Table 1. Primers used in PCR amplification
|
Primer Name |
Primer Sequence (5'-3') |
Amplicon size (bp) |
Annealing temperature |
|
MC1R-P1 |
Forward: AGCCATGAGTTGAGCAGGAC |
567 |
62°C |
|
Reverse: ACCCAGATGGCTGCAATGAT |
|||
|
MC1R-P2 |
Forward: CTGTGGACCGCTACATCTCC |
641 |
62°C |
|
Reverse: GAAGGCCTGTGTACCCTCAC |