Table 8. Phytochemical analysis for five wild macrofungi.
|
S. No. |
Phytoconstituents |
Cantharellus cibarius |
Phellinus pectinatus |
Laccaria laccata |
Trametes versicolor |
Gloeophyllum sepiarium |
|
EtOH extract |
MeOH extract |
EtOH extract |
EtOH extract |
MeOH extract |
||
|
1 |
Test for Alkaloids a) Mayer’s test b) Dragendorff’s test c) Wagner’s test |
Negative Negative Negative |
Positive Positive Negative |
Negative Negative Negative |
Negative Negative Negative |
Positive Positive positive |
|
2 |
Test for terpenoids |
Positive |
Positive |
Positive |
Positive |
Positive |
|
3 |
Test for steroids |
Positive |
Positive |
Positive |
Positive |
Positive |
|
4 |
Test for Glycosides a) Libermann Burchard’s test b) Keller-Killiani test |
Positive
Positive |
Negative
Positive |
Positive
Negative |
Negative
Positive |
Negative
Positive |
|
5 |
Test for Flavonoids a) Alkaline reagent test (b) Ferric chloride test |
Negative
Negative |
Positive
Positive |
Negative
Positive |
Negative
Positive |
Positive
Negative |
|
6 |
Test for Phenolic compounds a) Ferric chloride test b) Lead acetate test |
Negative Negative |
Positive Negative |
Positive Negative |
Negative Negative |
Negative Negative |
|
7
|
Test for carbohydrates a) Fehling’s test b) Benedict’s test c) Molisch's test d) Barfoed test |
Negative Negative Negative Negative |
Positive Positive Positive Positive |
Negative Negative Negative Positive |
Positive Negative Positive Negative |
Positive Positive Positive Positive |
|
8 |
Test for Amino acids a) Ninhydrin test b) Millon's test c) Xanthoproteic |
Negative Negative Negative |
Negative Negative Positive |
Positive Negative Negative |
Positive Negative Negative |
Negative Negative Positive |
|
9 |
Test for Saponins a) Froth test |
Negative |
Negative |
Negative |
Negative |
Negative |
*Phytochemical test methods are useful for the identification of secondary metabolites, active against many microbes. * Ethanol extract - EtOH, Methanol extract - MeOH