Table 2. Summary of NGS running metrics.
|
Run |
Level |
Density (K/mm2) |
Clusters PF (%) |
Phas/ Prephas (%) |
Reads |
Reads PF |
% >= Q30 |
Cycles Err Rated |
Aligned (%) |
Error Rate (%) |
Intensity Cycle 1 |
% Intensity Cycle 20 |
Yield Total (G) |
Projected Total Yield (G) |
% Perfect [Num Cycles] |
% <= 3 errors [Num Cycles] |
|
Run 1 |
Read 1 |
1212 +/- 20 |
83.5 +/- 1.1 |
0.080 / 0.091 |
6.27 |
5.24 |
95.7 |
150 |
4.4 +/- 0.1 |
0.57 +/- 0.02 |
347 +/- 33 |
114.1 +/- 9.7 |
0.8 |
0.8 |
82.8 [150] |
97.1 [150] |
|
Read 2 |
1212 +/- 20 |
83.5 +/- 1.1 |
0.000 / 0.000 |
6.27 |
5.24 |
89.8 |
0 |
0.0 +/- 0.0 |
0.00 +/- 0.00 |
934 +/- 98 |
0.0 +/- 0.0 |
0 |
0 |
0.0 [5] |
0.0 [5] |
|
|
Read 3 |
1212 +/- 20 |
83.5 +/- 1.1 |
0.011 / 0.018 |
6.27 |
5.24 |
92.9 |
150 |
4.3 +/- 0.1 |
0.73 +/- 0.05 |
292 +/- 24 |
125.5 +/- 3.3 |
0.8 |
0.8 |
78.4 [150] |
95.1 [150] |
|
|
Run 2 |
Read 1 |
1342 +/- 56 |
60.9 +/- 21.5 |
0.111 / 0.095 |
6.73 |
4.17 |
91.3 |
150 |
1.5 +/- 0.0 |
0.98 +/- 0.33 |
405 +/- 27 |
89.9 +/- 25.4 |
0.6 |
0.6 |
66.1 [150] |
93.5 [150] |
|
Read 2 |
1342 +/- 56 |
60.9 +/- 21.5 |
0.000 / 0.000 |
6.73 |
4.17 |
52.3 |
0 |
0.0 +/- 0.0 |
0.00 +/- 0.00 |
279 +/- 24 |
0.0 +/- 0.0 |
0 |
0 |
0.0 [5] |
0.0 [5] |
|
|
Read 3 |
1342 +/- 56 |
60.9 +/- 21.5 |
0.062 / 0.017 |
6.73 |
4.17 |
88.9 |
150 |
1.4 +/- 0.0 |
1.03 +/- 0.10 |
294 +/- 30 |
103.8 +/- 22.1 |
0.6 |
0.6 |
67.0 [150] |
91.8 [150] |
|
|
Run 3 |
Read 1 |
1194 +/- 13 |
85.4 +/- 1.4 |
0.074 / 0.083 |
6.27 |
5.35 |
95.4 |
150 |
2.2 +/- 0.0 |
0.62 +/- 0.15 |
380 +/- 35 |
116.0 +/- 7.8 |
0.8 |
0.8 |
76.3 [150] |
97.1 [150] |
|
Read 2 |
1194 +/- 13 |
85.4 +/- 1.4 |
0.000 / 0.000 |
6.27 |
5.35 |
87.3 |
0 |
0.0 +/- 0.0 |
0.00 +/- 0.00 |
199 +/- 18 |
0.0 +/- 0.0 |
0 |
0 |
0.0 [5] |
0.0 [5] |
|
|
Read 3 |
1194 +/- 13 |
85.4 +/- 1.4 |
0.009 / 0.020 |
6.27 |
5.35 |
91.3 |
150 |
2.1 +/- 0.0 |
0.84 +/- 0.04 |
309 +/- 26 |
122.3 +/- 5.1 |
0.8 |
0.8 |
75.7 [150] |
93.8 [150] |