Table 1. Primer sequences.
|
Genes |
Primers |
Primer sequences (5’-3’) |
|
β-globin NC_000011.10 |
Globin F |
CAACTTCATCCACGTTCACC |
|
Globin R |
GAAGAGCCAAGGACAGGTAC |
|
|
GSTP1 NC_000011.10 |
GSTP1 Me-F1 |
TCGGTTAGTTGCGCGGCGATTTC |
|
GSTP1 Me-F2 |
TTCGGGGTGTAGCGGTCGTC |
|
|
GSTP1 Me-R |
CGACGAAACTCCAACGAAAAC |
|
|
GSTP1 Un-F1 |
AGTTGTGTGGTGATTTTGGGG |
|
|
GSTP1 Un-F2 |
GATGTTTGGGGTGTAGTGGTTGT |
|
|
GSTP1 Un-R |
CCAACAAAAACCTCACAACCT |
|
|
CDKN2A/p16 NC_000009.12 |
P16 Me-F1 |
TTATTAGAGGGTGGGGCGGATCGC |
|
P16 Me-R1 |
CCACCTAAATCGACCTCCGACCG |
|
|
P16 Me-F2 |
TTATTAGAGGGTGGGGCGGATCGC |
|
|
P16 Me-R2 |
GACCCCGAACCGCGACCGTAA |
|
|
P16 Un-F1 |
TTATTAGAGGGTGGGGTGGATTGT |
|
|
P16 Un-R1 |
CCACCTAAATCAACCTCCAACCA |
|
|
P16 Un-F2 |
TTATTAGAGGGTGGGGTGGATTGT |
|
|
P16 Un-R2 |
CAACCCCAAACCACAACCATAA |
|
|
RASSF1A NC_000003.12 |
RASSF1A Me-F |
GGTTTTGCGAGAGCGCGTTTA |
|
RASSF1A Me-R |
ACGCTAACAAACGCGAACCGA |
|
|
RASSF1A Un-F1 |
GGGGTTTTGTGAGAGTGTGTTTAG |
|
|
RASSF1A Un-F2 |
GAGAGTGTGTTTAGTTTTGTTT |
|
|
RASSF1A Un-R |
TAAACACTAACAAACACAAACCAAAC |
|
|
WIF1 NC_000012.12 |
WIF1 Me-F |
CGTTTTATTGGGCGTATCGT |
|
WIF1 Me-R |
ACTAACGCGAACGAAATACGA |
|
|
WIF1 Un-F |
GGGTGTTTTATTGGGTGTATTGT |
|
|
WIF1 Un-R |
AAAAAAACTAACACAAACAAAATACAAAC |
Me: Methylation; Un: Unmethylation; F: Forward; R: Reverse