Genes

Primer sequences (5′-3′)

β-Actin

F: TCCTTCCTGGGCATGGAG

R: AGGAGGAGCAATGATCTTGATCTT

RUNX2

F: AACTTCCTGTGCTCGGTGCTG

R: GGGGAGGATTTGTGAAGACGG

OPN

F: CTAGGCATCACCTGTGCCATACC

R: CTACTTAGACTACTTGACCAGTGAC

ON

F: GTGCAGAGGAAACCGAAGAG

R: AAGTGGCAGGAAGAGTCGAA

ALP

F: TAAGGACATCGCCTACCAGCTC

R: TCTTCCAGGTGTCAACGAGGT

LPL

F: AAAGCCCTGCTCGTGCTGAC

R: TAAACCGGGCCACATCCTGT

AP2

F: TGAGATTTCCTTCATACTGGG

R: TGGTTGATTTTCCATCCCATT

PPAR-γ

F: CGACCAGCTGAATCCAGAGT

R: GATGCGGATGGCCACCTCTT

ACAN

F: AGTTCTCAAATTGCATGGGGTGTC

R: AGTTCTCAAATTGCATGGGGTGTC

COL2-α1

F: CAGCCGCTTCACCTACAGC

R: TTTTGTATTCAATCACTGTCGCC

SOX9

F: CCCAACGCCATCTTCAAGG

R: CTGCTCAGCTCGCCGATGT

TGF-β3

F: GGTTTTCCGCTTCAATGTGT

R: TATAGCGCTGTTTGGCAATG

VIM

F: TCCGCACATTCGAGCAAAGA

R: TGATTCAAGTCTCAGCGGGC