Interaction of epitopic missense variants of VEGFA with therapeutic monoclonal antibodies

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Table 3. Effects of epitopic missense variants on VEGFA-Ranibizumab interaction.

SNP_ID	Amino acid position^a	Amino acid change	Antibody chains	∆∆G (Kcal/mol)^b				MutaBind2	PPcheck^c
SNP_ID	Amino acid position^a	Amino acid change	Antibody chains	mCSM-PPI2	SAAMBE-3D	MutaBind2	BeAtMusic V1.0	MutaBind2	PPcheck^c
WT			H						-449.45
			L						-19.53
rs114262569	82	R>W	H	1.294	1.13	3.1	-0.01	3.31	-458.53
			L					0.9	-19.54
rs367757959	82	R>Q	H	1.291	1.9	1.86	0.97	1.99	-457.15
			L					1.22	-19.54
rs767587788	83	I>L	H	1.15	0.72	0.76	0.78	0.93	-447.01
			L					0.93	-19.56
rs1284410244	87	Q>R	H	-0.057	1.17	0.73	1.07	0.26	-449.03
			L					1.3	-19.96
rs1275152500	88	G>S		-1.276	0.52	2.8	1.01	2.64	-465.75
								0.56	-19.44

^a Amino acid positions were calculated excluding the signal peptide (26 amino acids).

^bPositive values of ∆∆G indicate decreasing affinity.

^cThe calculations with PPcheck for wild type structure was done with the Swiss model prepared 3D model.