Table 1. Biochemical test for identification of Genus.
|
Name of the tests |
Observations |
Results |
Remarks |
|
Gram-staining |
Red color on slide under microscope |
- |
Gram negative. |
|
Catalase |
Presence of bubble on slide |
+ |
Catalysis of H2O2 |
|
Oxidase |
Purple color on filter paper |
+ |
Production of Cytochrome c oxidase. |
|
Indole |
No color change |
- |
Cannot utilize tryptophan. |
|
MR (Methyl red) |
Red color in test tubes |
+ |
Production of mixed acids such as lactic acid. |
|
VP (Vogesprosker) |
Pink, red color in test tubes |
+ |
Production of acetone in broth culture. |
|
Glucose fermentation |
Yellow color in test tubes |
+ |
Metabolism of carbohydrates such as glucose, dextrose. |
|
Citrate test |
Intense blue color in test tube |
+ |
Utilization of citrate as carbon source. |