Interaction of epitopic missense variants of VEGFA with therapeutic monoclonal antibodies

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Table 2. Effects of epitopic missense variants on VEGFA-Bevacizumab interaction.

SNP_ID	Amino acid position^a	Amino acid change	Antibody chains	∆∆G (Kcal/mol)^b				MutaBind2	PPcheck^c
SNP_ID	Amino acid position^a	Amino acid change	Antibody chains	mCSM-PPI2	SAAMBE-3D	MutaBind2	BeAtMusic V1.0	MutaBind2	PPcheck^c
WT			H						-382.96
			L						-18.77
rs114262569	82	R>W	H	0.885	1.32	2.54	0.28	2.55	-373.65
			L					0.98	-18.83
rs367757959	82	R>Q	H	1.328	2.27	1.92	0.67	2.17	-367.21
			L					1.3	-18.76
rs767587788	83	I>L	H	0.995	0.63	1.26	0.67	1.26	-382.66
			L					0.96	-18.88
rs1275152500	88	G>S	H	-1.035	0.48	3.44	1.31	2.55	-396.01
			L					0.59	-18.74
rs1456457746	92	G>R	H	2.034	1.35	3.55	1.33	3.61	-412.72
			L					1.2	-19.03

^aAmino acid positions were calculated excluding the signal peptide (26 amino acids).

^bPositive values of ∆∆G indicate decreasing affinity.

^cThe calculations with PPcheck for wild type structure was done with the Swiss model prepared 3D model.