Table 2. Conditions of PCR for LEP1 and LEP2.
|
Steps |
Temperature and duration |
Cycle |
|
Initial denaturation Denaturation Annealing Extension Final Extension Holding |
95oC for 5 minutes 94oC for 15 seconds 51oC for 30 seconds 72oC for 30 seconds 72oC for 5 minutes 4oC ∞ until use |
1 cycle
35 cycles
1 cycle - |