Table 1. Primer pairs used for DNA amplification and sequencing.
DNA barcode |
Primer pairs |
Reference |
matK |
matK-1RKim: ACCCAGTCCATCTGGAAATCTTGGTTC matK-3FKim: CGTACAGTACTTTTGTGTTTACGAG |
Costion et al. 2011 |
rbcL |
rbcLa-F ATGTCACCACAAACAGAGACTAAAGC rbcLa-R GTAAAATCAAGTCCACCRCG |
Levin et al. 2003; Kress and Erickson 2009 |
ITS (universal) |
ITS92 AAGGTTTCCGTAGGTGAAC ITS75 TATGCTTAAACTCAGCGGG (ITS1, 5.8S-coding region dan ITS2) |
Baldwin 1992 |
trnH–psbA |
trnHf_05 CGCGCATGGTGGATTCACAATCC psbA3_f. GTTATGCATGAACGTAATGCTC |
Tate and Simpson 2003; Sang et al. 1997 |