Table 3. Comparison of cryopreserved sperm motility and morphology in nitrogen vapor and commercial freezer protocols.
|
Motility (%) |
Morphology (%) |
|||||||
|
Motile sperm |
Progressive motility |
Slow motility |
Bent tail |
Coiled tail
|
Distal midpiece reflex |
Distal Droplet |
Proximal Droplet |
|
|
Pre-frozen semen |
97.84(a)± 1.94 |
71.62(a) ± 3.98 |
0.26(a) ± 0.08 |
3.78±1.78 |
0.24±0.10 |
2.12±0.50 |
1.98±0.48 |
23.10±9.23 |
|
Minitube |
65.94 (b)± 4.65 |
45.54(b) ± 3.64 |
2.43(b) ± 0.36 |
6.62± 0.85 |
0.33±0.06 |
3.36±0.38 |
2.60±2.69 |
11.10±2.69 |
|
Nitrogen vapor technique |
57.49(b) ± 5.67 |
38.70(b) ± 4.04 |
3.83(b) ± 0.63 |
7.55±1.05 |
0.51±0.10 |
2.97±0.33 |
2.92±0.37 |
9.30±2.74 |
|
p-value |
0.044 |
0.005 |
0.012 |
0.285 |
0.188 |
0.344 |
0.517 |
0.170 |
Minitube-TurboFreezer, (n=5, results are presented in Mean±SEM). Different letters (a, b) indicate differences among freezing groups (p<0.05).