Combinations and

concentrations of PGRs

Explants

% of induced multiple shoot buds per segment

Time (d) required for sprouting of multiple shoot buds

Number of multiple shoot buds/ PLBs produced per segment

(Mean ± S.E.)

0.5 mg/l IAA + 1.0 mg/l BAP

PSU

40

35 - 38

3.81 ± 0.29

PSL

55

30 - 35

6.72 ± 0.48

0.5 mg/l IAA + 1.0 mg/l Kn

PSU

40

35 - 40

3.73 ± 0.24

PSL

45

35 - 38

5.36 ± 0.33

1.0 mg/l IAA + 2.0 mg/l BAP

PSU

45

35 - 40

5.07 ± 0.31

PSL

55

25 - 30

6.62 ± 0.44

1.0 mg/l IAA + 2.0 mg/l Kn

PSU

45

32 - 36

5.11 ± 0.30

PSL

50

30 - 35

6.17 ± 0.34

0.5 mg/l IBA + 1.0 mg/l BAP

PSU

35

35 - 40

3.68 ± 0.28

PSL

30

35 - 40

3.03 ± 0.21

0.5 mg/l IBA + 1.0 mg/l Kn

PSU

35

36 - 40

3.75 ± 0.25

PSL

40

32 - 36

4.11 ± 0.25

1.0 mg/l IBA + 2.0 mg/l BAP

PSU

45

32 - 35

5.03 ± 0.31

PSL

55

28 - 32

6.78 ± 0.39

1.0 mg/l IBA + 2.0 mg/l Kn

PSU

45

33 - 36

5.12 ± 0.28

PSL

50

32 - 35

6.08 ± 0.35

0.5 mg/l NAA + 1.0 mg/l BAP

PSU

40

30 - 35

3.69 ± 0.23

PSL

50

30 - 35

6.21 ± 0.41

0.5 mg/l NAA + 1.0 mg/l Kn

PSU

35

35 - 40

3.86 ± 0.25

PSL

40

35 - 38

4.23 ± 0.30

1.0 mg/l NAA + 2.0 mg/l BAP

PSU

55

25 - 30

6.43 ± 0.40

PSL

70

25 - 28

8.21 ± 0.44

1.0 mg/l NAA + 2.0 mg/l Kn

PSU

40

35 - 40

4.23 ± 0.32

PSL

50

32 - 35

5.97 ± 0.42

0.5 mg/l Pic + 1.0 mg/l BAP

PSU

35

35 - 40

3.78 ± 0.25

PSL

45

32 - 35

4.66 ± 0.29

0.5 mg/l Pic + 1.0 mg/l Kn

PSU

30

35 - 40

3.22 ± 0.19

PSL

45

33 - 36

4.56 ± 0.26

1.0 mg/l Pic + 2.0 mg/l BAP

PSU

50

25 - 30

5.96 ± 0.37

PSL

60

25 - 28

7.24 ± 0.41

1.0 mg/l Pic + 2.0 mg/l Kn

PSU

40

30 - 35

4.37 ± 0.25

PSL

50

28 - 33

6.02 ± 0.40