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Table 2.  Comparative analysis of four pairs of primers by conventional RT-PCR.

Sl .No.

Primer name

Positive response in RT-PCR

Negative response in RT-PCR

P-value

1

NF/NR gene

26

4

.002 (sig.)

2

N1/N2 gene

25

5

3

F1b/F2b gene

21

9

4

F1/F2 gene

14

16