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Table 3. Primer sequences, lengths, and amplicon sizes for Real-time PCR.

Gene

Primer  name

Primer sequence (5′-3′)

Primer  length

Amplicon size

DRMT1

Forward                    TGCTGATTCTGCTTCTGGGG

                              Reverse                    TGGCGGTTCTCCATGTTCTT

20 bp

20 bp

 

138 bp

RBMY1A1

Forward                   GAAGCCGTGGAGGAACAAGA

Reverse                    TCTTGCCACAGCAGAAGGAG

20 bp

20 bp

179 bp

PRY

Forward                   AGCTTCTGAGAGACCCAGGA

Reverse                    TTGGGATTGTTGAGGCCCAT

20 bp

20 bp

120 bp

B-actin

Forward                   AGAGCTSCGAGCTGCCTGAC

Reverse                    AGCACTGTGTTGGCGTACAG

20 bp

20 bp

184 bp