Treatment

Sterilizing agent

Time

Plant part

T1

70% Ethanol

1 min

Stem, root, leaf rhizome

T2

70% Ethanol + 0.5% Sodium Hypochlorite

1min, 1min

Leaf, Stem

T3

70% Ethanol + 1% Sodium Hypochlorite

1min 30 sec, 1 min 30 sec

Leaf, stem, root, rhizome

T4

70% Ethanol + 1.5% Sodium Hypochlorite

1 min 30 sec, 2 min

Leaf, stem, roots

T5

70% Ethanol + 2 % Sodium Hypochlorite + 70 % Ethanol

1 min, 2-3 min, 60 sec

Leaf, stem and roots, rhizome