HPTLC fingerprinting analysis of phytoconstituents from Bixa orellana and Beta vulgaris plant pigment

Table 4. Preliminary tests for Bixa orellana and Beta vulgaris extract.

Phytoconstituents		BVAE	BVAA	BVM	BVAM	BOD	BOM	BOA	BOC
Alkaloid	Mayer’s test	+	+	-	++	+	-	-	+
	Wagner’s test	+	+	-	+	++	+	+	+
	Hager’s test	-	++	-	-	++	+	++	-
Carbohydrates	Molisch’s test	++	++	+	+++	+	++	+	++
	Fehling’s test	+++	++	+	+	++	++	++	+++
	Benedict’s test	++	++	++	+	+	++	++	+
Saponins	Foam test	++	++	+	+	+	++	+	+
Proteins and amino acids	Millon’s test	-	-	-	+	-	-	-	-
Proteins and amino acids	Ninhydrin test	++	+	-	+	+	-	+	+
Phytosteroids and terpenoid	Libermann-Burchard’s test	++	++	++	+	+	++	++	+
Fixed oil and fats	Spot test	++	+	++	+	+	+	-	-
Phenolic and flavonoids/Tannin	Ferric chloride test	+++	-	+	++	++	+	+	++
	Gelatin test	+	-	+	+	+	-	+	-
	Lead acetate test	+++	++	++	++	+	+	+	++
Diterpenes	Copper acetate test	++	-	-	++	-	++	++	-