Genes - SNPs

Primers

PCR

Product (bp)

Restriction Enzyme

Restriction Enzyme Product (bp)

Reference

OPN-rs1126616

Forward:5′TACCCTGATGCTACAGACGAGG3′

Reverse:5′CTGACTATCAATCACATCGGAATG3′

252

AluI

C 147+105

T 105; 61+44

[29]

MMP 3-rs35068180

Forward:5'CTTCCTGGAATTCACATCACTGCCACCACT3'

Reverse:5'GGTTCTCCATTCCTTTGATGGGGGGAAAGA3'

129

PsyI

6A/6A-129

6A/5A-129, 97+32

 5A/5A-97+32

[30]

OCN-rs1800247

Forward:5′CCGCAGCTCCCAACCACAATAAGCT3′

Reverse:5′CAATAGGGCGAGGAGT3′

253

HindIII

TT 232; 21

TC 253; 232+21 CC 253

[31]

MGP-rs4236

Forward:5′TCAATAGGGAAGCCTGTGATG3′
Reverse:5′AGGGGGATACAAAATCAGGTG3′

173

Eco477

CC 127+46

CT 173+127

TT 173

[32]

CYP24A1-rs2248359

Forward:5′AGTTAGGAAATGCGCCTTGAG3′

Reverse:5′GGATCAGGTTGAAAGGATTCG3′

326

SacII

C 100+226

T 326

[33]