Table 1. Primers are used in the amplification process.
|
Primer sequences |
Annealing Temperature |
Primer length (bp) |
PCR product size (bp) |
|
|
rs1799793 |
F-TGTAAAACGACGGCCAGTCAACGTCTGC ATCGACTC R-CAGGAAACAGCTATGACGTAAATGGTGC TCCCACTC |
60 C° |
36 |
897 |
|
rs238406 |
F-TGTAAAACGACGGCCAGTACTCTTGTATCCCTGCATTAAG R-CAGGAAACAGCTATGACCAGAAGTATGAGCAGATGGATAG |
60 C° |
40 |
696 |
SNP: single nucleotide polymorphism, bp: Base pairs, PCR: polymerase chain reaction, F: forward, R: reverse