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Table 5. Peak table with retention factor (Rf) values of alkaloid compounds of Bixa orellana extract.

BOD

BOM

BOA

BOC

RF value

 

Assigned substance

RF value

Assigned substance

RF value

Assigned substance

RF value

Assigned substance

0.03

Unknown

0.09

Nicotine

 

Unknown

0.03

Unknown

0.13

Unknown

0.21

Unknown

0.06

Unknown

0.14

Unknown

0.24

Unknown

0.31

Strychnine

0.2

Unknown

0.46

Alkaloid 1

0.26

Unknown

0.4

Unknown

0.31

Strychnine

0.52

Unknown

0.31

Strychnine

0.92

Unknown

0.53

Unknown

0.75

Unknown

0.5

Unknown

 

 

0.89

Unknown

 

 

0.58

Unknown

 

 

0.94

Unknown

 

 

0.81

Unknown

 

 

 

 

 

 

0.93

Unknown

 

 

 

 

 

 

BOD- Dichloromethane extract of Bixa orellana, BOM- Methanol extract of Bixa orellana, BOA- Acetone extract of Bixa orellana, BOC- Chloroform extract of Bixa orellana.