Table 2. PCR Condition for genotyping of KISS1 gene rs372790354 alleles: G>A and rs4889 alleles G>A.
|
No. of cycle |
Time |
Tm |
NO. of Program |
|
1 35 - -
1 |
30sec 5 sec
10sec
20sec
|
94 94 -- 56 60 72 55-95 |
Preheating Denaturation Annealing (rs372790354) (rs4889) Extension HRM |