Biofilm formation by the interaction of fungi (Candida tropicalis) with various bacteria

Table 1. Biofilm formation for each treatment was measured by CV staining and MTT method.

Species	CV values^*	CV category	MTT values^*	MTT category
C. tropicalis	2.412 ± 0.825^b	HBF	1.525 ± 0.473^b	ICP
C. tropicalis x MRSA	2.173 ± 0.515^c		1.508 ± 0.380^c	ICP
C. tropicalis x S. aureus	2.008 ± 0.212^c		1.481 ± 0.364^c	ICP
C. tropicalis x A. baumannii	1.913 ± 0.366^c		1.423 ± 0.304^c	ICP
P. aeruginosa	1.854 ± 0.488^b		1.239 ± 0.500^b	NCP
C. tropicalis x P. aeruginosa	1.163 ± 0.243^c		1.144 ± 0.095^c	NCP
A. baumannii	0.975 ± 0.471^b		1.114 ± 0.313^b	NCP
MRSA	0.653 ± 0.332^b		0.995 ± 0.165^b	NCP
S. aureus	0.512 ± 0.224^b		0.978 ± 0.349^b	NCP
Control group	0.048 ± 0.008^a	-	0.079 ± 0.014^a	-

Tukey post hoc test showed=^aControl group. ^bSingle-species treatment. ^cMixed-species treatment. CV values = displays the absorbance value with Crystal violet assay with *P-value < 0.05 from the data considered statistically significant. MTT values = displays the absorbance value with the MTT assay with *P-value < 0.05 from the data considered statistically significant. HBF = high biofilm-forming. ICP = increased cell proliferation. NCP = normal cell proliferation. Data expressed as mean ± SD.