Table 3. Primer sequences, lengths, and amplicon sizes for Real-time PCR.
|
Gene |
Primer name |
Primer sequence (5′-3′) |
Primer length |
Amplicon size |
|
DRMT1 |
Forward TGCTGATTCTGCTTCTGGGG Reverse TGGCGGTTCTCCATGTTCTT |
20 bp 20 bp |
138 bp |
|
|
RBMY1A1 |
Forward GAAGCCGTGGAGGAACAAGA Reverse TCTTGCCACAGCAGAAGGAG |
20 bp 20 bp |
179 bp |
|
|
PRY |
Forward AGCTTCTGAGAGACCCAGGA Reverse TTGGGATTGTTGAGGCCCAT |
20 bp 20 bp |
120 bp |
|
|
B-actin |
Forward AGAGCTSCGAGCTGCCTGAC Reverse AGCACTGTGTTGGCGTACAG |
20 bp 20 bp |
184 bp |
|