Table 2. The restriction enzymes, their cutting sequence, and fragment length resulted from digestion.
|
SNP |
Restriction enzyme |
Cutting sequence |
Product size and genotypes |
|
rs7144366 |
Sfc I |
5’-C▼TRYAG-3’ |
TT: 97bp TC: 97bp+66bp+31bp CC: 66bp+31bp |
|
rs8192917 |
BsmA I
|
5’-GTCTCN▼...-3’ |
TT: 420bp TC: 420+328bp+92bp CC: 328bp+92bp |
|
rs2236338 |
BsmF I
|
5’-GGGAA(N)10▼..-3’ |
AA: 400bp AG:400bp+272bp+128bp GG: 272bp+128bp |