Developing Pseudomonas aeruginosa mutants with hyper-proteolytic activity through UV mutagenesis and characterization for optimized production

Table 1. Relative proteolytic activity (RPA) of the wild-type bacterial strains.

Bacterial strains	Protease activity^a		RPA^bprofile
P. aeruginosa MZ2F		2.53±0.04^c	Good
P. aeruginosa MZ4A		2.12±0.12^d	Good
^aProtease activity by all bacterial strains was performed in triplicate and data expressed as mean±standard deviation;^bRPA value was used to classify strains based on enzyme activity as excellent (RPA > 5.0), good (2.0 ≤ RPA ≥ 5.0), or poor (RPA ≤ 2.0); ^c,dProtease production was significant at p≤0.05 and p≤0.01 level, respectively.