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Table 1. Primer pairs used for DNA amplification and sequencing.

 

DNA barcode

Primer pairs

Reference

matK

matK-1RKim:

ACCCAGTCCATCTGGAAATCTTGGTTC

matK-3FKim:

CGTACAGTACTTTTGTGTTTACGAG

Costion et al. 2011

rbcL

rbcLa-F

ATGTCACCACAAACAGAGACTAAAGC

rbcLa-R

GTAAAATCAAGTCCACCRCG

Levin et al. 2003;

Kress and Erickson 2009

ITS (universal)

ITS92 AAGGTTTCCGTAGGTGAAC

ITS75 TATGCTTAAACTCAGCGGG

(ITS1, 5.8S-coding region dan ITS2)

Baldwin 1992

trnH–psbA

trnHf_05 CGCGCATGGTGGATTCACAATCC

psbA3_f. GTTATGCATGAACGTAATGCTC

Tate and Simpson 2003;

Sang et al. 1997