Table 2. Optimizing the distance of semen straws over liquid nitrogen.
Motility (%) |
Morphology (%) |
||||||
Motile sperm |
Progressive motility |
Bent tail
|
Coiled tail |
Distal midpiece reflex |
Distal Droplet |
Proximal Droplet |
|
Pre-frozen semen |
90.40a± 2.50 |
51.23a ± 8.79 |
9.60a ± 2.50 |
0.33 ± 0.24 |
1.27± 0.50 |
0.07 ± 0.07 |
1.17 ± 0.24 |
0.5 inch |
38.47c ± 7.34 |
21.43b ± 2.80 |
7.37b ± 1.80 |
0.83 ± 0.47 |
1.43 ± 0.15 |
3.33± 1.23 |
13.97 ± 9.51 |
1.5 inch |
47.13 c ± 3.37 |
24.50b ± 1.29 |
4.63a ± 0.43 |
0.27 ± 0.07 |
1.43 ± 0.20 |
2.30 ± 0.30 |
4.30 ± 0.36 |
1.6 inch |
62.67b ± 1.12 |
38. 97a ± 1.10 |
4.30a ± 0.85 |
0.13 ± 0.03 |
1.37 ± 0.23 |
3.20 ± 1.06 |
18.17 ± 12.38 |
2.0 inch |
38.10c ± 1.97 |
23.10b ± 3.95 |
7.83b ± 2.03 |
0.30± 0.10 |
1.67 ± 0.18 |
3.70 ± 0.61 |
6.53 ± 0.76 |
3.0 inch |
35.03c ± 8.25 |
18.07b ± 4.20 |
8.13b ± 1.38 |
0.43 ± 0.20 |
1.60 ± 0.25 |
4.20 ± 1.27 |
5.53 ± 0.99 |
p-value |
0.000 |
0.001 |
0.020 |
0.228 |
0.633 |
0.204 |
0.726 |
n=5, results are presented in mean±SEM. Different letters (a, b, c) indicate differences among freezing groups (p<0.05).