Table 1. Experimental design for the determination of the hypoglycemic activity of the aqueous leaf extracts of P. zanzibarica and O. europaea Subspecies africana in Alloxan-induced diabetic mice.
|
Treatment Groups |
Treatment |
|
Normal control |
Physiologic saline only (10 mL/kg body weight; i.p)+ Physiologic saline (10 mL/kg body weight; p.o) |
|
Negative control |
Alloxan (150 mg/kg body weight; i.p) + Physiologic saline (10 mL/kg body weight; p.o) |
|
Positive control |
Alloxan (150 mg/kg body weight; i.p) + Insulin (1 IU/kg body weight; i.p) |
|
Experimental group 1 |
Alloxan (150 mg/kg body weight ; i.p) + Extract (50 mg/kg body weight ; p.o) |
|
Experimental group 2 |
Alloxan (150 mg/kg body weight ; i.p) + Extract (100 mg/kg body weight ; p.o) |
|
Experimental group 3 |
Alloxan (150 mg/kg body weight ; i.p) + Extract (150 mg/kg body weight ; p.o) |
Extract: Aqueous leaf extract of P. zanzibarica or Aqueous leaf extract of O. europaea subspecies africana; i.p: Intraperitoneal injection; p.o: Per os (oral route of administration). The tails of experimental mice were swapped with 70 % ethanol, and blood samples were collected at the start of the experiment and repeated after 1 hour, 2 hours, 3 hours, and 4 hours. A glucose analyser model (Hypoguard, Woodbridge, England) was used to measure blood glucose levels.