Table 1. List of the primers used in the real time PCR.
|
Gene |
Template strand |
Product length (bp) |
|
|
COX-2 |
FW |
GTACTCCCGATTGAAGCCCC |
138 |
|
RV |
TCGTGTAGCGGTGAAAGTGG |
||
|
TNF-α |
FW |
GGGTGTGAGAAGAGAGATGGG |
342 |
|
RV |
GGCCAGAGGGCTGATTAGAG |
||
|
GAPDH |
FW |
GCTGAGTACGTCGTGGAGTC |
455 |
|
RV |
CCCATTCCCCAGCTCTCATA |
FW: forward primer; RV: reverse primer. Tm: melting temperature.