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Table 4. Sperm motility and morphology with different diluters in the nitrogen vapor technique.

Diluters

Motility (%)

Morphology (%)

Motile sperm

Progressive motility

Slow

motility

Bent tail

Coiled tail

 

DMRI

Distal Droplet

Pre-frozen

88.17±15.80c

67.92±8.55c

0.49±0.64a

4.39±1.82a

0.29±0.31a

1.35±0.84a

1.20±0.73a

TFE

72.69±15.92b

49.13±17.16b

5.89±9.23b

5.82±3.97a

0.39±0.25a

2.93±1.48b

2.40±2.17a

Andromed

27.5±19.24a

11.76±10.76a

1.19±1.33a

13.49±5.97b

0.82±0.60b

1.59±0.50a

4.87±1.77c

Triladyl

70.07±16.40b

39.54±13.40b

5.38±3.13a

5.80±3.26a

0.55±0.60a

3.40±1.66b

3.18±1.57b

p-value

0.00

0.00

0.06

0.00

0.11

0.00

0.00

 N=5, results are presented in mean±SEM. Different letters (a, b, c) indicate differences among freezing groups (p<0.05).