DMRT1, RBMY, and AZFb genes polymorphism and expression role in azoospermia susceptibility

Table 2. Polymorphism and microdeletion detection with specific primers.

Gene	Primer name	Primer sequence (5′-3′)	Amplicon size (bp)
DMRT1	Forward outer	CCTACTAACACACAGGGAGCTGAATTCTG	185
	Reverse outer	CACCAGAAATTTAGTGGCGTAAATGACAC	185
	Forward inner (C allele)	CTGCTAGAGGCTCCAGAAAGAAATGC	126
	Reverse inner (T allele)	GTCTCTGTCAAGGTGTCTGTGGGGCTA	112
RBMY	Forward outer	CCTGTCAACAAAGGCGGAGGAAAGCAG	303
	Reverse outer	GGCGGATTCCTTTGGTCTTTTCTGCAAA	303
	Reverse inner (T allele)	CCTCTTGCAGGTGGTGCACCATGAGA	153
	Forward inner (G allele)	CATATTATTTTACCAACCCTGCAGGGACCT	206
AZFb	sY127F	GGCTCACAAACGAAAAGAAA	274
AZFb	sY127R	CTGCAGGCAGTAATAAGGGA	274
AZFb	sY127F	GTCTGCCTCACCATAAAACG	301
AZFb	sY127R	ACCACTGCCAAAACTTTCAA	301