Culture medium with different combinations and concentrations of PGRs

Average initial length (cm) of individual shoot bud

Average length (cm) of

individual shoot bud after 30d of culture on elongation medium

Increase in length** (cm) of

shoot bud within 30d of culture on elongation medium

Average initial length (cm) of individual shoot bud

Average length (cm) of

individual shoot bud after 30d of culture on elongation medium

Increase in length** (cm) of

shoot bud within 30d of culture on elongation medium

Solid media

Liquid media

MS + 3% (w/v) sucrose + 1.0 mg/l IAA + 0.5 mg/l BAP

1.65±0.15

3.78±0.09

2.13±0.12

1.40±0.11

3.64±0.08

2.24±0.14

MS + 3% (w/v) sucrose + 0.5 mg/l IAA + 1.0 mg/l BAP

1.58±0.09

4.49±0.12

2.91±0.16

1.35±0.13

4.36±0.16

3.01±0.11

MS + 3% (w/v) sucrose + 1.0 mg/l IAA + 1.0 mg/l BAP

1.40±0.11

3.86±0.14

2.46±0.10

1.52±0.12

4.25±0.11

2.73±0.14

MS + 3% (w/v) sucrose + 1.0 mg/l  NAA + 0.5 mg/l BAP

1.45±0.19

3.50±0.19

2.05±0.13

1.45±0.18

4.07±0.14

2.62±0.16

MS+3% (w/v) sucrose + 0.5 mg/l  NAA+1.0 mg/l BAP

1.70±0.11

4.68±0.10

2.98±0.17

1.52±0.08

4.63±0.17

3.11±0.12

MS + 3% (w/v) sucrose + 1.0 mg/l  NAA + 1.0 mg/l BAP

1.55±0.14

4.17±0.18

2.62±0.08

1.70±0.14

4.75±0.19

3.05±0.17

MS + 3% (w/v) sucrose + 1.0 mg/l Pic + 0.5 mg/l BAP

1.65±0.17

4.02±0.09

2.37±0.11

1.65±0.12

4.27±0.16

2.62±0.19

MS+3% (w/v) sucrose + 0.5 mg/l  Pic  + 1.0 mg/l BAP

1.52±0.09

4.54±0.15

3.02±0.19

1.52±0.17

4.56±0.10

3.04±0.16

MS + 3% (w/v) sucrose + 1.0 mg/l Pic  + 1.0 mg/l BAP

1.48±0.09

5.24±0.11

3.76±0.14

1.45±0.15

4.36±0.09

2.91±0.10

PM + 2% (w/v) sucrose + 1.0 mg/l IAA + 0.5 mg/l BAP

1.45±0.15

3.47±0.07

2.02±0.12

1.50±0.10

3.95±0.06

2.45±0.07

PM + 2% (w/v) sucrose + 0.5 mg/l IAA + 1.0 mg/l BAP

1.40±0.10

4.25±0.19

2.85±0.16

1.65±0.07

4.61±0.12

2.96±0.08

PM + 2% (w/v) sucrose + 1.0 mg/l IAA + 1.0 mg/l BAP

1.55±0.17

4.04±0.14

2.49±0.11

1.70±0.09

4.27±0.15

2.57±0.11

PM + 2% (w/v) sucrose + 1.0 mg/l  NAA + 0.5 mg/l BAP

1.60±0.18

3.76±0.15

2.16±0.13

1.45±0.17

3.73±0.14

2.28±0.13

PM + 2% (w/v) sucrose + 0.5 mg/l  NAA + 1.0 mg/l BAP

1.65±0.13

4.56±0.16

2.91±0.18

1.70±0.10

4.71±0.17

3.01±0.8

PM + 2% (w/v) sucrose + 1.0 mg/l  NAA + 1.0 mg/l BAP

1.70±0.08

4.35±0.07

2.65±0.17

1.75±0.18

4.49±0.11

2.74±0.9

PM + 2% (w/v) sucrose + 1.0 mg/l Pic + 0.5 mg/l BAP

1.52±0.19

3.67±0.17

2.15±0.13

1.80±0.12

4.21±0.09

2.41±0.7

PM + 2% (w/v) sucrose + 0.5 mg/l  Pic  + 1.0 mg/l BAP

1.50±0.16

4.26±0.13

2.76±0.17

1.52±0.13

4.53±0.07

3.01±0.13

PM + 2% (w/v) sucrose + 1.0 mg/l  Pic  + 1.0 mg/l BAP

1.65±0.12

4.07±0.12

2.42±0.14

1.55±0.08

4.30±0.16

2.75±0.10