Effect of KISS1 gene variants (rs372790354 G>A and rs4889 G>A) on kisspeptin in patients with polycystic ovary syndrome in Iraq

Table 1. PCR primer for genotyping of KISS1 gene rs372790354 alleles: G>A and rs4889 alleles G>A.

Product size	Sequence of primer	Primers sequences of SNPs
91	Forward: CTGGGGTGCGGGGATCTG	HRM rs4889 G>A
91	Reverse: GACCTCGCTGTCCCCGCC	HRM rs4889 G>A
N	CTGGGGTGCGGGGATCTGGCGGCTGTGGGGGGCGGACA CCCGGCTGCTGGGGGCTCCCGGAGCTCTCGGGGGGCGGGGACAGCGAGGTC	Wild rs4889 Allele G
N	CTGGGGTGCGGGGATCTGGCGGCTGTGGGGGGCGGACAGGCCCG GCTGCTGGAGGCTCCCGGAGCTCTCGGGGGGCGGGGACAGCGAGGTC	Mutant rs4889 Allele A
78	Forward: CTCAGTCCTGGCCTGGG	HRM rs372790354 G>A
78	Reverse: CCCTCTGGACATTCACCCA	HRM rs372790354 G>A
N	CTCAGTCCTGGCCTGGGCAGGAGTCTGGCGGAG CCTCTGAGGTGACGAGACCACCTGGCTGGGTGAATGTCCAGAGGG	Wild rs372790354 Allele G
N	CTCAGTCCTGGCCTGGGCAGGAGTCTGGCGGAGCCTCTGAG GTGACAAGACCACCTGGCTGGGTGAATGTCCAGAGGG	Mutant rs372790354 Allele A