HPTLC fingerprinting analysis of phytoconstituents from Bixa orellana and Beta vulgaris plant pigment

Table 5. Peak table with retention factor (Rf) values of alkaloid compounds of Bixa orellana extract.

BOD		BOM		BOA		BOC
RF value	Assigned substance	RF value	Assigned substance	RF value	Assigned substance	RF value	Assigned substance
0.03	Unknown	0.09	Nicotine		Unknown	0.03	Unknown
0.13	Unknown	0.21	Unknown	0.06	Unknown	0.14	Unknown
0.24	Unknown	0.31	Strychnine	0.2	Unknown	0.46	Alkaloid 1
0.26	Unknown	0.4	Unknown	0.31	Strychnine	0.52	Unknown
0.31	Strychnine	0.92	Unknown	0.53	Unknown	0.75	Unknown
0.5	Unknown			0.89	Unknown
0.58	Unknown			0.94	Unknown
0.81	Unknown
0.93	Unknown

BOD- Dichloromethane extract of Bixa orellana, BOM- Methanol extract of Bixa orellana, BOA- Acetone extract of Bixa orellana, BOC- Chloroform extract of Bixa orellana.