Table 1. Oligonucleotide primers set used for the PPRV detection.
|
Genes targeted |
Primer’s name |
Sequences (5´-3´) |
Amplicon size |
References |
|
Designed NF/NR gene |
PPRVNF |
GCTCTGTGATTGCGGCTGAGC |
402bp |
Designed, GenBank Ac. No. GQ122187.1 |
|
PPRVNR |
CCT GGT CCT CCA GAATCTTGGCC |
|||
|
N1/N2 gene |
PPRVN1(F) |
GATGGTCAGAAGATCTGCA |
463bp |
[9] |
|
PPRVN2(R) |
CTTGTCGTTGTAGACCTGA |
|||
|
F1/F2 gene |
PPRVF1(F) |
ATCACAGTGTTAAAGCCTGT AGAGG |
372bp |
[7] |
|
PPRVF2(R) |
GAGACTGAGTTTGTGACCTA CAAG |
|||
|
F1b/F2b gene |
PPRVF1b |
AGTACAAAAGATTGCTGATCACAGT |
448bp |
[3] |
|
PPRVF2b |
GGGTCTCGAAGGCTAGGCCCGAATA |