Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach

Table 1. Effect of test samples on the analgesic study of the METL on the acetic acid-induced writhing test in mice.

Treatments	Number of writhing
TWN 80 - 10 mg/mL	39.50 2.54^#
DFN – 10 mg/kg	13.20 1.98^***
METL - 200 mg/kg	36.25 2.38
METL - 400 mg/kg	27.13 1.32^*

The values were presented as mean ± SEM; One-way analysis of variance (ANOVA) was followed by Dunnett’s test. *P < 0.05, **P < 0.01 and ***P < 0.001, where # was designated as control. METL = methanol extract of Tetrastigma leucostaphylum leaves, TWN 80 = 1% Tween 80, and DFN = diclofenac sodium.