Table 4. Sperm motility and morphology with different diluters in the nitrogen vapor technique.
Diluters |
Motility (%) |
Morphology (%) |
|||||
Motile sperm |
Progressive motility |
Slow motility |
Bent tail |
Coiled tail
|
DMRI |
Distal Droplet |
|
Pre-frozen |
88.17±15.80c |
67.92±8.55c |
0.49±0.64a |
4.39±1.82a |
0.29±0.31a |
1.35±0.84a |
1.20±0.73a |
TFE |
72.69±15.92b |
49.13±17.16b |
5.89±9.23b |
5.82±3.97a |
0.39±0.25a |
2.93±1.48b |
2.40±2.17a |
Andromed |
27.5±19.24a |
11.76±10.76a |
1.19±1.33a |
13.49±5.97b |
0.82±0.60b |
1.59±0.50a |
4.87±1.77c |
Triladyl |
70.07±16.40b |
39.54±13.40b |
5.38±3.13a |
5.80±3.26a |
0.55±0.60a |
3.40±1.66b |
3.18±1.57b |
p-value |
0.00 |
0.00 |
0.06 |
0.00 |
0.11 |
0.00 |
0.00 |
N=5, results are presented in mean±SEM. Different letters (a, b, c) indicate differences among freezing groups (p<0.05).