Table 1. qRT-PCR mix preparation
|
Name |
Reagent |
Volume\test |
No.\test |
|
qRT-PCR mix |
qRT-PCR reaction mix |
17 µL |
N = n + 2 |
|
|
qRT-PCR enzyme mix |
3 µL |
|
N = n + 2 denotes the number of tests, with n denoting the number of samples to be tested and 2 denoting the negative and positive controls.