large number samples.</p>",
"DOI": "10.5455/jabet.2018.d19",
"views": 1001,
"downloads": 310,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/10/178-1542113052-Figure1.jpg",
"caption": "Figure 1. Schematic illustrations of spectrochemical analysis of PPR infected vero cell.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/10/178-1542113052-Figure2.jpg",
"caption": "Figure 2. (a) Illustrations of the results of results of TCID50, (b) Optical microscopic image (5x) of non-infected vero cells and (c) PPR virus infected vero cells.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/10/178-1542113052-Figure3.jpg",
"caption": "Figure 3. TCID50 of the inoculum obtained from PPR virus infected vero cell culture fluid.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/10/178-1542113052-Figure4.jpg",
"caption": "Figure 4. (a) The image represents hydrolyzed formazan products with DMSO showing color intensity with decreasing concentrations (TCID50) of virus suspension, (b) represents MTT treated cell culture fluid taken out prior to the solubilization of formazan.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/11/10/178-1542113052-Figure5.jpg",
"caption": "Figure 5. Comparison between percent viability and percent infectivity obtained from Optical Density (OD) based spectrochemical analysis where X-axis represents virus concentrations (TCID50) and Y-axis represent percent viability/percent infectivity.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/20/10/178-1542113052-Figure6.jpg",
"caption": "Figure 6. Comparison between percent viability and percent\r\ninfectivity obtained from Trypan blue exclusion assay where X-axis\r\nrepresents virus concentrations (TCID50) and Y-axis represent percent viability/percent infectivity.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/20/10/178-1542113052-Figure7.jpg",
"caption": "Figure 7. Validation of spectrochemical assay with conventional trypan blue assay.",
"featured": false
}
],
"authors": [
{
"id": 130,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Mizanur",
"family_name": "Rahman",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 45
},
{
"id": 131,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Kumar Jyotirmoy",
"family_name": "Roy",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 45
},
{
"id": 132,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Mst. Khudishta",
"family_name": "Aktar",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 45
},
{
"id": 133,
"affiliation": [
{
"affiliation": "Bangladesh Agricultural Research Council, Farmgate, Dhaka 1215, Bangladesh"
}
],
"first_name": "Md. Rafiqul",
"family_name": "Islam",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 45
},
{
"id": 134,
"affiliation": [
{
"affiliation": "Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh"
}
],
"first_name": "Md. Abdul",
"family_name": "Kafi",
"email": "makafi2003@bau.edu.bd",
"author_order": 5,
"ORCID": "https://orcid.org/0000-0001-8126-024X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Md. Abdul Kafi, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh\u00022202, Bangladesh, Email:makafi2003@bau.edu.bd",
"article": 45
}
]
},
{
"id": 44,
"slug": "178-1539241375-insilico-analysis-of-g-aminobutyric-acid-transaminase-gaba-t-of-brassica-napus-rape",
"type": "original_article",
"manuscript_id": "178-1539241375",
"published": "2019-01-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/54/178-1539241375.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(1) : 04-09.",
"academic_editor": "Dr. Md Mahmodul Hasan, Erciyes University, Turkey.",
"cite_info": "Rahman S, Shahjahan M. Insilico analysis of γ- aminobutyric acid transaminase (GABA-T) of \r\nBrassica napus (Rape). J Adv Biotechnol Exp Ther. 2019; 2(1) : 04-09.",
"title": "Insilico analysis of γ- aminobutyric acid transaminase (GABA-T) of Brassica napus (Rape)",
"abstract": "<p>γ- aminobutyric acid (GABA) is an essential metabolite which plays a crucial role in signal transmission, stress metabolism, and some other activities also reported. Although the actual function of GABA shunt is not clearly understood. Three key enzymes, gamma aminobutyric acid transaminase (GABA-T), succinic semialdehyde dehydrogenase and succinic semialdehyde reductase are involved in GABA shunt mechanism en route from glutamate to the tricarboxylic acid cycle (TCA)which could pave the way of GABA shunt action. The enzyme gamma aminobutyric acid transaminase (GABA-T) could also play a key role in GABA shunt action by converting GABA to succinic semialdehyde (SSA).In this study, the protein sequence of γ-aminobutyric acid transaminase of <em>Brassica</em> <em>napus</em> <em>(Rape) was</em> <em>retrieved from </em>UniProt protein database and analyzed GABA-T enzyme using different bioinformatics tools and servers to analyze the physiochemical properties, amino acid composition, conformational states, and 3D structure. We found that our experimental protein sequence was very unstable, and the graph of Local Quality Estimate showed that the sequence was porn to mutation and value of Z score was above two in comparison with a non-redundant set of PDB structure. In addition, the phylogenetic tree revealed that GABA-T of <em>Dendrobium officinale</em><em>, </em><em>Phoenix dactylifera</em>, <em>Oryza sativa</em>, <em>Arabidopsis thaliana</em> and <em>Brassica napus </em>evolved from a common ancestor gene.</p>",
"DOI": "10.5455/jabet.2018.d18",
"views": 528,
"downloads": 109,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/10/178-1539241375-Figure1.jpg",
"caption": "Figure 1. Phylogenetic tree of GABA-T from soil bacteria and plant sources.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/10/178-1539241375-Figure2.jpg",
"caption": "Figure 2. Protein topology of the secondary structure.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/04/10/178-1539241375-Figure3.jpg",
"caption": "Figure 3. 3D structure of protein and Ramachandran plot.",
"featured": false
}
],
"authors": [
{
"id": 126,
"affiliation": [
{
"affiliation": "Bangladesh Livestock Research Institute Regional Station, Baghabari, Shahjadpur, Sirajganj-6770, Bangladesh"
}
],
"first_name": "Shahidur",
"family_name": "Rahman",
"email": "shahidur.blri@gmail.com",
"author_order": 1,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mr. Shahidur Rahman, Bangladesh Livestock Research Institute, Regional Station, Baghabari, Shahjadpur, Sirajganj\u00026770, Bangladesh, Email:shahidur.blri@gmail.com",
"article": 44
},
{
"id": 127,
"affiliation": [
{
"affiliation": "Bangladesh Livestock Research Institute Regional Station, Baghabari, Shahjadpur, Sirajganj-6770, Bangladesh"
}
],
"first_name": "Md",
"family_name": "Shahjahan",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 44
}
]
},
{
"id": 38,
"slug": "178-1536766636-the-possible-regulations-through-cross-generation-transmission-on-childhood-obesity",
"type": "editorial_article",
"manuscript_id": "178-1536766636",
"published": "2019-01-01",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/15/178-1536766636.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2019; 2(1) : 01-03.",
"academic_editor": "Dr. Akhi Moni, ABEx Bio-Research, Azampur, Dakkhinkhan, Dhaka-1230, Banglades",
"cite_info": "Ngoc VTN, Chu DT. The possible regulations through cross-generation transmission on \r\nchildhood obesity. J Adv Biotechnol Exp Ther. 2019; 2(1) : 01-03.",
"title": "The possible regulations through cross-generation transmission on childhood obesity",
"abstract": "",
"DOI": "10.5455/jabet.2018.d17",
"views": 613,
"downloads": 103,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/23/28/Figure_1.jpg",
"caption": "Figure 1. Regulations through cross-generation transmission on childhood obesity. Significant relationship of grandmother adiposity and grandchildren adiposity (A). Preconception cold exposure increases the formation and activity of thermogenic adipocytes resulting in reduction of fat mass and obesity in offspring (B).",
"featured": true
}
],
"authors": [
{
"id": 96,
"affiliation": [
{
"affiliation": "School of Dentistry, Hanoi Medical University, Hanoi, Vietnam"
}
],
"first_name": "Vo Truong Nhu",
"family_name": "Ngoc",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 38
},
{
"id": 97,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, Hanoi, Vietnam ."
}
],
"first_name": "Dinh-Toi",
"family_name": "Chu",
"email": "chudinhtoi.hnue@gmail.com",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-0935-2646",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Dinh-Toi Chu, Faculty of Biology, Hanoi National University of Education, Hanoi, Email: chudinhtoi.hnue@gmail.com",
"article": 38
}
]
}
]
}
],
"title": "2019",
"slug": "2019",
"order": 2
},
{
"id": 3,
"issues": [
{
"id": 2,
"title": "Vol1 Issue3",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2018",
"slug": "2018-vol1-issue3",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 35,
"slug": "178-1536554529-ocimum-herb-species-a-potential-treatment-strategy-for-diabetic-kidney-disease",
"type": "review_article",
"manuscript_id": "178-1536554529",
"published": "2018-09-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/15/178-1536554529.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(3) : 88-91.",
"academic_editor": "Dr. Masud Parvez, Department of Phamacology, Inje University, Busan, South Korea.",
"cite_info": "Indira, P. Akhi, M. Ocimum herb species: a potential treatment strategy for diabetic \r\nkidney disease. J Adv Biotechnol Exp Ther. 2018; 1(3) : 88-91.",
"title": "Ocimum herb species: a potential treatment strategy for diabetic kidney disease.",
"abstract": "<p>Ocimum genus most commonly termed as Tulsi, an aromatic plants belongs to family Lamiaceae. In addition to anti bacterial activity, anti-oxidant, anti diabetic, cardio protective, neuroprotective, radio-protective, anti-cancer, and free radical scavenging properties, quite a few studies have reported that this traditionally grown medicinal herb may become a potential therapy for diabetic kidney disease (DKD). This paper will significantly review Tulsi plants role in treatment of DKD to provide reference for future research.</p>",
"DOI": "10.5455/jabet.2018.d16",
"views": 1062,
"downloads": 165,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/15/09/178-1536554529-Figure_1.jpg",
"caption": "Figure 1. Therapeutic uses of Ocimum herb.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/15/09/178-1536554529-Figure_2.jpg",
"caption": "Figure 2. Anti-DKD effects of Ocimum herbs.",
"featured": false
}
],
"authors": [
{
"id": 88,
"affiliation": [
{
"affiliation": "Department of Biological Sciences, University of Ulsan, South Korea."
}
],
"first_name": "Indira",
"family_name": "Pandiri",
"email": "indirassspatchineelm@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-0963-9073",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Indira Pandiri, Department of Biological Sciences, University of Ulsan, South Korea, Email:\r\nindirassspatchineelm@gmail.com",
"article": 35
},
{
"id": 92,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Akhi",
"family_name": "Moni",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "",
"article": 35
}
]
},
{
"id": 28,
"slug": "178-1533298541-resveratrol-attenuates-inflammation-through-tristetraprolin-expression-in-human-hepatocytes",
"type": "original_article",
"manuscript_id": "178-1533298541",
"published": "2018-09-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2024/30/178-1533298541.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(3):78-82",
"academic_editor": "Hemant K. Sharma, PhD;\r\nCha University, South Korea",
"cite_info": "Moni A, Iqbal A, Uddin MJ. Resveratrol attenuates inflammation through tristetraprolin expression in human hepatocytes. J Adv Biotechnol Exp Ther. 2018; 1(3) : 78-82.",
"title": "Resveratrol attenuates inflammation through tristetraprolin expression in human hepatocytes",
"abstract": "<p>Resveratrol (Resv), a polyphenolic compound has the anti-inflammatory and antioxidant effects. Tristetraprolin (TTP) destabilizes mRNAs of pro-inflammatory cytokines. However, it was not clear how Resv exerts anti-inflammatory effects on hepatocytes. In this study, we used human hepatoma cell line (HepG2 cells) and analyzed samples using qRT-PCR. Resv increases TTP expression in hepatocytes. Resv attenuates LPS-induced inflammation through TTP expression in hepatocytes. In TTP deficient hepatocytes, LPS failed to attenuate TNFα, iNOS, and IL-6 mRNA expression whereas TTP level was still decreased. Our results suggest that Resv may attenuate inflammation in hepatocytes through TTP induction at least in part. This novel Resv-TTP signaling pathway provides new possibilities for the treatment of inflammatory diseases in liver.</p>",
"DOI": "10.5455/jabet.2018.d14",
"views": 645,
"downloads": 188,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/03/09/178-1533298541-Figure1.jpg",
"caption": "Figure 1. Resv increases TTP expression in HepG2 cells. Cells were treated with Resv (200 ng/ml) in time dependant manner (0, 2, 4, 8, and 12 h and the mRNA levels of TTP were measured by RT-PCR (A) and real time-RT-PCR analysis (C). Cells were treated with Resv in dose dependant manner (0, 50, 100, 200, and 400 ng/ml) and the mRNA levels of TTP were measured by RT-PCR (B) and real time-RT-PCR analysis (D). The representative bands are shown. Values are mean ± SEM (n = 3).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/03/09/178-1533298541-Figure2.jpg",
"caption": "Figure 2. Resv inhibits inflammation in HepG2 cells. Cells were pretreated with Resv (200 ng/ml) for 4 h and stimulated with LPS for 6 h. The mRNA levels of TTP (A), TNFα (B), IL-6 (C), and iNOS (D) were measured by real time-RT-PCR analysis. Values are mean ± SEM (n = 3).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/03/09/178-1533298541-Figure3.jpg",
"caption": "Figure 3. Silencing TTP failed to inhibit inflammation by Resv in HepG2 cells. (A) Cells were transfected with control siRNA and TTP siRNA for 24 h. Cells were pretreated with Resv (200 ng/ml) for 4 h and stimulated with LPS for 6 h. The mRNA levels of TTP (A), TNFα (B), IL-6 (C), and (D) iNOS were measured by real time-RT-PCR analysis. Values are mean ± SEM (n = 3).",
"featured": false
}
],
"authors": [
{
"id": 25,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Akhi",
"family_name": "Moni",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-5812-7755",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 28
},
{
"id": 26,
"affiliation": [
{
"affiliation": "Department of Genetic Engineering and Biotechnology, Shahjalal University of Science & Technology, Bangladesh"
}
],
"first_name": "Asif",
"family_name": "Iqbal",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 28
},
{
"id": 27,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": "hasan800920@gmail.com",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-2911-3255",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Jamal Uddin, PhD; Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, South Korea. email: hasan800920@gmail.com",
"article": 28
}
]
},
{
"id": 29,
"slug": "178-1535114371-molecular-characterization-and-phylogenetic-analysis-of-two-minnows-puntius-sarana-and-barbodes-gonionotus",
"type": "original_article",
"manuscript_id": "178-1535114371",
"published": "2018-09-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/55/178-1535114371_3.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(3) : 83-87.",
"academic_editor": "Dr. Parvin Mostari, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.",
"cite_info": "Sultana, S. Hossain, MS. Islam, MN. etal.Molecular characterization and phylogenetic analysis of two minnows, Puntius sarana and Barbodes gonionotus. J Adv Biotechnol Exp Ther. 2018; 1(3) : 83-87.",
"title": "Molecular characterization and phylogenetic analysis of two minnows, Puntius sarana and Barbodes gonionotus.",
"abstract": "<p>Two minnows, indigenous olive barb, Puntius sarana and exotic silver barb, Barbodes gonionotus are important fish species in Bangladesh. Therefore, it is essential to identify diversed population of these fish species for selective breeding programme. Sixty olive barb fish were collected from three different natural stocks (Mymensingh, Madaripur and Sylhet) and 20 silver barbs from hatchery stock of Jashore in Bangladesh. Out of 40 decamer primers tested, 5 primers were selected for the Polymerase Chain Reaction (PCR) based RAPD (Randomly Amplified Polymorphic DNA) analysis. Upon agarose gel electrophoresis, RAPD bands were scored as separately on the basis of their presence or absence for each sample and primer. A total of 43 polymorphic bands and highest proportion of polymorphic bands (62.79%) were found in the Madaripur populations. The gene diversity (0.2132±0.2067) and Shannon’s Information index (0.3161±0.2950) within populations were highest in Jashore stock. Among olive barb stocks, these values were higher in Sylhet stock. Besides, four populations segregated in two main clusters based on the Nei’s genetic distance. Indicating the segregation of two different species of minnows, the silver barb stock made one separate cluster while other three stocks of olive barb remained in another cluster. The present study exposed a distinct pattern of genetic variation and phylogenetic relatedness that would be helpful in selecting broodfish for genetic improvement as well as in conservation of these fish species.</p>",
"DOI": "10.5455/jabet.2018.d15",
"views": 578,
"downloads": 85,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/22/09/178-1535114371-Figure1.jpg",
"caption": "Figure 1. Sampling sites for natural (•) and hatchery (∆) populations of fish in the map of Bangladesh",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/22/09/178-1535114371-Figure2.jpg",
"caption": "Figure 2. Phylogenetic dendrogram constructed based on Nei’s (1972) genetic distance of different populations of Olive barb and Silver barb analyzed by RAPD markers.",
"featured": false
}
],
"authors": [
{
"id": 55,
"affiliation": [
{
"affiliation": "National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh"
}
],
"first_name": "Shirin",
"family_name": "Sultana",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 29
},
{
"id": 56,
"affiliation": [
{
"affiliation": "National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh"
}
],
"first_name": "Mohammad Shahdat",
"family_name": "Hossain",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 29
},
{
"id": 57,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Mohammad Nazrul",
"family_name": "Islam",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 29
},
{
"id": 58,
"affiliation": [
{
"affiliation": "National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh"
}
],
"first_name": "Md. Shahidul Islam",
"family_name": "Bhuiyan",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 29
},
{
"id": 59,
"affiliation": [
{
"affiliation": "National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh"
}
],
"first_name": "Md.",
"family_name": "Salimulla",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 29
},
{
"id": 60,
"affiliation": [
{
"affiliation": "National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh"
}
],
"first_name": "Jahangir",
"family_name": "Alam",
"email": "alamjahan@yahoo.com",
"author_order": 6,
"ORCID": "https://orcid.org/0000-0002-2693-419X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Jahangir Alam, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh, e-mail: \r\nalamjahan@yahoo.com",
"article": 29
}
]
},
{
"id": 26,
"slug": "178-1530154652-prophylactic-effects-of-vitamin-e-and-selenium-on-di-n-butyl-phthalate-induced-testicular-damage-in-prepubertal-rats",
"type": "original_article",
"manuscript_id": "178-1530154652",
"published": "2018-09-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/21/178-1530154652.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(3) : 65-71",
"academic_editor": "Dr. Hasan-Al-Faruque, Daegu Gyeonbuk Institute of Science and Technology, South Korea",
"cite_info": "Alam MS, Hoque MN. Prophylactic effects of vitamin E and selenium on di (n-butyl) phthalate-induced testicular damage in prepubertal rats. J Adv Biotechnol Exp Ther. 2018; 1(3) : 65-71.",
"title": "Prophylactic effects of vitamin E and selenium on di (n-butyl) phthalate-induced testicular damage in prepubertal rats",
"abstract": "<p>Di (n-butyl) phthalate (DBP), a potential endocrine disruptor, adversely affects male reproductive function via activation of oxidative stress. This study was conducted to investigate the protective effects of vitamin E (Vit E) and selenium (Se) on DBP-induced testicular damage. Prepubertal rats were given 500 mg DBP/kg alone and in combination with Vit E (250 mg/kg) & Se (1 mg/kg) by oral gavage for 7 days and sacrificed at day 1 (D1), D30 and D45 after administration. Testicular section of DBP-treated rats showed markedly distorted seminiferous tubules, no spermatids and a reduction in the thickness of their epithelial lining, compared to the control. In contrast, co-administration of Vit E & Se protected the seminiferous tubules and regained its normal architecture to the control level. To clarify whether spermatogenic cells differentiate into mature spermatids in the treated testes at the end of first wave of spermatogenesis, immunostaining for Hsc 70t, a specific marker for spermatids, was carried out. As a result, the increase in maturation of spermatids in Vit E & Se+DBP-treated testes, compared to the DBP-treated, was demonstrated. For example, the most advanced spermatids in the tubules from rats in the DBP-treated groups were steps 8-9 at D45 of recovery, while those of the DBP+Vit E & Se-treated were steps 14-19 that were more or less similar to the control group. These results show for the first time that prepubertal administration of Vit E & Se have protective effects on DBP-induced testicular damage and restoration of normal spermatogenesis.</p>",
"DOI": "10.5455/jabet.2018.d12",
"views": 2172,
"downloads": 133,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/24/10/178-1530154652-Figure1.jpg",
"caption": "Figure 1. Histopathology of testes after treatment. Vehicle-treated control (A). Only DBP-treated group (B). DBP+Vit E & Se-treated group (C), Vit E & Se-treated (D). Arrows indicate sloughed spermatogenic cells. Scale bar = 30 µm.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/24/10/178-1530154652-Figure2.jpg",
"caption": "Figure 2. Spermatogenic cell apoptotic index in the study groups. Vehicle-treated control (A). Only DBP-treated group (B). DBP+Vit E & Se-treated group (C). Quantification of apoptotic spermatogenic cells (D). Arrows indicate apoptotic cells. Scale bar = 30 µm. Values representing the number of apoptotic cells/mm2 cross sectional area of seminiferous tubules are expressed as the mean±S.E.M (n=4). Statistically significant differences between means from the control and treated groups were determined by ANOVA followed by Fisher’s PLSD test (*P<0.05; ** p<0.01, versus control).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/24/10/178-1530154652-Figure3.jpg",
"caption": "Figure 3. Maturation of spermatogenic cells in the study groups. (A) PAS-hematoxylin staining, in control, DBP-and DBP+Vit E & Se-treated rat testes at D30 (58-day-old) and D45 (73-day-old) after treatment. (B) Immunohistochemical staining for Hsc 70t protein, a specific marker for elongate spermatids, in control and DBP-and DBP+Vit E & Se-treated rat testes at D45 (73-day-old) after treatment. Note pretreatment with Vit E & Se could alleviate DBP-induced testicular injuries and significantly improved the maturation of spermagogenic cells after completion of first wave of spermatogenesis. Scale bar, A = 30 µm; B=20 µm.",
"featured": false
}
],
"authors": [
{
"id": 47,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Mohammad Shah",
"family_name": "Alam",
"email": "mdshahalam2004@yahoo.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-2657-9121",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Mohammad Shah Alam, Department of Anatomy and Histology, Bangabandhu Sheikh Mujibur Rahman \r\nAgricultural University, Gazipur-1706, Bangladesh, Email: mdshahalam2004@yahoo.com",
"article": 26
},
{
"id": 48,
"affiliation": [
{
"affiliation": "Department of Gynecology, Obstetrics and Reproductive Health, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur\u00021706, Banglades"
}
],
"first_name": "Md. Nazmul",
"family_name": "Hoque",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 26
}
]
},
{
"id": 27,
"slug": "178-1532086031-analysis-of-telomere-length-in-the-coronary-artery-to-determine-the-pathogenesis-of-coronary-artery-disease",
"type": "original_article",
"manuscript_id": "178-1532086031",
"published": "2018-09-20",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/24/178-1532086031.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(3) : 72-77",
"academic_editor": "Dr. Md. Nabiul Islam, Graduate School of Medicine, Yamaguchi University, Yamaguchi, Japan.",
"cite_info": "Shaik, MV. G, S. Analysis of telomere length in the coronary artery to determine the pathogenesis of coronary artery disease. J Adv Biotechnol Exp Ther. 2018; 1(3) : 72-77.",
"title": "Analysis of telomere length in the coronary artery to determine the pathogenesis of coronary artery disease",
"abstract": "<p>Endothelial dysfunction is one of the earliest pathological features in atherosclerosis. The current study designed to analyse the telomere length of human coronary artery derived endothelial cells to identify the relationship between telomere shortening and coronary artery disease (CAD)s. Coronary artery endothelial cells obtained from 30 patients with CAD and cell line were analysed by real time PCR method. Relative telomere and single-copy 36B4 gene (T/S) ratio which corresponds to the telomere length in coronary artery was significantly smaller in coronary artery disease patients than compared with control cell line. Flow cytometry analysis resulted endothelial cell count ranged from 0.55 x 106 to 0.96 x 106. Findings of this study suggest that telomere shortening in coronary artery endothelial cells play important role in the coronary artery disease.</p>",
"DOI": "10.5455/jabet.2018.d13",
"views": 1283,
"downloads": 134,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/45/10/178-1532086031-Figure1.jpg",
"caption": "Figure 1. Images of unprocessed and processed coronary arteries which were collected from the disposals after CABG in the patients with coronary artery disease.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/45/10/178-1532086031-Figure2.jpg",
"caption": "Figure 2. Agarose gel electrophoresis images of PCR DNA product derived from standard cell line and endothelial cells derived from coronary artery disease patients. A & B images are from two different samples and standards.\r\nResults showed that telomere length in endothelial cells from Cell line was longer compared to telomere length in endothelial cells from patients (p < 0.01; Students t – test). Relative T/S ratio which corresponds to the telomere length was less compared with cell line (Figure 3). Analysis by two-way ANOVA of telomere length from previously isolated DNA from endothelial cells of cell line and patients along with DNA isolated from sub-cultured cells showed significant differences (p < 0.01) between age, extent of sub-culturing, and interaction effects.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/45/10/178-1532086031-Figure3.jpg",
"caption": "Figure 3. Relative T/S ratio, which must be established for the experimental samples (coronary artery disease patients), reference DNA. The greater the relative T/S ratio is, the longer the telomere.",
"featured": false
}
],
"authors": [
{
"id": 54,
"affiliation": [
{
"affiliation": "Head, Genetics, Advanced Research Center, Narayana Medical College, Nellore, Andhrapradesh, India"
}
],
"first_name": "Mahaboob",
"family_name": "V Shaik",
"email": "drmahaboobvs@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-4059-1709",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Mahaboob V Shaik, Head, Genetics, Advanced Research Center, Narayana Medical College, Nellore,\r\nAndhrapradesh, India, Email: drmahaboobvs@gmail.com",
"article": 27
},
{
"id": 86,
"affiliation": [
{
"affiliation": "Director & Cardiologist, Narayana Medical Institutions, Nellore, Andhrapradesh, India."
}
],
"first_name": "Subrahmanyam",
"family_name": "G",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 27
}
]
}
]
},
{
"id": 3,
"title": "Vol1 Issue2",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2018",
"slug": "2018-vol1-issue2",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 25,
"slug": "178-1525624911-role-of-auxin-and-nitric-oxide-on-growth-and-development-of-lateral-root-of-plants-possible-involvement-of-exogenously-induced-phot1",
"type": "review_article",
"manuscript_id": "178-1525624911",
"published": "2018-05-11",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/16/178-1525624911.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(2) : 61-64.",
"academic_editor": "Dr. Md. Nabiul Islam, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.",
"cite_info": "Moni A, Islam MN, Uddin MJ. Role of auxin and nitric oxide on growth and development of lateral root of plants: possible involvement of exogenously induced Phot1. J Adv Biotechnol Exp Ther. 2018; 1(2) : 61-64.",
"title": "Role of auxin and nitric oxide on growth and development of lateral root of plants: possible involvement of exogenously induced Phot1",
"abstract": "<p>Development of root architecture including lateral root formation is a critical event for successful growth of plants. Auxin promotes the production of nitric oxide (NO) in roots, which is required for auxin-mediated root organogenesis. Inhibition of auxin transport reduced phototropin1 (Phot1)-GFP expression at the basal and central part of LRs, indicating role of auxin in Phot1 expression in lateral roots. However, the involvement of auxin-mediated NO on Phot1 expression in LR growth and development yet to be explored. This review gives an insight that auxin-mediated NO may lead to LR growth and development through interacting with Phot1.</p>",
"DOI": "10.5455/jabet.2018.d11",
"views": 1103,
"downloads": 88,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/03/08/178-1525624911-Figure1.jpg",
"caption": "Figure 1. Suggested schematic diagram. Blue light induces Phot1 expression which inhibits LR formation. On the other hand, exogenous auxin and NO directly helps in formation of LR. Exogenous source of auxin enhances Phot1expression which helps LR formation. Evidences indicate that there is a functional involvement of Auxin, NO, and Phot1 in LR growth.",
"featured": false
}
],
"authors": [
{
"id": 44,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Akhi",
"family_name": "Moni",
"email": null,
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-5812-7755",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 25
},
{
"id": 45,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Mohammad Nazrul",
"family_name": "Islam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 25
},
{
"id": 46,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": "hasan800920@gmail.com",
"author_order": 3,
"ORCID": "https://orcid.org/0000-0003-2911-3255",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Jamal Uddin, PhD; Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea, Email: hasan800920@gmail.com",
"article": 25
}
]
},
{
"id": 23,
"slug": "178-1519813435-antibacterial-and-cytotoxic-activity-of-seeds-of-white-hyacinth-bean-lablab-purpureus-l-sweet-white",
"type": "original_article",
"manuscript_id": "178-1519813435",
"published": "2018-05-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/11/178-1519813435.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(2) : 49-54",
"academic_editor": "Dr. Shahed Uddin Ahmed Shazib, University of Ulsan, South Korea.",
"cite_info": "Rahman SA, Akhter MS. Antibacterial and cytotoxic activity of seeds of white hyacinth bean (Lablab purpureus L. sweet ‘white’). J Adv Biotechnol Exp Ther. 2018; 1(2) : 49-54.",
"title": "Antibacterial and cytotoxic activity of seeds of white hyacinth bean (Lablab purpureus L. sweet ‘white’)",
"abstract": "<p>Seeds of white hyacinth bean available in Khulna, Bangladesh were investigated to assess bioactivities such as antibacterial and cytotoxic properties. For the evaluation of bioactivities solvent extraction was performed by using 50% ethanol and 50% methanol. In the present study, in vitro antibacterial screening was done by the method known as disc diffusion assay. Additionally, the cytotoxic activity was screened by using brine shrimp lethality bioassay. Both the extracts exhibited excellent activity against both gram positive and gram negative bacteria. For ethanolic extract of white hyacinth bean, maximum zone of inhibition was found 17.75 mm at 1000 μg/disc against Staphylococcus epidermidis and 16.75 mm against Staphylococcus aureus. On the other hand, for methanolic extract the maximum zone of inhibition was found as 17.25 mm for 1000 μg/disc against Escherichia coli and 16.75 mm against Pseudomonas aeruginosa. In comparison to ethanolic extract, methanolic extract was found to be more active against all tested microorganisms. Compared to vincristine sulphate (with LC50 of 0.99 μg/ml) both ethanolic and methanolic extracts of hyacinth bean seeds showed toxicity lower than 100 μg/ml and they were 34.67 μg/ml and 45.5 μg/ml, respectively. The LC50 values suggest moderate cytotoxicity of the tested samples. The experimental findings could be correlated with the traditional medicinal uses of the seeds of this plant and showed the rational for further investigation which would be required for isolating the possible bioactive constituents responsible for such activities.</p>",
"DOI": "10.5455/jabet.2018.d9",
"views": 1215,
"downloads": 190,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/53/09/178-1519813435-Figure1.jpg",
"caption": "Figure 1: Antibacterial activity of (A) ethanolic extract and (B)\r\nmethanolic extract of Lablab purpureus L. sweet ‘white’\r\n(Microorganisms vs. Zone of inhibition with standard error)",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/53/09/178-1519813435-Figure2.jpg",
"caption": "Figure 2: Plot of log concentration of (A) ethanolic and (B) methanolic extract of hyacinth bean seeds vs. Percent shrimp mortality",
"featured": false
}
],
"authors": [
{
"id": 42,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh."
}
],
"first_name": "Sheikh Ashikur",
"family_name": "Rahman",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 23
},
{
"id": 43,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh."
}
],
"first_name": "Md. Shamim",
"family_name": "Akhter",
"email": "shamim11akhter@gmail.com",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-5630-3906",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Md. Shamim Akhter, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, \r\nBangladesh. E-mil: shamim11akhter@gmail.com\r\nAcademic Editor and Affiliation: Dr. Shahed Uddin Ahm",
"article": 23
}
]
},
{
"id": 22,
"slug": "178-1519798391-expression-patterns-of-the-phosphoproteins-and-total-proteins-in-tlqp-21-a-vgf-derived-peptide-treated-sh-sy5y-cells",
"type": "original_article",
"manuscript_id": "178-1519798391",
"published": "2018-05-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/09/178-1519798391.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(2) : 43-48",
"academic_editor": "Dr. Masud Parvez, University Of Toronto, Canada.",
"cite_info": "Akhter MH, Requena JR.Expression patterns of the phosphoproteins and total proteins in TLQP-21 (a VGF derived peptide) treated SH-SY5Y cells. J Adv Biotechnol Exp Ther. 2018; 1(2) : 43-48.",
"title": "Expression patterns of the phosphoproteins and total proteins in TLQP-21 (a VGF derived peptide) treated SH-SY5Y cells",
"abstract": "<p>VGF (non-acronymic), belonging to a large granin family, gives rise to a number of bioactive peptides by proteolysis and exert an extensive array of biological effects on energy metabolism, pain modulation, gastric secretion function, reproduction, mood regulation, and, diabetes. Among VGF-derived peptides, TLQP-21 (The first four amino acids, in short TLQP (Thr-Leu-Gln-Pro) generalizes the nomenclature of the peptide by its length) is the most studied although little is known yet about downstream molecular mechanisms of action of VGF-derived peptides like TLQP-21. So here as a preliminary analysis, total protein expression was carried out in addition to the phosphoproteomic study of SH-SY5Y cells treated with TLQP-21, using the same cell extracts. Comparison of simple 1D SDS-PAGE gels stained with SYPRO® Ruby protein gel stain was carried out to assess whether changes in protein expression could be seen even at such low separation resolution. Expression of several proteins most likely Microtubule-associated protein 1B (MW 271 kDa), Tubulin beta chain (MW 57), Tubulin beta-4B chain (MW 50), Alpha-2-macroglobulin (MW 163), etc. in TLQP-21 treated and control samples was found significantly different, indicating that the peptide TLQP-21 exerts biological effects on SH-SY5Y cells. Further studies are required to validate the identity of the modulated proteins, obtained from mass spectrometry. Identification of modulated proteins after TLQP-21 treatment would open new avenues to discover the molecular mechanisms of its physiological and pharmacological state.</p>",
"DOI": "10.5455/jabet.2018.d8",
"views": 534,
"downloads": 76,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/16/09/178-1519798391-Figure1.jpg",
"caption": "Figure 1: Comparative selectivity of A). Pro-Q® Diamond phosphoprotein staining and B). SYPRO® Ruby (total) protein staining. Here the gel, at first, was stained with Pro-Q Diamond dye, then the same gel was subjected to post staining with SYPRO Ruby protein gel stain to picture out of all the proteins of the cell homogenates treated (T) and control (C) with the peptide showing differences in protein expression at position 1, 2, 3, 4 and 5.",
"featured": false
}
],
"authors": [
{
"id": 40,
"affiliation": [
{
"affiliation": "Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh."
}
],
"first_name": "Md. Shamim",
"family_name": "Akhter",
"email": "shamim11akhter@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0001-5630-3906",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Md. Shamim Akhter, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna,\r\nBangladesh. E-mil: shamim11akhter@gmail.com",
"article": 22
},
{
"id": 41,
"affiliation": [
{
"affiliation": "CIMUS Biomedical Research Institute, University of Santiago de Compostela-IDIS, Santiago de Compostela-15782, Spain."
}
],
"first_name": "Jesús R.",
"family_name": "Requena",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 22
}
]
},
{
"id": 20,
"slug": "178-1524741069-long-term-administration-of-gentamicin-affects-hemato-biochemical-parameters-and-liver-architecture-of-swiss-albino-mice",
"type": "original_article",
"manuscript_id": "178-1524741069",
"published": "2018-05-08",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/03/178-1524741069.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(2) : 29-35",
"academic_editor": "Dr. Hasan-Al-Faruque, Daegu Gyeonbuk Institute of Science and Technology, South Korea.",
"cite_info": "Jannat N, Amin T, Sultana N, etal. Long term administration of gentamicin affects hemato-biochemical parameters and liver architecture of Swiss Albino mice. J Adv Biotechnol Exp Ther. 2018; 1(2) : 29-35.",
"title": "Long term administration of gentamicin affects hemato-biochemical parameters and liver architecture of Swiss Albino mice",
"abstract": "<p>Gentamicin is most frequently used aminoglycoside antibiotic. Despite its wide use, the effects of gentamicin have not been clearly studied in relation to alteration of hemato-biochemical parameters and liver injury. In the present study, to evaluate the effects of gentamicin on behavioral, hematological, biochemical and morphological parameters of liver, Swiss albino mice were divided into 4 experimental groups (group A: control; group B: pharmacological dose; group C: pharmacological dose rate with chronic treatment, and group D: high dose with chronic treatment). All the mice from group C and D showed dullness, fearness, roughness of the body coat, anorexia and weakness. Liver weight and size were increased significantly in the mice of group C and D than that of group A or group B. Similarly, in hematological study, Total erythrocyte count (TEC), Total leukocyte count (TLC) and hemoglobin (Hb) % values were decreased significantly, whereas, Alanine amino transferase (ALT) values were increased significantly in the mice of group C and D. In addition, congestion and dark coloration with hepatomegaly were found in treated group C and D. Histological study revealed that the liver parenchyma showed central vein congestion, lymphocytic infiltration, irregular size of hepatocyte and dilatation of sinusoids of treated group C and D. Taken together, our current study suggests that although pharmacological dose of Gentamicin has no adverse effect on liver but chronic pharmacological dose or chronic high dose has serious adverse effect on liver. These observations lead us to postulate that gentamicin induces liver tissue damage after long term treatment.</p>",
"DOI": "10.5455/jabet.2018.d6",
"views": 1588,
"downloads": 150,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/09/Figure1.jpg",
"caption": "Figure 1: Blood chemical parameters in the control and gentamicin treated mice. TEC (A), TLC (B) and Hb (C) were significantly lower in group C and D, whereas, ALT (D) was significantly higher in group C and D than that of group A and B. (** p 0.01).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/09/Figure2.jpg",
"caption": "Figure 2: Weight and size of the liver in the control and gentamicin treated mice. Both Weight (A) and size (B) of the liver were significantly increased in group C and D than that of group A and B. (** p 0.01).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/42/09/Figure3.jpg",
"caption": "Figure 3: Photomicrograph of H & E stained histological section of liver of the (A) Control, Group A, or gentamicin injected mice with different dose rate (B) Group B, (C-D) Group C and (E-G) Group D.\r\n(A) showing intact liver parenchyma with centrally located central vein. (B) also showing intact liver parenchyma. (C-D) showing congestion around central vein and lymphocytic infiltration around bile canaliculi (black arrows), (E-G) showing dilatation of sinusoids (E), inclusions bodies in hepatic parenchyma (black arrow) (F) and tissue destruction and irregularity of liver hepatocytes (G). CV, central vein. Scale bar = 20 µm.",
"featured": false
}
],
"authors": [
{
"id": 32,
"affiliation": [
{
"affiliation": "Department of Anatomy & Histology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman \r\nAgricultural University, Gazipur-1706, Bangladesh"
}
],
"first_name": "Nure",
"family_name": "Jannat",
"email": null,
"author_order": 1,
"ORCID": "https://www.researchgate.net/profile/Nure-Jannat-3",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 20
},
{
"id": 33,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Tanjina",
"family_name": "Amin",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-9699-3374",
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 20
},
{
"id": 34,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Nasrin",
"family_name": "Sultana",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 20
},
{
"id": 35,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Mir Rubayet",
"family_name": "Jahan",
"email": null,
"author_order": 4,
"ORCID": "https://scholar.google.co.jp/citations?user=04BYdR4AAAAJ&hl=en",
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 20
},
{
"id": 36,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "M Rafiqul",
"family_name": "Islam",
"email": "rafiqah77@yahoo.com",
"author_order": 5,
"ORCID": null,
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "M Rafiqul Islam, DVM, MS, PhD, Professor, Department of Anatomy and Histology, Faculty of Veterinary Science,\r\nBangladesh Agricultural University, Mymensingh 2202, Bangladesh, Email: rafiqah77@yahoo.com",
"article": 20
}
]
},
{
"id": 21,
"slug": "178-1521008794-isolation-of-stem-cell-populations-from-whartons-jelly-sections-of-umbilical-cord-and-comparison-analysis-with-cord-blood-stem-cells",
"type": "original_article",
"manuscript_id": "178-1521008794",
"published": "2018-05-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/06/178-1521008794.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(2) : 36-42",
"academic_editor": "Dr. Gautam Deb, Bangladesh Livestock Research Institute, Bangladesh.",
"cite_info": "Shaik MV, K H, G S.Isolation of stem cell populations from wharton’s jelly sections of umbilical cord and comparison analysis with cord blood stem cells. J Adv Biotechnol Exp Ther. 2018; 1(2) : 36-42.",
"title": "Isolation of stem cell populations from wharton’s jelly sections of umbilical cord and comparison analysis with cord blood stem cells",
"abstract": "<p>Wharton’s jelly (WJ) is a gelatinous tissue exists in the umbilical cord contains stromal cells, termed as mesenchymal stromal cells (MSCs). Current study designed to characterize the stem cell population both within the cord blood and it’s wharton’s jelly tissue and it’s correlation to neonatal parameters. Both wharton’s jelly and cord blood and their CD34+ and CD90+ expression was enumerated by flow-cytometry. Neonatal parameters along with the mode of delivery were analyzed to correlate with CD34 and CD90 markers. From a total of 20 deliveries, CD34 and CD90 expression pattern showed varied in both samples. Gender and the expression of CD34 and CD90 markers doesn’t show any significant change. Whereas, correlation analysis of weight with CD34+ and CD90+ cell counts showed significant difference and it may have the effect on both cell counts. Neonatal parameters especially birth weight may have the effect on cell count of CD34 and CD90 in both CB and WJ. Current study provide a fast method for mesenchymal cell isolation from Wharton’s jelly tissue using the total length of the cord, which may be easily applied along with the UC blood, for the banking of UC.</p>",
"DOI": "10.5455/jabet.2018.d7",
"views": 787,
"downloads": 124,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/14/08/Figure_1.jpg",
"caption": "Figure 1. Flow cytometry analysis demonstrated that the cells were\r\npositive for CD90 and negligible/negative for CD34 and CD45 in\r\nwharton’s jelly and also positive for CD34 and negative for CD45 in\r\ncord blood.",
"featured": false
}
],
"authors": [
{
"id": 37,
"affiliation": [
{
"affiliation": "Head of Advanced Research Center, Asst. Professor, Department of Genetics & Stem cell Research, Narayana Medical College, Nellore, \r\nAndhrapradesh, India."
}
],
"first_name": "Mahaboob Vali",
"family_name": "Shaik",
"email": "drmahaboobvs@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-4059-1709",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dr. Mahaboob Vali Shaik, Head of Advanced Research Center, Asst. Professor, Laboratory of Genetics & Stem cell \r\nResearch, Narayana Medical College, Nellore, Andhrapradesh, India, Phone-9985335296, Email: drmahaboobvs@gmail.com",
"article": 21
},
{
"id": 38,
"affiliation": [
{
"affiliation": "Professor, Department of Obstetrics & Gynaecology, Narayana Medical College& Hospital, Nellore, Andhrapradesh, India."
}
],
"first_name": "Hymavathi",
"family_name": "K",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 21
},
{
"id": 39,
"affiliation": [
{
"affiliation": "Director & Cardiologist, Narayana Medical Institutions, Nellore, Andhrapradesh, India."
}
],
"first_name": "Subrahmanyam",
"family_name": "G",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 21
}
]
},
{
"id": 24,
"slug": "178-1520836022-morphometry-and-expression-of-immunoglobulins-containing-plasma-cells-in-the-harderian-gland-of-birds",
"type": "review_article",
"manuscript_id": "178-1520836022",
"published": "2018-05-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/14/178-1520836022.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(2) : 55-60",
"academic_editor": "Dr. Md Mahmodul Hasan Sohel, Erciyes University, Turkey.",
"cite_info": "Jahan MR, Islam MN, Khan MZI, etal. Morphometry and expression of immunoglobulins-containing plasma cells in the Harderian gland of Birds. J Adv Biotechnol Exp Ther. 2018; 1(2) : 55-60.",
"title": "Morphometry and expression of immunoglobulins-containing plasma cells in the Harderian gland of Birds",
"abstract": "<p>Johann Jacob Harder first described the Harderian gland in 1694 in deer. It is found in most terrestrial animals and is located within the variable aspects of the orbit. It is believed that this gland is involved in diverse functions. Among these, it has been held to be a site of immune response, a source of thermoregulatory lipids and pheromones, act as photoprotective organ as well as part of a retinal-pineal axis. In birds, this glad was reported first in sparrow in 1918. The Harderian gland is covered by capsule and the connective tissue septa that divide the gland into numerous unequal-sized lobes and lobules. Plasma cells are found in the interacinar space and the lumina of lobules. The recent studies suggest that the Harderian gland act as an immunopotent organ in birds, and that the gland in scavenging birds contains more immunoglobulin-containing plasma cells due to their scavenging nature. Moreover, this gland shows considerable species/strain differences in terms of macro anatomy, microanatomy as well as in the dynamics of immunoglobulin-containing plasma cells among different birds. In this review, these species and strain differences are discussed based on recent studies and several goals of future research are identified.</p>\r\n<p> </p>",
"DOI": "10.5455/jabet.2018.d10",
"views": 1197,
"downloads": 121,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/36/08/178-1520836022-Figure1.jpg",
"caption": "Figure 1: Schematic diagram for Harderian gland and its possible diverse function. This figure is adapted from [3], [9] and [10].",
"featured": false
}
],
"authors": [
{
"id": 49,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
},
{
"affiliation": "Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Yamaguchi, \r\nJapan"
}
],
"first_name": "Mir Rubayet",
"family_name": "Jahan",
"email": "rubayet.lucky@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-2867-5336",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mir Rubayet Jahan, DVM, MS, PhD, Associate Professor, Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh, Email: rubayet.lucky@gmail.com",
"article": 24
},
{
"id": 50,
"affiliation": [
{
"affiliation": "Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Yamaguchi, \r\nJapan"
}
],
"first_name": "Md. Nabiul",
"family_name": "Islam",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 24
},
{
"id": 51,
"affiliation": [
{
"affiliation": "Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh"
}
],
"first_name": "Md. Zahirul Islam",
"family_name": "Khan",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 24
},
{
"id": 52,
"affiliation": [
{
"affiliation": "Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Yamaguchi, \r\nJapan"
}
],
"first_name": "Akie",
"family_name": "Yanai",
"email": null,
"author_order": 4,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 24
},
{
"id": 53,
"affiliation": [
{
"affiliation": "Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Yamaguchi, \r\nJapan"
}
],
"first_name": "Koh",
"family_name": "Shinoda",
"email": null,
"author_order": 5,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 24
}
]
}
]
},
{
"id": 7,
"title": "Vol1 Issue1",
"cover": null,
"special_issue_title": "",
"special_issue_poster": null,
"current_issue": false,
"forthcoming_issue": false,
"special_issue_deadline": null,
"volume": "2018",
"slug": "2018-vol1-issue1",
"special_issue": false,
"topic_editors": [],
"info": "",
"issue_articles": [
{
"id": 15,
"slug": "178-1519046049-lipopolysaccharide-tolerance-attenuates-inflammatory-responses-by-increasing-heme-oxygenase-1-and-tristetraprolin-expression-in-raw2647-macrophages",
"type": "original_article",
"manuscript_id": "178-1519046049",
"published": "2018-01-31",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/51/178-1519046049.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(1) : 07-10.",
"academic_editor": "Md Nabiul Islam, PhD;\r\nYamaguchi University, Japan.",
"cite_info": "Moni A, Uddin MJ. Lipopolysaccharide tolerance attenuates inflammatory responses by increasing heme oxygenase-1 and tristetraprolin expression in Raw264.7 macrophages. J Adv Biotechnol Exp Ther. 2018; 1(1) : 07-10.",
"title": "Lipopolysaccharide tolerance attenuates inflammatory responses by increasing heme oxygenase-1 and tristetraprolin expression in Raw264.7 macrophages",
"abstract": "<p>Inflammation is associated with the progression of a variety of diseases. Lipopolysaccharides (LPS) tolerance is recognized to reduce proinflammatory responses. Heme oxygenase1 (HO1) and tristetraprolin (TTP) are induced by LPS tolerance and mediate the anti-inflammatory effects. However, it was not clear whether two molecules are linked in LPS tolerance. In this study, we sought to determine whether HO1 associates with TTP to mediate the anti-inflammatory effects of LPS tolerance. LPS treatment significantly increased mRNA and protein level of HO1 and TTP in a time dependant fashion while LPS significantly decreased mRNA and protein level of TNFα in Raw264.7 macrophages. LPS tolerance inhibited TNFα mRNA and protein while HO1 and TTP level was still increased. In HO1 deficient macrophages, LPS tolerance failed to attenuate TNFα mRNA expression but TTP level was still decreased. Our results suggest that HO1 and TTP are functionally linked in mediating anti-inflammatory effects of LPS tolerance. This novel LPS tolerance-HO1-TTP signaling pathway provides new possibilities for the treatment of inflammatory diseases.</p>",
"DOI": "10.5455/jabet.d5",
"views": 545,
"downloads": 133,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/59/08/Figure_1.jpg",
"caption": "Figure 1. LPS increases HO1 and TTP expression in Raw264.7 macrophages. (A-F) Cells were treated with LPS (100 ng/ml) in time dependant manner (0, 3, 6, 9, 18, and 24 h. (A-D) The levels of TNFα, HO-1, and TTP were measured by semi-qRT-PCR. ImageJ software was used for densitometry analysis. Values are mean ± SEM (n = 3). The representative bands are shown (A). (E) Cell supernatants were analyzed for TNFα by ELISA. Values are mean ± SD (n = 3). *p<0.05. (F) The protein levels of HO1 and TTP were analyzed by western blotting. The representative bands are shown.",
"featured": true
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/59/08/Figure_2.jpg",
"caption": "Figure 2. LPS tolerance inhibits inflammatory responses via TTP expression in Raw264.7 macrophages. (A) Cells were pretreated with LPS (100 ng/ml) for 24 h and then, challenged with LPS (1 ug/ml) for 6 h. (A). The levels of TTP, HO1, and TNFα mRNA were analyzed by RT-PCR. (B) The levels of TTP, and HO1 proteins were analyzed by western blotting. (C) Cell supernatants were analyzed for TNFα protein by ELISA. Values are mean ± SD (n = 3), *p<0.05. (D) Cells were transfected with HO1 siRNA, and then cells were pretreated with LPS (100 ng/ml) for 24 h and challenged with LPS (1ug/ml) for another 6 h. The levels of TTP, HO-1, and TNFα mRNA were analyzed by RT-PCR.",
"featured": false
}
],
"authors": [
{
"id": 12,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
}
],
"first_name": "Akhi",
"family_name": "Moni",
"email": "akhimoni840818@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-5812-7755",
"corresponding": true,
"co_first_author": true,
"co_author": false,
"corresponding_author_info": "Akhi Moni, PhD; ABEx Bio-Research, 3161, East Azampur, Imam Bukhari (RA) Road, Azampur, Dakkhinkhan, Dhaka 1230, Bangladesh. e-mail: akhimoni840818@gmail.com",
"article": 15
},
{
"id": 13,
"affiliation": [
{
"affiliation": "ABEx Bio-Research Center, Azampur, Dakkhinkhan, Dhaka-1230, Bangladesh"
},
{
"affiliation": "Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea"
}
],
"first_name": "Md Jamal",
"family_name": "Uddin",
"email": "hasan800920@gmail.com",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0003-2911-3255",
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 15
}
]
},
{
"id": 18,
"slug": "178-1514390013-role-of-drug-metabolic-enzymes-and-transporters-in-drug-drug-interactions-between-antiretroviral-and-antituberculosis-drugs",
"type": "review_article",
"manuscript_id": "178-1514390013",
"published": "2018-01-25",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/53/178-1514390013.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(1) : 17-24.",
"academic_editor": "Dr. Md Ataur Rahman, Korea Institute of Science and Technology, South Korea.",
"cite_info": "Parvez MM, Kaisar N, Shin JG. Role of drug metabolic enzymes and transporters in drug-drug interactions between antiretroviral and antituberculosis drugs. J Adv Biotechnol Exp Ther. 2018; 1(1) : 17-24.",
"title": "Role of drug metabolic enzymes and transporters in drug-drug interactions between antiretroviral and antituberculosis drugs",
"abstract": "<p>Drug-drug interactions is one of the major determinant in drug development and clinical applications. Individual differences in pharmacokinetics may cause extensive variability in drug efficacy, toxicity and adverse drug reactions, and represent a major concern in drug development. According to world health organization, an estimated 1.2 million patients were diagnosed with HIV/TB coinfection in 2015, and approximately 400,000 coinfected individuals were died in 2016. During treatment of tuberculosis and human immunodeficiency virus (HIV) coinfections, a combination of multiple therapeutic drugs is used, which is potential for interactions between coadministered drugs. Drug metabolizing enzymes and transporters plays vital role in the disposition, pharmacokinetics as well as efficacy and toxicity of many antituberculosis (anti-TB) and antiretroviral (ARVs) drugs. The current understanding of drug-drug interactions involving the first-line antituberculosis and antiretroviral (ARVs) drugs are reviewed and summarized in this article emphasizing the role of drug transporters and metabolic enzymes. It is found that several clinical drug-interactions are caused by the phase I and II metabolic enzymes (cytochrome-P-450s, CYPs, UDP-glucuronosyltransferases, UGTs) and uptake/ efflux transporters, particularly permeability glycoprotein, brest cancer resistance protein, multidrug resistance protein and solute carrier transporters). Overall, this study will be helpful to understand the drug-interactions between the ARV or anti-TB drug regimen or with concomitantly used drugs at a glance. By understanding the unique functions and characteristics of enzymes and transporters, it may use in the management of drug interactions in the treatment of HIV and TB.</p>",
"DOI": "10.5455/jabet.d2",
"views": 1092,
"downloads": 94,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/29/08/Figure1.jpg",
"caption": "Figure 1. Involvement of drug metabolizing enzymes and transporters in pharmacokinetics. This figure represents the involvement of metabolic enzymes and transporters can produce drug-drug interaction by inhibition/ induction of the corresponding enzymes and transporters in the different tissues (GI, Liver, Brain and Kidney).",
"featured": true
}
],
"authors": [
{
"id": 29,
"affiliation": [
{
"affiliation": "Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, Busan, Republic of Korea."
},
{
"affiliation": "Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Canada."
}
],
"first_name": "Md Masud",
"family_name": "Parvez",
"email": "masud.parvez@utoronto.ca",
"author_order": 1,
"ORCID": "https://scholar.google.com/citations?user=45gf75QAAAAJ&hl=en",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Masud Parvez, PhD; Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, Busan, Republic of Korea and Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, \r\nCanada.. e-mail: masud.parvez@utoronto.ca",
"article": 18
},
{
"id": 30,
"affiliation": [
{
"affiliation": "Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, Busan, Republic of Korea."
}
],
"first_name": "Nazia",
"family_name": "Kaisar",
"email": null,
"author_order": 2,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 18
},
{
"id": 31,
"affiliation": [
{
"affiliation": "Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, Busan, Republic of Korea."
},
{
"affiliation": "Department of Clinical Pharmacology, Inje University Busan Paik Hospital, Busan, Republic of Korea"
}
],
"first_name": "Jae-Gook",
"family_name": "Shin",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 18
}
]
},
{
"id": 17,
"slug": "178-1513541446-choice-of-samples-in-extracellular-microrna-research-which-fraction-is-better-exosomal-or-nonexosomal",
"type": "review_article",
"manuscript_id": "178-1513541446",
"published": "2018-01-15",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/47/178-1513541446.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(1) : 11-16.",
"academic_editor": "Md Nurul Haider, PhD; Bangladesh Agricultural University, Bangladesh",
"cite_info": "Sohel MMH. Choice of samples in extracellular microRNA research: Which fraction is better- exosomal or nonexosomal? J Adv Biotechnol Exp Ther. 2018; 1(1) : 11-16.",
"title": "Choice of samples in extracellular microRNA research: Which fraction is better- exosomal or nonexosomal?",
"abstract": "<p>MicroRNAs (miRNAs) are a species of noncoding RNA that post-transcriptionally regulates the expression of target mRNAs via degradation or translational repression. Recently, miRNAs in biofluids got extraordinary research priority in the field of noninvasive biomarker development because of their association with pathophysiological events, noninvasive way to obtain, and are markable stability in the extracellular harsh environment. Although several hypotheses have been proposed, the precise mechanisms of cellular release of extracellular miRNAs are not properly understood. In addition, it is often confusing to the researchers, which fraction of biofluid (based on release mechanisms) is suitable for biomarker development research. This review will briefly introduce extracellular miRNAs (EC miRNAs) and their release mechanisms. Furthermore, based on recent evidence, it will also summarize and suggest the appropriate fraction of biofluid to work with for biomarker development.</p>",
"DOI": "10.5455/jabet.d3",
"views": 693,
"downloads": 81,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/38/29/178-1513541446-Figure1.jpg",
"caption": "Figure 1. Biogenesis and extracellular release of miRNAs. Mature miRNAs bind with protein complex (primarily AGO2) either to form RISC complex or released in the extracellular environment. They can also be loaded to exosomes and released. Similarly, EC miRNAs can be encapsulated via microvesicles or bind with HDL and released from cells to the extracellular environment. This figure is adapted from [4].",
"featured": true
}
],
"authors": [
{
"id": 28,
"affiliation": [
{
"affiliation": "Department of Animal Science, Faculty of Agriculture, Erciyes University, Kayseri 38039, Turkey"
},
{
"affiliation": "Genome and Stem Cell Centre, Erciyes University, Kayseri 38039, Turkey."
}
],
"first_name": "Md Mahmodul Hasan",
"family_name": "Sohel",
"email": "sohel.mmh@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0003-2224-085X",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Md Mahmodul Hasan Sohel, Department of Animal Science, Faculty of Agriculture, Erciyes University, Kayseri 38039, Turkey, e-mail: sohel@erciyes.edu.tr, or sohel.mmh@gmail.com",
"article": 17
}
]
},
{
"id": 16,
"slug": "178-1513778846-dna-fingerprinting-and-molecular-diversity-analysis-for-the-improvement-of-brinjal-solanum-melongena-l-cultivars",
"type": "original_article",
"manuscript_id": "178-1513778846",
"published": "2018-01-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/11/178-1513778846.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(1) : 01-06.",
"academic_editor": "Md Saidur Rahman, PhD\r\nChung-Ang University, South Korea.",
"cite_info": "Sultana S, Islam MN, Hoque ME. DNA fingerprinting and molecular diversity analysis for the improvement of brinjal (Solanum melongena L.) cultivars. J Adv Biotechnol Exp Ther. 2018; 1(1) : 01-06.",
"title": "DNA fingerprinting and molecular diversity analysis for the improvement of brinjal (Solanum melongena L.) cultivars",
"abstract": "<p>An investigation was conducted to assess genetic diversity of Bangladesh Agricultural Research Institute (BARI) released 11 varieties of brinjal (BARI begun 1, BARI begun 4-10, BARI hybrid begun 1, 3 and 4) using PCR-based randomly amplified polymorphic DNA (RAPD) markers. A total of 44 distinct DNA amplified bands were observed for five primers (OPB-04, OPB-08, OPD-02, OPP-13 and OPW-08) with an average of 5.6 polymorphic bands per primer. The overall gene diversity was detected 0.216 and level of polymorphism was found 63.64%. The pair wise inter-variety similarity indices ranged from 67.03% to 97.61%. The UPGMA dendrogram segregated 11 genotypes of brinjal into two main clusters. The first major cluster had only one genotype (BARI begun 6) and the second major cluster had rest of ten genotypes. BARI begun 6 vs BARI begun 1 showed the highest Nei’s genetic distance (0.5261) as they are released from different parental origin. On the other hand, BARI begun 9 vs BARI begun 7 varietal pair showed lowest genetic distance (0.0465) as they are released from same parental origin. The experiment reveals genetic diversity and relatedness among 11 brinjal genotypes which may be informative for the future varietal identification and genetic improvement of this vegetable crop.</p>",
"DOI": "10.5455/jabet.d1",
"views": 1186,
"downloads": 124,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/14/08/Figure1.jpg",
"caption": "Figure 1. DNA fingerprinting profile of 11 brinjal varieties based on five RAPD primers, (A) OPB-04, (B) OPB-08, (C) OPD-02, (D) OPP-13 and (E) OPW-08. Lane 1: BARI begun 1, lane 2: BARI begun 4, lane 3: BARI begun 5, lane 4: BARI begun 6, lane 5: BARI begun 7, lane 6: BARI begun 8, lane 7: BARI begun 9, lane 8: BARI begun 10, lane 9: BARI hybrid begun 1, lane 10: BARI hybrid begun 3, lane 11: BARI hybrid begun 4. Ms: M1=100 bp DNA ladder and M2: 1 kb DNA ladder.",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2024/14/08/Figure2.jpg",
"caption": "Figure 2. The UPGMA (Unweighted pair group method of arithmetic mean)\r\ndendrogram based on Nei‟s (1972) genetic distance within 11 brinjal varieties\r\naccording to RAPD analysis.",
"featured": true
}
],
"authors": [
{
"id": 14,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Sharmin",
"family_name": "Sultana",
"email": null,
"author_order": 1,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "",
"article": 16
},
{
"id": 15,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Mohammad Nazrul",
"family_name": "Islam",
"email": "nazrul2010@sau.edu.bd",
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-5692-3566",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Mohammad Nazrul Islam, PhD\r\nDepartment of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh",
"article": 16
},
{
"id": 16,
"affiliation": [
{
"affiliation": "Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh"
}
],
"first_name": "Md Ekramul",
"family_name": "Hoque",
"email": null,
"author_order": 3,
"ORCID": null,
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 16
}
]
},
{
"id": 19,
"slug": "178-1513778843-a-protein-isolation-method-for-western-blot-to-study-histones-with-an-internal-control-protein",
"type": "short_communication",
"manuscript_id": "178-1513778843",
"published": "2018-01-05",
"pdf_file": "https://jabet.bsmiab.org/media/pdf_file/2023/08/178-1513778843.pdf",
"journal_reference": "J Adv Biotechnol Exp Ther. 2018; 1(1) : 25-28",
"academic_editor": "Dr. Md Mahmodul Hasan Sohel, Erciyes University, Turkey.",
"cite_info": "Chu DT, Tao Y. A protein isolation method for western blot to study histones with an internal control protein. J Adv Biotechnol Exp Ther. 2018; 1(1) : 25-28.",
"title": "A protein isolation method for western blot to study histones with an internal control protein",
"abstract": "<p>Histone modification is one of the attractive targets for epigenetic studies. However, current methods to extract chromatin-associated proteins for western blot of histone modification have some weaknesses such as the loss of housekeeping proteins. In this study, we are presenting a simple method to isolate nuclear protein for studying histone modification by immunoblotting with housekeeping proteins. This method provided high protein concentration from minute tissue samples and importantly, it allowed us to detect acetylated histones together with internal control proteins such as β actin.</p>",
"DOI": "10.5455/jabet.d4",
"views": 1538,
"downloads": 179,
"figures": [
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/14/23/178-1513778843-Figure1.jpg",
"caption": "Figure 1. Our method to isolate soluble and nuclear proteins. Soluble and nuclear proteins were isolated using ice-cold radioimmune precipitation assay buffer (RIPA) modified from a protocol we had done [6-8]. RIPA buffer includes 50 mM Tris, 150 mM NaCl, 1 mM EDTA, 0.1% SDS, 1% Nonodet P-40 (NP40), 0.25% sodium deoxycholate, and 0.02% sodium azide in ddH2O. 150mg of frozen mouse inguinal fat tissues (ING) or frozen liver was homogenized in 1ml of ice-cold RIPA supplemented with phenylmethylsulfonyl fluoride (PMSF – Sigma, P7626), protease inhibitor cocktail (Sigma, P8340) and PhosStop (PhosStop - Roche, 4906837001) (1). Homogenized samples were incubated on ice for 1h (2), and sonicated by VibraCell Sonicator at 40% of the sonicator’s amplitude with 3 times for 1 min (3), and then centrifuged at 10000g for 15 min at 4ºC (4). Upper liquid phase (Soluble - So) was transferred into a new 1.5ml Eppendorf for membrane and plasma proteins (5a). Pellet plus around 200µl under liquid phase (Nuclear - Nu) was kept for nuclear proteins (5b). In case of fat tissues, the fat fraction on the top of samples was carefully removed after centrifuging. Pellet was dissolved in under liquid phase by pipetting. In the control method, histone extraction was done from 150mg of frozen ING and liver of mice according to the Abcam’s protocol (http://www.abcam.com/protocols/histone-extraction-protocol-for-western-blot) using Triton Extraction Buffer, this buffer contains PBS 1X, 0.5% Triton X100 (V/V), 2mM PMSF, 0.02% Sodium azide (W/v) (NaN3). Histones were extracted by 0.2N HCl over night at 4°C (Acid). Protein concentration in both methods was measured using Bradford reagent (Sigma, B6916).",
"featured": false
},
{
"figure": "https://jabet.bsmiab.org/media/article_images/2023/14/23/178-1513778843-Figure2.jpg",
"caption": "Figure 2. Our protein isolation method gave strong signals of acetylated histone 3 and internal control (β-actin). Acetylated histone 3 (H3) and β-actin (B. Actin) in protein isolated by our and control methods were detected by western blot as described [9, 10, 6]. Blots were incubated with antibodies against acetyl histone 3 (rabbit anti acetyl H3 – Millipore, Cat. # 06-599) (1:1000), β actin (mouse anti β actin –Abcam, Ab6276) (1:10000). Specific antibody-antigen complexes were detected using fluorescent-labeled secondary antibodies (goat anti-rabbit IRDye 800, Rockland, 611-132-122; goat anti-mouse IRDye 800, Rockland, 610-730-124). Bands were visualized and quantified using the Odyssey imaging system (Licor Bioscience).",
"featured": true
}
],
"authors": [
{
"id": 23,
"affiliation": [
{
"affiliation": "Faculty of Biology, Hanoi National University of Education, Hanoi, Vietnam"
}
],
"first_name": "Dinh-Toi",
"family_name": "Chu",
"email": "chudinhtoi.hnue@gmail.com",
"author_order": 1,
"ORCID": "https://orcid.org/0000-0002-4596-2022",
"corresponding": true,
"co_first_author": false,
"co_author": false,
"corresponding_author_info": "Dinh-Toi Chu, \r\nFaculty of Biology, Hanoi National University of Education, Viet Nam\r\ne-mail: chudinhtoi.hnue@gmail.com",
"article": 19
},
{
"id": 24,
"affiliation": [
{
"affiliation": "College of Food Science and Technology, Nanjing Agricultural University, Nanjing 8 210095, China"
}
],
"first_name": "Yang",
"family_name": "Tao",
"email": null,
"author_order": 2,
"ORCID": "https://orcid.org/0000-0001-5276-8546",
"corresponding": false,
"co_first_author": false,
"co_author": true,
"corresponding_author_info": "",
"article": 19
}
]
}
]
}
],
"title": "2018",
"slug": "2018",
"order": 1
}
]
