Table 1. Qualitative phytochemical screening of MeOH-CNE and HaE-CNE.
Phytochemical class |
Test performed |
Observations |
Results |
|
MeOH-CNE |
HaR-CNE |
|||
Alkaloids |
Dragendorff’s Test |
Turbidity/precipitation |
- |
+ |
Carbohydrates |
Molisch Test |
Formation of a purple product at the interface of the two layers |
+ |
++ |
Flavonoid |
Ferric chloride test |
Formation of yellow color which changed to colorless on acid addition |
+++ |
+++ |
Steroids |
Liebermann Burchard test |
Green to pink color was absent |
- |
- |
Terpenoids |
Liebermann Burchard test |
Appearance of reddish brown-deep red color |
- |
- |
Cardiac Glycosides |
Keller Killanic test |
Lower reddish-brown layer & upper acetic acid layer which turns bluish green |
+ |
+ |
Protein |
Burette’s Test |
Appearance of purple color |
++ |
++ |
(-) = not present; (+) = present in low concentration; (++) = present in moderately high concentration; (+++) = present in very high concentration. Where, MeOH-CNE: methanolic extract Cocos nucifera endocarp and HaE-CNE: hydro-alcoholic extract of Cocos nucifera endocarp.