Developing Pseudomonas aeruginosa mutants with hyper-proteolytic activity through UV mutagenesis and characterization for optimized production

Table 3. UV-induced protease activity by mutant and parent strains of P. aeruginosa.

Irradiation period (min)	Strains	Protease activity (U/mL)^a
Irradiation period (min)	Strains	PA^b MZ2F	PA^b MZ4A
0	Wild	349.26±2.57	336.79±0.76
5	M05	342.27±3.17	334.27±0.78^c
10	M10	351.26±1.45	338.75±0.65
15	M15	343.26±0.62^d	337.09±2.17
20	M20	346.26±1.74	337.44±3.54
25	M25	612.84±2.50^e	339.13±1.16
30	M30	134.26±6.97^e	336.18±1.65

^aExperiments done in triplicate and data presented as mean±standard deviation; ^bPA stands for Pseudomonas aeruginosa; ^c,d,eProtease activity was considered significant at p≤0.05, p≤0.01, and p≤0.005 level, respectively.