Table 2. Frequency of shoot regeneration.
|
Media combinations |
Shoot regeneration frequency |
|
|
Cotyledon |
Hypocotyl |
|
|
MS + 0.5 mg/L 2,4-D |
0.00 ± 0.00 g |
0.00 ± 0.00 e |
|
MS + 1.0 mg/L 2,4-D |
0.00 ± 0.00 g |
0.00 ± 0.00 e |
|
MS + 0.5 mg/L 2,4-D + 1.0 mg/L BA |
26.66 ± 1.15 def |
13.33 ± 0.58 cde |
|
MS + 0.5 mg/L 2,4-D + 2.0 mg/L BA |
40.00 ± 1.00 cd |
20.00 ± 1.00 bcd |
|
MS + 0.5 mg/L 2,4-D + 3.0 mg/L BA |
46.66 ± 0.58 bc |
26.66 ± 1.15 abc |
|
MS + 1.0 mg/L 2,4-D + 1.0 mg/L BA |
13.33 ± 1.15 fg |
6.66 ± 0.58 de |
|
MS + 1.0 mg/L 2,4-D + 2.0 mg/L BA |
20.00 ± 1.00 ef |
13.33 ± 0.58 cde |
|
MS + 1.0 mg/L 2,4-D + 3.0 mg/L BA |
26.66 ± 0.58 def |
20.00 ± 1.00 bcd |
|
MS + 0.1 mg/L NAA + 1.0 mg/L BA |
40.00 1.00 cd |
13.33 ± 1.15 cde |
|
MS + 0.1 mg/L NAA + 2.0 mg/L BA |
46.66 ± 0.58 bc |
26.66 ± 0.58 abc |
|
MS + 0.1 mg/L NAA + 3.0 mg/L BA |
60.00 ± 1.00 ab |
33.33 ± 0.58 ab |
|
MS + 0.5 mg/L NAA + 1.0 mg/L BA |
40.00 ± 1.00 cd |
20.00 ± 1.00 bcd |
|
MS + 0.5 mg/L NAA + 2.0 mg/L BA |
66.66 ± 0.58 a |
26.66 ± 0.58 abc |
|
MS + 0.5 mg/L NAA + 3.0 mg/L BA |
73.33 ± 1.15 a |
40.00 ± 1.00 a |
|
MS + 1.0 mg/L NAA + 1.0 mg/L BA |
20.00 ± 1.00 ef |
13.33 ± 0.58 cde |
|
MS + 1.0 mg/L NAA + 2.0 mg/L BA |
26.66 ± 1.15 def |
20.00 ± 1.00 bcd |
|
MS + 1.0 mg/L NAA + 3.0 mg/L BA |
33.33 ± 0.58 cde |
26.66 ± 1.15 abc |
Frequency of shoot regeneration from 4 days old cotyledon and hypocotyl explants of B. napus cv. BARI sarisha-8 cultured in MS media complemented with various strengths of 2,4-D, NAA and BA.
Data consist of three replications and 10 explants were used for each replication. The mean values were compared by DMRT. Mean ± SD followed by similar letters aren't significantly diverse at P = 0.05.