Table 2. Primer sequences for cytokine gene analysis using real-time PCR.
|
No. |
Primer |
Orientation |
Sequences (5’- 3’) |
Size (bp) |
Reference |
|
1. |
β-actin |
Forward |
AGAAAATCTGGCACCACACC |
395 |
[33] |
|
Reverse |
CTCCTTAATGTCACGCACGA |
||||
|
2. |
TNF-α |
Forward |
TGCTTGTTCCTCAGCCTCTT |
186 |
[33]
|
|
Reverse |
ATGGGCTACAGGCTTGTCACT |
||||
|
3. |
NF-κB |
Forward |
ATCCCATCTTTGACAATCGTGC |
153 |
[34]
|
|
Reverse |
CTGGTCCCGTGAAATACACCTC |
||||
|
4. |
IL-6 |
Forward |
GTACATCCTCGACGGCATC |
384 |
[33] |
|
Reverse |
AGCCACTGGTTCTGTGCCT |
||||
|
5. |
IL-10 |
Forward |
GCCTAACATGCTTCGAGATC |
206 |
[35] |
|
Reverse |
TGATGTCTGGGTCTTGGTTC |